Rmu_sc0022221.1_g000003

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0022221.1
OG0000161
Physical Location & Seq
Reverse (-)
4510 .. 5229
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0022221.1_g000003.1.cds

Sequence Viewer

Length: 597 bp
atgacaagtgatctctttactgctagatttgcggattgttactttgatgagacagtcttccagtcgttagggggagataggaaaaatgattttccaagggaacgacatgaattgtcgtggtttggcctcaatctgtctcattttgatccccgcacttcacaatgtgaaagtgaagtgaaaagaatagtcgatcttcaaaaagtagcagattcgatgcctgatgcgtttactgatatcgcaaaagtgactagagcacatatatcagctgcaaatgtgcctgcaaggttagtagtccccaacaaggggcatggtgccgcagatagaggcactgcaactacacttagtggaggtgtggttgaggtcgtggctccccaatggaagaggcggaggccacttggttcgattgacactcacccaaggaagaagagggcgaagaacaaagaaatctcaatggattatgagagtgcgtatgagttgatggaaagatcttccatacacattgatgatgtatttgcatatattgttgctcaagaaatcatagagcacgatgatatcgaactacgctatattgcagaatgtcaacaaagagcagattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

198

Amino Acids

22.47

Weight (kDa)

5.82

Isoelectric Point (pI)

