aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1012 |
| AccI | GTMKAC | 1 cut(s) | 50 |
| AciI | CCGC | 4 cut(s) | 473, 582, 623, 872 |
| AclI | AACGTT | 1 cut(s) | 170 |
| AclWI | GGATC | 1 cut(s) | 1013 |
| AcoI | YGGCCR | 1 cut(s) | 873 |
| AcsI | RAATTY | 1 cut(s) | 155 |
| AcuI | CTGAAG | 2 cut(s) | 920, 1007 |
| AfaI | GTAC | 3 cut(s) | 681, 709, 732 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 61, 546, 566, 629, 632 |
| AflIII | ACRYGT | 1 cut(s) | 34 |
| AgsI | TTSAA | 6 cut(s) | 203, 209, 272, 728, 752, 924 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 980, 1012 |
| AluBI | AGCT | 4 cut(s) | 104, 254, 281, 350 |
| AluI | AGCT | 4 cut(s) | 104, 254, 281, 350 |
| Alw21I | GWGCWC | 1 cut(s) | 106 |
| Alw26I | GTCTC | 2 cut(s) | 259, 605 |
| AlwI | GGATC | 1 cut(s) | 1013 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 317 |
| AoxI | GGCC | 3 cut(s) | 365, 493, 873 |
| ApaI | GGGCCC | 1 cut(s) | 497 |
| ApeKI | GCWGC | 2 cut(s) | 344, 347 |
| ApoI | RAATTY | 1 cut(s) | 155 |
| AseI | ATTAAT | 3 cut(s) | 668, 843, 1010 |
| AspS9I | GGNCC | 4 cut(s) | 4, 366, 493, 494 |
| AsuC2I | CCSGG | 1 cut(s) | 113 |
| AsuHPI | GGTGA | 4 cut(s) | 164, 525, 571, 581 |
| AsuII | TTCGAA | 1 cut(s) | 436 |
| AvaII | GGWCC | 1 cut(s) | 4 |
| BaeGI | GKGCMC | 1 cut(s) | 497 |
| BanII | GRGCYC | 2 cut(s) | 106, 497 |
| Bbv12I | GWGCWC | 1 cut(s) | 106 |
| BbvI | GCAGC | 2 cut(s) | 356, 359 |
| BccI | CCATC | 5 cut(s) | 21, 387, 438, 544, 892 |
| BceAI | ACGGC | 1 cut(s) | 610 |
| BcnI | CCSGG | 1 cut(s) | 113 |
| BcoDI | GTCTC | 2 cut(s) | 259, 605 |
| BfaI | CTAG | 2 cut(s) | 8, 939 |
| BfmI | CTRYAG | 1 cut(s) | 915 |
| BisI | GCNGC | 3 cut(s) | 345, 348, 873 |
| BlsI | GCNGC | 3 cut(s) | 346, 349, 874 |
| Bme1390I | CCNGG | 1 cut(s) | 113 |
| Bme18I | GGWCC | 1 cut(s) | 4 |
| BmgT120I | GGNCC | 4 cut(s) | 4, 366, 493, 494 |
| BmiI | GGNNCC | 2 cut(s) | 413, 495 |
| BmrFI | CCNGG | 1 cut(s) | 113 |
| BmsI | GCATC | 3 cut(s) | 4, 297, 766 |
| Bpu14I | TTCGAA | 1 cut(s) | 436 |
| BpuMI | CCSGG | 1 cut(s) | 113 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 61, 546, 566, 629, 632 |
| Bse118I | RCCGGY | 1 cut(s) | 832 |
| Bse1I | ACTGG | 1 cut(s) | 311 |
| BseGI | GGATG | 1 cut(s) | 536 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 61, 546, 566, 629, 632 |
| BseMII | CTCAG | 1 cut(s) | 98 |
| BseNI | ACTGG | 1 cut(s) | 311 |
| BseSI | GKGCMC | 1 cut(s) | 497 |
| BseXI | GCAGC | 2 cut(s) | 356, 359 |
| BseYI | CCCAGC | 1 cut(s) | 817 |
| BsgI | GTGCAG | 2 cut(s) | 728, 876 |
| BshFI | GGCC | 3 cut(s) | 367, 495, 875 |
