FvH4_6g44542

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000161
Physical Location & Seq
Reverse (-)
34305598 .. 34308138
2541 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g44542.t1

Sequence Viewer

Length: 2541 bp
ATGGGTCCTCATAGACGATTGGGTATCTACGTTGGTTATGAATCTCTAACGATTATCAGATATCTCGAGCCATTAACAGGTGATCTCTTTACCGCAAGGTTTGCGGATTGTCACTTTGATGAGACACTCTTCCCATCGTTAGGGGGAGATACGAACAAACGTGATCATGTGGAACGACAGGAATTGTCGTGGTTTGTCCCCACTCTGTCTCATTATGATCCCCACACCTCACAATCTGAACGTGAAGTGAAATGTATTCTCGAACTTCAGAAAGTAGCAGATACGATGCCTGACTCTTTCTCCGAGATTGCGAAAGTGACAAGATCAGATATACCAGCTGCAAATGTTCCTGCTAGGCTCGAAGTCCCTAGAAAGGGCACGGCCATCCCCGGGCTCGGTACTGCCGCCGTCGACGGCGCCGCCCATGGTGGAGGGATGGAGGCCGTGGCTCCCCAAAGGAAAAGGGGGAGGCCACCTGGTTCGGTTGACATGCGTCCGAGAAAGAAACTGACCGACAAGGCACAAGTCAATCCTTTGATCATCGATGTTGAGAATCCTTCTCACGAGATCATCTCTGATTACAGTTATGTCCACGAGTTGATATTAGAATCCATGGGGTACGCCTCAGGGTCAATGTCAACATCAGAGAACATAGAAATCTCCATGTGTTATGATAACACTTATGAATTGATGAACCGGTCATCAACCCATGTCGATGATATATTTGCTTATTCCGTGGCTCATGAAATCATTGAGCACGATGACATCGAACCACGCTCTGTTGCAGAATGCCAAAACAGAGCAGATTGGCCTAAATGGAAAGTTGCAATCCAGGCAGAATTAGACTCTCTAACAAAGAGACAGGTCTTCGGCCCAGTAATGCTAACTCCGCCGAGTGTAAAGCCTGTTGGACATAAATGGGTCTTTGTTAGAAAGCGTAATGAGAAGAATGAGGTACTTCTGTATAAGGCCCGCCTTGTGGCGCAAGGTTTCTCACAACGCCCTGGGATTGATTATGAGGAGACTTACTCTCCAGTCATGGACGTCATAACTTTTCGCTACTTAGTCAGTTTGGTAGTGTCCGAAAAACTTGACATGCAGCTCATGGATGTGGTTACGGCATATCTCTATGGGGATCTAGATTCAGAGATATATATGAAAGTGCCTGATGGCATTACGTTACCTAAATCAAGTAACTCTAAACCACGGAGTGCGTATGCTATAAGGTTGCAACGCTCACTTTACGGATTAAAGCAATCTGGGCGAATGTGGTATACTCGTCTAAGTGACTACTTGATTAGGGAGGGATATAAGAACGATGAACTATGCCCGTGCGTGTTCATAAAGAAGACAAGTTCCGGATTTGCAATAGTGGCCGTTTATGTCGATGATATGAACATTATCGGTACTCTTGATGAAATCAAAGAGACCGCGGCTTATCTGAAATCCGAGTTTGAGATGAAAGATCTTGGGAAGACTCGTTTTTGCCTAGGTCTTGAACTAGAGCACCGAGTCTGTGGGATCCTTATCTATCAGACTGCATATGTCCAAAAGATGCTCAGGCGATTTAATATGGACAAGATGCATCCTGTTAGCACTCCCATGATTGGTCGAAGTCTGGATATAACTAAAGATCCATTCCGTCCGAAAGAGGACGACGAAGAGGTCTTGAGCGCAGAGGTTCCATACCTTAGTGCAATAGGCGCGTTATTGTACTTAGCTCAATGCACTCGACCAGACATTGCATTCTCAGTCAACTTGTTAGCTAGATTTAGCTCAGCGCCAACCCAACGTCATTGGACCGGAGTTAAGAACATCTTTCGATATCTTAAAGGGACCATTGATTTGGGACTGTTCTTTTCCTACACTGATTCAAGAAGGACAGAAGATGGTCCTATGGCTCATGGAAGACACAGCACCGATCCACCAAGGGACCGGACTGCCGCCATGGACGCCGCCGGGACGGCTGCTGGACACGGGTCTAATGCCGTGCACGCCACCGCCGCCTTGTCCTATGCCGAGACACCCAACAATACGTTGGTGGGTTTCGCCGACGCCGGATATTTATCTGATCCTCATAAAGGACGTTCTCAAACTGGTTATGTTTTCACCATTGGGAATACGGCGATATCTTGGAGGTCTACTAAGCAGACTCTAGTGGCGACATCTTCAAATCATGCCGAGATTATAGCTTTGCATGAAGCTGTTCGGGAATGTGTTTGGTTGAGATCAATCATTAAGCACGTTAGAGGAACTAGTGGATTAAGTTCTACAACTGATAGACCTACATGCATTTATGAGGACAATGCAGCTTGTATTGAGCAAATGAAGTTAGGTTACATCAAGGGTGATAATACAAAGCACATTTCACCTAAGTTCTTCTACAACCAACAGCAACAGGCGTTTCTCAACATTCAAGTGAACCAAGTTAGTTCCGAAGGAAACGCAGCAGACTTATTTACTAAGTCCTTACCTAAGTCTACTTTTGAGAAACATGTGAAAAGTATAGGCATGCGTCGGCTATCAAGTCTCTCTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

