Rh5CG379600

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000161
Physical Location & Seq
Reverse (-)
49545781 .. 49552421
6641 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG379600.1

Sequence Viewer

Length: 3420 bp
ATGTTGTCCAATGGTACTGAATTTACCATTAATGAGGCCCTATACTCTCCACGTTCCAGAAGAACGTTATTGAGTTTTAAGGATATTCGAGCCAATGGTTATCACGTTGAAACCACTGAGGAAAAAGGAGTGGAATATCTTTGCATAACCTCCAATTTGTATGGCCGGAAGCGGACATTGGAGAAGCTAAAATGCCTCTCGAGTGGTCTCTACATGGCTACCATTAGATCAATTGAGGCGAATCACATAACCAGCCAGAAGCTAACCAATTCGAGCAACTACTTGCTTTGGCATGACCGTTTGGGACACCCAGGTCAAAGTATGATGCGCCGTATCCTAAATTCATCCCACGGGCATCCCCTTACCAATAAGGATCTTGTGTACAGTAACACATTATGCCAAGCTTGCTCATTGGGAAAATTAAATATAAGACCATCATATGCAAAGACTGAAAAAGACTCCAACCTTTTTCTACAAAGGATACAAGGGGATATATGTGGACCTATCCAACCACCATGCGGACCATTTAAATATTTCATGGTTTTGGTTGATGCATCGACAAGGTGGTCACATGTTACACTATTGTCCACAAGAAATGCTGCGTTTGCTAAACTCCTTGCCCAGATCATTAAATTAAGGGCTCACCACCCGGATTATCCAATCAAGTCCATAAGACTTGATAATGCCGGAGAGTTTACATCAAAATCTTTTGATGATTATTGCATGTCCCTTGGGATTAATGTTGAGCATCCAGTTCCCTATGTTCACACCCAGAATGGTCTCGCAGAAGCGTTCATTAAAAGATTACAAATGATCGCACGGACCTTGGTAATGCGCACCAAGCTTCCAGTATCTGCGTGGGGCTATGCAATTTTGCATGCAGCCATGTTGGTCCGACTAAGGCCCATTGCCACCCAACCTTATTCCGCGTTACAGTTGGTGACTGGGTACGAACCTGATGTTTCGCACTTACGTGTATTTGGGTGTGTAGTTTTTGTGCCAATTGCGCCGCCACAACGTAGAAAAATGGGTCCTCAACGAAGGATGGGCATATATGTCGGTTATGAATCCCCATCCATTATACGCTTTTTAGAGCCCTTGACAGGCGATCTCTTTACCGCTAGATTCGCGGATTGTCACTTTGATGAGACAGTCTTCCCGCCGTTAGGGGGAGATAAGAACGTTACCGTTCCTGATGAACGACGTGAATTGACGTGGAATGTCCCCACTATGTCTCATCTTGATCCCCGAACCGCACAATGCGATAATGAAGTGCGAAGAATTCTAGATCTACAGAGTGTAGCCCAAAATATGCCAGACGCTTTCTCTGATCTAGCTAAAGTGACGAGATCACATATACCTGCTGCAAATGTGCCTGCAAGAATAGATGTCCCTGTAGGACGCGTTGTCCCGGATGGACGAGGTACGACCATGGCGGCTAACCAGTCACATGTCCCTGCCCAGAAGCGAGGTAGACCACTAGGTTCGAAGGATTCCTATCCCCGGAAGAGGGTAAAACTAGCACAAACGAATCCACTAGACATCGCGATCTCAACTGATCCATCTCACGAGATAATTCCAGATTATGGGTCCGTCCTAGAAGAGACAACGTTGGGGGACGCTTCAACGTTTGAACCCACTCTCGAGAATAGAGAAATCTCGATAAATTATGCATGCTTAAGTGAGATTTGGAATCGAAGTGAGATTATCATCGATGATATATTTGTATTCGCAGTAGCTACTGAAATTATAACTAGCGATGATGTCGAACCTCGCTCTGTTGATGAATGTCAACGTAGAGACGACTGGCCAAAATGGAAAGAAGCAATCCAGGTCGAAATAGATTCCTTGACAAAGAGAAAAGTGTTCGGACCTGTCGTTCCCACACCTCACCATGTTAAACCTGTAGGATTTAAATGGGTATTTGTAAGGAAGCGTAATGAGGAAAACGAGATTGTAAGATACAAGGCTCGTCTAGTGGCGCAAGGATTCTCTCAACGCCCTGGGATTGATTACGATGAGACATATTCTCCTGTAATGGATGTTATAACGTTCCGCTACCTCATCAGTTTGGCAATTTCCGAAAAACTGAATATGCAGCTAATGGATGTGGTCACAGCTTATCTCTATGGGGATCTTGATACAGAGATATACATGAAAGTTCCTGAAGGACTTAAGATACCCAATACAAATAGTTCTAGACCACGGAACACCCTCTCCATTCGTTTGAGGCGTTCACTTTATGGATTGAAACAATCTGGACGGATGTGGTACAACCGTCTAAGTGAATATTTGATTGGGATGGGATATGCAAACAATGAACTATGCCCATGCGTGTTTATTAAGAAAACAAGTTCTGGATTTGTAATTGTGGCAGTTTATGTCGATGACATGAATCTGATTGGTACTCCTGAAGAGATCAAAGAAACCGCCAAGCACCTGAAGTCTGAATTCGAAATGAAAGATCTTGGGAGAACGAATTACTGCCTCGGCCTGGAGCTTGAGCACCGTGCTGATGGGATTCTGCTTCATCAATCAAACTACATCCAGAAGATGTTAAGACGCTTTAATGAGGATAAAGCGAAACCTTCAAGCACACCCATGGTCATCCGAAGTCTAGATCCTAAGAAAGATCCGTTTCGTCCCGCAGATAATAACGAAGAGATATTGGCACCAGAAGTCCCATATCTAAGTGCAATAGGCGCATTATTGTACTTGACTCAATGCACTAGACCAGACATTTCATTTTCTGTGAACTTGTTAGCTAGATATAGCTCTGCACCAACACGCCGCCACTGGAATGGTATAAAAGATATTTTTCGTTATCTTAGAGGTACGATTGATATGGGCTTATTTTATCCCTATGCATCAAGAAATGGATCAAACCCCCTTGATCCTCAGAATGATGCTCGCCTTGTTGGATATGCTGATGCAGGCTATCTATCAGACCCACACAAGGCACGTTCCCAAACTGGTTATGTCTTTACCATTGGGAATACGGCAATTTCTTGGAGGTCTACAAAACAGACCCTTGTTGCTACCTCTTCGAATCATGCTGAGATTCTAGCTCTTCACGAAGCAGTACGTGAATGTATATGGTTGAGAGCCATCACAAAGCATGTTCAAAGCACATGTGGACTGCATTCCACCACTGATGAACCAACCACTATCCATGAGGATAATGCTGCTTGCATCGAGCAAATGAAGACAGGTTTCATCAAAGGAGACAACACAAAACATATTGCACCAAAGTTTTTCTTCAATCAGCAACAACAGGAGCATCAGAAGATTGAGGTCAAGCAGATTCGATCTGAAGACAATCGTGCAGACCTCTTCACTAAGTCACTACCAAAATCTACATTCCAGAAGCATGTACAAGGTATTGGTCTACGTAAATTATCTGAATTACCTAACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

1139

Amino Acids

129.05

Weight (kDa)

8.93

Isoelectric Point (pI)

49.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
gag_pre-integrs PF13976 69 - 140 3.8e-12 GAG-pre-integrase domain
rve PF00665 159 - 253 1e-09 Integrase core domain
SH3_retrovirus PF25597 329 - 388 1.6e-06 Retroviral polymerase SH3-like domain
RVT_2 PF07727 626 - 869 4.2e-62 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000161)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36192 FvH4_3g41702 FvH4_4g13933 FvH4_6g39331 FvH4_6g44542
malus_domestica MD17G1101700.v1.1
pyrus_communis pycom01g01810 pycom01g03340 pycom05g22610 pycom13g03220 pycom13g23770 pycom13g26870 pycom17g17400 pycom17g17410 pycom17g17890
rosa_chinensis RchiOBHm_Chr2g0129881 RchiOBHm_Chr2g0163801 RchiOBHm_Chr4g0392871 RchiOBHm_Chr7g0218471
rosa_multiflora Rmu_co8005914.1_g000001 Rmu_co8214666.1_g000002 Rmu_co8253889.1_g000001 Rmu_co8429419.1_g000001 Rmu_sc0000031.1_g000049 Rmu_sc0000091.1_g000004 Rmu_sc0000103.1_g000050 Rmu_sc0000147.1_g000047 Rmu_sc0000185.1_g000026 Rmu_sc0000235.1_g000056 Rmu_sc0000247.1_g000002 Rmu_sc0000310.1_g000010 Rmu_sc0000353.1_g000034 Rmu_sc0000353.1_g000035 Rmu_sc0000367.1_g000046 Rmu_sc0000420.1_g000034 Rmu_sc0000461.1_g000022 Rmu_sc0000544.1_g000007 Rmu_sc0000544.1_g000009 Rmu_sc0000607.1_g000032 Rmu_sc0000647.1_g000011 Rmu_sc0000675.1_g000049 Rmu_sc0000680.1_g000015 Rmu_sc0000795.1_g000112 Rmu_sc0000851.1_g000013 Rmu_sc0000868.1_g000019 Rmu_sc0000869.1_g000009 Rmu_sc0000898.1_g000061 Rmu_sc0000990.1_g000006 Rmu_sc0001009.1_g000006 Rmu_sc0001051.1_g000034 Rmu_sc0001067.1_g000005 Rmu_sc0001124.1_g000020 Rmu_sc0001142.1_g000016 Rmu_sc0001231.1_g000015 Rmu_sc0001235.1_g000013 Rmu_sc0001336.1_g000036 Rmu_sc0001552.1_g000033 Rmu_sc0001604.1_g000003 Rmu_sc0001605.1_g000009 