Rw4G002980

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000236
Physical Location & Seq
Reverse (-)
5929034 .. 5937498
8465 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002980.1

Sequence Viewer

Length: 2778 bp
ATGGGGACCGAAGCTTCAGCGAGACTTGTCAGCTTTACTATTACAAGTATATGCCTCATCTTTACTCTTTCTCGGCTTGGTTCTGAATTCAAATATCAGAGTGTAGCTCAAAAGTTGCTCGATGAAGAAGTGACAGCTCTTAATCAAATAATAGAGAAGTTGGAGTTCAACGATATTAATAATCGAGTAACCTCTCAATCATTTTGCAAAAAGAGCGGTCGTGATGGCATCAGATGTAACTGTAGTTTTCAAAACGGCACCATTTGCCATGTCACAGAAATTGCACTGAAGGCTAGAAGTTTGACCGGAGTTATACCTGAAGAACTAGGCAATCTCACACATCTAAGGCAATTGGATCTCAGTAACAATCAATTGACTGGTTCTATACCCGCTAGCTTGGGAAATTTAGCTTCACTTTACAAGCTGAATCTTCACAAAAATCAATTGACCGGGCCAATTCCAGCGAATTTGGGAAATATGAAATTTCAAGTATCATTGGATCTTTCACACAATCAACTGACTGGGCCTATACCAGAGAGCTTAGAGAATTTGTCTTTCATCAGTTCTATTTCTCTGTATGACAACTTTCTCAACGGCGCTATACCAGCATCTTTGGGTACAATGACTTATCTGCGTGAACTGGATTTGAGTAACAATGGAATCTCTGGTACTCTCCCACAAACACTAGGAAAATTGACAATCCTTGAATACTTCTTGGTCCAATCTAACAGTATTACTGGGACAGTACCAAAAAGCTTTGCCAGCCTTACGAGTCTGTATTTCTTTTCCATAGCTGGTAACTATTTGTCTGGCCCTGTACCTGACTTCATAGCCAACTGGACTTCAATCGAAGTGATTTTTCTCAACGGAAACAATTTCAACGGGGAGATACCTGCCGGAATTTTTAATTTATCACACCTAGAGATGTTGGCAATAAGTGACGTCGACTCTCATTTCCAGTTCCCACCATCTACACATGTGACGAAAAGTTTTCGTATATTGATATTAAGAAATTGCTCAATCACGGGTCAAATTCCTTCTTACATTGGAGATATGTCATCATTAAAGAACATAGACTTGAGCTTCAACAGGTTAACGGGTAGAATCCCAGATTCTTTGAAAAACTTAAATTTGAGTTACATGTCTTTTTCAAACAATATGCTTTCTGGCAAAATTCCTGATTGGATTCAAAAAGCAGTATGGAGTAAGATGGATTTTTCAAACAATAATTTTTCAAAGCTAACCTTTAAACCGTCACACAATCTACAACTGAATTTGTTTTCCTGCTGCTGCAACTCCTCAACCTGTCTGCCAAATACGGAATACCCAACCAAGGAGAAGTATTGTCCCCGGGGAAAACATGATTATATTCATGAGGATATTGATTTTAGAGATACAGATAAACGCGCATTTGATGTATATATTCAGGGTGAGAGAAGACTAACGAATTTCAGCATTATAGACAAGGCAGGACGTCCGAATAAAGAATATAAACAAAAGTTCACTGTTGATGTTAATGATAGTACATTAGAGATCCACTTCTACTCTCGATCGCCTGGAGAAGGGACACTTGATGGACCTCTCATATCTGCTATATCCGTAACTCTAGAGCACAAGCAACAACTATCTCCTTTGCAAACAGCGCTCATTATTGTGGCTTCAATCGTAGTTTTCTTGTCGCTTTTATTACTTTTTGCTTGGATGATGGGATGGCTGGGGAATACAGATCACTTACAAGAAATAGATATAGGTATAGAAAAGCCTGTCACCCTCAAACAATTAAAAGTTGCTACTCGGAATTTTAGCAAGAGGAATGAGATTGCTCAAGGGGGTTTTGGGACCGTTTACAAGGCTGAAGTGCAAGGGAAAATTGTAGCCGTGAAGAAACTTTGCTCTCATTGCGAGGAAAGGATCAATGAGTTCATAAATGAATTTTATACCTTAAAAGCAATGAGTCAAGAGAACCTTGTTCAGTTGCTGGACATTTACAACGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGGAGAACAACTCCCTAGCACATGCCTTATTTGACTCAAAGTCCAAATTGAAACTTGATTGGGAAGTTAGGTTTAACATTTGCTTGGGAATAGCTAGGGGATTGGTGTATCTACATGAGCATCCCAGGATGAAGATAGTTCACAGCGACATTAAATCCACTAATATTCTTCTCGATGGAAACCTTAAGGCTAAAATATCAGACTTTGGATTGGCTAGACTTTACACCGAAGATGATCAATTCAAGTTCATCAAAGTAGAAGTGCCACAAGGATATATGGCACCTGAGTATGTTCGAGGAATTGTGACATCTAAAGCTGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGACAAATGCAGGACACAGGGGAGATAGCCAGGAAAGTGAATTTCTTCTAGACACGGCTTATGATTTACATCGAAAAGGAAGGCTGGTGGACTTGGTTGACAAAACCTTGTCTACCAAGTATGATGCAAAACAAGCCATAATCATCTTGAATTTAGCAGTAAAGTGCACCAGTATATCTCCGAGTCTGAGGCCTACTATGTCTGAAGTAGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTCCCCCTGCGCTTAATGATAGTCCCGTTGCTCAAGTTGATTCCTCTGTTTCTATGGAAGCAACATCGAGAGCATCCACATCATCCAATTTGATCAAAGGGGAAGATGAAACAGAACACATTTCTGAGAGTACCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

925

Amino Acids

103.03

Weight (kDa)

6.29

Isoelectric Point (pI)

34.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 90 - 149 7.9e-08 Leucine rich repeat
LRR_4 PF12799 113 - 150 3.1e-06 Leucine Rich repeats (2 copies)
LRR_14 PF23598 204 - 286 3.7e-06 Leucine-rich repeat region
LRR_8 PF13855 210 - 269 6.5e-07 Leucine rich repeat
Malectin PF11721 461 - 532 1.8e-09 Malectin domain
Pkinase PF00069 602 - 867 1.7e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 604 - 870 2e-42 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 945, 1477
Acc36I ACCTGC 1 cut(s) 901
AccB1I GGYRCC 2 cut(s) 257, 2305
AccBSI CCGCTC 1 cut(s) 216
AccI GTMKAC 3 cut(s) 945, 2349, 2501
AccII CGCG 1 cut(s) 1407
AciI CCGC 2 cut(s) 216, 390
AclWI GGATC 4 cut(s) 363, 507, 1528, 1919
AcuI CTGAAG 4 cut(s) 308, 339, 1875, 2613
AcyI GRCGYC 2 cut(s) 942, 1474
AfaI GTAC 6 cut(s) 619, 670, 747, 819, 1525, 2770
AfeI AGCGCT 1 cut(s) 1644
AfiI CCNNNNNNNGG 2 cut(s) 1333, 1562
AflII CTTAAG 1 cut(s) 2210
AflIII ACRYGT 2 cut(s) 976, 1140
AhdI GACNNNNNGTC 1 cut(s) 2065
AjnI CCWGG 3 cut(s) 1555, 2150, 2418
Alw21I GWGCWC 2 cut(s) 1614, 2558
Alw26I GTCTC 1 cut(s) 16
Alw44I GTGCAC 1 cut(s) 2554
AlwI GGATC 4 cut(s) 363, 507, 1528, 1919
AlwNI CAGNNNCTG 1 cut(s) 1978
Ama87I CYCGRG 1 cut(s) 1350
Aor51HI AGCGCT 1 cut(s) 1644
AoxI GGCC 5 cut(s) 452, 524, 811, 1999, 2579
ApaLI GTGCAC 1 cut(s) 2554
ApeKI GCWGC 2 cut(s) 1287, 1290
AseI ATTAAT 1 cut(s) 177
