Rroxscaffold_1G00075170

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000236
Physical Location & Seq
Forward (+)
95947400 .. 95955423
8024 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00075170.1

Sequence Viewer

Length: 2292 bp
ATGCTTTCAGTTTCAAGGGCAACGGCAGCTTTGCTTTGGAATCTTGATCATCGCATCTCAAGCTATTCATCTTCCGAGATAGAGGATGCAGAGGAATGCTTGAAACAGTACCGCGCTTTGCCGCGGGATTTGTTGTTGCTAACAAATGAAATTGGAAGCTTAAGAGATTGTGCTCCAAGGTGGAGAGATCGGAAAGATGGGCAGCTTGGAAGTAATCTGGGTGACAACTTTCTCAAGGGTTCTATACCAGCATCTTTGGGTAAACTGACTAATCTCGTGTTGCTGCGTTTGCATGACAATGGAATATCTGGTACTCTCCCACAAGCACTTGGAAATTTGAAAAACCTTATGTTCTTTTCTGTAGCTGGTAACTATTTGTCTGGCCCATTACCTGACTTCATAGCCAACTGGGCTGAAATCTTTGAACTGGTAATTAGTGATGTAGGAGACTCTCATTTCCAGTTCCTACCGTCTAATATGACGAGCAGCTTACTTACTTTGATACTAAGAAACTGCTCAATCTCCGGTCAAATTCCTTCTGACATGGGAAATATGTCATGGTTATCGAACCTAGACCTGAGCTTCAACAGCTTAACGGGTGGAATCCCAGATTCTTTGAAAAACATAAGATTGAGTTTAATGTCCTTTTCAAACAATATGCTTTCTGGGAAAATTCCTGATTGGGTTCAAAATGCAACCTGGAGAGCAATATTTGTTAGGACGGACCCAACAACGGGGAAGTATTGTCCACAGGGAAAACCTAAGTACCATTCATTGTTTATAAATTGTGGCGGTGGGGAAACACCTTTTGATGGAAATGTTTATGAACAAGATAATGAAACGTCACAATATTACTTGAGTCCAAAAGGAAACTGGGCTCGGAGCAGTGCTGGAAGCATTGTGGTTCTAGAGACTGATCTCAATTTTAGTAGATTCTTAAAAACCGTGAGATGTGGGCTTTCTTCTGAAGCAGCTTTATACAATAATGCTCGCACTTCTCCTGTCTCTCTAAAATATTACGGGTTCTGTTTACGTAAAGGCAAATACAATGTAACACTTCACTTTGCTGAAATTGTTGATGATGAAAAGGATTACAGAAGTACAGAGAAACGCGTATTTGATGTATATATTCAGGGTGAGAGGAAACTAAAGGATTTCAACATTATAGACAAGGCAGGACGTCCCAATAATGTATCTCAAGTTAATTTCACGGATGTCAGTGTAACTGATAGTAGTCATAGTACATTAGAGATCCACTTCTACTCGGCTGGAAAGGGGCTTGATCAGGGACCTCTAATATCTGCTATATCCGTAACTCCAGAAATAGATATAGGTCTAGAAAAGCCTGTCACCCTCAAACAATTAAAAGATGCTACTCGGAATTTTAGCAAGAGGAACGAGATTGGTCAAGGGGGTTTTGGGACCGTTTACAAGGCTGAAGTGCAAGGGAAAATTGTAGCTGTGAAGAAACTTTCCTCTCATTCAGAGGAAAGGATCAATCAGTTGAAAAATGAGTTTTATACTTTAAAATCAATGAGTCAAGAGAACCTTGTTCAGTTGCTGGACGTTTACAACGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGCAAAACAAGTCCCTTGCATGCGCCTTATTTGACTCAAAGTCAAAACTGAAACTTAATTGGGAAGCTAGGGACATTAAATCAGCTAATATTCTTCTCGATGGAAACCTCAAGGCTAAAATATCAGACTTTGGATTGGCAAGCCTTTACACCGAAAATGATCAATTCAAGTTCATCAAAGTAGAAGTGCCACAGGGATATATGGCACCTGAGTATGTTCGAGGAATTGTGACATCTAAAGCTGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGACAAATGCAGGACACAGGCGAGATAGCCAGGAAAGTGAATTTCTTTTAGACACGGCTTATGGTTTACAGCAAAAAGGAAGGCTGGTGGACTTGGTTGACCTAACCTTGCCTACCAAGTATGATGCAAAACAAGCCATCATCATCTTGAATTTAGCAGTAAAGTGCACCAGTATATCCCCAACTCTGAGGCCTACTATGTCTGAAGTTGTGAGTGTTCTCGTTGGCGACAAAAAAATTGAGGAGATTTGTCCCCCTGCTCTGAATGATAGTCACATTGTTCAGGTTGATTCCTCTGTTTCTATGGAAGTAACCTCCGGAGCATCCACATCATCTAATGTGATCAAAGCCCCTGTGCCGTCGTGGGTTGGCTTGAGATCGAAGAAAGTAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