| BsiHKAI | GWGCWC | 1 cut(s) | 106 |
| BsiSI | CCGG | 2 cut(s) | 113, 833 |
| BslFI | GGGAC | 1 cut(s) | 339 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 61, 546, 566, 629, 632 |
| BsmAI | GTCTC | 2 cut(s) | 259, 605 |
| BsmFI | GGGAC | 1 cut(s) | 339 |
| BsmI | GAATGC | 1 cut(s) | 779 |
| BsnI | GGCC | 3 cut(s) | 367, 495, 875 |
| Bsp119I | TTCGAA | 1 cut(s) | 436 |
| Bsp120I | GGGCCC | 1 cut(s) | 493 |
| Bsp1286I | GDGCHC | 2 cut(s) | 106, 497 |
| Bsp143I | GATC | 2 cut(s) | 806, 1018 |
| BspACI | CCGC | 4 cut(s) | 473, 582, 623, 872 |
| BspANI | GGCC | 3 cut(s) | 367, 495, 875 |
| BspCNI | CTCAG | 1 cut(s) | 97 |
| BspHI | TCATGA | 1 cut(s) | 1029 |
| BspLI | GGNNCC | 2 cut(s) | 413, 495 |
| BspPI | GGATC | 1 cut(s) | 1013 |
| BspT104I | TTCGAA | 1 cut(s) | 436 |
| BsrFI | RCCGGY | 1 cut(s) | 832 |
| BsrI | ACTGG | 1 cut(s) | 311 |
| BssAI | RCCGGY | 1 cut(s) | 832 |
| BssMI | GATC | 2 cut(s) | 806, 1018 |
| Bst4CI | ACNGT | 3 cut(s) | 588, 646, 679 |
| Bst6I | CTCTTC | 1 cut(s) | 983 |
| BstAPI | GCANNNNNTGC | 2 cut(s) | 590, 827 |
| BstBI | TTCGAA | 1 cut(s) | 436 |
| BstC8I | GCNNGC | 4 cut(s) | 283, 342, 416, 895 |
| BstDEI | CTNAG | 1 cut(s) | 84 |
| BstF5I | GGATG | 1 cut(s) | 536 |
| BstKTI | GATC | 2 cut(s) | 809, 1021 |
| BstMAI | GTCTC | 2 cut(s) | 259, 605 |
| BstMBI | GATC | 2 cut(s) | 806, 1018 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 590, 827 |
| BstNSI | RCATGY | 4 cut(s) | 38, 190, 332, 344 |
| BstSCI | CCNGG | 1 cut(s) | 111 |
| BstSFI | CTRYAG | 1 cut(s) | 915 |
| BstSLI | GKGCMC | 1 cut(s) | 497 |
| BstV1I | GCAGC | 2 cut(s) | 356, 359 |
| BsuRI | GGCC | 3 cut(s) | 367, 495, 875 |
| BtsCI | GGATG | 1 cut(s) | 536 |
| BtsIMutI | CAGTG | 1 cut(s) | 783 |
| Cac8I | GCNNGC | 4 cut(s) | 283, 342, 416, 895 |
| CaiI | CAGNNNCTG | 1 cut(s) | 317 |
| CciI | TCATGA | 1 cut(s) | 1029 |
| Cfr10I | RCCGGY | 1 cut(s) | 832 |
| Cfr13I | GGNCC | 4 cut(s) | 4, 366, 493, 494 |
| Csp6I | GTAC | 3 cut(s) | 680, 708, 731 |
| CviAII | CATG | 8 cut(s) | 35, 187, 224, 321, 329, 341, 562, 1030 |
| CviQI | GTAC | 3 cut(s) | 680, 708, 731 |
| DdeI | CTNAG | 1 cut(s) | 84 |
| DpnI | GATC | 2 cut(s) | 808, 1020 |
| DpnII | GATC | 2 cut(s) | 806, 1018 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1012 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1012 |
| EaeI | YGGCCR | 1 cut(s) | 873 |
| Eam1104I | CTCTTC | 1 cut(s) | 983 |
| EarI | CTCTTC | 1 cut(s) | 983 |
| Ecl136II | GAGCTC | 1 cut(s) | 104 |
| Eco24I | GRGCYC | 2 cut(s) | 106, 497 |
| Eco47I | GGWCC | 1 cut(s) | 4 |
| Eco53kI | GAGCTC | 1 cut(s) | 104 |
| Eco57I | CTGAAG | 2 cut(s) | 920, 1007 |
