aspartic-type endopeptidase activity
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 282 |
| Acc16I | TGCGCA | 1 cut(s) | 167 |
| Acc36I | ACCTGC | 1 cut(s) | 259 |
| AccII | CGCG | 1 cut(s) | 97 |
| AciI | CCGC | 4 cut(s) | 95, 97, 144, 326 |
| AclWI | GGATC | 1 cut(s) | 128 |
| AcsI | RAATTY | 1 cut(s) | 171 |
| AcyI | GRCGYC | 1 cut(s) | 279 |
| AdeI | CACNNNGTG | 1 cut(s) | 152 |
| AfaI | GTAC | 1 cut(s) | 316 |
| AluBI | AGCT | 3 cut(s) | 13, 53, 227 |
| AluI | AGCT | 3 cut(s) | 13, 53, 227 |
| Alw21I | GWGCWC | 1 cut(s) | 8 |
| Alw26I | GTCTC | 1 cut(s) | 129 |
| AlwI | GGATC | 1 cut(s) | 128 |
| AoxI | GGCC | 1 cut(s) | 71 |
| ApeKI | GCWGC | 2 cut(s) | 50, 254 |
| ApoI | RAATTY | 1 cut(s) | 171 |
| AspLEI | GCGC | 1 cut(s) | 168 |
| AspS9I | GGNCC | 2 cut(s) | 61, 72 |
| AvaII | GGWCC | 1 cut(s) | 61 |
| Bbv12I | GWGCWC | 1 cut(s) | 8 |
| BbvI | GCAGC | 2 cut(s) | 62, 241 |
| BccI | CCATC | 1 cut(s) | 299 |
| BcoDI | GTCTC | 1 cut(s) | 129 |
| BfaI | CTAG | 2 cut(s) | 17, 224 |
| BfmI | CTRYAG | 1 cut(s) | 182 |
| BfuAI | ACCTGC | 1 cut(s) | 259 |
| BisI | GCNGC | 3 cut(s) | 51, 255, 327 |
| BlsI | GCNGC | 3 cut(s) | 52, 256, 328 |
| Bme18I | GGWCC | 1 cut(s) | 61 |
| BmgT120I | GGNCC | 2 cut(s) | 61, 72 |
| BmiI | GGNNCC | 1 cut(s) | 63 |
| BsaAI | YACGTR | 1 cut(s) | 105 |
| BsaHI | GRCGYC | 1 cut(s) | 279 |
| BsaJI | CCNNGG | 2 cut(s) | 95, 321 |
| Bse1I | ACTGG | 1 cut(s) | 334 |
| BseDI | CCNNGG | 2 cut(s) | 95, 321 |
| BseGI | GGATG | 1 cut(s) | 296 |
| BseNI | ACTGG | 1 cut(s) | 334 |
| BseXI | GCAGC | 2 cut(s) | 62, 241 |
| Bsh1236I | CGCG | 1 cut(s) | 97 |
| BshFI | GGCC | 1 cut(s) | 73 |
| BsiHKAI | GWGCWC | 1 cut(s) | 8 |
| BslFI | GGGAC | 4 cut(s) | 47, 98, 284, 329 |
| BsmAI | GTCTC | 1 cut(s) | 129 |
| BsmFI | GGGAC | 4 cut(s) | 47, 98, 284, 329 |
| BsnI | GGCC | 1 cut(s) | 73 |
| Bsp1286I | GDGCHC | 1 cut(s) | 8 |
| Bsp143I | GATC | 3 cut(s) | 133, 220, 239 |
| Bsp19I | CCATGG | 1 cut(s) | 321 |
| BspACI | CCGC | 4 cut(s) | 95, 97, 144, 326 |
| BspANI | GGCC | 1 cut(s) | 73 |
| BspFNI | CGCG | 1 cut(s) | 97 |
| BspLI | GGNNCC | 1 cut(s) | 63 |
| BspMI | ACCTGC | 1 cut(s) | 259 |
| BspPI | GGATC | 1 cut(s) | 128 |
| BsrI | ACTGG | 1 cut(s) | 334 |
| BssECI | CCNNGG | 2 cut(s) | 95, 321 |
| BssMI | GATC | 3 cut(s) | 133, 220, 239 |
| BssNI | GRCGYC | 1 cut(s) | 279 |
| BssT1I | CCWWGG | 1 cut(s) | 321 |
