Rmu_sc0035924.1_g000001

aspartic-type endopeptidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0035924.1
OG0000161
Physical Location & Seq
Forward (+)
1 .. 2121
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0035924.1_g000001.1.cds

Sequence Viewer

Length: 684 bp
aaggtagctgatactttacctgatgagttttctgaccttgctaaagtgacgagattacatatagctattgcaaatgtgcctacaaggatagacgtcccgactacaagacgtagcaccacccttgaagcaccacgggatgacgccactgcccctaagatctctgtaaactacacttgtgtacatcagaagtgggagagaaactccgtcatccttaatgacatattcgcgtactccgttgctcgtgagattattgagcctgatgacgtcgaacctcgctgtgttgatgaatgccaacgtagagtagattggccaaaatggaaaaatgtgatccaggaaaaattagattctctagcgaagaaggtatttgggcccgttgtgccagcattacctaacacaaacctgttggacataaatggtcttgaccctaaaaatgatccatttcgtcccaagaatgatggcgaagaagtgctagaggcagaagtgctctatctatgtgcaataggtgcattattgggtaagatagatatgagcttcttctatccctacaaagagaagatgaaggatgataatgatggattcagacccatcatgtaccaaaaccgcagccgccgccgcatctactgtcgacaggcgccatctttcccctcctcaaaacataaatggtgttttggaaggttttgttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_2 rve

Protein Analysis

227

Amino Acids

26.15

Weight (kDa)

8.15

Isoelectric Point (pI)

