Rh4BG031900

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000236
Physical Location & Seq
Reverse (-)
5714160 .. 5723870
9711 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG031900.1

Sequence Viewer

Length: 897 bp
ATGTCTTATATACACTGCAATAAGAGCAGTAATGATCCCATCAAATGTGACTGCAGTTTTCAAAACCGCACGATTTGCCACGTCACAGAAATTGCACTGATGGGTAGTGGTTTGACTGGAGTTATACCTAAAGAACTAGGCAATCTCAGACATCTGAGGGAACTGGATCTCAGTAACAATCAATTGATTGGTTCTATACCCGCTAGCTTGGGAAAGTTATCTTCGCTTTACAAGCTGGATCTCAGTAACAATCAATTGACTGATTCTATACCCGCTAGCTTGGGAAATTTATCTTCACTTTCCATGCTGAATCTTTACAAAAATCAATTGACCGGATCCATTCCAGCGAGTTTGGGAAATATGAAACTTCAAGTCCTCATGGATCATACTTCGAGCCGCAATATGGCTGTTACCTTCGAACTGGATCTTTCATACAATCAATTAAGTGGATCCATACCAGTTGTCTCTGCTGTGGCATCTGGAAGGACAAATGCAGGACACAGGGGAGATAGCCAGGAAAGTGAATTTCTTCTAGACACGGCTTATGATTTACATCGAAAAGGAAGGCTGGTGGACTTGGTTGACAAAACTTTGTCTACCAAGTATGATGCAAAACAAGCCATAATCATCTTGAATTTAGCAGTAAAGTGCACCAGTATATCTCCAACACTGCGGCCTACTATGTCTGAAGTAGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTTCCCCTGCTCTTAATGATAGTCACCTTGCTCGAGTTGATTTCTCTGTTTCTATGGAAGCAACTTCGAGAGCATCCACATCATCCTATTTGATCAAAGGGGAAGATGAAACAAAACACATTTCTGAGAGTACCCCCTTGAGACTCCCAAATGAAACATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

298

Amino Acids

32.43

Weight (kDa)

6.24

Isoelectric Point (pI)

