MD10G1334300.v1.1

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000236
Physical Location & Seq
Forward (+)
41149308 .. 41152887
3580 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1334300.v1.1.491

Sequence Viewer

Length: 1686 bp
ATGGATGAAGGCAGGCGGAAGAACACAGAAACAAAGAGGAAGAAAAAGGAAAAAATAGGTGACGAGCATCCAGACACTTTAAAAAAATTGAGTATGTTCGGATTGAGCTATGGATTTCCGTTGGGTATGTCAAGCGAAGAGCCCCCTAGTCAAGAAGAAATAGGCGATGAGCATCAAGACAGCCAAGAGCTCCCCAGTCAAGAAGAAATAGGTGATGAGCATCAAGACAGCAAAGAGCTCCTTAGTCAAGAAGAAATAGGCGTTGAGCATCAAGACAACGAAAAGCTCCCCAGTCAAGAAGAAATAGGTGATGAGCAGAGAAACACAATGGATGAAGGCAGGCGGAAGAACACAGAAACAAAGAGGAAGAAAAAGGAAAAAATAGGTGATGAGCATCCATACACTTTCAAAAAGTTGGGTATGTTCGGATTGAGCTATGGATTTCCGTTGGGTATGTCAAGCGAAGAGCTCCTTAGTCAAGATGAAATAGGCGATGAGCATCAAGACAGCAAAGAGCTCCCCAGTCAAGAAGAAATAAGTGATGAGCAGAGAAACACGAAAGGTCAAGAAGAAATAGGTGATGAGAGAAACACGAAAGATCAAGGCAGGCGGAAGAACATAGAAACAAGGAGGAAGAAAAAGGAAAAAATAGGTGATGAGCATCCAGACAACGTCAAAGAATTAATAAATGCTACCGAAAATTTTAGCGACAAAAAAAGACTTGGCCATTCTGAGACATTTTTTATGGCACAACTGCCAAGTCATACCGTGGCCGTGAAGAAACTAGATTCCGCTCATTTTGAGGGAAAAATCGATAAACTGAAAGAGGAAATTGGCATCATAGAGTCATTGCAACACAACAATATCCTTAAACTGTTGCATTCTTATATTGGAAAAGACCTCCAATTTCTTGTTTACGAATACATGGAAAATAAATCCCTTGAAGACATCTTATTTGGCTCGAGTACTTCTGGCACAATCAAGCTTGATTGGAATACAAGGGTTAACATTTGCTTGGGAATAGCACAGGGTTTGCAATATCTACATGAGAGAGTACAGATTGTTCATACGAATATAAAATCCGCTAATATTCTTCTTAATGAAAAACTTGAGGCTAAGATATCGGACTTTGGATTTGCAAATCTTTATTCCGAAGAAGATAAAGTTATGGCCATCGCAAGAGAAACAAAGAAAGGCTACACGGCGCCAGAGTATTTGCAAATGGATGATTTAGATAGCAAACTGGATGTTTTCAGCTTTGGGGTGGTCGTACTTGAAATTGTTAGTGGGGAGAGAAACGTACGTAACCAATCAAAGAAGGAAACTGAGGTTCTTTTAGACAGGGCTTATAAAGCAAATAGAAACGGAAATTTGAAGAGCTTGGTTGATAAGAATTTGTCTACATATGATGAAAGAGAAGCCCTCATCATCTTGAAATTAGCATTGGAGTGCACCACGATGGGTGCTAGTGTCAGACCTGAAATGTCTGGAGTTGTGAGTGTTCTTCTTGGCGAAAAAAGCATTGACGAGGTTTGTTCACCTGCCAAGCCCACTGGCGACATCAATGTTGTTGGTTCCCTCGAAGATCGGCAGGCATTTCTGATATGGCAGAGTCTCTTTCCCCACTTTGGGGAAGTTGACTTCAGTAGCATCAACTTCGTCTCATTCAACCTAAGGCGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

562

Amino Acids

63.91

Weight (kDa)

5.16

Isoelectric Point (pI)

