FvH4_6g39331

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000161
Physical Location & Seq
Reverse (-)
31087261 .. 31091396
4136 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g39331.t1

Sequence Viewer

Length: 2598 bp
ATGCGCACCAACCTTCCAATTGCGTCATGGGGCTATGCAATATTGCATGCAGCCTTACTTATTCGTTTCAGACCCACTGCCAAACAACCATTCAGTGCGTACCAGTTGGTCACTGGATATGAGCCTGACATTTCGCATCTACGCATTTTCAGTTGTTCGGTTTATGTGCCAATTACGCCTCCACTGCGTTCTAAAATGGGTCCTCAGAGACGATTGGGTATCTACGTTGGTTATGAATCTCCAACGATTATCAGATATGTCGAGCCATTAACAGGTGATCTCTTTACCGCAAGGTTTGCGGATTGTCACTTTGATGAGACACTCTTCCCATCGTTAGGGGGAGATACGAACAAACGTGTTCATGTGGAACAACAGGAATTGTCGTGGTTTGTCCCCACTCTGTCTCATTATGATCCCCACACCTCACAATCTGAACGTGAAGTGAAACGTATTCTCGAACTTCAGAAAGTAGCAGATACGATGCCTGACTCTTTCTCCGAGATTGCGAAAGTGACAAGATCAGATATACCAGCTGCAAATGTTCCTGCTAGGCTCGAAGTCCCTAGAAAGGGCACGGCCATCCCCGGGCTCGGTACTGCCGCCGTCGACGGCGCCGCCCATGGTGGAGGGATGGAGGCCGTGGCTCCCCAAAGGAAAAGGGGGAGGCCACCTGGTTCGGTTGACATGCGTCCGAGAAAGAAACGGACCGACAAGGCACGAGTCAATCCTTTGATCATCGATGTTGAGAATCCTTCTCACGAGATCATCTCTGATTACAGTTATGTCCACGAGTTGATATTAGAATCCATGGGGACGCCTCAGGAATGCCAAAACAGAGCAGATTGGCCTAAATGGAAAGTTGCAATCCAGGCAGAATTAGACTCTCTAACAAAGAGACAGGTCTTCGGCCCAGTAGTGCTAACTCCGCCGAGTGTAAAGCCTGTTGGACATAAATGGGTCTTTGTTAGAAAGCGTAATGAGAAGAATGAGGTGCTTCGGTATAAGGCCCGCCTTGTGGCGCAAGGTTTCTCACAACGCCCTGGGATTGATTACGAGGAGACTTACTCTCCAGTCATGGACGTCATAACTTTCCGCTACTTAGTCAGTTTGGTAGTGTCCGAAAAACTTGACATGCAGCTCATGGATGTGGTTACGGCATATCTCTATGGGGATCTAGATTCAGAGATATATATGAAAGTGCCTGATGACATTACGTTACCTAAATCAAGTGACTCTAAACCACGGAGTGGCGTATGCTATAAGGTTGCAACGCTCACTTTACGGATTAAAGCAATCCGGGCGAATGTGAAGACAAGTTCCGGATTTGCAATAGTGGCCGTTTATGTCGATGACATGAACATTATCGGTACTCTTGATGAAATCAAAGAGACCGCGGCTTATCTGAAATCCAAGTTTGAGATTAAAGATCTTGGGAAGACTCGTTTTTGCCTAGGTCTTGAACTAGAGCACCGAGTCTGTGGGATCCTTATCCATCAGACTGCATATGTCCAAAAGATGCTCAGGCGATTTAATATGGACAAGATGCATCCTGTTAGCACTCCCATGATTGGTCGAAGTCTGGATATAACTAAAGATCCATTCCGTCCGAAAGAGGACGACGAAGAGGTCTTGAGCGCAGAGGTTCCATACCTTAGTGCAATAGGCGCGTTATTGTACTTAGCTCAATGCACTCGACCAGACATTGCATTCTCAGTCAACTTGTTAGCTAGATTTAGCTCAGCGCCAACCCAACGTCATTGGACCGGAGTTAAGAACATCTTTCGATATCTTAAAGGGACCATTGATTTGGGACTGTTCTTTCCCTACACTGATTCAAGAAGGACAGAAGATGGTCCTATGGCTCATGGAAGTCACGGCACCGATCCACCAAGGGACCGGACTGCCGCCATGGACGCCGCCGGTACGGCTGCTGGACACGGGTCCAATGCCGTGCACGCCACTGCCGCCTTGTCCTATGCCGAGACACCCAACAATACGTTGGTGGGTTTCGCCGACACCGGATATTTATCTGATCCTCATAAAGGACGTTCTCAAACTGGTTATGTTTTCACCATTAGGAATACGGCAATATCTTGGAGGTCTACAAAGCAGACTCTAGTGGCGACATCTTCAAATCATGCAGAGATTATAGCTTTGCATGAAGCTGTTCGGGAATGTGTTTGGTTGAGATCAATCATTAAGCACATTAGAGGAACTAGTGGATTAAGTTCTACAACTGATAGACCTACATGCATTTATGAGGACAATGCAGCTTGTATTGAGCAAATAAAGAGTACATATTCGTCAGCGAATTCTAGAGAAGCCAATGATCACGTGGGAATAGAACAAGAGTTCGCTGCAGATGAAGCCAATGATGACGGTGTTTTAGAGAAGAGCGGTTACGCAAGGCAAAGTGTCGAAAAGAGGCAGAATGTGCTTAGGCGCAAGCCAATTTGTGGCGGTCTCGATATTGTCACTAAGAGTTGGGTATTTAGGGTGCTTGCGGGTGGTTCAGTGGGTGTGGTGAGAGGTGGGATTAGAGCGAAGGAGGCCATGGTTTTGGGTTGGGTTAGGTTGGATGGTGGGTTTTCCCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