50.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000161)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36192 FvH4_3g41702 FvH4_4g13933 FvH4_6g39331 FvH4_6g44542
malus_domestica MD17G1101700.v1.1
pyrus_communis pycom01g01810 pycom01g03340 pycom05g22610 pycom13g03220 pycom13g23770 pycom13g26870 pycom17g17400 pycom17g17410 pycom17g17890
rosa_chinensis RchiOBHm_Chr2g0129881 RchiOBHm_Chr2g0163801 RchiOBHm_Chr4g0392871 RchiOBHm_Chr7g0218471
rosa_multiflora Rmu_co8005914.1_g000001 Rmu_co8214666.1_g000002 Rmu_co8253889.1_g000001 Rmu_co8429419.1_g000001 Rmu_sc0000031.1_g000049 Rmu_sc0000091.1_g000004 Rmu_sc0000103.1_g000050 Rmu_sc0000147.1_g000047 Rmu_sc0000185.1_g000026 Rmu_sc0000235.1_g000056 Rmu_sc0000247.1_g000002 Rmu_sc0000310.1_g000010 Rmu_sc0000353.1_g000034 Rmu_sc0000353.1_g000035 Rmu_sc0000367.1_g000046 Rmu_sc0000420.1_g000034 Rmu_sc0000461.1_g000022 Rmu_sc0000544.1_g000007 Rmu_sc0000544.1_g000009 Rmu_sc0000607.1_g000032 Rmu_sc0000647.1_g000011 Rmu_sc0000675.1_g000049 Rmu_sc0000680.1_g000015 Rmu_sc0000795.1_g000112 Rmu_sc0000851.1_g000013 Rmu_sc0000868.1_g000019 Rmu_sc0000869.1_g000009 Rmu_sc0000898.1_g000061 Rmu_sc0000990.1_g000006 Rmu_sc0001009.1_g000006 Rmu_sc0001051.1_g000034 Rmu_sc0001067.1_g000005 Rmu_sc0001124.1_g000020 Rmu_sc0001142.1_g000016 Rmu_sc0001231.1_g000015 Rmu_sc0001235.1_g000013 Rmu_sc0001336.1_g000036 Rmu_sc0001552.1_g000033 Rmu_sc0001604.1_g000003 Rmu_sc0001605.1_g000009 Rmu_sc0001605.1_g000011 Rmu_sc0001668.1_g000025 Rmu_sc0001698.1_g000009 Rmu_sc0001721.1_g000010 Rmu_sc0001735.1_g000004 Rmu_sc0001832.1_g000017 Rmu_sc0001844.1_g000024 Rmu_sc0001880.1_g000026 Rmu_sc0001896.1_g000018 Rmu_sc0001918.1_g000011 Rmu_sc0002072.1_g000022 Rmu_sc0002109.1_g000003 Rmu_sc0002142.1_g000017 Rmu_sc0002166.1_g000001 Rmu_sc0002207.1_g000015 Rmu_sc0002346.1_g000001 Rmu_sc0002460.1_g000009 Rmu_sc0002460.1_g000043 Rmu_sc0002460.1_g000071 Rmu_sc0002672.1_g000001 Rmu_sc0002699.1_g000019 Rmu_sc0002736.1_g000007 Rmu_sc0002773.1_g000003 Rmu_sc0002893.1_g000006 Rmu_sc0002968.1_g000014 Rmu_sc0002968.1_g000015 Rmu_sc0003032.1_g000020 Rmu_sc0003044.1_g000024 Rmu_sc0003200.1_g000007 Rmu_sc0003261.1_g000031 Rmu_sc0003347.1_g000014 Rmu_sc0003385.1_g000019 Rmu_sc0003385.1_g000020 Rmu_sc0003419.1_g000017 Rmu_sc0003605.1_g000029 Rmu_sc0003679.1_g000010 Rmu_sc0003708.1_g000002 Rmu_sc0003737.1_g000033 Rmu_sc0003981.1_g000021 Rmu_sc0004101.1_g000009 Rmu_sc0004119.1_g000009 Rmu_sc0004248.1_g000021 Rmu_sc0004362.1_g000013 Rmu_sc0004520.1_g000020 Rmu_sc0004741.1_g000017 Rmu_sc0004771.1_g000007 Rmu_sc0004835.1_g000027 Rmu_sc0004969.1_g000010 Rmu_sc0005037.1_g000002 Rmu_sc0005272.1_g000006 Rmu_sc0005584.1_g000017 Rmu_sc0005652.1_g000005 Rmu_sc0005670.1_g000010 Rmu_sc0005781.1_g000035 Rmu_sc0005816.1_g000026 Rmu_sc0005852.1_g000003 Rmu_sc0006087.1_g000008 Rmu_sc0006152.1_g000005 Rmu_sc0006160.1_g000001 Rmu_sc0006313.1_g000014 Rmu_sc0006347.1_g000001 Rmu_sc0006583.1_g000024 Rmu_sc0006711.1_g000018 Rmu_sc0006952.1_g000003 Rmu_sc0006976.1_g000006 Rmu_sc0007115.1_g000006 Rmu_sc0007490.1_g000028 Rmu_sc0007740.1_g000003 Rmu_sc0007779.1_g000001 Rmu_sc0007907.1_g000003 