53.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 254 - 448 3e-37 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 254 - 459 6.2e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1350
AarI CACCTGC 1 cut(s) 1549
Acc36I ACCTGC 1 cut(s) 1549
AccB1I GGYRCC 1 cut(s) 1204
AccBSI CCGCTC 1 cut(s) 794
AccI GTMKAC 1 cut(s) 1400
AciI CCGC 5 cut(s) 16, 343, 610, 792, 1083
AcoI YGGCCR 3 cut(s) 724, 771, 1170
AcsI RAATTY 3 cut(s) 700, 1369, 1393
AcuI CTGAAG 1 cut(s) 1627
AcyI GRCGYC 1 cut(s) 1205
AfaI GTAC 4 cut(s) 967, 1056, 1272, 1302
AfiI CCNNNNNNNGG 3 cut(s) 1628, 1629, 1630
AgsI TTSAA 6 cut(s) 409, 944, 1277, 1375, 1435, 1669
AjuI GAANNNNNNNTTGG 2 cut(s) 1427, 1459
Alw21I GWGCWC 5 cut(s) 192, 240, 471, 519, 1454
Alw26I GTCTC 3 cut(s) 728, 1619, 1666
Alw44I GTGCAC 1 cut(s) 1450
Ama87I CYCGRG 1 cut(s) 961
AoxI GGCC 3 cut(s) 724, 771, 1170
ApaLI GTGCAC 1 cut(s) 1450
ApoI RAATTY 3 cut(s) 700, 1369, 1393
ArsI GACNNNNNNTTYG 4 cut(s) 938, 970, 1118, 1150
AseI ATTAAT 1 cut(s) 683
AspLEI GCGC 2 cut(s) 1207, 1680
AsuHPI GGTGA 7 cut(s) 71, 224, 320, 398, 590, 665, 1530
AvaI CYCGRG 1 cut(s) 961
AxyI CCTNAGG 1 cut(s) 1673
BaeGI GKGCMC 1 cut(s) 1454
BalI TGGCCA 2 cut(s) 726, 1172
BanI GGYRCC 1 cut(s) 1204
BanII GRGCYC 5 cut(s) 144, 192, 240, 471, 519
BbsI GAAGAC 1 cut(s) 951
Bbv12I GWGCWC 5 cut(s) 192, 240, 471, 519, 1454
BccI CCATC 2 cut(s) 1181, 1453
BceAI ACGGC 2 cut(s) 758, 1218
BcgI CGANNNNNNTGC 2 cut(s) 1639, 1673
BcoDI GTCTC 3 cut(s) 728, 1619, 1666
BfaI CTAG 3 cut(s) 147, 785, 1467
BfoI RGCGCY 1 cut(s) 1208
BfuAI ACCTGC 1 cut(s) 1549
BmcAI AGTACT 1 cut(s) 967
BmeT110I CYCGRG 1 cut(s) 961
BmiI GGNNCC 2 cut(s) 1206, 1576
BmrI ACTGGG 3 cut(s) 189, 285, 516
BmsI GCATC 9 cut(s) 76, 181, 229, 277, 403, 508, 670, 846, 1659
BmuI ACTGGG 3 cut(s) 189, 285, 516
BpiI GAAGAC 1 cut(s) 951
BplI GAGNNNNNCTC 2 cut(s) 1407, 1439
BpmI CTGGAG 1 cut(s) 1509
BpuEI CTTGAG 1 cut(s) 1130
Bsa29I ATCGAT 1 cut(s) 813
BsaAI YACGTR 1 cut(s) 1304
BsaBI GATNNNNATC 5 cut(s) 171, 219, 393, 498, 660
BsaHI GRCGYC 1 cut(s) 1205
BsaJI CCNNGG 1 cut(s) 768
Bsc4I CCNNNNNNNGG 3 cut(s) 1628, 1629, 1630
Bse1I ACTGG 5 cut(s) 195, 291, 522, 1248, 1558
Bse21I CCTNAGG 1 cut(s) 1673
Bse3DI GCAATG 1 cut(s) 848
Bse8I GATNNNNATC 5 cut(s) 171, 219, 393, 498, 660
BseCI ATCGAT 1 cut(s) 813
BseDI CCNNGG 1 cut(s) 768
BseGI GGATG 7 cut(s) 10, 67, 337, 394, 661, 1231, 1252
BseJI GATNNNNATC 5 cut(s) 171, 219, 393, 498, 660
BseLI CCNNNNNNNGG 3 cut(s) 1628, 1629, 1630
BseMI GCAATG 1 cut(s) 848
BseMII CTCAG 2 cut(s) 723, 1317
