Rh4BG032700

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000236
Physical Location & Seq
Reverse (-)
5868548 .. 5999734
131187 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG032700.1

Sequence Viewer

Length: 2394 bp
ATGGGGACCGAAGCTTCAGCGAGACTTGTCAACTTTACTATTATAAGTATATGCCTCTTCTCTACTCTTTCTCAGCTTGGTTCTGAATTCAAATGTCAGAGTATAGCTCAAAAGTTGCCCGATGAAGAAGTGAGAGCTCTTAATCAAATAATGGAGAAGTTGGAGTTCAACAATATTAATCGAGTGCCCTCTAAATCATTTTGCAAAAAGAGCGGTCGTGATGGCATCAGATGTAACTGCAGTTTTCAAAACGGCACCATTTGCCATGTCACAGAAATGGATCTTAGTAGGAATCAATTGACTGATTCTATACCTGCTAGCTTGGGGACTTTATCTTCGTTGTACAAGCTGATTCTTTACAGCAATCAGTTGACCGGGCCCATTCCAGAGGGTTTGGGAAATATGAAATTTCAAGTCCGCATGGGTCATACTTCGCACCGCAGCATGGCTGTTAGCTTCGAACTGGATCTTTCATACAATCAATTAAGTGGATCCATACCAGCCACTTTAAGGAATGTGGATGTCTCTGATGTGGCATCTGATCCAGATGCCTACCGACTCTTCAAGCTTAAGCAATACGACAGGGCTAATGTTGACCCACAGTTCTACCTGGATCTTTCAAGCAATCAACTGACTGGGCCTATTCCAGAGAGCTTAGGGAATTTGACTTTCATAAGTTTTATTGAACTGTATAACAACTTTCTCAACGGCTCTATACCAGCATCTTTGGGTGCACTGACTTATCTGCATGTTCTGTCTATGGAAAACAATAGAATCTCCGGCAGTCTCCCACAAACACTAGGAAATTTGACAATCCTTAATTACCTCGGGGTCGCATCTAACAGTATTAGTGGGACAGTACCAAAAAGTTTTGCCAGCCTTACGAGTCTGTATGCATTTTATTTATCTGGTAACTATTTGTCTGGCCCGTTTCCTGACTTCATAGCAGCCAACTGGACTTTAATCGAAGAGATTTTTCTCAACGGAAACAATTTCAAGGGGAAGATACCTGCCGGAATTTTTAATTTATCACATCTAGAAATGTTGTCAATAAGTGATGTTGAAGCTGACTCTCATTTCCAGTTCCCACCATCTATACACGTGGCGAAAAGTTTTAGTGTATTGATACTAAGAAACTGCTCAATCACTGGTCAAATTCCTTCTTACATAGGAAATATGTTATCATTAACGAACATAGACTTGAGCTTCAACAGGTTAACGGGTAGAATCCCAGATTCTTTGAAAAACTTAAATGTAACTCACATGTCTTTTTCAAAAAATATGCTTACTGGGAAAATTCCTGATTGGATTCAAAATGCAGTATGGAGCAAGATGGATTTTTCATACAATAATTTTTCAAAGCTAACCTTTAAACCGTCACCCAATCTACAACTGAATTTGTTTTCCTCCTGCTGCAACTCCTCAACTTGTCTGCCAAATTCGACAGACACAATCAAGGAGAAGTCTTGTCCCCGAAAACCTAAGTATCATTCATTGTTCATAAATTGTGGCGGTGGGGAAACACGTGGTGCTGATGGAAATCTTTATGATCAAGATAATGATACGTTACTATATTACCAGAGTCCAAAAGGAAACTGGGCTCGGAGTAGTGTTGGAAACAGCTACGACTATGCTTCCGATGGCAATTCTAGTAAATTGTTAAAAAACGTTAGATGTGGGCTTTCATCTGAAGCACGTTTATACGATACAGCTCGCATTTCCTTTGTCTCTTTAAAATATTACGGGTTCTGTTTACGTAAAGGCAAATACCGTGTAGCACTTCACTTTGCTGAGATTGTTGATGAAGTTACAGATATTCTTGAGGATATTGATTTTAGAAGTACAGATAAACGCACATTTGATGTATATATTCAGGCTGAAGTGCAAGGGAAAATTGTAGCCGTGAAGAAACTTCGCTCTCATTGCCAGGAAAGGATCAATGAGTTCATAAATGAATTTTATACCTTAAAAGCAATGAGTCAAGAGAACCTTGTTCAGTTGCTGGACATTTACAACGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGGAGAACAACTCCCTTGCACATGCCTTATTTGGAAGGTTGGTGGACTTGGTTGACAAAACCTTGTCCAACAAGTATGATGCAAAACAAGCCATAATCATTTTGAATTTAGCAGTAAAGTGCACCAATATATCTCCAACTCTGAGGCCTACTATGTCTGAAGTAGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTACTCCTGCTCTTAGTGATAGTCACCTTGCTCAAGTTGATTCCTCTGTTTCTATGGAAGCAACCTCGAGAGCATCCACATCATCCAATTTGATCAAAGGGGAAGAGGAAACAGAACACATTTATGAGAGTACCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

797

Amino Acids

88.66

Weight (kDa)

6.34

Isoelectric Point (pI)

33.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 211 - 297 1.6e-06 Leucine-rich repeat region
LRR_8 PF13855 248 - 307 6.2e-07 Leucine rich repeat
Malectin PF11721 498 - 628 3e-15 Malectin domain
PK_Tyr_Ser-Thr PF07714 626 - 694 6.9e-08 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 628 - 693 9.5e-07 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
Acc36I ACCTGC 2 cut(s) 322, 1018
AccB1I GGYRCC 1 cut(s) 254
AccBSI CCGCTC 1 cut(s) 213
AciI CCGC 4 cut(s) 213, 418, 439, 1512
AclI AACGTT 1 cut(s) 1668
AclWI GGATC 7 cut(s) 288, 474, 486, 499, 536, 621, 1943
AcuI CTGAAG 3 cut(s) 1710, 1899, 2229
AcvI CACGTG 2 cut(s) 1102, 1526
AdeI CACNNNGTG 1 cut(s) 1529
AfaI GTAC 5 cut(s) 344, 861, 1843, 2257, 2386
AfiI CCNNNNNNNGG 2 cut(s) 445, 510
AflII CTTAAG 1 cut(s) 569
AflIII ACRYGT 3 cut(s) 1099, 1263, 1523
AjnI CCWGG 2 cut(s) 609, 1926
Alw21I GWGCWC 3 cut(s) 139, 736, 2174
Alw26I GTCTC 4 cut(s) 16, 529, 791, 1732
Alw44I GTGCAC 2 cut(s) 732, 2170
AlwI GGATC 7 cut(s) 288, 474, 486, 499, 536, 621, 1943
AlwNI CAGNNNCTG 1 cut(s) 2002
Ama87I CYCGRG 2 cut(s) 827, 2320
AoxI GGCC 5 cut(s) 377, 638, 925, 2023, 2195
ApaI GGGCCC 1 cut(s) 381
ApaLI GTGCAC 2 cut(s) 732, 2170
ApeKI GCWGC 3 cut(s) 441, 947, 1413
ArsI GACNNNNNNTTYG 2 cut(s) 319, 351
AseI ATTAAT 1 cut(s) 177
AspS9I GGNCC 5 cut(s) 6, 377, 378, 638, 926
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 1371, 2270
AsuII TTCGAA 1 cut(s) 459
AsuNHI GCTAGC 1 cut(s) 317
AvaI CYCGRG 2 cut(s) 827, 2320
AvaII GGWCC 1 cut(s) 6
BaeGI GKGCMC 4 cut(s) 189, 381, 736, 2174
