Rh4DG085500

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000289
Physical Location & Seq
Forward (+)
13029462 .. 13053530
24069 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG085500.1

Sequence Viewer

Length: 939 bp
ATGGAGGAAGCGGACTCGGACCTCGAACTCTCCGATTCAGAGGAGATTTGCCCGTTAATTTCTCGTTTATTTGAACACGACTCACTGTTCCGGCACCAGATACAGTCATATAATGATTTGATTAGAAAATCCATCCAGAAAATACTCGATGAGGCTAACGACATAGTGGTTGATTGCGGTCCTCAGACCGCGGAGGTTGTTGATGATGAAAGAAGGAAATTAACCTCTGCTCCTCTATCAACCAAGACTGATGAAAGAAATCAAGCTGCATGCATTTCTCAAAATAAGTATTTTGCTACTTCACGCGTGAATCTCACGGTCTATGTTGCACACCACTTTGGATTCAGCTCTCTTACAAACGAGTACAAGGTTCTCCAAATCCAAAGATATAAGCGGGGTTTAATTTTTAACACTTTTGTCCTTGGTACAAGTACTACATGGAGGCAAGTAGAGGTAGACCTTGCTGATCTCCCTTTTGATCCTCTCGAGTTCAAATTTGATACTAGCAGTGTATCCACCTATGGGGCTATACATTGGCTGCACCCAACATTGAATATTATTGTGGCTTTCGACATGAATGGGGCTGAGAGATTTAAGGTGGTGCCAATTCCTGATGGTATTGATCTACATGATGCCCTCGTCAGACTAGATAAAGTGGATGGATGTCTGGCTCTAATAGATGGGCGGAAGCAGCAAAGCTACATGAAGGGGTTATGGATTTTGAAGGATTACAAAAATCATGTCTGGGTTAAGGAGGATATATTGTTTCCTTTTCAGAATGCTATAGTACGGGGCTTAGCCGATCTTCGTTTGCATCAATACAATATGAAGACTAAAAGTTACAGGTTAAGTCAAATTTTGTTGCCGGAATGGAGGAATGATAGTCGAATGTTGGACATGCTGACTAGTTATGATGAAGGCATTGTACCTTTAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box FBA_3

Protein Analysis

312

Amino Acids

36.03

Weight (kDa)

5.51

Isoelectric Point (pI)

35.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FBA_3 PF08268 110 - 264 1.4e-14 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000289)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g12771 FvH4_1g24070 FvH4_1g24080 FvH4_1g24081 FvH4_3g05670 FvH4_3g33611 FvH4_3g43191 FvH4_4g07091 FvH4_4g07100 FvH4_4g28510 FvH4_4g28520 FvH4_4g30181 FvH4_4g30190 FvH4_4g34271 FvH4_4g34340 FvH4_5g31290 FvH4_6g37661 FvH4_7g33810
malus_domestica MD02G1067600.v1.1 MD03G1110700.v1.1 MD11G1124100.v1.1 MD11G1124200.v1.1 MD11G1124500.v1.1 MD13G1020600.v1.1 MD16G1019000.v1.1
prunus_persica Prupe.1G283900_v2.0.a1 Prupe.1G284000_v2.0.a1 Prupe.1G285200_v2.0.a1 Prupe.1G285300_v2.0.a1 Prupe.1G285500_v2.0.a1 Prupe.1G285600_v2.0.a1 Prupe.1G286300_v2.0.a1 Prupe.1G300600_v2.0.a1 Prupe.1G300900_v2.0.a1 Prupe.1G333100_v2.0.a1 Prupe.6G093100_v2.0.a1
pyrus_communis pycom02g05360 pycom04g05300 pycom11g10460 pycom11g10470 pycom11g10510 pycom13g01740 pycom13g01750 pycom13g04550
rosa_chinensis RchiOBHm_Chr2g0138411 RchiOBHm_Chr2g0138421 RchiOBHm_Chr4g0399251 RchiOBHm_Chr4g0436521
rosa_laevigata RLG00000005896 RLG00000005933 RLG00000006379 RLG00000006511 RLG00000009284 RLG00000019741 RLG00000019742 RLG00000019743 RLG00000020150 RLG00000024158 RLG00000024159 RLG00000030139 RLG00000032057
rosa_multiflora Rmu_co8310765.1_g000001 Rmu_co8476365.1_g000001 Rmu_sc0001426.1_g000004 Rmu_sc0001534.1_g000001 Rmu_sc0001534.1_g000009 Rmu_sc0001534.1_g000010 Rmu_sc0001556.1_g000019 Rmu_sc0001556.1_g000020 Rmu_sc0001779.1_g000020 Rmu_sc0003130.1_g000005 Rmu_sc0005961.1_g000007 Rmu_sc0025546.1_g000001 Rmu_sc0035131.1_g000001 Rmu_sc0035132.1_g000001
rosa_roxburghii Rroxscaffold_1G00062740 Rroxscaffold_2G00106090 Rroxscaffold_2G00106130 Rroxscaffold_2G00106210 Rroxscaffold_4G00324740 Rroxscaffold_4G00324760
rosa_rugosa Rorug04G0015700 Rorug04G0015800 Rorug04G0294500 Rorug04G0345900 Rorug04G0345900 Rorug05G0013800 Rorug05G0336100 Rorug05G0446400
rosa_samantha Rh2AG399300 Rh2BG407000 Rh2BG407300 Rh2BG407400 Rh2CG385800 Rh2DG419200 Rh4AG093400 Rh4AG348800 Rh4AG401200 Rh4AG406800 Rh4AG406900 Rh4CG101600 Rh4CG371900 Rh4CG428200 Rh4CG428300 Rh4DG085500 Rh4DG085700 Rh4DG351400 Rh4DG365000 Rh4DG408300 Rh4DG408400 Rh5AG108000 Rh5BG104700 Rh5BG411400 Rh5BG523400 Rh5DG103300 Rh5DG425800
rosa_wichuraiana Rw0G008940 Rw1G005620 Rw3G022740 Rw4G030510 Rw4G034940 Rw5G009440 Rw5G037570 Rw5G046580

