RchiOBHm_Chr2g0163801

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0000161
Physical Location & Seq
Forward (+)
79120563 .. 79123510
2948 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ53193

Sequence Viewer

Length: 2838 bp
ATGATTGTTCCTCCAAATAGCTACAATGCCATAGAAGGAGATGTAGTCACACATTTTGCTTCTAATAGCGCTACAGACGCTCAGGCCCAACCAAAATCCTCATTGGTTGCTTCTAAGGCCCCTCGCTCATTTTGCAAAGCCTGTTCCTTCGGGAAATTAGGACCGAGACCGTCCTACGCAAAGGATCCTAAAATACTCATTCCGTTCTTACAGAGAATCCAAGGGGATATCTGTGGACCAATTCAACCATCATGCGGACCTTTCAAATACTTTATGGTATTGGTTGATGCGTCGACACGCTGGTCACATGTCGCGCTGTTGTCCACTCGAAATGCTGCTTATGCTAAACTCCTCGCCCAGATTATCCGTCTACGGGCTCACTACCCTGACCATCCAATTAAGTCAATTCGACTTGATAATGCTGGGGAGTTTACATCGAAAACATTCGATGACTATTGCATGTCACTGGGGATTGATGTAGAACATCCAGTTCCCCATGTTCATACCCAAAATGGTCTCGCAGAAGCTGCTATCAAACGACTACAAATGATAGCACGGACATTGGTAATGCACACTAATCTCCCTGTTTCTGCTTGGGGATATGCAATATTACATGCAGCGACGCTAATTCGTCTACGACCCACTGCAACCCAATCTTACTCTGCGTTACAGCTAGTGACTGGGTACGAGCCTAATATCTCGCACTTACGCATTTTTGGGTGTACTATTTATGTGCCTATTACGCCGCCACAGCGTACTAAGATGGGTCCACAACGACGAATAAGCATTTATGTTGGATATGAATCTCCAACTATCGTCCGCTATCTTGAACCCTTGACAGGCGATCTCTTTACCGCTAGATTTGCGGATTGTCACTTTGATGAGACAGTCTTCCCATCATTAGGGGGAGATAAGAACACAAATGTTCAACAGGAACGACAGGAATTGTCGTGGTCTGTCCCCACTATGTCTCATCTCGATCCCCATACCGCACAGTCCGAACTTGAAGTGCGAAGAATAATCGAGCTCCAGAACGTAGCAGACACTCTGCCTGATGCGTTTTCTGATATTGCTAAAGTGACGAGATCACACATACCTGCTGCAAACGTGCCTGCAAGGATTGATGTCCCGAATAATGGACATAACGCCACTCCTTTAACGCTAGGAGATGGCACCATTGCTCAACATGGCAATAATGTGGCATCTATGGCTGTAGGTCCCGCAAGGAAGCGCGGTAGACCGATTGGTTCGAAGGATACTCGCCCTAGAAAAAGAGCGAATGAGGCACAAATAAATCCTTTGATCATCGATACTCAAAATCCGTCCCATGAGAATGTTCCGGATTATGGTTATGTCCAAGAGACATCATTGGGGGACGCCTCAGTGTCAGAACCTATCCATGAGAACATAGAGATCTCTGTAAATTACACTAGTGTACATGGGACGTGGGAAAGAAACTCCATCATCATTGATGATGTATTCGCGTATTCAGTGGCGCGTGAGATTATTGAGACCGATGACATCGAACCACGCTCCGTTGATGAATGTCAACGTAGAGCTGATTGGCCAAAATGGAAAGATGCGATCCAGGCAGAACTTGATTCTCTAACGAAAAGAAAGGTATTTGGCAAAATTGTACCAATACCACCCAACACCAAACCAGTTGGTCACAAATGGGTATTCGTTAGAAAGCGTAATGAGAAAAACGAGATTGTAAGATACAAAGCTCGTCTCGTGGCGCAAGGCTTCTCACAACGCCCTGGAATCGACTACGAGGAGACCTACTCTCCCGTAATGGACGTTATTACGTTCCGCTACCTTGTCAGTTTGGTAGTTTCCGAAAAACTGAACATGCAGCTGATGGATGTGGTTACTGCATATCTATATGGGGATCTAGATACGGAGATATACATGAATATTCCAGATGGAATTCAACTACCCAAATCAAGTGGCTCTAATCCACGGAGCGCGTTTGCAATTAGATTGAAACGCTCACTATATGGATTAAAACAATCCGGACGGATGTGGTATAACCGTCTAAGTGACTACTTGATTGGTAAGGGATATGTCAATAACGAACTATGCCCATGTGTGTTCATAAAAAGGACAAGTTCCGGATTTTCAATAGTAGCGGTTTATGTCGATGACATGAACATAATTGGCACCCTTAAAGAGTTAAGGGAAACCGCTGAACACTTGAAATCCGAGTTTGAGATGAAAGATCTTGGGAAAACACGGTTTTGCCTCGGTTTAGAACTTGAGCACCGTAGAGATGGTATCCTGATTCATCAATCAGCTTATACCCAAAAGATGCTTAGGCGTTTCAACACTGACAAAATCAAGCCTTCAAGCACCCCAATGGTCGTCCGTAGTCTTGATCCAAAGAAGGATCCATTTCGTCCAAAAGATGACGATGAAGAAGTGCTAGAGGCAGAAGTGCCCTACCTAAGTGCAATAGGTGCATTATTGTACTTAGCACAATGCACGAGACCAGACATCTCATTCGCTGTGAACTTGCTAGCTAGATATAGCTCTGCGCCAACACGACGCCATTGGACTGGTGTTAAAGATATCTTTCGATACCTAAGTAGTACGATCGATATGGGCTTGTTCTATCCCTACAGAAAGAAGATGGATTCGGACCCATCAAGTGTCAGGAACGCCACGCATAGTGGATTGCGTTCCCTCTCCCCATCCCAAAACGATATAAGTGTTTTGGAAGGTTTTGCTGATGCTGGGTACCTCTCTGACCCACACAAAGGTCGCTCCCAAACTGACCCTAGTCGCTACCTCTTCAAATCATGCAGAGATTATTGCTCTTCACGAAGCAGTTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

945

Amino Acids

106.08

Weight (kDa)

8.82

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
rve PF00665 71 - 162 2.5e-09 Integrase core domain
RVT_2 PF07727 544 - 788 3.5e-58 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000161)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36192 FvH4_3g41702 FvH4_4g13933 FvH4_6g39331 FvH4_6g44542
malus_domestica MD17G1101700.v1.1
pyrus_communis pycom01g01810 pycom01g03340 pycom05g22610 pycom13g03220 pycom13g23770 pycom13g26870 pycom17g17400 pycom17g17410 pycom17g17890
rosa_chinensis RchiOBHm_Chr2g0129881 RchiOBHm_Chr2g0163801 RchiOBHm_Chr4g0392871 RchiOBHm_Chr7g0218471
rosa_multiflora Rmu_co8005914.1_g000001 Rmu_co8214666.1_g000002 Rmu_co8253889.1_g000001 Rmu_co8429419.1_g000001 Rmu_sc0000031.1_g000049 Rmu_sc0000091.1_g000004 Rmu_sc0000103.1_g000050 Rmu_sc0000147.1_g000047 Rmu_sc0000185.1_g000026 Rmu_sc0000235.1_g000056 Rmu_sc0000247.1_g000002 Rmu_sc0000310.1_g000010 Rmu_sc0000353.1_g000034 Rmu_sc0000353.1_g000035 Rmu_sc0000367.1_g000046 Rmu_sc0000420.1_g000034 Rmu_sc0000461.1_g000022 Rmu_sc0000544.1_g000007 Rmu_sc0000544.1_g000009 Rmu_sc0000607.1_g000032 Rmu_sc0000647.1_g000011 Rmu_sc0000675.1_g000049 Rmu_sc0000680.1_g000015 Rmu_sc0000795.1_g000112 Rmu_sc0000851.1_g000013 Rmu_sc0000868.1_g000019 Rmu_sc0000869.1_g000009 Rmu_sc0000898.1_g000061 Rmu_sc0000990.1_g000006 Rmu_sc0001009.1_g000006 Rmu_sc0001051.1_g000034 Rmu_sc0001067.1_g000005 Rmu_sc0001124.1_g000020 Rmu_sc0001142.1_g000016 Rmu_sc0001231.1_g000015 Rmu_sc0001235.1_g000013 Rmu_sc0001336.1_g000036 Rmu_sc0001552.1_g000033 Rmu_sc0001604.1_g000003 Rmu_sc0001605.1_g000009 Rmu_sc0001605.1_g000011 Rmu_sc0001668.1_g000025 Rmu_sc0001698.1_g000009 Rmu_sc0001721.1_g000010 Rmu_sc0001735.1_g000004 Rmu_sc0001832.1_g000017 Rmu_sc0001844.1_g000024 Rmu_sc0001880.1_g000026 Rmu_sc0001896.1_g000018 Rmu_sc0001918.1_g000011 Rmu_sc0002072.1_g000022 Rmu_sc0002109.1_g000003 Rmu_sc0002142.1_g000017 Rmu_sc0002166.1_g000001 Rmu_sc0002207.1_g000015 Rmu_sc0002346.1_g000001 Rmu_sc0002460.1_g000009 Rmu_sc0002460.1_g000043 Rmu_sc0002460.1_g000071 Rmu_sc0002672.1_g000001 Rmu_sc0002699.1_g000019 Rmu_sc0002736.1_g000007 Rmu_sc0002773.1_g000003 