Asp700I GAANNNNTTC 1 cut(s) 2433
AspLEI GCGC 4 cut(s) 599, 1409, 1645, 2650
AspS9I GGNCC 7 cut(s) 6, 452, 524, 718, 812, 1577, 1839
AsuC2I CCSGG 3 cut(s) 451, 1351, 1352
AsuHPI GGTGA 2 cut(s) 1442, 1759
AsuNHI GCTAGC 1 cut(s) 392
AvaI CYCGRG 1 cut(s) 1350
AvaII GGWCC 4 cut(s) 6, 718, 1577, 1839
BaeGI GKGCMC 1 cut(s) 2558
BanI GGYRCC 2 cut(s) 257, 2305
BbsI GAAGAC 1 cut(s) 1444
Bbv12I GWGCWC 2 cut(s) 1614, 2558
BbvI GCAGC 2 cut(s) 1274, 1277
BccI CCATC 7 cut(s) 218, 976, 1204, 1568, 1699, 1704, 2195
BceAI ACGGC 4 cut(s) 271, 610, 1862, 2460
BciT130I CCWGG 3 cut(s) 1557, 2152, 2420
BclI TGATCA 2 cut(s) 2260, 2730
BcnI CCSGG 3 cut(s) 451, 1351, 1352
BcoDI GTCTC 1 cut(s) 16
BfmI CTRYAG 2 cut(s) 241, 2350
BfoI RGCGCY 2 cut(s) 600, 1646
BfrI CTTAAG 1 cut(s) 2210
BfuAI ACCTGC 1 cut(s) 901
BisI GCNGC 2 cut(s) 1288, 1291
BlsI GCNGC 2 cut(s) 1289, 1292
Bme1390I CCNGG 6 cut(s) 451, 1351, 1352, 1557, 2152, 2420
Bme18I GGWCC 4 cut(s) 6, 718, 1577, 1839
BmeRI GACNNNNNGTC 1 cut(s) 2065
BmeT110I CYCGRG 1 cut(s) 1350
BmgT120I GGNCC 7 cut(s) 6, 452, 524, 718, 812, 1577, 1839
BmiI GGNNCC 4 cut(s) 7, 259, 1840, 2307
BmrFI CCNGG 6 cut(s) 451, 1351, 1352, 1557, 2152, 2420
BmrI ACTGGG 2 cut(s) 531, 747
BmsI GCATC 5 cut(s) 237, 617, 2155, 2503, 2720
BmtI GCTAGC 1 cut(s) 396
BmuI ACTGGG 2 cut(s) 531, 747
BpiI GAAGAC 1 cut(s) 1444
BplI GAGNNNNNCTC 4 cut(s) 91, 123, 2021, 2053
BpmI CTGGAG 1 cut(s) 1578
BpuEI CTTGAG 3 cut(s) 1099, 1809, 2655
BpuMI CCSGG 3 cut(s) 451, 1351, 1352
BsaBI GATNNNNATC 1 cut(s) 2457
BsaHI GRCGYC 2 cut(s) 942, 1474
BsaJI CCNNGG 5 cut(s) 1332, 1349, 1350, 1351, 2150
BsaWI WCCGGW 1 cut(s) 305
Bsc4I CCNNNNNNNGG 2 cut(s) 1333, 1562
Bse1I ACTGG 7 cut(s) 382, 526, 645, 742, 842, 958, 2559
Bse3DI GCAATG 2 cut(s) 1897, 1956
Bse8I GATNNNNATC 1 cut(s) 2457
BseBI CCWGG 3 cut(s) 1557, 2152, 2420
BseDI CCNNGG 5 cut(s) 1332, 1349, 1350, 1351, 2150
BseGI GGATG 6 cut(s) 1707, 1715, 2146, 2160, 2711, 2720
BseJI GATNNNNATC 1 cut(s) 2457
BseLI CCNNNNNNNGG 2 cut(s) 1333, 1562
BseMI GCAATG 2 cut(s) 1897, 1956
BseMII CTCAG 4 cut(s) 373, 2301, 2567, 2754
BseNI ACTGG 7 cut(s) 382, 526, 645, 742, 842, 958, 2559
BseRI GAGGAG 2 cut(s) 1288, 2645
BseSI GKGCMC 1 cut(s) 2558
BseXI GCAGC 2 cut(s) 1274, 1277
BseYI CCCAGC 1 cut(s) 1714
