aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 2 cut(s) | 58, 223 |
| AclWI | GGATC | 1 cut(s) | 212 |
| AcuI | CTGAAG | 1 cut(s) | 251 |
| AdeI | CACNNNGTG | 2 cut(s) | 236, 415 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 64, 77, 372, 373, 374 |
| AgsI | TTSAA | 1 cut(s) | 473 |
| AluBI | AGCT | 2 cut(s) | 338, 598 |
| AluI | AGCT | 2 cut(s) | 338, 598 |
| Alw26I | GTCTC | 4 cut(s) | 7, 116, 213, 559 |
| AlwI | GGATC | 1 cut(s) | 212 |
| AoxI | GGCC | 2 cut(s) | 431, 460 |
| ApeKI | GCWGC | 2 cut(s) | 385, 595 |
| AspLEI | GCGC | 1 cut(s) | 611 |
| AspS9I | GGNCC | 1 cut(s) | 5 |
| AsuC2I | CCSGG | 1 cut(s) | 134 |
| AsuHPI | GGTGA | 1 cut(s) | 601 |
| AvaII | GGWCC | 1 cut(s) | 5 |
| BaeGI | GKGCMC | 1 cut(s) | 380 |
| BbsI | GAAGAC | 1 cut(s) | 121 |
| BbvI | GCAGC | 2 cut(s) | 372, 607 |
| BccI | CCATC | 1 cut(s) | 385 |
| BceAI | ACGGC | 1 cut(s) | 418 |
| BciVI | GTATCC | 1 cut(s) | 29 |
| BcnI | CCSGG | 1 cut(s) | 134 |
| BcoDI | GTCTC | 4 cut(s) | 7, 116, 213, 559 |
| BfaI | CTAG | 1 cut(s) | 96 |
| BfmI | CTRYAG | 1 cut(s) | 386 |
| BfuI | GTATCC | 1 cut(s) | 29 |
| BisI | GCNGC | 2 cut(s) | 386, 596 |
| BlsI | GCNGC | 2 cut(s) | 387, 597 |
| Bme1390I | CCNGG | 1 cut(s) | 134 |
| Bme18I | GGWCC | 1 cut(s) | 5 |
| BmgT120I | GGNCC | 1 cut(s) | 5 |
| BmiI | GGNNCC | 3 cut(s) | 6, 69, 440 |
| BmrFI | CCNGG | 1 cut(s) | 134 |
| BmsI | GCATC | 3 cut(s) | 276, 283, 582 |
| BpiI | GAAGAC | 1 cut(s) | 121 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 539, 571 |
| BpuMI | CCSGG | 1 cut(s) | 134 |
| Bsa29I | ATCGAT | 1 cut(s) | 261 |
| BsaJI | CCNNGG | 3 cut(s) | 167, 434, 487 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 410, 440 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 64, 77, 372, 373, 374 |
| Bse1I | ACTGG | 1 cut(s) | 591 |
| BseCI | ATCGAT | 1 cut(s) | 261 |
| BseDI | CCNNGG | 3 cut(s) | 167, 434, 487 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 64, 77, 372, 373, 374 |
| BseMII | CTCAG | 1 cut(s) | 23 |
| BseNI | ACTGG | 1 cut(s) | 591 |
| BseSI | GKGCMC | 1 cut(s) | 380 |
| BseXI | GCAGC | 2 cut(s) | 372, 607 |
| BshFI | GGCC | 2 cut(s) | 433, 462 |
| BshVI | ATCGAT | 1 cut(s) | 261 |
| BsiSI | CCGG | 1 cut(s) | 133 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 64, 77, 372, 373, 374 |
| BsmAI | GTCTC | 4 cut(s) | 7, 116, 213, 559 |
| BsmBI | CGTCTC | 1 cut(s) | 7 |
| BsnI | GGCC | 2 cut(s) | 433, 462 |
| Bsp1286I | GDGCHC | 1 cut(s) | 380 |
| Bsp143I | GATC | 4 cut(s) | 82, 217, 262, 323 |
| BspACI | CCGC | 2 cut(s) | 58, 223 |
| BspANI | GGCC | 2 cut(s) | 433, 462 |
| BspCNI | CTCAG | 1 cut(s) | 22 |
| BspDI | ATCGAT | 1 cut(s) | 261 |
| BspHI | TCATGA | 1 cut(s) | 544 |
| BspLI | GGNNCC | 3 cut(s) | 6, 69, 440 |
| BspMAI | CTGCAG | 1 cut(s) | 390 |
| BspPI | GGATC | 1 cut(s) | 212 |
| BsrI | ACTGG | 1 cut(s) | 591 |
| BssECI | CCNNGG | 3 cut(s) | 167, 434, 487 |