Rmu_sc0001605.1_g000011 Rmu_sc0001668.1_g000025 Rmu_sc0001698.1_g000009 Rmu_sc0001721.1_g000010 Rmu_sc0001735.1_g000004 Rmu_sc0001832.1_g000017 Rmu_sc0001844.1_g000024 Rmu_sc0001880.1_g000026 Rmu_sc0001896.1_g000018 Rmu_sc0001918.1_g000011 Rmu_sc0002072.1_g000022 Rmu_sc0002109.1_g000003 Rmu_sc0002142.1_g000017 Rmu_sc0002166.1_g000001 Rmu_sc0002207.1_g000015 Rmu_sc0002346.1_g000001 Rmu_sc0002460.1_g000009 Rmu_sc0002460.1_g000043 Rmu_sc0002460.1_g000071 Rmu_sc0002672.1_g000001 Rmu_sc0002699.1_g000019 Rmu_sc0002736.1_g000007 Rmu_sc0002773.1_g000003 Rmu_sc0002893.1_g000006 Rmu_sc0002968.1_g000014 Rmu_sc0002968.1_g000015 Rmu_sc0003032.1_g000020 Rmu_sc0003044.1_g000024 Rmu_sc0003200.1_g000007 Rmu_sc0003261.1_g000031 Rmu_sc0003347.1_g000014 Rmu_sc0003385.1_g000019 Rmu_sc0003385.1_g000020 Rmu_sc0003419.1_g000017 Rmu_sc0003605.1_g000029 Rmu_sc0003679.1_g000010 Rmu_sc0003708.1_g000002 Rmu_sc0003737.1_g000033 Rmu_sc0003981.1_g000021 Rmu_sc0004101.1_g000009 Rmu_sc0004119.1_g000009 Rmu_sc0004248.1_g000021 Rmu_sc0004362.1_g000013 Rmu_sc0004520.1_g000020 Rmu_sc0004741.1_g000017 Rmu_sc0004771.1_g000007 Rmu_sc0004835.1_g000027 Rmu_sc0004969.1_g000010 Rmu_sc0005037.1_g000002 Rmu_sc0005272.1_g000006 Rmu_sc0005584.1_g000017 Rmu_sc0005652.1_g000005 Rmu_sc0005670.1_g000010 Rmu_sc0005781.1_g000035 Rmu_sc0005816.1_g000026 Rmu_sc0005852.1_g000003 Rmu_sc0006087.1_g000008 Rmu_sc0006152.1_g000005 Rmu_sc0006160.1_g000001 Rmu_sc0006313.1_g000014 Rmu_sc0006347.1_g000001 Rmu_sc0006583.1_g000024 Rmu_sc0006711.1_g000018 Rmu_sc0006952.1_g000003 Rmu_sc0006976.1_g000006 Rmu_sc0007115.1_g000006 Rmu_sc0007490.1_g000028 Rmu_sc0007740.1_g000003 Rmu_sc0007779.1_g000001 Rmu_sc0007907.1_g000003 Rmu_sc0007967.1_g000003 Rmu_sc0008456.1_g000006 Rmu_sc0008578.1_g000003 Rmu_sc0008642.1_g000001 Rmu_sc0008848.1_g000001 Rmu_sc0010065.1_g000009 Rmu_sc0010263.1_g000003 Rmu_sc0010632.1_g000004 Rmu_sc0011050.1_g000004 Rmu_sc0011563.1_g000005 Rmu_sc0011854.1_g000001 Rmu_sc0012216.1_g000003 Rmu_sc0012689.1_g000002 Rmu_sc0012945.1_g000001 Rmu_sc0013071.1_g000003 Rmu_sc0013305.1_g000008 Rmu_sc0013698.1_g000009 Rmu_sc0013772.1_g000007 Rmu_sc0013874.1_g000001 Rmu_sc0014217.1_g000001 Rmu_sc0014478.1_g000019 Rmu_sc0014724.1_g000002 Rmu_sc0016563.1_g000002 Rmu_sc0016563.1_g000003 Rmu_sc0017108.1_g000001 Rmu_sc0017408.1_g000002 Rmu_sc0020248.1_g000001 Rmu_sc0021416.1_g000005 Rmu_sc0022221.1_g000003 Rmu_sc0022402.1_g000002 Rmu_sc0023172.1_g000001 Rmu_sc0024783.1_g000001 Rmu_sc0028152.1_g000001 Rmu_sc0028541.1_g000001 Rmu_sc0029935.1_g000001 Rmu_sc0030041.1_g000001 Rmu_sc0035924.1_g000001 Rmu_sc0038113.1_g000002 Rmu_sc0040377.1_g000001 Rmu_ssc0000041.1_g000024 Rmu_ssc0000197.1_g000066 Rmu_ssc0000213.1_g000082 Rmu_ssc0000215.1_g000018
