Rh4DG085700

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000289
Physical Location & Seq
Reverse (-)
13058084 .. 13059079
996 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG085700.1

Sequence Viewer

Length: 996 bp
ATGCAAGAAGATGCAGAGGTCCCTAACTTTCTGGAATACCTAGCAGCAAACCTCTTGGTCTCCTTTTTGCCTTCAATAACTCGCCTCTCTACCTCTCCGCTGCGAAACCCTAAAAAAATAAAAGCACATGATGCAGAATCACTCCCAACTGATCTCATATGGTTTGATATCCTCCCAAGGCTACCGCCCGAGTCCTTGATGCGCATTAGGTGCAAGTCTTGGTCTACTCTAACCCGAAACCCTAGTTTTGTCAGCGCCTACCGTAACTTCCACCGCGACAGCGACAGACAATCTCACCTCCTCCTCATCACTTGGGAAAAAGCAACAAAGCAGCACCACCTCTTCTCCTCCGAAATCAATCAAGAGGACGAAACTCCAACACCCACCACTCATCACCTTTTAACTTTGTCCTCCACTACATGTGATATCTATGGAGCACAAAGCATCAATGGCTTGGTCTGTTTTCACCTTTCTAATTACATGGCTGTTTCCAATAACATGGACCGCCCCGACCACCCAGTTCAAATATTGAATCCCACAACCCTAGAGTGCATTACTATCCCGTATTACTCACCCAGCTACCCCACATCCTATGTTACATACCACTTCGGGTTCAATCCTCTTGCAGACGAGTATAAGATTCTCCAAGTCCAACGGTTGGAAAAAGATTACAGGGGTATGAGTAGTTGGATGTATCAGATTTTTACCCTTGGAAAAAGTTCATGGAGGCAAATTGAGGAAGACTTTGACCTTAATCATCTAGTCCCTTTTGATTCTCACGAGTGCCACTTCCGTAAGGGTGGTTTGTATGCCAATGGGGTCATGTATTGGTTGACACGGGACATGATAGTGGTTTTTGAATTTGTACGTGAGAAATTCAGAGTGATGCCACTTCCACAAGGTAGTCGTCTTTATTCTCGTCCGAAGCTGTTTGAAATGGGCGGATGCTTGGCTATAATACGGCTTCAAGAGAAAGGGTTAGAGTTATCGATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box F-box-like FBA_3

Protein Analysis

331

Amino Acids

38.51

Weight (kDa)

