aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccBSI | CCGCTC | 1 cut(s) | 351 |
| AciI | CCGC | 2 cut(s) | 82, 349 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 88 |
| AjnI | CCWGG | 1 cut(s) | 405 |
| AoxI | GGCC | 2 cut(s) | 147, 383 |
| AsuHPI | GGTGA | 1 cut(s) | 191 |
| BaeGI | GKGCMC | 1 cut(s) | 96 |
| BciT130I | CCWGG | 1 cut(s) | 407 |
| BfaI | CTAG | 1 cut(s) | 416 |
| BfmI | CTRYAG | 1 cut(s) | 102 |
| Bme1390I | CCNGG | 1 cut(s) | 407 |
| BmiI | GGNNCC | 1 cut(s) | 156 |
| BmrFI | CCNGG | 1 cut(s) | 407 |
| BpuEI | CTTGAG | 1 cut(s) | 300 |
| BsaJI | CCNNGG | 2 cut(s) | 150, 203 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 126, 156 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 88 |
| Bse1I | ACTGG | 1 cut(s) | 50 |
| BseBI | CCWGG | 1 cut(s) | 407 |
| BseDI | CCNNGG | 2 cut(s) | 150, 203 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 88 |
| BseNI | ACTGG | 1 cut(s) | 50 |
| BseSI | GKGCMC | 1 cut(s) | 96 |
| BshFI | GGCC | 2 cut(s) | 149, 385 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 88 |
| BsnI | GGCC | 2 cut(s) | 149, 385 |
| Bsp1286I | GDGCHC | 1 cut(s) | 96 |
| Bsp143I | GATC | 2 cut(s) | 38, 328 |
| BspACI | CCGC | 2 cut(s) | 82, 349 |
| BspANI | GGCC | 2 cut(s) | 149, 385 |
| BspLI | GGNNCC | 1 cut(s) | 156 |
| BsrBI | CCGCTC | 1 cut(s) | 351 |
| BsrI | ACTGG | 1 cut(s) | 50 |
| BssECI | CCNNGG | 2 cut(s) | 150, 203 |
| BssMI | GATC | 2 cut(s) | 38, 328 |
| BssT1I | CCWWGG | 2 cut(s) | 150, 203 |
| Bst2UI | CCWGG | 1 cut(s) | 407 |
| Bst4CI | ACNGT | 1 cut(s) | 310 |
| Bst6I | CTCTTC | 1 cut(s) | 161 |
| BstC8I | GCNNGC | 1 cut(s) | 66 |
| BstDEI | CTNAG | 1 cut(s) | 128 |
| BstKTI | GATC | 2 cut(s) | 41, 331 |
| BstMBI | GATC | 2 cut(s) | 38, 328 |
| BstNI | CCWGG | 1 cut(s) | 407 |
| BstSCI | CCNGG | 1 cut(s) | 405 |
| BstSFI | CTRYAG | 1 cut(s) | 102 |
| BstSLI | GKGCMC | 1 cut(s) | 96 |
| BsuRI | GGCC | 2 cut(s) | 149, 385 |
| BtsI | GCAGTG | 1 cut(s) | 114 |
| BtsIMutI | CAGTG | 2 cut(s) | 114, 306 |
| Cac8I | GCNNGC | 1 cut(s) | 66 |
| CviAII | CATG | 1 cut(s) | 390 |
| CviJI | RGCY | 4 cut(s) | 149, 155, 178, 385 |
| CviKI_1 | RGCY | 4 cut(s) | 149, 155, 178, 385 |
| DdeI | CTNAG | 1 cut(s) | 128 |
| DpnI | GATC | 2 cut(s) | 40, 330 |
| DpnII | GATC | 2 cut(s) | 38, 328 |
| Eam1104I | CTCTTC | 1 cut(s) | 161 |
| EarI | CTCTTC | 1 cut(s) | 161 |
| Eco130I | CCWWGG | 2 cut(s) | 150, 203 |
| Eco147I | AGGCCT | 1 cut(s) | 149 |
| Eco32I | GATATC | 2 cut(s) | 22, 340 |
| EcoRII | CCWGG | 1 cut(s) | 405 |
| EcoRV | GATATC | 2 cut(s) | 22, 340 |