rosa_roxburghii Rroxscaffold_152G00434500 Rroxscaffold_3G00223490 Rroxscaffold_3G00226440 Rroxscaffold_3G00237960 Rroxscaffold_6G00394150
rosa_samantha Rh5CG379600 Rh5CG449200 Rh6DG448500
rosa_wichuraiana Rw0G004320 Rw1G010800 Rw2G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1751, 2048
AasI GACNNNNNNGTC 2 cut(s) 312, 565
Acc16I TGCGCA 1 cut(s) 836
Acc36I ACCTGC 1 cut(s) 1369
AccB1I GGYRCC 1 cut(s) 2671
AccB7I CCANNNNNTGG 1 cut(s) 1586
AccI GTMKAC 3 cut(s) 1474, 3017, 3388
AccII CGCG 4 cut(s) 929, 1130, 1404, 1547
AclI AACGTT 5 cut(s) 65, 1182, 1610, 1628, 2051
AclWI GGATC 8 cut(s) 381, 1238, 1553, 2142, 2615, 2627, 2887, 2888
AcoI YGGCCR 2 cut(s) 163, 1808
AcsI RAATTY 4 cut(s) 20, 340, 1281, 2450
AcuI CTGAAG 4 cut(s) 2187, 2433, 2462, 3333
AflII CTTAAG 2 cut(s) 1678, 2174
AflIII ACRYGT 6 cut(s) 571, 973, 1402, 1450, 3131, 3414
AgsI TTSAA 7 cut(s) 110, 1626, 1634, 2251, 2592, 3125, 3262
AhdI GACNNNNNGTC 1 cut(s) 1406
AjiI CACGTC 2 cut(s) 1205, 1215
AjnI CCWGG 4 cut(s) 310, 1830, 2002, 2493
AjuI GAANNNNNNNTTGG 4 cut(s) 161, 193, 2280, 2312
AleI CACNNNNGTG 1 cut(s) 972
AloI GAACNNNNNNTCC 4 cut(s) 1863, 1895, 2201, 2233
Alw21I GWGCWC 1 cut(s) 2508
Alw26I GTCTC 8 cut(s) 212, 785, 1142, 1239, 1598, 1794, 2015, 3219
AlwI GGATC 8 cut(s) 381, 1238, 1553, 2142, 2615, 2627, 2887, 2888
AlwNI CAGNNNCTG 1 cut(s) 854
Ama87I CYCGRG 2 cut(s) 199, 1643
AoxI GGCC 5 cut(s) 36, 163, 902, 1808, 2491
ApeKI GCWGC 5 cut(s) 599, 881, 1364, 2098, 3185
ApoI RAATTY 4 cut(s) 20, 340, 1281, 2450
AseI ATTAAT 2 cut(s) 30, 738
AspLEI GCGC 5 cut(s) 330, 837, 1009, 1984, 2705
AspS9I GGNCC 9 cut(s) 37, 500, 521, 822, 892, 903, 1031, 1590, 1871
AsuC2I CCSGG 3 cut(s) 650, 1412, 1504
AsuHPI GGTGA 3 cut(s) 635, 952, 1883
AsuII TTCGAA 3 cut(s) 1487, 2454, 3047
AvaI CYCGRG 2 cut(s) 199, 1643
AvaII GGWCC 7 cut(s) 500, 521, 822, 892, 1031, 1590, 1871
BaeI ACNNNNGTAYC 2 cut(s) 2263, 2296
BalI TGGCCA 1 cut(s) 1810
BanI GGYRCC 1 cut(s) 2671
BanII GRGCYC 2 cut(s) 643, 1098
BauI CACGAG 1 cut(s) 1568
BbsI GAAGAC 3 cut(s) 1147, 3212, 3321
Bbv12I GWGCWC 1 cut(s) 2508
BbvI GCAGC 5 cut(s) 586, 893, 1351, 2110, 3172
BccI CCATC 8 cut(s) 442, 1039, 1081, 1409, 1570, 2296, 2510, 3116
BceAI ACGGC 3 cut(s) 315, 1147, 3015
BcgI CGANNNNNNTGC 2 cut(s) 1039, 1073
BciT130I CCWGG 4 cut(s) 312, 1832, 2004, 2495
BciVI GTATCC 2 cut(s) 344, 474
BcnI CCSGG 3 cut(s) 650, 1412, 1504
BcoDI GTCTC 8 cut(s) 212, 785, 1142, 1239, 1598, 1794, 2015, 3219
BfmI CTRYAG 3 cut(s) 1292, 1395, 1905
BfrI CTTAAG 2 cut(s) 1678, 2174
BfuAI ACCTGC 1 cut(s) 1369
BfuI GTATCC 2 cut(s) 344, 474
BglII AGATCT 2 cut(s) 1288, 2464
BisI GCNGC 8 cut(s) 600, 882, 1010, 1365, 1437, 2099, 2791, 3186
BlsI GCNGC 8 cut(s) 601, 883, 1011, 1366, 1438, 2100, 2792, 3187
Bme1390I CCNGG 7 cut(s) 312, 650, 1412, 1504, 1832, 2004, 2495