Rmu_sc0002893.1_g000006 Rmu_sc0002968.1_g000014 Rmu_sc0002968.1_g000015 Rmu_sc0003032.1_g000020 Rmu_sc0003044.1_g000024 Rmu_sc0003200.1_g000007 Rmu_sc0003261.1_g000031 Rmu_sc0003347.1_g000014 Rmu_sc0003385.1_g000019 Rmu_sc0003385.1_g000020 Rmu_sc0003419.1_g000017 Rmu_sc0003605.1_g000029 Rmu_sc0003679.1_g000010 Rmu_sc0003708.1_g000002 Rmu_sc0003737.1_g000033 Rmu_sc0003981.1_g000021 Rmu_sc0004101.1_g000009 Rmu_sc0004119.1_g000009 Rmu_sc0004248.1_g000021 Rmu_sc0004362.1_g000013 Rmu_sc0004520.1_g000020 Rmu_sc0004741.1_g000017 Rmu_sc0004771.1_g000007 Rmu_sc0004835.1_g000027 Rmu_sc0004969.1_g000010 Rmu_sc0005037.1_g000002 Rmu_sc0005272.1_g000006 Rmu_sc0005584.1_g000017 Rmu_sc0005652.1_g000005 Rmu_sc0005670.1_g000010 Rmu_sc0005781.1_g000035 Rmu_sc0005816.1_g000026 Rmu_sc0005852.1_g000003 Rmu_sc0006087.1_g000008 Rmu_sc0006152.1_g000005 Rmu_sc0006160.1_g000001 Rmu_sc0006313.1_g000014 Rmu_sc0006347.1_g000001 Rmu_sc0006583.1_g000024 Rmu_sc0006711.1_g000018 Rmu_sc0006952.1_g000003 Rmu_sc0006976.1_g000006 Rmu_sc0007115.1_g000006 Rmu_sc0007490.1_g000028 Rmu_sc0007740.1_g000003 Rmu_sc0007779.1_g000001 Rmu_sc0007907.1_g000003 Rmu_sc0007967.1_g000003 Rmu_sc0008456.1_g000006 Rmu_sc0008578.1_g000003 Rmu_sc0008642.1_g000001 Rmu_sc0008848.1_g000001 Rmu_sc0010065.1_g000009 Rmu_sc0010263.1_g000003 Rmu_sc0010632.1_g000004 Rmu_sc0011050.1_g000004 Rmu_sc0011563.1_g000005 Rmu_sc0011854.1_g000001 Rmu_sc0012216.1_g000003 Rmu_sc0012689.1_g000002 Rmu_sc0012945.1_g000001 Rmu_sc0013071.1_g000003 Rmu_sc0013305.1_g000008 Rmu_sc0013698.1_g000009 Rmu_sc0013772.1_g000007 Rmu_sc0013874.1_g000001 Rmu_sc0014217.1_g000001 Rmu_sc0014478.1_g000019 Rmu_sc0014724.1_g000002 Rmu_sc0016563.1_g000002 Rmu_sc0016563.1_g000003 Rmu_sc0017108.1_g000001 Rmu_sc0017408.1_g000002 Rmu_sc0020248.1_g000001 Rmu_sc0021416.1_g000005 Rmu_sc0022221.1_g000003 Rmu_sc0022402.1_g000002 Rmu_sc0023172.1_g000001 Rmu_sc0024783.1_g000001 Rmu_sc0028152.1_g000001 Rmu_sc0028541.1_g000001 Rmu_sc0029935.1_g000001 Rmu_sc0030041.1_g000001 Rmu_sc0035924.1_g000001 Rmu_sc0038113.1_g000002 Rmu_sc0040377.1_g000001 Rmu_ssc0000041.1_g000024 Rmu_ssc0000197.1_g000066 Rmu_ssc0000213.1_g000082 Rmu_ssc0000215.1_g000018
rosa_roxburghii Rroxscaffold_152G00434500 Rroxscaffold_3G00223490 Rroxscaffold_3G00226440 Rroxscaffold_3G00237960 Rroxscaffold_6G00394150
rosa_samantha Rh5CG379600 Rh5CG449200 Rh6DG448500
rosa_wichuraiana Rw0G004320 Rw1G010800 Rw2G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 301
Acc36I ACCTGC 1 cut(s) 1105
Acc65I GGTACC 1 cut(s) 2739
AccB1I GGYRCC 3 cut(s) 1172, 2162, 2739
AccI GTMKAC 4 cut(s) 293, 370, 634, 1237
AccII CGCG 5 cut(s) 314, 1233, 1484, 1498, 1970
