RchiOBHm_Chr5g0001251

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000236
Physical Location & Seq
Reverse (-)
669953 .. 676149
6197 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ28270

Sequence Viewer

Length: 2346 bp
ATGTTACTTCGATCAAAAAACTATTCGAATATAAGGATACTAAGAAACTGCTCAATCACTGGTCAAATTCCTTCCTACATTGGAAATATGTCATCATTAAGGAGCCTAGACCTGAGCTTCAACAGCTTAACGGGTAGAATCCCAGATTCTTTGAAAAACATAAATTTAAGTTGGATGTCTTTTCCAAACAATATGCTGACTGGGGAAATTCCTAATTGGATTCAAACTGCAGTCTGGAGTAAGATGGATCTTTCATACAATAATTTTTCAAAACCAACCTTTGAAACACCGTCCAGCTTAGACTTGAATTTGTTTTCTTGTTGTTGTAACTCCTCAATCTGTCTGCCAAATACGTATGACTGGGCGGACCCAACCACGGAGAAGTATTGTGCCGGAGGAAAACCTAATTACCATTCATTGTTTATAAATTGTGGTGGTGGGACACTTAACAACGTTGATGGAAATATTTATGATCAAGATAATGAGACATCGCAGTTTCACCGGAGTCTGAAAGGAAACTGGGCTCGAATTAGTGCTGGAAACACCGTCGACCTTGACTATGCTTCCAATCCAAGTAAAGTTTTAAAAAGTGTGAGATGTGGGCTTTCCTCTGAAGCACCTTTATACGATAGAGCTCGCACTTCCCCTGTCTCTCTAAAATATTACGGGTTCTGTTTACGTAAAGGCAAATACAATGTAACACTTCACTTTGCTGAAATTGTTGATGAGGATAACAATTATAGAAATACAACTAAGCGCGTATTTGATGTATATATACAGGGTGAGAGGAAACTAAAGGATTTCAACATTATAGAAGAGGCAGGAGGTCCGAATACAAAACATACAGAAAATTTCAGGGCTGTTAATGTAAATGATAGTACATTAGAGATCCACTTCTACTCGGCTGGAAAAGGGACTCTTGATCAGGGACCTCTCATATCTGCTATATCCGTAACTCCAGAGTACAAGCTACACGAACAACTATCTCCTTCGCACATAGCGCTCATTACTGTGGCTTCAATCATAGTTTTCGTATTGCTTGTATTGCTTTTTGCCTGGATGATGGGATGGCTGGGTACAGATCACTTACAAGAAATAGATATAGGTCAAGAAAAGCCTATCACCCTCAAACAATTAAAAGATGCTACTGGGAATTTTAGCAAGAGGAACGAGATTGGTCAAGGGGGTTTTGGGACAGTTTACAAGGCTGAAGTGCAAGGGAAAATTGTAGCTGTGAAGAAACTTTCCTCTCATTCAGAGGAAAGGATCAATCAATTGATAAATGAGTTTTATACCTTAAAATCAATGAGTCAAGAGAATCTTGTTCAGTTACTGGACATCTACAACGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGCAAAACAACTCCCTTGCACACGCCTTATTTGACTCAAAGTCCAAACTGAAACTTGATTGGGAAGCTAGGTTTAATATTTGCTTGGGAATAGCTAGGGGATTGGTGTATCTACATGAGCATCCCAGGCTGAAGATGGTTCACAGAGACATTAAATCAGCTAATATTCTTCTCGATGGAAACCTCAAGGCTAAAATATCAGACTTTGGATTGGCAAGCCTTTACACAGAAGATGATCAATTCAAGTTCATCAAAGTAGAAGTGCCACAGGGATATATGGCACCTGAGTATGTTCGAGGAGTTGTGACAGCTAAAGCTGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGAAAAATGCAGGACACACGCGAGATAGCCAGGAAAGTGAATTTCTTTTAGACACGGCTTGTGATTTACAGCGAAAAGGAAAACTGGTGGACTTGGTTGATAAAACCTTGTCTAACAAGTATGATGCAAAACAAGCCATCATCATCTTGAATTTAGCAGTAATGTGCATAAATATATCGCCAACTCTGAGGCCTACTATGTCTGAAGTTTTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTTCCCCTGCTCTGAAGGAGCCGATTAAGGACATCAACGACCCCCACGTGAATGAGATCGCGGAGTTCGCGGTGTTGGAGTATAACAAGAAATCCGGGAAGAAGCTGAAGTTACGGAGCGTGGTGAAGGGCGAGACTCAGGTTGTCGCAGGTGAGAATTACCGGCTCGTCATCCTCGTCGAGGATAACTCAGCGGCGGCCAAGTATGAGGGCGTTGTGTATGAGAGGATTTGGGAGCATACTAGGGAATTGCTCTCCTTCGATAGTCACATTGCTCAAGTTGATTCCTCTGTTTCTATGGAAGTAACCTCGAAAGAATCCACATCATCCAATTTGATCAAAAAAAGAAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

