MD03G1110700.v1.1

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000289
Physical Location & Seq
Reverse (-)
9638617 .. 9639810
1194 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1110700.v1.1.491

Sequence Viewer

Length: 1194 bp
ATGGAATCGGATTCAGAGCTCCCGAGCGAAATTATTGGCTTTCAGATACTTTCGAGGATATCCACGAAGTCTCTGATGCGCTTCAAATCCGTATGCAAGTCTTGGTCATCTCTCATCCGCAACCCTTCCTTTGTCAATGCCCATCGGAAATTCTACCGCAACAAGCACAGTCACCTCCTCCTCACCACCTGGGACGAATCTGCAAGGCAGCAACACTTCTTTTCAGTAAAAATCAACCAAGAAGGAAGTCCAACTCTTGTCACCCACCTTCTAAAACTTCCAACACCGCCAGGCAAGAATGTTGAACGTCTCTACGAAGCACTGAGTATCAACGGCTTAGTCTGTCTTTACCTTTCTGACTCCTTGTTTCCCAATCAAACCGATCACAACCATCCTGTTCGAATATTCAATCCGTGCACCCGAGAGTGTATAGTTCTTCCTCATGATTCACCTGCGTACCGCACCATCCGTGTTACGCACCACTTTGGGTTCAGTTCTCATACGAACGAGTATAAGGTCCTTCAAGTGAAAAGGTTTCAGCCTCTAAATTCGACAAGGGACATGAGTTTCATGGTCAAGATTTTCAAACTAGGTACAAGTTCATGGAGACGGATAGAGGCAGACATTAACGATCTCCCATTTGATCCACTGAAGTGCCAGTTTGAAAGGAGAAGTGTGTGCGTCCATGGAGCCATACATTGGATGCATGACTCAACACAAAATATTATAGCGGTTTTCGACCTTAAAGATGAGAGATTCAGAGCCATCCCACTCCCTGAAGACTACGATTACAGAATTCATGGTATTAACGATAAGCTTGGGAACATATTTGAAATGGAAGGGTGTTTGGCTCTAATAGGTGACAAACAGTTGAGGCAGATGAATACGATGGAATTATGGATTTTGAAGGACTACCAAAACCAGGTGTGGGTTAAGGAAACTATCACCTTTCCGTTTAGTTGGACGGAGTTAGGGTACCCTGTTCCTTTCTATACAATTCACACCAGCGAGCTCCTCCTCCTCCAATCATCGAGATTACTCCGGCAAGATTCGTCAGAAGCGAGGGTGCTTTTTTACAATTTGAAGAGTAGAAGTTTTAGAAGTGAAAGTGTTTTTCCAGAATCGATACTTCCTAATGATATTCGGTTGAAGTTACTTGCTCATTATGATGACAGTATTGTCCCTTTAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_3

Protein Analysis

398

Amino Acids

46.45

Weight (kDa)

8.72

Isoelectric Point (pI)