BseNI ACTGG 5 cut(s) 195, 291, 522, 1248, 1558
BseSI GKGCMC 1 cut(s) 1454
BshFI GGCC 3 cut(s) 726, 773, 1172
BshNI GGYRCC 1 cut(s) 1204
BshVI ATCGAT 1 cut(s) 813
BsiHKAI GWGCWC 5 cut(s) 192, 240, 471, 519, 1454
BsiHKCI CYCGRG 1 cut(s) 961
BsiWI CGTACG 1 cut(s) 1300
BslI CCNNNNNNNGG 3 cut(s) 1628, 1629, 1630
BsmAI GTCTC 3 cut(s) 728, 1619, 1666
BsmBI CGTCTC 1 cut(s) 1666
BsmI GAATGC 1 cut(s) 880
BsnI GGCC 3 cut(s) 726, 773, 1172
BsoBI CYCGRG 1 cut(s) 961
Bsp1286I GDGCHC 6 cut(s) 144, 192, 240, 471, 519, 1454
Bsp143I GATC 2 cut(s) 598, 1585
BspACI CCGC 5 cut(s) 16, 343, 610, 792, 1083
BspANI GGCC 3 cut(s) 726, 773, 1172
BspCNI CTCAG 2 cut(s) 724, 1318
BspDI ATCGAT 1 cut(s) 813
BspLI GGNNCC 2 cut(s) 1206, 1576
BspMI ACCTGC 1 cut(s) 1549
BspQI GCTCTTC 3 cut(s) 132, 459, 1370
BspT107I GGYRCC 1 cut(s) 1204
BsrBI CCGCTC 1 cut(s) 794
BsrDI GCAATG 1 cut(s) 848
BsrI ACTGG 5 cut(s) 195, 291, 522, 1248, 1558
BssECI CCNNGG 1 cut(s) 768
BssMI GATC 2 cut(s) 598, 1585
BssNI GRCGYC 1 cut(s) 1205
Bst4CI ACNGT 2 cut(s) 769, 876
Bst6I CTCTTC 3 cut(s) 132, 459, 1370
BstACI GRCGYC 1 cut(s) 1205
BstBAI YACGTR 1 cut(s) 1304
BstC8I GCNNGC 4 cut(s) 14, 341, 608, 1593
BstDEI CTNAG 6 cut(s) 242, 473, 732, 1116, 1326, 1673
BstDSI CCRYGG 1 cut(s) 768
BstF5I GGATG 7 cut(s) 10, 67, 337, 394, 661, 1231, 1252
BstH2I RGCGCY 1 cut(s) 1208
BstHHI GCGC 2 cut(s) 1207, 1680
BstKTI GATC 2 cut(s) 601, 1588
BstMAI GTCTC 3 cut(s) 728, 1619, 1666
BstMBI GATC 2 cut(s) 598, 1585
BstMWI GCNNNNNNNGC 2 cut(s) 1352, 1518
BstSLI GKGCMC 1 cut(s) 1454
BstSNI TACGTA 1 cut(s) 1304
BstV2I GAAGAC 1 cut(s) 951
Bsu15I ATCGAT 1 cut(s) 813
Bsu36I CCTNAGG 1 cut(s) 1673
BsuRI GGCC 3 cut(s) 726, 773, 1172
BsuTUI ATCGAT 1 cut(s) 813
BtgI CCRYGG 1 cut(s) 768
BtgZI GCGATG 3 cut(s) 180, 507, 1159
BtsCI GGATG 7 cut(s) 10, 67, 337, 394, 661, 1231, 1252
BtsIMutI CAGTG 1 cut(s) 1551
BveI ACCTGC 1 cut(s) 1549
Cac8I GCNNGC 4 cut(s) 14, 341, 608, 1593
CfoI GCGC 2 cut(s) 1207, 1680
ClaI ATCGAT 1 cut(s) 813
Csp6I GTAC 4 cut(s) 966, 1055, 1271, 1301
CviAII CATG 3 cut(s) 925, 1046, 1683
CviQI GTAC 4 cut(s) 966, 1055, 1271, 1301
DdeI CTNAG 6 cut(s) 242, 473, 732, 1116, 1326, 1673
DinI GGCGCC 1 cut(s) 1206
DpnI GATC 2 cut(s) 600, 1587
DpnII GATC 2 cut(s) 598, 1585
DraI TTTAAA 1 cut(s) 81
EaeI YGGCCR 3 cut(s) 724, 771, 1170
Eam1104I CTCTTC 3 cut(s) 132, 459, 1370
EarI CTCTTC 3 cut(s) 132, 459, 1370
EciI GGCGGA 3 cut(s) 31, 358, 625
Ecl136II GAGCTC 4 cut(s) 190, 238, 469, 517
Eco105I TACGTA 1 cut(s) 1304
Eco24I GRGCYC 5 cut(s) 144, 192, 240, 471, 519