866

Amino Acids

96.13

Weight (kDa)

8.87

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 305 - 403 1.3e-26 Reverse transcriptase (RNA-dependent DNA polymerase)
RVT_2 PF07727 440 - 523 4.7e-14 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000161)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36192 FvH4_3g41702 FvH4_4g13933 FvH4_6g39331 FvH4_6g44542
malus_domestica MD17G1101700.v1.1
pyrus_communis pycom01g01810 pycom01g03340 pycom05g22610 pycom13g03220 pycom13g23770 pycom13g26870 pycom17g17400 pycom17g17410 pycom17g17890
rosa_chinensis RchiOBHm_Chr2g0129881 RchiOBHm_Chr2g0163801 RchiOBHm_Chr4g0392871 RchiOBHm_Chr7g0218471
rosa_multiflora Rmu_co8005914.1_g000001 Rmu_co8214666.1_g000002 Rmu_co8253889.1_g000001 Rmu_co8429419.1_g000001 Rmu_sc0000031.1_g000049 Rmu_sc0000091.1_g000004 Rmu_sc0000103.1_g000050 Rmu_sc0000147.1_g000047 Rmu_sc0000185.1_g000026 Rmu_sc0000235.1_g000056 Rmu_sc0000247.1_g000002 Rmu_sc0000310.1_g000010 Rmu_sc0000353.1_g000034 Rmu_sc0000353.1_g000035 Rmu_sc0000367.1_g000046 Rmu_sc0000420.1_g000034 Rmu_sc0000461.1_g000022 Rmu_sc0000544.1_g000007 Rmu_sc0000544.1_g000009 Rmu_sc0000607.1_g000032 Rmu_sc0000647.1_g000011 Rmu_sc0000675.1_g000049 Rmu_sc0000680.1_g000015 Rmu_sc0000795.1_g000112 Rmu_sc0000851.1_g000013 Rmu_sc0000868.1_g000019 Rmu_sc0000869.1_g000009 Rmu_sc0000898.1_g000061 Rmu_sc0000990.1_g000006 Rmu_sc0001009.1_g000006 Rmu_sc0001051.1_g000034 Rmu_sc0001067.1_g000005 Rmu_sc0001124.1_g000020 Rmu_sc0001142.1_g000016 Rmu_sc0001231.1_g000015 Rmu_sc0001235.1_g000013 Rmu_sc0001336.1_g000036 Rmu_sc0001552.1_g000033 Rmu_sc0001604.1_g000003 Rmu_sc0001605.1_g000009 Rmu_sc0001605.1_g000011 Rmu_sc0001668.1_g000025 Rmu_sc0001698.1_g000009 Rmu_sc0001721.1_g000010 Rmu_sc0001735.1_g000004 Rmu_sc0001832.1_g000017 Rmu_sc0001844.1_g000024 Rmu_sc0001880.1_g000026 Rmu_sc0001896.1_g000018 Rmu_sc0001918.1_g000011 Rmu_sc0002072.1_g000022 Rmu_sc0002109.1_g000003 Rmu_sc0002142.1_g000017 Rmu_sc0002166.1_g000001 Rmu_sc0002207.1_g000015 Rmu_sc0002346.1_g000001 Rmu_sc0002460.1_g000009 Rmu_sc0002460.1_g000043 Rmu_sc0002460.1_g000071 Rmu_sc0002672.1_g000001 Rmu_sc0002699.1_g000019 Rmu_sc0002736.1_g000007 Rmu_sc0002773.1_g000003 Rmu_sc0002893.1_g000006 Rmu_sc0002968.1_g000014 Rmu_sc0002968.1_g000015 Rmu_sc0003032.1_g000020 Rmu_sc0003044.1_g000024 Rmu_sc0003200.1_g000007 Rmu_sc0003261.1_g000031 Rmu_sc0003347.1_g000014 Rmu_sc0003385.1_g000019 Rmu_sc0003385.1_g000020 Rmu_sc0003419.1_g000017 Rmu_sc0003605.1_g000029 Rmu_sc0003679.1_g000010 Rmu_sc0003708.1_g000002 Rmu_sc0003737.1_g000033 Rmu_sc0003981.1_g000021 Rmu_sc0004101.1_g000009 Rmu_sc0004119.1_g000009 Rmu_sc0004248.1_g000021 Rmu_sc0004362.1_g000013 Rmu_sc0004520.1_g000020 Rmu_sc0004741.1_g000017 Rmu_sc0004771.1_g000007 Rmu_sc0004835.1_g000027 Rmu_sc0004969.1_g000010 Rmu_sc0005037.1_g000002 