| EcoICRI | GAGCTC | 1 cut(s) | 104 |
| EcoO109I | RGGNCCY | 1 cut(s) | 494 |
| EcoT22I | ATGCAT | 1 cut(s) | 781 |
| EcoT38I | GRGCYC | 2 cut(s) | 106, 497 |
| FaeI | CATG | 8 cut(s) | 38, 190, 227, 324, 332, 344, 565, 1033 |
| FaqI | GGGAC | 1 cut(s) | 339 |
| FatI | CATG | 8 cut(s) | 34, 186, 223, 320, 328, 340, 561, 1029 |
| FauI | CCCGC | 1 cut(s) | 630 |
| FblI | GTMKAC | 1 cut(s) | 50 |
| Fnu4HI | GCNGC | 3 cut(s) | 345, 348, 873 |
| FokI | GGATG | 1 cut(s) | 523 |
| FriOI | GRGCYC | 2 cut(s) | 106, 497 |
| Fsp4HI | GCNGC | 3 cut(s) | 345, 348, 873 |
| FspBI | CTAG | 2 cut(s) | 8, 939 |
| GluI | GCNGC | 3 cut(s) | 345, 348, 873 |
| GsaI | CCCAGC | 1 cut(s) | 821 |
| HaeIII | GGCC | 3 cut(s) | 367, 495, 875 |
| HapII | CCGG | 2 cut(s) | 113, 833 |
| Hin1II | CATG | 8 cut(s) | 38, 190, 227, 324, 332, 344, 565, 1033 |
| HinfI | GANTC | 6 cut(s) | 513, 530, 739, 929, 967, 1033 |
| HpaII | CCGG | 2 cut(s) | 113, 833 |
| HphI | GGTGA | 4 cut(s) | 164, 525, 571, 581 |
| Hpy166II | GTNNAC | 2 cut(s) | 51, 675 |
| Hpy188I | TCNGA | 3 cut(s) | 87, 966, 1018 |
| Hpy188III | TCNNGA | 3 cut(s) | 662, 698, 1030 |
| Hpy8I | GTNNAC | 2 cut(s) | 51, 675 |
| Hpy99I | CGWCG | 2 cut(s) | 507, 663 |
| HpyAV | CCTTC | 3 cut(s) | 586, 729, 758 |
| HpyCH4III | ACNGT | 3 cut(s) | 588, 646, 679 |
| HpyCH4IV | ACGT | 3 cut(s) | 170, 502, 658 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 590, 827 |
| HpyF3I | CTNAG | 1 cut(s) | 84 |
| HpySE526I | ACGT | 3 cut(s) | 170, 502, 658 |
| Hsp92II | CATG | 8 cut(s) | 38, 190, 227, 324, 332, 344, 565, 1033 |
| Kzo9I | GATC | 2 cut(s) | 806, 1018 |
| LmnI | GCTCC | 1 cut(s) | 411 |
| Lsp1109I | GCAGC | 2 cut(s) | 356, 359 |
| LweI | GCATC | 3 cut(s) | 4, 297, 766 |
| MaeI | CTAG | 2 cut(s) | 8, 939 |
| MaeII | ACGT | 3 cut(s) | 170, 502, 658 |
| MaeIII | GTNAC | 2 cut(s) | 30, 799 |
| MalI | GATC | 2 cut(s) | 808, 1020 |
| MboI | GATC | 2 cut(s) | 806, 1018 |
| MboII | GAAGA | 2 cut(s) | 425, 1000 |
| MhlI | GDGCHC | 2 cut(s) | 106, 497 |
| MnlI | CCTC | 7 cut(s) | 28, 53, 231, 357, 507, 714, 939 |
| Mph1103I | ATGCAT | 1 cut(s) | 781 |
| MseI | TTAA | 6 cut(s) | 93, 261, 353, 668, 843, 1010 |
| MslI | CAYNNNNRTG | 2 cut(s) | 191, 606 |
| MspI | CCGG | 2 cut(s) | 113, 833 |
| MspR9I | CCNGG | 1 cut(s) | 113 |
| Mva1269I | GAATGC | 1 cut(s) | 779 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 590, 827 |
| NciI | CCSGG | 1 cut(s) | 113 |
| NdeII | GATC | 2 cut(s) | 806, 1018 |
| NlaIII | CATG | 8 cut(s) | 38, 190, 227, 324, 332, 344, 565, 1033 |
| NlaIV | GGNNCC | 2 cut(s) | 413, 495 |
| NmuCI | GTSAC | 2 cut(s) | 30, 799 |