763

Amino Acids

84.53

Weight (kDa)

7.53

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 258 - 436 2e-27 Malectin domain
Pkinase PF00069 463 - 546 4.7e-13 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 465 - 557 2.3e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 560 - 703 1.3e-18 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 560 - 705 2e-15 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 782
AatII GACGTC 1 cut(s) 1183
AccB1I GGYRCC 1 cut(s) 1813
AccI GTMKAC 1 cut(s) 1857
AccII CGCG 3 cut(s) 114, 124, 1113
AccIII TCCGGA 1 cut(s) 2213
AciI CCGC 4 cut(s) 112, 122, 124, 792
AclWI GGATC 2 cut(s) 1246, 1502
AcsI RAATTY 6 cut(s) 334, 531, 672, 1381, 1937, 2047
AcuI CTGAAG 3 cut(s) 987, 1458, 2121
AcyI GRCGYC 1 cut(s) 1180
AfaI GTAC 5 cut(s) 110, 313, 767, 1102, 1243
AfiI CCNNNNNNNGG 3 cut(s) 733, 734, 812
AflII CTTAAG 1 cut(s) 160
AflIII ACRYGT 1 cut(s) 1111
AhdI GACNNNNNGTC 1 cut(s) 1648
AjnI CCWGG 2 cut(s) 698, 1926
Alw21I GWGCWC 2 cut(s) 175, 2066
Alw26I GTCTC 3 cut(s) 441, 905, 1009
Alw44I GTGCAC 1 cut(s) 2062
AlwI GGATC 2 cut(s) 1246, 1502
AlwNI CAGNNNCTG 1 cut(s) 1561
Aor13HI TCCGGA 1 cut(s) 2213
AoxI GGCC 3 cut(s) 382, 1582, 2087
ApaLI GTGCAC 1 cut(s) 2062
ApeKI GCWGC 5 cut(s) 26, 202, 283, 486, 971
ApoI RAATTY 6 cut(s) 334, 531, 672, 1381, 1937, 2047
AspLEI GCGC 2 cut(s) 116, 1634
AspS9I GGNCC 4 cut(s) 383, 724, 1289, 1422
AsuHPI GGTGA 3 cut(s) 233, 1148, 1342
AvaII GGWCC 3 cut(s) 724, 1289, 1422
BaeGI GKGCMC 1 cut(s) 2066
BaeI ACNNNNGTAYC 2 cut(s) 749, 782
BanI GGYRCC 1 cut(s) 1813
BanII GRGCYC 1 cut(s) 880
BauI CACGAG 1 cut(s) 275
Bbv12I GWGCWC 2 cut(s) 175, 2066
BbvI GCAGC 5 cut(s) 38, 214, 270, 498, 983
BccI CCATC 4 cut(s) 191, 806, 1703, 2042
BceAI ACGGC 3 cut(s) 39, 1968, 2239
BciT130I CCWGG 2 cut(s) 700, 1928
BclI TGATCA 4 cut(s) 46, 1282, 1768, 2238
BcoDI GTCTC 3 cut(s) 441, 905, 1009
BfaI CTAG 4 cut(s) 572, 908, 1337, 1677
BfmI CTRYAG 2 cut(s) 360, 1858
BfrI CTTAAG 1 cut(s) 160
BglI GCCNNNNNGGC 1 cut(s) 410
BisI GCNGC 6 cut(s) 27, 122, 203, 284, 487, 972
BlsI GCNGC 6 cut(s) 28, 123, 204, 285, 488, 973
Bme1390I CCNGG 2 cut(s) 700, 1928
Bme18I GGWCC 3 cut(s) 724, 1289, 1422
BmeRI GACNNNNNGTC 1 cut(s) 1648
