Belongs to the NPH3 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1135 |
| AciI | CCGC | 4 cut(s) | 143, 625, 768, 778 |
| AclWI | GGATC | 1 cut(s) | 1123 |
| AcsI | RAATTY | 1 cut(s) | 1105 |
| AcuI | CTGAAG | 4 cut(s) | 182, 222, 927, 1138 |
| AfaI | GTAC | 3 cut(s) | 716, 836, 1186 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 142, 472, 727, 847 |
| AflII | CTTAAG | 1 cut(s) | 989 |
| AflIII | ACRYGT | 1 cut(s) | 230 |
| AgsI | TTSAA | 9 cut(s) | 120, 247, 401, 511, 758, 886, 920, 1004, 1110 |
| AjnI | CCWGG | 3 cut(s) | 49, 720, 981 |
| AleI | CACNNNNGTG | 1 cut(s) | 689 |
| AluBI | AGCT | 6 cut(s) | 59, 192, 563, 791, 964, 988 |
| AluI | AGCT | 6 cut(s) | 59, 192, 563, 791, 964, 988 |
| Alw21I | GWGCWC | 2 cut(s) | 538, 1206 |
| Alw26I | GTCTC | 1 cut(s) | 346 |
| Alw44I | GTGCAC | 2 cut(s) | 534, 1202 |
| AlwI | GGATC | 1 cut(s) | 1123 |
| ApaLI | GTGCAC | 2 cut(s) | 534, 1202 |
| ApeKI | GCWGC | 2 cut(s) | 476, 923 |
| ApoI | RAATTY | 1 cut(s) | 1105 |
| AspA2I | CCTAGG | 1 cut(s) | 1217 |
| AspLEI | GCGC | 1 cut(s) | 209 |
| AspS9I | GGNCC | 2 cut(s) | 104, 157 |
| AsuHPI | GGTGA | 3 cut(s) | 9, 15, 653 |
| AvaII | GGWCC | 2 cut(s) | 104, 157 |
| AvrII | CCTAGG | 1 cut(s) | 1217 |
| BaeGI | GKGCMC | 2 cut(s) | 538, 1206 |
| Bbv12I | GWGCWC | 2 cut(s) | 538, 1206 |
| BbvI | GCAGC | 2 cut(s) | 463, 935 |
| BccI | CCATC | 3 cut(s) | 28, 56, 585 |
| BceAI | ACGGC | 1 cut(s) | 918 |
| BciT130I | CCWGG | 3 cut(s) | 51, 722, 983 |
| BclI | TGATCA | 1 cut(s) | 1041 |
| BcoDI | GTCTC | 1 cut(s) | 346 |
| BfaI | CTAG | 2 cut(s) | 786, 1218 |
| BfmI | CTRYAG | 3 cut(s) | 477, 555, 830 |
| BfrI | CTTAAG | 1 cut(s) | 989 |
| BisI | GCNGC | 4 cut(s) | 144, 477, 768, 924 |
| BlnI | CCTAGG | 1 cut(s) | 1217 |
| BlsI | GCNGC | 4 cut(s) | 145, 478, 769, 925 |
| Bme1390I | CCNGG | 3 cut(s) | 51, 722, 983 |
| Bme18I | GGWCC | 2 cut(s) | 104, 157 |
| BmgT120I | GGNCC | 2 cut(s) | 104, 157 |
| BmrFI | CCNGG | 3 cut(s) | 51, 722, 983 |
| BmrI | ACTGGG | 1 cut(s) | 1184 |
| BmsI | GCATC | 1 cut(s) | 1186 |
| BmuI | ACTGGG | 1 cut(s) | 1184 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 93, 125 |
| BpuEI | CTTGAG | 3 cut(s) | 509, 539, 1052 |
| BsaBI | GATNNNNATC | 2 cut(s) | 663, 801 |
| BsaJI | CCNNGG | 2 cut(s) | 50, 1217 |
| BsaWI | WCCGGW | 2 cut(s) | 1017, 1240 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 142, 472, 727, 847 |
| Bse1I | ACTGG | 1 cut(s) | 1179 |
| Bse3DI | GCAATG | 2 cut(s) | 250, 392 |
| Bse8I | GATNNNNATC | 2 cut(s) | 663, 801 |
| BseBI | CCWGG | 3 cut(s) | 51, 722, 983 |
| BseDI | CCNNGG | 2 cut(s) | 50, 1217 |
| BseGI | GGATG | 4 cut(s) | 172, 802, 1000, 1201 |
| BseJI | GATNNNNATC | 2 cut(s) | 663, 801 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 142, 472, 727, 847 |