BaeI ACNNNNGTAYC 2 cut(s) 1469, 1502
BamHI GGATCC 1 cut(s) 491
BanI GGYRCC 1 cut(s) 254
BanII GRGCYC 3 cut(s) 139, 381, 1603
BbrPI CACGTG 2 cut(s) 1102, 1526
Bbv12I GWGCWC 3 cut(s) 139, 736, 2174
BbvI GCAGC 3 cut(s) 453, 959, 1400
BccI CCATC 5 cut(s) 215, 1099, 1327, 1529, 1634
BceAI ACGGC 3 cut(s) 268, 724, 1886
BciT130I CCWGG 2 cut(s) 611, 1928
BclI TGATCA 2 cut(s) 1549, 2346
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 4 cut(s) 16, 529, 791, 1732
BfaI CTAG 5 cut(s) 318, 800, 1037, 1650, 2392
BfmI CTRYAG 1 cut(s) 238
BfrI CTTAAG 1 cut(s) 569
BfuAI ACCTGC 2 cut(s) 322, 1018
BisI GCNGC 3 cut(s) 442, 948, 1414
BlsI GCNGC 3 cut(s) 443, 949, 1415
Bme1390I CCNGG 3 cut(s) 376, 611, 1928
Bme18I GGWCC 1 cut(s) 6
BmeT110I CYCGRG 2 cut(s) 827, 2320
BmgT120I GGNCC 5 cut(s) 6, 377, 378, 638, 926
BmiI GGNNCC 4 cut(s) 7, 256, 379, 493
BmrFI CCNGG 3 cut(s) 376, 611, 1928
BmrI ACTGGG 3 cut(s) 645, 1299, 1606
BmsI GCATC 7 cut(s) 234, 538, 545, 731, 845, 2119, 2336
BmtI GCTAGC 1 cut(s) 321
BmuI ACTGGG 3 cut(s) 645, 1299, 1606
BplI GAGNNNNNCTC 4 cut(s) 91, 123, 2045, 2077
Bpu10I CCTNAGC 1 cut(s) 655
Bpu14I TTCGAA 1 cut(s) 459
BpuEI CTTGAG 3 cut(s) 1222, 1841, 2271
BpuMI CCSGG 1 cut(s) 376
BsaAI YACGTR 3 cut(s) 1102, 1526, 1757
BsaBI GATNNNNATC 1 cut(s) 1539
BsaJI CCNNGG 1 cut(s) 826
Bsc4I CCNNNNNNNGG 2 cut(s) 445, 510
Bse1I ACTGG 7 cut(s) 468, 640, 959, 1081, 1153, 1294, 1601
Bse3DI GCAATG 2 cut(s) 1921, 1980
Bse8I GATNNNNATC 1 cut(s) 1539
BseBI CCWGG 2 cut(s) 611, 1928
BseDI CCNNGG 1 cut(s) 826
BseGI GGATG 3 cut(s) 526, 2327, 2336
BseJI GATNNNNATC 1 cut(s) 1539
BseLI CCNNNNNNNGG 2 cut(s) 445, 510
BseMI GCAATG 2 cut(s) 1921, 1980
BseMII CTCAG 3 cut(s) 86, 1782, 2183
BseNI ACTGG 7 cut(s) 468, 640, 959, 1081, 1153, 1294, 1601
BseRI GAGGAG 2 cut(s) 1411, 2261
BseSI GKGCMC 4 cut(s) 189, 381, 736, 2174
BseXI GCAGC 3 cut(s) 453, 959, 1400
Bsh1285I CGRYCG 1 cut(s) 217
BshFI GGCC 5 cut(s) 379, 640, 927, 2025, 2197
BshNI GGYRCC 1 cut(s) 254
BsiEI CGRYCG 1 cut(s) 217
BsiHKAI GWGCWC 3 cut(s) 139, 736, 2174
BsiHKCI CYCGRG 2 cut(s) 827, 2320
BsiSI CCGG 3 cut(s) 375, 780, 1014
BslFI GGGAC 4 cut(s) 19, 340, 868, 1455
BslI CCNNNNNNNGG 2 cut(s) 445, 510
