Rroxscaffold_1G00040230

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000236
Physical Location & Seq
Forward (+)
57822911 .. 57825955
3045 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00040230.1

Sequence Viewer

Length: 2016 bp
ATGTGTTTAATTTCTTCAGACCATTCATTGTTTATAAATTGTGGTGGTGGAGAACCGATTAAAGTTGATGGAAATGTATATGATCAAGATAATGATACATCACAGTTTTACCTAAGTCCAAAAGGAAACTGGGCTCGAAGTAGTGCTGGAAGCACCGTTGATGCCTTCAATTCCAGTGAATTCTTAAAAAGCATGAGATGTGGGCTTTCCTCTGAAGCAACTTTATACAAGAGTGCTCGCAGTTCTCCGGTCTCTCTAAAATATTACGGGTTCTGTTTACGTAAAGGCAAATACAGGGTAATACTTCATTTTGCTGAAATTGTTTGCGAGGATATGAGGTACAAAAGTACAGATAAACGCATATTTGATGTATATATTCAGGGTGAGAGGAAACTAAAGGATTTCAACATTATAGAGAAGGCAGGAGGTCCGAATTTAGTACATGAAGAAAATTTCACAGCGGTTAATGTAAATGATGGTGTATTAGAGATACACTTCTACTCGGCTGGAAAAGGGACTCTTCAGGGACCTCTCATATCTGCTATATCCGTAACTCCAGAGTACAAGCTACACAAACAACTATCTCCTTTGCATATAGCGCTCATTACTGTGGCCTCAATCATAGCTTTCGTATTACTTTTATTGCTTTTTGCCTGGATGATGGGATGGCTGGGTACAGATCACTTACAAGAAATAGATATAGGTCTAGAAAAGCCCGTCACCCTCAAACAATTAAAAGTTGCTACAGGGAATTTTAGCAAGAGGAACGAGATTGGTCAAGGGGTTTTGGGACCGTTTACAAGGAGAAATGGAGTTAATGATGTATTTCATTTGTTTGGTTGTAATTGCGTGCAGGCTGAACTGCAAGGAAAAATTGTAGCTGTGAAGAAACTTTCCTCTCATTCAGAGGAAAGGATAAATCAGTTGAAAAATGAGTTTTATGCTTTGAAATCAATGAGTCAAGAGAACCTTGTTCAGTTACTGGACGTTTACAATGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGCAAAACAAATCCCTTGCACACGCTTTATTTGACTCAAAGTCCAAATTGAAACTTGATTGGGAAGCTAGGTTTAACATTTGCTTGGGAATAGCTAGGGGATTGGCGTATCTACATGAGCATCCCAGGCTGAAGACGGTTCACAGGGACATTAAATCAGCTAATATTCTTCTCGATGGAAACCTCAAGGCTAAAATATCAGACTTTGGATTGGCAAGCCTTTACACCGAAGATGATCAATTCAAGTTCGTCAAAGTAGAAGTGACAGAGGGATATATGGCACCCGAGTATGTTCGAGGAGTTGTGACATCTAAAGCTGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGAAAAATGCAGGACACAAGCGAGATAGCCAGGAAACTGAATTTCTTTTAGACACGGCTTGTGATTTACAGCGAAAAGGAAAGTTGGTGGACTTGGTTGATAAAACCTTGTCTAACAAGTATGATGCAAAACAAGCCATCATCATCTTGAATTTAGCAGTAAAGTGCATCAATATATCGCCAACTCTGAGGCCTACTATGTCTGAAGTTGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATCTGTTCCTCTGATCAGATTGAGGACATTAACGACCCCCATGTGAAGGAGATCGCAGAGTTCGCAGTGTCGGAGTACAACAAGAAATCTGGGAAGAAGCTGGAGTTACAGAGCGTGGTGAAGGGCGAGACTCGGGTCGTCCCCGGGGAGAATTACCGGCTCGTCATCGCCGTCAAGGATAATTCGGCTGCGGCTAAGTACGAGGGCGTTGTGTATGAGAGGATTTGGGAGCACACTAGGGAATTGCTCTCCTTCGATAGTCACATTGCACAAGTTGATTCCTCTGTTTCTATGGAAGTAACCTCGAGAGCATCCACACCATCCAATTTGATCAAAGGGGAAGATGAAACAGAACACATTTCTGAGAGTACCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