BmgT120I GGNCC 4 cut(s) 383, 724, 1289, 1422
BmiI GGNNCC 4 cut(s) 726, 1290, 1423, 1815
BmrFI CCNGG 2 cut(s) 700, 1928
BmrI ACTGGG 2 cut(s) 418, 883
BmsI GCATC 6 cut(s) 63, 76, 260, 1360, 2011, 2228
BmuI ACTGGG 2 cut(s) 418, 883
BpmI CTGGAG 2 cut(s) 721, 1302
Bpu10I CCTNAGC 1 cut(s) 578
BpuEI CTTGAG 6 cut(s) 43, 218, 877, 1182, 1703, 2290
BsaAI YACGTR 1 cut(s) 1034
BsaHI GRCGYC 1 cut(s) 1180
BsaJI CCNNGG 2 cut(s) 122, 176
BsaWI WCCGGW 2 cut(s) 524, 2213
Bsc4I CCNNNNNNNGG 3 cut(s) 733, 734, 812
Bse1I ACTGG 5 cut(s) 413, 432, 460, 878, 2067
BseAI TCCGGA 1 cut(s) 2213
BseBI CCWGG 2 cut(s) 700, 1928
BseDI CCNNGG 2 cut(s) 122, 176
BseGI GGATG 3 cut(s) 91, 1219, 2219
BseLI CCNNNNNNNGG 3 cut(s) 733, 734, 812
BseMII CTCAG 3 cut(s) 569, 1809, 2075
BseNI ACTGG 5 cut(s) 413, 432, 460, 878, 2067
BseRI GAGGAG 1 cut(s) 2153
BseSI GKGCMC 1 cut(s) 2066
BseXI GCAGC 5 cut(s) 38, 214, 270, 498, 983
Bsh1236I CGCG 3 cut(s) 114, 124, 1113
BshFI GGCC 3 cut(s) 384, 1584, 2089
BshNI GGYRCC 1 cut(s) 1813
BsiHKAI GWGCWC 2 cut(s) 175, 2066
BsiSI CCGG 2 cut(s) 525, 2214
BslFI GGGAC 6 cut(s) 1167, 1302, 1435, 1606, 1694, 2133
BslI CCNNNNNNNGG 3 cut(s) 733, 734, 812
BsmAI GTCTC 3 cut(s) 441, 905, 1009
BsmFI GGGAC 6 cut(s) 1167, 1302, 1435, 1606, 1694, 2133
BsmI GAATGC 1 cut(s) 101
BsnI GGCC 3 cut(s) 384, 1584, 2089
Bsp1286I GDGCHC 3 cut(s) 175, 880, 2066
Bsp13I TCCGGA 1 cut(s) 2213
Bsp143I GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
BspACI CCGC 4 cut(s) 112, 122, 124, 792
BspANI GGCC 3 cut(s) 384, 1584, 2089
BspCNI CTCAG 3 cut(s) 570, 1810, 2076
BspEI TCCGGA 1 cut(s) 2213
BspFNI CGCG 3 cut(s) 114, 124, 1113
BspLI GGNNCC 4 cut(s) 726, 1290, 1423, 1815
BspPI GGATC 2 cut(s) 1246, 1502
BspT107I GGYRCC 1 cut(s) 1813
BspTI CTTAAG 1 cut(s) 160
BsrI ACTGG 5 cut(s) 413, 432, 460, 878, 2067
BssECI CCNNGG 2 cut(s) 122, 176
BssMI GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
BssNI GRCGYC 1 cut(s) 1180
BssSI CACGAG 1 cut(s) 275
BssT1I CCWWGG 1 cut(s) 176
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 2 cut(s) 700, 1928
Bst4CI ACNGT 6 cut(s) 108, 471, 946, 1426, 1862, 1888
BstACI GRCGYC 1 cut(s) 1180
BstAFI CTTAAG 1 cut(s) 160
BstBAI YACGTR 1 cut(s) 1034
BstC8I GCNNGC 4 cut(s) 991, 1586, 1630, 1750
BstDEI CTNAG 5 cut(s) 506, 578, 762, 1818, 2084
BstDSI CCRYGG 1 cut(s) 122
BstF5I GGATG 3 cut(s) 91, 1219, 2219
BstFNI CGCG 3 cut(s) 114, 124, 1113
BstHHI GCGC 2 cut(s) 116, 1634
