aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 215 |
| AcsI | RAATTY | 1 cut(s) | 422 |
| AdeI | CACNNNGTG | 1 cut(s) | 116 |
| AfaI | GTAC | 1 cut(s) | 415 |
| AfeI | AGCGCT | 1 cut(s) | 100 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 89 |
| AgsI | TTSAA | 2 cut(s) | 317, 422 |
| AjnI | CCWGG | 1 cut(s) | 507 |
| AluBI | AGCT | 1 cut(s) | 53 |
| AluI | AGCT | 1 cut(s) | 53 |
| Alw21I | GWGCWC | 1 cut(s) | 435 |
| Alw26I | GTCTC | 1 cut(s) | 268 |
| AlwI | GGATC | 1 cut(s) | 215 |
| Aor51HI | AGCGCT | 1 cut(s) | 100 |
| AoxI | GGCC | 2 cut(s) | 161, 485 |
| ApeKI | GCWGC | 2 cut(s) | 53, 101 |
| ApoI | RAATTY | 1 cut(s) | 422 |
| AseI | ATTAAT | 1 cut(s) | 227 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 321 |
| AspLEI | GCGC | 1 cut(s) | 101 |
| AspS9I | GGNCC | 1 cut(s) | 486 |
| AsuHPI | GGTGA | 1 cut(s) | 176 |
| BaeGI | GKGCMC | 1 cut(s) | 96 |
| Bbv12I | GWGCWC | 1 cut(s) | 435 |
| BbvI | GCAGC | 2 cut(s) | 40, 88 |
| BciT130I | CCWGG | 1 cut(s) | 509 |
| BcoDI | GTCTC | 1 cut(s) | 268 |
| BfaI | CTAG | 2 cut(s) | 326, 518 |
| BfoI | RGCGCY | 1 cut(s) | 102 |
| BglII | AGATCT | 1 cut(s) | 380 |
| BisI | GCNGC | 2 cut(s) | 54, 102 |
| BlsI | GCNGC | 2 cut(s) | 55, 103 |
| Bme1390I | CCNGG | 1 cut(s) | 509 |
| BmgT120I | GGNCC | 1 cut(s) | 486 |
| BmrFI | CCNGG | 1 cut(s) | 509 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 233, 265, 248, 280 |
| BpmI | CTGGAG | 1 cut(s) | 317 |
| BsaJI | CCNNGG | 1 cut(s) | 188 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 111, 141 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 89 |
| Bse1I | ACTGG | 1 cut(s) | 300 |
| BseBI | CCWGG | 1 cut(s) | 509 |
| BseDI | CCNNGG | 1 cut(s) | 188 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 89 |
| BseNI | ACTGG | 1 cut(s) | 300 |
| BseSI | GKGCMC | 1 cut(s) | 96 |
| BseXI | GCAGC | 2 cut(s) | 40, 88 |
| BshFI | GGCC | 2 cut(s) | 163, 487 |
| BsiHKAI | GWGCWC | 1 cut(s) | 435 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 89 |
| BsmAI | GTCTC | 1 cut(s) | 268 |
| BsnI | GGCC | 2 cut(s) | 163, 487 |
| Bsp1286I | GDGCHC | 2 cut(s) | 96, 435 |
| Bsp143I | GATC | 3 cut(s) | 38, 220, 380 |
| BspANI | GGCC | 2 cut(s) | 163, 487 |
| BspHI | TCATGA | 1 cut(s) | 253 |
| BspPI | GGATC | 1 cut(s) | 215 |
| BsrI | ACTGG | 1 cut(s) | 300 |
| BssECI | CCNNGG | 1 cut(s) | 188 |
| BssMI | GATC | 3 cut(s) | 38, 220, 380 |
| BssT1I | CCWWGG | 1 cut(s) | 188 |
| Bst2UI | CCWGG | 1 cut(s) | 509 |
| Bst4CI | ACNGT | 2 cut(s) | 359, 418 |
| Bst6I | CTCTTC | 2 cut(s) | 146, 191 |
| BstC8I | GCNNGC | 1 cut(s) | 66 |
| BstDEI | CTNAG | 1 cut(s) | 113 |
| BstH2I | RGCGCY | 1 cut(s) | 102 |
| BstHHI | GCGC | 1 cut(s) | 101 |
| BstKTI | GATC | 3 cut(s) | 41, 223, 383 |