Bme18I GGWCC 7 cut(s) 500, 521, 822, 892, 1031, 1590, 1871
BmeRI GACNNNNNGTC 1 cut(s) 1406
BmeT110I CYCGRG 2 cut(s) 199, 1643
BmgBI CACGTC 2 cut(s) 1205, 1215
BmgT120I GGNCC 9 cut(s) 37, 500, 521, 822, 892, 903, 1031, 1590, 1871
BmiI GGNNCC 3 cut(s) 1032, 1591, 2673
BmrFI CCNGG 7 cut(s) 312, 650, 1412, 1504, 1832, 2004, 2495
BmrI ACTGGG 1 cut(s) 954
BmuI ACTGGG 1 cut(s) 954
BpiI GAAGAC 3 cut(s) 1147, 3212, 3321
BpmI CTGGAG 1 cut(s) 2516
Bpu14I TTCGAA 3 cut(s) 1487, 2454, 3047
BpuEI CTTGAG 1 cut(s) 2522
BpuMI CCSGG 3 cut(s) 650, 1412, 1504
Bsa29I ATCGAT 1 cut(s) 1713
BsaAI YACGTR 3 cut(s) 974, 3086, 3392
BsaBI GATNNNNATC 2 cut(s) 1497, 1709
BsaI GGTCTC 2 cut(s) 212, 785
BsaXI ACNNNNNCTCC 4 cut(s) 2014, 2044, 2201, 2231
Bse1I ACTGG 7 cut(s) 752, 848, 949, 1444, 1811, 2801, 2977
Bse3DI GCAATG 1 cut(s) 906
Bse8I GATNNNNATC 2 cut(s) 1497, 1709
BseBI CCWGG 4 cut(s) 312, 1832, 2004, 2495
BseCI ATCGAT 1 cut(s) 1713
BseJI GATNNNNATC 2 cut(s) 1497, 1709
BseMI GCAATG 1 cut(s) 906
BseMII CTCAG 3 cut(s) 108, 2912, 3048
BseNI ACTGG 7 cut(s) 752, 848, 949, 1444, 1811, 2801, 2977
BseXI GCAGC 5 cut(s) 586, 893, 1351, 2110, 3172
BsgI GTGCAG 2 cut(s) 2763, 3345
Bsh1236I CGCG 4 cut(s) 929, 1130, 1404, 1547
BshFI GGCC 5 cut(s) 38, 165, 904, 1810, 2493
BshNI GGYRCC 1 cut(s) 2671
BshVI ATCGAT 1 cut(s) 1713
BsiHKAI GWGCWC 1 cut(s) 2508
BsiHKCI CYCGRG 2 cut(s) 199, 1643
BsiSI CCGG 5 cut(s) 166, 650, 687, 1412, 1504
BslFI GGGAC 9 cut(s) 318, 712, 1208, 1376, 1394, 1439, 1631, 2628, 2666
BsmAI GTCTC 8 cut(s) 212, 785, 1142, 1239, 1598, 1794, 2015, 3219
BsmBI CGTCTC 1 cut(s) 1794
BsmFI GGGAC 9 cut(s) 318, 712, 1208, 1376, 1394, 1439, 1631, 2628, 2666
BsmI GAATGC 1 cut(s) 3142
BsnI GGCC 5 cut(s) 38, 165, 904, 1810, 2493
Bso31I GGTCTC 2 cut(s) 212, 785
BsoBI CYCGRG 2 cut(s) 199, 1643
Bsp119I TTCGAA 3 cut(s) 1487, 2454, 3047
Bsp1286I GDGCHC 3 cut(s) 643, 1098, 2508
Bsp1407I TGTACA 2 cut(s) 381, 3373
Bsp19I CCATGG 2 cut(s) 1431, 2601
Bsp68I TCGCGA 1 cut(s) 1547
BspANI GGCC 5 cut(s) 38, 165, 904, 1810, 2493
BspCNI CTCAG 3 cut(s) 109, 2911, 3049
BspDI ATCGAT 1 cut(s) 1713
BspFNI CGCG 4 cut(s) 929, 1130, 1404, 1547
BspLI GGNNCC 3 cut(s) 1032, 1591, 2673
BspMI ACCTGC 1 cut(s) 1369
BspPI GGATC 8 cut(s) 381, 1238, 1553, 2142, 2615, 2627, 2887, 2888
BspQI GCTCTTC 1 cut(s) 3075
BspT104I TTCGAA 3 cut(s) 1487, 2454, 3047
BspT107I GGYRCC 1 cut(s) 2671
BspTI CTTAAG 2 cut(s) 1678, 2174
BspTNI GGTCTC 2 cut(s) 212, 785
BsrDI GCAATG 1 cut(s) 906
BsrGI TGTACA 2 cut(s) 381, 3373
BsrI ACTGG 7 cut(s) 752, 848, 949, 1444, 1811, 2801, 2977
BssSI CACGAG 1 cut(s) 1568
BssT1I CCWWGG 4 cut(s) 730, 825, 1431, 2601
Bst2BI CACGAG 1 cut(s) 1568
Bst2UI CCWGG 4 cut(s) 312, 1832, 2004, 2495
Bst4CI ACNGT 7 cut(s) 299, 386, 936, 1153, 1189, 2279, 2510