| BstACI | GRCGYC | 1 cut(s) | 279 |
| BstBAI | YACGTR | 1 cut(s) | 105 |
| BstC8I | GCNNGC | 2 cut(s) | 48, 267 |
| BstDSI | CCRYGG | 2 cut(s) | 95, 321 |
| BstF5I | GGATG | 1 cut(s) | 296 |
| BstFNI | CGCG | 1 cut(s) | 97 |
| BstHHI | GCGC | 1 cut(s) | 168 |
| BstKTI | GATC | 3 cut(s) | 136, 223, 242 |
| BstMAI | GTCTC | 1 cut(s) | 129 |
| BstMBI | GATC | 3 cut(s) | 133, 220, 239 |
| BstNSI | RCATGY | 1 cut(s) | 50 |
| BstSFI | CTRYAG | 1 cut(s) | 182 |
| BstUI | CGCG | 1 cut(s) | 97 |
| BstV1I | GCAGC | 2 cut(s) | 62, 241 |
| BsuRI | GGCC | 1 cut(s) | 73 |
| BtgI | CCRYGG | 2 cut(s) | 95, 321 |
| BtsCI | GGATG | 1 cut(s) | 296 |
| BtsI | GCAGTG | 1 cut(s) | 75 |
| BtsIMutI | CAGTG | 1 cut(s) | 75 |
| BveI | ACCTGC | 1 cut(s) | 259 |
| Cac8I | GCNNGC | 2 cut(s) | 48, 267 |
| CfoI | GCGC | 1 cut(s) | 168 |
| Cfr13I | GGNCC | 2 cut(s) | 61, 72 |
| Cfr42I | CCGCGG | 1 cut(s) | 98 |
| Csp6I | GTAC | 1 cut(s) | 315 |
| CviAII | CATG | 3 cut(s) | 47, 284, 322 |
| CviJI | RGCY | 7 cut(s) | 13, 33, 53, 73, 194, 227, 329 |
| CviKI_1 | RGCY | 7 cut(s) | 13, 33, 53, 73, 194, 227, 329 |
| CviQI | GTAC | 1 cut(s) | 315 |
| DpnI | GATC | 3 cut(s) | 135, 222, 241 |
| DpnII | GATC | 3 cut(s) | 133, 220, 239 |
| DraIII | CACNNNGTG | 1 cut(s) | 152 |
| Eco130I | CCWWGG | 1 cut(s) | 321 |
| Eco47I | GGWCC | 1 cut(s) | 61 |
| EcoRI | GAATTC | 1 cut(s) | 171 |
| EcoT14I | CCWWGG | 1 cut(s) | 321 |
| ErhI | CCWWGG | 1 cut(s) | 321 |
| FaeI | CATG | 3 cut(s) | 50, 287, 325 |
| FaqI | GGGAC | 4 cut(s) | 47, 98, 284, 329 |
| FatI | CATG | 3 cut(s) | 46, 283, 321 |
| FauNDI | CATATG | 1 cut(s) | 340 |
| Fnu4HI | GCNGC | 3 cut(s) | 51, 255, 327 |
| FokI | GGATG | 1 cut(s) | 303 |
| Fsp4HI | GCNGC | 3 cut(s) | 51, 255, 327 |
| FspBI | CTAG | 2 cut(s) | 17, 224 |
| FspI | TGCGCA | 1 cut(s) | 167 |
| GlaI | GCGC | 1 cut(s) | 167 |
| GluI | GCNGC | 3 cut(s) | 51, 255, 327 |
| HaeIII | GGCC | 1 cut(s) | 73 |
| HhaI | GCGC | 1 cut(s) | 168 |
| Hin1I | GRCGYC | 1 cut(s) | 279 |
| Hin1II | CATG | 3 cut(s) | 50, 287, 325 |
| Hin6I | GCGC | 1 cut(s) | 166 |
| HinP1I | GCGC | 1 cut(s) | 166 |
| Hpy188I | TCNGA | 1 cut(s) | 220 |
| Hpy188III | TCNNGA | 1 cut(s) | 131 |
| HpyCH4IV | ACGT | 3 cut(s) | 104, 210, 279 |
| HpyCH4V | TGCA | 5 cut(s) | 38, 46, 50, 257, 269 |
| HpySE526I | ACGT | 3 cut(s) | 104, 210, 279 |
| Hsp92I | GRCGYC | 1 cut(s) | 279 |
