Rroxscaffold_1G00075110

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000236
Physical Location & Seq
Forward (+)
95882543 .. 95892992
10450 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075110.1

Sequence Viewer

Length: 3108 bp
ATGGGTACTGAAGCTTCAGCAAGACTCGTCAGCTTTTCTCTTATAATACTATGCCTCATCTTTACTCTTTGTCAGCTTGGTTCTGAATTCAAATATCAGAGTATGGCTCAAAGGCCGCCCGAGTTGCCTTATGAAGAAGTGAGCGCTTTGGAAGAACTAGTATCGCAGTTGGATTCAGACAGTGCAATGTCCCTTGAACAGTCATATTGCAATGAAAGCAGCTATAATGGGATTGATGGCATCACATGTGATTGCAATTTTCAAAATGGATGCCACATCACAGAAATTAAACTGCGAGCTAAAGGTTTGAGCGGATCTATTATTCTTGAAGAACTAGCCAGTCTCACATATCTGAAGACACTGGATCTCAGTAGGAATCAATTGACTGGTTCTATACCTGCTAGCTTGGGGAATTTATCTTCGCTCAACATCCTGGATCTTTCTGACAATCAATTAAGTGGATCCATACCAGCCACTTTAGGAAATGTGGCGGTCTCTTCAATGATATCTGATCAAGATGCGTACCAAATGTTCAAGTCTAAGCTATATGACAACGCCTTTGTCTACCGGCTGTTCTACCTGGATCTTTCAAACAATCAATTGACTGGGACTATACCAGAGAACTTAGCGAATTTGACTTTCGCCGGGGCAATTTATCTGGGTGACAACTTTCTCAAGGGTTCTATACCAGCATCTTTGGGTAAACTGACTTATCTCGTGTCGCTGCGTTTGCATGACAATGGAATATCTGGTACTCTCCCACAAGCACTTGGAAATTTGAAAAACCTTCTATACTTTTCTGTAGATGGTAACTATTTGTCTGGCCCATTACCTGACTTCATAGCCAACTGGGCTGATATCTCTGAACTTTATCTCAACGGAAACAATTTCCACGGAAAGATACCTGCCGGAATTTTTAATATATCATATCTACGATATTTGGTAATTAGTGATGTAGGAGACTCTCATTTCCAGTTCCTACCGTCTAATATGACGAGCAGCTTACTTACTTTGATACTAAGAAACTGCTCAATCTCCGGTCAAATTCCTTCTGACATGGGAAATATGTCATGGTTATCGAACCTAGACCTGAGCTTCAACAGCTTAACGGGTGGAATCCCAGATTCTTTGAAAAACATAAGATTGAGTTTAATGTCCTTTTCAAACAATATGCTTTCTGGGAAAATTCCTGATTGGGTTCAAAATGCAACCAGGAGAGCAATATTTGTTAGGACGGACCCAACAACGGAGAAGTATTGTCCCCAGGGAAAACCTAAGTACCATTCATTGTTTATAAATTGTGGCGGTGGGGAAACACCCTTTGATGGAAATGTTTATGAACCAGATAGTGAAACGTCACAATTTTACTTGAGTCCAAAAGGAAACTGGGCTCGGAGCAGTGCTGGAAGCATCGTGGGTCTACAGAATGCTCTTAATTTTAGTAGATTCTTAAAAACCGTGAAATGTGGGCTTTCTTCTGAAGCAGCTTTATACAATAATGCTCGCACTTCTCCTGTCTCTCTAAAATATTACGGGTTCTGTTTACGTAAAGGCAAATACAATGTAACACTTCACTTTGCTGAAATTGTTGATGATGAAAAGGATTACAGAAGTACAGAGAAACGCGTATTTGATGTATATATTCAGGGTGAGAGGAAACTAAAGGATTTCAACATTATAGACAAGGCAGGACGTCCCAATAATGTATCTCAAGTTAATTTCACGGATGTCAGTGTAAATGATAGTACATTAGAGATCCACTTCTACTCGGCTGGAAAAGGGCTTGATCAGGGACCTCTAATATCTGCTATATCCGTAACTCCAGAAATAGATATAGGTCTAGAAAAGCCTGTCACCCTCAAACAATTAAAAGATGCTACTCGGAATTTTAGCAAGAGGAACGAGATTGGTCAAGGGGGTTTTGGGACCGTTTACAAGGCTGAAGTGCAAGGGAAAATTGTAGCTGTGAAGAAACTTTCCTCTCATTCAGAGGAAAGGATCAATCAATTGATAAATGAGTTTTATACCTTAAAATCAATGAGTCAAGAAAATCTTGTTCAGTTGCTGGACATCTACAACGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGCAAAACAACTCCCTTGCACACGCCTTATTTGACTCAAAGTCCAAACTGAAACTTGACTGGGAAGCTAGGTTTAACATTTGCTTGGGAATAGCTAGGGGATTGGTGTATCTACATGAGCATCCCAGGCTGAAGATGGTTCACAGAGACATTAAATCAGCTAATATTCTTCTCGATGGAAACCTCAAGGCTAAAATATCAGACTTTGGATTGGCAAGCCTTTACACCGAAGATGATCAATTCAAGTTCATCAAAGTAGAAGTGCCACAGGGATATATGGCACCTGAGTATGTTCGAGGAGTTGTGACAGCTAAAGCTGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGAAAAATGCAGGACACACGCGAGATAGCCAGGAAAGTGAATTTCTTTTAGACACGGCTTGTGATTTACAGCGAAAAGGAAAACTGGTGGACTTGGTTGATAAAACCTTGTATAACAAGTATGATGCAAAACAAGCCATCATCATCTTGAATTTAGCAGTAATGTGCATAAATATATCGCCAACTCTGAGGCCTACTATGTCTGAAGTTTTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTTCCCCTGCCCTGAAGGAGCCGATTAAGGACATCAACGACCCCCACGTGAATGAGATCGCGGAGTTCGCGGTGTCGGAGTACAACAAGAAATCCGGGAAGAAGCTGAAGTTACGGAGTGTGGTGAAGGGCGAGACTCAGGTTGTCGCAGGTGAGAATTACCGGCTCGTCATCGTCGTTGAGGATAACTCGGCGGCGGCCAAGTATGAGGGCGTTGTGTACGAGAGGATTTGGGAGCATACTAGGGAATTGCTCTCCTTCGATAGTCAAATTGCTCAAGTTGATTCCACTGTTTCTATGGAAGTAACCTCGAAAGCATCCACGTCATCCAATTTGATCAAAGTGGAAGATGAAACAGAACACATTTCTGAGAGTACCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1035

Amino Acids

114.74

Weight (kDa)

5.68

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 114 - 152 7e-06 Leucine rich repeat
LRR_4 PF12799 118 - 152 9.8e-06 Leucine Rich repeats (2 copies)
Malectin PF11721 429 - 604 8.1e-29 Malectin domain
Pkinase PF00069 631 - 897 1.6e-44 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 633 - 897 7.5e-43 Protein tyrosine and serine/threonine kinase
Cystatin PF00031 916 - 973 2e-12 Cystatin domain
SQAPI PF16845 917 - 996 6.1e-31 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 44, 1295
AarI CACCTGC 1 cut(s) 2864
AatII GACGTC 1 cut(s) 1696
Acc36I ACCTGC 3 cut(s) 406, 913, 2864
AccB1I GGYRCC 1 cut(s) 2392
AccBSI CCGCTC 1 cut(s) 312
AccI GTMKAC 3 cut(s) 564, 1420, 2436
AccII CGCG 4 cut(s) 1626, 2497, 2786, 2795
AciI CCGC 8 cut(s) 116, 312, 491, 1305, 2786, 2795, 2918, 2921
AclWI GGATC 8 cut(s) 322, 372, 444, 456, 469, 591, 1750, 2006