BsmAI GTCTC 4 cut(s) 16, 529, 791, 1732
BsmFI GGGAC 4 cut(s) 19, 340, 868, 1455
BsnI GGCC 5 cut(s) 379, 640, 927, 2025, 2197
BsoBI CYCGRG 2 cut(s) 827, 2320
Bsp119I TTCGAA 1 cut(s) 459
Bsp120I GGGCCC 1 cut(s) 377
Bsp1286I GDGCHC 6 cut(s) 139, 189, 381, 736, 1603, 2174
Bsp1407I TGTACA 1 cut(s) 342
Bsp143I GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
BspACI CCGC 4 cut(s) 213, 418, 439, 1512
BspANI GGCC 5 cut(s) 379, 640, 927, 2025, 2197
BspCNI CTCAG 3 cut(s) 85, 1783, 2184
BspLI GGNNCC 4 cut(s) 7, 256, 379, 493
BspMAI CTGCAG 1 cut(s) 242
BspMI ACCTGC 2 cut(s) 322, 1018
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 7 cut(s) 288, 474, 486, 499, 536, 621, 1943
BspT104I TTCGAA 1 cut(s) 459
BspT107I GGYRCC 1 cut(s) 254
BspTI CTTAAG 1 cut(s) 569
BsrBI CCGCTC 1 cut(s) 213
BsrDI GCAATG 2 cut(s) 1921, 1980
BsrGI TGTACA 1 cut(s) 342
BsrI ACTGG 7 cut(s) 468, 640, 959, 1081, 1153, 1294, 1601
BssECI CCNNGG 1 cut(s) 826
BssMI GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
Bst2UI CCWGG 2 cut(s) 611, 1928
Bst4CI ACNGT 6 cut(s) 603, 690, 845, 859, 1377, 1772
Bst6I CTCTTC 4 cut(s) 62, 566, 963, 2352
BstAFI CTTAAG 1 cut(s) 569
BstAPI GCANNNNNTGC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 342
BstBAI YACGTR 3 cut(s) 1102, 1526, 1757
BstBI TTCGAA 1 cut(s) 459
BstC8I GCNNGC 4 cut(s) 319, 877, 1714, 2027
BstDEI CTNAG 8 cut(s) 72, 284, 655, 1130, 1482, 1791, 2192, 2267
BstF5I GGATG 3 cut(s) 526, 2327, 2336
BstKTI GATC 8 cut(s) 283, 469, 494, 544, 616, 1552, 1938, 2349
BstMAI GTCTC 4 cut(s) 16, 529, 791, 1732
BstMBI GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
BstMCI CGRYCG 1 cut(s) 217
BstMWI GCNNNNNNNGC 4 cut(s) 210, 261, 1923, 2138
BstNI CCWGG 2 cut(s) 611, 1928
BstNSI RCATGY 3 cut(s) 752, 1267, 2075
BstSCI CCNGG 3 cut(s) 374, 609, 1926
BstSFI CTRYAG 1 cut(s) 238
BstSLI GKGCMC 4 cut(s) 189, 381, 736, 2174
BstSNI TACGTA 1 cut(s) 1757
BstV1I GCAGC 3 cut(s) 453, 959, 1400
BstX2I RGATCY 4 cut(s) 280, 466, 491, 613
BstYI RGATCY 4 cut(s) 280, 466, 491, 613
BsuRI GGCC 5 cut(s) 379, 640, 927, 2025, 2197
BtsCI GGATG 3 cut(s) 526, 2327, 2336
BtsIMutI CAGTG 2 cut(s) 734, 1146
BveI ACCTGC 2 cut(s) 322, 1018