AccIII TCCGGA 3 cut(s) 1339, 2015, 2114
AclWI GGATC 8 cut(s) 179, 192, 974, 1579, 1899, 2372, 2384, 2397
AcoI YGGCCR 1 cut(s) 1565
AcsI RAATTY 1 cut(s) 1929
AcyI GRCGYC 2 cut(s) 1377, 2548
AfaI GTAC 8 cut(s) 686, 724, 757, 1437, 1638, 2471, 2593, 2741
AfeI AGCGCT 1 cut(s) 70
AfiI CCNNNNNNNGG 7 cut(s) 254, 373, 839, 902, 1136, 1346, 2759
AflIII ACRYGT 1 cut(s) 307
AhlI ACTAGT 1 cut(s) 1430
AjiI CACGTC 1 cut(s) 1446
AjnI CCWGG 2 cut(s) 1587, 1759
Alw21I GWGCWC 2 cut(s) 1029, 2265
Alw26I GTCTC 9 cut(s) 160, 521, 878, 975, 1355, 1505, 1736, 1772, 2482
AlwI GGATC 8 cut(s) 179, 192, 974, 1579, 1899, 2372, 2384, 2397
AlwNI CAGNNNCTG 1 cut(s) 527
Aor13HI TCCGGA 3 cut(s) 1339, 2015, 2114
Aor51HI AGCGCT 1 cut(s) 70
AoxI GGCC 3 cut(s) 84, 117, 1565
ApeKI GCWGC 5 cut(s) 335, 527, 617, 1100, 1855
ApoI RAATTY 1 cut(s) 1929
ArsI GACNNNNNNTTYG 2 cut(s) 2486, 2518
Asp700I GAANNNNTTC 2 cut(s) 443, 2830
Asp718I GGTACC 1 cut(s) 2739
AspLEI GCGC 7 cut(s) 71, 316, 1233, 1498, 1741, 1970, 2539
AspS9I GGNCC 8 cut(s) 85, 118, 161, 236, 257, 767, 1217, 2641
AsuII TTCGAA 1 cut(s) 1250
AsuNHI GCTAGC 1 cut(s) 2518
AvaII GGWCC 6 cut(s) 161, 236, 257, 767, 1217, 2641
BaeGI GKGCMC 1 cut(s) 2442
BaeI ACNNNNGTAYC 2 cut(s) 2572, 2605
BalI TGGCCA 1 cut(s) 1567
BamHI GGATCC 2 cut(s) 184, 2389
BanI GGYRCC 3 cut(s) 1172, 2162, 2739
BanII GRGCYC 2 cut(s) 379, 1029
BauI CACGAG 2 cut(s) 1733, 2485
BbsI GAAGAC 1 cut(s) 883
Bbv12I GWGCWC 2 cut(s) 1029, 2265
BbvI GCAGC 5 cut(s) 322, 514, 629, 1087, 1867
BciT130I CCWGG 2 cut(s) 1589, 1761
BciVI GTATCC 2 cut(s) 1249, 2288
BclI TGATCA 1 cut(s) 1302
BcoDI GTCTC 9 cut(s) 160, 521, 878, 975, 1355, 1505, 1736, 1772, 2482
BcuI ACTAGT 1 cut(s) 1430
BfmI CTRYAG 3 cut(s) 72, 1212, 2620
BfoI RGCGCY 1 cut(s) 72
BfuAI ACCTGC 1 cut(s) 1105
BfuI GTATCC 2 cut(s) 1249, 2288
BglII AGATCT 2 cut(s) 1413, 2221
BisI GCNGC 6 cut(s) 336, 528, 618, 746, 1101, 1856
BlsI GCNGC 6 cut(s) 337, 529, 619, 747, 1102, 1857
Bme1390I CCNGG 2 cut(s) 1589, 1761
Bme18I GGWCC 6 cut(s) 161, 236, 257, 767, 1217, 2641
BmgBI CACGTC 1 cut(s) 1446
BmgT120I GGNCC 8 cut(s) 85, 118, 161, 236, 257, 767, 1217, 2641
BmiI GGNNCC 9 cut(s) 120, 186, 768, 1174, 1219, 2164, 2391, 2643, 2741
BmrFI CCNGG 2 cut(s) 1589, 1761
BmrI ACTGGG 2 cut(s) 476, 690
BmsI GCATC 6 cut(s) 277, 1045, 1211, 1570, 2301, 2722
BmtI GCTAGC 1 cut(s) 2522
BmuI ACTGGG 2 cut(s) 476, 690
BoxI GACNNNNGTC 1 cut(s) 2781
BpiI GAAGAC 1 cut(s) 883
BplI GAGNNNNNCTC 2 cut(s) 1769, 1801
BpmI CTGGAG 1 cut(s) 1013
Bpu10I CCTNAGC 2 cut(s) 81, 2315
Bpu14I TTCGAA 1 cut(s) 1250
BpuEI CTTGAG 1 cut(s) 2279
Bsa29I ATCGAT 2 cut(s) 1308, 2598
BsaBI GATNNNNATC 1 cut(s) 802
BsaHI GRCGYC 2 cut(s) 1377, 2548