49.9

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000161)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g36192 FvH4_3g41702 FvH4_4g13933 FvH4_6g39331 FvH4_6g44542
malus_domestica MD17G1101700.v1.1
pyrus_communis pycom01g01810 pycom01g03340 pycom05g22610 pycom13g03220 pycom13g23770 pycom13g26870 pycom17g17400 pycom17g17410 pycom17g17890
rosa_chinensis RchiOBHm_Chr2g0129881 RchiOBHm_Chr2g0163801 RchiOBHm_Chr4g0392871 RchiOBHm_Chr7g0218471
rosa_multiflora Rmu_co8005914.1_g000001 Rmu_co8214666.1_g000002 Rmu_co8253889.1_g000001 Rmu_co8429419.1_g000001 Rmu_sc0000031.1_g000049 Rmu_sc0000091.1_g000004 Rmu_sc0000103.1_g000050 Rmu_sc0000147.1_g000047 Rmu_sc0000185.1_g000026 Rmu_sc0000235.1_g000056 Rmu_sc0000247.1_g000002 Rmu_sc0000310.1_g000010 Rmu_sc0000353.1_g000034 Rmu_sc0000353.1_g000035 Rmu_sc0000367.1_g000046 Rmu_sc0000420.1_g000034 Rmu_sc0000461.1_g000022 Rmu_sc0000544.1_g000007 Rmu_sc0000544.1_g000009 Rmu_sc0000607.1_g000032 Rmu_sc0000647.1_g000011 Rmu_sc0000675.1_g000049 Rmu_sc0000680.1_g000015 Rmu_sc0000795.1_g000112 Rmu_sc0000851.1_g000013 Rmu_sc0000868.1_g000019 Rmu_sc0000869.1_g000009 Rmu_sc0000898.1_g000061 Rmu_sc0000990.1_g000006 Rmu_sc0001009.1_g000006 Rmu_sc0001051.1_g000034 Rmu_sc0001067.1_g000005 Rmu_sc0001124.1_g000020 Rmu_sc0001142.1_g000016 Rmu_sc0001231.1_g000015 Rmu_sc0001235.1_g000013 Rmu_sc0001336.1_g000036 Rmu_sc0001552.1_g000033 Rmu_sc0001604.1_g000003 Rmu_sc0001605.1_g000009 Rmu_sc0001605.1_g000011 Rmu_sc0001668.1_g000025 Rmu_sc0001698.1_g000009 Rmu_sc0001721.1_g000010 Rmu_sc0001735.1_g000004 Rmu_sc0001832.1_g000017 Rmu_sc0001844.1_g000024 Rmu_sc0001880.1_g000026 Rmu_sc0001896.1_g000018 Rmu_sc0001918.1_g000011 Rmu_sc0002072.1_g000022 Rmu_sc0002109.1_g000003 Rmu_sc0002142.1_g000017 Rmu_sc0002166.1_g000001 Rmu_sc0002207.1_g000015 Rmu_sc0002346.1_g000001 Rmu_sc0002460.1_g000009 Rmu_sc0002460.1_g000043 Rmu_sc0002460.1_g000071 Rmu_sc0002672.1_g000001 Rmu_sc0002699.1_g000019 Rmu_sc0002736.1_g000007 Rmu_sc0002773.1_g000003 Rmu_sc0002893.1_g000006 Rmu_sc0002968.1_g000014 Rmu_sc0002968.1_g000015 Rmu_sc0003032.1_g000020 Rmu_sc0003044.1_g000024 Rmu_sc0003200.1_g000007 Rmu_sc0003261.1_g000031 Rmu_sc0003347.1_g000014 Rmu_sc0003385.1_g000019 Rmu_sc0003385.1_g000020 Rmu_sc0003419.1_g000017 Rmu_sc0003605.1_g000029 Rmu_sc0003679.1_g000010 Rmu_sc0003708.1_g000002 Rmu_sc0003737.1_g000033 Rmu_sc0003981.1_g000021 Rmu_sc0004101.1_g000009 Rmu_sc0004119.1_g000009 Rmu_sc0004248.1_g000021 Rmu_sc0004362.1_g000013 Rmu_sc0004520.1_g000020 Rmu_sc0004741.1_g000017 Rmu_sc0004771.1_g000007 Rmu_sc0004835.1_g000027 Rmu_sc0004969.1_g000010 Rmu_sc0005037.1_g000002 Rmu_sc0005272.1_g000006 Rmu_sc0005584.1_g000017 Rmu_sc0005652.1_g000005 Rmu_sc0005670.1_g000010 Rmu_sc0005781.1_g000035 Rmu_sc0005816.1_g000026 Rmu_sc0005852.1_g000003 Rmu_sc0006087.1_g000008 Rmu_sc0006152.1_g000005 Rmu_sc0006160.1_g000001 Rmu_sc0006313.1_g000014 