Cac8I GCNNGC 4 cut(s) 319, 877, 1714, 2027
CaiI CAGNNNCTG 1 cut(s) 2002
Cfr13I GGNCC 5 cut(s) 6, 377, 378, 638, 926
Csp6I GTAC 5 cut(s) 343, 860, 1842, 2256, 2385
CviAII CATG 6 cut(s) 266, 421, 445, 749, 1264, 2072
CviQI GTAC 5 cut(s) 343, 860, 1842, 2256, 2385
DdeI CTNAG 8 cut(s) 72, 284, 655, 1130, 1482, 1791, 2192, 2267
DpnI GATC 8 cut(s) 282, 468, 493, 543, 615, 1551, 1937, 2348
DpnII GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
DraI TTTAAA 2 cut(s) 1372, 1734
DraIII CACNNNGTG 1 cut(s) 1529
Eam1104I CTCTTC 4 cut(s) 62, 566, 963, 2352
EarI CTCTTC 4 cut(s) 62, 566, 963, 2352
Ecl136II GAGCTC 1 cut(s) 137
Eco105I TACGTA 1 cut(s) 1757
Eco147I AGGCCT 2 cut(s) 2025, 2197
Eco24I GRGCYC 3 cut(s) 139, 381, 1603
Eco47I GGWCC 1 cut(s) 6
Eco53kI GAGCTC 1 cut(s) 137
Eco57I CTGAAG 3 cut(s) 1710, 1899, 2229
Eco72I CACGTG 2 cut(s) 1102, 1526
Eco88I CYCGRG 2 cut(s) 827, 2320
EcoICRI GAGCTC 1 cut(s) 137
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 2 cut(s) 609, 1926
EcoT22I ATGCAT 1 cut(s) 898
EcoT38I GRGCYC 3 cut(s) 139, 381, 1603
FaeI CATG 6 cut(s) 269, 424, 448, 752, 1267, 2075
FalI AAGNNNNNCTT 4 cut(s) 1352, 1384, 1974, 2006
FaqI GGGAC 4 cut(s) 19, 340, 868, 1455
FatI CATG 6 cut(s) 265, 420, 444, 748, 1263, 2071
FbaI TGATCA 2 cut(s) 1549, 2346
Fnu4HI GCNGC 3 cut(s) 442, 948, 1414
FokI GGATG 3 cut(s) 533, 2314, 2323
FriOI GRGCYC 3 cut(s) 139, 381, 1603
Fsp4HI GCNGC 3 cut(s) 442, 948, 1414
FspBI CTAG 5 cut(s) 318, 800, 1037, 1650, 2392
GluI GCNGC 3 cut(s) 442, 948, 1414
HaeIII GGCC 5 cut(s) 379, 640, 927, 2025, 2197
HapII CCGG 3 cut(s) 375, 780, 1014
Hin1II CATG 6 cut(s) 269, 424, 448, 752, 1267, 2075
HincII GTYRAC 5 cut(s) 31, 372, 595, 1218, 2104
HindII GTYRAC 5 cut(s) 31, 372, 595, 1218, 2104
HindIII AAGCTT 2 cut(s) 12, 566
HpaI GTTAAC 1 cut(s) 1218
HpaII CCGG 3 cut(s) 375, 780, 1014
HphI GGTGA 2 cut(s) 1371, 2270
Hpy166II GTNNAC 9 cut(s) 31, 372, 595, 734, 1218, 1754, 2095, 2104, 2172
Hpy8I GTNNAC 9 cut(s) 31, 372, 595, 734, 1218, 1754, 2095, 2104, 2172
HpyAV CCTTC 2 cut(s) 1170, 2079
HpyCH4III ACNGT 6 cut(s) 603, 690, 845, 859, 1377, 1772
HpyCH4IV ACGT 6 cut(s) 1101, 1525, 1565, 1668, 1696, 1756