781

Amino Acids

87.71

Weight (kDa)

7.54

Isoelectric Point (pI)

39.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 139 - 315 2.2e-29 Malectin domain
Pkinase PF00069 387 - 653 5.5e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 389 - 654 3.3e-43 Protein tyrosine and serine/threonine kinase
Cystatin PF00031 672 - 729 3.7e-11 Cystatin domain
SQAPI PF16845 673 - 752 2.6e-30 Aspartic acid proteinase inhibitor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 425
AarI CACCTGC 1 cut(s) 2132
Acc36I ACCTGC 1 cut(s) 2132
AccB1I GGYRCC 1 cut(s) 1660
AccI GTMKAC 2 cut(s) 549, 1704
AccII CGCG 4 cut(s) 759, 1765, 2054, 2063
AciI CCGC 5 cut(s) 365, 2054, 2063, 2186, 2189
AclI AACGTT 1 cut(s) 453
AclWI GGATC 3 cut(s) 255, 883, 1274
AcoI YGGCCR 1 cut(s) 2190
AcsI RAATTY 8 cut(s) 66, 163, 207, 307, 850, 1153, 1784, 1894
AcuI CTGAAG 6 cut(s) 633, 1230, 1532, 1968, 2027, 2120
AcvI CACGTG 1 cut(s) 2041
AfaI GTAC 3 cut(s) 880, 965, 1078
AfeI AGCGCT 1 cut(s) 1002
AfiI CCNNNNNNNGG 2 cut(s) 376, 2173
AhdI GACNNNNNGTC 1 cut(s) 1420
AjnI CCWGG 3 cut(s) 1055, 1505, 1773
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 4 cut(s) 479, 655, 1521, 2120
AlwI GGATC 3 cut(s) 255, 883, 1274
AlwNI CAGNNNCTG 1 cut(s) 1333
Aor51HI AGCGCT 1 cut(s) 1002
AoxI GGCC 3 cut(s) 1354, 1934, 2190
ApoI RAATTY 8 cut(s) 66, 163, 207, 307, 850, 1153, 1784, 1894
AspLEI GCGC 2 cut(s) 759, 1003
AspS9I GGNCC 3 cut(s) 367, 827, 929
AsuC2I CCSGG 1 cut(s) 2089
AsuHPI GGTGA 5 cut(s) 491, 794, 1114, 2128, 2156
AsuII TTCGAA 1 cut(s) 26
AvaII GGWCC 3 cut(s) 367, 827, 929
BanI GGYRCC 1 cut(s) 1660
BanII GRGCYC 2 cut(s) 526, 637
BbrPI CACGTG 1 cut(s) 2041
Bbv12I GWGCWC 1 cut(s) 637
BccI CCATC 7 cut(s) 238, 452, 1057, 1062, 1510, 1550, 1889
BceAI ACGGC 1 cut(s) 1815
BciT130I CCWGG 3 cut(s) 1057, 1507, 1775
BciVI GTATCC 1 cut(s) 30
BclI TGATCA 4 cut(s) 472, 922, 1615, 2328
BcnI CCSGG 1 cut(s) 2089
BcoDI GTCTC 4 cut(s) 479, 655, 1521, 2120
BfaI CTAG 4 cut(s) 107, 1449, 1476, 2235
BfmI CTRYAG 2 cut(s) 228, 1705
BfoI RGCGCY 1 cut(s) 1004
BfuAI ACCTGC 1 cut(s) 2132