| BssMI | GATC | 4 cut(s) | 82, 217, 262, 323 |
| BssT1I | CCWWGG | 2 cut(s) | 167, 487 |
| Bst4CI | ACNGT | 2 cut(s) | 127, 382 |
| Bst6I | CTCTTC | 1 cut(s) | 445 |
| BstC8I | GCNNGC | 2 cut(s) | 351, 607 |
| BstDEI | CTNAG | 2 cut(s) | 9, 412 |
| BstDSI | CCRYGG | 1 cut(s) | 434 |
| BstHHI | GCGC | 1 cut(s) | 611 |
| BstKTI | GATC | 4 cut(s) | 85, 220, 265, 326 |
| BstMAI | GTCTC | 4 cut(s) | 7, 116, 213, 559 |
| BstMBI | GATC | 4 cut(s) | 82, 217, 262, 323 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 508 |
| BstSCI | CCNGG | 1 cut(s) | 132 |
| BstSFI | CTRYAG | 1 cut(s) | 386 |
| BstSLI | GKGCMC | 1 cut(s) | 380 |
| BstV1I | GCAGC | 2 cut(s) | 372, 607 |
| BstV2I | GAAGAC | 1 cut(s) | 121 |
| Bsu15I | ATCGAT | 1 cut(s) | 261 |
| BsuI | GTATCC | 1 cut(s) | 29 |
| BsuRI | GGCC | 2 cut(s) | 433, 462 |
| BsuTUI | ATCGAT | 1 cut(s) | 261 |
| BtgI | CCRYGG | 1 cut(s) | 434 |
| BtsI | GCAGTG | 1 cut(s) | 398 |
| BtsIMutI | CAGTG | 1 cut(s) | 398 |
| Cac8I | GCNNGC | 2 cut(s) | 351, 607 |
| CciI | TCATGA | 1 cut(s) | 544 |
| CfoI | GCGC | 1 cut(s) | 611 |
| Cfr13I | GGNCC | 1 cut(s) | 5 |
| ClaI | ATCGAT | 1 cut(s) | 261 |
| CviAII | CATG | 2 cut(s) | 545, 575 |
| CviJI | RGCY | 6 cut(s) | 338, 365, 433, 439, 462, 598 |
| CviKI_1 | RGCY | 6 cut(s) | 338, 365, 433, 439, 462, 598 |
| DdeI | CTNAG | 2 cut(s) | 9, 412 |
| DpnI | GATC | 4 cut(s) | 84, 219, 264, 325 |
| DpnII | GATC | 4 cut(s) | 82, 217, 262, 323 |
| DraIII | CACNNNGTG | 2 cut(s) | 236, 415 |
| Eam1104I | CTCTTC | 1 cut(s) | 445 |
| EarI | CTCTTC | 1 cut(s) | 445 |
| Eco130I | CCWWGG | 2 cut(s) | 167, 487 |
| Eco47I | GGWCC | 1 cut(s) | 5 |
| Eco57I | CTGAAG | 1 cut(s) | 251 |
| EcoO109I | RGGNCCY | 1 cut(s) | 5 |
| EcoT14I | CCWWGG | 2 cut(s) | 167, 487 |
| ErhI | CCWWGG | 2 cut(s) | 167, 487 |
| Esp3I | CGTCTC | 1 cut(s) | 7 |
| FaeI | CATG | 2 cut(s) | 548, 578 |
| FatI | CATG | 2 cut(s) | 544, 574 |
| FauI | CCCGC | 1 cut(s) | 230 |
| Fnu4HI | GCNGC | 2 cut(s) | 386, 596 |
| Fsp4HI | GCNGC | 2 cut(s) | 386, 596 |
| FspBI | CTAG | 1 cut(s) | 96 |
| GlaI | GCGC | 1 cut(s) | 610 |
| GluI | GCNGC | 2 cut(s) | 386, 596 |
| HaeIII | GGCC | 2 cut(s) | 433, 462 |
| HapII | CCGG | 1 cut(s) | 133 |
| HhaI | GCGC | 1 cut(s) | 611 |
| Hin1II | CATG | 2 cut(s) | 548, 578 |
| Hin6I | GCGC | 1 cut(s) | 609 |
| HinP1I | GCGC | 1 cut(s) | 609 |
| HinfI | GANTC | 2 cut(s) | 281, 578 |
| HpaII | CCGG | 1 cut(s) | 133 |
| HphI | GGTGA | 1 cut(s) | 601 |
| Hpy166II | GTNNAC | 1 cut(s) | 300 |
| Hpy188I | TCNGA | 3 cut(s) | 12, 270, 559 |
| Hpy188III | TCNNGA | 2 cut(s) | 96, 545 |
| Hpy8I | GTNNAC | 1 cut(s) | 300 |
| HpyAV | CCTTC | 1 cut(s) | 449 |
| HpyCH4III | ACNGT | 2 cut(s) | 127, 382 |
| HpyCH4IV | ACGT | 1 cut(s) | 273 |
| HpyCH4V | TGCA | 5 cut(s) | 311, 353, 388, 403, 595 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 508 |
| HpyF3I | CTNAG | 2 cut(s) | 9, 412 |
| HpySE526I | ACGT | 1 cut(s) | 273 |