671

Amino Acids

74.95

Weight (kDa)

6.57

Isoelectric Point (pI)

40.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 9 - 181 6.5e-31 Malectin domain
PK_Tyr_Ser-Thr PF07714 286 - 537 7.4e-38 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 287 - 535 1.2e-41 Protein kinase domain
SQAPI PF16845 553 - 632 3.8e-30 Aspartic acid proteinase inhibitor
Cystatin PF00031 553 - 610 6.9e-12 Cystatin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 35
AccB1I GGYRCC 1 cut(s) 1309
AccI GTMKAC 1 cut(s) 1353
AciI CCGC 2 cut(s) 461, 1829
AcsI RAATTY 6 cut(s) 179, 433, 451, 751, 1433, 1543
AcuI CTGAAG 4 cut(s) 234, 506, 1181, 1617
AfaI GTAC 8 cut(s) 341, 349, 441, 563, 676, 1715, 1838, 2008
AfeI AGCGCT 1 cut(s) 600
AfiI CCNNNNNNNGG 1 cut(s) 1684
AgsI TTSAA 8 cut(s) 169, 406, 928, 949, 1081, 1273, 1379, 1543
AhdI GACNNNNNGTC 1 cut(s) 1069
AjnI CCWGG 3 cut(s) 653, 1154, 1422
AluBI AGCT 8 cut(s) 568, 626, 881, 1097, 1124, 1190, 1346, 1738
AluI AGCT 8 cut(s) 568, 626, 881, 1097, 1124, 1190, 1346, 1738
Alw21I GWGCWC 2 cut(s) 238, 1872
Alw26I GTCTC 2 cut(s) 256, 1760
AlwNI CAGNNNCTG 1 cut(s) 982
Ama87I CYCGRG 4 cut(s) 1313, 1770, 1781, 1942
Aor51HI AGCGCT 1 cut(s) 600
AoxI GGCC 3 cut(s) 612, 1003, 1583
ApeKI GCWGC 1 cut(s) 1826
ApoI RAATTY 6 cut(s) 179, 433, 451, 751, 1433, 1543
AspLEI GCGC 1 cut(s) 601
AspS9I GGNCC 3 cut(s) 428, 527, 791
AsuC2I CCSGG 2 cut(s) 1782, 1783
AsuHPI GGTGA 3 cut(s) 395, 712, 1768
AvaI CYCGRG 4 cut(s) 1313, 1770, 1781, 1942
AvaII GGWCC 3 cut(s) 428, 527, 791
BaeI ACNNNNGTAYC 2 cut(s) 331, 364
BanI GGYRCC 1 cut(s) 1309
BanII GRGCYC 1 cut(s) 136
BbsI GAAGAC 1 cut(s) 1169
Bbv12I GWGCWC 2 cut(s) 238, 1872
BbvI GCAGC 1 cut(s) 1813
BccI CCATC 7 cut(s) 62, 470, 655, 660, 1199, 1538, 1966
BceAI ACGGC 2 cut(s) 1464, 1793
BciT130I CCWGG 3 cut(s) 655, 1156, 1424
BclI TGATCA 4 cut(s) 82, 1264, 1651, 1968
BcnI CCSGG 2 cut(s) 1782, 1783
BcoDI GTCTC 2 cut(s) 256, 1760
BfaI CTAG 5 cut(s) 707, 1098, 1125, 1875, 2014
BfmI CTRYAG 2 cut(s) 744, 1354
BfoI RGCGCY 1 cut(s) 602
BglII AGATCT 1 cut(s) 1638
BisI GCNGC 2 cut(s) 1827, 1830
BlsI GCNGC 2 cut(s) 1828, 1831
Bme1390I CCNGG 5 cut(s) 655, 1156, 1424, 1782, 1783
Bme18I GGWCC 3 cut(s) 428, 527, 791
BmeRI GACNNNNNGTC 1 cut(s) 1069