56.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 44 2.3e-07 F-box domain
F-box-like PF12937 6 - 43 3e-06 F-box-like
FBA_3 PF08268 68 - 368 9.1e-36 F-box associated beta propeller domain
FBA_1 PF07734 73 - 312 2.7e-16 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000289)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12771 FvH4_1g24070 FvH4_1g24080 FvH4_1g24081 FvH4_3g05670 FvH4_3g33611 FvH4_3g43191 FvH4_4g07091 FvH4_4g07100 FvH4_4g28510 FvH4_4g28520 FvH4_4g30181 FvH4_4g30190 FvH4_4g34271 FvH4_4g34340 FvH4_5g31290 FvH4_6g37661 FvH4_7g33810
malus_domestica MD02G1067600.v1.1 MD03G1110700.v1.1 MD11G1124100.v1.1 MD11G1124200.v1.1 MD11G1124500.v1.1 MD13G1020600.v1.1 MD16G1019000.v1.1
prunus_persica Prupe.1G283900_v2.0.a1 Prupe.1G284000_v2.0.a1 Prupe.1G285200_v2.0.a1 Prupe.1G285300_v2.0.a1 Prupe.1G285500_v2.0.a1 Prupe.1G285600_v2.0.a1 Prupe.1G286300_v2.0.a1 Prupe.1G300600_v2.0.a1 Prupe.1G300900_v2.0.a1 Prupe.1G333100_v2.0.a1 Prupe.6G093100_v2.0.a1
pyrus_communis pycom02g05360 pycom04g05300 pycom11g10460 pycom11g10470 pycom11g10510 pycom13g01740 pycom13g01750 pycom13g04550
rosa_chinensis RchiOBHm_Chr2g0138411 RchiOBHm_Chr2g0138421 RchiOBHm_Chr4g0399251 RchiOBHm_Chr4g0436521
rosa_laevigata RLG00000005896 RLG00000005933 RLG00000006379 RLG00000006511 RLG00000009284 RLG00000019741 RLG00000019742 RLG00000019743 RLG00000020150 RLG00000024158 RLG00000024159 RLG00000030139 RLG00000032057
rosa_multiflora Rmu_co8310765.1_g000001 Rmu_co8476365.1_g000001 Rmu_sc0001426.1_g000004 Rmu_sc0001534.1_g000001 Rmu_sc0001534.1_g000009 Rmu_sc0001534.1_g000010 Rmu_sc0001556.1_g000019 Rmu_sc0001556.1_g000020 Rmu_sc0001779.1_g000020 Rmu_sc0003130.1_g000005 Rmu_sc0005961.1_g000007 Rmu_sc0025546.1_g000001 Rmu_sc0035131.1_g000001 Rmu_sc0035132.1_g000001
rosa_roxburghii Rroxscaffold_1G00062740 Rroxscaffold_2G00106090 Rroxscaffold_2G00106130 Rroxscaffold_2G00106210 Rroxscaffold_4G00324740 Rroxscaffold_4G00324760
rosa_rugosa Rorug04G0015700 Rorug04G0015800 Rorug04G0294500 Rorug04G0345900 Rorug04G0345900 Rorug05G0013800 Rorug05G0336100 Rorug05G0446400
rosa_samantha Rh2AG399300 Rh2BG407000 Rh2BG407300 Rh2BG407400 Rh2CG385800 Rh2DG419200 Rh4AG093400 Rh4AG348800 Rh4AG401200 Rh4AG406800 Rh4AG406900 Rh4CG101600 Rh4CG371900 Rh4CG428200 Rh4CG428300 Rh4DG085500 Rh4DG085700 Rh4DG351400 Rh4DG365000 Rh4DG408300 Rh4DG408400 Rh5AG108000 Rh5BG104700 Rh5BG411400 Rh5BG523400 Rh5DG103300 Rh5DG425800