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 261, 1923, 2138
HpyF3I CTNAG 8 cut(s) 72, 284, 655, 1130, 1482, 1791, 2192, 2267
HpySE526I ACGT 6 cut(s) 1101, 1525, 1565, 1668, 1696, 1756
Hsp92II CATG 6 cut(s) 269, 424, 448, 752, 1267, 2075
Ksp22I TGATCA 2 cut(s) 1549, 2346
KspAI GTTAAC 1 cut(s) 1218
Kzo9I GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
LmnI GCTCC 1 cut(s) 1326
Lsp1109I GCAGC 3 cut(s) 453, 959, 1400
LweI GCATC 7 cut(s) 234, 538, 545, 731, 845, 2119, 2336
MaeI CTAG 5 cut(s) 318, 800, 1037, 1650, 2392
MaeII ACGT 6 cut(s) 1101, 1525, 1565, 1668, 1696, 1756
MaeIII GTNAC 8 cut(s) 233, 268, 911, 1255, 1377, 1566, 1807, 2276
MalI GATC 8 cut(s) 282, 468, 493, 543, 615, 1551, 1937, 2348
MbiI CCGCTC 1 cut(s) 213
MboI GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
MboII GAAGA 8 cut(s) 49, 137, 327, 553, 980, 1015, 1918, 2369
MfeI CAATTG 1 cut(s) 296
MflI RGATCY 4 cut(s) 280, 466, 491, 613
MhlI GDGCHC 6 cut(s) 139, 189, 381, 736, 1603, 2174
MlyI GAGTC 5 cut(s) 552, 895, 1064, 1591, 1987
MmeI TCCRAC 4 cut(s) 141, 1594, 2142, 2210
Mph1103I ATGCAT 1 cut(s) 898
MslI CAYNNNNRTG 2 cut(s) 275, 2376
MspCI CTTAAG 1 cut(s) 569
MspI CCGG 3 cut(s) 375, 780, 1014
MspR9I CCNGG 3 cut(s) 376, 611, 1928
MunI CAATTG 1 cut(s) 296
MvaI CCWGG 2 cut(s) 611, 1928
MwoI GCNNNNNNNGC 4 cut(s) 210, 261, 1923, 2138
NciI CCSGG 1 cut(s) 376
NdeII GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 6 cut(s) 269, 424, 448, 752, 1267, 2075
NlaIV GGNNCC 4 cut(s) 7, 256, 379, 493
NmuCI GTSAC 3 cut(s) 268, 1377, 2276
NsiI ATGCAT 1 cut(s) 898
NspI RCATGY 3 cut(s) 752, 1267, 2075
NspV TTCGAA 1 cut(s) 459
PaeR7I CTCGAG 1 cut(s) 2320
PceI AGGCCT 2 cut(s) 2025, 2197
PciI ACATGT 1 cut(s) 1263
PfeI GAWTC 8 cut(s) 292, 305, 352, 774, 1227, 1235, 1309, 2294
PkrI GCNGC 3 cut(s) 443, 949, 1415
PleI GAGTC 5 cut(s) 552, 894, 1064, 1590, 1986
PmaCI CACGTG 2 cut(s) 1102, 1526
PmlI CACGTG 2 cut(s) 1102, 1526
PpsI GAGTC 5 cut(s) 552, 894, 1064, 1590, 1986
Ppu21I YACGTR 3 cut(s) 1102, 1526, 1757
PscI ACATGT 1 cut(s) 1263
PshBI ATTAAT 1 cut(s) 177
PsiI TTATAA 1 cut(s) 44
Psp124BI GAGCTC 1 cut(s) 139
Psp1406I AACGTT 1 cut(s) 1668
Psp6I CCWGG 2 cut(s) 609, 1926