847

Amino Acids

94.8

Weight (kDa)

6.7

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_2 PF07727 296 - 536 3.2e-60 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000161)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36192 FvH4_3g41702 FvH4_4g13933 FvH4_6g39331 FvH4_6g44542
malus_domestica MD17G1101700.v1.1
pyrus_communis pycom01g01810 pycom01g03340 pycom05g22610 pycom13g03220 pycom13g23770 pycom13g26870 pycom17g17400 pycom17g17410 pycom17g17890
rosa_chinensis RchiOBHm_Chr2g0129881 RchiOBHm_Chr2g0163801 RchiOBHm_Chr4g0392871 RchiOBHm_Chr7g0218471
rosa_multiflora Rmu_co8005914.1_g000001 Rmu_co8214666.1_g000002 Rmu_co8253889.1_g000001 Rmu_co8429419.1_g000001 Rmu_sc0000031.1_g000049 Rmu_sc0000091.1_g000004 Rmu_sc0000103.1_g000050 Rmu_sc0000147.1_g000047 Rmu_sc0000185.1_g000026 Rmu_sc0000235.1_g000056 Rmu_sc0000247.1_g000002 Rmu_sc0000310.1_g000010 Rmu_sc0000353.1_g000034 Rmu_sc0000353.1_g000035 Rmu_sc0000367.1_g000046 Rmu_sc0000420.1_g000034 Rmu_sc0000461.1_g000022 Rmu_sc0000544.1_g000007 Rmu_sc0000544.1_g000009 Rmu_sc0000607.1_g000032 Rmu_sc0000647.1_g000011 Rmu_sc0000675.1_g000049 Rmu_sc0000680.1_g000015 Rmu_sc0000795.1_g000112 Rmu_sc0000851.1_g000013 Rmu_sc0000868.1_g000019 Rmu_sc0000869.1_g000009 Rmu_sc0000898.1_g000061 Rmu_sc0000990.1_g000006 Rmu_sc0001009.1_g000006 Rmu_sc0001051.1_g000034 Rmu_sc0001067.1_g000005 Rmu_sc0001124.1_g000020 Rmu_sc0001142.1_g000016 Rmu_sc0001231.1_g000015 Rmu_sc0001235.1_g000013 Rmu_sc0001336.1_g000036 Rmu_sc0001552.1_g000033 Rmu_sc0001604.1_g000003 Rmu_sc0001605.1_g000009 Rmu_sc0001605.1_g000011 Rmu_sc0001668.1_g000025 Rmu_sc0001698.1_g000009 Rmu_sc0001721.1_g000010 Rmu_sc0001735.1_g000004 Rmu_sc0001832.1_g000017 Rmu_sc0001844.1_g000024 Rmu_sc0001880.1_g000026 Rmu_sc0001896.1_g000018 Rmu_sc0001918.1_g000011 Rmu_sc0002072.1_g000022 Rmu_sc0002109.1_g000003 Rmu_sc0002142.1_g000017 Rmu_sc0002166.1_g000001 Rmu_sc0002207.1_g000015 Rmu_sc0002346.1_g000001 Rmu_sc0002460.1_g000009 Rmu_sc0002460.1_g000043 Rmu_sc0002460.1_g000071 Rmu_sc0002672.1_g000001 Rmu_sc0002699.1_g000019 Rmu_sc0002736.1_g000007 Rmu_sc0002773.1_g000003 Rmu_sc0002893.1_g000006 Rmu_sc0002968.1_g000014 Rmu_sc0002968.1_g000015 Rmu_sc0003032.1_g000020 Rmu_sc0003044.1_g000024 Rmu_sc0003200.1_g000007 Rmu_sc0003261.1_g000031 Rmu_sc0003347.1_g000014 Rmu_sc0003385.1_g000019 Rmu_sc0003385.1_g000020 Rmu_sc0003419.1_g000017 Rmu_sc0003605.1_g000029 Rmu_sc0003679.1_g000010 Rmu_sc0003708.1_g000002 Rmu_sc0003737.1_g000033 Rmu_sc0003981.1_g000021 Rmu_sc0004101.1_g000009 Rmu_sc0004119.1_g000009 Rmu_sc0004248.1_g000021 