AcoI YGGCCR 1 cut(s) 2922
AcuI CTGAAG 8 cut(s) 30, 374, 1500, 1962, 2264, 2700, 2759, 2852
AcvI CACGTG 1 cut(s) 2773
AcyI GRCGYC 1 cut(s) 1693
AfaI GTAC 9 cut(s) 7, 524, 754, 1280, 1615, 1747, 2807, 2945, 3100
AfeI AGCGCT 1 cut(s) 145
AfiI CCNNNNNNNGG 2 cut(s) 1246, 1325
AflIII ACRYGT 2 cut(s) 245, 1624
AhdI GACNNNNNGTC 1 cut(s) 2152
AhlI ACTAGT 1 cut(s) 157
AjiI CACGTC 1 cut(s) 3048
AjnI CCWGG 6 cut(s) 432, 579, 1211, 1263, 2237, 2505
Alw26I GTCTC 6 cut(s) 347, 499, 954, 1522, 2253, 2852
AlwI GGATC 8 cut(s) 322, 372, 444, 456, 469, 591, 1750, 2006
AlwNI CAGNNNCTG 1 cut(s) 2065
Ama87I CYCGRG 1 cut(s) 119
Aor51HI AGCGCT 1 cut(s) 145
AoxI GGCC 5 cut(s) 113, 823, 2086, 2666, 2922
ApeKI GCWGC 4 cut(s) 219, 724, 999, 1484
ArsI GACNNNNNNTTYG 2 cut(s) 542, 574
AspLEI GCGC 1 cut(s) 146
AspS9I GGNCC 4 cut(s) 824, 1237, 1793, 1926
AsuC2I CCSGG 2 cut(s) 646, 2821
AsuHPI GGTGA 5 cut(s) 674, 1661, 1846, 2860, 2888
AsuNHI GCTAGC 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 119
AvaII GGWCC 3 cut(s) 1237, 1793, 1926
BaeI ACNNNNGTAYC 2 cut(s) 1262, 1295
BamHI GGATCC 1 cut(s) 461
BanI GGYRCC 1 cut(s) 2392
BanII GRGCYC 1 cut(s) 1393
BarI GAAGNNNNNNTAC 1 cut(s) 30
BauI CACGAG 1 cut(s) 716
BbrPI CACGTG 1 cut(s) 2773
BbsI GAAGAC 1 cut(s) 362
BbvI GCAGC 4 cut(s) 231, 711, 1011, 1496
BccI CCATC 6 cut(s) 230, 800, 1319, 2242, 2282, 2621
BceAI ACGGC 1 cut(s) 2547
BciT130I CCWGG 6 cut(s) 434, 581, 1213, 1265, 2239, 2507
BclI TGATCA 4 cut(s) 511, 1786, 2347, 3060
BcnI CCSGG 2 cut(s) 646, 2821
BcoDI GTCTC 6 cut(s) 347, 499, 954, 1522, 2253, 2852
BcuI ACTAGT 1 cut(s) 157
BfaI CTAG 9 cut(s) 158, 335, 402, 1085, 1841, 2181, 2208, 2967, 3106
BfmI CTRYAG 3 cut(s) 801, 1421, 2437
BfoI RGCGCY 1 cut(s) 147
BfuAI ACCTGC 3 cut(s) 406, 913, 2864
BglI GCCNNNNNGGC 1 cut(s) 851
BisI GCNGC 7 cut(s) 116, 220, 725, 1000, 1485, 2919, 2922
BlsI GCNGC 7 cut(s) 117, 221, 726, 1001, 1486, 2920, 2923
Bme1390I CCNGG 8 cut(s) 434, 581, 646, 1213, 1265, 2239, 2507, 2821
Bme18I GGWCC 3 cut(s) 1237, 1793, 1926
BmeRI GACNNNNNGTC 1 cut(s) 2152
BmeT110I CYCGRG 1 cut(s) 119
BmgBI CACGTC 1 cut(s) 3048
BmgT120I GGNCC 4 cut(s) 824, 1237, 1793, 1926
BmiI GGNNCC 6 cut(s) 463, 1239, 1794, 1927, 2394, 2745