Rmu_sc0006347.1_g000001 Rmu_sc0006583.1_g000024 Rmu_sc0006711.1_g000018 Rmu_sc0006952.1_g000003 Rmu_sc0006976.1_g000006 Rmu_sc0007115.1_g000006 Rmu_sc0007490.1_g000028 Rmu_sc0007740.1_g000003 Rmu_sc0007779.1_g000001 Rmu_sc0007907.1_g000003 Rmu_sc0007967.1_g000003 Rmu_sc0008456.1_g000006 Rmu_sc0008578.1_g000003 Rmu_sc0008642.1_g000001 Rmu_sc0008848.1_g000001 Rmu_sc0010065.1_g000009 Rmu_sc0010263.1_g000003 Rmu_sc0010632.1_g000004 Rmu_sc0011050.1_g000004 Rmu_sc0011563.1_g000005 Rmu_sc0011854.1_g000001 Rmu_sc0012216.1_g000003 Rmu_sc0012689.1_g000002 Rmu_sc0012945.1_g000001 Rmu_sc0013071.1_g000003 Rmu_sc0013305.1_g000008 Rmu_sc0013698.1_g000009 Rmu_sc0013772.1_g000007 Rmu_sc0013874.1_g000001 Rmu_sc0014217.1_g000001 Rmu_sc0014478.1_g000019 Rmu_sc0014724.1_g000002 Rmu_sc0016563.1_g000002 Rmu_sc0016563.1_g000003 Rmu_sc0017108.1_g000001 Rmu_sc0017408.1_g000002 Rmu_sc0020248.1_g000001 Rmu_sc0021416.1_g000005 Rmu_sc0022221.1_g000003 Rmu_sc0022402.1_g000002 Rmu_sc0023172.1_g000001 Rmu_sc0024783.1_g000001 Rmu_sc0028152.1_g000001 Rmu_sc0028541.1_g000001 Rmu_sc0029935.1_g000001 Rmu_sc0030041.1_g000001 Rmu_sc0035924.1_g000001 Rmu_sc0038113.1_g000002 Rmu_sc0040377.1_g000001 Rmu_ssc0000041.1_g000024 Rmu_ssc0000197.1_g000066 Rmu_ssc0000213.1_g000082 Rmu_ssc0000215.1_g000018
rosa_roxburghii Rroxscaffold_152G00434500 Rroxscaffold_3G00223490 Rroxscaffold_3G00226440 Rroxscaffold_3G00237960 Rroxscaffold_6G00394150
rosa_samantha Rh5CG379600 Rh5CG449200 Rh6DG448500
rosa_wichuraiana Rw0G004320 Rw1G010800 Rw2G031280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 96, 267
AccB1I GGYRCC 1 cut(s) 631
AccI GTMKAC 1 cut(s) 625
AccII CGCG 1 cut(s) 227
AciI CCGC 4 cut(s) 601, 607, 610, 613
AclWI GGATC 2 cut(s) 322, 428
AcoI YGGCCR 1 cut(s) 308
AcyI GRCGYC 4 cut(s) 93, 141, 264, 632
AfaI GTAC 3 cut(s) 180, 230, 593
AgsI TTSAA 1 cut(s) 125
AjnI CCWGG 1 cut(s) 330
AluBI AGCT 3 cut(s) 8, 65, 531
AluI AGCT 3 cut(s) 8, 65, 531
Alw21I GWGCWC 1 cut(s) 486
AlwI GGATC 2 cut(s) 322, 428
AoxI GGCC 2 cut(s) 308, 368
ApaI GGGCCC 1 cut(s) 372
ApeKI GCWGC 1 cut(s) 603
AspLEI GCGC 1 cut(s) 634
AspS9I GGNCC 2 cut(s) 368, 369
BaeGI GKGCMC 1 cut(s) 372
BalI TGGCCA 1 cut(s) 310
BanI GGYRCC 1 cut(s) 631
BanII GRGCYC 1 cut(s) 372
BauI CACGAG 1 cut(s) 240
Bbv12I GWGCWC 1 cut(s) 486
BbvI GCAGC 1 cut(s) 615
BccI CCATC 4 cut(s) 449, 566, 593, 643
BciT130I CCWGG 1 cut(s) 332
BfaI CTAG 2 cut(s) 350, 470
BfoI RGCGCY 1 cut(s) 635
BglII AGATCT 1 cut(s) 156
BisI GCNGC 4 cut(s) 604, 607, 610, 613
BlsI GCNGC 4 cut(s) 605, 608, 611, 614
Bme1390I CCNGG 1 cut(s) 332
BmgT120I GGNCC 2 cut(s) 368, 369
BmiI GGNNCC 2 cut(s) 370, 633
BmrFI CCNGG 1 cut(s) 332
BmsI GCATC 1 cut(s) 624
BplI GAGNNNNNCTC 2 cut(s) 185, 217
BsaHI GRCGYC 4 cut(s) 93, 141, 264, 632
BsaJI CCNNGG 1 cut(s) 131
BseBI CCWGG 1 cut(s) 332
BseDI CCNNGG 1 cut(s) 131
BseGI GGATG 3 cut(s) 142, 207, 568
BseRI GAGGAG 1 cut(s) 637