BmeT110I CYCGRG 4 cut(s) 1313, 1770, 1781, 1942
BmgT120I GGNCC 3 cut(s) 428, 527, 791
BmiI GGNNCC 3 cut(s) 528, 792, 1311
BmrFI CCNGG 5 cut(s) 655, 1156, 1424, 1782, 1783
BmrI ACTGGG 1 cut(s) 139
BmsI GCATC 5 cut(s) 151, 1159, 1507, 1569, 1958
BmuI ACTGGG 1 cut(s) 139
BoxI GACNNNNGTC 1 cut(s) 1772
BpiI GAAGAC 1 cut(s) 1169
BpmI CTGGAG 2 cut(s) 540, 1760
BpuEI CTTGAG 1 cut(s) 1199
BpuMI CCSGG 2 cut(s) 1782, 1783
BsaAI YACGTR 1 cut(s) 281
BsaI GGTCTC 1 cut(s) 256
BsaJI CCNNGG 4 cut(s) 1154, 1780, 1781, 1782
BsaWI WCCGGW 1 cut(s) 247
Bsc4I CCNNNNNNNGG 1 cut(s) 1684
Bse118I RCCGGY 1 cut(s) 1794
Bse1I ACTGG 3 cut(s) 134, 174, 987
Bse3DI GCAATG 1 cut(s) 1902
BseBI CCWGG 3 cut(s) 655, 1156, 1424
BseDI CCNNGG 4 cut(s) 1154, 1780, 1781, 1782
BseGI GGATG 5 cut(s) 663, 671, 1150, 1949, 1958
BseLI CCNNNNNNNGG 1 cut(s) 1684
BseMI GCAATG 1 cut(s) 1902
BseMII CTCAG 2 cut(s) 1571, 1992
BseNI ACTGG 3 cut(s) 134, 174, 987
BseRI GAGGAG 2 cut(s) 1341, 1649
BseXI GCAGC 1 cut(s) 1813
BseYI CCCAGC 1 cut(s) 670
BsgI GTGCAG 1 cut(s) 872
BshFI GGCC 3 cut(s) 614, 1005, 1585
BshNI GGYRCC 1 cut(s) 1309
BsiHKAI GWGCWC 2 cut(s) 238, 1872
BsiHKCI CYCGRG 4 cut(s) 1313, 1770, 1781, 1942
BsiSI CCGG 3 cut(s) 248, 1782, 1795
BslFI GGGAC 5 cut(s) 529, 540, 804, 1190, 1763
BslI CCNNNNNNNGG 1 cut(s) 1684
BsmAI GTCTC 2 cut(s) 256, 1760
BsmFI GGGAC 5 cut(s) 529, 540, 804, 1190, 1763
BsnI GGCC 3 cut(s) 614, 1005, 1585
Bso31I GGTCTC 1 cut(s) 256
BsoBI CYCGRG 4 cut(s) 1313, 1770, 1781, 1942
Bsp1286I GDGCHC 3 cut(s) 136, 238, 1872
Bsp143I GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
BspACI CCGC 2 cut(s) 461, 1829
BspANI GGCC 3 cut(s) 614, 1005, 1585
BspCNI CTCAG 2 cut(s) 1572, 1993
BspLI GGNNCC 3 cut(s) 528, 792, 1311
BspT107I GGYRCC 1 cut(s) 1309
BspTNI GGTCTC 1 cut(s) 256
BsrDI GCAATG 1 cut(s) 1902
BsrFI RCCGGY 1 cut(s) 1794
BsrI ACTGG 3 cut(s) 134, 174, 987
BssAI RCCGGY 1 cut(s) 1794
BssECI CCNNGG 4 cut(s) 1154, 1780, 1781, 1782
BssMI GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
Bst2UI CCWGG 3 cut(s) 655, 1156, 1424
Bst4CI ACNGT 7 cut(s) 105, 157, 610, 795, 1168, 1358, 1384
Bst6I CTCTTC 1 cut(s) 525
BstBAI YACGTR 1 cut(s) 281
BstC8I GCNNGC 5 cut(s) 238, 851, 855, 1007, 1246
BstDEI CTNAG 4 cut(s) 113, 1580, 1833, 2001
BstF5I GGATG 5 cut(s) 663, 671, 1150, 1949, 1958
BstH2I RGCGCY 1 cut(s) 602