BmrFI CCNGG 8 cut(s) 434, 581, 646, 1213, 1265, 2239, 2507, 2821
BmrI ACTGGG 4 cut(s) 615, 859, 1396, 2182
BmsI GCATC 9 cut(s) 249, 260, 508, 701, 1419, 1864, 2242, 2590, 3050
BmtI GCTAGC 1 cut(s) 405
BmuI ACTGGG 4 cut(s) 615, 859, 1396, 2182
BpiI GAAGAC 1 cut(s) 362
BplI GAGNNNNNCTC 4 cut(s) 91, 123, 2897, 2929
BpmI CTGGAG 1 cut(s) 1806
Bpu10I CCTNAGC 1 cut(s) 1091
BpuEI CTTGAG 5 cut(s) 659, 1390, 1695, 2282, 2985
BpuMI CCSGG 2 cut(s) 646, 2821
BsaAI YACGTR 2 cut(s) 1547, 2773
BsaHI GRCGYC 1 cut(s) 1693
BsaI GGTCTC 1 cut(s) 499
BsaJI CCNNGG 5 cut(s) 645, 892, 1263, 1264, 2237
BsaWI WCCGGW 1 cut(s) 1037
Bsc4I CCNNNNNNNGG 2 cut(s) 1246, 1325
Bse118I RCCGGY 2 cut(s) 567, 2886
Bse1I ACTGG 9 cut(s) 339, 366, 391, 610, 854, 973, 1391, 2177, 2565
Bse3DI GCAATG 2 cut(s) 192, 217
BseBI CCWGG 6 cut(s) 434, 581, 1213, 1265, 2239, 2507
BseDI CCNNGG 5 cut(s) 645, 892, 1263, 1264, 2237
BseGI GGATG 6 cut(s) 275, 429, 1732, 2233, 3041, 3050
BseLI CCNNNNNNNGG 2 cut(s) 1246, 1325
BseMI GCAATG 2 cut(s) 192, 217
BseMII CTCAG 6 cut(s) 382, 1082, 2388, 2654, 2876, 3084
BseNI ACTGG 9 cut(s) 339, 366, 391, 610, 854, 973, 1391, 2177, 2565
BseRI GAGGAG 2 cut(s) 2424, 2732
BseXI GCAGC 4 cut(s) 231, 711, 1011, 1496
Bsh1236I CGCG 4 cut(s) 1626, 2497, 2786, 2795
BshFI GGCC 5 cut(s) 115, 825, 2088, 2668, 2924
BshNI GGYRCC 1 cut(s) 2392
BsiHKCI CYCGRG 1 cut(s) 119
BsiSI CCGG 6 cut(s) 568, 645, 909, 1038, 2820, 2887
BslFI GGGAC 6 cut(s) 175, 622, 1245, 1680, 1806, 1939
BslI CCNNNNNNNGG 2 cut(s) 1246, 1325
BsmAI GTCTC 6 cut(s) 347, 499, 954, 1522, 2253, 2852
BsmFI GGGAC 6 cut(s) 175, 622, 1245, 1680, 1806, 1939
BsmI GAATGC 1 cut(s) 1432
BsnI GGCC 5 cut(s) 115, 825, 2088, 2668, 2924
Bso31I GGTCTC 1 cut(s) 499
BsoBI CYCGRG 1 cut(s) 119
Bsp1286I GDGCHC 1 cut(s) 1393
BspACI CCGC 8 cut(s) 116, 312, 491, 1305, 2786, 2795, 2918, 2921
BspANI GGCC 5 cut(s) 115, 825, 2088, 2668, 2924
BspCNI CTCAG 6 cut(s) 381, 1083, 2389, 2655, 2875, 3085
BspFNI CGCG 4 cut(s) 1626, 2497, 2786, 2795
BspLI GGNNCC 6 cut(s) 463, 1239, 1794, 1927, 2394, 2745
BspMI ACCTGC 3 cut(s) 406, 913, 2864
BspOI GCTAGC 1 cut(s) 405
BspPI GGATC 8 cut(s) 322, 372, 444, 456, 469, 591, 1750, 2006