| BseMI | GCAATG | 2 cut(s) | 250, 392 |
| BseMII | CTCAG | 3 cut(s) | 361, 786, 1254 |
| BseNI | ACTGG | 1 cut(s) | 1179 |
| BseRI | GAGGAG | 2 cut(s) | 1246, 1249 |
| BseSI | GKGCMC | 2 cut(s) | 538, 1206 |
| BseXI | GCAGC | 2 cut(s) | 463, 935 |
| BsgI | GTGCAG | 1 cut(s) | 255 |
| BsiHKAI | GWGCWC | 2 cut(s) | 538, 1206 |
| BsiSI | CCGG | 2 cut(s) | 1018, 1241 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 142, 472, 727, 847 |
| BsmAI | GTCTC | 1 cut(s) | 346 |
| Bsp1286I | GDGCHC | 2 cut(s) | 538, 1206 |
| Bsp143I | GATC | 2 cut(s) | 1041, 1115 |
| BspACI | CCGC | 4 cut(s) | 143, 625, 768, 778 |
| BspCNI | CTCAG | 3 cut(s) | 360, 785, 1255 |
| BspHI | TCATGA | 1 cut(s) | 544 |
| BspMAI | CTGCAG | 2 cut(s) | 481, 559 |
| BspPI | GGATC | 1 cut(s) | 1123 |
| BspQI | GCTCTTC | 1 cut(s) | 999 |
| BspTI | CTTAAG | 1 cut(s) | 989 |
| BsrDI | GCAATG | 2 cut(s) | 250, 392 |
| BsrI | ACTGG | 1 cut(s) | 1179 |
| BssECI | CCNNGG | 2 cut(s) | 50, 1217 |
| BssMI | GATC | 2 cut(s) | 1041, 1115 |
| BssT1I | CCWWGG | 1 cut(s) | 1217 |
| Bst2UI | CCWGG | 3 cut(s) | 51, 722, 983 |
| Bst4CI | ACNGT | 5 cut(s) | 213, 598, 688, 834, 955 |
| Bst6I | CTCTTC | 2 cut(s) | 639, 999 |
| BstAFI | CTTAAG | 1 cut(s) | 989 |
| BstC8I | GCNNGC | 1 cut(s) | 629 |
| BstDEI | CTNAG | 6 cut(s) | 347, 384, 772, 1073, 1140, 1263 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 725 |
| BstF5I | GGATG | 4 cut(s) | 172, 802, 1000, 1201 |
| BstHHI | GCGC | 1 cut(s) | 209 |
| BstKTI | GATC | 2 cut(s) | 1044, 1118 |
| BstMAI | GTCTC | 1 cut(s) | 346 |
| BstMBI | GATC | 2 cut(s) | 1041, 1115 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 389, 462 |
| BstNI | CCWGG | 3 cut(s) | 51, 722, 983 |
| BstNSI | RCATGY | 1 cut(s) | 234 |
| BstSCI | CCNGG | 3 cut(s) | 49, 720, 981 |
| BstSFI | CTRYAG | 3 cut(s) | 477, 555, 830 |
| BstSLI | GKGCMC | 2 cut(s) | 538, 1206 |
| BstV1I | GCAGC | 2 cut(s) | 463, 935 |
| BtgZI | GCGATG | 2 cut(s) | 270, 587 |
| BtsCI | GGATG | 4 cut(s) | 172, 802, 1000, 1201 |
| BtsI | GCAGTG | 3 cut(s) | 111, 1105, 1206 |
| BtsIMutI | CAGTG | 5 cut(s) | 111, 209, 750, 1105, 1206 |
| Cac8I | GCNNGC | 1 cut(s) | 629 |
| CciI | TCATGA | 1 cut(s) | 544 |
| CfoI | GCGC | 1 cut(s) | 209 |
| Cfr13I | GGNCC | 2 cut(s) | 104, 157 |
| Csp6I | GTAC | 3 cut(s) | 715, 835, 1185 |
| CviAII | CATG | 7 cut(s) | 231, 329, 545, 632, 782, 1045, 1135 |
| CviQI | GTAC | 3 cut(s) | 715, 835, 1185 |
| DdeI | CTNAG | 6 cut(s) | 347, 384, 772, 1073, 1140, 1263 |
| DpnI | GATC | 2 cut(s) | 1043, 1117 |
| DpnII | GATC | 2 cut(s) | 1041, 1115 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1135 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1135 |
| Eam1104I | CTCTTC | 2 cut(s) | 639, 999 |
| EarI | CTCTTC | 2 cut(s) | 639, 999 |
| EciI | GGCGGA | 1 cut(s) | 767 |