Rmu_sc0004362.1_g000013 Rmu_sc0004520.1_g000020 Rmu_sc0004741.1_g000017 Rmu_sc0004771.1_g000007 Rmu_sc0004835.1_g000027 Rmu_sc0004969.1_g000010 Rmu_sc0005037.1_g000002 Rmu_sc0005272.1_g000006 Rmu_sc0005584.1_g000017 Rmu_sc0005652.1_g000005 Rmu_sc0005670.1_g000010 Rmu_sc0005781.1_g000035 Rmu_sc0005816.1_g000026 Rmu_sc0005852.1_g000003 Rmu_sc0006087.1_g000008 Rmu_sc0006152.1_g000005 Rmu_sc0006160.1_g000001 Rmu_sc0006313.1_g000014 Rmu_sc0006347.1_g000001 Rmu_sc0006583.1_g000024 Rmu_sc0006711.1_g000018 Rmu_sc0006952.1_g000003 Rmu_sc0006976.1_g000006 Rmu_sc0007115.1_g000006 Rmu_sc0007490.1_g000028 Rmu_sc0007740.1_g000003 Rmu_sc0007779.1_g000001 Rmu_sc0007907.1_g000003 Rmu_sc0007967.1_g000003 Rmu_sc0008456.1_g000006 Rmu_sc0008578.1_g000003 Rmu_sc0008642.1_g000001 Rmu_sc0008848.1_g000001 Rmu_sc0010065.1_g000009 Rmu_sc0010263.1_g000003 Rmu_sc0010632.1_g000004 Rmu_sc0011050.1_g000004 Rmu_sc0011563.1_g000005 Rmu_sc0011854.1_g000001 Rmu_sc0012216.1_g000003 Rmu_sc0012689.1_g000002 Rmu_sc0012945.1_g000001 Rmu_sc0013071.1_g000003 Rmu_sc0013305.1_g000008 Rmu_sc0013698.1_g000009 Rmu_sc0013772.1_g000007 Rmu_sc0013874.1_g000001 Rmu_sc0014217.1_g000001 Rmu_sc0014478.1_g000019 Rmu_sc0014724.1_g000002 Rmu_sc0016563.1_g000002 Rmu_sc0016563.1_g000003 Rmu_sc0017108.1_g000001 Rmu_sc0017408.1_g000002 Rmu_sc0020248.1_g000001 Rmu_sc0021416.1_g000005 Rmu_sc0022221.1_g000003 Rmu_sc0022402.1_g000002 Rmu_sc0023172.1_g000001 Rmu_sc0024783.1_g000001 Rmu_sc0028152.1_g000001 Rmu_sc0028541.1_g000001 Rmu_sc0029935.1_g000001 Rmu_sc0030041.1_g000001 Rmu_sc0035924.1_g000001 Rmu_sc0038113.1_g000002 Rmu_sc0040377.1_g000001 Rmu_ssc0000041.1_g000024 Rmu_ssc0000197.1_g000066 Rmu_ssc0000213.1_g000082 Rmu_ssc0000215.1_g000018
rosa_roxburghii Rroxscaffold_152G00434500 Rroxscaffold_3G00223490 Rroxscaffold_3G00226440 Rroxscaffold_3G00237960 Rroxscaffold_6G00394150
rosa_samantha Rh5CG379600 Rh5CG449200 Rh6DG448500
rosa_wichuraiana Rw0G004320 Rw1G010800 Rw2G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1664
AatII GACGTC 1 cut(s) 1047
AccB1I GGYRCC 1 cut(s) 416
AccI GTMKAC 4 cut(s) 411, 1274, 2141, 2480
AccII CGCG 2 cut(s) 1433, 1706
AccIII TCCGGA 1 cut(s) 1358
AclWI GGATC 7 cut(s) 212, 1143, 1516, 1529, 1628, 1916, 2066
AcoI YGGCCR 2 cut(s) 381, 1374
AcuI CTGAAG 1 cut(s) 251
AcyI GRCGYC 4 cut(s) 417, 1044, 1953, 2055
AdeI CACNNNGTG 1 cut(s) 1211
AfaI GTAC 5 cut(s) 400, 620, 957, 1408, 1715
AfiI CCNNNNNNNGG 9 cut(s) 77, 140, 373, 374, 390, 395, 979, 1607, 2081
AflIII ACRYGT 1 cut(s) 2494
AgeI ACCGGT 1 cut(s) 696
AgsI TTSAA 4 cut(s) 1499, 1875, 2172, 2417
AhlI ACTAGT 1 cut(s) 2255
AjnI CCWGG 3 cut(s) 475, 831, 1003
AluBI AGCT 8 cut(s) 338, 1102, 1721, 1766, 1776, 2192, 2204, 2312