BsaI GGTCTC 5 cut(s) 160, 521, 1505, 1772, 2482
BsaJI CCNNGG 4 cut(s) 220, 1759, 1961, 2245
BsaWI WCCGGW 3 cut(s) 1339, 2015, 2114
BsaXI ACNNNNNCTCC 4 cut(s) 30, 60, 1771, 1801
Bsc4I CCNNNNNNNGG 7 cut(s) 254, 373, 839, 902, 1136, 1346, 2759
Bse1I ACTGG 5 cut(s) 471, 488, 685, 1661, 2563
Bse3DI GCAATG 1 cut(s) 1176
Bse8I GATNNNNATC 1 cut(s) 802
BseAI TCCGGA 3 cut(s) 1339, 2015, 2114
BseBI CCWGG 2 cut(s) 1589, 1761
BseCI ATCGAT 2 cut(s) 1308, 2598
BseDI CCNNGG 4 cut(s) 220, 1759, 1961, 2245
BseGI GGATG 5 cut(s) 391, 484, 1870, 2028, 2693
BseJI GATNNNNATC 1 cut(s) 802
BseLI CCNNNNNNNGG 7 cut(s) 254, 373, 839, 902, 1136, 1346, 2759
BseMI GCAATG 1 cut(s) 1176
BseMII CTCAG 2 cut(s) 95, 1395
BseNI ACTGG 5 cut(s) 471, 488, 685, 1661, 2563
BseRI GAGGAG 2 cut(s) 341, 1790
BseSI GKGCMC 1 cut(s) 2442
BseXI GCAGC 5 cut(s) 322, 514, 629, 1087, 1867
BseYI CCCAGC 2 cut(s) 422, 2735
Bsh1236I CGCG 5 cut(s) 314, 1233, 1484, 1498, 1970
Bsh1285I CGRYCG 1 cut(s) 2598
BshFI GGCC 3 cut(s) 86, 119, 1567
BshNI GGYRCC 3 cut(s) 1172, 2162, 2739
BshVI ATCGAT 2 cut(s) 1308, 2598
BsiEI CGRYCG 1 cut(s) 2598
BsiHKAI GWGCWC 2 cut(s) 1029, 2265
BsiSI CCGG 3 cut(s) 1340, 2016, 2115
BslFI GGGAC 6 cut(s) 944, 1112, 1203, 1309, 1388, 1456
BslI CCNNNNNNNGG 7 cut(s) 254, 373, 839, 902, 1136, 1346, 2759
BsmAI GTCTC 9 cut(s) 160, 521, 878, 975, 1355, 1505, 1736, 1772, 2482
BsmBI CGTCTC 1 cut(s) 1736
BsmFI GGGAC 6 cut(s) 944, 1112, 1203, 1309, 1388, 1456
BsnI GGCC 3 cut(s) 86, 119, 1567
Bso31I GGTCTC 5 cut(s) 160, 521, 1505, 1772, 2482
Bsp119I TTCGAA 1 cut(s) 1250
Bsp1286I GDGCHC 4 cut(s) 379, 1029, 2265, 2442
Bsp13I TCCGGA 3 cut(s) 1339, 2015, 2114
Bsp1407I TGTACA 1 cut(s) 1435
BspANI GGCC 3 cut(s) 86, 119, 1567
BspCNI CTCAG 2 cut(s) 94, 1394
BspDI ATCGAT 2 cut(s) 1308, 2598
BspEI TCCGGA 3 cut(s) 1339, 2015, 2114
BspFNI CGCG 5 cut(s) 314, 1233, 1484, 1498, 1970
BspLI GGNNCC 9 cut(s) 120, 186, 768, 1174, 1219, 2164, 2391, 2643, 2741
BspMI ACCTGC 1 cut(s) 1105
BspOI GCTAGC 1 cut(s) 2522
BspPI GGATC 8 cut(s) 179, 192, 974, 1579, 1899, 2372, 2384, 2397
BspQI GCTCTTC 1 cut(s) 2824
BspT104I TTCGAA 1 cut(s) 1250
BspT107I GGYRCC 3 cut(s) 1172, 2162, 2739
BspTNI GGTCTC 5 cut(s) 160, 521, 1505, 1772, 2482
BsrDI GCAATG 1 cut(s) 1176
BsrGI TGTACA 1 cut(s) 1435
BsrI ACTGG 5 cut(s) 471, 488, 685, 1661, 2563
BssECI CCNNGG 4 cut(s) 220, 1759, 1961, 2245
BssNI GRCGYC 2 cut(s) 1377, 2548
BssSI CACGAG 2 cut(s) 1733, 2485
BssT1I CCWWGG 1 cut(s) 220
Bst2BI CACGAG 2 cut(s) 1733, 2485
Bst2UI CCWGG 2 cut(s) 1589, 1761
Bst4CI ACNGT 6 cut(s) 171, 889, 996, 2036, 2238, 2267
Bst6I CTCTTC 2 cut(s) 2798, 2824
BstACI GRCGYC 2 cut(s) 1377, 2548