| Eco130I | CCWWGG | 1 cut(s) | 1217 |
| Eco47I | GGWCC | 2 cut(s) | 104, 157 |
| Eco57I | CTGAAG | 4 cut(s) | 182, 222, 927, 1138 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 725 |
| EcoRI | GAATTC | 1 cut(s) | 1105 |
| EcoRII | CCWGG | 3 cut(s) | 49, 720, 981 |
| EcoT14I | CCWWGG | 1 cut(s) | 1217 |
| ErhI | CCWWGG | 1 cut(s) | 1217 |
| FaeI | CATG | 7 cut(s) | 234, 332, 548, 635, 785, 1048, 1138 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 381, 413 |
| FatI | CATG | 7 cut(s) | 230, 328, 544, 631, 781, 1044, 1134 |
| FauNDI | CATATG | 1 cut(s) | 264 |
| FbaI | TGATCA | 1 cut(s) | 1041 |
| Fnu4HI | GCNGC | 4 cut(s) | 144, 477, 768, 924 |
| FokI | GGATG | 4 cut(s) | 179, 809, 1007, 1208 |
| Fsp4HI | GCNGC | 4 cut(s) | 144, 477, 768, 924 |
| FspBI | CTAG | 2 cut(s) | 786, 1218 |
| GlaI | GCGC | 1 cut(s) | 208 |
| GluI | GCNGC | 4 cut(s) | 144, 477, 768, 924 |
| HapII | CCGG | 2 cut(s) | 1018, 1241 |
| HhaI | GCGC | 1 cut(s) | 209 |
| Hin1II | CATG | 7 cut(s) | 234, 332, 548, 635, 785, 1048, 1138 |
| Hin6I | GCGC | 1 cut(s) | 207 |
| HinP1I | GCGC | 1 cut(s) | 207 |
| HincII | GTYRAC | 2 cut(s) | 437, 613 |
| HindII | GTYRAC | 2 cut(s) | 437, 613 |
| HindIII | AAGCTT | 1 cut(s) | 561 |
| HinfI | GANTC | 8 cut(s) | 96, 268, 323, 424, 460, 916, 1069, 1253 |
| HpaII | CCGG | 2 cut(s) | 1018, 1241 |
| HphI | GGTGA | 3 cut(s) | 9, 15, 653 |
| Hpy166II | GTNNAC | 6 cut(s) | 107, 437, 536, 613, 1128, 1204 |
| Hpy188I | TCNGA | 6 cut(s) | 10, 101, 202, 294, 915, 1264 |
| Hpy8I | GTNNAC | 6 cut(s) | 107, 437, 536, 613, 1128, 1204 |
| HpyAV | CCTTC | 4 cut(s) | 157, 197, 359, 1219 |
| HpyCH4III | ACNGT | 5 cut(s) | 213, 598, 688, 834, 955 |
| HpyCH4V | TGCA | 8 cut(s) | 236, 299, 479, 536, 557, 1171, 1199, 1204 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 389, 462 |
| HpyF3I | CTNAG | 6 cut(s) | 347, 384, 772, 1073, 1140, 1263 |
| Hsp92II | CATG | 7 cut(s) | 234, 332, 548, 635, 785, 1048, 1138 |
| HspAI | GCGC | 1 cut(s) | 207 |
| Ksp22I | TGATCA | 1 cut(s) | 1041 |
| Kzo9I | GATC | 2 cut(s) | 1041, 1115 |
| LguI | GCTCTTC | 1 cut(s) | 999 |
| LmnI | GCTCC | 3 cut(s) | 56, 415, 985 |
| Lsp1109I | GCAGC | 2 cut(s) | 463, 935 |
| LweI | GCATC | 1 cut(s) | 1186 |
| MaeI | CTAG | 2 cut(s) | 786, 1218 |
| MalI | GATC | 2 cut(s) | 1043, 1117 |
| MboI | GATC | 2 cut(s) | 1041, 1115 |
| MboII | GAAGA | 3 cut(s) | 533, 656, 1016 |
| MhlI | GDGCHC | 2 cut(s) | 538, 1206 |
| MluCI | AATT | 9 cut(s) | 35, 174, 736, 752, 761, 886, 937, 1105, 1145 |
| MlyI | GAGTC | 3 cut(s) | 317, 433, 1247 |
| MseI | TTAA | 2 cut(s) | 450, 990 |
| MslI | CAYNNNNRTG | 6 cut(s) | 255, 304, 543, 636, 689, 1049 |
| MspCI | CTTAAG | 1 cut(s) | 989 |
| MspI | CCGG | 2 cut(s) | 1018, 1241 |
| MspR9I | CCNGG | 3 cut(s) | 51, 722, 983 |