39.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 27 - 86 4.5e-09 Leucine rich repeat
LRR_14 PF23598 41 - 133 2.3e-11 Leucine-rich repeat region
LRR_4 PF12799 51 - 88 1.3e-07 Leucine Rich repeats (2 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 596
AciI CCGC 5 cut(s) 67, 201, 273, 397, 673
AclWI GGATC 9 cut(s) 29, 174, 246, 330, 343, 390, 432, 444, 457
AcsI RAATTY 3 cut(s) 286, 524, 634
AcuI CTGAAG 1 cut(s) 708
AfaI GTAC 1 cut(s) 865
AfiI CCNNNNNNNGG 1 cut(s) 403
AgsI TTSAA 3 cut(s) 62, 371, 634
AjiI CACGTC 1 cut(s) 82
AjnI CCWGG 1 cut(s) 513
AluBI AGCT 3 cut(s) 207, 235, 279
AluI AGCT 3 cut(s) 207, 235, 279
Alw21I GWGCWC 1 cut(s) 653
Alw26I GTCTC 2 cut(s) 469, 868
Alw44I GTGCAC 1 cut(s) 649
AlwI GGATC 9 cut(s) 29, 174, 246, 330, 343, 390, 432, 444, 457
Ama87I CYCGRG 1 cut(s) 765
AoxI GGCC 1 cut(s) 674
ApaLI GTGCAC 1 cut(s) 649
ApoI RAATTY 3 cut(s) 286, 524, 634
Asp700I GAANNNNTTC 1 cut(s) 528
AsuHPI GGTGA 1 cut(s) 749
AsuII TTCGAA 1 cut(s) 417
AsuNHI GCTAGC 2 cut(s) 203, 275
AvaI CYCGRG 1 cut(s) 765
BaeGI GKGCMC 1 cut(s) 653
BamHI GGATCC 2 cut(s) 335, 449
Bbv12I GWGCWC 1 cut(s) 653
BccI CCATC 2 cut(s) 47, 94
BceAI ACGGC 1 cut(s) 555
BciT130I CCWGG 1 cut(s) 515
BclI TGATCA 1 cut(s) 825
BcoDI GTCTC 2 cut(s) 469, 868
BfaI CTAG 4 cut(s) 137, 204, 276, 533
BfmI CTRYAG 1 cut(s) 52
BisI GCNGC 2 cut(s) 397, 674
BlsI GCNGC 2 cut(s) 398, 675
Bme1390I CCNGG 1 cut(s) 515
BmeT110I CYCGRG 1 cut(s) 765
BmgBI CACGTC 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 337, 451
BmrFI CCNGG 1 cut(s) 515
BmsI GCATC 3 cut(s) 485, 598, 815
BmtI GCTAGC 2 cut(s) 207, 279
BpmI CTGGAG 1 cut(s) 138
Bpu14I TTCGAA 1 cut(s) 417
BpuEI CTTGAG 1 cut(s) 892
BsaBI GATNNNNATC 1 cut(s) 552
BsaWI WCCGGW 1 cut(s) 332
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse1I ACTGG 5 cut(s) 121, 168, 426, 458, 654
Bse8I GATNNNNATC 1 cut(s) 552
BseBI CCWGG 1 cut(s) 515
BseGI GGATG 2 cut(s) 806, 815
BseJI GATNNNNATC 1 cut(s) 552
BseLI CCNNNNNNNGG 1 cut(s) 403
BseMII CTCAG 5 cut(s) 146, 160, 184, 256, 849
BseNI ACTGG 5 cut(s) 121, 168, 426, 458, 654
BseRI GAGGAG 1 cut(s) 740
BseSI GKGCMC 1 cut(s) 653
BshFI GGCC 1 cut(s) 676
BsiHKAI GWGCWC 1 cut(s) 653
BsiHKCI CYCGRG 1 cut(s) 765
BsiSI CCGG 1 cut(s) 333
BslI CCNNNNNNNGG 1 cut(s) 403
BsmAI GTCTC 2 cut(s) 469, 868
BsnI GGCC 1 cut(s) 676
BsoBI CYCGRG 1 cut(s) 765
Bsp119I TTCGAA 1 cut(s) 417
Bsp1286I GDGCHC 1 cut(s) 653
Bsp143I GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
BspACI CCGC 5 cut(s) 67, 201, 273, 397, 673
BspANI GGCC 1 cut(s) 676
BspCNI CTCAG 5 cut(s) 147, 159, 183, 255, 850
BspLI GGNNCC 2 cut(s) 337, 451
BspMAI CTGCAG 1 cut(s) 56
BspOI GCTAGC 2 cut(s) 207, 279
BspPI GGATC 9 cut(s) 29, 174, 246, 330, 343, 390, 432, 444, 457
BspT104I TTCGAA 1 cut(s) 417
BsrI ACTGG 5 cut(s) 121, 168, 426, 458, 654
BssMI GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
Bst2UI CCWGG 1 cut(s) 515
BstAPI GCANNNNNTGC 1 cut(s) 75
BstBI TTCGAA 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 205, 277
BstDEI CTNAG 5 cut(s) 146, 155, 170, 242, 858
BstF5I GGATG 2 cut(s) 806, 815
BstKTI GATC 8 cut(s) 37, 169, 241, 338, 385, 427, 452, 828
BstMAI GTCTC 2 cut(s) 469, 868
BstMBI GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
BstMWI GCNNNNNNNGC 4 cut(s) 24, 75, 232, 617
BstNI CCWGG 1 cut(s) 515
BstSCI CCNGG 1 cut(s) 513
BstSFI CTRYAG 1 cut(s) 52
BstSLI GKGCMC 1 cut(s) 653
BstX2I RGATCY 5 cut(s) 166, 238, 335, 424, 449
BstYI RGATCY 5 cut(s) 166, 238, 335, 424, 449
BsuRI GGCC 1 cut(s) 676
BtrI CACGTC 1 cut(s) 82
BtsCI GGATG 2 cut(s) 806, 815
BtsI GCAGTG 2 cut(s) 13, 668
BtsIMutI CAGTG 3 cut(s) 13, 95, 668
Cac8I GCNNGC 2 cut(s) 205, 277
Csp6I GTAC 1 cut(s) 864
CviAII CATG 3 cut(s) 304, 379, 891
CviQI GTAC 1 cut(s) 864
DdeI CTNAG 5 cut(s) 146, 155, 170, 242, 858
DpnI GATC 8 cut(s) 36, 168, 240, 337, 384, 426, 451, 827
DpnII GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
Eco57I CTGAAG 1 cut(s) 708
Eco88I CYCGRG 1 cut(s) 765
EcoRII CCWGG 1 cut(s) 513
FaeI CATG 3 cut(s) 307, 382, 894
FatI CATG 3 cut(s) 303, 378, 890
FauI CCCGC 2 cut(s) 208, 280
FbaI TGATCA 1 cut(s) 825
FblI GTMKAC 1 cut(s) 596
Fnu4HI GCNGC 2 cut(s) 397, 674
FokI GGATG 2 cut(s) 793, 802
Fsp4HI GCNGC 2 cut(s) 397, 674
FspBI CTAG 4 cut(s) 137, 204, 276, 533
GluI GCNGC 2 cut(s) 397, 674
GsuI CTGGAG 1 cut(s) 138
HaeIII GGCC 1 cut(s) 676
HapII CCGG 1 cut(s) 333
Hin1II CATG 3 cut(s) 307, 382, 894
HincII GTYRAC 1 cut(s) 583
HindII GTYRAC 1 cut(s) 583
HinfI GANTC 3 cut(s) 263, 310, 876
HpaII CCGG 1 cut(s) 333
HphI GGTGA 1 cut(s) 749
Hpy166II GTNNAC 4 cut(s) 574, 583, 597, 651
Hpy188I TCNGA 4 cut(s) 149, 156, 688, 859
Hpy188III TCNNGA 4 cut(s) 480, 533, 631, 801
Hpy8I GTNNAC 4 cut(s) 574, 583, 597, 651
HpyAV CCTTC 3 cut(s) 424, 477, 558
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 6 cut(s) 18, 54, 95, 494, 611, 651
HpyF10VI GCNNNNNNNGC 4 cut(s) 24, 75, 232, 617
HpyF3I CTNAG 5 cut(s) 146, 155, 170, 242, 858
HpySE526I ACGT 1 cut(s) 81
Hsp92II CATG 3 cut(s) 307, 382, 894
Ksp22I TGATCA 1 cut(s) 825
Kzo9I GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
LweI GCATC 3 cut(s) 485, 598, 815
MaeI CTAG 4 cut(s) 137, 204, 276, 533
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 6 cut(s) 47, 82, 173, 245, 409, 755