Bst6I CTCTTC 7 cut(s) 1502, 1596, 2409, 2655, 3049, 3075, 3338
BstAFI CTTAAG 2 cut(s) 1678, 2174
BstAUI TGTACA 2 cut(s) 381, 3373
BstBAI YACGTR 3 cut(s) 974, 3086, 3392
BstBI TTCGAA 3 cut(s) 1487, 2454, 3047
BstC8I GCNNGC 7 cut(s) 406, 879, 1377, 1675, 2911, 2935, 3190
BstDEI CTNAG 9 cut(s) 117, 899, 2282, 2625, 2690, 2828, 2898, 3057, 3339
BstDSI CCRYGG 4 cut(s) 349, 1431, 2204, 2601
BstFNI CGCG 4 cut(s) 929, 1130, 1404, 1547
BstHHI GCGC 5 cut(s) 330, 837, 1009, 1984, 2705
BstMAI GTCTC 8 cut(s) 212, 785, 1142, 1239, 1598, 1794, 2015, 3219
BstMWI GCNNNNNNNGC 6 cut(s) 405, 605, 841, 1006, 1127, 2702
BstNI CCWGG 4 cut(s) 312, 1832, 2004, 2495
BstNSI RCATGY 9 cut(s) 575, 727, 881, 1454, 1677, 3122, 3135, 3374, 3418
BstSCI CCNGG 7 cut(s) 310, 648, 1410, 1502, 1830, 2002, 2493
BstSFI CTRYAG 3 cut(s) 1292, 1395, 1905
BstSNI TACGTA 1 cut(s) 3392
BstUI CGCG 4 cut(s) 929, 1130, 1404, 1547
BstV1I GCAGC 5 cut(s) 586, 893, 1351, 2110, 3172
BstV2I GAAGAC 3 cut(s) 1147, 3212, 3321
BstX2I RGATCY 6 cut(s) 373, 1288, 2134, 2464, 2620, 2632
BstXI CCANNNNNNTGG 1 cut(s) 2801
BstYI RGATCY 6 cut(s) 373, 1288, 2134, 2464, 2620, 2632
Bsu15I ATCGAT 1 cut(s) 1713
BsuI GTATCC 2 cut(s) 344, 474
BsuRI GGCC 5 cut(s) 38, 165, 904, 1810, 2493
BsuTUI ATCGAT 1 cut(s) 1713
BtgI CCRYGG 4 cut(s) 349, 1431, 2204, 2601
BtgZI GCGATG 2 cut(s) 1528, 1773
BtrI CACGTC 2 cut(s) 1205, 1215
BtsIMutI CAGTG 3 cut(s) 114, 2794, 3150
BtuMI TCGCGA 1 cut(s) 1547
BveI ACCTGC 1 cut(s) 1369
Cac8I GCNNGC 7 cut(s) 406, 879, 1377, 1675, 2911, 2935, 3190
CaiI CAGNNNCTG 1 cut(s) 854
CfoI GCGC 5 cut(s) 330, 837, 1009, 1984, 2705
Cfr13I GGNCC 9 cut(s) 37, 500, 521, 822, 892, 903, 1031, 1590, 1871
ClaI ATCGAT 1 cut(s) 1713
CseI GACGC 4 cut(s) 1328, 1410, 1628, 2571
DdeI CTNAG 9 cut(s) 117, 899, 2282, 2625, 2690, 2828, 2898, 3057, 3339
DraI TTTAAA 2 cut(s) 529, 1915
DrdI GACNNNNNNGTC 2 cut(s) 312, 565
DriI GACNNNNNGTC 1 cut(s) 1406
DseDI GACNNNNNNGTC 2 cut(s) 312, 565
EaeI YGGCCR 2 cut(s) 163, 1808
Eam1104I CTCTTC 7 cut(s) 1502, 1596, 2409, 2655, 3049, 3075, 3338
Eam1105I GACNNNNNGTC 1 cut(s) 1406
EarI CTCTTC 7 cut(s) 1502, 1596, 2409, 2655, 3049, 3075, 3338
Eco105I TACGTA 1 cut(s) 3392
Eco130I CCWWGG 4 cut(s) 730, 825, 1431, 2601
Eco24I GRGCYC 2 cut(s) 643, 1098
Eco31I GGTCTC 2 cut(s) 212, 785
Eco47I GGWCC 7 cut(s) 500, 521, 822, 892, 1031, 1590, 1871
Eco57I CTGAAG 4 cut(s) 2187, 2433, 2462, 3333
Eco88I CYCGRG 2 cut(s) 199, 1643
EcoO109I RGGNCCY 2 cut(s) 37, 1031
EcoRI GAATTC 2 cut(s) 1281, 2450
EcoRII CCWGG 4 cut(s) 310, 1830, 2002, 2493
EcoT14I CCWWGG 4 cut(s) 730, 825, 1431, 2601
EcoT22I ATGCAT 3 cut(s) 556, 1675, 2869
EcoT38I GRGCYC 2 cut(s) 643, 1098
ErhI CCWWGG 4 cut(s) 730, 825, 1431, 2601
Esp3I CGTCTC 1 cut(s) 1794
FalI AAGNNNNNCTT 2 cut(s) 3242, 3274