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 93, 601
AccI GTMKAC 1 cut(s) 456
AccII CGCG 2 cut(s) 191, 306
AciI CCGC 6 cut(s) 11, 177, 189, 191, 394, 685
AclWI GGATC 1 cut(s) 473
AcsI RAATTY 2 cut(s) 494, 855
AfaI GTAC 5 cut(s) 365, 427, 433, 789, 927
AfiI CCNNNNNNNGG 1 cut(s) 522
AflIII ACRYGT 1 cut(s) 304
AgsI TTSAA 4 cut(s) 74, 493, 553, 724
AhlI ACTAGT 1 cut(s) 905
AluBI AGCT 3 cut(s) 266, 348, 699
AluI AGCT 3 cut(s) 266, 348, 699
AlwI GGATC 1 cut(s) 473
Ama87I CYCGRG 1 cut(s) 485
ApeKI GCWGC 3 cut(s) 266, 538, 691
ApoI RAATTY 2 cut(s) 494, 855
ArsI GACNNNNNNTTYG 2 cut(s) 726, 758
AspS9I GGNCC 2 cut(s) 19, 179
AvaI CYCGRG 1 cut(s) 485
AvaII GGWCC 2 cut(s) 19, 179
BanI GGYRCC 2 cut(s) 93, 601
BarI GAAGNNNNNNTAC 1 cut(s) 909
BbsI GAAGAC 1 cut(s) 836
BbvI GCAGC 3 cut(s) 253, 525, 703
BccI CCATC 4 cut(s) 140, 608, 653, 674
BciVI GTATCC 1 cut(s) 523
BcuI ACTAGT 1 cut(s) 905
BfaI CTAG 3 cut(s) 504, 647, 906
BfmI CTRYAG 1 cut(s) 783
BfuI GTATCC 1 cut(s) 523
BisI GCNGC 3 cut(s) 267, 539, 692
BlpI GCTNAGC 1 cut(s) 796
BlsI GCNGC 3 cut(s) 268, 540, 693
BmcAI AGTACT 1 cut(s) 433
Bme18I GGWCC 2 cut(s) 19, 179
BmeT110I CYCGRG 1 cut(s) 485
BmgT120I GGNCC 2 cut(s) 19, 179
BmiI GGNNCC 2 cut(s) 95, 603
BmsI GCATC 2 cut(s) 622, 823
BpiI GAAGAC 1 cut(s) 836
Bpu1102I GCTNAGC 1 cut(s) 796
BsaJI CCNNGG 2 cut(s) 189, 421
BsaXI ACNNNNNCTCC 2 cut(s) 214, 244
Bsc4I CCNNNNNNNGG 1 cut(s) 522
BseDI CCNNGG 2 cut(s) 189, 421
BseGI GGATG 3 cut(s) 132, 664, 668
BseLI CCNNNNNNNGG 1 cut(s) 522
BseMII CTCAG 2 cut(s) 197, 576
BseRI GAGGAG 2 cut(s) 56, 222
BseXI GCAGC 3 cut(s) 253, 525, 703
BsgI GTGCAG 1 cut(s) 524
Bsh1236I CGCG 2 cut(s) 191, 306
BshNI GGYRCC 2 cut(s) 93, 601
BsiHKCI CYCGRG 1 cut(s) 485
BsiSI CCGG 2 cut(s) 91, 866
BslI CCNNNNNNNGG 1 cut(s) 522
BsmI GAATGC 1 cut(s) 784
BsoBI CYCGRG 1 cut(s) 485
Bsp143I GATC 4 cut(s) 466, 478, 622, 802
Bsp1720I GCTNAGC 1 cut(s) 796
BspACI CCGC 6 cut(s) 11, 177, 189, 191, 394, 685
BspCNI CTCAG 2 cut(s) 196, 577
BspFNI CGCG 2 cut(s) 191, 306
BspLI GGNNCC 2 cut(s) 95, 603
BspPI GGATC 1 cut(s) 473
BspT107I GGYRCC 2 cut(s) 93, 601
BssECI CCNNGG 2 cut(s) 189, 421
BssMI GATC 4 cut(s) 466, 478, 622, 802
BssT1I CCWWGG 1 cut(s) 421
Bst4CI ACNGT 3 cut(s) 87, 105, 319
BstC8I GCNNGC 1 cut(s) 271
BstDEI CTNAG 3 cut(s) 183, 585, 796
BstDSI CCRYGG 1 cut(s) 189
