aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 383, 516 |
| AccII | CGCG | 1 cut(s) | 522 |
| AciI | CCGC | 7 cut(s) | 58, 104, 223, 387, 408, 520, 522 |
| AdeI | CACNNNGTG | 1 cut(s) | 236 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 64, 77, 140, 373, 374, 375, 507, 508 |
| AjnI | CCWGG | 1 cut(s) | 485 |
| AluBI | AGCT | 2 cut(s) | 95, 338 |
| AluI | AGCT | 2 cut(s) | 95, 338 |
| Alw26I | GTCTC | 3 cut(s) | 7, 116, 213 |
| AoxI | GGCC | 2 cut(s) | 432, 460 |
| ApeKI | GCWGC | 1 cut(s) | 338 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 98, 130 |
| AseI | ATTAAT | 1 cut(s) | 20 |
| AspS9I | GGNCC | 1 cut(s) | 5 |
| AsuHPI | GGTGA | 1 cut(s) | 475 |
| AvaII | GGWCC | 1 cut(s) | 5 |
| BaeGI | GKGCMC | 2 cut(s) | 381, 514 |
| BanI | GGYRCC | 2 cut(s) | 383, 516 |
| BbsI | GAAGAC | 1 cut(s) | 121 |
| BbvI | GCAGC | 1 cut(s) | 325 |
| BceAI | ACGGC | 1 cut(s) | 419 |
| BciT130I | CCWGG | 1 cut(s) | 487 |
| BciVI | GTATCC | 1 cut(s) | 29 |
| BcoDI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BfaI | CTAG | 1 cut(s) | 96 |
| BfuI | GTATCC | 1 cut(s) | 29 |
| BisI | GCNGC | 3 cut(s) | 339, 387, 520 |
| BlsI | GCNGC | 3 cut(s) | 340, 388, 521 |
| Bme1390I | CCNGG | 1 cut(s) | 487 |
| Bme18I | GGWCC | 1 cut(s) | 5 |
| BmgT120I | GGNCC | 1 cut(s) | 5 |
| BmiI | GGNNCC | 5 cut(s) | 6, 69, 385, 441, 518 |
| BmrFI | CCNGG | 1 cut(s) | 487 |
| BmsI | GCATC | 1 cut(s) | 276 |
| BpiI | GAAGAC | 1 cut(s) | 121 |
| Bsa29I | ATCGAT | 1 cut(s) | 261 |
| BsaBI | GATNNNNATC | 1 cut(s) | 40 |
| BsaJI | CCNNGG | 5 cut(s) | 167, 435, 445, 486, 520 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 411, 441 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 64, 77, 140, 373, 374, 375, 507, 508 |
| Bse8I | GATNNNNATC | 1 cut(s) | 40 |
| BseBI | CCWGG | 1 cut(s) | 487 |
| BseCI | ATCGAT | 1 cut(s) | 261 |
| BseDI | CCNNGG | 5 cut(s) | 167, 435, 445, 486, 520 |
| BseJI | GATNNNNATC | 1 cut(s) | 40 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 64, 77, 140, 373, 374, 375, 507, 508 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BseSI | GKGCMC | 2 cut(s) | 381, 514 |
| BseXI | GCAGC | 1 cut(s) | 325 |
| Bsh1236I | CGCG | 1 cut(s) | 522 |
| BshFI | GGCC | 2 cut(s) | 434, 462 |
| BshNI | GGYRCC | 2 cut(s) | 383, 516 |
| BshVI | ATCGAT | 1 cut(s) | 261 |
| BslFI | GGGAC | 2 cut(s) | 182, 350 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 64, 77, 140, 373, 374, 375, 507, 508 |
| BsmAI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BsmBI | CGTCTC | 1 cut(s) | 7 |
| BsmFI | GGGAC | 2 cut(s) | 182, 350 |
| BsnI | GGCC | 2 cut(s) | 434, 462 |
| Bsp1286I | GDGCHC | 2 cut(s) | 381, 514 |
| Bsp143I | GATC | 3 cut(s) | 217, 262, 323 |
| BspACI | CCGC | 7 cut(s) | 58, 104, 223, 387, 408, 520, 522 |