BfuI GTATCC 1 cut(s) 30
BisI GCNGC 2 cut(s) 2187, 2190
BlsI GCNGC 2 cut(s) 2188, 2191
Bme1390I CCNGG 4 cut(s) 1057, 1507, 1775, 2089
Bme18I GGWCC 3 cut(s) 367, 827, 929
BmeRI GACNNNNNGTC 1 cut(s) 1420
BmgT120I GGNCC 3 cut(s) 367, 827, 929
BmiI GGNNCC 5 cut(s) 104, 369, 930, 1662, 2013
BmrFI CCNGG 4 cut(s) 1057, 1507, 1775, 2089
BmrI ACTGGG 4 cut(s) 210, 370, 529, 1158
BmsI GCATC 3 cut(s) 1132, 1510, 1858
BmuI ACTGGG 4 cut(s) 210, 370, 529, 1158
BplI GAGNNNNNCTC 2 cut(s) 2165, 2197
BpmI CTGGAG 2 cut(s) 256, 942
Bpu10I CCTNAGC 1 cut(s) 113
Bpu14I TTCGAA 1 cut(s) 26
BpuEI CTTGAG 2 cut(s) 1550, 2253
BpuMI CCSGG 1 cut(s) 2089
BsaAI YACGTR 3 cut(s) 354, 680, 2041
BsaJI CCNNGG 2 cut(s) 375, 1505
BsaWI WCCGGW 1 cut(s) 501
Bsc4I CCNNNNNNNGG 2 cut(s) 376, 2173
Bse118I RCCGGY 1 cut(s) 2154
Bse1I ACTGG 7 cut(s) 64, 205, 365, 524, 1153, 1338, 1833
Bse3DI GCAATG 1 cut(s) 2262
BseBI CCWGG 3 cut(s) 1057, 1507, 1775
BseDI CCNNGG 2 cut(s) 375, 1505
BseGI GGATG 6 cut(s) 180, 1065, 1073, 1501, 2163, 2318
BseLI CCNNNNNNNGG 2 cut(s) 376, 2173
BseMI GCAATG 1 cut(s) 2262
BseMII CTCAG 5 cut(s) 104, 1656, 1922, 2144, 2196
BseNI ACTGG 7 cut(s) 64, 205, 365, 524, 1153, 1338, 1833
BseRI GAGGAG 3 cut(s) 322, 1692, 2000
BseYI CCCAGC 1 cut(s) 1072
Bsh1236I CGCG 4 cut(s) 759, 1765, 2054, 2063
BshFI GGCC 3 cut(s) 1356, 1936, 2192
BshNI GGYRCC 1 cut(s) 1660
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 4 cut(s) 393, 502, 2088, 2155
BslFI GGGAC 4 cut(s) 454, 928, 942, 1207
BslI CCNNNNNNNGG 2 cut(s) 376, 2173
BsmAI GTCTC 4 cut(s) 479, 655, 1521, 2120
BsmFI GGGAC 4 cut(s) 454, 928, 942, 1207
BsnI GGCC 3 cut(s) 1356, 1936, 2192
Bsp119I TTCGAA 1 cut(s) 26
Bsp1286I GDGCHC 2 cut(s) 526, 637
BspACI CCGC 5 cut(s) 365, 2054, 2063, 2186, 2189
BspANI GGCC 3 cut(s) 1356, 1936, 2192
BspCNI CTCAG 5 cut(s) 105, 1657, 1923, 2143, 2195
BspFNI CGCG 4 cut(s) 759, 1765, 2054, 2063
BspLI GGNNCC 5 cut(s) 104, 369, 930, 1662, 2013
BspMAI CTGCAG 1 cut(s) 232
BspMI ACCTGC 1 cut(s) 2132
BspPI GGATC 3 cut(s) 255, 883, 1274
BspT104I TTCGAA 1 cut(s) 26
BspT107I GGYRCC 1 cut(s) 1660
BsrDI GCAATG 1 cut(s) 2262
BsrFI RCCGGY 1 cut(s) 2154
BsrI ACTGG 7 cut(s) 64, 205, 365, 524, 1153, 1338, 1833
BssAI RCCGGY 1 cut(s) 2154
BssECI CCNNGG 2 cut(s) 375, 1505
Bst2UI CCWGG 3 cut(s) 1057, 1507, 1775
Bst4CI ACNGT 6 cut(s) 291, 547, 1012, 1198, 1709, 1735
Bst6I CTCTTC 1 cut(s) 810