Rmu_sc0005272.1_g000006 Rmu_sc0005584.1_g000017 Rmu_sc0005652.1_g000005 Rmu_sc0005670.1_g000010 Rmu_sc0005781.1_g000035 Rmu_sc0005816.1_g000026 Rmu_sc0005852.1_g000003 Rmu_sc0006087.1_g000008 Rmu_sc0006152.1_g000005 Rmu_sc0006160.1_g000001 Rmu_sc0006313.1_g000014 Rmu_sc0006347.1_g000001 Rmu_sc0006583.1_g000024 Rmu_sc0006711.1_g000018 Rmu_sc0006952.1_g000003 Rmu_sc0006976.1_g000006 Rmu_sc0007115.1_g000006 Rmu_sc0007490.1_g000028 Rmu_sc0007740.1_g000003 Rmu_sc0007779.1_g000001 Rmu_sc0007907.1_g000003 Rmu_sc0007967.1_g000003 Rmu_sc0008456.1_g000006 Rmu_sc0008578.1_g000003 Rmu_sc0008642.1_g000001 Rmu_sc0008848.1_g000001 Rmu_sc0010065.1_g000009 Rmu_sc0010263.1_g000003 Rmu_sc0010632.1_g000004 Rmu_sc0011050.1_g000004 Rmu_sc0011563.1_g000005 Rmu_sc0011854.1_g000001 Rmu_sc0012216.1_g000003 Rmu_sc0012689.1_g000002 Rmu_sc0012945.1_g000001 Rmu_sc0013071.1_g000003 Rmu_sc0013305.1_g000008 Rmu_sc0013698.1_g000009 Rmu_sc0013772.1_g000007 Rmu_sc0013874.1_g000001 Rmu_sc0014217.1_g000001 Rmu_sc0014478.1_g000019 Rmu_sc0014724.1_g000002 Rmu_sc0016563.1_g000002 Rmu_sc0016563.1_g000003 Rmu_sc0017108.1_g000001 Rmu_sc0017408.1_g000002 Rmu_sc0020248.1_g000001 Rmu_sc0021416.1_g000005 Rmu_sc0022221.1_g000003 Rmu_sc0022402.1_g000002 Rmu_sc0023172.1_g000001 Rmu_sc0024783.1_g000001 Rmu_sc0028152.1_g000001 Rmu_sc0028541.1_g000001 Rmu_sc0029935.1_g000001 Rmu_sc0030041.1_g000001 Rmu_sc0035924.1_g000001 Rmu_sc0038113.1_g000002 Rmu_sc0040377.1_g000001 Rmu_ssc0000041.1_g000024 Rmu_ssc0000197.1_g000066 Rmu_ssc0000213.1_g000082 Rmu_ssc0000215.1_g000018
rosa_roxburghii Rroxscaffold_152G00434500 Rroxscaffold_3G00223490 Rroxscaffold_3G00226440 Rroxscaffold_3G00237960 Rroxscaffold_6G00394150
rosa_samantha Rh5CG379600 Rh5CG449200 Rh6DG448500
rosa_wichuraiana Rw0G004320 Rw1G010800 Rw2G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1625
AatII GACGTC 1 cut(s) 1083
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 2 cut(s) 611, 1877
AccB7I CCANNNNNTGG 2 cut(s) 1247, 2456
AccBSI CCGCTC 1 cut(s) 2397
AccI GTMKAC 2 cut(s) 606, 2102
AccII CGCG 2 cut(s) 1394, 1667
AccIII TCCGGA 1 cut(s) 1319
AclWI GGATC 7 cut(s) 407, 1179, 1477, 1490, 1589, 1877, 2027
AcoI YGGCCR 2 cut(s) 576, 1335
AcsI RAATTY 1 cut(s) 2311
AcuI CTGAAG 1 cut(s) 446
AcvI CACGTG 1 cut(s) 2335
AcyI GRCGYC 4 cut(s) 612, 815, 1080, 1914
AdeI CACNNNGTG 1 cut(s) 1247
AfaI GTAC 6 cut(s) 101, 595, 1369, 1676, 1924, 2296
AflIII ACRYGT 1 cut(s) 355
AgsI TTSAA 3 cut(s) 1460, 1836, 2133
AhlI ACTAGT 1 cut(s) 2216
AjnI CCWGG 3 cut(s) 670, 867, 1039
AjuI GAANNNNNNNTTGG 2 cut(s) 74, 106
AluBI AGCT 8 cut(s) 533, 1138, 1682, 1727, 1737, 2153, 2165, 2273
AluI AGCT 8 cut(s) 533, 1138, 1682, 1727, 1737, 2153, 2165, 2273
Alw21I GWGCWC 2 cut(s) 1470, 1956
Alw26I GTCTC 8 cut(s) 202, 311, 408, 889, 1052, 1382, 1976, 2468
Alw44I GTGCAC 1 cut(s) 1952
AlwI GGATC 7 cut(s) 407, 1179, 1477, 1490, 1589, 1877, 2027