FaqI GGGAC 9 cut(s) 318, 712, 1208, 1376, 1394, 1439, 1631, 2628, 2666
FauI CCCGC 2 cut(s) 1167, 2653
FauNDI CATATG 1 cut(s) 439
FblI GTMKAC 3 cut(s) 1474, 3017, 3388
Fnu4HI GCNGC 8 cut(s) 600, 882, 1010, 1365, 1437, 2099, 2791, 3186
FriOI GRGCYC 2 cut(s) 643, 1098
Fsp4HI GCNGC 8 cut(s) 600, 882, 1010, 1365, 1437, 2099, 2791, 3186
FspAI RTGCGCAY 1 cut(s) 836
FspI TGCGCA 1 cut(s) 836
GlaI GCGC 5 cut(s) 329, 836, 1008, 1983, 2704
GluI GCNGC 8 cut(s) 600, 882, 1010, 1365, 1437, 2099, 2791, 3186
GsuI CTGGAG 1 cut(s) 2516
HaeIII GGCC 5 cut(s) 38, 165, 904, 1810, 2493
HapII CCGG 5 cut(s) 166, 650, 687, 1412, 1504
HgaI GACGC 4 cut(s) 1328, 1410, 1628, 2571
HhaI GCGC 5 cut(s) 330, 837, 1009, 1984, 2705
Hin6I GCGC 5 cut(s) 328, 835, 1007, 1982, 2703
HinP1I GCGC 5 cut(s) 328, 835, 1007, 1982, 2703
HincII GTYRAC 1 cut(s) 1793
HindII GTYRAC 1 cut(s) 1793
HindIII AAGCTT 2 cut(s) 402, 842
HpaII CCGG 5 cut(s) 166, 650, 687, 1412, 1504
HphI GGTGA 3 cut(s) 635, 952, 1883
Hpy99I CGWCG 1 cut(s) 1206
HpyAV CCTTC 4 cut(s) 1035, 1483, 2162, 2598
HpyCH4III ACNGT 7 cut(s) 299, 386, 936, 1153, 1189, 2279, 2510
HpyF10VI GCNNNNNNNGC 6 cut(s) 405, 605, 841, 1006, 1127, 2702
HpyF3I CTNAG 9 cut(s) 117, 899, 2282, 2625, 2690, 2828, 2898, 3057, 3339
HspAI GCGC 5 cut(s) 328, 835, 1007, 1982, 2703
LguI GCTCTTC 1 cut(s) 3075
LmnI GCTCC 2 cut(s) 2497, 3277
Lsp1109I GCAGC 5 cut(s) 586, 893, 1351, 2110, 3172
MfeI CAATTG 2 cut(s) 231, 1002
MflI RGATCY 6 cut(s) 373, 1288, 2134, 2464, 2620, 2632
MhlI GDGCHC 3 cut(s) 643, 1098, 2508
MlsI TGGCCA 1 cut(s) 1810
MluI ACGCGT 1 cut(s) 1402
MluNI TGGCCA 1 cut(s) 1810
MlyI GAGTC 2 cut(s) 452, 2713
MmeI TCCRAC 4 cut(s) 486, 532, 919, 2899
Mox20I TGGCCA 1 cut(s) 1810
Mph1103I ATGCAT 3 cut(s) 556, 1675, 2869
MscI TGGCCA 1 cut(s) 1810
MslI CAYNNNNRTG 4 cut(s) 972, 1143, 2600, 2799
Msp20I TGGCCA 1 cut(s) 1810
MspCI CTTAAG 2 cut(s) 1678, 2174
MspI CCGG 5 cut(s) 166, 650, 687, 1412, 1504
MspR9I CCNGG 7 cut(s) 312, 650, 1412, 1504, 1832, 2004, 2495
MunI CAATTG 2 cut(s) 231, 1002
Mva1269I GAATGC 1 cut(s) 3142
MvaI CCWGG 4 cut(s) 312, 1832, 2004, 2495
MvnI CGCG 4 cut(s) 929, 1130, 1404, 1547
MwoI GCNNNNNNNGC 6 cut(s) 405, 605, 841, 1006, 1127, 2702
NciI CCSGG 3 cut(s) 650, 1412, 1504
NcoI CCATGG 2 cut(s) 1431, 2601
NdeI CATATG 1 cut(s) 439
NlaIV GGNNCC 3 cut(s) 1032, 1591, 2673
NmeAIII GCCGAG 1 cut(s) 2469
NmuCI GTSAC 7 cut(s) 567, 940, 1136, 1342, 1446, 2113, 3342
NruI TCGCGA 1 cut(s) 1547
NsbI TGCGCA 1 cut(s) 836
NsiI ATGCAT 3 cut(s) 556, 1675, 2869
NspI RCATGY 9 cut(s) 575, 727, 881, 1454, 1677, 3122, 3135, 3374, 3418
NspV TTCGAA 3 cut(s) 1487, 2454, 3047
OliI CACNNNNGTG 1 cut(s) 972
PaeI GCATGC 2 cut(s) 881, 1677
PaeR7I CTCGAG 2 cut(s) 199, 1643
PasI CCCWGGG 1 cut(s) 2003
PciI ACATGT 4 cut(s) 571, 1450, 3131, 3414