Eco32I GATATC 1 cut(s) 1122
Eco53kI GAGCTC 4 cut(s) 190, 238, 469, 517
Eco57I CTGAAG 1 cut(s) 1627
Eco81I CCTNAGG 1 cut(s) 1673
Eco88I CYCGRG 1 cut(s) 961
EcoICRI GAGCTC 4 cut(s) 190, 238, 469, 517
EcoRV GATATC 1 cut(s) 1122
EcoT38I GRGCYC 5 cut(s) 144, 192, 240, 471, 519
EgeI GGCGCC 1 cut(s) 1206
EheI GGCGCC 1 cut(s) 1206
Esp3I CGTCTC 1 cut(s) 1666
FaeI CATG 3 cut(s) 928, 1049, 1686
FalI AAGNNNNNCTT 4 cut(s) 225, 257, 456, 488
FatI CATG 3 cut(s) 924, 1045, 1682
FauNDI CATATG 1 cut(s) 1405
FblI GTMKAC 1 cut(s) 1400
FokI GGATG 7 cut(s) 17, 54, 344, 381, 648, 1238, 1259
FriOI GRGCYC 5 cut(s) 144, 192, 240, 471, 519
FspBI CTAG 3 cut(s) 147, 785, 1467
GlaI GCGC 2 cut(s) 1206, 1679
GsuI CTGGAG 1 cut(s) 1509
HaeII RGCGCY 1 cut(s) 1208
HaeIII GGCC 3 cut(s) 726, 773, 1172
HhaI GCGC 2 cut(s) 1207, 1680
Hin1I GRCGYC 1 cut(s) 1205
Hin1II CATG 3 cut(s) 928, 1049, 1686
Hin6I GCGC 2 cut(s) 1205, 1678
HinP1I GCGC 2 cut(s) 1205, 1678
HincII GTYRAC 2 cut(s) 1006, 1639
HindII GTYRAC 2 cut(s) 1006, 1639
HindIII AAGCTT 1 cut(s) 983
HinfI GANTC 3 cut(s) 788, 845, 1612
HpaI GTTAAC 1 cut(s) 1006
HphI GGTGA 7 cut(s) 71, 224, 320, 398, 590, 665, 1530
Hpy166II GTNNAC 6 cut(s) 916, 1006, 1401, 1452, 1538, 1639
Hpy188I TCNGA 7 cut(s) 101, 428, 733, 1126, 1153, 1475, 1602
Hpy8I GTNNAC 6 cut(s) 916, 1006, 1401, 1452, 1538, 1639
HpyAV CCTTC 2 cut(s) 329, 1312
HpyCH4III ACNGT 2 cut(s) 769, 876
HpyCH4IV ACGT 3 cut(s) 672, 1299, 1303
HpyCH4V TGCA 6 cut(s) 853, 880, 1036, 1139, 1219, 1452
HpyF10VI GCNNNNNNNGC 2 cut(s) 1352, 1518
HpyF3I CTNAG 6 cut(s) 242, 473, 732, 1116, 1326, 1673
HpySE526I ACGT 3 cut(s) 672, 1299, 1303
Hsp92I GRCGYC 1 cut(s) 1205
Hsp92II CATG 3 cut(s) 928, 1049, 1686
HspAI GCGC 2 cut(s) 1205, 1678
KasI GGCGCC 1 cut(s) 1204
KspAI GTTAAC 1 cut(s) 1006
Kzo9I GATC 2 cut(s) 598, 1585
LguI GCTCTTC 3 cut(s) 132, 459, 1370
LmnI GCTCC 5 cut(s) 195, 243, 291, 474, 522
LweI GCATC 9 cut(s) 76, 181, 229, 277, 403, 508, 670, 846, 1659
MaeI CTAG 3 cut(s) 147, 785, 1467
MaeII ACGT 3 cut(s) 672, 1299, 1303
MaeIII GTNAC 2 cut(s) 59, 1304
MalI GATC 2 cut(s) 600, 1587
MbiI CCGCTC 1 cut(s) 794
MboI GATC 2 cut(s) 598, 1585
MhlI GDGCHC 6 cut(s) 144, 192, 240, 471, 519, 1454
MlsI TGGCCA 2 cut(s) 726, 1172
MluCI AATT 9 cut(s) 86, 680, 700, 831, 905, 1278, 1369, 1393, 1436
MluNI TGGCCA 2 cut(s) 726, 1172
Mly113I GGCGCC 1 cut(s) 1205
MlyI GAGTC 2 cut(s) 854, 1621
Mox20I TGGCCA 2 cut(s) 726, 1172
MscI TGGCCA 2 cut(s) 726, 1172
MseI TTAA 5 cut(s) 80, 683, 870, 1005, 1098
MslI CAYNNNNRTG 2 cut(s) 1447, 1457