Bsh1236I CGCG 1 cut(s) 1407
Bsh1285I CGRYCG 2 cut(s) 220, 1553
BshFI GGCC 5 cut(s) 454, 526, 813, 2001, 2581
BshNI GGYRCC 2 cut(s) 257, 2305
BsiEI CGRYCG 2 cut(s) 220, 1553
BsiHKAI GWGCWC 2 cut(s) 1614, 2558
BsiHKCI CYCGRG 1 cut(s) 1350
BsiSI CCGG 4 cut(s) 306, 450, 897, 1351
BslFI GGGAC 7 cut(s) 19, 754, 1332, 1579, 1852, 2625, 2646
BslI CCNNNNNNNGG 2 cut(s) 1333, 1562
BsmAI GTCTC 1 cut(s) 16
BsmFI GGGAC 7 cut(s) 19, 754, 1332, 1579, 1852, 2625, 2646
BsnI GGCC 5 cut(s) 454, 526, 813, 2001, 2581
BsoBI CYCGRG 1 cut(s) 1350
Bsp1286I GDGCHC 2 cut(s) 1614, 2558
Bsp143I GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
BspACI CCGC 2 cut(s) 216, 390
BspANI GGCC 5 cut(s) 454, 526, 813, 2001, 2581
BspCNI CTCAG 4 cut(s) 372, 2302, 2568, 2755
BspFNI CGCG 1 cut(s) 1407
BspHI TCATGA 1 cut(s) 1372
BspLI GGNNCC 4 cut(s) 7, 259, 1840, 2307
BspMI ACCTGC 1 cut(s) 901
BspOI GCTAGC 1 cut(s) 396
BspPI GGATC 4 cut(s) 363, 507, 1528, 1919
BspT107I GGYRCC 2 cut(s) 257, 2305
BspTI CTTAAG 1 cut(s) 2210
BsrBI CCGCTC 1 cut(s) 216
BsrDI GCAATG 2 cut(s) 1897, 1956
BsrI ACTGG 7 cut(s) 382, 526, 645, 742, 842, 958, 2559
BssECI CCNNGG 5 cut(s) 1332, 1349, 1350, 1351, 2150
BssMI GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
BssNI GRCGYC 2 cut(s) 942, 1474
BssT1I CCWWGG 1 cut(s) 1332
Bst2UI CCWGG 3 cut(s) 1557, 2152, 2420
Bst4CI ACNGT 8 cut(s) 242, 731, 745, 1254, 1507, 1843, 2354, 2380
BstACI GRCGYC 2 cut(s) 942, 1474
BstAFI CTTAAG 1 cut(s) 2210
BstAPI GCANNNNNTGC 1 cut(s) 264
BstC8I GCNNGC 3 cut(s) 394, 763, 2003
BstDEI CTNAG 6 cut(s) 344, 359, 541, 2310, 2576, 2763
BstENI CCTNNNNNAGG 1 cut(s) 1560
BstF5I GGATG 6 cut(s) 1707, 1715, 2146, 2160, 2711, 2720
BstFNI CGCG 1 cut(s) 1407
BstH2I RGCGCY 2 cut(s) 600, 1646
BstHHI GCGC 4 cut(s) 599, 1409, 1645, 2650
BstKTI GATC 8 cut(s) 358, 502, 1536, 1553, 1729, 1914, 2263, 2733
BstMAI GTCTC 1 cut(s) 16
BstMBI GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
BstMCI CGRYCG 2 cut(s) 220, 1553
BstMWI GCNNNNNNNGC 8 cut(s) 213, 264, 290, 605, 762, 1642, 1899, 2522
BstNI CCWGG 3 cut(s) 1557, 2152, 2420
BstNSI RCATGY 3 cut(s) 980, 1144, 2051
BstSCI CCNGG 6 cut(s) 449, 1349, 1350, 1555, 2150, 2418
BstSFI CTRYAG 2 cut(s) 241, 2350
BstSLI GKGCMC 1 cut(s) 2558
BstUI CGCG 1 cut(s) 1407
BstV1I GCAGC 2 cut(s) 1274, 1277