| EcoT14I | CCWWGG | 2 cut(s) | 150, 203 |
| ErhI | CCWWGG | 2 cut(s) | 150, 203 |
| FaeI | CATG | 1 cut(s) | 393 |
| FaiI | YATR | 9 cut(s) | 45, 47, 125, 258, 281, 285, 304, 326, 391 |
| FatI | CATG | 1 cut(s) | 389 |
| FauI | CCCGC | 1 cut(s) | 356 |
| FspBI | CTAG | 1 cut(s) | 416 |
| HaeIII | GGCC | 2 cut(s) | 149, 385 |
| Hin1II | CATG | 1 cut(s) | 393 |
| HinfI | GANTC | 1 cut(s) | 419 |
| HphI | GGTGA | 1 cut(s) | 191 |
| Hpy166II | GTNNAC | 1 cut(s) | 15 |
| Hpy188III | TCNNGA | 2 cut(s) | 317, 332 |
| Hpy8I | GTNNAC | 1 cut(s) | 15 |
| HpyCH4III | ACNGT | 1 cut(s) | 310 |
| HpyCH4V | TGCA | 4 cut(s) | 68, 119, 302, 359 |
| HpyF3I | CTNAG | 1 cut(s) | 128 |
| Hsp92II | CATG | 1 cut(s) | 393 |
| Kzo9I | GATC | 2 cut(s) | 38, 328 |
| LmnI | GCTCC | 1 cut(s) | 160 |
| LpnPI | CCDG | 4 cut(s) | 63, 78, 392, 419 |
| MaeI | CTAG | 1 cut(s) | 416 |
| MaeIII | GTNAC | 1 cut(s) | 31 |
| MalI | GATC | 2 cut(s) | 40, 330 |
| MbiI | CCGCTC | 1 cut(s) | 351 |
| MboI | GATC | 2 cut(s) | 38, 328 |
| MboII | GAAGA | 3 cut(s) | 178, 220, 232 |
| MhlI | GDGCHC | 1 cut(s) | 96 |
| MnlI | CCTC | 4 cut(s) | 104, 128, 139, 162 |
| MslI | CAYNNNNRTG | 1 cut(s) | 288 |
| MspR9I | CCNGG | 1 cut(s) | 407 |
| MvaI | CCWGG | 1 cut(s) | 407 |
| NdeII | GATC | 2 cut(s) | 38, 328 |
| NlaIII | CATG | 1 cut(s) | 393 |
| NlaIV | GGNNCC | 1 cut(s) | 156 |
| NmuCI | GTSAC | 1 cut(s) | 31 |
| PceI | AGGCCT | 1 cut(s) | 149 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 339 |
| PfeI | GAWTC | 1 cut(s) | 419 |
| Psp6I | CCWGG | 1 cut(s) | 405 |
| PspGI | CCWGG | 1 cut(s) | 405 |
| PspN4I | GGNNCC | 1 cut(s) | 156 |
| RseI | CAYNNNNRTG | 1 cut(s) | 288 |
| Sau3AI | GATC | 2 cut(s) | 38, 328 |
| ScrFI | CCNGG | 1 cut(s) | 407 |
| SduI | GDGCHC | 1 cut(s) | 96 |
| SetI | ASST | 3 cut(s) | 74, 139, 411 |
| SfcI | CTRYAG | 1 cut(s) | 102 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 288 |
| SmlI | CTYRAG | 1 cut(s) | 315 |
| SmoI | CTYRAG | 1 cut(s) | 315 |
| SseBI | AGGCCT | 1 cut(s) | 149 |
| SsiI | CCGC | 2 cut(s) | 82, 349 |
| SspMI | CTAG | 1 cut(s) | 416 |
| StuI | AGGCCT | 1 cut(s) | 149 |
| StyD4I | CCNGG | 1 cut(s) | 405 |
| StyI | CCWWGG | 2 cut(s) | 150, 203 |
| TaaI | ACNGT | 1 cut(s) | 310 |
| TaqI | TCGA | 2 cut(s) | 188, 342 |
| TfiI | GAWTC | 1 cut(s) | 419 |
| TscAI | CASTG | 2 cut(s) | 121, 313 |
| TseFI | GTSAC | 1 cut(s) | 31 |
| Tsp45I | GTSAC | 1 cut(s) | 31 |
| TspRI | CASTG | 2 cut(s) | 121, 313 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 210 |
| XspI | CTAG | 1 cut(s) | 416 |