BstHHI GCGC 1 cut(s) 601
BstKTI GATC 7 cut(s) 85, 682, 1267, 1641, 1654, 1692, 1971
BstMAI GTCTC 2 cut(s) 256, 1760
BstMBI GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
BstMWI GCNNNNNNNGC 4 cut(s) 598, 1156, 1526, 1700
BstNI CCWGG 3 cut(s) 655, 1156, 1424
BstPAI GACNNNNGTC 1 cut(s) 1772
BstSCI CCNGG 5 cut(s) 653, 1154, 1422, 1780, 1781
BstSFI CTRYAG 2 cut(s) 744, 1354
BstSNI TACGTA 1 cut(s) 281
BstV1I GCAGC 1 cut(s) 1813
BstV2I GAAGAC 1 cut(s) 1169
BstX2I RGATCY 1 cut(s) 1638
BstYI RGATCY 1 cut(s) 1638
BsuRI GGCC 3 cut(s) 614, 1005, 1585
BtgZI GCGATG 1 cut(s) 1789
BtsCI GGATG 5 cut(s) 663, 671, 1150, 1949, 1958
BtsI GCAGTG 1 cut(s) 1710
BtsIMutI CAGTG 2 cut(s) 181, 1710
Cac8I GCNNGC 5 cut(s) 238, 851, 855, 1007, 1246
CaiI CAGNNNCTG 1 cut(s) 982
CfoI GCGC 1 cut(s) 601
Cfr10I RCCGGY 1 cut(s) 1794
Cfr13I GGNCC 3 cut(s) 428, 527, 791
Cfr9I CCCGGG 1 cut(s) 1781
Csp6I GTAC 8 cut(s) 340, 348, 440, 562, 675, 1714, 1837, 2007
CviAII CATG 4 cut(s) 193, 443, 1145, 1679
CviQI GTAC 8 cut(s) 340, 348, 440, 562, 675, 1714, 1837, 2007
DdeI CTNAG 4 cut(s) 113, 1580, 1833, 2001
DpnI GATC 7 cut(s) 84, 681, 1266, 1640, 1653, 1691, 1970
DpnII GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
DriI GACNNNNNGTC 1 cut(s) 1069
Eam1104I CTCTTC 1 cut(s) 525
Eam1105I GACNNNNNGTC 1 cut(s) 1069
EarI CTCTTC 1 cut(s) 525
Eco105I TACGTA 1 cut(s) 281
Eco147I AGGCCT 2 cut(s) 1005, 1585
Eco24I GRGCYC 1 cut(s) 136
Eco31I GGTCTC 1 cut(s) 256
Eco47I GGWCC 3 cut(s) 428, 527, 791
Eco47III AGCGCT 1 cut(s) 600
Eco57I CTGAAG 4 cut(s) 234, 506, 1181, 1617
Eco88I CYCGRG 4 cut(s) 1313, 1770, 1781, 1942
EcoO109I RGGNCCY 1 cut(s) 527
EcoRI GAATTC 1 cut(s) 179
EcoRII CCWGG 3 cut(s) 653, 1154, 1422
EcoT38I GRGCYC 1 cut(s) 136
FaeI CATG 4 cut(s) 196, 446, 1148, 1682
FalI AAGNNNNNCTT 4 cut(s) 504, 536, 954, 986
FaqI GGGAC 5 cut(s) 529, 540, 804, 1190, 1763
FatI CATG 4 cut(s) 192, 442, 1144, 1678
FbaI TGATCA 4 cut(s) 82, 1264, 1651, 1968
FblI GTMKAC 1 cut(s) 1353
Fnu4HI GCNGC 2 cut(s) 1827, 1830
FokI GGATG 5 cut(s) 670, 678, 1137, 1936, 1945
FriOI GRGCYC 1 cut(s) 136
Fsp4HI GCNGC 2 cut(s) 1827, 1830
FspBI CTAG 5 cut(s) 707, 1098, 1125, 1875, 2014
GlaI GCGC 1 cut(s) 600
GluI GCNGC 2 cut(s) 1827, 1830
GsaI CCCAGC 1 cut(s) 674
GsuI CTGGAG 2 cut(s) 540, 1760
HaeII RGCGCY 1 cut(s) 602
HaeIII GGCC 3 cut(s) 614, 1005, 1585