BstAPI GCANNNNNTGC 2 cut(s) 527, 2459
BstAUI TGTACA 1 cut(s) 1435
BstBI TTCGAA 1 cut(s) 1250
BstC8I GCNNGC 2 cut(s) 1113, 2520
BstDEI CTNAG 9 cut(s) 81, 114, 759, 1381, 2039, 2315, 2447, 2473, 2585
BstDSI CCRYGG 1 cut(s) 1961
BstF5I GGATG 5 cut(s) 391, 484, 1870, 2028, 2693
BstFNI CGCG 5 cut(s) 314, 1233, 1484, 1498, 1970
BstH2I RGCGCY 1 cut(s) 72
BstHHI GCGC 7 cut(s) 71, 316, 1233, 1498, 1741, 1970, 2539
BstMAI GTCTC 9 cut(s) 160, 521, 878, 975, 1355, 1505, 1736, 1772, 2482
BstMCI CGRYCG 1 cut(s) 2598
BstNI CCWGG 2 cut(s) 1589, 1761
BstNSI RCATGY 4 cut(s) 311, 463, 617, 1855
BstPAI GACNNNNGTC 1 cut(s) 2781
BstSCI CCNGG 2 cut(s) 1587, 1759
BstSFI CTRYAG 3 cut(s) 72, 1212, 2620
BstSLI GKGCMC 1 cut(s) 2442
BstUI CGCG 5 cut(s) 314, 1233, 1484, 1498, 1970
BstV1I GCAGC 5 cut(s) 322, 514, 629, 1087, 1867
BstV2I GAAGAC 1 cut(s) 883
BstX2I RGATCY 5 cut(s) 184, 1413, 1891, 2221, 2389
BstXI CCANNNNNNTGG 1 cut(s) 2558
BstYI RGATCY 5 cut(s) 184, 1413, 1891, 2221, 2389
Bsu15I ATCGAT 2 cut(s) 1308, 2598
BsuI GTATCC 2 cut(s) 1249, 2288
BsuRI GGCC 3 cut(s) 86, 119, 1567
BsuTUI ATCGAT 2 cut(s) 1308, 2598
BtgI CCRYGG 1 cut(s) 1961
BtrI CACGTC 1 cut(s) 1446
BtsCI GGATG 5 cut(s) 391, 484, 1870, 2028, 2693
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 5 cut(s) 464, 642, 1389, 1497, 2328
BveI ACCTGC 1 cut(s) 1105
Cac8I GCNNGC 2 cut(s) 1113, 2520
CaiI CAGNNNCTG 1 cut(s) 527
CfoI GCGC 7 cut(s) 71, 316, 1233, 1498, 1741, 1970, 2539
Cfr13I GGNCC 8 cut(s) 85, 118, 161, 236, 257, 767, 1217, 2641
ClaI ATCGAT 2 cut(s) 1308, 2598
CseI GACGC 5 cut(s) 86, 279, 631, 1385, 2556
Csp6I GTAC 8 cut(s) 685, 723, 756, 1436, 1637, 2470, 2592, 2740
CspCI CAANNNNNGTGG 2 cut(s) 1930, 1965
CviQI GTAC 8 cut(s) 685, 723, 756, 1436, 1637, 2470, 2592, 2740
DdeI CTNAG 9 cut(s) 81, 114, 759, 1381, 2039, 2315, 2447, 2473, 2585
DrdI GACNNNNNNGTC 1 cut(s) 301
DseDI GACNNNNNNGTC 1 cut(s) 301
EaeI YGGCCR 1 cut(s) 1565
Eam1104I CTCTTC 2 cut(s) 2798, 2824
EarI CTCTTC 2 cut(s) 2798, 2824
Ecl136II GAGCTC 1 cut(s) 1027
Eco130I CCWWGG 1 cut(s) 220
Eco24I GRGCYC 2 cut(s) 379, 1029
Eco31I GGTCTC 5 cut(s) 160, 521, 1505, 1772, 2482
Eco32I GATATC 2 cut(s) 229, 2572
Eco47I GGWCC 6 cut(s) 161, 236, 257, 767, 1217, 2641
Eco47III AGCGCT 1 cut(s) 70
Eco53kI GAGCTC 1 cut(s) 1027
EcoICRI GAGCTC 1 cut(s) 1027
EcoO109I RGGNCCY 2 cut(s) 118, 1217
EcoRI GAATTC 1 cut(s) 1929
EcoRII CCWGG 2 cut(s) 1587, 1759
EcoRV GATATC 2 cut(s) 229, 2572
EcoT14I CCWWGG 1 cut(s) 220
EcoT38I GRGCYC 2 cut(s) 379, 1029
ErhI CCWWGG 1 cut(s) 220
Esp3I CGTCTC 1 cut(s) 1736
FaqI GGGAC 6 cut(s) 944, 1112, 1203, 1309, 1388, 1456
FauI CCCGC 1 cut(s) 1228
FbaI TGATCA 1 cut(s) 1302