Ama87I CYCGRG 1 cut(s) 584
Aor13HI TCCGGA 1 cut(s) 1319
AoxI GGCC 8 cut(s) 576, 636, 665, 845, 907, 1005, 1335, 2550
ApaLI GTGCAC 1 cut(s) 1952
ApeKI GCWGC 6 cut(s) 50, 533, 1135, 1928, 2270, 2357
ApoI RAATTY 1 cut(s) 2311
ArsI GACNNNNNNTTYG 2 cut(s) 1789, 1821
Asp700I GAANNNNTTC 1 cut(s) 2166
AspA2I CCTAGG 1 cut(s) 1450
AspLEI GCGC 7 cut(s) 6, 614, 1021, 1637, 1667, 1744, 2445
AspS9I GGNCC 9 cut(s) 200, 705, 908, 1006, 1761, 1797, 1853, 1894, 1941
AsuC2I CCSGG 3 cut(s) 585, 586, 1298
AsuHPI GGTGA 3 cut(s) 287, 2062, 2536
AvaI CYCGRG 1 cut(s) 584
AvaII GGWCC 7 cut(s) 200, 705, 1761, 1797, 1853, 1894, 1941
AvrII CCTAGG 1 cut(s) 1450
AxyI CCTNAGG 1 cut(s) 819
BaeGI GKGCMC 2 cut(s) 575, 1956
BamHI GGATCC 1 cut(s) 1482
BanI GGYRCC 2 cut(s) 611, 1877
BanII GRGCYC 1 cut(s) 591
BarI GAAGNNNNNNTAC 2 cut(s) 2384, 2416
BauI CACGAG 3 cut(s) 717, 758, 788
BbrPI CACGTG 1 cut(s) 2335
BbsI GAAGAC 3 cut(s) 895, 1316, 1442
Bbv12I GWGCWC 2 cut(s) 1470, 1956
BbvI GCAGC 6 cut(s) 62, 520, 1147, 1915, 2282, 2344
BccI CCATC 6 cut(s) 337, 587, 625, 1500, 1844, 2573
BciT130I CCWGG 3 cut(s) 672, 869, 1041
BclI TGATCA 2 cut(s) 732, 2329
BcnI CCSGG 3 cut(s) 585, 586, 1298
BcoDI GTCTC 8 cut(s) 202, 311, 408, 889, 1052, 1382, 1976, 2468
BcuI ACTAGT 1 cut(s) 2216
BfaI CTAG 9 cut(s) 549, 564, 1175, 1451, 1463, 1728, 2117, 2217, 2316
BfmI CTRYAG 1 cut(s) 2358
BfoI RGCGCY 2 cut(s) 615, 1745
BglI GCCNNNNNGGC 1 cut(s) 1925
BglII AGATCT 1 cut(s) 1426
BlnI CCTAGG 1 cut(s) 1450
BlpI GCTNAGC 1 cut(s) 1738
Bme1390I CCNGG 6 cut(s) 585, 586, 672, 869, 1041, 1298
Bme18I GGWCC 7 cut(s) 200, 705, 1761, 1797, 1853, 1894, 1941
BmeT110I CYCGRG 1 cut(s) 584
BmgT120I GGNCC 9 cut(s) 200, 705, 908, 1006, 1761, 1797, 1853, 1894, 1941
BmiI GGNNCC 9 cut(s) 201, 613, 645, 1484, 1644, 1798, 1879, 1895, 1942
BmrFI CCNGG 6 cut(s) 585, 586, 672, 869, 1041, 1298
BmrI ACTGGG 1 cut(s) 905
BmsI GCATC 5 cut(s) 145, 471, 1506, 1533, 1555
BmuI ACTGGG 1 cut(s) 905
BoxI GACNNNNGTC 2 cut(s) 687, 1939
BpiI GAAGAC 3 cut(s) 895, 1316, 1442
BplI GAGNNNNNCTC 4 cut(s) 752, 784, 1049, 1081
BpmI CTGGAG 1 cut(s) 1053
Bpu10I CCTNAGC 2 cut(s) 1520, 2438
Bpu1102I GCTNAGC 1 cut(s) 1738
BpuEI CTTGAG 1 cut(s) 1651
BpuMI CCSGG 3 cut(s) 585, 586, 1298
Bsa29I ATCGAT 1 cut(s) 738
BsaAI YACGTR 1 cut(s) 2335
BsaBI GATNNNNATC 2 cut(s) 1487, 2026
BsaHI GRCGYC 4 cut(s) 612, 815, 1080, 1914
BsaI GGTCTC 2 cut(s) 1382, 2468
BsaWI WCCGGW 4 cut(s) 1319, 1763, 1896, 2018
BsaXI ACNNNNNCTCC 4 cut(s) 479, 509, 1051, 1081
Bse118I RCCGGY 1 cut(s) 1919
Bse1I ACTGG 5 cut(s) 103, 118, 911, 1070, 2062
Bse21I CCTNAGG 1 cut(s) 819
Bse3DI GCAATG 1 cut(s) 1701
Bse8I GATNNNNATC 2 cut(s) 1487, 2026
BseAI TCCGGA 1 cut(s) 1319
BseBI CCWGG 3 cut(s) 672, 869, 1041
BseCI ATCGAT 1 cut(s) 738
BseGI GGATG 5 cut(s) 579, 636, 1150, 1546, 2584
BseJI GATNNNNATC 2 cut(s) 1487, 2026
