Rw5G025480

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000236
Physical Location & Seq
Reverse (-)
36399276 .. 36416999
17724 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G025480.1

Sequence Viewer

Length: 3465 bp
ATGGGTACTGAGGCTTCAGCAAGACTTGTTAGTAAATTTACAATTCTCTGCCTCATCTTTACTCTTTGGCAGCTTGGTTCTGAATTCAAAAATCGAAGTATAGCTCAAACGTTGCCCGATGAAGAAGTGAGAGCTTTTAAGCAAGTAGCAAAAAAGTTGGGGTTAAACCTTGGATATCCTGGAGTGTTCTTAGATGGCTCATTCTGCAACCAGAGCTTATTTCTTGGCATCGGCTGTAACTGCACTTTTCAAAACGACACCATCTGCCATATCAGAAAAATTGAACTGAGTGCTAGCGGTTTGACTGGAGTTATACCTGATGAACTGGCCAATCTCACACATCTAGAGAGCCTGGATCTCAGTATGAATCAATTGACTGGTTCCATACCTGCTAGCTTGGGGAATTTATCTTGGCTCAATGACCTGGATATTTCAAACAATCAATTGATCGGGTCCATTCCAGAGAGTTTGGGGAATATAAAATTTCAAGCGCACTACTCCGATAAGAAGGTTGACATGATTCGGGGATACTTTAATCTCGATCTTTCAAGCAATCAATTGAGTGGATCCATACCCGTCACTTTAGGGAAGTTGGCTGTCCCATCGGCTGCCGACAGCGGATCGCCCCTAGACCTCTCCCTGGATCTTTCAAACAATCAATTGACTGGGCCCATACCAGAGAGCTTAGGGAATTTGACTTTTGGGACCATATATATTTATCTGTACGACAACTTTCTCAGCGGCTCTATACCAGCATCTTTGGGTGCATTGTCTCATCTCTGGACACTGGATTTGCATAACAATGGAATCTTTGGTACTCTCCCACAGACACTTGGAAATTTGACATACCTTGAACACTTCACGGTCGGATCCAACAGTATCACCGGGACATTACCAGAAAGTTTTGCCAGCCTTCAATCGCTGACGACATTTTCTGTAGCTGGTAACTATTTGTCTGGCCCTTTACCTGACTTCATAGCCAACTGGACTGAAATCGAAGAACTTTATCTCAACGGAAACAATTTTCACGGAAGGATACCTGCCGGAATTTTTAATATATCGTATCTACAAATTTTGGCAATAAGTGACTTCGGAGCAGACTCTAATTTCCAGTTCCCACCATCTACACATGTGACGACAAGTTTTATTCATCGTATTCTAATACTAAGAAACTGCTCAATCACTGGTCAAATTCCTTCTTACATTGGAAATATGTCATCATTATCGAACCTAGACTTGAGCTTCAACAACTTAACGGGTAGAATCCCAGATGCTTTGAAAAACTTAAATTTAAGTTACATATCTTTTTCAAACAATATGCTTTCTGGGGAAATTCCTGATTGGATTCAAAATGCAAACTGGAGTAAGATTGATCTTTCATACAATAATTTTTCAAAAGCAACATTTGAAAAATCGTCCAAGTTAGACTGGAATTTGTTTTCCTGCTGCTGCAACTTATCAACCTGTCTGCCAAATACAACGGACCCAACCACGGAGAAGTATTGTCCAAGGCCTAATTACCATTCATTGTTTATAAATTGCGGTGGTGGGGAACCGATTAAAGTTGATGGAAATGCATATGATCAAGATAATGATACATCACAGTTTTACCTAAGTCCAAAAGGAAACTGGGCTCGAAGTAGTGCTGGATGCACCGTTGATGCCTTCAATTCCAGTGAATTCTTAAAAAGCATGAAATGTGGGCTATCCTCTGAAGCAACTTTATACAAGAGTGCTCGCAGTTCTCCGGTCTCTCTAAAATATTACGGGTTCTGTTTACGTAAAGGCAAATACAGGGTAATACTTCATTTTGCTGAAATTGTTTGCGAGGATATGAGGTACAAAAGTACAGATAAACGCATATTTGATGTATATATTCAGGGTGAGAGGAAACTAAAGGATTTCAACATTATAGAGAAGGCAGGAGGTCCGAATTTAGTACATGAAGAAAATTTCACAGCGGTTAATGTAAATGATGGTGTATTAGAGATACACTTCTACTCGGCTGGAAAAGGGACTCTTCAGGGACCTCTCATATCTGCTATATCCGTAACTCCAGAGTACAAGCTACACAAACAACTATCTCCTTTGCATGTAGCGCTCATTGCTGTGGCCTCAATCATAGCTTTCGTATTGCTTTTATTGCTTTTTGCCTGGATGATGGGATGGCTGGGTACAGATCACTTACAAGAAATAGATATAGGTCTAGAAAAGCCCGTCACCCTCAAACAATTAAAAGTTGCTACAGGGAATTTTAGCAAGAGGAACGAGATTGGTCAAGGGGGTTTTGGGACCGTTTACAAGGCTGAACTGCAAGGAAAAATTGTAGCTGTGAAGAAACTTTCCTCTCATTCAGAGGAAAGGATAAATCAGTTGAAAAATGAGTTTTATGCTTTGAAATCAATGAGTCAAGAGAACCTTGTTCAGTTACTGGACGTTTACAATGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGCAAAACAAATCCCTTGCACACGCTTTATTTGACTCAAAGTCCAAATTGAAACTTGATTGGGAAGCTAGGTTTAACATTTGCTTGGGAATAGCTAGGGGATTGGCGTATCTACATGAGCATCCCAGGCTGAAGACGGTTCACAGGGACATTAAATCAGCAAATATTCTTCTCGATGGAAACCTCAAGGCTAAAATATCAGACTTTGGATTGGCAAGCCTTTACACCGAAGATGATCAATTCAAGTTCGTCAAAGTAGAAGTGACAGAGGGATATATGGCACCCGAGTATGTTCGAGGAGTTGTGACATCTAAAGCTGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGAAAAATGCAGGACACAAGCGAGATAGCCAGGAAACTGAATTTCTTTTAGACACGGCTTGTGATTTACAGCGAAAAGGAAAGTTGGTGGACTTGGTTGATAAAACCTTGTCTAACAAGTATGATGCAAAACAAGCCATCATCATCTTGAATTTAGCAGTAAAGTGCATCAATATATCGCCAACTCTGAGGCCTACTATGTCTGAAGTTGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATCTGTTCCTCTGATCAGATTGAGGACATCAACGACCCCCATGTGAAGGAGATTGCAGAGTTCGCAGTGTCGGAGTACAACAAGAAATCTGGGAAGAAGCTGGAGTTACAGAGCGTGGTGAAGGGCGAGACTCGGGTCGTCCCCGGCGAGAATTATCGGCTCGTCATCGCCGTCAAGGATAACTCGGCGGCGGCTAAGTACGAGGGCGTTGTGTATGAGAGGATTTGGGAGCATACTAGGGAATTGCTCTCCTTCGATAGTCACATTGCACAAGTTGATTCCTCTGTTTCTATGGAAGTAAGCTCGAGAGCATCCACACCATCCAATTTGATCAAAGGGGAAGATGAAACAGAACACATTTCTGAGACTATCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

1154

Amino Acids

127.63

Weight (kDa)

5.89

Isoelectric Point (pI)

35.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 90 - 149 4.4e-07 Leucine rich repeat
LRR_4 PF12799 113 - 154 9.5e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 253 - 337 5.5e-07 Leucine-rich repeat region
LRR_8 PF13855 259 - 318 1.2e-06 Leucine rich repeat
Malectin PF11721 509 - 681 7.7e-31 Malectin domain