BstV2I GAAGAC 1 cut(s) 1444
BstX2I RGATCY 3 cut(s) 355, 499, 1533
BstYI RGATCY 3 cut(s) 355, 499, 1533
BsuRI GGCC 5 cut(s) 454, 526, 813, 2001, 2581
BtsCI GGATG 6 cut(s) 1707, 1715, 2146, 2160, 2711, 2720
BtsIMutI CAGTG 2 cut(s) 284, 1503
BveI ACCTGC 1 cut(s) 901
Cac8I GCNNGC 3 cut(s) 394, 763, 2003
CaiI CAGNNNCTG 1 cut(s) 1978
CciI TCATGA 1 cut(s) 1372
CfoI GCGC 4 cut(s) 599, 1409, 1645, 2650
Cfr13I GGNCC 7 cut(s) 6, 452, 524, 718, 812, 1577, 1839
Cfr9I CCCGGG 1 cut(s) 1350
Csp6I GTAC 6 cut(s) 618, 669, 746, 818, 1524, 2769
CviAII CATG 7 cut(s) 269, 977, 1141, 1361, 1373, 2048, 2141
CviQI GTAC 6 cut(s) 618, 669, 746, 818, 1524, 2769
DdeI CTNAG 6 cut(s) 344, 359, 541, 2310, 2576, 2763
DpnI GATC 8 cut(s) 357, 501, 1535, 1552, 1728, 1913, 2262, 2732
DpnII GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
DraI TTTAAA 1 cut(s) 1249
DriI GACNNNNNGTC 1 cut(s) 2065
Eam1105I GACNNNNNGTC 1 cut(s) 2065
Eco130I CCWWGG 1 cut(s) 1332
Eco147I AGGCCT 2 cut(s) 2001, 2581
Eco47I GGWCC 4 cut(s) 6, 718, 1577, 1839
Eco47III AGCGCT 1 cut(s) 1644
Eco57I CTGAAG 4 cut(s) 308, 339, 1875, 2613
Eco88I CYCGRG 1 cut(s) 1350
EcoNI CCTNNNNNAGG 1 cut(s) 1560
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 3 cut(s) 1555, 2150, 2418
EcoT14I CCWWGG 1 cut(s) 1332
ErhI CCWWGG 1 cut(s) 1332
FaeI CATG 7 cut(s) 272, 980, 1144, 1364, 1376, 2051, 2144
FalI AAGNNNNNCTT 6 cut(s) 1229, 1261, 1554, 1586, 1950, 1982
FaqI GGGAC 7 cut(s) 19, 754, 1332, 1579, 1852, 2625, 2646
FatI CATG 7 cut(s) 268, 976, 1140, 1360, 1372, 2047, 2140
FauI CCCGC 1 cut(s) 397
FbaI TGATCA 2 cut(s) 2260, 2730
FblI GTMKAC 3 cut(s) 945, 2349, 2501
Fnu4HI GCNGC 2 cut(s) 1288, 1291
FokI GGATG 6 cut(s) 1714, 1722, 2133, 2167, 2698, 2707
Fsp4HI GCNGC 2 cut(s) 1288, 1291
GlaI GCGC 4 cut(s) 598, 1408, 1644, 2649
GluI GCNGC 2 cut(s) 1288, 1291
GsaI CCCAGC 1 cut(s) 1718
GsuI CTGGAG 1 cut(s) 1578
HaeII RGCGCY 2 cut(s) 600, 1646
HaeIII GGCC 5 cut(s) 454, 526, 813, 2001, 2581
HapII CCGG 4 cut(s) 306, 450, 897, 1351
HhaI GCGC 4 cut(s) 599, 1409, 1645, 2650
Hin1I GRCGYC 2 cut(s) 942, 1474
Hin1II CATG 7 cut(s) 272, 980, 1144, 1364, 1376, 2051, 2144
Hin6I GCGC 4 cut(s) 597, 1407, 1643, 2648
HinP1I GCGC 4 cut(s) 597, 1407, 1643, 2648
HincII GTYRAC 3 cut(s) 946, 1095, 2488
HindII GTYRAC 3 cut(s) 946, 1095, 2488