PciSI GCTCTTC 1 cut(s) 3075
PcsI WCGNNNNNNNCGW 2 cut(s) 1875, 2230
PctI GAATGC 1 cut(s) 3142
PflMI CCANNNNNTGG 1 cut(s) 1586
PfoI TCCNGGA 1 cut(s) 1410
PkrI GCNGC 8 cut(s) 601, 883, 1011, 1366, 1438, 2100, 2792, 3187
PleI GAGTC 2 cut(s) 452, 2713
PpsI GAGTC 2 cut(s) 452, 2713
Ppu21I YACGTR 3 cut(s) 974, 3086, 3392
PpuMI RGGWCCY 1 cut(s) 1031
PscI ACATGT 4 cut(s) 571, 1450, 3131, 3414
PshBI ATTAAT 2 cut(s) 30, 738
PsiI TTATAA 2 cut(s) 1751, 2048
Psp1406I AACGTT 5 cut(s) 65, 1182, 1610, 1628, 2051
Psp5II RGGWCCY 1 cut(s) 1031
Psp6I CCWGG 4 cut(s) 310, 1830, 2002, 2493
PspGI CCWGG 4 cut(s) 310, 1830, 2002, 2493
PspN4I GGNNCC 3 cut(s) 1032, 1591, 2673
PspPI GGNCC 9 cut(s) 37, 500, 521, 822, 892, 903, 1031, 1590, 1871
PspPPI RGGWCCY 1 cut(s) 1031
PstNI CAGNNNCTG 1 cut(s) 854
PsuI RGATCY 6 cut(s) 373, 1288, 2134, 2464, 2620, 2632
RruI TCGCGA 1 cut(s) 1547
RseI CAYNNNNRTG 4 cut(s) 972, 1143, 2600, 2799
SapI GCTCTTC 1 cut(s) 3075
SatI GCNGC 8 cut(s) 600, 882, 1010, 1365, 1437, 2099, 2791, 3186
Sau96I GGNCC 9 cut(s) 37, 500, 521, 822, 892, 903, 1031, 1590, 1871
SchI GAGTC 2 cut(s) 452, 2713
ScrFI CCNGG 7 cut(s) 312, 650, 1412, 1504, 1832, 2004, 2495
SduI GDGCHC 3 cut(s) 643, 1098, 2508
SfcI CTRYAG 3 cut(s) 1292, 1395, 1905
Sfr274I CTCGAG 2 cut(s) 199, 1643
SfuI TTCGAA 3 cut(s) 1487, 2454, 3047
SinI GGWCC 7 cut(s) 500, 521, 822, 892, 1031, 1590, 1871
SlaI CTCGAG 2 cut(s) 199, 1643
SmiI ATTTAAAT 2 cut(s) 529, 1915
SmiMI CAYNNNNRTG 4 cut(s) 972, 1143, 2600, 2799
SmlI CTYRAG 5 cut(s) 199, 1643, 1678, 2174, 2501
SmoI CTYRAG 5 cut(s) 199, 1643, 1678, 2174, 2501
SnaBI TACGTA 1 cut(s) 3392
SphI GCATGC 2 cut(s) 881, 1677
SspI AATATT 2 cut(s) 533, 2291
StyD4I CCNGG 7 cut(s) 310, 648, 1410, 1502, 1830, 2002, 2493
StyI CCWWGG 4 cut(s) 730, 825, 1431, 2601
SwaI ATTTAAAT 2 cut(s) 529, 1915
TaaI ACNGT 7 cut(s) 299, 386, 936, 1153, 1189, 2279, 2510
TatI WGTACW 3 cut(s) 381, 2712, 3373
TauI GCSGC 3 cut(s) 1012, 1439, 2793
TscAI CASTG 3 cut(s) 121, 2801, 3157
TseFI GTSAC 7 cut(s) 567, 940, 1136, 1342, 1446, 2113, 3342
TseI GCWGC 5 cut(s) 599, 881, 1364, 2098, 3185
Tsp45I GTSAC 7 cut(s) 567, 940, 1136, 1342, 1446, 2113, 3342
TspGWI ACGGA 5 cut(s) 835, 1582, 2221, 2278, 2625
TspRI CASTG 3 cut(s) 121, 2801, 3157
Van91I CCANNNNNTGG 1 cut(s) 1586
Vha464I CTTAAG 2 cut(s) 1678, 2174
VpaK11BI GGWCC 7 cut(s) 500, 521, 822, 892, 1031, 1590, 1871
VspI ATTAAT 2 cut(s) 30, 738
XapI RAATTY 4 cut(s) 20, 340, 1281, 2450
XbaI TCTAGA 3 cut(s) 1285, 2198, 2617
XceI RCATGY 9 cut(s) 575, 727, 881, 1454, 1677, 3122, 3135, 3374, 3418
XcmI CCANNNNNNNNNTGG 1 cut(s) 855
XhoI CTCGAG 2 cut(s) 199, 1643
XmiI GTMKAC 3 cut(s) 1474, 3017, 3388
Zsp2I ATGCAT 3 cut(s) 556, 1675, 2869
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.