BstKTI GATC 9 cut(s) 49, 190, 919, 1254, 1285, 1497, 1771, 2241, 2276
BstMAI GTCTC 3 cut(s) 441, 905, 1009
BstMBI GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
BstMWI GCNNNNNNNGC 6 cut(s) 26, 60, 289, 410, 588, 2030
BstNI CCWGG 2 cut(s) 700, 1928
BstNSI RCATGY 1 cut(s) 1632
BstSCI CCNGG 2 cut(s) 698, 1926
BstSFI CTRYAG 2 cut(s) 360, 1858
BstSLI GKGCMC 1 cut(s) 2066
BstSNI TACGTA 1 cut(s) 1034
BstUI CGCG 3 cut(s) 114, 124, 1113
BstV1I GCAGC 5 cut(s) 38, 214, 270, 498, 983
BstX2I RGATCY 1 cut(s) 1251
BstYI RGATCY 1 cut(s) 1251
BsuRI GGCC 3 cut(s) 384, 1584, 2089
BtgI CCRYGG 1 cut(s) 122
BtgZI GCGATG 1 cut(s) 35
BtsCI GGATG 3 cut(s) 91, 1219, 2219
BtsI GCAGTG 1 cut(s) 892
BtsIMutI CAGTG 2 cut(s) 892, 1225
Cac8I GCNNGC 4 cut(s) 991, 1586, 1630, 1750
CaiI CAGNNNCTG 1 cut(s) 1561
CfoI GCGC 2 cut(s) 116, 1634
Cfr13I GGNCC 4 cut(s) 383, 724, 1289, 1422
Cfr42I CCGCGG 1 cut(s) 125
Csp6I GTAC 5 cut(s) 109, 312, 766, 1101, 1242
CviAII CATG 4 cut(s) 293, 544, 558, 1629
CviQI GTAC 5 cut(s) 109, 312, 766, 1101, 1242
DdeI CTNAG 5 cut(s) 506, 578, 762, 1818, 2084
DpnI GATC 9 cut(s) 48, 189, 918, 1253, 1284, 1496, 1770, 2240, 2275
DpnII GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
DraI TTTAAA 1 cut(s) 1527
DriI GACNNNNNGTC 1 cut(s) 1648
Eam1105I GACNNNNNGTC 1 cut(s) 1648
Eco105I TACGTA 1 cut(s) 1034
Eco130I CCWWGG 1 cut(s) 176
Eco147I AGGCCT 2 cut(s) 1584, 2089
Eco24I GRGCYC 1 cut(s) 880
Eco47I GGWCC 3 cut(s) 724, 1289, 1422
Eco57I CTGAAG 3 cut(s) 987, 1458, 2121
EcoO109I RGGNCCY 1 cut(s) 1289
EcoRII CCWGG 2 cut(s) 698, 1926
EcoT14I CCWWGG 1 cut(s) 176
EcoT38I GRGCYC 1 cut(s) 880
ErhI CCWWGG 1 cut(s) 176
FaeI CATG 4 cut(s) 296, 547, 561, 1632
FalI AAGNNNNNCTT 2 cut(s) 1533, 1565
FaqI GGGAC 6 cut(s) 1167, 1302, 1435, 1606, 1694, 2133
FatI CATG 4 cut(s) 292, 543, 557, 1628
FauI CCCGC 1 cut(s) 117
FbaI TGATCA 4 cut(s) 46, 1282, 1768, 2238
FblI GTMKAC 1 cut(s) 1857
Fnu4HI GCNGC 6 cut(s) 27, 122, 203, 284, 487, 972
FokI GGATG 3 cut(s) 98, 1226, 2206
FriOI GRGCYC 1 cut(s) 880
Fsp4HI GCNGC 6 cut(s) 27, 122, 203, 284, 487, 972
FspBI CTAG 4 cut(s) 572, 908, 1337, 1677
GlaI GCGC 2 cut(s) 115, 1633
GluI GCNGC 6 cut(s) 27, 122, 203, 284, 487, 972
GsuI CTGGAG 2 cut(s) 721, 1302
HaeIII GGCC 3 cut(s) 384, 1584, 2089
HapII CCGG 2 cut(s) 525, 2214
HhaI GCGC 2 cut(s) 116, 1634
Hin1I GRCGYC 1 cut(s) 1180
Hin1II CATG 4 cut(s) 296, 547, 561, 1632
Hin6I GCGC 2 cut(s) 114, 1632
HinP1I GCGC 2 cut(s) 114, 1632