BstBAI YACGTR 3 cut(s) 354, 680, 2041
BstBI TTCGAA 1 cut(s) 26
BstC8I GCNNGC 3 cut(s) 637, 1358, 1597
BstDEI CTNAG 8 cut(s) 41, 113, 298, 753, 1665, 1931, 2130, 2182
BstDSI CCRYGG 1 cut(s) 375
BstENI CCTNNNNNAGG 1 cut(s) 2171
BstF5I GGATG 6 cut(s) 180, 1065, 1073, 1501, 2163, 2318
BstFNI CGCG 4 cut(s) 759, 1765, 2054, 2063
BstH2I RGCGCY 1 cut(s) 1004
BstHHI GCGC 2 cut(s) 759, 1003
BstMAI GTCTC 4 cut(s) 479, 655, 1521, 2120
BstMWI GCNNNNNNNGC 6 cut(s) 123, 1000, 1045, 1507, 1877, 2060
BstNI CCWGG 3 cut(s) 1057, 1507, 1775
BstSCI CCNGG 4 cut(s) 1055, 1505, 1773, 2087
BstSFI CTRYAG 2 cut(s) 228, 1705
BstSNI TACGTA 2 cut(s) 354, 680
BstUI CGCG 4 cut(s) 759, 1765, 2054, 2063
BstX2I RGATCY 2 cut(s) 247, 888
BstYI RGATCY 2 cut(s) 247, 888
BsuI GTATCC 1 cut(s) 30
BsuRI GGCC 3 cut(s) 1356, 1936, 2192
BtgI CCRYGG 1 cut(s) 375
BtgZI GCGATG 1 cut(s) 474
BtsCI GGATG 6 cut(s) 180, 1065, 1073, 1501, 2163, 2318
BtsIMutI CAGTG 1 cut(s) 57
BveI ACCTGC 1 cut(s) 2132
Cac8I GCNNGC 3 cut(s) 637, 1358, 1597
CaiI CAGNNNCTG 1 cut(s) 1333
CfoI GCGC 2 cut(s) 759, 1003
Cfr10I RCCGGY 1 cut(s) 2154
Cfr13I GGNCC 3 cut(s) 367, 827, 929
Csp6I GTAC 3 cut(s) 879, 964, 1077
CviAII CATG 1 cut(s) 1496
CviQI GTAC 3 cut(s) 879, 964, 1077
DdeI CTNAG 8 cut(s) 41, 113, 298, 753, 1665, 1931, 2130, 2182
DraI TTTAAA 1 cut(s) 585
DriI GACNNNNNGTC 1 cut(s) 1420
EaeI YGGCCR 1 cut(s) 2190
Eam1104I CTCTTC 1 cut(s) 810
Eam1105I GACNNNNNGTC 1 cut(s) 1420
EarI CTCTTC 1 cut(s) 810
EciI GGCGGA 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 635
Eco105I TACGTA 2 cut(s) 354, 680
Eco147I AGGCCT 2 cut(s) 1356, 1936
Eco24I GRGCYC 2 cut(s) 526, 637
Eco47I GGWCC 3 cut(s) 367, 827, 929
Eco47III AGCGCT 1 cut(s) 1002
Eco53kI GAGCTC 1 cut(s) 635
Eco57I CTGAAG 6 cut(s) 633, 1230, 1532, 1968, 2027, 2120
Eco72I CACGTG 1 cut(s) 2041
EcoICRI GAGCTC 1 cut(s) 635
EcoNI CCTNNNNNAGG 1 cut(s) 2171
EcoO109I RGGNCCY 1 cut(s) 929
EcoRII CCWGG 3 cut(s) 1055, 1505, 1773
EcoT38I GRGCYC 2 cut(s) 526, 637
FaeI CATG 1 cut(s) 1499
FalI AAGNNNNNCTT 4 cut(s) 903, 935, 1305, 1337
FaqI GGGAC 4 cut(s) 454, 928, 942, 1207
FatI CATG 1 cut(s) 1495
FbaI TGATCA 4 cut(s) 472, 922, 1615, 2328
FblI GTMKAC 2 cut(s) 549, 1704
Fnu4HI GCNGC 2 cut(s) 2187, 2190
FokI GGATG 6 cut(s) 187, 1072, 1080, 1488, 2150, 2305
FriOI GRGCYC 2 cut(s) 526, 637
Fsp4HI GCNGC 2 cut(s) 2187, 2190
FspBI CTAG 4 cut(s) 107, 1449, 1476, 2235