rosa_wichuraiana Rw0G008940 Rw1G005620 Rw3G022740 Rw4G030510 Rw4G034940 Rw5G009440 Rw5G037570 Rw5G046580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 460
AasI GACNNNNNNGTC 1 cut(s) 1178
Acc36I ACCTGC 1 cut(s) 460
Acc65I GGTACC 1 cut(s) 975
AccB1I GGYRCC 1 cut(s) 975
AccB7I CCANNNNNTGG 1 cut(s) 699
AciI CCGC 5 cut(s) 118, 157, 287, 460, 731
AclWI GGATC 1 cut(s) 638
AcsI RAATTY 3 cut(s) 149, 547, 795
AcuI CTGAAG 2 cut(s) 671, 798
AfaI GTAC 3 cut(s) 458, 595, 977
AfiI CCNNNNNNNGG 3 cut(s) 699, 922, 928
AjnI CCWGG 3 cut(s) 188, 289, 921
AleI CACNNNNGTG 1 cut(s) 652
AluBI AGCT 3 cut(s) 19, 817, 1012
AluI AGCT 3 cut(s) 19, 817, 1012
Alw21I GWGCWC 3 cut(s) 21, 419, 1014
Alw26I GTCTC 3 cut(s) 75, 314, 601
Alw44I GTGCAC 1 cut(s) 415
AlwI GGATC 1 cut(s) 638
Ama87I CYCGRG 2 cut(s) 22, 420
ApaLI GTGCAC 1 cut(s) 415
ApeKI GCWGC 1 cut(s) 208
ApoI RAATTY 3 cut(s) 149, 547, 795
Asp718I GGTACC 1 cut(s) 975
AspLEI GCGC 1 cut(s) 81
AspS9I GGNCC 1 cut(s) 517
AsuHPI GGTGA 6 cut(s) 164, 175, 253, 441, 872, 937
AsuII TTCGAA 1 cut(s) 400
AvaI CYCGRG 2 cut(s) 22, 420
AvaII GGWCC 1 cut(s) 517
BaeGI GKGCMC 1 cut(s) 419
BaeI ACNNNNGTAYC 2 cut(s) 440, 473
BanI GGYRCC 1 cut(s) 975
BanII GRGCYC 2 cut(s) 21, 1014
BbsI GAAGAC 1 cut(s) 786
Bbv12I GWGCWC 3 cut(s) 21, 419, 1014
BbvI GCAGC 1 cut(s) 220
BccI CCATC 5 cut(s) 150, 399, 473, 773, 883
BceAI ACGGC 1 cut(s) 349
BciT130I CCWGG 3 cut(s) 190, 291, 923
BcoDI GTCTC 3 cut(s) 75, 314, 601
BfaI CTAG 1 cut(s) 590
BfuAI ACCTGC 1 cut(s) 460
BisI GCNGC 1 cut(s) 209
BlsI GCNGC 1 cut(s) 210
Bme1390I CCNGG 3 cut(s) 190, 291, 923
Bme18I GGWCC 1 cut(s) 517
BmeT110I CYCGRG 2 cut(s) 22, 420
BmgT120I GGNCC 1 cut(s) 517
BmiI GGNNCC 2 cut(s) 691, 977
BmrFI CCNGG 3 cut(s) 190, 291, 923
BmsI GCATC 2 cut(s) 66, 693
BpiI GAAGAC 1 cut(s) 786
Bpu14I TTCGAA 1 cut(s) 400
Bsa29I ATCGAT 1 cut(s) 1124
BsaJI CCNNGG 2 cut(s) 189, 685
BsaXI ACNNNNNCTCC 2 cut(s) 959, 989
Bsc4I CCNNNNNNNGG 3 cut(s) 699, 922, 928
Bse1I ACTGG 1 cut(s) 658
BseBI CCWGG 3 cut(s) 190, 291, 923
BseCI ATCGAT 1 cut(s) 1124
BseDI CCNNGG 2 cut(s) 189, 685
BseGI GGATG 5 cut(s) 114, 391, 465, 708, 765
BseLI CCNNNNNNNGG 3 cut(s) 699, 922, 928
BseMII CTCAG 1 cut(s) 314
BseNI ACTGG 1 cut(s) 658
BseRI GAGGAG 5 cut(s) 167, 170, 1004, 1007, 1010