| BstMAI | GTCTC | 1 cut(s) | 268 |
| BstMBI | GATC | 3 cut(s) | 38, 220, 380 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 209 |
| BstNI | CCWGG | 1 cut(s) | 509 |
| BstNSI | RCATGY | 1 cut(s) | 414 |
| BstSCI | CCNGG | 1 cut(s) | 507 |
| BstSLI | GKGCMC | 1 cut(s) | 96 |
| BstV1I | GCAGC | 2 cut(s) | 40, 88 |
| BstX2I | RGATCY | 1 cut(s) | 380 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 171 |
| BstYI | RGATCY | 1 cut(s) | 380 |
| BsuRI | GGCC | 2 cut(s) | 163, 487 |
| BtsIMutI | CAGTG | 1 cut(s) | 364 |
| Cac8I | GCNNGC | 1 cut(s) | 66 |
| CciI | TCATGA | 1 cut(s) | 253 |
| CfoI | GCGC | 1 cut(s) | 101 |
| Cfr13I | GGNCC | 1 cut(s) | 486 |
| CseI | GACGC | 1 cut(s) | 311 |
| Csp6I | GTAC | 1 cut(s) | 414 |
| CviAII | CATG | 5 cut(s) | 167, 254, 284, 411, 450 |
| CviJI | RGCY | 5 cut(s) | 53, 134, 140, 163, 487 |
| CviKI_1 | RGCY | 5 cut(s) | 53, 134, 140, 163, 487 |
| CviQI | GTAC | 1 cut(s) | 414 |
| DdeI | CTNAG | 1 cut(s) | 113 |
| DpnI | GATC | 3 cut(s) | 40, 222, 382 |
| DpnII | GATC | 3 cut(s) | 38, 220, 380 |
| DraIII | CACNNNGTG | 1 cut(s) | 116 |
| Eam1104I | CTCTTC | 2 cut(s) | 146, 191 |
| EarI | CTCTTC | 2 cut(s) | 146, 191 |
| Eco130I | CCWWGG | 1 cut(s) | 188 |
| Eco32I | GATATC | 2 cut(s) | 22, 442 |
| Eco47III | AGCGCT | 1 cut(s) | 100 |
| EcoRI | GAATTC | 1 cut(s) | 422 |
| EcoRII | CCWGG | 1 cut(s) | 507 |
| EcoRV | GATATC | 2 cut(s) | 22, 442 |
| EcoT14I | CCWWGG | 1 cut(s) | 188 |
| ErhI | CCWWGG | 1 cut(s) | 188 |
| FaeI | CATG | 5 cut(s) | 170, 257, 287, 414, 453 |
| FatI | CATG | 5 cut(s) | 166, 253, 283, 410, 449 |
| Fnu4HI | GCNGC | 2 cut(s) | 54, 102 |
| Fsp4HI | GCNGC | 2 cut(s) | 54, 102 |
| FspBI | CTAG | 2 cut(s) | 326, 518 |
| GlaI | GCGC | 1 cut(s) | 100 |
| GluI | GCNGC | 2 cut(s) | 54, 102 |
| GsuI | CTGGAG | 1 cut(s) | 317 |
| HaeII | RGCGCY | 1 cut(s) | 102 |
| HaeIII | GGCC | 2 cut(s) | 163, 487 |
| HgaI | GACGC | 1 cut(s) | 311 |
| HhaI | GCGC | 1 cut(s) | 101 |
| Hin1II | CATG | 5 cut(s) | 170, 257, 287, 414, 453 |
| Hin6I | GCGC | 1 cut(s) | 99 |
| HinP1I | GCGC | 1 cut(s) | 99 |
| HincII | GTYRAC | 1 cut(s) | 470 |
| HindII | GTYRAC | 1 cut(s) | 470 |
| HinfI | GANTC | 5 cut(s) | 175, 244, 287, 322, 521 |
| HphI | GGTGA | 1 cut(s) | 176 |
| Hpy166II | GTNNAC | 1 cut(s) | 470 |
| Hpy188I | TCNGA | 2 cut(s) | 268, 309 |
| Hpy188III | TCNNGA | 2 cut(s) | 254, 326 |
| Hpy8I | GTNNAC | 1 cut(s) | 470 |
| HpyCH4III | ACNGT | 2 cut(s) | 359, 418 |
| HpyCH4V | TGCA | 5 cut(s) | 56, 68, 104, 410, 461 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 209 |
| HpyF3I | CTNAG | 1 cut(s) | 113 |
| Hsp92II | CATG | 5 cut(s) | 170, 257, 287, 414, 453 |
| HspAI | GCGC | 1 cut(s) | 99 |
| Kzo9I | GATC | 3 cut(s) | 38, 220, 380 |