| NsiI | ATGCAT | 1 cut(s) | 781 |
| NspI | RCATGY | 4 cut(s) | 38, 190, 332, 344 |
| NspV | TTCGAA | 1 cut(s) | 436 |
| PaeI | GCATGC | 1 cut(s) | 344 |
| PagI | TCATGA | 1 cut(s) | 1029 |
| PciI | ACATGT | 1 cut(s) | 34 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 901 |
| PctI | GAATGC | 1 cut(s) | 779 |
| PfeI | GAWTC | 6 cut(s) | 513, 530, 739, 929, 967, 1033 |
| PfoI | TCCNGGA | 1 cut(s) | 111 |
| PkrI | GCNGC | 3 cut(s) | 346, 349, 874 |
| PscI | ACATGT | 1 cut(s) | 34 |
| PshBI | ATTAAT | 3 cut(s) | 668, 843, 1010 |
| Psp124BI | GAGCTC | 1 cut(s) | 106 |
| Psp1406I | AACGTT | 1 cut(s) | 170 |
| PspFI | CCCAGC | 1 cut(s) | 817 |
| PspN4I | GGNNCC | 2 cut(s) | 413, 495 |
| PspOMI | GGGCCC | 1 cut(s) | 493 |
| PspPI | GGNCC | 4 cut(s) | 4, 366, 493, 494 |
| PstNI | CAGNNNCTG | 1 cut(s) | 317 |
| RsaI | GTAC | 3 cut(s) | 681, 709, 732 |
| RsaNI | GTAC | 3 cut(s) | 680, 708, 731 |
| RseI | CAYNNNNRTG | 2 cut(s) | 191, 606 |
| SacI | GAGCTC | 1 cut(s) | 106 |
| SaqAI | TTAA | 6 cut(s) | 93, 261, 353, 668, 843, 1010 |
| SatI | GCNGC | 3 cut(s) | 345, 348, 873 |
| Sau3AI | GATC | 2 cut(s) | 806, 1018 |
| Sau96I | GGNCC | 4 cut(s) | 4, 366, 493, 494 |
| ScrFI | CCNGG | 1 cut(s) | 113 |
| SduI | GDGCHC | 2 cut(s) | 106, 497 |
| SfaNI | GCATC | 3 cut(s) | 4, 297, 766 |
| SfcI | CTRYAG | 1 cut(s) | 915 |
| SfuI | TTCGAA | 1 cut(s) | 436 |
| SinI | GGWCC | 1 cut(s) | 4 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 191, 606 |
| SphI | GCATGC | 1 cut(s) | 344 |
| SsiI | CCGC | 4 cut(s) | 473, 582, 623, 872 |
| SspI | AATATT | 4 cut(s) | 386, 618, 757, 996 |
| SspMI | CTAG | 2 cut(s) | 8, 939 |
| SstI | GAGCTC | 1 cut(s) | 106 |
| StyD4I | CCNGG | 1 cut(s) | 111 |
| TaaI | ACNGT | 3 cut(s) | 588, 646, 679 |
| TaiI | ACGT | 3 cut(s) | 173, 505, 661 |
| TaqI | TCGA | 2 cut(s) | 436, 505 |
| TauI | GCSGC | 1 cut(s) | 875 |
| TfiI | GAWTC | 6 cut(s) | 513, 530, 739, 929, 967, 1033 |
| Tru1I | TTAA | 6 cut(s) | 93, 261, 353, 668, 843, 1010 |
| Tru9I | TTAA | 6 cut(s) | 93, 261, 353, 668, 843, 1010 |
| TscAI | CASTG | 1 cut(s) | 790 |
| TseFI | GTSAC | 2 cut(s) | 30, 799 |
| TseI | GCWGC | 2 cut(s) | 344, 347 |
| Tsp45I | GTSAC | 2 cut(s) | 30, 799 |
| TspDTI | ATGAA | 5 cut(s) | 218, 311, 669, 990, 1018 |
| TspRI | CASTG | 1 cut(s) | 790 |
| VpaK11BI | GGWCC | 1 cut(s) | 4 |
| VspI | ATTAAT | 3 cut(s) | 668, 843, 1010 |
| XapI | RAATTY | 1 cut(s) | 155 |
| XceI | RCATGY | 4 cut(s) | 38, 190, 332, 344 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 318 |
| XmiI | GTMKAC | 1 cut(s) | 50 |
| XspI | CTAG | 2 cut(s) | 8, 939 |
| Zsp2I | ATGCAT | 1 cut(s) | 781 |