BseSI GKGCMC 1 cut(s) 419
BseXI GCAGC 1 cut(s) 220
BshNI GGYRCC 1 cut(s) 975
BshVI ATCGAT 1 cut(s) 1124
BsiHKAI GWGCWC 3 cut(s) 21, 419, 1014
BsiHKCI CYCGRG 2 cut(s) 22, 420
BsiSI CCGG 1 cut(s) 1042
BslFI GGGAC 3 cut(s) 206, 572, 1166
BslI CCNNNNNNNGG 3 cut(s) 699, 922, 928
BsmAI GTCTC 3 cut(s) 75, 314, 601
BsmBI CGTCTC 2 cut(s) 314, 601
BsmFI GGGAC 3 cut(s) 206, 572, 1166
BsoBI CYCGRG 2 cut(s) 22, 420
Bsp119I TTCGAA 1 cut(s) 400
Bsp1286I GDGCHC 3 cut(s) 21, 419, 1014
Bsp143I GATC 3 cut(s) 382, 631, 643
Bsp19I CCATGG 1 cut(s) 685
BspACI CCGC 5 cut(s) 118, 157, 287, 460, 731
BspCNI CTCAG 1 cut(s) 315
BspDI ATCGAT 1 cut(s) 1124
BspHI TCATGA 1 cut(s) 442
BspLI GGNNCC 2 cut(s) 691, 977
BspMI ACCTGC 1 cut(s) 460
BspPI GGATC 1 cut(s) 638
BspT104I TTCGAA 1 cut(s) 400
BspT107I GGYRCC 1 cut(s) 975
BsrI ACTGG 1 cut(s) 658
BssECI CCNNGG 2 cut(s) 189, 685
BssMI GATC 3 cut(s) 382, 631, 643
BssT1I CCWWGG 1 cut(s) 685
Bst2UI CCWGG 3 cut(s) 190, 291, 923
Bst4CI ACNGT 3 cut(s) 170, 870, 1175
Bst6I CTCTTC 1 cut(s) 1079
BstBI TTCGAA 1 cut(s) 400
BstC8I GCNNGC 1 cut(s) 1010
BstDEI CTNAG 2 cut(s) 323, 337
BstDSI CCRYGG 1 cut(s) 685
BstF5I GGATG 5 cut(s) 114, 391, 465, 708, 765
BstHHI GCGC 1 cut(s) 81
BstKTI GATC 3 cut(s) 385, 634, 646
BstMAI GTCTC 3 cut(s) 75, 314, 601
BstMBI GATC 3 cut(s) 382, 631, 643
BstNI CCWGG 3 cut(s) 190, 291, 923
BstSCI CCNGG 3 cut(s) 188, 289, 921
BstSLI GKGCMC 1 cut(s) 419
BstV1I GCAGC 1 cut(s) 220
BstV2I GAAGAC 1 cut(s) 786
Bsu15I ATCGAT 1 cut(s) 1124
BsuTUI ATCGAT 1 cut(s) 1124
BtgI CCRYGG 1 cut(s) 685
BtsCI GGATG 5 cut(s) 114, 391, 465, 708, 765
BtsIMutI CAGTG 2 cut(s) 320, 647
BveI ACCTGC 1 cut(s) 460
Cac8I GCNNGC 1 cut(s) 1010
CciI TCATGA 1 cut(s) 442
CfoI GCGC 1 cut(s) 81
Cfr13I GGNCC 1 cut(s) 517
ClaI ATCGAT 1 cut(s) 1124
CseI GACGC 1 cut(s) 670
CsiI ACCWGGT 1 cut(s) 921
Csp6I GTAC 3 cut(s) 457, 594, 976
CviAII CATG 7 cut(s) 443, 562, 571, 603, 686, 707, 800
CviJI RGCY 9 cut(s) 19, 39, 336, 541, 692, 764, 817, 851, 1012
CviKI_1 RGCY 9 cut(s) 19, 39, 336, 541, 692, 764, 817, 851, 1012
CviQI GTAC 3 cut(s) 457, 594, 976
DdeI CTNAG 2 cut(s) 323, 337
DpnI GATC 3 cut(s) 384, 633, 645
DpnII GATC 3 cut(s) 382, 631, 643
DrdI GACNNNNNNGTC 1 cut(s) 1178
DseDI GACNNNNNNGTC 1 cut(s) 1178
Eam1104I CTCTTC 1 cut(s) 1079
EarI CTCTTC 1 cut(s) 1079
Ecl136II GAGCTC 2 cut(s) 19, 1012
Eco130I CCWWGG 1 cut(s) 685