Rmu_sc0007967.1_g000003 Rmu_sc0008456.1_g000006 Rmu_sc0008578.1_g000003 Rmu_sc0008642.1_g000001 Rmu_sc0008848.1_g000001 Rmu_sc0010065.1_g000009 Rmu_sc0010263.1_g000003 Rmu_sc0010632.1_g000004 Rmu_sc0011050.1_g000004 Rmu_sc0011563.1_g000005 Rmu_sc0011854.1_g000001 Rmu_sc0012216.1_g000003 Rmu_sc0012689.1_g000002 Rmu_sc0012945.1_g000001 Rmu_sc0013071.1_g000003 Rmu_sc0013305.1_g000008 Rmu_sc0013698.1_g000009 Rmu_sc0013772.1_g000007 Rmu_sc0013874.1_g000001 Rmu_sc0014217.1_g000001 Rmu_sc0014478.1_g000019 Rmu_sc0014724.1_g000002 Rmu_sc0016563.1_g000002 Rmu_sc0016563.1_g000003 Rmu_sc0017108.1_g000001 Rmu_sc0017408.1_g000002 Rmu_sc0020248.1_g000001 Rmu_sc0021416.1_g000005 Rmu_sc0022221.1_g000003 Rmu_sc0022402.1_g000002 Rmu_sc0023172.1_g000001 Rmu_sc0024783.1_g000001 Rmu_sc0028152.1_g000001 Rmu_sc0028541.1_g000001 Rmu_sc0029935.1_g000001 Rmu_sc0030041.1_g000001 Rmu_sc0035924.1_g000001 Rmu_sc0038113.1_g000002 Rmu_sc0040377.1_g000001 Rmu_ssc0000041.1_g000024 Rmu_ssc0000197.1_g000066 Rmu_ssc0000213.1_g000082 Rmu_ssc0000215.1_g000018
rosa_roxburghii Rroxscaffold_152G00434500 Rroxscaffold_3G00223490 Rroxscaffold_3G00226440 Rroxscaffold_3G00237960 Rroxscaffold_6G00394150
rosa_samantha Rh5CG379600 Rh5CG449200 Rh6DG448500
rosa_wichuraiana Rw0G004320 Rw1G010800 Rw2G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 311
AciI CCGC 4 cut(s) 32, 151, 315, 385
AclWI GGATC 1 cut(s) 140
AdeI CACNNNGTG 2 cut(s) 164, 344
AfiI CCNNNNNNNGG 3 cut(s) 301, 302, 303
AgsI TTSAA 1 cut(s) 197
AluBI AGCT 1 cut(s) 266
AluI AGCT 1 cut(s) 266
Alw21I GWGCWC 2 cut(s) 256, 546
Alw26I GTCTC 2 cut(s) 44, 141
AlwI GGATC 1 cut(s) 140
AoxI GGCC 2 cut(s) 124, 389
ApeKI GCWGC 1 cut(s) 266
AsuHPI GGTGA 1 cut(s) 404
BanI GGYRCC 1 cut(s) 311
BbsI GAAGAC 1 cut(s) 49
Bbv12I GWGCWC 2 cut(s) 256, 546
BbvI GCAGC 1 cut(s) 253
BccI CCATC 1 cut(s) 472
BcoDI GTCTC 2 cut(s) 44, 141
BfaI CTAG 2 cut(s) 24, 249
BglII AGATCT 1 cut(s) 485
BisI GCNGC 2 cut(s) 267, 315
BlsI GCNGC 2 cut(s) 268, 316
BmiI GGNNCC 2 cut(s) 313, 369
BmsI GCATC 2 cut(s) 204, 211
BpiI GAAGAC 1 cut(s) 49
BpuEI CTTGAG 1 cut(s) 513
BsaJI CCNNGG 2 cut(s) 95, 416
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 301, 302, 303
Bse1I ACTGG 1 cut(s) 61
BseDI CCNNGG 2 cut(s) 95, 416
BseLI CCNNNNNNNGG 3 cut(s) 301, 302, 303
BseNI ACTGG 1 cut(s) 61
BseXI GCAGC 1 cut(s) 253
BshFI GGCC 2 cut(s) 126, 391
BshNI GGYRCC 1 cut(s) 311
BsiHKAI GWGCWC 2 cut(s) 256, 546
BslFI GGGAC 1 cut(s) 278
BslI CCNNNNNNNGG 3 cut(s) 301, 302, 303
BsmAI GTCTC 2 cut(s) 44, 141
BsmFI GGGAC 1 cut(s) 278
BsnI GGCC 2 cut(s) 126, 391
Bsp1286I GDGCHC 2 cut(s) 256, 546
Bsp143I GATC 4 cut(s) 10, 145, 190, 485
BspACI CCGC 4 cut(s) 32, 151, 315, 385
BspANI GGCC 2 cut(s) 126, 391
BspLI GGNNCC 2 cut(s) 313, 369
BspPI GGATC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 311
BsrI ACTGG 1 cut(s) 61
BssECI CCNNGG 2 cut(s) 95, 416
BssMI GATC 4 cut(s) 10, 145, 190, 485
BssT1I CCWWGG 2 cut(s) 95, 416
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 2 cut(s) 374, 419
BstC8I GCNNGC 1 cut(s) 279
BstDEI CTNAG 1 cut(s) 341
BstKTI GATC 4 cut(s) 13, 148, 193, 488