AluI AGCT 8 cut(s) 338, 1102, 1721, 1766, 1776, 2192, 2204, 2312
Alw21I GWGCWC 3 cut(s) 759, 1509, 1995
Alw26I GTCTC 7 cut(s) 116, 213, 853, 1016, 1421, 2015, 2534
Alw44I GTGCAC 1 cut(s) 1991
AlwI GGATC 7 cut(s) 212, 1143, 1516, 1529, 1628, 1916, 2066
Ama87I CYCGRG 2 cut(s) 65, 389
Aor13HI TCCGGA 1 cut(s) 1358
AoxI GGCC 7 cut(s) 381, 441, 470, 809, 871, 969, 1374
ApaLI GTGCAC 1 cut(s) 1991
ApeKI GCWGC 5 cut(s) 338, 1099, 1967, 2309, 2447
ArsI GACNNNNNNTTYG 2 cut(s) 1828, 1860
AsiGI ACCGGT 1 cut(s) 696
Asp700I GAANNNNTTC 1 cut(s) 2205
AspA2I CCTAGG 1 cut(s) 1489
AspLEI GCGC 5 cut(s) 419, 985, 1676, 1706, 1783
AspS9I GGNCC 7 cut(s) 5, 872, 970, 1800, 1836, 1892, 1933
AsuC2I CCSGG 3 cut(s) 390, 391, 1960
AsuHPI GGTGA 4 cut(s) 92, 2101, 2360, 2361
AvaI CYCGRG 2 cut(s) 65, 389
AvaII GGWCC 5 cut(s) 5, 1800, 1836, 1892, 1933
AvrII CCTAGG 1 cut(s) 1489
AxyI CCTNAGG 1 cut(s) 625
BaeGI GKGCMC 2 cut(s) 380, 1995
BaeI ACNNNNGTAYC 2 cut(s) 947, 980
BamHI GGATCC 1 cut(s) 1521
BanI GGYRCC 1 cut(s) 416
BanII GRGCYC 1 cut(s) 396
BarI GAAGNNNNNNTAC 2 cut(s) 2321, 2353
BauI CACGAG 2 cut(s) 563, 593
BbsI GAAGAC 4 cut(s) 859, 1355, 1481, 1915
Bbv12I GWGCWC 3 cut(s) 759, 1509, 1995
BbvI GCAGC 5 cut(s) 325, 1111, 1954, 2321, 2459
BccI CCATC 5 cut(s) 142, 392, 430, 1163, 1883
BceAI ACGGC 9 cut(s) 392, 396, 428, 430, 1134, 1361, 1973, 1980, 2139
BciT130I CCWGG 3 cut(s) 477, 833, 1005
BclI TGATCA 2 cut(s) 163, 537
BcnI CCSGG 3 cut(s) 390, 391, 1960
BcoDI GTCTC 7 cut(s) 116, 213, 853, 1016, 1421, 2015, 2534
BcuI ACTAGT 1 cut(s) 2255
BfaI CTAG 8 cut(s) 354, 369, 1139, 1490, 1502, 1767, 2156, 2256
BfoI RGCGCY 2 cut(s) 420, 1784
BglI GCCNNNNNGGC 1 cut(s) 1964
BglII AGATCT 1 cut(s) 1465
BlnI CCTAGG 1 cut(s) 1489
BlpI GCTNAGC 1 cut(s) 1777
Bme1390I CCNGG 6 cut(s) 390, 391, 477, 833, 1005, 1960
Bme18I GGWCC 5 cut(s) 5, 1800, 1836, 1892, 1933
BmeT110I CYCGRG 2 cut(s) 65, 389
BmgT120I GGNCC 7 cut(s) 5, 872, 970, 1800, 1836, 1892, 1933
BmiI GGNNCC 7 cut(s) 6, 418, 450, 1523, 1683, 1837, 1934
BmrFI CCNGG 6 cut(s) 390, 391, 477, 833, 1005, 1960
BmrI ACTGGG 1 cut(s) 869
BmsI GCATC 4 cut(s) 276, 1545, 1572, 1594
BmuI ACTGGG 1 cut(s) 869
BoxI GACNNNNGTC 2 cut(s) 492, 1978
BpiI GAAGAC 4 cut(s) 859, 1355, 1481, 1915
BplI GAGNNNNNCTC 4 cut(s) 557, 589, 1013, 1045
BpmI CTGGAG 1 cut(s) 1017
Bpu10I CCTNAGC 1 cut(s) 1559
Bpu1102I GCTNAGC 1 cut(s) 1777
BpuEI CTTGAG 1 cut(s) 1690
BpuMI CCSGG 3 cut(s) 390, 391, 1960
Bsa29I ATCGAT 1 cut(s) 543
BsaBI GATNNNNATC 2 cut(s) 1526, 2065
BsaHI GRCGYC 4 cut(s) 417, 1044, 1953, 2055
BsaI GGTCTC 1 cut(s) 1421
BsaWI WCCGGW 4 cut(s) 696, 1358, 1802, 1935
BsaXI ACNNNNNCTCC 4 cut(s) 284, 314, 1015, 1045
Bsc4I CCNNNNNNNGG 9 cut(s) 77, 140, 373, 374, 390, 395, 979, 1607, 2081