Pkinase PF00069 753 - 1018 3.6e-47 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 755 - 1020 3e-42 Protein tyrosine and serine/threonine kinase
SQAPI PF16845 1036 - 1115 7.7e-30 Aspartic acid proteinase inhibitor
Cystatin PF00031 1036 - 1093 1.4e-11 Cystatin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1535
Acc36I ACCTGC 2 cut(s) 397, 1048
AccB1I GGYRCC 1 cut(s) 2758
AccI GTMKAC 1 cut(s) 2802
AciI CCGC 7 cut(s) 297, 618, 741, 1542, 1961, 3275, 3278
AclI AACGTT 1 cut(s) 110
AclWI GGATC 7 cut(s) 363, 561, 574, 628, 651, 864, 877
AcoI YGGCCR 1 cut(s) 327
AcuI CTGAAG 4 cut(s) 1734, 2006, 2630, 3066
AfeI AGCGCT 1 cut(s) 2100
AfiI CCNNNNNNNGG 3 cut(s) 640, 1492, 3133
AflIII ACRYGT 1 cut(s) 1129
AhdI GACNNNNNGTC 1 cut(s) 2518
AjnI CCWGG 7 cut(s) 178, 351, 423, 639, 2153, 2603, 2871
Alw21I GWGCWC 1 cut(s) 1738
Alw26I GTCTC 4 cut(s) 777, 1756, 3209, 3446
AlwI GGATC 7 cut(s) 363, 561, 574, 628, 651, 864, 877
AlwNI CAGNNNCTG 1 cut(s) 2431
Ama87I CYCGRG 3 cut(s) 2762, 3219, 3391
Aor51HI AGCGCT 1 cut(s) 2100
AoxI GGCC 7 cut(s) 327, 668, 958, 1511, 2112, 2452, 3032
ApaI GGGCCC 1 cut(s) 672
ApeKI GCWGC 4 cut(s) 70, 608, 1446, 1449
ArsI GACNNNNNNTTYG 2 cut(s) 775, 807
AspLEI GCGC 2 cut(s) 493, 2101
AspS9I GGNCC 9 cut(s) 453, 668, 669, 705, 959, 1483, 1928, 2027, 2292
AsuC2I CCSGG 2 cut(s) 886, 3231
AsuHPI GGTGA 4 cut(s) 874, 1895, 2212, 3217
AsuNHI GCTAGC 2 cut(s) 293, 392
AvaI CYCGRG 3 cut(s) 2762, 3219, 3391
AvaII GGWCC 6 cut(s) 453, 705, 1483, 1928, 2027, 2292
BaeGI GKGCMC 1 cut(s) 672
BaeI ACNNNNGTAYC 2 cut(s) 1831, 1864
BalI TGGCCA 1 cut(s) 329
BamHI GGATCC 2 cut(s) 566, 869
BanI GGYRCC 1 cut(s) 2758
BanII GRGCYC 2 cut(s) 672, 1636
BarI GAAGNNNNNNTAC 1 cut(s) 30
BbsI GAAGAC 1 cut(s) 2618
Bbv12I GWGCWC 1 cut(s) 1738
BbvI GCAGC 4 cut(s) 82, 595, 1433, 1436
BceAI ACGGC 2 cut(s) 2913, 3242
BciT130I CCWGG 7 cut(s) 180, 353, 425, 641, 2155, 2605, 2873
BciVI GTATCC 2 cut(s) 521, 1029
BclI TGATCA 4 cut(s) 1582, 2713, 3100, 3417
BcnI CCSGG 2 cut(s) 886, 3231
BcoDI GTCTC 4 cut(s) 777, 1756, 3209, 3446
BfmI CTRYAG 3 cut(s) 936, 2244, 2803
BfoI RGCGCY 1 cut(s) 2102
BfuAI ACCTGC 2 cut(s) 397, 1048