6.5

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 101 - 325 1.4e-25 F-box associated beta propeller domain
FBA_1 PF07734 113 - 302 4.2e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000289)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12771 FvH4_1g24070 FvH4_1g24080 FvH4_1g24081 FvH4_3g05670 FvH4_3g33611 FvH4_3g43191 FvH4_4g07091 FvH4_4g07100 FvH4_4g28510 FvH4_4g28520 FvH4_4g30181 FvH4_4g30190 FvH4_4g34271 FvH4_4g34340 FvH4_5g31290 FvH4_6g37661 FvH4_7g33810
malus_domestica MD02G1067600.v1.1 MD03G1110700.v1.1 MD11G1124100.v1.1 MD11G1124200.v1.1 MD11G1124500.v1.1 MD13G1020600.v1.1 MD16G1019000.v1.1
prunus_persica Prupe.1G283900_v2.0.a1 Prupe.1G284000_v2.0.a1 Prupe.1G285200_v2.0.a1 Prupe.1G285300_v2.0.a1 Prupe.1G285500_v2.0.a1 Prupe.1G285600_v2.0.a1 Prupe.1G286300_v2.0.a1 Prupe.1G300600_v2.0.a1 Prupe.1G300900_v2.0.a1 Prupe.1G333100_v2.0.a1 Prupe.6G093100_v2.0.a1
pyrus_communis pycom02g05360 pycom04g05300 pycom11g10460 pycom11g10470 pycom11g10510 pycom13g01740 pycom13g01750 pycom13g04550
rosa_chinensis RchiOBHm_Chr2g0138411 RchiOBHm_Chr2g0138421 RchiOBHm_Chr4g0399251 RchiOBHm_Chr4g0436521
rosa_laevigata RLG00000005896 RLG00000005933 RLG00000006379 RLG00000006511 RLG00000009284 RLG00000019741 RLG00000019742 RLG00000019743 RLG00000020150 RLG00000024158 RLG00000024159 RLG00000030139 RLG00000032057
rosa_multiflora Rmu_co8310765.1_g000001 Rmu_co8476365.1_g000001 Rmu_sc0001426.1_g000004 Rmu_sc0001534.1_g000001 Rmu_sc0001534.1_g000009 Rmu_sc0001534.1_g000010 Rmu_sc0001556.1_g000019 Rmu_sc0001556.1_g000020 Rmu_sc0001779.1_g000020 Rmu_sc0003130.1_g000005 Rmu_sc0005961.1_g000007 Rmu_sc0025546.1_g000001 Rmu_sc0035131.1_g000001 Rmu_sc0035132.1_g000001
rosa_roxburghii Rroxscaffold_1G00062740 Rroxscaffold_2G00106090 Rroxscaffold_2G00106130 Rroxscaffold_2G00106210 Rroxscaffold_4G00324740 Rroxscaffold_4G00324760
rosa_rugosa Rorug04G0015700 Rorug04G0015800 Rorug04G0294500 Rorug04G0345900 Rorug04G0345900 Rorug05G0013800 Rorug05G0336100 Rorug05G0446400
rosa_samantha Rh2AG399300 Rh2BG407000 Rh2BG407300 Rh2BG407400 Rh2CG385800 Rh2DG419200 Rh4AG093400 Rh4AG348800 Rh4AG401200 Rh4AG406800 Rh4AG406900 Rh4CG101600 Rh4CG371900 Rh4CG428200 Rh4CG428300 Rh4DG085500 Rh4DG085700 Rh4DG351400 Rh4DG365000 Rh4DG408300 Rh4DG408400 Rh5AG108000 Rh5BG104700 Rh5BG411400 Rh5BG523400 Rh5DG103300 Rh5DG425800
rosa_wichuraiana Rw0G008940 Rw1G005620 Rw3G022740 Rw4G030510 Rw4G034940 Rw5G009440 Rw5G037570 Rw5G046580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 203