BseMI GCAATG 1 cut(s) 1701
BseMII CTCAG 5 cut(s) 218, 833, 1534, 1725, 1752
BseNI ACTGG 5 cut(s) 103, 118, 911, 1070, 2062
BseRI GAGGAG 1 cut(s) 1070
BseSI GKGCMC 2 cut(s) 575, 1956
BseXI GCAGC 6 cut(s) 62, 520, 1147, 1915, 2282, 2344
Bsh1236I CGCG 2 cut(s) 1394, 1667
BshFI GGCC 8 cut(s) 578, 638, 667, 847, 909, 1007, 1337, 2552
BshNI GGYRCC 2 cut(s) 611, 1877
BshVI ATCGAT 1 cut(s) 738
BsiHKAI GWGCWC 2 cut(s) 1470, 1956
BsiHKCI CYCGRG 1 cut(s) 584
BsiSI CCGG 7 cut(s) 585, 1297, 1320, 1764, 1897, 1920, 2019
BslFI GGGAC 6 cut(s) 377, 545, 826, 1810, 1824, 1907
BsmAI GTCTC 8 cut(s) 202, 311, 408, 889, 1052, 1382, 1976, 2468
BsmBI CGTCTC 1 cut(s) 202
BsmFI GGGAC 6 cut(s) 377, 545, 826, 1810, 1824, 1907
BsmI GAATGC 2 cut(s) 830, 1706
BsnI GGCC 8 cut(s) 578, 638, 667, 847, 909, 1007, 1337, 2552
Bso31I GGTCTC 2 cut(s) 1382, 2468
BsoBI CYCGRG 1 cut(s) 584
Bsp1286I GDGCHC 4 cut(s) 575, 591, 1470, 1956
Bsp13I TCCGGA 1 cut(s) 1319
Bsp1720I GCTNAGC 1 cut(s) 1738
Bsp19I CCATGG 4 cut(s) 619, 807, 1908, 2553
BspANI GGCC 8 cut(s) 578, 638, 667, 847, 909, 1007, 1337, 2552
BspCNI CTCAG 5 cut(s) 217, 832, 1533, 1724, 1751
BspDI ATCGAT 1 cut(s) 738
BspEI TCCGGA 1 cut(s) 1319
BspFNI CGCG 2 cut(s) 1394, 1667
BspLI GGNNCC 9 cut(s) 201, 613, 645, 1484, 1644, 1798, 1879, 1895, 1942
BspMAI CTGCAG 1 cut(s) 2362
BspPI GGATC 7 cut(s) 407, 1179, 1477, 1490, 1589, 1877, 2027
BspQI GCTCTTC 1 cut(s) 2387
BspT107I GGYRCC 2 cut(s) 611, 1877
BspTNI GGTCTC 2 cut(s) 1382, 2468
BsrBI CCGCTC 1 cut(s) 2397
BsrDI GCAATG 1 cut(s) 1701
BsrFI RCCGGY 1 cut(s) 1919
BsrI ACTGG 5 cut(s) 103, 118, 911, 1070, 2062
BssAI RCCGGY 1 cut(s) 1919
BssNI GRCGYC 4 cut(s) 612, 815, 1080, 1914
BssSI CACGAG 3 cut(s) 717, 758, 788
BssT1I CCWWGG 6 cut(s) 619, 807, 1450, 1889, 1908, 2553
Bst2BI CACGAG 3 cut(s) 717, 758, 788
Bst2UI CCWGG 3 cut(s) 672, 869, 1041
Bst4CI ACNGT 3 cut(s) 779, 1815, 2381
Bst6I CTCTTC 3 cut(s) 329, 1617, 2387
BstACI GRCGYC 4 cut(s) 612, 815, 1080, 1914
BstAPI GCANNNNNTGC 2 cut(s) 296, 2434
BstBAI YACGTR 1 cut(s) 2335
BstC8I GCNNGC 5 cut(s) 48, 1009, 1956, 2447, 2502
BstDSI CCRYGG 7 cut(s) 619, 639, 807, 1241, 1392, 1908, 2553
BstENI CCTNNNNNAGG 1 cut(s) 2040
BstF5I GGATG 5 cut(s) 579, 636, 1150, 1546, 2584
BstFNI CGCG 2 cut(s) 1394, 1667
BstH2I RGCGCY 2 cut(s) 615, 1745
BstHHI GCGC 7 cut(s) 6, 614, 1021, 1637, 1667, 1744, 2445
BstMAI GTCTC 8 cut(s) 202, 311, 408, 889, 1052, 1382, 1976, 2468
BstNI CCWGG 3 cut(s) 672, 869, 1041
BstNSI RCATGY 4 cut(s) 50, 688, 1135, 2253
BstPAI GACNNNNGTC 2 cut(s) 687, 1939
BstSCI CCNGG 6 cut(s) 583, 584, 670, 867, 1039, 1296
BstSFI CTRYAG 1 cut(s) 2358
BstSLI GKGCMC 2 cut(s) 575, 1956
BstUI CGCG 2 cut(s) 1394, 1667
BstV1I GCAGC 6 cut(s) 62, 520, 1147, 1915, 2282, 2344
BstV2I GAAGAC 3 cut(s) 895, 1316, 1442
BstX2I RGATCY 4 cut(s) 1171, 1426, 1482, 1594
BstXI CCANNNNNNTGG 2 cut(s) 1807, 2560