BfuI GTATCC 2 cut(s) 521, 1029
BglII AGATCT 1 cut(s) 3087
BisI GCNGC 7 cut(s) 71, 609, 742, 1447, 1450, 3276, 3279
BlsI GCNGC 7 cut(s) 72, 610, 743, 1448, 1451, 3277, 3280
Bme1390I CCNGG 9 cut(s) 180, 353, 425, 641, 886, 2155, 2605, 2873, 3231
Bme18I GGWCC 6 cut(s) 453, 705, 1483, 1928, 2027, 2292
BmeRI GACNNNNNGTC 1 cut(s) 2518
BmeT110I CYCGRG 3 cut(s) 2762, 3219, 3391
BmgT120I GGNCC 9 cut(s) 453, 668, 669, 705, 959, 1483, 1928, 2027, 2292
BmrFI CCNGG 9 cut(s) 180, 353, 425, 641, 886, 2155, 2605, 2873, 3231
BmrI ACTGGG 2 cut(s) 675, 1639
BmsI GCATC 9 cut(s) 237, 764, 1261, 1640, 1651, 2608, 2956, 3018, 3407
BmtI GCTAGC 2 cut(s) 297, 396
BmuI ACTGGG 2 cut(s) 675, 1639
BoxI GACNNNNGTC 1 cut(s) 3221
BpiI GAAGAC 1 cut(s) 2618
BpmI CTGGAG 5 cut(s) 201, 327, 1381, 2040, 3209
Bpu10I CCTNAGC 1 cut(s) 685
BpuEI CTTGAG 2 cut(s) 1258, 2648
BpuMI CCSGG 2 cut(s) 886, 3231
BsaAI YACGTR 1 cut(s) 1781
BsaI GGTCTC 1 cut(s) 1756
BsaJI CCNNGG 6 cut(s) 169, 639, 1491, 1508, 2603, 3229
BsaWI WCCGGW 1 cut(s) 1747
Bsc4I CCNNNNNNNGG 3 cut(s) 640, 1492, 3133
Bse3DI GCAATG 2 cut(s) 2103, 3351
BseBI CCWGG 7 cut(s) 180, 353, 425, 641, 2155, 2605, 2873
BseDI CCNNGG 6 cut(s) 169, 639, 1491, 1508, 2603, 3229
BseGI GGATG 6 cut(s) 1655, 2163, 2171, 2599, 3398, 3407
BseLI CCNNNNNNNGG 3 cut(s) 640, 1492, 3133
BseMI GCAATG 2 cut(s) 2103, 3351
BseMII CTCAG 5 cut(s) 278, 373, 751, 3020, 3441
BseRI GAGGAG 2 cut(s) 2790, 3098
BseSI GKGCMC 1 cut(s) 672
BseXI GCAGC 4 cut(s) 82, 595, 1433, 1436
BseYI CCCAGC 1 cut(s) 2170
BsgI GTGCAG 1 cut(s) 226
Bsh1285I CGRYCG 1 cut(s) 867
BshFI GGCC 7 cut(s) 329, 670, 960, 1513, 2114, 2454, 3034
BshNI GGYRCC 1 cut(s) 2758
BsiEI CGRYCG 1 cut(s) 867
BsiHKAI GWGCWC 1 cut(s) 1738
BsiHKCI CYCGRG 3 cut(s) 2762, 3219, 3391
BsiSI CCGG 4 cut(s) 885, 1044, 1748, 3231
BslFI GGGAC 8 cut(s) 584, 718, 901, 2029, 2040, 2305, 2639, 3212
BslI CCNNNNNNNGG 3 cut(s) 640, 1492, 3133
BsmAI GTCTC 4 cut(s) 777, 1756, 3209, 3446
BsmFI GGGAC 8 cut(s) 584, 718, 901, 2029, 2040, 2305, 2639, 3212
BsnI GGCC 7 cut(s) 329, 670, 960, 1513, 2114, 2454, 3034
Bso31I GGTCTC 1 cut(s) 1756
BsoBI CYCGRG 3 cut(s) 2762, 3219, 3391