HindIII AAGCTT 2 cut(s) 12, 754
HpaI GTTAAC 1 cut(s) 1095
HpaII CCGG 4 cut(s) 306, 450, 897, 1351
HphI GGTGA 2 cut(s) 1442, 1759
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 5 cut(s) 283, 1047, 1556, 2382, 2463
HpyCH4III ACNGT 8 cut(s) 242, 731, 745, 1254, 1507, 1843, 2354, 2380
HpyCH4IV ACGT 2 cut(s) 942, 1474
HpyCH4V TGCA 9 cut(s) 207, 284, 1293, 1636, 1861, 2005, 2399, 2516, 2556
HpyF10VI GCNNNNNNNGC 8 cut(s) 213, 264, 290, 605, 762, 1642, 1899, 2522
HpyF3I CTNAG 6 cut(s) 344, 359, 541, 2310, 2576, 2763
HpySE526I ACGT 2 cut(s) 942, 1474
Hsp92I GRCGYC 2 cut(s) 942, 1474
Hsp92II CATG 7 cut(s) 272, 980, 1144, 1364, 1376, 2051, 2144
HspAI GCGC 4 cut(s) 597, 1407, 1643, 2648
Ksp22I TGATCA 2 cut(s) 2260, 2730
KspAI GTTAAC 1 cut(s) 1095
Kzo9I GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
Lsp1109I GCAGC 2 cut(s) 1274, 1277
LweI GCATC 5 cut(s) 237, 617, 2155, 2503, 2720
MaeII ACGT 2 cut(s) 942, 1474
MalI GATC 8 cut(s) 357, 501, 1535, 1552, 1728, 1913, 2262, 2732
MbiI CCGCTC 1 cut(s) 216
MboI GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
MfeI CAATTG 3 cut(s) 350, 371, 443
MflI RGATCY 3 cut(s) 355, 499, 1533
MhlI GDGCHC 2 cut(s) 1614, 2558
MlyI GAGTC 5 cut(s) 781, 941, 1963, 2054, 2581
MmeI TCCRAC 1 cut(s) 141
MroXI GAANNNNTTC 1 cut(s) 2433
MslI CAYNNNNRTG 1 cut(s) 1652
MspCI CTTAAG 1 cut(s) 2210
MspI CCGG 4 cut(s) 306, 450, 897, 1351
MspR9I CCNGG 6 cut(s) 451, 1351, 1352, 1557, 2152, 2420
MunI CAATTG 3 cut(s) 350, 371, 443
MvaI CCWGG 3 cut(s) 1557, 2152, 2420
MvnI CGCG 1 cut(s) 1407
MwoI GCNNNNNNNGC 8 cut(s) 213, 264, 290, 605, 762, 1642, 1899, 2522
NciI CCSGG 3 cut(s) 451, 1351, 1352
NdeII GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
NheI GCTAGC 1 cut(s) 392
NlaIII CATG 7 cut(s) 272, 980, 1144, 1364, 1376, 2051, 2144
NlaIV GGNNCC 4 cut(s) 7, 259, 1840, 2307
NmeAIII GCCGAG 1 cut(s) 52
NmuCI GTSAC 7 cut(s) 130, 271, 938, 979, 1254, 1765, 2329
NspI RCATGY 3 cut(s) 980, 1144, 2051
PagI TCATGA 1 cut(s) 1372
PceI AGGCCT 2 cut(s) 2001, 2581
PciI ACATGT 2 cut(s) 976, 1140
PdmI GAANNNNTTC 1 cut(s) 2433
PfeI GAWTC 6 cut(s) 427, 660, 1104, 1112, 1186, 2678
PkrI GCNGC 2 cut(s) 1289, 1292
Ple19I CGATCG 1 cut(s) 1553
PleI GAGTC 5 cut(s) 780, 941, 1962, 2054, 2580