Eco24I GRGCYC 2 cut(s) 21, 1014
Eco32I GATATC 1 cut(s) 60
Eco47I GGWCC 1 cut(s) 517
Eco53kI GAGCTC 2 cut(s) 19, 1012
Eco57I CTGAAG 2 cut(s) 671, 798
Eco88I CYCGRG 2 cut(s) 22, 420
EcoICRI GAGCTC 2 cut(s) 19, 1012
EcoO109I RGGNCCY 1 cut(s) 517
EcoRI GAATTC 1 cut(s) 795
EcoRII CCWGG 3 cut(s) 188, 289, 921
EcoRV GATATC 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 685
EcoT22I ATGCAT 1 cut(s) 708
EcoT38I GRGCYC 2 cut(s) 21, 1014
ErhI CCWWGG 1 cut(s) 685
Esp3I CGTCTC 2 cut(s) 314, 601
FaeI CATG 7 cut(s) 446, 565, 574, 606, 689, 710, 803
FaqI GGGAC 3 cut(s) 206, 572, 1166
FatI CATG 7 cut(s) 442, 561, 570, 602, 685, 706, 799
Fnu4HI GCNGC 1 cut(s) 209
FokI GGATG 5 cut(s) 101, 378, 452, 715, 752
FriOI GRGCYC 2 cut(s) 21, 1014
Fsp4HI GCNGC 1 cut(s) 209
FspBI CTAG 1 cut(s) 590
GlaI GCGC 1 cut(s) 80
GluI GCNGC 1 cut(s) 209
HapII CCGG 1 cut(s) 1042
HgaI GACGC 1 cut(s) 670
HhaI GCGC 1 cut(s) 81
Hin1II CATG 7 cut(s) 446, 565, 574, 606, 689, 710, 803
Hin6I GCGC 1 cut(s) 79
HinP1I GCGC 1 cut(s) 79
HindIII AAGCTT 1 cut(s) 815
HinfI GANTC 9 cut(s) 5, 11, 197, 359, 446, 710, 756, 1049, 1121
HpaII CCGG 1 cut(s) 1042
HphI GGTGA 6 cut(s) 164, 175, 253, 441, 872, 937
Hpy166II GTNNAC 1 cut(s) 417
Hpy188I TCNGA 8 cut(s) 10, 16, 45, 75, 147, 358, 761, 1057
Hpy188III TCNNGA 5 cut(s) 22, 443, 577, 1032, 1118
Hpy8I GTNNAC 1 cut(s) 417
HpyAV CCTTC 6 cut(s) 135, 236, 278, 530, 833, 901
HpyCH4III ACNGT 3 cut(s) 170, 870, 1175
HpyCH4IV ACGT 1 cut(s) 307
HpyCH4V TGCA 4 cut(s) 96, 203, 417, 706
HpyF3I CTNAG 2 cut(s) 323, 337
HpySE526I ACGT 1 cut(s) 307
Hsp92II CATG 7 cut(s) 446, 565, 574, 606, 689, 710, 803
HspAI GCGC 1 cut(s) 79
KpnI GGTACC 1 cut(s) 979
Kzo9I GATC 3 cut(s) 382, 631, 643
LmnI GCTCC 3 cut(s) 24, 689, 1017
Lsp1109I GCAGC 1 cut(s) 220
LweI GCATC 2 cut(s) 66, 693
MabI ACCWGGT 1 cut(s) 921
MaeI CTAG 1 cut(s) 590
MaeII ACGT 1 cut(s) 307
MaeIII GTNAC 5 cut(s) 170, 259, 472, 860, 1152
MalI GATC 3 cut(s) 384, 633, 645
MboI GATC 3 cut(s) 382, 631, 643
MboII GAAGA 3 cut(s) 428, 791, 1096
MhlI GDGCHC 3 cut(s) 21, 419, 1014
MluCI AATT 7 cut(s) 30, 149, 547, 795, 893, 996, 1078
MlyI GAGTC 2 cut(s) 353, 704
MmeI TCCRAC 3 cut(s) 275, 305, 941
Mph1103I ATGCAT 1 cut(s) 708
MseI TTAA 5 cut(s) 627, 744, 807, 933, 1187
MslI CAYNNNNRTG 2 cut(s) 652, 1167
MspI CCGG 1 cut(s) 1042
MspR9I CCNGG 3 cut(s) 190, 291, 923