BstF5I GGATG 3 cut(s) 132, 664, 668
BstFNI CGCG 2 cut(s) 191, 306
BstKTI GATC 4 cut(s) 469, 481, 625, 805
BstMBI GATC 4 cut(s) 466, 478, 622, 802
BstMWI GCNNNNNNNGC 1 cut(s) 691
BstNSI RCATGY 2 cut(s) 273, 901
BstSFI CTRYAG 1 cut(s) 783
BstUI CGCG 2 cut(s) 191, 306
BstV1I GCAGC 3 cut(s) 253, 525, 703
BstV2I GAAGAC 1 cut(s) 836
BsuI GTATCC 1 cut(s) 523
BtgI CCRYGG 1 cut(s) 189
BtsCI GGATG 3 cut(s) 132, 664, 668
BtsI GCAGTG 1 cut(s) 514
BtsIMutI CAGTG 2 cut(s) 83, 514
Cac8I GCNNGC 1 cut(s) 271
Cfr13I GGNCC 2 cut(s) 19, 179
Cfr42I CCGCGG 1 cut(s) 192
Csp6I GTAC 5 cut(s) 364, 426, 432, 788, 926
CviAII CATG 7 cut(s) 270, 438, 574, 629, 703, 740, 898
CviQI GTAC 5 cut(s) 364, 426, 432, 788, 926
DdeI CTNAG 3 cut(s) 183, 585, 796
DpnI GATC 4 cut(s) 468, 480, 624, 804
DpnII GATC 4 cut(s) 466, 478, 622, 802
EciI GGCGGA 1 cut(s) 700
Eco130I CCWWGG 1 cut(s) 421
Eco47I GGWCC 2 cut(s) 19, 179
Eco88I CYCGRG 1 cut(s) 485
EcoT14I CCWWGG 1 cut(s) 421
EcoT22I ATGCAT 1 cut(s) 275
ErhI CCWWGG 1 cut(s) 421
FaeI CATG 7 cut(s) 273, 441, 577, 632, 706, 743, 901
FatI CATG 7 cut(s) 269, 437, 573, 628, 702, 739, 897
FauI CCCGC 1 cut(s) 387
FblI GTMKAC 1 cut(s) 456
Fnu4HI GCNGC 3 cut(s) 267, 539, 692
FokI GGATG 3 cut(s) 119, 671, 675
Fsp4HI GCNGC 3 cut(s) 267, 539, 692
FspBI CTAG 3 cut(s) 504, 647, 906
GluI GCNGC 3 cut(s) 267, 539, 692
HapII CCGG 2 cut(s) 91, 866
Hin1II CATG 7 cut(s) 273, 441, 577, 632, 706, 743, 901
HinfI GANTC 5 cut(s) 14, 35, 80, 310, 342
HpaII CCGG 2 cut(s) 91, 866
Hpy166II GTNNAC 1 cut(s) 457
Hpy188I TCNGA 6 cut(s) 19, 34, 40, 186, 644, 777
Hpy188III TCNNGA 3 cut(s) 136, 485, 611
Hpy8I GTNNAC 1 cut(s) 457
HpyAV CCTTC 4 cut(s) 207, 700, 718, 911
HpyCH4III ACNGT 3 cut(s) 87, 105, 319
HpyCH4V TGCA 5 cut(s) 269, 273, 329, 541, 814
HpyF10VI GCNNNNNNNGC 1 cut(s) 691
HpyF3I CTNAG 3 cut(s) 183, 585, 796
Hsp92II CATG 7 cut(s) 273, 441, 577, 632, 706, 743, 901
KspI CCGCGG 1 cut(s) 192
Kzo9I GATC 4 cut(s) 466, 478, 622, 802
LmnI GCTCC 1 cut(s) 235
LpnPI CCDG 8 cut(s) 104, 110, 149, 624, 653, 730, 829, 879
Lsp1109I GCAGC 3 cut(s) 253, 525, 703
LweI GCATC 2 cut(s) 622, 823
MaeI CTAG 3 cut(s) 504, 647, 906
MaeIII GTNAC 1 cut(s) 839
MalI GATC 4 cut(s) 468, 480, 624, 804
MboI GATC 4 cut(s) 466, 478, 622, 802
MboII GAAGA 2 cut(s) 797, 841
MluCI AATT 6 cut(s) 57, 218, 402, 494, 606, 855