BseSI GKGCMC 1 cut(s) 372
BseXI GCAGC 1 cut(s) 615
Bsh1236I CGCG 1 cut(s) 227
BshFI GGCC 2 cut(s) 310, 370
BshNI GGYRCC 1 cut(s) 631
BsiHKAI GWGCWC 1 cut(s) 486
BslFI GGGAC 2 cut(s) 80, 429
BsmFI GGGAC 2 cut(s) 80, 429
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 2 cut(s) 310, 370
Bsp120I GGGCCC 1 cut(s) 368
Bsp1286I GDGCHC 2 cut(s) 372, 486
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 3 cut(s) 156, 327, 433
BspACI CCGC 4 cut(s) 601, 607, 610, 613
BspANI GGCC 2 cut(s) 310, 370
BspFNI CGCG 1 cut(s) 227
BspLI GGNNCC 2 cut(s) 370, 633
BspPI GGATC 2 cut(s) 322, 428
BspT107I GGYRCC 1 cut(s) 631
BsrGI TGTACA 1 cut(s) 178
BssECI CCNNGG 1 cut(s) 131
BssMI GATC 3 cut(s) 156, 327, 433
BssNI GRCGYC 4 cut(s) 93, 141, 264, 632
BssSI CACGAG 1 cut(s) 240
Bst2BI CACGAG 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 623
BstACI GRCGYC 4 cut(s) 93, 141, 264, 632
BstAPI GCANNNNNTGC 1 cut(s) 503
BstAUI TGTACA 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 1 cut(s) 153
BstDSI CCRYGG 1 cut(s) 131
BstF5I GGATG 3 cut(s) 142, 207, 568
BstFNI CGCG 1 cut(s) 227
BstH2I RGCGCY 1 cut(s) 635
BstHHI GCGC 1 cut(s) 634
BstKTI GATC 3 cut(s) 159, 330, 436
BstMBI GATC 3 cut(s) 156, 327, 433
BstMWI GCNNNNNNNGC 4 cut(s) 376, 503, 609, 612
BstNI CCWGG 1 cut(s) 332
BstSCI CCNGG 1 cut(s) 330
BstSLI GKGCMC 1 cut(s) 372
BstUI CGCG 1 cut(s) 227
BstV1I GCAGC 1 cut(s) 615
BstX2I RGATCY 1 cut(s) 156
BstYI RGATCY 1 cut(s) 156
BsuRI GGCC 2 cut(s) 310, 370
BtgI CCRYGG 1 cut(s) 131
BtsCI GGATG 3 cut(s) 142, 207, 568
BtsI GCAGTG 1 cut(s) 144
BtsIMutI CAGTG 1 cut(s) 144
Cac8I GCNNGC 1 cut(s) 381
CfoI GCGC 1 cut(s) 634
Cfr13I GGNCC 2 cut(s) 368, 369
CseI GACGC 1 cut(s) 149
Csp6I GTAC 3 cut(s) 179, 229, 592
CviAII CATG 1 cut(s) 589
CviJI RGCY 7 cut(s) 8, 65, 256, 310, 370, 531, 606
CviKI_1 RGCY 7 cut(s) 8, 65, 256, 310, 370, 531, 606
CviQI GTAC 3 cut(s) 179, 229, 592
DdeI CTNAG 1 cut(s) 153
DinI GGCGCC 1 cut(s) 633
DpnI GATC 3 cut(s) 158, 329, 435
DpnII GATC 3 cut(s) 156, 327, 433
EaeI YGGCCR 1 cut(s) 308
Eco24I GRGCYC 1 cut(s) 372
EcoRII CCWGG 1 cut(s) 330
EcoT38I GRGCYC 1 cut(s) 372
EgeI GGCGCC 1 cut(s) 633
EheI GGCGCC 1 cut(s) 633
FaeI CATG 1 cut(s) 592
FaiI YATR 8 cut(s) 60, 62, 221, 410, 493, 527, 590, 657
FaqI GGGAC 2 cut(s) 80, 429
FatI CATG 1 cut(s) 588
FblI GTMKAC 1 cut(s) 625
Fnu4HI GCNGC 4 cut(s) 604, 607, 610, 613
FokI GGATG 3 cut(s) 149, 194, 575
FriOI GRGCYC 1 cut(s) 372
Fsp4HI GCNGC 4 cut(s) 604, 607, 610, 613
FspBI CTAG 2 cut(s) 350, 470
GlaI GCGC 1 cut(s) 633
GluI GCNGC 4 cut(s) 604, 607, 610, 613
HaeII RGCGCY 1 cut(s) 635
HaeIII GGCC 2 cut(s) 310, 370
HgaI GACGC 1 cut(s) 149
HhaI GCGC 1 cut(s) 634
Hin1I GRCGYC 4 cut(s) 93, 141, 264, 632
Hin1II CATG 1 cut(s) 592
Hin6I GCGC 1 cut(s) 632
HinP1I GCGC 1 cut(s) 632
HincII GTYRAC 1 cut(s) 626
HindII GTYRAC 1 cut(s) 626
HinfI GANTC 2 cut(s) 344, 576
Hpy166II GTNNAC 3 cut(s) 166, 179, 626