PspCI CACGTG 2 cut(s) 1102, 1526
PspGI CCWGG 2 cut(s) 609, 1926
PspN4I GGNNCC 4 cut(s) 7, 256, 379, 493
PspOMI GGGCCC 1 cut(s) 377
PspPI GGNCC 5 cut(s) 6, 377, 378, 638, 926
PsrI GAACNNNNNNTAC 2 cut(s) 2205, 2237
PstI CTGCAG 1 cut(s) 242
PstNI CAGNNNCTG 1 cut(s) 2002
PsuI RGATCY 4 cut(s) 280, 466, 491, 613
RsaI GTAC 5 cut(s) 344, 861, 1843, 2257, 2386
RsaNI GTAC 5 cut(s) 343, 860, 1842, 2256, 2385
RseI CAYNNNNRTG 2 cut(s) 275, 2376
SacI GAGCTC 1 cut(s) 139
SatI GCNGC 3 cut(s) 442, 948, 1414
Sau3AI GATC 8 cut(s) 280, 466, 491, 541, 613, 1549, 1935, 2346
Sau96I GGNCC 5 cut(s) 6, 377, 378, 638, 926
SchI GAGTC 5 cut(s) 552, 895, 1064, 1591, 1987
ScrFI CCNGG 3 cut(s) 376, 611, 1928
SduI GDGCHC 6 cut(s) 139, 189, 381, 736, 1603, 2174
SfaNI GCATC 7 cut(s) 234, 538, 545, 731, 845, 2119, 2336
SfcI CTRYAG 1 cut(s) 238
Sfr274I CTCGAG 1 cut(s) 2320
SfuI TTCGAA 1 cut(s) 459
SinI GGWCC 1 cut(s) 6
SlaI CTCGAG 1 cut(s) 2320
SmiMI CAYNNNNRTG 2 cut(s) 275, 2376
SmlI CTYRAG 5 cut(s) 569, 1201, 1820, 2286, 2320
SmoI CTYRAG 5 cut(s) 569, 1201, 1820, 2286, 2320
SnaBI TACGTA 1 cut(s) 1757
SseBI AGGCCT 2 cut(s) 2025, 2197
SsiI CCGC 4 cut(s) 213, 418, 439, 1512
SspI AATATT 2 cut(s) 175, 1739
SspMI CTAG 5 cut(s) 318, 800, 1037, 1650, 2392
SstI GAGCTC 1 cut(s) 139
StuI AGGCCT 2 cut(s) 2025, 2197
StyD4I CCNGG 3 cut(s) 374, 609, 1926
TaaI ACNGT 6 cut(s) 603, 690, 845, 859, 1377, 1772
TaiI ACGT 6 cut(s) 1104, 1528, 1568, 1671, 1699, 1759
TaqI TCGA 5 cut(s) 181, 459, 966, 1442, 2321
TaqII GACCGA 1 cut(s) 23
TatI WGTACW 3 cut(s) 342, 1841, 2255
TfiI GAWTC 8 cut(s) 292, 305, 352, 774, 1227, 1235, 1309, 2294
TscAI CASTG 2 cut(s) 741, 1153
TseFI GTSAC 3 cut(s) 268, 1377, 2276
TseI GCWGC 3 cut(s) 441, 947, 1413
Tsp45I GTSAC 3 cut(s) 268, 1377, 2276
TspGWI ACGGA 1 cut(s) 999
TspRI CASTG 2 cut(s) 741, 1153
Vha464I CTTAAG 1 cut(s) 569
VneI GTGCAC 2 cut(s) 732, 2170
VpaK11BI GGWCC 1 cut(s) 6
VspI ATTAAT 1 cut(s) 177
XbaI TCTAGA 1 cut(s) 1036
XceI RCATGY 3 cut(s) 752, 1267, 2075
XcmI CCANNNNNNNNNTGG 1 cut(s) 1593
XhoI CTCGAG 1 cut(s) 2320
XspI CTAG 5 cut(s) 318, 800, 1037, 1650, 2392
Zsp2I ATGCAT 1 cut(s) 898
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.