| BspANI | GGCC | 2 cut(s) | 434, 462 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspDI | ATCGAT | 1 cut(s) | 261 |
| BspFNI | CGCG | 1 cut(s) | 522 |
| BspLI | GGNNCC | 5 cut(s) | 6, 69, 385, 441, 518 |
| BspT107I | GGYRCC | 2 cut(s) | 383, 516 |
| BssECI | CCNNGG | 5 cut(s) | 167, 435, 445, 486, 520 |
| BssMI | GATC | 3 cut(s) | 217, 262, 323 |
| BssT1I | CCWWGG | 2 cut(s) | 167, 445 |
| Bst2UI | CCWGG | 1 cut(s) | 487 |
| Bst4CI | ACNGT | 4 cut(s) | 110, 127, 383, 516 |
| Bst6I | CTCTTC | 1 cut(s) | 445 |
| BstC8I | GCNNGC | 1 cut(s) | 351 |
| BstDEI | CTNAG | 1 cut(s) | 9 |
| BstDSI | CCRYGG | 2 cut(s) | 435, 520 |
| BstFNI | CGCG | 1 cut(s) | 522 |
| BstKTI | GATC | 3 cut(s) | 220, 265, 326 |
| BstMAI | GTCTC | 3 cut(s) | 7, 116, 213 |
| BstMBI | GATC | 3 cut(s) | 217, 262, 323 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 101 |
| BstNI | CCWGG | 1 cut(s) | 487 |
| BstSCI | CCNGG | 1 cut(s) | 485 |
| BstSLI | GKGCMC | 2 cut(s) | 381, 514 |
| BstUI | CGCG | 1 cut(s) | 522 |
| BstV1I | GCAGC | 1 cut(s) | 325 |
| BstV2I | GAAGAC | 1 cut(s) | 121 |
| Bsu15I | ATCGAT | 1 cut(s) | 261 |
| BsuI | GTATCC | 1 cut(s) | 29 |
| BsuRI | GGCC | 2 cut(s) | 434, 462 |
| BsuTUI | ATCGAT | 1 cut(s) | 261 |
| BtgI | CCRYGG | 2 cut(s) | 435, 520 |
| BtsI | GCAGTG | 1 cut(s) | 399 |
| BtsIMutI | CAGTG | 1 cut(s) | 399 |
| Cac8I | GCNNGC | 1 cut(s) | 351 |
| Cfr13I | GGNCC | 1 cut(s) | 5 |
| Cfr42I | CCGCGG | 1 cut(s) | 523 |
| ClaI | ATCGAT | 1 cut(s) | 261 |
| CviJI | RGCY | 5 cut(s) | 95, 338, 434, 440, 462 |
| CviKI_1 | RGCY | 5 cut(s) | 95, 338, 434, 440, 462 |
| DdeI | CTNAG | 1 cut(s) | 9 |
| DpnI | GATC | 3 cut(s) | 219, 264, 325 |
| DpnII | GATC | 3 cut(s) | 217, 262, 323 |
| DraI | TTTAAA | 1 cut(s) | 91 |
| DraIII | CACNNNGTG | 1 cut(s) | 236 |
| Eam1104I | CTCTTC | 1 cut(s) | 445 |
| EarI | CTCTTC | 1 cut(s) | 445 |
| Eco130I | CCWWGG | 2 cut(s) | 167, 445 |
| Eco32I | GATATC | 1 cut(s) | 307 |
| Eco47I | GGWCC | 1 cut(s) | 5 |
| EcoO109I | RGGNCCY | 1 cut(s) | 5 |
| EcoRII | CCWGG | 1 cut(s) | 485 |
| EcoRV | GATATC | 1 cut(s) | 307 |
| EcoT14I | CCWWGG | 2 cut(s) | 167, 445 |
| ErhI | CCWWGG | 2 cut(s) | 167, 445 |
| Esp3I | CGTCTC | 1 cut(s) | 7 |
| FaiI | YATR | 5 cut(s) | 30, 275, 296, 330, 332 |
| FaqI | GGGAC | 2 cut(s) | 182, 350 |
| Fnu4HI | GCNGC | 3 cut(s) | 339, 387, 520 |
| Fsp4HI | GCNGC | 3 cut(s) | 339, 387, 520 |
| FspBI | CTAG | 1 cut(s) | 96 |
| GluI | GCNGC | 3 cut(s) | 339, 387, 520 |
| HaeIII | GGCC | 2 cut(s) | 434, 462 |
| HinfI | GANTC | 2 cut(s) | 41, 281 |
| HphI | GGTGA | 1 cut(s) | 475 |
| Hpy166II | GTNNAC | 1 cut(s) | 300 |
| Hpy188I | TCNGA | 2 cut(s) | 12, 270 |
| Hpy8I | GTNNAC | 1 cut(s) | 300 |
| HpyCH4III | ACNGT | 4 cut(s) | 110, 127, 383, 516 |
| HpyCH4V | TGCA | 3 cut(s) | 341, 353, 404 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 101 |
| HpyF3I | CTNAG | 1 cut(s) | 9 |