Bsp120I GGGCCC 1 cut(s) 668
Bsp1286I GDGCHC 3 cut(s) 672, 1636, 1738
BspACI CCGC 7 cut(s) 297, 618, 741, 1542, 1961, 3275, 3278
BspANI GGCC 7 cut(s) 329, 670, 960, 1513, 2114, 2454, 3034
BspCNI CTCAG 5 cut(s) 279, 372, 750, 3021, 3442
BspMI ACCTGC 2 cut(s) 397, 1048
BspOI GCTAGC 2 cut(s) 297, 396
BspPI GGATC 7 cut(s) 363, 561, 574, 628, 651, 864, 877
BspT107I GGYRCC 1 cut(s) 2758
BspTNI GGTCTC 1 cut(s) 1756
BsrDI GCAATG 2 cut(s) 2103, 3351
BssECI CCNNGG 6 cut(s) 169, 639, 1491, 1508, 2603, 3229
BssT1I CCWWGG 2 cut(s) 169, 1508
Bst2UI CCWGG 7 cut(s) 180, 353, 425, 641, 2155, 2605, 2873
Bst4CI ACNGT 8 cut(s) 865, 878, 1605, 1657, 2296, 2617, 2807, 2833
Bst6I CTCTTC 1 cut(s) 2025
BstBAI YACGTR 1 cut(s) 1781
BstC8I GCNNGC 6 cut(s) 295, 394, 910, 1738, 2456, 2695
BstDSI CCRYGG 1 cut(s) 1491
BstF5I GGATG 6 cut(s) 1655, 2163, 2171, 2599, 3398, 3407
BstH2I RGCGCY 1 cut(s) 2102
BstHHI GCGC 2 cut(s) 493, 2101
BstMAI GTCTC 4 cut(s) 777, 1756, 3209, 3446
BstMCI CGRYCG 1 cut(s) 867
BstMWI GCNNNNNNNGC 9 cut(s) 204, 213, 240, 2098, 2105, 2143, 2605, 2975, 3149
BstNI CCWGG 7 cut(s) 180, 353, 425, 641, 2155, 2605, 2873
BstNSI RCATGY 2 cut(s) 1133, 2096
BstPAI GACNNNNGTC 1 cut(s) 3221
BstSCI CCNGG 9 cut(s) 178, 351, 423, 639, 884, 2153, 2603, 2871, 3229
BstSFI CTRYAG 3 cut(s) 936, 2244, 2803
BstSLI GKGCMC 1 cut(s) 672
BstSNI TACGTA 1 cut(s) 1781
BstV1I GCAGC 4 cut(s) 82, 595, 1433, 1436
BstV2I GAAGAC 1 cut(s) 2618
BstX2I RGATCY 5 cut(s) 355, 566, 643, 869, 3087
BstYI RGATCY 5 cut(s) 355, 566, 643, 869, 3087
BsuI GTATCC 2 cut(s) 521, 1029
BsuRI GGCC 7 cut(s) 329, 670, 960, 1513, 2114, 2454, 3034
BtgI CCRYGG 1 cut(s) 1491
BtgZI GCGATG 1 cut(s) 3238
BtsCI GGATG 6 cut(s) 1655, 2163, 2171, 2599, 3398, 3407
BtsI GCAGTG 1 cut(s) 3159
BtsIMutI CAGTG 4 cut(s) 785, 1182, 1681, 3159
BveI ACCTGC 2 cut(s) 397, 1048
Cac8I GCNNGC 6 cut(s) 295, 394, 910, 1738, 2456, 2695
CaiI CAGNNNCTG 1 cut(s) 2431
CfoI GCGC 2 cut(s) 493, 2101
Cfr13I GGNCC 9 cut(s) 453, 668, 669, 705, 959, 1483, 1928, 2027, 2292
CviAII CATG 7 cut(s) 517, 1130, 1693, 1943, 2093, 2594, 3128
DriI GACNNNNNGTC 1 cut(s) 2518