FblI GTMKAC 4 cut(s) 293, 370, 634, 1237
Fnu4HI GCNGC 6 cut(s) 336, 528, 618, 746, 1101, 1856
FokI GGATG 5 cut(s) 378, 471, 1877, 2035, 2680
FriOI GRGCYC 2 cut(s) 379, 1029
Fsp4HI GCNGC 6 cut(s) 336, 528, 618, 746, 1101, 1856
GlaI GCGC 7 cut(s) 70, 315, 1232, 1497, 1740, 1969, 2538
GluI GCNGC 6 cut(s) 336, 528, 618, 746, 1101, 1856
GsaI CCCAGC 2 cut(s) 426, 2739
GsuI CTGGAG 1 cut(s) 1013
HaeII RGCGCY 1 cut(s) 72
HaeIII GGCC 3 cut(s) 86, 119, 1567
HapII CCGG 3 cut(s) 1340, 2016, 2115
HgaI GACGC 5 cut(s) 86, 279, 631, 1385, 2556
HhaI GCGC 7 cut(s) 71, 316, 1233, 1498, 1741, 1970, 2539
Hin1I GRCGYC 2 cut(s) 1377, 2548
Hin6I GCGC 7 cut(s) 69, 314, 1231, 1496, 1739, 1968, 2537
HinP1I GCGC 7 cut(s) 69, 314, 1231, 1496, 1739, 1968, 2537
HincII GTYRAC 2 cut(s) 294, 1550
HindII GTYRAC 2 cut(s) 294, 1550
HinfI GANTC 6 cut(s) 216, 803, 1601, 1764, 2284, 2636
HpaII CCGG 3 cut(s) 1340, 2016, 2115
Hpy188I TCNGA 7 cut(s) 1000, 1066, 1390, 1840, 2206, 2641, 2749
Hpy99I CGWCG 4 cut(s) 295, 625, 780, 2550
HpyAV CCTTC 6 cut(s) 29, 157, 1246, 2355, 2380, 2714
HpyCH4III ACNGT 6 cut(s) 171, 889, 996, 2036, 2238, 2267
HpyCH4IV ACGT 6 cut(s) 1035, 1107, 1445, 1552, 1800, 1808
HpyF3I CTNAG 9 cut(s) 81, 114, 759, 1381, 2039, 2315, 2447, 2473, 2585
HpySE526I ACGT 6 cut(s) 1035, 1107, 1445, 1552, 1800, 1808
Hsp92I GRCGYC 2 cut(s) 1377, 2548
HspAI GCGC 7 cut(s) 69, 314, 1231, 1496, 1739, 1968, 2537
Kpn2I TCCGGA 3 cut(s) 1339, 2015, 2114
KpnI GGTACC 1 cut(s) 2743
Ksp22I TGATCA 1 cut(s) 1302
LguI GCTCTTC 1 cut(s) 2824
LmnI GCTCC 4 cut(s) 1032, 1538, 1965, 2771
Lsp1109I GCAGC 5 cut(s) 322, 514, 629, 1087, 1867
LweI GCATC 6 cut(s) 277, 1045, 1211, 1570, 2301, 2722
MaeII ACGT 6 cut(s) 1035, 1107, 1445, 1552, 1800, 1808
MboII GAAGA 6 cut(s) 883, 1026, 2429, 2641, 2785, 2811
MflI RGATCY 5 cut(s) 184, 1413, 1891, 2221, 2389
MhlI GDGCHC 4 cut(s) 379, 1029, 2265, 2442
MlsI TGGCCA 1 cut(s) 1567
MluNI TGGCCA 1 cut(s) 1567
MmeI TCCRAC 2 cut(s) 775, 833
Mox20I TGGCCA 1 cut(s) 1567
MroI TCCGGA 3 cut(s) 1339, 2015, 2114
MroXI GAANNNNTTC 2 cut(s) 443, 2830
MscI TGGCCA 1 cut(s) 1567
MseI TTAA 6 cut(s) 399, 1157, 2006, 2169, 2177, 2565
MslI CAYNNNNRTG 3 cut(s) 879, 1332, 2357
Msp20I TGGCCA 1 cut(s) 1567
MspA1I CMGCKG 2 cut(s) 1858, 2189
MspI CCGG 3 cut(s) 1340, 2016, 2115
MspR9I CCNGG 2 cut(s) 1589, 1761
MvaI CCWGG 2 cut(s) 1589, 1761
MvnI CGCG 5 cut(s) 314, 1233, 1484, 1498, 1970
NheI GCTAGC 1 cut(s) 2518
NlaIV GGNNCC 9 cut(s) 120, 186, 768, 1174, 1219, 2164, 2391, 2643, 2741
NmuCI GTSAC 8 cut(s) 46, 303, 462, 676, 872, 1078, 1667, 2042
NspI RCATGY 4 cut(s) 311, 463, 617, 1855
NspV TTCGAA 1 cut(s) 1250
PciI ACATGT 1 cut(s) 307
PciSI GCTCTTC 1 cut(s) 2824
