aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 16 |
| AciI | CCGC | 1 cut(s) | 56 |
| AclWI | GGATC | 1 cut(s) | 320 |
| AdeI | CACNNNGTG | 1 cut(s) | 221 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 16, 29, 178, 179, 180 |
| AgsI | TTSAA | 2 cut(s) | 279, 422 |
| AluBI | AGCT | 1 cut(s) | 144 |
| AluI | AGCT | 1 cut(s) | 144 |
| Alw26I | GTCTC | 1 cut(s) | 68 |
| AlwI | GGATC | 1 cut(s) | 320 |
| AoxI | GGCC | 1 cut(s) | 266 |
| ApeKI | GCWGC | 2 cut(s) | 144, 155 |
| AseI | ATTAAT | 1 cut(s) | 332 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 426 |
| AsuHPI | GGTGA | 1 cut(s) | 281 |
| BaeGI | GKGCMC | 1 cut(s) | 186 |
| BbvI | GCAGC | 2 cut(s) | 131, 142 |
| BcoDI | GTCTC | 1 cut(s) | 68 |
| BglII | AGATCT | 2 cut(s) | 34, 485 |
| BisI | GCNGC | 2 cut(s) | 145, 156 |
| BlsI | GCNGC | 2 cut(s) | 146, 157 |
| BmiI | GGNNCC | 2 cut(s) | 21, 246 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 338, 370 |
| BsaJI | CCNNGG | 1 cut(s) | 293 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 216, 246 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 16, 29, 178, 179, 180 |
| Bse1I | ACTGG | 1 cut(s) | 45 |
| BseDI | CCNNGG | 1 cut(s) | 293 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 16, 29, 178, 179, 180 |
| BseNI | ACTGG | 1 cut(s) | 45 |
| BseSI | GKGCMC | 1 cut(s) | 186 |
| BseXI | GCAGC | 2 cut(s) | 131, 142 |
| BshFI | GGCC | 1 cut(s) | 268 |
| BslFI | GGGAC | 1 cut(s) | 155 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 16, 29, 178, 179, 180 |
| BsmAI | GTCTC | 1 cut(s) | 68 |
| BsmFI | GGGAC | 1 cut(s) | 155 |
| BsnI | GGCC | 1 cut(s) | 268 |
| Bsp1286I | GDGCHC | 1 cut(s) | 186 |
| Bsp143I | GATC | 4 cut(s) | 34, 129, 325, 485 |
| BspACI | CCGC | 1 cut(s) | 56 |
| BspANI | GGCC | 1 cut(s) | 268 |
| BspHI | TCATGA | 1 cut(s) | 358 |
| BspLI | GGNNCC | 2 cut(s) | 21, 246 |
| BspPI | GGATC | 1 cut(s) | 320 |
| BsrI | ACTGG | 1 cut(s) | 45 |
| BssECI | CCNNGG | 1 cut(s) | 293 |
| BssMI | GATC | 4 cut(s) | 34, 129, 325, 485 |
| BssT1I | CCWWGG | 1 cut(s) | 293 |
| Bst6I | CTCTTC | 2 cut(s) | 251, 296 |
| BstDEI | CTNAG | 1 cut(s) | 218 |
| BstKTI | GATC | 4 cut(s) | 37, 132, 328, 488 |
| BstMAI | GTCTC | 1 cut(s) | 68 |
| BstMBI | GATC | 4 cut(s) | 34, 129, 325, 485 |
| BstSLI | GKGCMC | 1 cut(s) | 186 |
| BstV1I | GCAGC | 2 cut(s) | 131, 142 |
| BstX2I | RGATCY | 2 cut(s) | 34, 485 |
| BstYI | RGATCY | 2 cut(s) | 34, 485 |
| BsuRI | GGCC | 1 cut(s) | 268 |
| BtsI | GCAGTG | 2 cut(s) | 204, 246 |
| BtsIMutI | CAGTG | 2 cut(s) | 204, 246 |
| CciI | TCATGA | 1 cut(s) | 358 |
| CseI | GACGC | 1 cut(s) | 416 |
| CviAII | CATG | 2 cut(s) | 359, 389 |
| CviJI | RGCY | 3 cut(s) | 144, 245, 268 |
| CviKI_1 | RGCY | 3 cut(s) | 144, 245, 268 |
| DdeI | CTNAG | 1 cut(s) | 218 |
| DpnI | GATC | 4 cut(s) | 36, 131, 327, 487 |
| DpnII | GATC | 4 cut(s) | 34, 129, 325, 485 |
| DraIII | CACNNNGTG | 1 cut(s) | 221 |
| Eam1104I | CTCTTC | 2 cut(s) | 251, 296 |
| EarI | CTCTTC | 2 cut(s) | 251, 296 |
| Eco130I | CCWWGG | 1 cut(s) | 293 |