Msp20I TGGCCA 2 cut(s) 726, 1172
Mva1269I GAATGC 1 cut(s) 880
MwoI GCNNNNNNNGC 2 cut(s) 1352, 1518
NarI GGCGCC 1 cut(s) 1205
NdeI CATATG 1 cut(s) 1405
NdeII GATC 2 cut(s) 598, 1585
NlaIII CATG 3 cut(s) 928, 1049, 1686
NlaIV GGNNCC 2 cut(s) 1206, 1576
NmuCI GTSAC 1 cut(s) 59
PaeR7I CTCGAG 1 cut(s) 961
PaqCI CACCTGC 1 cut(s) 1549
PciSI GCTCTTC 3 cut(s) 132, 459, 1370
PctI GAATGC 1 cut(s) 880
PfeI GAWTC 1 cut(s) 788
Pfl23II CGTACG 1 cut(s) 1300
PflFI GACNNNGTC 1 cut(s) 671
PleI GAGTC 2 cut(s) 853, 1620
PluTI GGCGCC 1 cut(s) 1208
PpsI GAGTC 2 cut(s) 853, 1620
Ppu21I YACGTR 1 cut(s) 1304
PshBI ATTAAT 1 cut(s) 683
PsiI TTATAA 1 cut(s) 1350
Psp124BI GAGCTC 4 cut(s) 192, 240, 471, 519
PspLI CGTACG 1 cut(s) 1300
PspN4I GGNNCC 2 cut(s) 1206, 1576
PspXI VCTCGAGB 1 cut(s) 961
PsrI GAACNNNNNNTAC 2 cut(s) 1047, 1079
PsyI GACNNNGTC 1 cut(s) 671
RsaI GTAC 4 cut(s) 967, 1056, 1272, 1302
RsaNI GTAC 4 cut(s) 966, 1055, 1271, 1301
RseI CAYNNNNRTG 2 cut(s) 1447, 1457
SacI GAGCTC 4 cut(s) 192, 240, 471, 519
SapI GCTCTTC 3 cut(s) 132, 459, 1370
SaqAI TTAA 5 cut(s) 80, 683, 870, 1005, 1098
Sau3AI GATC 2 cut(s) 598, 1585
ScaI AGTACT 1 cut(s) 967
SchI GAGTC 2 cut(s) 854, 1621
SduI GDGCHC 6 cut(s) 144, 192, 240, 471, 519, 1454
SfaNI GCATC 9 cut(s) 76, 181, 229, 277, 403, 508, 670, 846, 1659
SfoI GGCGCC 1 cut(s) 1206
Sfr274I CTCGAG 1 cut(s) 961
SlaI CTCGAG 1 cut(s) 961
SmiMI CAYNNNNRTG 2 cut(s) 1447, 1457
SmlI CTYRAG 2 cut(s) 961, 1109
SmoI CTYRAG 2 cut(s) 961, 1109
SnaBI TACGTA 1 cut(s) 1304
Sse9I AATT 9 cut(s) 86, 680, 700, 831, 905, 1278, 1369, 1393, 1436
SsiI CCGC 5 cut(s) 16, 343, 610, 792, 1083
SspDI GGCGCC 1 cut(s) 1204
SspI AATATT 1 cut(s) 1090
SspMI CTAG 3 cut(s) 147, 785, 1467
SstI GAGCTC 4 cut(s) 192, 240, 471, 519
TaaI ACNGT 2 cut(s) 769, 876
TaiI ACGT 3 cut(s) 675, 1302, 1306
TaqI TCGA 3 cut(s) 813, 962, 1581
TasI AATT 9 cut(s) 86, 680, 700, 831, 905, 1278, 1369, 1393, 1436
TatI WGTACW 2 cut(s) 965, 1054
TfiI GAWTC 1 cut(s) 788
Tru1I TTAA 5 cut(s) 80, 683, 870, 1005, 1098
Tru9I TTAA 5 cut(s) 80, 683, 870, 1005, 1098
TscAI CASTG 1 cut(s) 1558
TseFI GTSAC 1 cut(s) 59
Tsp45I GTSAC 1 cut(s) 59
TspDTI ATGAA 6 cut(s) 21, 348, 498, 1055, 1116, 1425
TspGWI ACGGA 3 cut(s) 108, 435, 1380
TspRI CASTG 1 cut(s) 1558
Tth111I GACNNNGTC 1 cut(s) 671
VneI GTGCAC 1 cut(s) 1450
VspI ATTAAT 1 cut(s) 683
XapI RAATTY 3 cut(s) 700, 1369, 1393
XhoI CTCGAG 1 cut(s) 961
XmiI GTMKAC 1 cut(s) 1400
XspI CTAG 3 cut(s) 147, 785, 1467
ZrmI AGTACT 1 cut(s) 967
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.