| KspI | CCGCGG | 1 cut(s) | 523 |
| Kzo9I | GATC | 3 cut(s) | 217, 262, 323 |
| LmnI | GCTCC | 1 cut(s) | 445 |
| LpnPI | CCDG | 7 cut(s) | 63, 164, 303, 348, 363, 472, 499 |
| Lsp1109I | GCAGC | 1 cut(s) | 325 |
| LweI | GCATC | 1 cut(s) | 276 |
| MaeI | CTAG | 1 cut(s) | 96 |
| MaeIII | GTNAC | 1 cut(s) | 110 |
| MalI | GATC | 3 cut(s) | 219, 264, 325 |
| MboI | GATC | 3 cut(s) | 217, 262, 323 |
| MboII | GAAGA | 3 cut(s) | 121, 257, 462 |
| MhlI | GDGCHC | 2 cut(s) | 381, 514 |
| MluCI | AATT | 2 cut(s) | 21, 182 |
| MmeI | TCCRAC | 2 cut(s) | 13, 71 |
| MnlI | CCTC | 6 cut(s) | 18, 389, 413, 424, 446, 452 |
| MseI | TTAA | 2 cut(s) | 20, 90 |
| MslI | CAYNNNNRTG | 1 cut(s) | 117 |
| MspA1I | CMGCKG | 2 cut(s) | 338, 522 |
| MspR9I | CCNGG | 1 cut(s) | 487 |
| MvaI | CCWGG | 1 cut(s) | 487 |
| MvnI | CGCG | 1 cut(s) | 522 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 101 |
| NdeII | GATC | 3 cut(s) | 217, 262, 323 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 69, 385, 441, 518 |
| NmuCI | GTSAC | 1 cut(s) | 110 |
| PfeI | GAWTC | 2 cut(s) | 41, 281 |
| PkrI | GCNGC | 3 cut(s) | 340, 388, 521 |
| PpuMI | RGGWCCY | 1 cut(s) | 5 |
| PshBI | ATTAAT | 1 cut(s) | 20 |
| Psp5II | RGGWCCY | 1 cut(s) | 5 |
| Psp6I | CCWGG | 1 cut(s) | 485 |
| PspGI | CCWGG | 1 cut(s) | 485 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 69, 385, 441, 518 |
| PspPI | GGNCC | 1 cut(s) | 5 |
| PspPPI | RGGWCCY | 1 cut(s) | 5 |
| PvuII | CAGCTG | 1 cut(s) | 338 |
| RseI | CAYNNNNRTG | 1 cut(s) | 117 |
| SacII | CCGCGG | 1 cut(s) | 523 |
| SaqAI | TTAA | 2 cut(s) | 20, 90 |
| SatI | GCNGC | 3 cut(s) | 339, 387, 520 |
| Sau3AI | GATC | 3 cut(s) | 217, 262, 323 |
| Sau96I | GGNCC | 1 cut(s) | 5 |
| ScrFI | CCNGG | 1 cut(s) | 487 |
| SduI | GDGCHC | 2 cut(s) | 381, 514 |
| SetI | ASST | 4 cut(s) | 97, 340, 359, 424 |
| SfaNI | GCATC | 1 cut(s) | 276 |
| Sfr303I | CCGCGG | 1 cut(s) | 523 |
| SgrBI | CCGCGG | 1 cut(s) | 523 |
| SinI | GGWCC | 1 cut(s) | 5 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 117 |
| Sse9I | AATT | 2 cut(s) | 21, 182 |
| SsiI | CCGC | 7 cut(s) | 58, 104, 223, 387, 408, 520, 522 |
| SspMI | CTAG | 1 cut(s) | 96 |
| StyD4I | CCNGG | 1 cut(s) | 485 |
| StyI | CCWWGG | 2 cut(s) | 167, 445 |
| TaaI | ACNGT | 4 cut(s) | 110, 127, 383, 516 |
| TaqI | TCGA | 3 cut(s) | 261, 284, 472 |
| TasI | AATT | 2 cut(s) | 21, 182 |
| TauI | GCSGC | 2 cut(s) | 389, 522 |
| TfiI | GAWTC | 2 cut(s) | 41, 281 |
| Tru1I | TTAA | 2 cut(s) | 20, 90 |
| Tru9I | TTAA | 2 cut(s) | 20, 90 |
| TscAI | CASTG | 1 cut(s) | 406 |
| TseFI | GTSAC | 1 cut(s) | 110 |
| TseI | GCWGC | 1 cut(s) | 338 |
| Tsp45I | GTSAC | 1 cut(s) | 110 |
| TspRI | CASTG | 1 cut(s) | 406 |
| VpaK11BI | GGWCC | 1 cut(s) | 5 |
| VspI | ATTAAT | 1 cut(s) | 20 |
| XspI | CTAG | 1 cut(s) | 96 |