| EcoT14I | CCWWGG | 1 cut(s) | 293 |
| ErhI | CCWWGG | 1 cut(s) | 293 |
| FaeI | CATG | 2 cut(s) | 362, 392 |
| FaqI | GGGAC | 1 cut(s) | 155 |
| FatI | CATG | 2 cut(s) | 358, 388 |
| Fnu4HI | GCNGC | 2 cut(s) | 145, 156 |
| Fsp4HI | GCNGC | 2 cut(s) | 145, 156 |
| GluI | GCNGC | 2 cut(s) | 145, 156 |
| HaeIII | GGCC | 1 cut(s) | 268 |
| HgaI | GACGC | 1 cut(s) | 416 |
| Hin1II | CATG | 2 cut(s) | 362, 392 |
| HinfI | GANTC | 3 cut(s) | 349, 392, 427 |
| HphI | GGTGA | 1 cut(s) | 281 |
| Hpy188I | TCNGA | 1 cut(s) | 414 |
| Hpy188III | TCNNGA | 2 cut(s) | 359, 431 |
| Hpy99I | CGWCG | 1 cut(s) | 91 |
| HpyCH4V | TGCA | 3 cut(s) | 147, 158, 209 |
| HpyF3I | CTNAG | 1 cut(s) | 218 |
| Hsp92II | CATG | 2 cut(s) | 362, 392 |
| Kzo9I | GATC | 4 cut(s) | 34, 129, 325, 485 |
| LmnI | GCTCC | 2 cut(s) | 250, 415 |
| LpnPI | CCDG | 5 cut(s) | 15, 58, 154, 386, 444 |
| Lsp1109I | GCAGC | 2 cut(s) | 131, 142 |
| MaeIII | GTNAC | 2 cut(s) | 62, 122 |
| MalI | GATC | 4 cut(s) | 36, 131, 327, 487 |
| MboI | GATC | 4 cut(s) | 34, 129, 325, 485 |
| MboII | GAAGA | 5 cut(s) | 73, 268, 310, 313, 480 |
| MfeI | CAATTG | 1 cut(s) | 279 |
| MflI | RGATCY | 2 cut(s) | 34, 485 |
| MhlI | GDGCHC | 1 cut(s) | 186 |
| MluCI | AATT | 1 cut(s) | 279 |
| MnlI | CCTC | 8 cut(s) | 194, 218, 229, 252, 258, 297, 364, 397 |
| MroXI | GAANNNNTTC | 1 cut(s) | 426 |
| MseI | TTAA | 3 cut(s) | 92, 332, 476 |
| MslI | CAYNNNNRTG | 3 cut(s) | 69, 341, 501 |
| MspA1I | CMGCKG | 1 cut(s) | 144 |
| MunI | CAATTG | 1 cut(s) | 279 |
| NdeII | GATC | 4 cut(s) | 34, 129, 325, 485 |
| NlaIII | CATG | 2 cut(s) | 362, 392 |
| NlaIV | GGNNCC | 2 cut(s) | 21, 246 |
| NmuCI | GTSAC | 2 cut(s) | 62, 122 |
| PagI | TCATGA | 1 cut(s) | 358 |
| PdmI | GAANNNNTTC | 1 cut(s) | 426 |
| PfeI | GAWTC | 3 cut(s) | 349, 392, 427 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 16 |
| PkrI | GCNGC | 2 cut(s) | 146, 157 |
| PshBI | ATTAAT | 1 cut(s) | 332 |
| PspN4I | GGNNCC | 2 cut(s) | 21, 246 |
| PsuI | RGATCY | 2 cut(s) | 34, 485 |
| PvuII | CAGCTG | 1 cut(s) | 144 |
| RseI | CAYNNNNRTG | 3 cut(s) | 69, 341, 501 |
| SaqAI | TTAA | 3 cut(s) | 92, 332, 476 |
| SatI | GCNGC | 2 cut(s) | 145, 156 |
| Sau3AI | GATC | 4 cut(s) | 34, 129, 325, 485 |
| SduI | GDGCHC | 1 cut(s) | 186 |
| SetI | ASST | 3 cut(s) | 146, 164, 229 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 69, 341, 501 |
| Sse9I | AATT | 1 cut(s) | 279 |
| SsiI | CCGC | 1 cut(s) | 56 |
| StyI | CCWWGG | 1 cut(s) | 293 |
| TasI | AATT | 1 cut(s) | 279 |
| TfiI | GAWTC | 3 cut(s) | 349, 392, 427 |
| Tru1I | TTAA | 3 cut(s) | 92, 332, 476 |
| Tru9I | TTAA | 3 cut(s) | 92, 332, 476 |
| TscAI | CASTG | 2 cut(s) | 211, 246 |
| TseFI | GTSAC | 2 cut(s) | 62, 122 |
| TseI | GCWGC | 2 cut(s) | 144, 155 |
| Tsp45I | GTSAC | 2 cut(s) | 62, 122 |
| TspDTI | ATGAA | 1 cut(s) | 405 |
| TspRI | CASTG | 2 cut(s) | 211, 246 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 16 |
| VspI | ATTAAT | 1 cut(s) | 332 |
| XmnI | GAANNNNTTC | 1 cut(s) | 426 |