BspT107I GGYRCC 1 cut(s) 2392
BspTNI GGTCTC 1 cut(s) 499
BsrBI CCGCTC 1 cut(s) 312
BsrDI GCAATG 2 cut(s) 192, 217
BsrFI RCCGGY 2 cut(s) 567, 2886
BsrI ACTGG 9 cut(s) 339, 366, 391, 610, 854, 973, 1391, 2177, 2565
BssAI RCCGGY 2 cut(s) 567, 2886
BssECI CCNNGG 5 cut(s) 645, 892, 1263, 1264, 2237
BssNI GRCGYC 1 cut(s) 1693
BssSI CACGAG 1 cut(s) 716
Bst2BI CACGAG 1 cut(s) 716
Bst2UI CCWGG 6 cut(s) 434, 581, 1213, 1265, 2239, 2507
Bst4CI ACNGT 8 cut(s) 182, 201, 984, 1459, 1930, 2441, 2467, 3016
Bst6I CTCTTC 1 cut(s) 502
BstACI GRCGYC 1 cut(s) 1693
BstBAI YACGTR 2 cut(s) 1547, 2773
BstC8I GCNNGC 5 cut(s) 297, 403, 1504, 2090, 2329
BstDSI CCRYGG 1 cut(s) 892
BstF5I GGATG 6 cut(s) 275, 429, 1732, 2233, 3041, 3050
BstFNI CGCG 4 cut(s) 1626, 2497, 2786, 2795
BstH2I RGCGCY 1 cut(s) 147
BstHHI GCGC 1 cut(s) 146
BstMAI GTCTC 6 cut(s) 347, 499, 954, 1522, 2253, 2852
BstMWI GCNNNNNNNGC 8 cut(s) 124, 216, 730, 851, 1101, 2239, 2609, 2792
BstNI CCWGG 6 cut(s) 434, 581, 1213, 1265, 2239, 2507
BstNSI RCATGY 1 cut(s) 249
BstSCI CCNGG 8 cut(s) 432, 579, 644, 1211, 1263, 2237, 2505, 2819
BstSFI CTRYAG 3 cut(s) 801, 1421, 2437
BstSNI TACGTA 1 cut(s) 1547
BstUI CGCG 4 cut(s) 1626, 2497, 2786, 2795
BstV1I GCAGC 4 cut(s) 231, 711, 1011, 1496
BstV2I GAAGAC 1 cut(s) 362
BstX2I RGATCY 6 cut(s) 314, 364, 436, 461, 583, 1755
BstYI RGATCY 6 cut(s) 314, 364, 436, 461, 583, 1755
BsuRI GGCC 5 cut(s) 115, 825, 2088, 2668, 2924
BtgI CCRYGG 1 cut(s) 892
BtrI CACGTC 1 cut(s) 3048
BtsCI GGATG 6 cut(s) 275, 429, 1732, 2233, 3041, 3050
BtsI GCAGTG 1 cut(s) 1405
BtsIMutI CAGTG 5 cut(s) 187, 359, 1405, 1738, 3012
BveI ACCTGC 3 cut(s) 406, 913, 2864
Cac8I GCNNGC 5 cut(s) 297, 403, 1504, 2090, 2329
CaiI CAGNNNCTG 1 cut(s) 2065
CfoI GCGC 1 cut(s) 146
Cfr10I RCCGGY 2 cut(s) 567, 2886
Cfr13I GGNCC 4 cut(s) 824, 1237, 1793, 1926
Csp6I GTAC 9 cut(s) 6, 523, 753, 1279, 1614, 1746, 2806, 2944, 3099
CviAII CATG 5 cut(s) 246, 734, 1057, 1071, 2228
CviQI GTAC 9 cut(s) 6, 523, 753, 1279, 1614, 1746, 2806, 2944, 3099
DriI GACNNNNNGTC 1 cut(s) 2152
EaeI YGGCCR 1 cut(s) 2922
Eam1104I CTCTTC 1 cut(s) 502
Eam1105I GACNNNNNGTC 1 cut(s) 2152
EarI CTCTTC 1 cut(s) 502