| Hsp92II | CATG | 2 cut(s) | 548, 578 |
| HspAI | GCGC | 1 cut(s) | 609 |
| Kzo9I | GATC | 4 cut(s) | 82, 217, 262, 323 |
| LmnI | GCTCC | 1 cut(s) | 444 |
| LpnPI | CCDG | 7 cut(s) | 63, 146, 164, 303, 348, 363, 572 |
| Lsp1109I | GCAGC | 2 cut(s) | 372, 607 |
| LweI | GCATC | 3 cut(s) | 276, 283, 582 |
| MaeI | CTAG | 1 cut(s) | 96 |
| MaeII | ACGT | 1 cut(s) | 273 |
| MaeIII | GTNAC | 2 cut(s) | 110, 316 |
| MalI | GATC | 4 cut(s) | 84, 219, 264, 325 |
| MboI | GATC | 4 cut(s) | 82, 217, 262, 323 |
| MboII | GAAGA | 3 cut(s) | 121, 257, 462 |
| MfeI | CAATTG | 1 cut(s) | 473 |
| MhlI | GDGCHC | 1 cut(s) | 380 |
| MluCI | AATT | 3 cut(s) | 51, 182, 473 |
| MmeI | TCCRAC | 1 cut(s) | 13 |
| MnlI | CCTC | 9 cut(s) | 18, 103, 209, 213, 388, 423, 452, 491, 550 |
| MseI | TTAA | 1 cut(s) | 20 |
| MslI | CAYNNNNRTG | 1 cut(s) | 117 |
| MspA1I | CMGCKG | 1 cut(s) | 338 |
| MspI | CCGG | 1 cut(s) | 133 |
| MspR9I | CCNGG | 1 cut(s) | 134 |
| MunI | CAATTG | 1 cut(s) | 473 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 508 |
| NciI | CCSGG | 1 cut(s) | 134 |
| NdeII | GATC | 4 cut(s) | 82, 217, 262, 323 |
| NlaIII | CATG | 2 cut(s) | 548, 578 |
| NlaIV | GGNNCC | 3 cut(s) | 6, 69, 440 |
| NmuCI | GTSAC | 2 cut(s) | 110, 316 |
| PagI | TCATGA | 1 cut(s) | 544 |
| PfeI | GAWTC | 2 cut(s) | 281, 578 |
| PkrI | GCNGC | 2 cut(s) | 387, 597 |
| PpuMI | RGGWCCY | 1 cut(s) | 5 |
| Psp5II | RGGWCCY | 1 cut(s) | 5 |
| PspN4I | GGNNCC | 3 cut(s) | 6, 69, 440 |
| PspPI | GGNCC | 1 cut(s) | 5 |
| PspPPI | RGGWCCY | 1 cut(s) | 5 |
| PstI | CTGCAG | 1 cut(s) | 390 |
| PvuII | CAGCTG | 1 cut(s) | 338 |
| RseI | CAYNNNNRTG | 1 cut(s) | 117 |
| SaqAI | TTAA | 1 cut(s) | 20 |
| SatI | GCNGC | 2 cut(s) | 386, 596 |
| Sau3AI | GATC | 4 cut(s) | 82, 217, 262, 323 |
| Sau96I | GGNCC | 1 cut(s) | 5 |
| ScrFI | CCNGG | 1 cut(s) | 134 |
| SduI | GDGCHC | 1 cut(s) | 380 |
| SetI | ASST | 5 cut(s) | 95, 276, 340, 359, 600 |
| SfaNI | GCATC | 3 cut(s) | 276, 283, 582 |
| SfcI | CTRYAG | 1 cut(s) | 386 |
| SinI | GGWCC | 1 cut(s) | 5 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 117 |
| Sse9I | AATT | 3 cut(s) | 51, 182, 473 |
| SsiI | CCGC | 2 cut(s) | 58, 223 |
| SspMI | CTAG | 1 cut(s) | 96 |
| StyD4I | CCNGG | 1 cut(s) | 132 |
| StyI | CCWWGG | 2 cut(s) | 167, 487 |
| TaaI | ACNGT | 2 cut(s) | 127, 382 |
| TaiI | ACGT | 1 cut(s) | 276 |
| TaqI | TCGA | 2 cut(s) | 261, 284 |
| TasI | AATT | 3 cut(s) | 51, 182, 473 |
| TfiI | GAWTC | 2 cut(s) | 281, 578 |
| Tru1I | TTAA | 1 cut(s) | 20 |
| Tru9I | TTAA | 1 cut(s) | 20 |
| TscAI | CASTG | 1 cut(s) | 405 |
| TseFI | GTSAC | 2 cut(s) | 110, 316 |
| TseI | GCWGC | 2 cut(s) | 385, 595 |
| Tsp45I | GTSAC | 2 cut(s) | 110, 316 |
| TspDTI | ATGAA | 1 cut(s) | 591 |
| TspRI | CASTG | 1 cut(s) | 405 |
| VpaK11BI | GGWCC | 1 cut(s) | 5 |
| XbaI | TCTAGA | 1 cut(s) | 95 |
| XspI | CTAG | 1 cut(s) | 96 |