Hpy188I TCNGA 3 cut(s) 34, 186, 581
Hpy188III TCNNGA 3 cut(s) 97, 242, 419
Hpy8I GTNNAC 3 cut(s) 166, 179, 626
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 3 cut(s) 352, 553, 666
HpyCH4III ACNGT 1 cut(s) 623
HpyCH4IV ACGT 4 cut(s) 93, 109, 264, 295
HpyCH4V TGCA 3 cut(s) 71, 497, 506
HpyF10VI GCNNNNNNNGC 4 cut(s) 376, 503, 609, 612
HpyF3I CTNAG 1 cut(s) 153
HpySE526I ACGT 4 cut(s) 93, 109, 264, 295
Hsp92I GRCGYC 4 cut(s) 93, 141, 264, 632
Hsp92II CATG 1 cut(s) 592
HspAI GCGC 1 cut(s) 632
KasI GGCGCC 1 cut(s) 631
Kzo9I GATC 3 cut(s) 156, 327, 433
LpnPI CCDG 7 cut(s) 33, 270, 317, 344, 393, 413, 614
Lsp1109I GCAGC 1 cut(s) 615
LweI GCATC 1 cut(s) 624
MaeI CTAG 2 cut(s) 350, 470
MaeII ACGT 4 cut(s) 93, 109, 264, 295
MaeIII GTNAC 1 cut(s) 46
MalI GATC 3 cut(s) 158, 329, 435
MboI GATC 3 cut(s) 156, 327, 433
MboII GAAGA 4 cut(s) 367, 473, 526, 565
MflI RGATCY 1 cut(s) 156
MhlI GDGCHC 2 cut(s) 372, 486
MlsI TGGCCA 1 cut(s) 310
MluCI AATT 1 cut(s) 338
MluNI TGGCCA 1 cut(s) 310
Mly113I GGCGCC 1 cut(s) 632
MmeI TCCRAC 1 cut(s) 384
MnlI CCTC 4 cut(s) 282, 466, 655, 658
Mox20I TGGCCA 1 cut(s) 310
MscI TGGCCA 1 cut(s) 310
MseI TTAA 2 cut(s) 213, 682
Msp20I TGGCCA 1 cut(s) 310
MspR9I CCNGG 1 cut(s) 332
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 1 cut(s) 332
MvnI CGCG 1 cut(s) 227
MwoI GCNNNNNNNGC 4 cut(s) 376, 503, 609, 612
NarI GGCGCC 1 cut(s) 632
NdeII GATC 3 cut(s) 156, 327, 433
NlaIII CATG 1 cut(s) 592
NlaIV GGNNCC 2 cut(s) 370, 633
NmuCI GTSAC 1 cut(s) 46
PcsI WCGNNNNNNNCGW 1 cut(s) 231
PctI GAATGC 1 cut(s) 293
PfeI GAWTC 2 cut(s) 344, 576
PfoI TCCNGGA 1 cut(s) 330
PkrI GCNGC 4 cut(s) 605, 608, 611, 614
PluTI GGCGCC 1 cut(s) 635
Psp6I CCWGG 1 cut(s) 330
PspGI CCWGG 1 cut(s) 330
PspN4I GGNNCC 2 cut(s) 370, 633
PspOMI GGGCCC 1 cut(s) 368
PspPI GGNCC 2 cut(s) 368, 369
PsuI RGATCY 1 cut(s) 156
RsaI GTAC 3 cut(s) 180, 230, 593
RsaNI GTAC 3 cut(s) 179, 229, 592
SalI GTCGAC 1 cut(s) 624
SaqAI TTAA 2 cut(s) 213, 682
SatI GCNGC 4 cut(s) 604, 607, 610, 613
Sau3AI GATC 3 cut(s) 156, 327, 433
Sau96I GGNCC 2 cut(s) 368, 369
ScrFI CCNGG 1 cut(s) 332
SduI GDGCHC 2 cut(s) 372, 486
SfaNI GCATC 1 cut(s) 624
SfoI GGCGCC 1 cut(s) 633
Sse9I AATT 1 cut(s) 338
SsiI CCGC 4 cut(s) 601, 607, 610, 613
SspDI GGCGCC 1 cut(s) 631
SspMI CTAG 2 cut(s) 350, 470
StyD4I CCNGG 1 cut(s) 330
TaaI ACNGT 1 cut(s) 623
TaiI ACGT 4 cut(s) 96, 112, 267, 298
TaqI TCGA 2 cut(s) 267, 625
TasI AATT 1 cut(s) 338
TatI WGTACW 1 cut(s) 178
TauI GCSGC 3 cut(s) 609, 612, 615
TfiI GAWTC 2 cut(s) 344, 576
Tru1I TTAA 2 cut(s) 213, 682
Tru9I TTAA 2 cut(s) 213, 682
TscAI CASTG 1 cut(s) 151
TseFI GTSAC 1 cut(s) 46
TseI GCWGC 1 cut(s) 603
Tsp45I GTSAC 1 cut(s) 46
TspDTI ATGAA 2 cut(s) 300, 572
TspGWI ACGGA 2 cut(s) 193, 223
TspRI CASTG 1 cut(s) 151
XmiI GTMKAC 1 cut(s) 625
XspI CTAG 2 cut(s) 350, 470
ZraI GACGTC 2 cut(s) 94, 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.