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 1 cut(s) 2025
Eam1105I GACNNNNNGTC 1 cut(s) 2518
EarI CTCTTC 1 cut(s) 2025
Eco105I TACGTA 1 cut(s) 1781
Eco130I CCWWGG 2 cut(s) 169, 1508
Eco147I AGGCCT 3 cut(s) 1513, 2454, 3034
Eco24I GRGCYC 2 cut(s) 672, 1636
Eco31I GGTCTC 1 cut(s) 1756
Eco32I GATATC 1 cut(s) 176
Eco47I GGWCC 6 cut(s) 453, 705, 1483, 1928, 2027, 2292
Eco47III AGCGCT 1 cut(s) 2100
Eco57I CTGAAG 4 cut(s) 1734, 2006, 2630, 3066
Eco88I CYCGRG 3 cut(s) 2762, 3219, 3391
EcoO109I RGGNCCY 1 cut(s) 2027
EcoRI GAATTC 2 cut(s) 83, 1679
EcoRII CCWGG 7 cut(s) 178, 351, 423, 639, 2153, 2603, 2871
EcoRV GATATC 1 cut(s) 176
EcoT14I CCWWGG 2 cut(s) 169, 1508
EcoT22I ATGCAT 1 cut(s) 1579
EcoT38I GRGCYC 2 cut(s) 672, 1636
ErhI CCWWGG 2 cut(s) 169, 1508
FaeI CATG 7 cut(s) 520, 1133, 1696, 1946, 2096, 2597, 3131
FalI AAGNNNNNCTT 4 cut(s) 2004, 2036, 2403, 2435
FaqI GGGAC 8 cut(s) 584, 718, 901, 2029, 2040, 2305, 2639, 3212
FatI CATG 7 cut(s) 516, 1129, 1692, 1942, 2092, 2593, 3127
FauNDI CATATG 1 cut(s) 1579
FbaI TGATCA 4 cut(s) 1582, 2713, 3100, 3417
FblI GTMKAC 1 cut(s) 2802
Fnu4HI GCNGC 7 cut(s) 71, 609, 742, 1447, 1450, 3276, 3279
FokI GGATG 6 cut(s) 1662, 2170, 2178, 2586, 3385, 3394
FriOI GRGCYC 2 cut(s) 672, 1636
Fsp4HI GCNGC 7 cut(s) 71, 609, 742, 1447, 1450, 3276, 3279
GlaI GCGC 2 cut(s) 492, 2100
GluI GCNGC 7 cut(s) 71, 609, 742, 1447, 1450, 3276, 3279
GsaI CCCAGC 1 cut(s) 2174
GsuI CTGGAG 5 cut(s) 201, 327, 1381, 2040, 3209
HaeII RGCGCY 1 cut(s) 2102
HaeIII GGCC 7 cut(s) 329, 670, 960, 1513, 2114, 2454, 3034
HapII CCGG 4 cut(s) 885, 1044, 1748, 3231
HhaI GCGC 2 cut(s) 493, 2101
Hin1II CATG 7 cut(s) 520, 1133, 1696, 1946, 2096, 2597, 3131
Hin6I GCGC 2 cut(s) 491, 2099
HinP1I GCGC 2 cut(s) 491, 2099
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HpaII CCGG 4 cut(s) 885, 1044, 1748, 3231
HphI GGTGA 4 cut(s) 874, 1895, 2212, 3217
Hpy166II GTNNAC 7 cut(s) 514, 1778, 2299, 2440, 2620, 2803, 2932
Hpy8I GTNNAC 7 cut(s) 514, 1778, 2299, 2440, 2620, 2803, 2932
HpyCH4III ACNGT 8 cut(s) 865, 878, 1605, 1657, 2296, 2617, 2807, 2833
HpyCH4IV ACGT 3 cut(s) 110, 1780, 2436