HapII CCGG 3 cut(s) 248, 1782, 1795
HhaI GCGC 1 cut(s) 601
Hin1II CATG 4 cut(s) 196, 446, 1148, 1682
Hin6I GCGC 1 cut(s) 599
HinP1I GCGC 1 cut(s) 599
HinfI GANTC 5 cut(s) 517, 958, 1064, 1768, 1916
HpaII CCGG 3 cut(s) 248, 1782, 1795
HphI GGTGA 3 cut(s) 395, 712, 1768
Hpy166II GTNNAC 6 cut(s) 278, 798, 991, 1171, 1354, 1483
Hpy188III TCNNGA 7 cut(s) 86, 557, 707, 962, 1202, 1540, 1944
Hpy8I GTNNAC 6 cut(s) 278, 798, 991, 1171, 1354, 1483
HpyAV CCTTC 6 cut(s) 175, 412, 1386, 1678, 1753, 1900
HpyCH4III ACNGT 7 cut(s) 105, 157, 610, 795, 1168, 1358, 1384
HpyCH4IV ACGT 2 cut(s) 280, 987
HpyF10VI GCNNNNNNNGC 4 cut(s) 598, 1156, 1526, 1700
HpyF3I CTNAG 4 cut(s) 113, 1580, 1833, 2001
HpySE526I ACGT 2 cut(s) 280, 987
Hsp92II CATG 4 cut(s) 196, 446, 1148, 1682
HspAI GCGC 1 cut(s) 599
Ksp22I TGATCA 4 cut(s) 82, 1264, 1651, 1968
Kzo9I GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
LmnI GCTCC 1 cut(s) 1867
Lsp1109I GCAGC 1 cut(s) 1813
LweI GCATC 5 cut(s) 151, 1159, 1507, 1569, 1958
MaeI CTAG 5 cut(s) 707, 1098, 1125, 1875, 2014
MaeII ACGT 2 cut(s) 280, 987
MaeIII GTNAC 8 cut(s) 550, 718, 978, 1291, 1333, 1743, 1898, 1936
MalI GATC 7 cut(s) 84, 681, 1266, 1640, 1653, 1691, 1970
MboI GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
MboII GAAGA 9 cut(s) 6, 458, 512, 898, 1174, 1190, 1271, 1744, 1991
MflI RGATCY 1 cut(s) 1638
MhlI GDGCHC 3 cut(s) 136, 238, 1872
MlyI GAGTC 4 cut(s) 511, 967, 1058, 1762
MmeI TCCRAC 1 cut(s) 1689
MseI TTAA 9 cut(s) 8, 60, 185, 465, 734, 816, 1104, 1182, 1668
MslI CAYNNNNRTG 1 cut(s) 608
MspA1I CMGCKG 1 cut(s) 461
MspI CCGG 3 cut(s) 248, 1782, 1795
MspR9I CCNGG 5 cut(s) 655, 1156, 1424, 1782, 1783
MvaI CCWGG 3 cut(s) 655, 1156, 1424
MwoI GCNNNNNNNGC 4 cut(s) 598, 1156, 1526, 1700
NciI CCSGG 2 cut(s) 1782, 1783
NdeII GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
NlaIII CATG 4 cut(s) 196, 446, 1148, 1682
NlaIV GGNNCC 3 cut(s) 528, 792, 1311
NmeAIII GCCGAG 1 cut(s) 482
NmuCI GTSAC 4 cut(s) 718, 1291, 1333, 1898
PaeR7I CTCGAG 1 cut(s) 1942
PceI AGGCCT 2 cut(s) 1005, 1585
PcsI WCGNNNNNNNCGW 1 cut(s) 1806
PfeI GAWTC 1 cut(s) 1916
PkrI GCNGC 2 cut(s) 1828, 1831
PleI GAGTC 4 cut(s) 511, 966, 1058, 1762
PpsI GAGTC 4 cut(s) 511, 966, 1058, 1762
Ppu21I YACGTR 1 cut(s) 281
PpuMI RGGWCCY 1 cut(s) 527
PshAI GACNNNNGTC 1 cut(s) 1772