Bse118I RCCGGY 1 cut(s) 696
Bse1I ACTGG 3 cut(s) 875, 1034, 2101
Bse21I CCTNAGG 1 cut(s) 625
Bse3DI GCAATG 1 cut(s) 1740
Bse8I GATNNNNATC 2 cut(s) 1526, 2065
BseAI TCCGGA 1 cut(s) 1358
BseBI CCWGG 3 cut(s) 477, 833, 1005
BseCI ATCGAT 1 cut(s) 543
BseGI GGATG 4 cut(s) 384, 441, 1114, 1585
BseJI GATNNNNATC 2 cut(s) 1526, 2065
BseLI CCNNNNNNNGG 9 cut(s) 77, 140, 373, 374, 390, 395, 979, 1607, 2081
BseMI GCAATG 1 cut(s) 1740
BseMII CTCAG 4 cut(s) 639, 1573, 1764, 1791
BseNI ACTGG 3 cut(s) 875, 1034, 2101
BseRI GAGGAG 1 cut(s) 1034
BseSI GKGCMC 2 cut(s) 380, 1995
BseXI GCAGC 5 cut(s) 325, 1111, 1954, 2321, 2459
Bsh1236I CGCG 2 cut(s) 1433, 1706
BshFI GGCC 7 cut(s) 383, 443, 472, 811, 873, 971, 1376
BshNI GGYRCC 1 cut(s) 416
BshTI ACCGGT 1 cut(s) 696
BshVI ATCGAT 1 cut(s) 543
BsiHKAI GWGCWC 3 cut(s) 759, 1509, 1995
BsiHKCI CYCGRG 2 cut(s) 65, 389
BsiSI CCGG 7 cut(s) 390, 697, 1359, 1803, 1936, 1959, 2058
BslFI GGGAC 6 cut(s) 182, 350, 1849, 1863, 1946, 1975
BslI CCNNNNNNNGG 9 cut(s) 77, 140, 373, 374, 390, 395, 979, 1607, 2081
BsmAI GTCTC 7 cut(s) 116, 213, 853, 1016, 1421, 2015, 2534
BsmFI GGGAC 6 cut(s) 182, 350, 1849, 1863, 1946, 1975
BsmI GAATGC 2 cut(s) 794, 1745
BsnI GGCC 7 cut(s) 383, 443, 472, 811, 873, 971, 1376
Bso31I GGTCTC 1 cut(s) 1421
BsoBI CYCGRG 2 cut(s) 65, 389
Bsp1286I GDGCHC 5 cut(s) 380, 396, 759, 1509, 1995
Bsp13I TCCGGA 1 cut(s) 1358
Bsp1720I GCTNAGC 1 cut(s) 1777
Bsp19I CCATGG 3 cut(s) 424, 612, 1947
BspANI GGCC 7 cut(s) 383, 443, 472, 811, 873, 971, 1376
BspCNI CTCAG 4 cut(s) 638, 1572, 1763, 1790
BspDI ATCGAT 1 cut(s) 543
BspEI TCCGGA 1 cut(s) 1358
BspFNI CGCG 2 cut(s) 1433, 1706
BspHI TCATGA 1 cut(s) 742
BspLI GGNNCC 7 cut(s) 6, 418, 450, 1523, 1683, 1837, 1934
BspPI GGATC 7 cut(s) 212, 1143, 1516, 1529, 1628, 1916, 2066
BspT107I GGYRCC 1 cut(s) 416
BspTNI GGTCTC 1 cut(s) 1421
BsrDI GCAATG 1 cut(s) 1740
BsrFI RCCGGY 1 cut(s) 696
BsrI ACTGG 3 cut(s) 875, 1034, 2101
BssAI RCCGGY 1 cut(s) 696
BssNAI GTATAC 1 cut(s) 1275
BssNI GRCGYC 4 cut(s) 417, 1044, 1953, 2055
BssSI CACGAG 2 cut(s) 563, 593
BssT1I CCWWGG 5 cut(s) 424, 612, 1489, 1928, 1947
Bst1107I GTATAC 1 cut(s) 1275
Bst2BI CACGAG 2 cut(s) 563, 593
Bst2UI CCWGG 3 cut(s) 477, 833, 1005
Bst4CI ACNGT 2 cut(s) 584, 1854
Bst6I CTCTTC 2 cut(s) 134, 1656
BstACI GRCGYC 4 cut(s) 417, 1044, 1953, 2055
BstAPI GCANNNNNTGC 1 cut(s) 101
BstC8I GCNNGC 3 cut(s) 973, 1995, 2513
BstDSI CCRYGG 7 cut(s) 424, 444, 612, 735, 1205, 1431, 1947
BstENI CCTNNNNNAGG 1 cut(s) 2079
BstF5I GGATG 4 cut(s) 384, 441, 1114, 1585
BstFNI CGCG 2 cut(s) 1433, 1706
BstH2I RGCGCY 2 cut(s) 420, 1784
BstHHI GCGC 5 cut(s) 419, 985, 1676, 1706, 1783
BstMAI GTCTC 7 cut(s) 116, 213, 853, 1016, 1421, 2015, 2534