BstMAI GTCTC 2 cut(s) 44, 141
BstMBI GATC 4 cut(s) 10, 145, 190, 485
BstMWI GCNNNNNNNGC 1 cut(s) 29
BstV1I GCAGC 1 cut(s) 253
BstV2I GAAGAC 1 cut(s) 49
BstX2I RGATCY 1 cut(s) 485
BstYI RGATCY 1 cut(s) 485
BsuRI GGCC 2 cut(s) 126, 391
BtsI GCAGTG 1 cut(s) 327
BtsIMutI CAGTG 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 279
CviAII CATG 2 cut(s) 107, 308
CviJI RGCY 4 cut(s) 126, 266, 368, 391
CviKI_1 RGCY 4 cut(s) 126, 266, 368, 391
DdeI CTNAG 1 cut(s) 341
DpnI GATC 4 cut(s) 12, 147, 192, 487
DpnII GATC 4 cut(s) 10, 145, 190, 485
DraIII CACNNNGTG 2 cut(s) 164, 344
Eam1104I CTCTTC 2 cut(s) 374, 419
EarI CTCTTC 2 cut(s) 374, 419
EciI GGCGGA 1 cut(s) 400
Eco130I CCWWGG 2 cut(s) 95, 416
Eco32I GATATC 2 cut(s) 235, 553
EcoRV GATATC 2 cut(s) 235, 553
EcoT14I CCWWGG 2 cut(s) 95, 416
ErhI CCWWGG 2 cut(s) 95, 416
FaeI CATG 2 cut(s) 110, 311
FaqI GGGAC 1 cut(s) 278
FatI CATG 2 cut(s) 106, 307
FauI CCCGC 1 cut(s) 158
Fnu4HI GCNGC 2 cut(s) 267, 315
Fsp4HI GCNGC 2 cut(s) 267, 315
FspBI CTAG 2 cut(s) 24, 249
GluI GCNGC 2 cut(s) 267, 315
HaeIII GGCC 2 cut(s) 126, 391
Hin1II CATG 2 cut(s) 110, 311
HincII GTYRAC 1 cut(s) 581
HindII GTYRAC 1 cut(s) 581
HinfI GANTC 1 cut(s) 209
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 228, 581
Hpy188III TCNNGA 1 cut(s) 530
Hpy8I GTNNAC 2 cut(s) 228, 581
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4V TGCA 5 cut(s) 269, 281, 332, 515, 572
HpyF10VI GCNNNNNNNGC 1 cut(s) 29
HpyF3I CTNAG 1 cut(s) 341
Hsp92II CATG 2 cut(s) 110, 311
Kzo9I GATC 4 cut(s) 10, 145, 190, 485
LmnI GCTCC 1 cut(s) 373
LpnPI CCDG 3 cut(s) 74, 231, 291
Lsp1109I GCAGC 1 cut(s) 253
LweI GCATC 2 cut(s) 204, 211
MaeI CTAG 2 cut(s) 24, 249
MaeIII GTNAC 2 cut(s) 38, 244
MalI GATC 4 cut(s) 12, 147, 192, 487
MboI GATC 4 cut(s) 10, 145, 190, 485
MboII GAAGA 7 cut(s) 49, 185, 391, 433, 436, 445, 480
MflI RGATCY 1 cut(s) 485
MhlI GDGCHC 2 cut(s) 256, 546
MluCI AATT 1 cut(s) 110
MnlI CCTC 7 cut(s) 137, 317, 341, 352, 375, 381, 420
MslI CAYNNNNRTG 1 cut(s) 501
MspA1I CMGCKG 1 cut(s) 266
MwoI GCNNNNNNNGC 1 cut(s) 29
NdeII GATC 4 cut(s) 10, 145, 190, 485
NlaIII CATG 2 cut(s) 110, 311
NlaIV GGNNCC 2 cut(s) 313, 369
NmuCI GTSAC 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 552
PfeI GAWTC 1 cut(s) 209
PkrI GCNGC 2 cut(s) 268, 316
PspN4I GGNNCC 2 cut(s) 313, 369
PsuI RGATCY 1 cut(s) 485
PvuII CAGCTG 1 cut(s) 266
RseI CAYNNNNRTG 1 cut(s) 501
SatI GCNGC 2 cut(s) 267, 315
Sau3AI GATC 4 cut(s) 10, 145, 190, 485
SduI GDGCHC 2 cut(s) 256, 546
SetI ASST 4 cut(s) 268, 287, 352, 363
SfaNI GCATC 2 cut(s) 204, 211
SmiMI CAYNNNNRTG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
Sse9I AATT 1 cut(s) 110
SsiI CCGC 4 cut(s) 32, 151, 315, 385
SspMI CTAG 2 cut(s) 24, 249
StyI CCWWGG 2 cut(s) 95, 416
TaaI ACNGT 1 cut(s) 55
TaqI TCGA 4 cut(s) 189, 212, 401, 555
TasI AATT 1 cut(s) 110
TauI GCSGC 1 cut(s) 317
TfiI GAWTC 1 cut(s) 209
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 244
TseI GCWGC 1 cut(s) 266
Tsp45I GTSAC 1 cut(s) 244
TspDTI ATGAA 1 cut(s) 123
TspRI CASTG 1 cut(s) 334
XspI CTAG 2 cut(s) 24, 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.