MvaI CCWGG 3 cut(s) 190, 291, 923
NcoI CCATGG 1 cut(s) 685
NdeII GATC 3 cut(s) 382, 631, 643
NlaIII CATG 7 cut(s) 446, 565, 574, 606, 689, 710, 803
NlaIV GGNNCC 2 cut(s) 691, 977
NmuCI GTSAC 3 cut(s) 170, 259, 860
NsiI ATGCAT 1 cut(s) 708
NspV TTCGAA 1 cut(s) 400
OliI CACNNNNGTG 1 cut(s) 652
PagI TCATGA 1 cut(s) 442
PaqCI CACCTGC 1 cut(s) 460
PcsI WCGNNNNNNNCGW 1 cut(s) 1058
PfeI GAWTC 7 cut(s) 5, 11, 197, 446, 756, 1049, 1121
PflMI CCANNNNNTGG 1 cut(s) 699
PkrI GCNGC 1 cut(s) 210
PleI GAGTC 2 cut(s) 353, 704
PpsI GAGTC 2 cut(s) 353, 704
PpuMI RGGWCCY 1 cut(s) 517
Psp124BI GAGCTC 2 cut(s) 21, 1014
Psp5II RGGWCCY 1 cut(s) 517
Psp6I CCWGG 3 cut(s) 188, 289, 921
PspGI CCWGG 3 cut(s) 188, 289, 921
PspN4I GGNNCC 2 cut(s) 691, 977
PspPI GGNCC 1 cut(s) 517
PspPPI RGGWCCY 1 cut(s) 517
RsaI GTAC 3 cut(s) 458, 595, 977
RsaNI GTAC 3 cut(s) 457, 594, 976
RseI CAYNNNNRTG 2 cut(s) 652, 1167
SacI GAGCTC 2 cut(s) 21, 1014
SaqAI TTAA 5 cut(s) 627, 744, 807, 933, 1187
SatI GCNGC 1 cut(s) 209
Sau3AI GATC 3 cut(s) 382, 631, 643
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 2 cut(s) 353, 704
ScrFI CCNGG 3 cut(s) 190, 291, 923
SduI GDGCHC 3 cut(s) 21, 419, 1014
SexAI ACCWGGT 1 cut(s) 921
SfaNI GCATC 2 cut(s) 66, 693
SfuI TTCGAA 1 cut(s) 400
SinI GGWCC 1 cut(s) 517
SmiMI CAYNNNNRTG 2 cut(s) 652, 1167
Sse9I AATT 7 cut(s) 30, 149, 547, 795, 893, 996, 1078
SsiI CCGC 5 cut(s) 118, 157, 287, 460, 731
SspI AATATT 2 cut(s) 405, 724
SspMI CTAG 1 cut(s) 590
SstI GAGCTC 2 cut(s) 21, 1014
StyD4I CCNGG 3 cut(s) 188, 289, 921
StyI CCWWGG 1 cut(s) 685
TaaI ACNGT 3 cut(s) 170, 870, 1175
TaiI ACGT 1 cut(s) 310
TaqI TCGA 6 cut(s) 53, 400, 551, 738, 1031, 1124
TasI AATT 7 cut(s) 30, 149, 547, 795, 893, 996, 1078
TfiI GAWTC 7 cut(s) 5, 11, 197, 446, 756, 1049, 1121
Tru1I TTAA 5 cut(s) 627, 744, 807, 933, 1187
Tru9I TTAA 5 cut(s) 627, 744, 807, 933, 1187
TscAI CASTG 2 cut(s) 327, 654
TseFI GTSAC 3 cut(s) 170, 259, 860
TseI GCWGC 1 cut(s) 208
Tsp45I GTSAC 3 cut(s) 170, 259, 860
TspDTI ATGAA 4 cut(s) 559, 591, 788, 896
TspGWI ACGGA 6 cut(s) 79, 402, 458, 625, 942, 980
TspRI CASTG 2 cut(s) 327, 654
Van91I CCANNNNNTGG 1 cut(s) 699
VneI GTGCAC 1 cut(s) 415
VpaK11BI GGWCC 1 cut(s) 517
XapI RAATTY 3 cut(s) 149, 547, 795
XspI CTAG 1 cut(s) 590
Zsp2I ATGCAT 1 cut(s) 708
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.