| LmnI | GCTCC | 1 cut(s) | 310 |
| LpnPI | CCDG | 7 cut(s) | 18, 63, 78, 281, 494, 512, 521 |
| Lsp1109I | GCAGC | 2 cut(s) | 40, 88 |
| MaeI | CTAG | 2 cut(s) | 326, 518 |
| MaeIII | GTNAC | 1 cut(s) | 31 |
| MalI | GATC | 3 cut(s) | 40, 222, 382 |
| MboI | GATC | 3 cut(s) | 38, 220, 380 |
| MboII | GAAGA | 4 cut(s) | 163, 205, 208, 375 |
| MflI | RGATCY | 1 cut(s) | 380 |
| MhlI | GDGCHC | 2 cut(s) | 96, 435 |
| MluCI | AATT | 1 cut(s) | 422 |
| MmeI | TCCRAC | 1 cut(s) | 152 |
| MnlI | CCTC | 7 cut(s) | 113, 124, 147, 153, 192, 259, 292 |
| MroXI | GAANNNNTTC | 1 cut(s) | 321 |
| MseI | TTAA | 1 cut(s) | 227 |
| MslI | CAYNNNNRTG | 1 cut(s) | 396 |
| MspA1I | CMGCKG | 1 cut(s) | 53 |
| MspR9I | CCNGG | 1 cut(s) | 509 |
| MvaI | CCWGG | 1 cut(s) | 509 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 209 |
| NdeII | GATC | 3 cut(s) | 38, 220, 380 |
| NlaIII | CATG | 5 cut(s) | 170, 257, 287, 414, 453 |
| NmuCI | GTSAC | 1 cut(s) | 31 |
| NspI | RCATGY | 1 cut(s) | 414 |
| PagI | TCATGA | 1 cut(s) | 253 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 441 |
| PdmI | GAANNNNTTC | 1 cut(s) | 321 |
| PfeI | GAWTC | 5 cut(s) | 175, 244, 287, 322, 521 |
| PkrI | GCNGC | 2 cut(s) | 55, 103 |
| PshBI | ATTAAT | 1 cut(s) | 227 |
| Psp6I | CCWGG | 1 cut(s) | 507 |
| PspGI | CCWGG | 1 cut(s) | 507 |
| PspPI | GGNCC | 1 cut(s) | 486 |
| PsuI | RGATCY | 1 cut(s) | 380 |
| PvuII | CAGCTG | 1 cut(s) | 53 |
| RsaI | GTAC | 1 cut(s) | 415 |
| RsaNI | GTAC | 1 cut(s) | 414 |
| RseI | CAYNNNNRTG | 1 cut(s) | 396 |
| SaqAI | TTAA | 1 cut(s) | 227 |
| SatI | GCNGC | 2 cut(s) | 54, 102 |
| Sau3AI | GATC | 3 cut(s) | 38, 220, 380 |
| Sau96I | GGNCC | 1 cut(s) | 486 |
| ScrFI | CCNGG | 1 cut(s) | 509 |
| SduI | GDGCHC | 2 cut(s) | 96, 435 |
| SetI | ASST | 4 cut(s) | 55, 74, 124, 513 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 396 |
| Sse9I | AATT | 1 cut(s) | 422 |
| SspMI | CTAG | 2 cut(s) | 326, 518 |
| StyD4I | CCNGG | 1 cut(s) | 507 |
| StyI | CCWWGG | 1 cut(s) | 188 |
| TaaI | ACNGT | 2 cut(s) | 359, 418 |
| TaqI | TCGA | 2 cut(s) | 386, 444 |
| TasI | AATT | 1 cut(s) | 422 |
| TatI | WGTACW | 1 cut(s) | 413 |
| TfiI | GAWTC | 5 cut(s) | 175, 244, 287, 322, 521 |
| Tru1I | TTAA | 1 cut(s) | 227 |
| Tru9I | TTAA | 1 cut(s) | 227 |
| TscAI | CASTG | 1 cut(s) | 364 |
| TseFI | GTSAC | 1 cut(s) | 31 |
| TseI | GCWGC | 2 cut(s) | 53, 101 |
| Tsp45I | GTSAC | 1 cut(s) | 31 |
| TspDTI | ATGAA | 2 cut(s) | 300, 415 |
| TspRI | CASTG | 1 cut(s) | 364 |
| VspI | ATTAAT | 1 cut(s) | 227 |
| XapI | RAATTY | 1 cut(s) | 422 |
| XbaI | TCTAGA | 1 cut(s) | 325 |
| XceI | RCATGY | 1 cut(s) | 414 |
| XmnI | GAANNNNTTC | 1 cut(s) | 321 |
| XspI | CTAG | 2 cut(s) | 326, 518 |