BstYI RGATCY 4 cut(s) 1171, 1426, 1482, 1594
Bsu15I ATCGAT 1 cut(s) 738
Bsu36I CCTNAGG 1 cut(s) 819
BsuRI GGCC 8 cut(s) 578, 638, 667, 847, 909, 1007, 1337, 2552
BsuTUI ATCGAT 1 cut(s) 738
BtgI CCRYGG 7 cut(s) 619, 639, 807, 1241, 1392, 1908, 2553
BtsCI GGATG 5 cut(s) 579, 636, 1150, 1546, 2584
BtsI GCAGTG 3 cut(s) 75, 182, 1959
BtsIMutI CAGTG 7 cut(s) 75, 100, 111, 182, 1827, 1959, 2520
Cac8I GCNNGC 5 cut(s) 48, 1009, 1956, 2447, 2502
CfoI GCGC 7 cut(s) 6, 614, 1021, 1637, 1667, 1744, 2445
Cfr10I RCCGGY 1 cut(s) 1919
Cfr13I GGNCC 9 cut(s) 200, 705, 908, 1006, 1761, 1797, 1853, 1894, 1941
Cfr42I CCGCGG 1 cut(s) 1395
Cfr9I CCCGGG 1 cut(s) 584
ClaI ATCGAT 1 cut(s) 738
CpoI CGGWCCG 1 cut(s) 705
CseI GACGC 4 cut(s) 12, 677, 823, 1922
CsiI ACCWGGT 1 cut(s) 670
Csp6I GTAC 6 cut(s) 100, 594, 1368, 1675, 1923, 2295
CspI CGGWCCG 1 cut(s) 705
CviQI GTAC 6 cut(s) 100, 594, 1368, 1675, 1923, 2295
DinI GGCGCC 1 cut(s) 613
DraIII CACNNNGTG 1 cut(s) 1247
DrdI GACNNNNNNGTC 1 cut(s) 1625
DseDI GACNNNNNNGTC 1 cut(s) 1625
EaeI YGGCCR 2 cut(s) 576, 1335
Eam1104I CTCTTC 3 cut(s) 329, 1617, 2387
EarI CTCTTC 3 cut(s) 329, 1617, 2387
EciI GGCGGA 1 cut(s) 915
Eco130I CCWWGG 6 cut(s) 619, 807, 1450, 1889, 1908, 2553
Eco24I GRGCYC 1 cut(s) 591
Eco31I GGTCTC 2 cut(s) 1382, 2468
Eco32I GATATC 1 cut(s) 1787
Eco47I GGWCC 7 cut(s) 200, 705, 1761, 1797, 1853, 1894, 1941
Eco57I CTGAAG 1 cut(s) 446
Eco72I CACGTG 1 cut(s) 2335
Eco81I CCTNAGG 1 cut(s) 819
Eco88I CYCGRG 1 cut(s) 584
EcoNI CCTNNNNNAGG 1 cut(s) 2040
EcoO109I RGGNCCY 1 cut(s) 200
EcoRI GAATTC 1 cut(s) 2311
EcoRII CCWGG 3 cut(s) 670, 867, 1039
EcoRV GATATC 1 cut(s) 1787
EcoT14I CCWWGG 6 cut(s) 619, 807, 1450, 1889, 1908, 2553
EcoT22I ATGCAT 2 cut(s) 1548, 2255
EcoT38I GRGCYC 1 cut(s) 591
EgeI GGCGCC 1 cut(s) 613
EheI GGCGCC 1 cut(s) 613
ErhI CCWWGG 6 cut(s) 619, 807, 1450, 1889, 1908, 2553
Esp3I CGTCTC 1 cut(s) 202
FalI AAGNNNNNCTT 2 cut(s) 1763, 1795
FaqI GGGAC 6 cut(s) 377, 545, 826, 1810, 1824, 1907
FauI CCCGC 2 cut(s) 1016, 2497
FauNDI CATATG 1 cut(s) 1504
FbaI TGATCA 2 cut(s) 732, 2329
FblI GTMKAC 2 cut(s) 606, 2102
FokI GGATG 5 cut(s) 566, 643, 1157, 1533, 2591
FriOI GRGCYC 1 cut(s) 591
FspAI RTGCGCAY 1 cut(s) 5
FspBI CTAG 9 cut(s) 549, 564, 1175, 1451, 1463, 1728, 2117, 2217, 2316
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 7 cut(s) 5, 613, 1020, 1636, 1666, 1743, 2444
GsuI CTGGAG 1 cut(s) 1053
HaeII RGCGCY 2 cut(s) 615, 1745
HaeIII GGCC 8 cut(s) 578, 638, 667, 847, 909, 1007, 1337, 2552
HapII CCGG 7 cut(s) 585, 1297, 1320, 1764, 1897, 1920, 2019
HgaI GACGC 4 cut(s) 12, 677, 823, 1922
HhaI GCGC 7 cut(s) 6, 614, 1021, 1637, 1667, 1744, 2445
Hin1I GRCGYC 4 cut(s) 612, 815, 1080, 1914
Hin6I GCGC 7 cut(s) 4, 612, 1019, 1635, 1665, 1742, 2443
HinP1I GCGC 7 cut(s) 4, 612, 1019, 1635, 1665, 1742, 2443
HincII GTYRAC 3 cut(s) 607, 682, 1717
HindII GTYRAC 3 cut(s) 607, 682, 1717