BstNI CCWGG 3 cut(s) 477, 833, 1005
BstNSI RCATGY 5 cut(s) 493, 1099, 2292, 2498, 2515
BstPAI GACNNNNGTC 2 cut(s) 492, 1978
BstSCI CCNGG 6 cut(s) 388, 389, 475, 831, 1003, 1958
BstSLI GKGCMC 2 cut(s) 380, 1995
BstUI CGCG 2 cut(s) 1433, 1706
BstV1I GCAGC 5 cut(s) 325, 1111, 1954, 2321, 2459
BstV2I GAAGAC 4 cut(s) 859, 1355, 1481, 1915
BstX2I RGATCY 4 cut(s) 1135, 1465, 1521, 1633
BstXI CCANNNNNNTGG 1 cut(s) 1846
BstYI RGATCY 4 cut(s) 1135, 1465, 1521, 1633
BstZ17I GTATAC 1 cut(s) 1275
Bsu15I ATCGAT 1 cut(s) 543
Bsu36I CCTNAGG 1 cut(s) 625
BsuRI GGCC 7 cut(s) 383, 443, 472, 811, 873, 971, 1376
BsuTUI ATCGAT 1 cut(s) 543
BtgI CCRYGG 7 cut(s) 424, 444, 612, 735, 1205, 1431, 1947
BtsCI GGATG 4 cut(s) 384, 441, 1114, 1585
BtsIMutI CAGTG 1 cut(s) 1866
Cac8I GCNNGC 3 cut(s) 973, 1995, 2513
CciI TCATGA 1 cut(s) 742
CfoI GCGC 5 cut(s) 419, 985, 1676, 1706, 1783
Cfr10I RCCGGY 1 cut(s) 696
Cfr13I GGNCC 7 cut(s) 5, 872, 970, 1800, 1836, 1892, 1933
Cfr42I CCGCGG 1 cut(s) 1434
Cfr9I CCCGGG 1 cut(s) 389
ClaI ATCGAT 1 cut(s) 543
CseI GACGC 4 cut(s) 482, 1961, 2063, 2504
CsiI ACCWGGT 1 cut(s) 475
Csp6I GTAC 5 cut(s) 399, 619, 956, 1407, 1714
CspAI ACCGGT 1 cut(s) 696
CviQI GTAC 5 cut(s) 399, 619, 956, 1407, 1714
DinI GGCGCC 1 cut(s) 418
DraIII CACNNNGTG 1 cut(s) 1211
DrdI GACNNNNNNGTC 1 cut(s) 1664
DseDI GACNNNNNNGTC 1 cut(s) 1664
EaeI YGGCCR 2 cut(s) 381, 1374
Eam1104I CTCTTC 2 cut(s) 134, 1656
EarI CTCTTC 2 cut(s) 134, 1656
EciI GGCGGA 1 cut(s) 879
Eco130I CCWWGG 5 cut(s) 424, 612, 1489, 1928, 1947
Eco24I GRGCYC 1 cut(s) 396
Eco31I GGTCTC 1 cut(s) 1421
Eco32I GATATC 3 cut(s) 62, 1826, 2130
Eco47I GGWCC 5 cut(s) 5, 1800, 1836, 1892, 1933
Eco57I CTGAAG 1 cut(s) 251
Eco81I CCTNAGG 1 cut(s) 625
Eco88I CYCGRG 2 cut(s) 65, 389
EcoNI CCTNNNNNAGG 1 cut(s) 2079
EcoO109I RGGNCCY 1 cut(s) 5
EcoRII CCWGG 3 cut(s) 475, 831, 1003
EcoRV GATATC 3 cut(s) 62, 1826, 2130
EcoT14I CCWWGG 5 cut(s) 424, 612, 1489, 1928, 1947
EcoT22I ATGCAT 2 cut(s) 1587, 2294
EcoT38I GRGCYC 1 cut(s) 396
EgeI GGCGCC 1 cut(s) 418
EheI GGCGCC 1 cut(s) 418
ErhI CCWWGG 5 cut(s) 424, 612, 1489, 1928, 1947
FalI AAGNNNNNCTT 2 cut(s) 1802, 1834
FaqI GGGAC 6 cut(s) 182, 350, 1849, 1863, 1946, 1975
FauI CCCGC 1 cut(s) 980
FauNDI CATATG 1 cut(s) 1543
FbaI TGATCA 2 cut(s) 163, 537
FblI GTMKAC 4 cut(s) 411, 1274, 2141, 2480
FokI GGATG 4 cut(s) 371, 448, 1121, 1572
FriOI GRGCYC 1 cut(s) 396
FspBI CTAG 8 cut(s) 354, 369, 1139, 1490, 1502, 1767, 2156, 2256
GlaI GCGC 5 cut(s) 418, 984, 1675, 1705, 1782
GsuI CTGGAG 1 cut(s) 1017
HaeII RGCGCY 2 cut(s) 420, 1784
HaeIII GGCC 7 cut(s) 383, 443, 472, 811, 873, 971, 1376
HapII CCGG 7 cut(s) 390, 697, 1359, 1803, 1936, 1959, 2058
HgaI GACGC 4 cut(s) 482, 1961, 2063, 2504