Eco105I TACGTA 1 cut(s) 1547
Eco147I AGGCCT 2 cut(s) 2088, 2668
Eco24I GRGCYC 1 cut(s) 1393
Eco31I GGTCTC 1 cut(s) 499
Eco32I GATATC 2 cut(s) 507, 859
Eco47I GGWCC 3 cut(s) 1237, 1793, 1926
Eco47III AGCGCT 1 cut(s) 145
Eco57I CTGAAG 8 cut(s) 30, 374, 1500, 1962, 2264, 2700, 2759, 2852
Eco72I CACGTG 1 cut(s) 2773
Eco88I CYCGRG 1 cut(s) 119
EcoO109I RGGNCCY 1 cut(s) 1793
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 6 cut(s) 432, 579, 1211, 1263, 2237, 2505
EcoRV GATATC 2 cut(s) 507, 859
EcoT38I GRGCYC 1 cut(s) 1393
FaeI CATG 5 cut(s) 249, 737, 1060, 1074, 2231
FalI AAGNNNNNCTT 2 cut(s) 2037, 2069
FaqI GGGAC 6 cut(s) 175, 622, 1245, 1680, 1806, 1939
FatI CATG 5 cut(s) 245, 733, 1056, 1070, 2227
FbaI TGATCA 4 cut(s) 511, 1786, 2347, 3060
FblI GTMKAC 3 cut(s) 564, 1420, 2436
Fnu4HI GCNGC 7 cut(s) 116, 220, 725, 1000, 1485, 2919, 2922
FokI GGATG 6 cut(s) 282, 416, 1739, 2220, 3028, 3037
FriOI GRGCYC 1 cut(s) 1393
Fsp4HI GCNGC 7 cut(s) 116, 220, 725, 1000, 1485, 2919, 2922
FspBI CTAG 9 cut(s) 158, 335, 402, 1085, 1841, 2181, 2208, 2967, 3106
GlaI GCGC 1 cut(s) 145
GluI GCNGC 7 cut(s) 116, 220, 725, 1000, 1485, 2919, 2922
GsuI CTGGAG 1 cut(s) 1806
HaeII RGCGCY 1 cut(s) 147
HaeIII GGCC 5 cut(s) 115, 825, 2088, 2668, 2924
HapII CCGG 6 cut(s) 568, 645, 909, 1038, 2820, 2887
HhaI GCGC 1 cut(s) 146
Hin1I GRCGYC 1 cut(s) 1693
Hin1II CATG 5 cut(s) 249, 737, 1060, 1074, 2231
Hin6I GCGC 1 cut(s) 144
HinP1I GCGC 1 cut(s) 144
HindIII AAGCTT 1 cut(s) 12
HpaII CCGG 6 cut(s) 568, 645, 909, 1038, 2820, 2887
HphI GGTGA 5 cut(s) 674, 1661, 1846, 2860, 2888
Hpy166II GTNNAC 9 cut(s) 565, 704, 1421, 1544, 1933, 2254, 2437, 2566, 2944
Hpy188III TCNNGA 8 cut(s) 326, 515, 1190, 1823, 1841, 2045, 2285, 2623
Hpy8I GTNNAC 9 cut(s) 565, 704, 1421, 1544, 1933, 2254, 2437, 2566, 2944
Hpy99I CGWCG 1 cut(s) 2903
HpyAV CCTTC 6 cut(s) 797, 1059, 2469, 2734, 2845, 2992
HpyCH4III ACNGT 8 cut(s) 182, 201, 984, 1459, 1930, 2441, 2467, 3016
HpyCH4IV ACGT 5 cut(s) 1355, 1546, 1693, 2772, 3047
HpyF10VI GCNNNNNNNGC 8 cut(s) 124, 216, 730, 851, 1101, 2239, 2609, 2792
HpySE526I ACGT 5 cut(s) 1355, 1546, 1693, 2772, 3047
Hsp92I GRCGYC 1 cut(s) 1693
Hsp92II CATG 5 cut(s) 249, 737, 1060, 1074, 2231