GlaI GCGC 2 cut(s) 758, 1002
GluI GCNGC 2 cut(s) 2187, 2190
GsaI CCCAGC 1 cut(s) 1076
GsuI CTGGAG 2 cut(s) 256, 942
HaeII RGCGCY 1 cut(s) 1004
HaeIII GGCC 3 cut(s) 1356, 1936, 2192
HapII CCGG 4 cut(s) 393, 502, 2088, 2155
HhaI GCGC 2 cut(s) 759, 1003
Hin1II CATG 1 cut(s) 1499
Hin6I GCGC 2 cut(s) 757, 1001
HinP1I GCGC 2 cut(s) 757, 1001
HincII GTYRAC 1 cut(s) 550
HindII GTYRAC 1 cut(s) 550
HpaII CCGG 4 cut(s) 393, 502, 2088, 2155
HphI GGTGA 5 cut(s) 491, 794, 1114, 2128, 2156
Hpy166II GTNNAC 6 cut(s) 550, 677, 1201, 1522, 1705, 1834
Hpy188I TCNGA 8 cut(s) 510, 613, 831, 1258, 1582, 1932, 1948, 2007
Hpy188III TCNNGA 8 cut(s) 235, 476, 920, 959, 1109, 1313, 1553, 1891
Hpy8I GTNNAC 6 cut(s) 550, 677, 1201, 1522, 1705, 1834
Hpy99I CGWCG 2 cut(s) 551, 2174
HpyAV CCTTC 6 cut(s) 81, 999, 1737, 2002, 2113, 2260
HpyCH4III ACNGT 6 cut(s) 291, 547, 1012, 1198, 1709, 1735
HpyCH4IV ACGT 4 cut(s) 353, 453, 679, 2040
HpyCH4V TGCA 8 cut(s) 230, 1216, 1360, 1384, 1400, 1754, 1871, 1911
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 1000, 1045, 1507, 1877, 2060
HpyF3I CTNAG 8 cut(s) 41, 113, 298, 753, 1665, 1931, 2130, 2182
HpySE526I ACGT 4 cut(s) 353, 453, 679, 2040
Hsp92II CATG 1 cut(s) 1499
HspAI GCGC 2 cut(s) 757, 1001
Ksp22I TGATCA 4 cut(s) 472, 922, 1615, 2328
LmnI GCTCC 4 cut(s) 102, 2011, 2109, 2227
LweI GCATC 3 cut(s) 1132, 1510, 1858
MaeI CTAG 4 cut(s) 107, 1449, 1476, 2235
MaeII ACGT 4 cut(s) 353, 453, 679, 2040
MaeIII GTNAC 9 cut(s) 3, 326, 697, 952, 1329, 1684, 2103, 2258, 2296
MboII GAAGA 6 cut(s) 827, 1249, 1525, 1541, 1622, 2104
MfeI CAATTG 1 cut(s) 1274
MflI RGATCY 2 cut(s) 247, 888
MhlI GDGCHC 2 cut(s) 526, 637
MlyI GAGTC 5 cut(s) 514, 910, 1318, 1409, 2122
MmeI TCCRAC 2 cut(s) 152, 2049
MslI CAYNNNNRTG 2 cut(s) 1010, 2043
MspA1I CMGCKG 1 cut(s) 2186
MspI CCGG 4 cut(s) 393, 502, 2088, 2155
MspR9I CCNGG 4 cut(s) 1057, 1507, 1775, 2089
MunI CAATTG 1 cut(s) 1274
MvaI CCWGG 3 cut(s) 1057, 1507, 1775
MvnI CGCG 4 cut(s) 759, 1765, 2054, 2063
MwoI GCNNNNNNNGC 6 cut(s) 123, 1000, 1045, 1507, 1877, 2060
NciI CCSGG 1 cut(s) 2089
NlaIII CATG 1 cut(s) 1499
NlaIV GGNNCC 5 cut(s) 104, 369, 930, 1662, 2013
NmeAIII GCCGAG 1 cut(s) 881
NmuCI GTSAC 2 cut(s) 1684, 2258
NspV TTCGAA 1 cut(s) 26
PaqCI CACCTGC 1 cut(s) 2132
PceI AGGCCT 2 cut(s) 1356, 1936
PcsI WCGNNNNNNNCGW 2 cut(s) 2037, 2166
PfeI GAWTC 6 cut(s) 138, 146, 220, 1318, 2276, 2309