AccB7I CCANNNNNTGG 1 cut(s) 658
AccI GTMKAC 1 cut(s) 224
AccII CGCG 1 cut(s) 276
AciI CCGC 5 cut(s) 98, 185, 274, 505, 942
AcsI RAATTY 2 cut(s) 860, 875
AfaI GTAC 1 cut(s) 867
AfiI CCNNNNNNNGG 1 cut(s) 658
AflIII ACRYGT 1 cut(s) 419
AgsI TTSAA 7 cut(s) 75, 524, 532, 616, 860, 935, 968
AluBI AGCT 2 cut(s) 579, 928
AluI AGCT 2 cut(s) 579, 928
Alw21I GWGCWC 1 cut(s) 439
Alw26I GTCTC 1 cut(s) 64
Ama87I CYCGRG 1 cut(s) 188
ApeKI GCWGC 3 cut(s) 44, 100, 331
ApoI RAATTY 2 cut(s) 860, 875
Asp700I GAANNNNTTC 1 cut(s) 718
AspLEI GCGC 2 cut(s) 204, 257
AspS9I GGNCC 2 cut(s) 19, 502
AsuHPI GGTGA 4 cut(s) 287, 386, 458, 564
AvaI CYCGRG 1 cut(s) 188
AvaII GGWCC 2 cut(s) 19, 502
BauI CACGAG 1 cut(s) 779
BbsI GAAGAC 1 cut(s) 747
Bbv12I GWGCWC 1 cut(s) 439
BbvI GCAGC 3 cut(s) 56, 87, 343
BceAI ACGGC 1 cut(s) 977
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 4 cut(s) 41, 243, 545, 761
BfoI RGCGCY 1 cut(s) 258
BisI GCNGC 3 cut(s) 45, 101, 332
BlsI GCNGC 3 cut(s) 46, 102, 333
Bme18I GGWCC 2 cut(s) 19, 502
BmeT110I CYCGRG 1 cut(s) 188
BmgT120I GGNCC 2 cut(s) 19, 502
BmiI GGNNCC 1 cut(s) 21
BmrI ACTGGG 1 cut(s) 512
BmsI GCATC 5 cut(s) 121, 189, 453, 876, 935
BmuI ACTGGG 1 cut(s) 512
BpiI GAAGAC 1 cut(s) 747
Bsa29I ATCGAT 1 cut(s) 989
BsaAI YACGTR 1 cut(s) 869
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 2 cut(s) 176, 709
BsaXI ACNNNNNCTCC 2 cut(s) 329, 359
Bsc4I CCNNNNNNNGG 1 cut(s) 658
Bse1I ACTGG 1 cut(s) 518
BseCI ATCGAT 1 cut(s) 989
BseDI CCNNGG 2 cut(s) 176, 709
BseGI GGATG 3 cut(s) 587, 696, 950
BseLI CCNNNNNNNGG 1 cut(s) 658
BseNI ACTGG 1 cut(s) 518
BseRI GAGGAG 3 cut(s) 290, 293, 337
BseXI GCAGC 3 cut(s) 56, 87, 343
BseYI CCCAGC 1 cut(s) 575
Bsh1236I CGCG 1 cut(s) 276
BshVI ATCGAT 1 cut(s) 989
BsiHKAI GWGCWC 1 cut(s) 439
BsiHKCI CYCGRG 1 cut(s) 188
BslFI GGGAC 3 cut(s) 5, 749, 854
BslI CCNNNNNNNGG 1 cut(s) 658
BsmAI GTCTC 1 cut(s) 64
BsmFI GGGAC 3 cut(s) 5, 749, 854
Bso31I GGTCTC 1 cut(s) 64
BsoBI CYCGRG 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 2 cut(s) 151, 990
BspACI CCGC 5 cut(s) 98, 185, 274, 505, 942
BspDI ATCGAT 1 cut(s) 989
BspFNI CGCG 1 cut(s) 276
BspLI GGNNCC 1 cut(s) 21
BspTNI GGTCTC 1 cut(s) 64
BsrI ACTGG 1 cut(s) 518
BssECI CCNNGG 2 cut(s) 176, 709
BssMI GATC 2 cut(s) 151, 990
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 2 cut(s) 176, 709
Bst2BI CACGAG 1 cut(s) 779
Bst4CI ACNGT 2 cut(s) 263, 657
Bst6I CTCTTC 1 cut(s) 347