PdmI GAANNNNTTC 2 cut(s) 443, 2830
PfeI GAWTC 6 cut(s) 216, 803, 1601, 1764, 2284, 2636
PkrI GCNGC 6 cut(s) 337, 529, 619, 747, 1102, 1857
Ple19I CGATCG 1 cut(s) 2598
PpuMI RGGWCCY 1 cut(s) 1217
PscI ACATGT 1 cut(s) 307
PshAI GACNNNNGTC 1 cut(s) 2781
Psp124BI GAGCTC 1 cut(s) 1029
Psp5II RGGWCCY 1 cut(s) 1217
Psp6I CCWGG 2 cut(s) 1587, 1759
PspFI CCCAGC 2 cut(s) 422, 2735
PspGI CCWGG 2 cut(s) 1587, 1759
PspN4I GGNNCC 9 cut(s) 120, 186, 768, 1174, 1219, 2164, 2391, 2643, 2741
PspPI GGNCC 8 cut(s) 85, 118, 161, 236, 257, 767, 1217, 2641
PspPPI RGGWCCY 1 cut(s) 1217
PstNI CAGNNNCTG 1 cut(s) 527
PsuI RGATCY 5 cut(s) 184, 1413, 1891, 2221, 2389
PvuI CGATCG 1 cut(s) 2598
PvuII CAGCTG 1 cut(s) 1858
RsaI GTAC 8 cut(s) 686, 724, 757, 1437, 1638, 2471, 2593, 2741
RsaNI GTAC 8 cut(s) 685, 723, 756, 1436, 1637, 2470, 2592, 2740
RseI CAYNNNNRTG 3 cut(s) 879, 1332, 2357
SacI GAGCTC 1 cut(s) 1029
SalI GTCGAC 1 cut(s) 292
SapI GCTCTTC 1 cut(s) 2824
SaqAI TTAA 6 cut(s) 399, 1157, 2006, 2169, 2177, 2565
SatI GCNGC 6 cut(s) 336, 528, 618, 746, 1101, 1856
Sau96I GGNCC 8 cut(s) 85, 118, 161, 236, 257, 767, 1217, 2641
ScrFI CCNGG 2 cut(s) 1589, 1761
SduI GDGCHC 4 cut(s) 379, 1029, 2265, 2442
SfaNI GCATC 6 cut(s) 277, 1045, 1211, 1570, 2301, 2722
SfcI CTRYAG 3 cut(s) 72, 1212, 2620
SfuI TTCGAA 1 cut(s) 1250
SinI GGWCC 6 cut(s) 161, 236, 257, 767, 1217, 2641
SmiMI CAYNNNNRTG 3 cut(s) 879, 1332, 2357
SmlI CTYRAG 1 cut(s) 2258
SmoI CTYRAG 1 cut(s) 2258
SpeI ACTAGT 1 cut(s) 1430
SspI AATATT 2 cut(s) 609, 1918
SstI GAGCTC 1 cut(s) 1029
StyD4I CCNGG 2 cut(s) 1587, 1759
StyI CCWWGG 1 cut(s) 220
TaaI ACNGT 6 cut(s) 171, 889, 996, 2036, 2238, 2267
TaiI ACGT 6 cut(s) 1038, 1110, 1448, 1555, 1803, 1811
TaqII GACCGA 3 cut(s) 178, 1255, 1529
TatI WGTACW 3 cut(s) 722, 1435, 2469
TauI GCSGC 1 cut(s) 748
TfiI GAWTC 6 cut(s) 216, 803, 1601, 1764, 2284, 2636
Tru1I TTAA 6 cut(s) 399, 1157, 2006, 2169, 2177, 2565
Tru9I TTAA 6 cut(s) 399, 1157, 2006, 2169, 2177, 2565
TscAI CASTG 5 cut(s) 471, 649, 1389, 1497, 2335
TseFI GTSAC 8 cut(s) 46, 303, 462, 676, 872, 1078, 1667, 2042
TseI GCWGC 5 cut(s) 335, 527, 617, 1100, 1855
Tsp45I GTSAC 8 cut(s) 46, 303, 462, 676, 872, 1078, 1667, 2042
TspDTI ATGAA 9 cut(s) 491, 816, 1557, 1928, 2086, 2165, 2231, 2276, 2430
TspGWI ACGGA 9 cut(s) 192, 356, 571, 1311, 1525, 1916, 1978, 2035, 2357
TspRI CASTG 5 cut(s) 471, 649, 1389, 1497, 2335
VpaK11BI GGWCC 6 cut(s) 161, 236, 257, 767, 1217, 2641
XapI RAATTY 1 cut(s) 1929
XbaI TCTAGA 1 cut(s) 1894
XceI RCATGY 4 cut(s) 311, 463, 617, 1855
XmiI GTMKAC 4 cut(s) 293, 370, 634, 1237
XmnI GAANNNNTTC 2 cut(s) 443, 2830
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.