PfoI TCCNGGA 1 cut(s) 2087
PkrI GCNGC 2 cut(s) 2188, 2191
PleI GAGTC 5 cut(s) 513, 910, 1317, 1409, 2122
PmaCI CACGTG 1 cut(s) 2041
PmlI CACGTG 1 cut(s) 2041
PpsI GAGTC 5 cut(s) 513, 910, 1317, 1409, 2122
Ppu21I YACGTR 3 cut(s) 354, 680, 2041
PpuMI RGGWCCY 1 cut(s) 929
PsiI TTATAA 1 cut(s) 425
Psp124BI GAGCTC 1 cut(s) 637
Psp1406I AACGTT 1 cut(s) 453
Psp5II RGGWCCY 1 cut(s) 929
Psp6I CCWGG 3 cut(s) 1055, 1505, 1773
PspCI CACGTG 1 cut(s) 2041
PspFI CCCAGC 1 cut(s) 1072
PspGI CCWGG 3 cut(s) 1055, 1505, 1773
PspN4I GGNNCC 5 cut(s) 104, 369, 930, 1662, 2013
PspPI GGNCC 3 cut(s) 367, 827, 929
PspPPI RGGWCCY 1 cut(s) 929
PstI CTGCAG 1 cut(s) 232
PstNI CAGNNNCTG 1 cut(s) 1333
PsuI RGATCY 2 cut(s) 247, 888
RsaI GTAC 3 cut(s) 880, 965, 1078
RsaNI GTAC 3 cut(s) 879, 964, 1077
RseI CAYNNNNRTG 2 cut(s) 1010, 2043
SacI GAGCTC 1 cut(s) 637
SalI GTCGAC 1 cut(s) 548
SatI GCNGC 2 cut(s) 2187, 2190
Sau96I GGNCC 3 cut(s) 367, 827, 929
SchI GAGTC 5 cut(s) 514, 910, 1318, 1409, 2122
ScrFI CCNGG 4 cut(s) 1057, 1507, 1775, 2089
SduI GDGCHC 2 cut(s) 526, 637
SfaNI GCATC 3 cut(s) 1132, 1510, 1858
SfcI CTRYAG 2 cut(s) 228, 1705
SfuI TTCGAA 1 cut(s) 26
SinI GGWCC 3 cut(s) 367, 827, 929
SmiMI CAYNNNNRTG 2 cut(s) 1010, 2043
SmlI CTYRAG 2 cut(s) 1565, 2268
SmoI CTYRAG 2 cut(s) 1565, 2268
SnaBI TACGTA 2 cut(s) 354, 680
SseBI AGGCCT 2 cut(s) 1356, 1936
SsiI CCGC 5 cut(s) 365, 2054, 2063, 2186, 2189
SspI AATATT 4 cut(s) 466, 662, 1459, 1546
SspMI CTAG 4 cut(s) 107, 1449, 1476, 2235
SstI GAGCTC 1 cut(s) 637
StuI AGGCCT 2 cut(s) 1356, 1936
StyD4I CCNGG 4 cut(s) 1055, 1505, 1773, 2087
TaaI ACNGT 6 cut(s) 291, 547, 1012, 1198, 1709, 1735
TaiI ACGT 4 cut(s) 356, 456, 682, 2043
TaqI TCGA 9 cut(s) 10, 26, 526, 549, 1554, 1675, 2172, 2253, 2303
TatI WGTACW 2 cut(s) 878, 963
TauI GCSGC 2 cut(s) 2189, 2192
TfiI GAWTC 6 cut(s) 138, 146, 220, 1318, 2276, 2309
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 2 cut(s) 1684, 2258
Tsp45I GTSAC 2 cut(s) 1684, 2258
TspDTI ATGAA 4 cut(s) 243, 405, 1389, 1618
TspGWI ACGGA 3 cut(s) 392, 940, 2122
TspRI CASTG 1 cut(s) 64
VpaK11BI GGWCC 3 cut(s) 367, 827, 929
XagI CCTNNNNNAGG 1 cut(s) 2171
XapI RAATTY 8 cut(s) 66, 163, 207, 307, 850, 1153, 1784, 1894
XcmI CCANNNNNNNNNTGG 1 cut(s) 1513
XmiI GTMKAC 2 cut(s) 549, 1704
XspI CTAG 4 cut(s) 107, 1449, 1476, 2235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.