BstAPI GCANNNNNTGC 2 cut(s) 131, 210
BstBAI YACGTR 1 cut(s) 869
BstF5I GGATG 3 cut(s) 587, 696, 950
BstFNI CGCG 1 cut(s) 276
BstH2I RGCGCY 1 cut(s) 258
BstHHI GCGC 2 cut(s) 204, 257
BstKTI GATC 2 cut(s) 154, 993
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 2 cut(s) 151, 990
BstMWI GCNNNNNNNGC 3 cut(s) 131, 210, 450
BstNSI RCATGY 1 cut(s) 423
BstUI CGCG 1 cut(s) 276
BstV1I GCAGC 3 cut(s) 56, 87, 343
BstV2I GAAGAC 1 cut(s) 747
BstXI CCANNNNNNTGG 1 cut(s) 499
Bsu15I ATCGAT 1 cut(s) 989
BsuTUI ATCGAT 1 cut(s) 989
BtsCI GGATG 3 cut(s) 587, 696, 950
CfoI GCGC 2 cut(s) 204, 257
Cfr13I GGNCC 2 cut(s) 19, 502
ClaI ATCGAT 1 cut(s) 989
Csp6I GTAC 1 cut(s) 866
CviAII CATG 7 cut(s) 128, 420, 481, 499, 723, 823, 844
CviJI RGCY 7 cut(s) 181, 453, 485, 579, 928, 953, 964
CviKI_1 RGCY 7 cut(s) 181, 453, 485, 579, 928, 953, 964
CviQI GTAC 1 cut(s) 866
DpnI GATC 2 cut(s) 153, 992
DpnII GATC 2 cut(s) 151, 990
Eam1104I CTCTTC 1 cut(s) 347
EarI CTCTTC 1 cut(s) 347
EciI GGCGGA 1 cut(s) 957
Eco130I CCWWGG 2 cut(s) 176, 709
Eco31I GGTCTC 1 cut(s) 64
Eco32I GATATC 2 cut(s) 169, 427
Eco47I GGWCC 2 cut(s) 19, 502
Eco88I CYCGRG 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 19
EcoRV GATATC 2 cut(s) 169, 427
EcoT14I CCWWGG 2 cut(s) 176, 709
ErhI CCWWGG 2 cut(s) 176, 709
FaeI CATG 7 cut(s) 131, 423, 484, 502, 726, 826, 847
FaqI GGGAC 3 cut(s) 5, 749, 854
FatI CATG 7 cut(s) 127, 419, 480, 498, 722, 822, 843
FauNDI CATATG 1 cut(s) 158
FblI GTMKAC 1 cut(s) 224
Fnu4HI GCNGC 3 cut(s) 45, 101, 332
FokI GGATG 3 cut(s) 574, 703, 957
Fsp4HI GCNGC 3 cut(s) 45, 101, 332
FspAI RTGCGCAY 1 cut(s) 203
FspBI CTAG 4 cut(s) 41, 243, 545, 761
FspI TGCGCA 1 cut(s) 203
GlaI GCGC 2 cut(s) 203, 256
GluI GCNGC 3 cut(s) 45, 101, 332
GsaI CCCAGC 1 cut(s) 579
HaeII RGCGCY 1 cut(s) 258
HhaI GCGC 2 cut(s) 204, 257
Hin1II CATG 7 cut(s) 131, 423, 484, 502, 726, 826, 847
Hin6I GCGC 2 cut(s) 202, 255
HinP1I GCGC 2 cut(s) 202, 255
HincII GTYRAC 1 cut(s) 834
HindII GTYRAC 1 cut(s) 834
HinfI GANTC 5 cut(s) 137, 191, 532, 640, 773
HphI GGTGA 4 cut(s) 287, 386, 458, 564
Hpy166II GTNNAC 2 cut(s) 225, 834
Hpy188I TCNGA 4 cut(s) 352, 699, 881, 924
Hpy188III TCNNGA 4 cut(s) 32, 362, 779, 968
Hpy8I GTNNAC 2 cut(s) 225, 834
HpyAV CCTTC 1 cut(s) 81
HpyCH4III ACNGT 2 cut(s) 263, 657
HpyCH4IV ACGT 1 cut(s) 868
HpyCH4V TGCA 6 cut(s) 4, 14, 134, 213, 552, 626
HpyF10VI GCNNNNNNNGC 3 cut(s) 131, 210, 450
HpySE526I ACGT 1 cut(s) 868