HpyF10VI GCNNNNNNNGC 9 cut(s) 204, 213, 240, 2098, 2105, 2143, 2605, 2975, 3149
HpySE526I ACGT 3 cut(s) 110, 1780, 2436
Hsp92II CATG 7 cut(s) 520, 1133, 1696, 1946, 2096, 2597, 3131
HspAI GCGC 2 cut(s) 491, 2099
Ksp22I TGATCA 4 cut(s) 1582, 2713, 3100, 3417
LmnI GCTCC 2 cut(s) 1094, 3316
Lsp1109I GCAGC 4 cut(s) 82, 595, 1433, 1436
LweI GCATC 9 cut(s) 237, 764, 1261, 1640, 1651, 2608, 2956, 3018, 3407
MaeII ACGT 3 cut(s) 110, 1780, 2436
MfeI CAATTG 4 cut(s) 371, 443, 557, 659
MflI RGATCY 5 cut(s) 355, 566, 643, 869, 3087
MhlI GDGCHC 3 cut(s) 672, 1636, 1738
MlsI TGGCCA 1 cut(s) 329
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 5 cut(s) 1094, 2011, 2416, 2507, 3211
MmeI TCCRAC 3 cut(s) 847, 897, 3138
Mox20I TGGCCA 1 cut(s) 329
Mph1103I ATGCAT 1 cut(s) 1579
MscI TGGCCA 1 cut(s) 329
MslI CAYNNNNRTG 2 cut(s) 801, 2108
Msp20I TGGCCA 1 cut(s) 329
MspA1I CMGCKG 3 cut(s) 618, 741, 1961
MspI CCGG 4 cut(s) 885, 1044, 1748, 3231
MspR9I CCNGG 9 cut(s) 180, 353, 425, 641, 886, 2155, 2605, 2873, 3231
MunI CAATTG 4 cut(s) 371, 443, 557, 659
MvaI CCWGG 7 cut(s) 180, 353, 425, 641, 2155, 2605, 2873
MwoI GCNNNNNNNGC 9 cut(s) 204, 213, 240, 2098, 2105, 2143, 2605, 2975, 3149
NciI CCSGG 2 cut(s) 886, 3231
NdeI CATATG 1 cut(s) 1579
NheI GCTAGC 2 cut(s) 293, 392
NlaIII CATG 7 cut(s) 520, 1133, 1696, 1946, 2096, 2597, 3131
NmeAIII GCCGAG 2 cut(s) 1982, 3251
NmuCI GTSAC 7 cut(s) 577, 1085, 1132, 2218, 2740, 2782, 3347
NsiI ATGCAT 1 cut(s) 1579
NspI RCATGY 2 cut(s) 1133, 2096
PaeR7I CTCGAG 1 cut(s) 3391
PceI AGGCCT 3 cut(s) 1513, 2454, 3034
PciI ACATGT 1 cut(s) 1129
PcsI WCGNNNNNNNCGW 2 cut(s) 3231, 3255
PfeI GAWTC 6 cut(s) 367, 520, 807, 1263, 1345, 3365
PfoI TCCNGGA 1 cut(s) 178
PkrI GCNGC 7 cut(s) 72, 610, 743, 1448, 1451, 3277, 3280
PleI GAGTC 5 cut(s) 1094, 2011, 2415, 2507, 3211
PpsI GAGTC 5 cut(s) 1094, 2011, 2415, 2507, 3211
Ppu21I YACGTR 1 cut(s) 1781
PpuMI RGGWCCY 1 cut(s) 2027
PscI ACATGT 1 cut(s) 1129
PshAI GACNNNNGTC 1 cut(s) 3221
PsiI TTATAA 1 cut(s) 1535
Psp1406I AACGTT 1 cut(s) 110
Psp5II RGGWCCY 1 cut(s) 2027
Psp6I CCWGG 7 cut(s) 178, 351, 423, 639, 2153, 2603, 2871