HspAI GCGC 1 cut(s) 144
Ksp22I TGATCA 4 cut(s) 511, 1786, 2347, 3060
LmnI GCTCC 3 cut(s) 1395, 2743, 2959
Lsp1109I GCAGC 4 cut(s) 231, 711, 1011, 1496
LweI GCATC 9 cut(s) 249, 260, 508, 701, 1419, 1864, 2242, 2590, 3050
MaeI CTAG 9 cut(s) 158, 335, 402, 1085, 1841, 2181, 2208, 2967, 3106
MaeII ACGT 5 cut(s) 1355, 1546, 1693, 2772, 3047
MaeIII GTNAC 9 cut(s) 662, 809, 1356, 1564, 1816, 1852, 2416, 2835, 3028
MbiI CCGCTC 1 cut(s) 312
MfeI CAATTG 3 cut(s) 380, 599, 2006
MflI RGATCY 6 cut(s) 314, 364, 436, 461, 583, 1755
MhlI GDGCHC 1 cut(s) 1393
MluI ACGCGT 1 cut(s) 1624
MlyI GAGTC 6 cut(s) 18, 956, 1381, 2050, 2141, 2854
MmeI TCCRAC 2 cut(s) 150, 2781
MslI CAYNNNNRTG 2 cut(s) 738, 2775
MspI CCGG 6 cut(s) 568, 645, 909, 1038, 2820, 2887
MspR9I CCNGG 8 cut(s) 434, 581, 646, 1213, 1265, 2239, 2507, 2821
MunI CAATTG 3 cut(s) 380, 599, 2006
Mva1269I GAATGC 1 cut(s) 1432
MvaI CCWGG 6 cut(s) 434, 581, 1213, 1265, 2239, 2507
MvnI CGCG 4 cut(s) 1626, 2497, 2786, 2795
MwoI GCNNNNNNNGC 8 cut(s) 124, 216, 730, 851, 1101, 2239, 2609, 2792
NciI CCSGG 2 cut(s) 646, 2821
NheI GCTAGC 1 cut(s) 401
NlaIII CATG 5 cut(s) 249, 737, 1060, 1074, 2231
NlaIV GGNNCC 6 cut(s) 463, 1239, 1794, 1927, 2394, 2745
NmeAIII GCCGAG 2 cut(s) 1748, 2894
NmuCI GTSAC 4 cut(s) 662, 1356, 1852, 2416
NspI RCATGY 1 cut(s) 249
PaqCI CACCTGC 1 cut(s) 2864
PasI CCCWGGG 1 cut(s) 1264
PceI AGGCCT 2 cut(s) 2088, 2668
PciI ACATGT 1 cut(s) 245
PcsI WCGNNNNNNNCGW 2 cut(s) 2769, 2898
PctI GAATGC 1 cut(s) 1432
PfeI GAWTC 6 cut(s) 173, 376, 1116, 1124, 1446, 3008
PfoI TCCNGGA 2 cut(s) 432, 2819
PkrI GCNGC 7 cut(s) 117, 221, 726, 1001, 1486, 2920, 2923
PleI GAGTC 6 cut(s) 18, 956, 1380, 2049, 2141, 2854
PmaCI CACGTG 1 cut(s) 2773
PmlI CACGTG 1 cut(s) 2773
PpsI GAGTC 6 cut(s) 18, 956, 1380, 2049, 2141, 2854
Ppu21I YACGTR 2 cut(s) 1547, 2773
PpuMI RGGWCCY 1 cut(s) 1793
PscI ACATGT 1 cut(s) 245
PsiI TTATAA 2 cut(s) 44, 1295
Psp5II RGGWCCY 1 cut(s) 1793
Psp6I CCWGG 6 cut(s) 432, 579, 1211, 1263, 2237, 2505
PspCI CACGTG 1 cut(s) 2773
PspGI CCWGG 6 cut(s) 432, 579, 1211, 1263, 2237, 2505
PspN4I GGNNCC 6 cut(s) 463, 1239, 1794, 1927, 2394, 2745
PspPI GGNCC 4 cut(s) 824, 1237, 1793, 1926