| MvaI | CCWGG | 3 cut(s) | 51, 722, 983 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 389, 462 |
| NdeI | CATATG | 1 cut(s) | 264 |
| NdeII | GATC | 2 cut(s) | 1041, 1115 |
| NlaIII | CATG | 7 cut(s) | 234, 332, 548, 635, 785, 1048, 1138 |
| NspI | RCATGY | 1 cut(s) | 234 |
| OliI | CACNNNNGTG | 1 cut(s) | 689 |
| PagI | TCATGA | 1 cut(s) | 544 |
| PciI | ACATGT | 1 cut(s) | 230 |
| PciSI | GCTCTTC | 1 cut(s) | 999 |
| PfeI | GAWTC | 5 cut(s) | 96, 268, 460, 916, 1069 |
| PfoI | TCCNGGA | 1 cut(s) | 720 |
| PkrI | GCNGC | 4 cut(s) | 145, 478, 769, 925 |
| PleI | GAGTC | 3 cut(s) | 317, 432, 1247 |
| PpsI | GAGTC | 3 cut(s) | 317, 432, 1247 |
| PscI | ACATGT | 1 cut(s) | 230 |
| Psp6I | CCWGG | 3 cut(s) | 49, 720, 981 |
| PspGI | CCWGG | 3 cut(s) | 49, 720, 981 |
| PspPI | GGNCC | 2 cut(s) | 104, 157 |
| PstI | CTGCAG | 2 cut(s) | 481, 559 |
| RsaI | GTAC | 3 cut(s) | 716, 836, 1186 |
| RsaNI | GTAC | 3 cut(s) | 715, 835, 1185 |
| RseI | CAYNNNNRTG | 6 cut(s) | 255, 304, 543, 636, 689, 1049 |
| SapI | GCTCTTC | 1 cut(s) | 999 |
| SaqAI | TTAA | 2 cut(s) | 450, 990 |
| SatI | GCNGC | 4 cut(s) | 144, 477, 768, 924 |
| Sau3AI | GATC | 2 cut(s) | 1041, 1115 |
| Sau96I | GGNCC | 2 cut(s) | 104, 157 |
| SchI | GAGTC | 3 cut(s) | 317, 433, 1247 |
| ScrFI | CCNGG | 3 cut(s) | 51, 722, 983 |
| SduI | GDGCHC | 2 cut(s) | 538, 1206 |
| SfaNI | GCATC | 1 cut(s) | 1186 |
| SfcI | CTRYAG | 3 cut(s) | 477, 555, 830 |
| SinI | GGWCC | 2 cut(s) | 104, 157 |
| SmiMI | CAYNNNNRTG | 6 cut(s) | 255, 304, 543, 636, 689, 1049 |
| SmlI | CTYRAG | 4 cut(s) | 488, 518, 989, 1031 |
| SmoI | CTYRAG | 4 cut(s) | 488, 518, 989, 1031 |
| Sse9I | AATT | 9 cut(s) | 35, 174, 736, 752, 761, 886, 937, 1105, 1145 |
| SsiI | CCGC | 4 cut(s) | 143, 625, 768, 778 |
| SspMI | CTAG | 2 cut(s) | 786, 1218 |
| StyD4I | CCNGG | 3 cut(s) | 49, 720, 981 |
| StyI | CCWWGG | 1 cut(s) | 1217 |
| TaaI | ACNGT | 5 cut(s) | 213, 598, 688, 834, 955 |
| TaqI | TCGA | 1 cut(s) | 271 |
| TasI | AATT | 9 cut(s) | 35, 174, 736, 752, 761, 886, 937, 1105, 1145 |
| TauI | GCSGC | 2 cut(s) | 146, 770 |
| TfiI | GAWTC | 5 cut(s) | 96, 268, 460, 916, 1069 |
| Tru1I | TTAA | 2 cut(s) | 450, 990 |
| Tru9I | TTAA | 2 cut(s) | 450, 990 |
| TscAI | CASTG | 5 cut(s) | 118, 216, 750, 1105, 1206 |
| TseI | GCWGC | 2 cut(s) | 476, 923 |
| TspDTI | ATGAA | 7 cut(s) | 183, 533, 561, 590, 1011, 1067, 1071 |
| TspRI | CASTG | 5 cut(s) | 118, 216, 750, 1105, 1206 |
| Vha464I | CTTAAG | 1 cut(s) | 989 |
| VneI | GTGCAC | 2 cut(s) | 534, 1202 |
| VpaK11BI | GGWCC | 2 cut(s) | 104, 157 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 725 |
| XapI | RAATTY | 1 cut(s) | 1105 |
| XceI | RCATGY | 1 cut(s) | 234 |
| XmaJI | CCTAGG | 1 cut(s) | 1217 |
| XspI | CTAG | 2 cut(s) | 786, 1218 |