HincII GTYRAC 1 cut(s) 1996
HindII GTYRAC 1 cut(s) 1996
HindIII AAGCTT 1 cut(s) 157
HinfI GANTC 9 cut(s) 40, 449, 603, 611, 859, 933, 1537, 1643, 2186
HpaII CCGG 2 cut(s) 525, 2214
HphI GGTGA 3 cut(s) 233, 1148, 1342
Hpy188III TCNNGA 9 cut(s) 44, 677, 908, 1319, 1337, 1541, 1706, 2044, 2214
Hpy99I CGWCG 1 cut(s) 2260
HpyAV CCTTC 3 cut(s) 546, 1890, 1971
HpyCH4III ACNGT 6 cut(s) 108, 471, 946, 1426, 1862, 1888
HpyCH4IV ACGT 4 cut(s) 842, 1033, 1180, 1566
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 60, 289, 410, 588, 2030
HpyF3I CTNAG 5 cut(s) 506, 578, 762, 1818, 2084
HpySE526I ACGT 4 cut(s) 842, 1033, 1180, 1566
Hsp92I GRCGYC 1 cut(s) 1180
Hsp92II CATG 4 cut(s) 296, 547, 561, 1632
HspAI GCGC 2 cut(s) 114, 1632
Kpn2I TCCGGA 1 cut(s) 2213
Ksp22I TGATCA 4 cut(s) 46, 1282, 1768, 2238
KspI CCGCGG 1 cut(s) 125
Kzo9I GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
LmnI GCTCC 3 cut(s) 178, 882, 2216
Lsp1109I GCAGC 5 cut(s) 38, 214, 270, 498, 983
LweI GCATC 6 cut(s) 63, 76, 260, 1360, 2011, 2228
MaeI CTAG 4 cut(s) 572, 908, 1337, 1677
MaeII ACGT 4 cut(s) 842, 1033, 1180, 1566
MalI GATC 9 cut(s) 48, 189, 918, 1253, 1284, 1496, 1770, 2240, 2275
MboI GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
MboII GAAGA 5 cut(s) 63, 954, 1477, 1694, 2290
MflI RGATCY 1 cut(s) 1251
MhlI GDGCHC 3 cut(s) 175, 880, 2066
MluI ACGCGT 1 cut(s) 1111
MlyI GAGTC 4 cut(s) 443, 868, 1546, 1637
MroI TCCGGA 1 cut(s) 2213
MseI TTAA 9 cut(s) 161, 593, 638, 938, 1203, 1364, 1526, 1665, 1686
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 124
MspCI CTTAAG 1 cut(s) 160
MspI CCGG 2 cut(s) 525, 2214
MspR9I CCNGG 2 cut(s) 700, 1928
Mva1269I GAATGC 1 cut(s) 101
MvaI CCWGG 2 cut(s) 700, 1928
MvnI CGCG 3 cut(s) 114, 124, 1113
MwoI GCNNNNNNNGC 6 cut(s) 26, 60, 289, 410, 588, 2030
NdeII GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
NlaIII CATG 4 cut(s) 296, 547, 561, 1632
NlaIV GGNNCC 4 cut(s) 726, 1290, 1423, 1815
NmeAIII GCCGAG 1 cut(s) 1244
NmuCI GTSAC 5 cut(s) 221, 843, 1348, 1837, 2168
NspI RCATGY 1 cut(s) 1632
PaeI GCATGC 1 cut(s) 1632
PceI AGGCCT 2 cut(s) 1584, 2089
PctI GAATGC 1 cut(s) 101
PfeI GAWTC 5 cut(s) 40, 603, 611, 933, 2186
PkrI GCNGC 6 cut(s) 28, 123, 204, 285, 488, 973
PleI GAGTC 4 cut(s) 443, 867, 1545, 1637
PpsI GAGTC 4 cut(s) 443, 867, 1545, 1637
Ppu21I YACGTR 1 cut(s) 1034
PpuMI RGGWCCY 1 cut(s) 1289
PsiI TTATAA 1 cut(s) 782
Psp5II RGGWCCY 1 cut(s) 1289