HpaII CCGG 7 cut(s) 585, 1297, 1320, 1764, 1897, 1920, 2019
HphI GGTGA 3 cut(s) 287, 2062, 2536
Hpy166II GTNNAC 6 cut(s) 607, 682, 787, 1717, 1954, 2103
Hpy8I GTNNAC 6 cut(s) 607, 682, 787, 1717, 1954, 2103
Hpy99I CGWCG 3 cut(s) 608, 611, 1622
HpyAV CCTTC 4 cut(s) 23, 762, 1833, 2539
HpyCH4III ACNGT 3 cut(s) 779, 1815, 2381
Hsp92I GRCGYC 4 cut(s) 612, 815, 1080, 1914
HspAI GCGC 7 cut(s) 4, 612, 1019, 1635, 1665, 1742, 2443
KasI GGCGCC 1 cut(s) 611
Kpn2I TCCGGA 1 cut(s) 1319
Ksp22I TGATCA 2 cut(s) 732, 2329
KspI CCGCGG 1 cut(s) 1395
LguI GCTCTTC 1 cut(s) 2387
LmnI GCTCC 1 cut(s) 649
Lsp1109I GCAGC 6 cut(s) 62, 520, 1147, 1915, 2282, 2344
LweI GCATC 5 cut(s) 145, 471, 1506, 1533, 1555
MabI ACCWGGT 1 cut(s) 670
MaeI CTAG 9 cut(s) 549, 564, 1175, 1451, 1463, 1728, 2117, 2217, 2316
MaeIII GTNAC 9 cut(s) 109, 305, 511, 1150, 1215, 1229, 1871, 2399, 2473
MbiI CCGCTC 1 cut(s) 2397
MboII GAAGA 9 cut(s) 316, 895, 994, 1321, 1447, 1634, 1859, 2121, 2404
MfeI CAATTG 1 cut(s) 18
MflI RGATCY 4 cut(s) 1171, 1426, 1482, 1594
MhlI GDGCHC 4 cut(s) 575, 591, 1470, 1956
MluCI AATT 6 cut(s) 18, 171, 377, 875, 2311, 2451
Mly113I GGCGCC 1 cut(s) 612
MlyI GAGTC 7 cut(s) 482, 729, 875, 1226, 1432, 1482, 2107
MmeI TCCRAC 3 cut(s) 266, 925, 2556
Mph1103I ATGCAT 2 cut(s) 1548, 2255
MroI TCCGGA 1 cut(s) 1319
MroXI GAANNNNTTC 1 cut(s) 2166
MseI TTAA 8 cut(s) 269, 1287, 1422, 1530, 1770, 1791, 2199, 2225
MslI CAYNNNNRTG 3 cut(s) 312, 1145, 1562
MspA1I CMGCKG 2 cut(s) 533, 1394
MspI CCGG 7 cut(s) 585, 1297, 1320, 1764, 1897, 1920, 2019
MspR9I CCNGG 6 cut(s) 585, 586, 672, 869, 1041, 1298
MunI CAATTG 1 cut(s) 18
Mva1269I GAATGC 2 cut(s) 830, 1706
MvaI CCWGG 3 cut(s) 672, 869, 1041
MvnI CGCG 2 cut(s) 1394, 1667
NarI GGCGCC 1 cut(s) 612
NciI CCSGG 3 cut(s) 585, 586, 1298
NcoI CCATGG 4 cut(s) 619, 807, 1908, 2553
NdeI CATATG 1 cut(s) 1504
NlaIV GGNNCC 9 cut(s) 201, 613, 645, 1484, 1644, 1798, 1879, 1895, 1942
NmeAIII GCCGAG 2 cut(s) 954, 2005
NmuCI GTSAC 6 cut(s) 109, 305, 511, 1229, 1871, 2473
NsbI TGCGCA 1 cut(s) 5
NsiI ATGCAT 2 cut(s) 1548, 2255
NspI RCATGY 4 cut(s) 50, 688, 1135, 2253
PaeI GCATGC 1 cut(s) 50
PasI CCCWGGG 1 cut(s) 1040
PciSI GCTCTTC 1 cut(s) 2387
PctI GAATGC 2 cut(s) 830, 1706
PdmI GAANNNNTTC 1 cut(s) 2166
PfeI GAWTC 5 cut(s) 236, 748, 803, 1178, 1832
PflMI CCANNNNNTGG 2 cut(s) 1247, 2456
PleI GAGTC 7 cut(s) 482, 728, 875, 1226, 1432, 1481, 2107
PluTI GGCGCC 1 cut(s) 615
PmaCI CACGTG 1 cut(s) 2335
PmlI CACGTG 1 cut(s) 2335
PpsI GAGTC 7 cut(s) 482, 728, 875, 1226, 1432, 1481, 2107
Ppu21I YACGTR 1 cut(s) 2335
PpuMI RGGWCCY 1 cut(s) 200
PshAI GACNNNNGTC 2 cut(s) 687, 1939
Psp5II RGGWCCY 1 cut(s) 200
Psp6I CCWGG 3 cut(s) 670, 867, 1039
PspCI CACGTG 1 cut(s) 2335
PspGI CCWGG 3 cut(s) 670, 867, 1039
PspN4I GGNNCC 9 cut(s) 201, 613, 645, 1484, 1644, 1798, 1879, 1895, 1942