HhaI GCGC 5 cut(s) 419, 985, 1676, 1706, 1783
Hin1I GRCGYC 4 cut(s) 417, 1044, 1953, 2055
Hin6I GCGC 5 cut(s) 417, 983, 1674, 1704, 1781
HinP1I GCGC 5 cut(s) 417, 983, 1674, 1704, 1781
HincII GTYRAC 4 cut(s) 412, 487, 639, 1756
HindII GTYRAC 4 cut(s) 412, 487, 639, 1756
HpaII CCGG 7 cut(s) 390, 697, 1359, 1803, 1936, 1959, 2058
HphI GGTGA 4 cut(s) 92, 2101, 2360, 2361
Hpy99I CGWCG 5 cut(s) 413, 416, 1661, 2057, 2520
HpyAV CCTTC 3 cut(s) 567, 1872, 2432
HpyCH4III ACNGT 2 cut(s) 584, 1854
HpyCH4IV ACGT 9 cut(s) 30, 160, 241, 1044, 1178, 1792, 2036, 2086, 2244
HpySE526I ACGT 9 cut(s) 30, 160, 241, 1044, 1178, 1792, 2036, 2086, 2244
Hsp92I GRCGYC 4 cut(s) 417, 1044, 1953, 2055
HspAI GCGC 5 cut(s) 417, 983, 1674, 1704, 1781
KasI GGCGCC 1 cut(s) 416
Kpn2I TCCGGA 1 cut(s) 1358
Ksp22I TGATCA 2 cut(s) 163, 537
KspI CCGCGG 1 cut(s) 1434
LmnI GCTCC 1 cut(s) 454
Lsp1109I GCAGC 5 cut(s) 325, 1111, 1954, 2321, 2459
LweI GCATC 4 cut(s) 276, 1545, 1572, 1594
MabI ACCWGGT 1 cut(s) 475
MaeI CTAG 8 cut(s) 354, 369, 1139, 1490, 1502, 1767, 2156, 2256
MaeII ACGT 9 cut(s) 30, 160, 241, 1044, 1178, 1792, 2036, 2086, 2244
MaeIII GTNAC 7 cut(s) 110, 316, 1114, 1179, 1193, 1286, 2336
MflI RGATCY 4 cut(s) 1135, 1465, 1521, 1633
MhlI GDGCHC 5 cut(s) 380, 396, 759, 1509, 1995
MluCI AATT 3 cut(s) 182, 686, 839
Mly113I GGCGCC 1 cut(s) 417
MlyI GAGTC 5 cut(s) 287, 839, 1471, 1521, 2146
MmeI TCCRAC 1 cut(s) 889
Mph1103I ATGCAT 2 cut(s) 1587, 2294
MroI TCCGGA 1 cut(s) 1358
MroXI GAANNNNTTC 1 cut(s) 2205
MseI TTAA 7 cut(s) 74, 1250, 1569, 1809, 1830, 2238, 2264
MslI CAYNNNNRTG 5 cut(s) 117, 714, 1109, 1601, 2417
MspA1I CMGCKG 2 cut(s) 338, 1433
MspI CCGG 7 cut(s) 390, 697, 1359, 1803, 1936, 1959, 2058
MspR9I CCNGG 6 cut(s) 390, 391, 477, 833, 1005, 1960
Mva1269I GAATGC 2 cut(s) 794, 1745
MvaI CCWGG 3 cut(s) 477, 833, 1005
MvnI CGCG 2 cut(s) 1433, 1706
NarI GGCGCC 1 cut(s) 417
NciI CCSGG 3 cut(s) 390, 391, 1960
NcoI CCATGG 3 cut(s) 424, 612, 1947
NdeI CATATG 1 cut(s) 1543
NlaIV GGNNCC 7 cut(s) 6, 418, 450, 1523, 1683, 1837, 1934
NmeAIII GCCGAG 3 cut(s) 918, 2044, 2206
NmuCI GTSAC 3 cut(s) 110, 316, 1286
NsiI ATGCAT 2 cut(s) 1587, 2294
NspI RCATGY 5 cut(s) 493, 1099, 2292, 2498, 2515
PaeI GCATGC 1 cut(s) 2515
PaeR7I CTCGAG 1 cut(s) 65
PagI TCATGA 1 cut(s) 742
PasI CCCWGGG 1 cut(s) 1004
PciI ACATGT 1 cut(s) 2494
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PctI GAATGC 2 cut(s) 794, 1745
PdmI GAANNNNTTC 1 cut(s) 2205
PfeI GAWTC 5 cut(s) 41, 553, 608, 1142, 1871
PinAI ACCGGT 1 cut(s) 696
PleI GAGTC 5 cut(s) 287, 839, 1471, 1520, 2146
PluTI GGCGCC 1 cut(s) 420
PpsI GAGTC 5 cut(s) 287, 839, 1471, 1520, 2146
PpuMI RGGWCCY 1 cut(s) 5
PscI ACATGT 1 cut(s) 2494