Psp6I CCWGG 2 cut(s) 698, 1926
PspGI CCWGG 2 cut(s) 698, 1926
PspN4I GGNNCC 4 cut(s) 726, 1290, 1423, 1815
PspPI GGNCC 4 cut(s) 383, 724, 1289, 1422
PspPPI RGGWCCY 1 cut(s) 1289
PstNI CAGNNNCTG 1 cut(s) 1561
PsuI RGATCY 1 cut(s) 1251
RsaI GTAC 5 cut(s) 110, 313, 767, 1102, 1243
RsaNI GTAC 5 cut(s) 109, 312, 766, 1101, 1242
RseI CAYNNNNRTG 1 cut(s) 297
SacII CCGCGG 1 cut(s) 125
SaqAI TTAA 9 cut(s) 161, 593, 638, 938, 1203, 1364, 1526, 1665, 1686
SatI GCNGC 6 cut(s) 27, 122, 203, 284, 487, 972
Sau3AI GATC 9 cut(s) 46, 187, 916, 1251, 1282, 1494, 1768, 2238, 2273
Sau96I GGNCC 4 cut(s) 383, 724, 1289, 1422
SchI GAGTC 4 cut(s) 443, 868, 1546, 1637
ScrFI CCNGG 2 cut(s) 700, 1928
SduI GDGCHC 3 cut(s) 175, 880, 2066
SfaNI GCATC 6 cut(s) 63, 76, 260, 1360, 2011, 2228
SfcI CTRYAG 2 cut(s) 360, 1858
Sfr303I CCGCGG 1 cut(s) 125
SgrBI CCGCGG 1 cut(s) 125
SinI GGWCC 3 cut(s) 724, 1289, 1422
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 7 cut(s) 58, 160, 233, 856, 1197, 1718, 2269
SmoI CTYRAG 7 cut(s) 58, 160, 233, 856, 1197, 1718, 2269
SnaBI TACGTA 1 cut(s) 1034
SphI GCATGC 1 cut(s) 1632
SseBI AGGCCT 2 cut(s) 1584, 2089
SsiI CCGC 4 cut(s) 112, 122, 124, 792
SspI AATATT 4 cut(s) 711, 851, 1016, 1699
SspMI CTAG 4 cut(s) 572, 908, 1337, 1677
StuI AGGCCT 2 cut(s) 1584, 2089
StyD4I CCNGG 2 cut(s) 698, 1926
StyI CCWWGG 1 cut(s) 176
TaaI ACNGT 6 cut(s) 108, 471, 946, 1426, 1862, 1888
TaiI ACGT 4 cut(s) 845, 1036, 1183, 1569
TaqI TCGA 4 cut(s) 566, 1707, 1828, 2276
TatI WGTACW 2 cut(s) 1100, 1241
TauI GCSGC 1 cut(s) 124
TfiI GAWTC 5 cut(s) 40, 603, 611, 933, 2186
Tru1I TTAA 9 cut(s) 161, 593, 638, 938, 1203, 1364, 1526, 1665, 1686
Tru9I TTAA 9 cut(s) 161, 593, 638, 938, 1203, 1364, 1526, 1665, 1686
TscAI CASTG 2 cut(s) 892, 1225
TseFI GTSAC 5 cut(s) 221, 843, 1348, 1837, 2168
TseI GCWGC 5 cut(s) 26, 202, 283, 486, 971
Tsp45I GTSAC 5 cut(s) 221, 843, 1348, 1837, 2168
TspDTI ATGAA 9 cut(s) 57, 162, 388, 762, 840, 852, 1098, 1617, 1771
TspGWI ACGGA 3 cut(s) 737, 1226, 1300
TspRI CASTG 2 cut(s) 892, 1225
Vha464I CTTAAG 1 cut(s) 160
VneI GTGCAC 1 cut(s) 2062
VpaK11BI GGWCC 3 cut(s) 724, 1289, 1422
XapI RAATTY 6 cut(s) 334, 531, 672, 1381, 1937, 2047
XbaI TCTAGA 2 cut(s) 907, 1336
XceI RCATGY 1 cut(s) 1632
XcmI CCANNNNNNNNNTGG 1 cut(s) 870
XmiI GTMKAC 1 cut(s) 1857
XspI CTAG 4 cut(s) 572, 908, 1337, 1677
ZraI GACGTC 1 cut(s) 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.