PspPI GGNCC 9 cut(s) 200, 705, 908, 1006, 1761, 1797, 1853, 1894, 1941
PspPPI RGGWCCY 1 cut(s) 200
PstI CTGCAG 1 cut(s) 2362
PsuI RGATCY 4 cut(s) 1171, 1426, 1482, 1594
PvuII CAGCTG 1 cut(s) 533
RsaI GTAC 6 cut(s) 101, 595, 1369, 1676, 1924, 2296
RsaNI GTAC 6 cut(s) 100, 594, 1368, 1675, 1923, 2295
RseI CAYNNNNRTG 3 cut(s) 312, 1145, 1562
Rsr2I CGGWCCG 1 cut(s) 705
RsrII CGGWCCG 1 cut(s) 705
SacII CCGCGG 1 cut(s) 1395
SalI GTCGAC 1 cut(s) 605
SapI GCTCTTC 1 cut(s) 2387
SaqAI TTAA 8 cut(s) 269, 1287, 1422, 1530, 1770, 1791, 2199, 2225
Sau96I GGNCC 9 cut(s) 200, 705, 908, 1006, 1761, 1797, 1853, 1894, 1941
SchI GAGTC 7 cut(s) 482, 729, 875, 1226, 1432, 1482, 2107
ScrFI CCNGG 6 cut(s) 585, 586, 672, 869, 1041, 1298
SduI GDGCHC 4 cut(s) 575, 591, 1470, 1956
SexAI ACCWGGT 1 cut(s) 670
SfaNI GCATC 5 cut(s) 145, 471, 1506, 1533, 1555
SfcI CTRYAG 1 cut(s) 2358
SfoI GGCGCC 1 cut(s) 613
Sfr303I CCGCGG 1 cut(s) 1395
SgrBI CCGCGG 1 cut(s) 1395
SgrDI CGTCGACG 1 cut(s) 605
SinI GGWCC 7 cut(s) 200, 705, 1761, 1797, 1853, 1894, 1941
SmaI CCCGGG 1 cut(s) 586
SmiMI CAYNNNNRTG 3 cut(s) 312, 1145, 1562
SmlI CTYRAG 1 cut(s) 1630
SmoI CTYRAG 1 cut(s) 1630
SpeI ACTAGT 1 cut(s) 2216
SphI GCATGC 1 cut(s) 50
Sse9I AATT 6 cut(s) 18, 171, 377, 875, 2311, 2451
SspDI GGCGCC 1 cut(s) 611
SspI AATATT 1 cut(s) 42
SspMI CTAG 9 cut(s) 549, 564, 1175, 1451, 1463, 1728, 2117, 2217, 2316
StyD4I CCNGG 6 cut(s) 583, 584, 670, 867, 1039, 1296
StyI CCWWGG 6 cut(s) 619, 807, 1450, 1889, 1908, 2553
TaaI ACNGT 3 cut(s) 779, 1815, 2381
TaqII GACCGA 1 cut(s) 722
TasI AATT 6 cut(s) 18, 171, 377, 875, 2311, 2451
TatI WGTACW 2 cut(s) 1674, 2294
TauI GCSGC 6 cut(s) 602, 617, 1397, 1907, 1919, 1967
TfiI GAWTC 5 cut(s) 236, 748, 803, 1178, 1832
Tru1I TTAA 8 cut(s) 269, 1287, 1422, 1530, 1770, 1791, 2199, 2225
Tru9I TTAA 8 cut(s) 269, 1287, 1422, 1530, 1770, 1791, 2199, 2225
TscAI CASTG 7 cut(s) 82, 100, 118, 189, 1834, 1966, 2520
TseFI GTSAC 6 cut(s) 109, 305, 511, 1229, 1871, 2473
TseI GCWGC 6 cut(s) 50, 533, 1135, 1928, 2270, 2357
Tsp45I GTSAC 6 cut(s) 109, 305, 511, 1229, 1871, 2473
TspDTI ATGAA 7 cut(s) 249, 350, 1208, 1370, 1392, 2175, 2379
TspGWI ACGGA 4 cut(s) 718, 1258, 1297, 1592
TspMI CCCGGG 1 cut(s) 584
TspRI CASTG 7 cut(s) 82, 100, 118, 189, 1834, 1966, 2520
Van91I CCANNNNNTGG 2 cut(s) 1247, 2456
VneI GTGCAC 1 cut(s) 1952
VpaK11BI GGWCC 7 cut(s) 200, 705, 1761, 1797, 1853, 1894, 1941
XagI CCTNNNNNAGG 1 cut(s) 2040
XapI RAATTY 1 cut(s) 2311
XbaI TCTAGA 2 cut(s) 1174, 2315
XceI RCATGY 4 cut(s) 50, 688, 1135, 2253
XcmI CCANNNNNNNNNTGG 4 cut(s) 24, 110, 1996, 2332
XmaI CCCGGG 1 cut(s) 584
XmaJI CCTAGG 1 cut(s) 1450
XmiI GTMKAC 2 cut(s) 606, 2102
XmnI GAANNNNTTC 1 cut(s) 2166
XspI CTAG 9 cut(s) 549, 564, 1175, 1451, 1463, 1728, 2117, 2217, 2316
ZraI GACGTC 1 cut(s) 1081
Zsp2I ATGCAT 2 cut(s) 1548, 2255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.