PpsI GAGTC 5 cut(s) 780, 941, 1962, 2054, 2580
PscI ACATGT 2 cut(s) 976, 1140
PshBI ATTAAT 1 cut(s) 177
Psp6I CCWGG 3 cut(s) 1555, 2150, 2418
PspFI CCCAGC 1 cut(s) 1714
PspGI CCWGG 3 cut(s) 1555, 2150, 2418
PspN4I GGNNCC 4 cut(s) 7, 259, 1840, 2307
PspPI GGNCC 7 cut(s) 6, 452, 524, 718, 812, 1577, 1839
PsrI GAACNNNNNNTAC 2 cut(s) 2589, 2621
PstNI CAGNNNCTG 1 cut(s) 1978
PsuI RGATCY 3 cut(s) 355, 499, 1533
PvuI CGATCG 1 cut(s) 1553
RsaI GTAC 6 cut(s) 619, 670, 747, 819, 1525, 2770
RsaNI GTAC 6 cut(s) 618, 669, 746, 818, 1524, 2769
RseI CAYNNNNRTG 1 cut(s) 1652
SalI GTCGAC 1 cut(s) 944
SatI GCNGC 2 cut(s) 1288, 1291
Sau3AI GATC 8 cut(s) 355, 499, 1533, 1550, 1726, 1911, 2260, 2730
Sau96I GGNCC 7 cut(s) 6, 452, 524, 718, 812, 1577, 1839
SchI GAGTC 5 cut(s) 781, 941, 1963, 2054, 2581
ScrFI CCNGG 6 cut(s) 451, 1351, 1352, 1557, 2152, 2420
SduI GDGCHC 2 cut(s) 1614, 2558
SfaNI GCATC 5 cut(s) 237, 617, 2155, 2503, 2720
SfcI CTRYAG 2 cut(s) 241, 2350
SinI GGWCC 4 cut(s) 6, 718, 1577, 1839
SmaI CCCGGG 1 cut(s) 1352
SmiMI CAYNNNNRTG 1 cut(s) 1652
SmlI CTYRAG 4 cut(s) 1078, 1824, 2210, 2670
SmoI CTYRAG 4 cut(s) 1078, 1824, 2210, 2670
SseBI AGGCCT 2 cut(s) 2001, 2581
SsiI CCGC 2 cut(s) 216, 390
SspI AATATT 1 cut(s) 2191
StuI AGGCCT 2 cut(s) 2001, 2581
StyD4I CCNGG 6 cut(s) 449, 1349, 1350, 1555, 2150, 2418
StyI CCWWGG 1 cut(s) 1332
TaaI ACNGT 8 cut(s) 242, 731, 745, 1254, 1507, 1843, 2354, 2380
TaiI ACGT 2 cut(s) 945, 1477
TaqI TCGA 9 cut(s) 120, 184, 849, 945, 1549, 2199, 2320, 2461, 2705
TaqII GACCGA 1 cut(s) 23
TatI WGTACW 1 cut(s) 1523
TfiI GAWTC 6 cut(s) 427, 660, 1104, 1112, 1186, 2678
TscAI CASTG 2 cut(s) 291, 1510
TseFI GTSAC 7 cut(s) 130, 271, 938, 979, 1254, 1765, 2329
TseI GCWGC 2 cut(s) 1287, 1290
Tsp45I GTSAC 7 cut(s) 130, 271, 938, 979, 1254, 1765, 2329
TspGWI ACGGA 3 cut(s) 882, 1334, 1588
TspMI CCCGGG 1 cut(s) 1350
TspRI CASTG 2 cut(s) 291, 1510
Vha464I CTTAAG 1 cut(s) 2210
VneI GTGCAC 1 cut(s) 2554
VpaK11BI GGWCC 4 cut(s) 6, 718, 1577, 1839
VspI ATTAAT 1 cut(s) 177
XagI CCTNNNNNAGG 1 cut(s) 1560
XbaI TCTAGA 2 cut(s) 1606, 2437
XceI RCATGY 3 cut(s) 980, 1144, 2051
XmaI CCCGGG 1 cut(s) 1350
XmiI GTMKAC 3 cut(s) 945, 2349, 2501
XmnI GAANNNNTTC 1 cut(s) 2433
ZraI GACGTC 2 cut(s) 943, 1475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.