MalI GATC 8 cut(s) 36, 168, 240, 337, 384, 426, 451, 827
MboI GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
MboII GAAGA 4 cut(s) 213, 285, 521, 848
MfeI CAATTG 3 cut(s) 182, 254, 326
MflI RGATCY 5 cut(s) 166, 238, 335, 424, 449
MhlI GDGCHC 1 cut(s) 653
MluCI AATT 9 cut(s) 90, 182, 254, 286, 326, 440, 524, 634, 720
MlyI GAGTC 1 cut(s) 870
MmeI TCCRAC 1 cut(s) 689
MnlI CCTC 3 cut(s) 150, 386, 718
MroXI GAANNNNTTC 1 cut(s) 528
MseI TTAA 2 cut(s) 443, 747
MspI CCGG 1 cut(s) 333
MspR9I CCNGG 1 cut(s) 515
MunI CAATTG 3 cut(s) 182, 254, 326
MvaI CCWGG 1 cut(s) 515
MwoI GCNNNNNNNGC 4 cut(s) 24, 75, 232, 617
NdeII GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
NheI GCTAGC 2 cut(s) 203, 275
NlaIII CATG 3 cut(s) 307, 382, 894
NlaIV GGNNCC 2 cut(s) 337, 451
NmuCI GTSAC 3 cut(s) 47, 82, 755
NspV TTCGAA 1 cut(s) 417
PaeR7I CTCGAG 1 cut(s) 765
PdmI GAANNNNTTC 1 cut(s) 528
PfeI GAWTC 2 cut(s) 263, 310
PkrI GCNGC 2 cut(s) 398, 675
PleI GAGTC 1 cut(s) 870
PpsI GAGTC 1 cut(s) 870
Psp6I CCWGG 1 cut(s) 513
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 2 cut(s) 337, 451
PspXI VCTCGAGB 1 cut(s) 765
PsrI GAACNNNNNNTAC 2 cut(s) 684, 716
PstI CTGCAG 1 cut(s) 56
PsuI RGATCY 5 cut(s) 166, 238, 335, 424, 449
RsaI GTAC 1 cut(s) 865
RsaNI GTAC 1 cut(s) 864
SaqAI TTAA 2 cut(s) 443, 747
SatI GCNGC 2 cut(s) 397, 674
Sau3AI GATC 8 cut(s) 34, 166, 238, 335, 382, 424, 449, 825
SchI GAGTC 1 cut(s) 870
ScrFI CCNGG 1 cut(s) 515
SduI GDGCHC 1 cut(s) 653
SetI ASST 7 cut(s) 84, 130, 209, 237, 281, 416, 762
SfaNI GCATC 3 cut(s) 485, 598, 815
SfcI CTRYAG 1 cut(s) 52
Sfr274I CTCGAG 1 cut(s) 765
SfuI TTCGAA 1 cut(s) 417
SlaI CTCGAG 1 cut(s) 765
SmlI CTYRAG 2 cut(s) 765, 871
SmoI CTYRAG 2 cut(s) 765, 871
Sse9I AATT 9 cut(s) 90, 182, 254, 286, 326, 440, 524, 634, 720
SsiI CCGC 5 cut(s) 67, 201, 273, 397, 673
SspMI CTAG 4 cut(s) 137, 204, 276, 533
StyD4I CCNGG 1 cut(s) 513
TaiI ACGT 1 cut(s) 84
TaqI TCGA 5 cut(s) 392, 417, 556, 766, 800
TasI AATT 9 cut(s) 90, 182, 254, 286, 326, 440, 524, 634, 720
TauI GCSGC 2 cut(s) 399, 676
TfiI GAWTC 2 cut(s) 263, 310
Tru1I TTAA 2 cut(s) 443, 747
Tru9I TTAA 2 cut(s) 443, 747
TscAI CASTG 3 cut(s) 20, 102, 675
TseFI GTSAC 3 cut(s) 47, 82, 755
Tsp45I GTSAC 3 cut(s) 47, 82, 755
TspDTI ATGAA 3 cut(s) 377, 420, 855
TspRI CASTG 3 cut(s) 20, 102, 675
VneI GTGCAC 1 cut(s) 649
XapI RAATTY 3 cut(s) 286, 524, 634
XbaI TCTAGA 1 cut(s) 532
XcmI CCANNNNNNNNNTGG 1 cut(s) 888
XhoI CTCGAG 1 cut(s) 765
XmiI GTMKAC 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 528
XspI CTAG 4 cut(s) 137, 204, 276, 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.