MluI ACGCGT 1 cut(s) 304
MlyI GAGTC 2 cut(s) 8, 74
MmeI TCCRAC 1 cut(s) 873
Mph1103I ATGCAT 1 cut(s) 275
MseI TTAA 8 cut(s) 56, 221, 401, 408, 594, 750, 848, 932
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 2 cut(s) 91, 866
Mva1269I GAATGC 1 cut(s) 784
MvnI CGCG 2 cut(s) 191, 306
MwoI GCNNNNNNNGC 1 cut(s) 691
NdeII GATC 4 cut(s) 466, 478, 622, 802
NlaIII CATG 7 cut(s) 273, 441, 577, 632, 706, 743, 901
NlaIV GGNNCC 2 cut(s) 95, 603
NsiI ATGCAT 1 cut(s) 275
NspI RCATGY 2 cut(s) 273, 901
PaeI GCATGC 1 cut(s) 273
PaeR7I CTCGAG 1 cut(s) 485
PcsI WCGNNNNNNNCGW 1 cut(s) 30
PctI GAATGC 1 cut(s) 784
PfeI GAWTC 3 cut(s) 35, 310, 342
PkrI GCNGC 3 cut(s) 268, 540, 693
PleI GAGTC 2 cut(s) 8, 74
PpsI GAGTC 2 cut(s) 8, 74
PspN4I GGNNCC 2 cut(s) 95, 603
PspPI GGNCC 2 cut(s) 19, 179
RsaI GTAC 5 cut(s) 365, 427, 433, 789, 927
RsaNI GTAC 5 cut(s) 364, 426, 432, 788, 926
SacII CCGCGG 1 cut(s) 192
SaqAI TTAA 8 cut(s) 56, 221, 401, 408, 594, 750, 848, 932
SatI GCNGC 3 cut(s) 267, 539, 692
Sau3AI GATC 4 cut(s) 466, 478, 622, 802
Sau96I GGNCC 2 cut(s) 19, 179
ScaI AGTACT 1 cut(s) 433
SchI GAGTC 2 cut(s) 8, 74
SfaNI GCATC 2 cut(s) 622, 823
SfcI CTRYAG 1 cut(s) 783
Sfr274I CTCGAG 1 cut(s) 485
Sfr303I CCGCGG 1 cut(s) 192
SgrBI CCGCGG 1 cut(s) 192
SinI GGWCC 2 cut(s) 19, 179
SlaI CTCGAG 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
SpeI ACTAGT 1 cut(s) 905
SphI GCATGC 1 cut(s) 273
Sse9I AATT 6 cut(s) 57, 218, 402, 494, 606, 855
SsiI CCGC 6 cut(s) 11, 177, 189, 191, 394, 685
SspI AATATT 1 cut(s) 556
SspMI CTAG 3 cut(s) 504, 647, 906
StyI CCWWGG 1 cut(s) 421
TaaI ACNGT 3 cut(s) 87, 105, 319
TaqI TCGA 5 cut(s) 24, 147, 486, 570, 886
TasI AATT 6 cut(s) 57, 218, 402, 494, 606, 855
TatI WGTACW 2 cut(s) 363, 431
TfiI GAWTC 3 cut(s) 35, 310, 342
Tru1I TTAA 8 cut(s) 56, 221, 401, 408, 594, 750, 848, 932
Tru9I TTAA 8 cut(s) 56, 221, 401, 408, 594, 750, 848, 932
TscAI CASTG 2 cut(s) 90, 514
TseI GCWGC 3 cut(s) 266, 538, 691
TspDTI ATGAA 6 cut(s) 222, 267, 590, 719, 842, 930
TspRI CASTG 2 cut(s) 90, 514
VpaK11BI GGWCC 2 cut(s) 19, 179
XapI RAATTY 2 cut(s) 494, 855
XceI RCATGY 2 cut(s) 273, 901
XhoI CTCGAG 1 cut(s) 485
XmiI GTMKAC 1 cut(s) 456
XspI CTAG 3 cut(s) 504, 647, 906
ZrmI AGTACT 1 cut(s) 433
Zsp2I ATGCAT 1 cut(s) 275
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.