| Hsp92II | CATG | 3 cut(s) | 50, 287, 325 |
| HspAI | GCGC | 1 cut(s) | 166 |
| KspI | CCGCGG | 1 cut(s) | 98 |
| Kzo9I | GATC | 3 cut(s) | 133, 220, 239 |
| LmnI | GCTCC | 1 cut(s) | 18 |
| LpnPI | CCDG | 5 cut(s) | 219, 264, 279, 315, 347 |
| Lsp1109I | GCAGC | 2 cut(s) | 62, 241 |
| MaeI | CTAG | 2 cut(s) | 17, 224 |
| MaeII | ACGT | 3 cut(s) | 104, 210, 279 |
| MaeIII | GTNAC | 1 cut(s) | 232 |
| MalI | GATC | 3 cut(s) | 135, 222, 241 |
| MboI | GATC | 3 cut(s) | 133, 220, 239 |
| MhlI | GDGCHC | 1 cut(s) | 8 |
| MluCI | AATT | 1 cut(s) | 171 |
| MnlI | CCTC | 2 cut(s) | 63, 305 |
| MspA1I | CMGCKG | 1 cut(s) | 97 |
| MvnI | CGCG | 1 cut(s) | 97 |
| NcoI | CCATGG | 1 cut(s) | 321 |
| NdeI | CATATG | 1 cut(s) | 340 |
| NdeII | GATC | 3 cut(s) | 133, 220, 239 |
| NlaIII | CATG | 3 cut(s) | 50, 287, 325 |
| NlaIV | GGNNCC | 1 cut(s) | 63 |
| NmuCI | GTSAC | 1 cut(s) | 232 |
| NsbI | TGCGCA | 1 cut(s) | 167 |
| NspI | RCATGY | 1 cut(s) | 50 |
| PaeI | GCATGC | 1 cut(s) | 50 |
| PkrI | GCNGC | 3 cut(s) | 52, 256, 328 |
| Ppu21I | YACGTR | 1 cut(s) | 105 |
| PspN4I | GGNNCC | 1 cut(s) | 63 |
| PspPI | GGNCC | 2 cut(s) | 61, 72 |
| RsaI | GTAC | 1 cut(s) | 316 |
| RsaNI | GTAC | 1 cut(s) | 315 |
| SacII | CCGCGG | 1 cut(s) | 98 |
| SatI | GCNGC | 3 cut(s) | 51, 255, 327 |
| Sau3AI | GATC | 3 cut(s) | 133, 220, 239 |
| Sau96I | GGNCC | 2 cut(s) | 61, 72 |
| SduI | GDGCHC | 1 cut(s) | 8 |
| SetI | ASST | 9 cut(s) | 15, 55, 90, 107, 213, 229, 253, 282, 316 |
| SfcI | CTRYAG | 1 cut(s) | 182 |
| Sfr303I | CCGCGG | 1 cut(s) | 98 |
| SgrBI | CCGCGG | 1 cut(s) | 98 |
| SinI | GGWCC | 1 cut(s) | 61 |
| SphI | GCATGC | 1 cut(s) | 50 |
| Sse9I | AATT | 1 cut(s) | 171 |
| SsiI | CCGC | 4 cut(s) | 95, 97, 144, 326 |
| SspI | AATATT | 1 cut(s) | 42 |
| SspMI | CTAG | 2 cut(s) | 17, 224 |
| StyI | CCWWGG | 1 cut(s) | 321 |
| TaiI | ACGT | 3 cut(s) | 107, 213, 282 |
| TasI | AATT | 1 cut(s) | 171 |
| TauI | GCSGC | 1 cut(s) | 329 |
| TscAI | CASTG | 1 cut(s) | 82 |
| TseFI | GTSAC | 1 cut(s) | 232 |
| TseI | GCWGC | 2 cut(s) | 50, 254 |
| Tsp45I | GTSAC | 1 cut(s) | 232 |
| TspDTI | ATGAA | 1 cut(s) | 174 |
| TspRI | CASTG | 1 cut(s) | 82 |
| VpaK11BI | GGWCC | 1 cut(s) | 61 |
| XapI | RAATTY | 1 cut(s) | 171 |
| XceI | RCATGY | 1 cut(s) | 50 |
| XspI | CTAG | 2 cut(s) | 17, 224 |
| ZraI | GACGTC | 1 cut(s) | 280 |