Hsp92II CATG 7 cut(s) 131, 423, 484, 502, 726, 826, 847
HspAI GCGC 2 cut(s) 202, 255
Kzo9I GATC 2 cut(s) 151, 990
LmnI GCTCC 1 cut(s) 434
LpnPI CCDG 4 cut(s) 17, 531, 589, 658
Lsp1109I GCAGC 3 cut(s) 56, 87, 343
LweI GCATC 5 cut(s) 121, 189, 453, 876, 935
MaeI CTAG 4 cut(s) 41, 243, 545, 761
MaeII ACGT 1 cut(s) 868
MaeIII GTNAC 2 cut(s) 263, 595
MalI GATC 2 cut(s) 153, 992
MboI GATC 2 cut(s) 151, 990
MboII GAAGA 3 cut(s) 20, 334, 752
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 4 cut(s) 475, 732, 860, 875
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 4 cut(s) 401, 639, 668, 676
MroXI GAANNNNTTC 1 cut(s) 718
MseI TTAA 2 cut(s) 401, 753
MslI CAYNNNNRTG 1 cut(s) 848
MspA1I CMGCKG 1 cut(s) 100
MvnI CGCG 1 cut(s) 276
MwoI GCNNNNNNNGC 3 cut(s) 131, 210, 450
NdeI CATATG 1 cut(s) 158
NdeII GATC 2 cut(s) 151, 990
NlaIII CATG 7 cut(s) 131, 423, 484, 502, 726, 826, 847
NlaIV GGNNCC 1 cut(s) 21
NsbI TGCGCA 1 cut(s) 203
NspI RCATGY 1 cut(s) 423
PciI ACATGT 1 cut(s) 419
PdmI GAANNNNTTC 1 cut(s) 718
PfeI GAWTC 4 cut(s) 137, 532, 640, 773
PflMI CCANNNNNTGG 1 cut(s) 658
PkrI GCNGC 3 cut(s) 46, 102, 333
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 869
PpuMI RGGWCCY 1 cut(s) 19
PscI ACATGT 1 cut(s) 419
Psp5II RGGWCCY 1 cut(s) 19
PspFI CCCAGC 1 cut(s) 575
PspN4I GGNNCC 1 cut(s) 21
PspPI GGNCC 2 cut(s) 19, 502
PspPPI RGGWCCY 1 cut(s) 19
RsaI GTAC 1 cut(s) 867
RsaNI GTAC 1 cut(s) 866
RseI CAYNNNNRTG 1 cut(s) 848
SaqAI TTAA 2 cut(s) 401, 753
SatI GCNGC 3 cut(s) 45, 101, 332
Sau3AI GATC 2 cut(s) 151, 990
Sau96I GGNCC 2 cut(s) 19, 502
SchI GAGTC 1 cut(s) 200
SduI GDGCHC 1 cut(s) 439
SfaNI GCATC 5 cut(s) 121, 189, 453, 876, 935
SinI GGWCC 2 cut(s) 19, 502
SmiMI CAYNNNNRTG 1 cut(s) 848
Sse9I AATT 4 cut(s) 475, 732, 860, 875
SsiI CCGC 5 cut(s) 98, 185, 274, 505, 942
SspI AATATT 1 cut(s) 528
SspMI CTAG 4 cut(s) 41, 243, 545, 761
StyI CCWWGG 2 cut(s) 176, 709
TaaI ACNGT 2 cut(s) 263, 657
TaiI ACGT 1 cut(s) 871
TaqI TCGA 1 cut(s) 989
TasI AATT 4 cut(s) 475, 732, 860, 875
TfiI GAWTC 4 cut(s) 137, 532, 640, 773
Tru1I TTAA 2 cut(s) 401, 753
Tru9I TTAA 2 cut(s) 401, 753
TseI GCWGC 3 cut(s) 44, 100, 331
TspDTI ATGAA 1 cut(s) 711
TspGWI ACGGA 1 cut(s) 782
Van91I CCANNNNNTGG 1 cut(s) 658
VpaK11BI GGWCC 2 cut(s) 19, 502
XapI RAATTY 2 cut(s) 860, 875
XceI RCATGY 1 cut(s) 423
XmiI GTMKAC 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 718
XspI CTAG 4 cut(s) 41, 243, 545, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.