PspFI CCCAGC 1 cut(s) 2170
PspGI CCWGG 7 cut(s) 178, 351, 423, 639, 2153, 2603, 2871
PspOMI GGGCCC 1 cut(s) 668
PspPI GGNCC 9 cut(s) 453, 668, 669, 705, 959, 1483, 1928, 2027, 2292
PspPPI RGGWCCY 1 cut(s) 2027
PstNI CAGNNNCTG 1 cut(s) 2431
PsuI RGATCY 5 cut(s) 355, 566, 643, 869, 3087
RseI CAYNNNNRTG 2 cut(s) 801, 2108
SatI GCNGC 7 cut(s) 71, 609, 742, 1447, 1450, 3276, 3279
Sau96I GGNCC 9 cut(s) 453, 668, 669, 705, 959, 1483, 1928, 2027, 2292
SchI GAGTC 5 cut(s) 1094, 2011, 2416, 2507, 3211
ScrFI CCNGG 9 cut(s) 180, 353, 425, 641, 886, 2155, 2605, 2873, 3231
SduI GDGCHC 3 cut(s) 672, 1636, 1738
SfaNI GCATC 9 cut(s) 237, 764, 1261, 1640, 1651, 2608, 2956, 3018, 3407
SfcI CTRYAG 3 cut(s) 936, 2244, 2803
Sfr274I CTCGAG 1 cut(s) 3391
SinI GGWCC 6 cut(s) 453, 705, 1483, 1928, 2027, 2292
SlaI CTCGAG 1 cut(s) 3391
SmiMI CAYNNNNRTG 2 cut(s) 801, 2108
SmlI CTYRAG 3 cut(s) 1237, 2663, 3391
SmoI CTYRAG 3 cut(s) 1237, 2663, 3391
SnaBI TACGTA 1 cut(s) 1781
SseBI AGGCCT 3 cut(s) 1513, 2454, 3034
SsiI CCGC 7 cut(s) 297, 618, 741, 1542, 1961, 3275, 3278
SspI AATATT 2 cut(s) 1763, 2644
StuI AGGCCT 3 cut(s) 1513, 2454, 3034
StyD4I CCNGG 9 cut(s) 178, 351, 423, 639, 884, 2153, 2603, 2871, 3229
StyI CCWWGG 2 cut(s) 169, 1508
TaaI ACNGT 8 cut(s) 865, 878, 1605, 1657, 2296, 2617, 2807, 2833
TaiI ACGT 3 cut(s) 113, 1783, 2439
TaqI TCGA 9 cut(s) 94, 540, 996, 1226, 1636, 2652, 2773, 3342, 3392
TatI WGTACW 4 cut(s) 1847, 1939, 2061, 3162
TauI GCSGC 3 cut(s) 744, 3278, 3281
TfiI GAWTC 6 cut(s) 367, 520, 807, 1263, 1345, 3365
TscAI CASTG 4 cut(s) 792, 1189, 1681, 3159
TseFI GTSAC 7 cut(s) 577, 1085, 1132, 2218, 2740, 2782, 3347
TseI GCWGC 4 cut(s) 70, 608, 1446, 1449
Tsp45I GTSAC 7 cut(s) 577, 1085, 1132, 2218, 2740, 2782, 3347
TspGWI ACGGA 5 cut(s) 1029, 1044, 1496, 1508, 2038
TspRI CASTG 4 cut(s) 792, 1189, 1681, 3159
VpaK11BI GGWCC 6 cut(s) 453, 705, 1483, 1928, 2027, 2292
XbaI TCTAGA 2 cut(s) 343, 2206
XceI RCATGY 2 cut(s) 1133, 2096
XcmI CCANNNNNNNNNTGG 1 cut(s) 1626
XhoI CTCGAG 1 cut(s) 3391
XmiI GTMKAC 1 cut(s) 2802
Zsp2I ATGCAT 1 cut(s) 1579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.