PspPPI RGGWCCY 1 cut(s) 1793
PsrI GAACNNNNNNTAC 2 cut(s) 515, 547
PstNI CAGNNNCTG 1 cut(s) 2065
PsuI RGATCY 6 cut(s) 314, 364, 436, 461, 583, 1755
RsaI GTAC 9 cut(s) 7, 524, 754, 1280, 1615, 1747, 2807, 2945, 3100
RsaNI GTAC 9 cut(s) 6, 523, 753, 1279, 1614, 1746, 2806, 2944, 3099
RseI CAYNNNNRTG 2 cut(s) 738, 2775
SatI GCNGC 7 cut(s) 116, 220, 725, 1000, 1485, 2919, 2922
Sau96I GGNCC 4 cut(s) 824, 1237, 1793, 1926
SchI GAGTC 6 cut(s) 18, 956, 1381, 2050, 2141, 2854
ScrFI CCNGG 8 cut(s) 434, 581, 646, 1213, 1265, 2239, 2507, 2821
SduI GDGCHC 1 cut(s) 1393
SfaNI GCATC 9 cut(s) 249, 260, 508, 701, 1419, 1864, 2242, 2590, 3050
SfcI CTRYAG 3 cut(s) 801, 1421, 2437
SinI GGWCC 3 cut(s) 1237, 1793, 1926
SmiMI CAYNNNNRTG 2 cut(s) 738, 2775
SmlI CTYRAG 5 cut(s) 674, 1369, 1710, 2297, 3000
SmoI CTYRAG 5 cut(s) 674, 1369, 1710, 2297, 3000
SnaBI TACGTA 1 cut(s) 1547
SpeI ACTAGT 1 cut(s) 157
SseBI AGGCCT 2 cut(s) 2088, 2668
SsiI CCGC 8 cut(s) 116, 312, 491, 1305, 2786, 2795, 2918, 2921
SspI AATATT 3 cut(s) 1224, 1529, 2278
SspMI CTAG 9 cut(s) 158, 335, 402, 1085, 1841, 2181, 2208, 2967, 3106
StuI AGGCCT 2 cut(s) 2088, 2668
StyD4I CCNGG 8 cut(s) 432, 579, 644, 1211, 1263, 2237, 2505, 2819
TaaI ACNGT 8 cut(s) 182, 201, 984, 1459, 1930, 2441, 2467, 3016
TaiI ACGT 5 cut(s) 1358, 1549, 1696, 2775, 3050
TaqI TCGA 5 cut(s) 1079, 2286, 2407, 2985, 3035
TatI WGTACW 3 cut(s) 1613, 1745, 2805
TauI GCSGC 3 cut(s) 118, 2921, 2924
TfiI GAWTC 6 cut(s) 173, 376, 1116, 1124, 1446, 3008
TscAI CASTG 5 cut(s) 187, 366, 1405, 1738, 3019
TseFI GTSAC 4 cut(s) 662, 1356, 1852, 2416
TseI GCWGC 4 cut(s) 219, 724, 999, 1484
Tsp45I GTSAC 4 cut(s) 662, 1356, 1852, 2416
TspDTI ATGAA 9 cut(s) 147, 228, 829, 1275, 1353, 1611, 2121, 2350, 3090
TspGWI ACGGA 7 cut(s) 894, 909, 1250, 1262, 1739, 1804, 2854
TspRI CASTG 5 cut(s) 187, 366, 1405, 1738, 3019
VpaK11BI GGWCC 3 cut(s) 1237, 1793, 1926
XbaI TCTAGA 1 cut(s) 1840
XceI RCATGY 1 cut(s) 249
XcmI CCANNNNNNNNNTGG 3 cut(s) 1383, 2245, 3019
XmiI GTMKAC 3 cut(s) 564, 1420, 2436
XspI CTAG 9 cut(s) 158, 335, 402, 1085, 1841, 2181, 2208, 2967, 3106
ZraI GACGTC 1 cut(s) 1694
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.