PsiI TTATAA 1 cut(s) 35
Psp5II RGGWCCY 1 cut(s) 527
Psp6I CCWGG 3 cut(s) 653, 1154, 1422
PspFI CCCAGC 1 cut(s) 670
PspGI CCWGG 3 cut(s) 653, 1154, 1422
PspN4I GGNNCC 3 cut(s) 528, 792, 1311
PspPI GGNCC 3 cut(s) 428, 527, 791
PspPPI RGGWCCY 1 cut(s) 527
PstNI CAGNNNCTG 1 cut(s) 982
PsuI RGATCY 1 cut(s) 1638
RsaI GTAC 8 cut(s) 341, 349, 441, 563, 676, 1715, 1838, 2008
RsaNI GTAC 8 cut(s) 340, 348, 440, 562, 675, 1714, 1837, 2007
RseI CAYNNNNRTG 1 cut(s) 608
SaqAI TTAA 9 cut(s) 8, 60, 185, 465, 734, 816, 1104, 1182, 1668
SatI GCNGC 2 cut(s) 1827, 1830
Sau3AI GATC 7 cut(s) 82, 679, 1264, 1638, 1651, 1689, 1968
Sau96I GGNCC 3 cut(s) 428, 527, 791
SchI GAGTC 4 cut(s) 511, 967, 1058, 1762
ScrFI CCNGG 5 cut(s) 655, 1156, 1424, 1782, 1783
SduI GDGCHC 3 cut(s) 136, 238, 1872
SfaNI GCATC 5 cut(s) 151, 1159, 1507, 1569, 1958
SfcI CTRYAG 2 cut(s) 744, 1354
Sfr274I CTCGAG 1 cut(s) 1942
SinI GGWCC 3 cut(s) 428, 527, 791
SlaI CTCGAG 1 cut(s) 1942
SmaI CCCGGG 1 cut(s) 1783
SmiMI CAYNNNNRTG 1 cut(s) 608
SmlI CTYRAG 2 cut(s) 1214, 1942
SmoI CTYRAG 2 cut(s) 1214, 1942
SnaBI TACGTA 1 cut(s) 281
SseBI AGGCCT 2 cut(s) 1005, 1585
SsiI CCGC 2 cut(s) 461, 1829
SspI AATATT 2 cut(s) 263, 1195
SspMI CTAG 5 cut(s) 707, 1098, 1125, 1875, 2014
StuI AGGCCT 2 cut(s) 1005, 1585
StyD4I CCNGG 5 cut(s) 653, 1154, 1422, 1780, 1781
TaaI ACNGT 7 cut(s) 105, 157, 610, 795, 1168, 1358, 1384
TaiI ACGT 2 cut(s) 283, 990
TaqI TCGA 5 cut(s) 136, 1203, 1324, 1893, 1943
TatI WGTACW 4 cut(s) 347, 439, 561, 1713
TauI GCSGC 1 cut(s) 1832
TfiI GAWTC 1 cut(s) 1916
Tru1I TTAA 9 cut(s) 8, 60, 185, 465, 734, 816, 1104, 1182, 1668
Tru9I TTAA 9 cut(s) 8, 60, 185, 465, 734, 816, 1104, 1182, 1668
TscAI CASTG 2 cut(s) 181, 1710
TseFI GTSAC 4 cut(s) 718, 1291, 1333, 1898
TseI GCWGC 1 cut(s) 1826
Tsp45I GTSAC 4 cut(s) 718, 1291, 1333, 1898
TspDTI ATGAA 6 cut(s) 15, 296, 459, 818, 1038, 1998
TspGWI ACGGA 1 cut(s) 538
TspMI CCCGGG 1 cut(s) 1781
TspRI CASTG 2 cut(s) 181, 1710
VpaK11BI GGWCC 3 cut(s) 428, 527, 791
XapI RAATTY 6 cut(s) 179, 433, 451, 751, 1433, 1543
XbaI TCTAGA 1 cut(s) 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 126
XhoI CTCGAG 1 cut(s) 1942
XmaI CCCGGG 1 cut(s) 1781
XmiI GTMKAC 1 cut(s) 1353
XspI CTAG 5 cut(s) 707, 1098, 1125, 1875, 2014
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.