PshAI GACNNNNGTC 2 cut(s) 492, 1978
Psp5II RGGWCCY 1 cut(s) 5
Psp6I CCWGG 3 cut(s) 475, 831, 1003
PspGI CCWGG 3 cut(s) 475, 831, 1003
PspN4I GGNNCC 7 cut(s) 6, 418, 450, 1523, 1683, 1837, 1934
PspPI GGNCC 7 cut(s) 5, 872, 970, 1800, 1836, 1892, 1933
PspPPI RGGWCCY 1 cut(s) 5
PsuI RGATCY 4 cut(s) 1135, 1465, 1521, 1633
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 5 cut(s) 400, 620, 957, 1408, 1715
RsaNI GTAC 5 cut(s) 399, 619, 956, 1407, 1714
RseI CAYNNNNRTG 5 cut(s) 117, 714, 1109, 1601, 2417
SacII CCGCGG 1 cut(s) 1434
SalI GTCGAC 1 cut(s) 410
SaqAI TTAA 7 cut(s) 74, 1250, 1569, 1809, 1830, 2238, 2264
Sau96I GGNCC 7 cut(s) 5, 872, 970, 1800, 1836, 1892, 1933
SchI GAGTC 5 cut(s) 287, 839, 1471, 1521, 2146
ScrFI CCNGG 6 cut(s) 390, 391, 477, 833, 1005, 1960
SduI GDGCHC 5 cut(s) 380, 396, 759, 1509, 1995
SexAI ACCWGGT 1 cut(s) 475
SfaNI GCATC 4 cut(s) 276, 1545, 1572, 1594
SfoI GGCGCC 1 cut(s) 418
Sfr274I CTCGAG 1 cut(s) 65
Sfr303I CCGCGG 1 cut(s) 1434
SgrBI CCGCGG 1 cut(s) 1434
SgrDI CGTCGACG 1 cut(s) 410
SinI GGWCC 5 cut(s) 5, 1800, 1836, 1892, 1933
SlaI CTCGAG 1 cut(s) 65
SmaI CCCGGG 1 cut(s) 391
SmiMI CAYNNNNRTG 5 cut(s) 117, 714, 1109, 1601, 2417
SmlI CTYRAG 2 cut(s) 65, 1669
SmoI CTYRAG 2 cut(s) 65, 1669
SpeI ACTAGT 1 cut(s) 2255
SphI GCATGC 1 cut(s) 2515
Sse9I AATT 3 cut(s) 182, 686, 839
SspDI GGCGCC 1 cut(s) 416
SspMI CTAG 8 cut(s) 354, 369, 1139, 1490, 1502, 1767, 2156, 2256
StyD4I CCNGG 6 cut(s) 388, 389, 475, 831, 1003, 1958
StyI CCWWGG 5 cut(s) 424, 612, 1489, 1928, 1947
TaaI ACNGT 2 cut(s) 584, 1854
TaiI ACGT 9 cut(s) 33, 163, 244, 1047, 1181, 1795, 2039, 2089, 2247
TaqII GACCGA 1 cut(s) 527
TasI AATT 3 cut(s) 182, 686, 839
TatI WGTACW 1 cut(s) 1713
TauI GCSGC 6 cut(s) 407, 422, 1436, 1946, 1958, 2006
TfiI GAWTC 5 cut(s) 41, 553, 608, 1142, 1871
Tru1I TTAA 7 cut(s) 74, 1250, 1569, 1809, 1830, 2238, 2264
Tru9I TTAA 7 cut(s) 74, 1250, 1569, 1809, 1830, 2238, 2264
TscAI CASTG 1 cut(s) 1873
TseFI GTSAC 3 cut(s) 110, 316, 1286
TseI GCWGC 5 cut(s) 338, 1099, 1967, 2309, 2447
Tsp45I GTSAC 3 cut(s) 110, 316, 1286
TspGWI ACGGA 4 cut(s) 724, 1222, 1260, 1631
TspMI CCCGGG 1 cut(s) 389
TspRI CASTG 1 cut(s) 1873
VneI GTGCAC 1 cut(s) 1991
VpaK11BI GGWCC 5 cut(s) 5, 1800, 1836, 1892, 1933
XagI CCTNNNNNAGG 1 cut(s) 2079
XbaI TCTAGA 1 cut(s) 1138
XceI RCATGY 5 cut(s) 493, 1099, 2292, 2498, 2515
XcmI CCANNNNNNNNNTGG 1 cut(s) 2035
XhoI CTCGAG 1 cut(s) 65
XmaI CCCGGG 1 cut(s) 389
XmaJI CCTAGG 1 cut(s) 1489
XmiI GTMKAC 4 cut(s) 411, 1274, 2141, 2480
XmnI GAANNNNTTC 1 cut(s) 2205
XspI CTAG 8 cut(s) 354, 369, 1139, 1490, 1502, 1767, 2156, 2256
ZraI GACGTC 1 cut(s) 1045
Zsp2I ATGCAT 2 cut(s) 1587, 2294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.