Rroxscaffold_5G00337150

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000236
Physical Location & Seq
Reverse (-)
5190872 .. 5200854
9983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00337150.1

Sequence Viewer

Length: 2688 bp
ATGGGGACCGAAGCTTCAGCGAGACTTGTCAGCTTTGCTATTATAAGTATATGCCTCATCTTTACTCTTTCTCAGCTTGGTTCTGAATTCAAATATCAGAGTATAGCTCAAAAGTTGCCCGATGAAGAAGTGACAGCTCTTAATCAAATAATAGAGAAGTTGGAGTTCAACGATATTAATCGAGTGCCCTCTAAATCATTTTGCAAAAAGAGCGCTCGTGATGGCATCAGATGTAACTGCAGTTTTCAAAACGGCACCATTTGCCATGTCACAGAAATGGATCTTAGTAGGAATCAATTGACTGGTTCTATACCTGCTAGCTTGGGGAATTTATCTTCGTTGTACAAGCTGATTCTTTACAGCAATCAATTGACCGGGCCCATTCCAGAGGGTTTGGGAAATATGAAATTTCAAGTCCGCATGGATCATACTTTGCACCGCAACATGGCTGTTAGCTTCGAACTGGATCTTTCAAACAATCAATTAAGTGGACCCATACCAACCACTTGGAATGTGGATGTCTCTGATGTGGCATCTGATCCAGATGCCTACCGACTCTTCAAGCTTAAGCAATACGACAGGGCTGATGTTAACCTAGATTTCTACCTGGATCTTTCAAACAATCAATTGACTGGGCCTATACCAGAGAGCTTAGGGAACTTGTCTTTCATCAGTTCTATTTATCTGCGGAACAACTTTCTCGACGGCTATATACCAGCATCTTTGGGTGCATTGACTTATCTGCGTGGACTGTATTTGGATAGGAATAGAATCTCTGGTACTCTCCCCCAAACACTAGGAAATTTGACGCTCCTTGAAATCTTTTTCATAGCTGGTAACTATTTTTCTGGCCCTTTACCTGACTTCATAGCCAACTGGACTTCAATCGCGTATCTTTTTCTCAACGGAAACAATTTCAATGGGAAGATACCTGCCGGAATTTTTAATTTACGACACCTAGAAATGTTGTCTTTTTCGAACAATGCGCTTTCTGGGAAAATTCCCGATTGGATTCAATATGCAGCATGGAGTAAGATGACAGACACAATCAAGGAGAAGTATTGTCCCAAGGGAAAACCTAATTACCATTCATTGTTCATAAATTGTGGTGGTGGGGATACATCTGTTGGTGGAAATGTTTATGATCAAGATAATGATACGTCACTATTTTACCTGAGTCCAAAAGGAAACTGGGCTCGGAGTAGCGTTGGAAACATCTATGACTATTCCAATGGCAATTCTAGTAAATTGTTAAAACGCGATGGATGTTTGCTTCCATCTGAAGCGTCTTTATACGATAAAGCTCGCATTTCCCTTGTCTCTCTAAAATATTACGGGTTCTGTTTACGTAAAGGCAATTACAATGTAACACTTCACTTTGCTGAAATTGTTGATGAGGATGCAGATGTTCATAAGGATGTTGATTTTAGAAGTACGGATAAACGCGTATTTGATGTATATATTCAGGGTGAGAGAAAACTAAAGGATTTCAACATTATAGACAAGGCAAGAGGTCCGAATAAAGAACTTACAAAAAATTTCACGGCTGATGTGAATGATGGTACATTAGAGATCCACTTCTACTCTCGATCGGCTGGAGAAGGGAGACTTGATGGACCTCTCATATCTGCTATATCCGTAACTCCAGAAATAGATATAGGTATAGAAAAGCCTGTCACCCTCAAACAATTAAAAGATGCTACTCGGAATTTTAGCAAGAGGAATGAGATTGCTCAAGGGGGTTTTGGGACCGTTTACAAGGCTGAAGTGCAAGGGAAAATTGTAGCCGTGAAGAAACTTCGCTCTCATTGCCAGGAAAGGATCAATGAGTTCATAAATGAATTTTATACCTTAAAAGCAATGAGTCAAGAGAACCTTGTTCAGTTGCTGGACATTTACAACGCAAAAGGCCTGCATTTGCTCATCTATGAATATATGGAGAACAACTCCCTTGCACATGCTTTATTTGACTCAAAGTCCAAATTGAAACTTGATTGGGAAGTTAGGTTTAACATTTGCTTAGGAATAGCTAGGGGATTGGTGTATCTACATGAGCATCCCAGGATGAAGATAGTTCACAGCGACATTAAATCCACTAATATTCTTCTCGATGGAAACCTTAAGGCTAAAATATCAGACTTTGGATTGGCAAGACTTTACACCGAAGATGATCAATTCAAGTTCATCAAAGTAGAAGTGCCACAAGGATATATGGCACCTGAGTATGTTCGAGGAATTGTGACATCTAAAGCCGATGTCTACAGTTTTGGGGTGGTTATACTTGAAACTGTTAGTGGAAGGACAAATGCAGGATACAGGCGAGATAGCCAGGAAAGTGAATTTCTTCTAGACACGGCTTATGATTTACATCGGAAAGGAAGGTTGGTGGACTTGGTTGACAAAACCTTGTCCAACAAGTATGATGCAAAACAAGCCATAATCATTTTGAATTTAGCAGTAAAGTGCACCAATATATCTCCAACTCTGAGGCCTACTATGTCTGAAGTAGTGAGTGTTCTCGTTGGCGACCAAAAAATTGAGGAGATTTGTACTCCTGCTCTTAGTGATAGTCACCTTGCTCAAGTTGACTCCTCTGTTTCTATGGAAGCAACCTCGAGAGCATCCACATCATCCAATTTGATCAAAGGGGAAGATGAAACAGAACACATTTCTGAGAGTACCCCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_1 Malectin Pkinase Pkinase_Tyr

Protein Analysis

895

Amino Acids

100.11

Weight (kDa)

6.16

Isoelectric Point (pI)

29.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Malectin PF11721 364 - 545 5.4e-27 Malectin domain
Pkinase PF00069 572 - 837 6.4e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 574 - 840 5e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000236)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800 FvH4_3g00800
malus_domestica MD00G1124000.v1.1 MD10G1333200.v1.1 MD10G1333300.v1.1 MD10G1333800.v1.1 MD10G1333900.v1.1 MD10G1334000.v1.1 MD10G1334300.v1.1 MD10G1334500.v1.1 MD10G1334600.v1.1
prunus_persica Prupe.6G125000_v2.0.a1 Prupe.6G126100_v2.0.a1
pyrus_communis pycom10g28270 pycom10g28410 pycom10g28420 pycom10g28430
rosa_chinensis RchiOBHm_Chr1g0337521 RchiOBHm_Chr1g0337731 RchiOBHm_Chr3g0485891 RchiOBHm_Chr4g0391431 RchiOBHm_Chr4g0391551 RchiOBHm_Chr4g0391571 RchiOBHm_Chr4g0391641 RchiOBHm_Chr4g0391651 RchiOBHm_Chr4g0391661 RchiOBHm_Chr4g0391791 RchiOBHm_Chr4g0409301 RchiOBHm_Chr5g0001061 RchiOBHm_Chr5g0001201 RchiOBHm_Chr5g0001251 RchiOBHm_Chr5g0001261 RchiOBHm_Chr5g0001271 RchiOBHm_Chr5g0040441 RchiOBHm_Chr6g0279971
rosa_laevigata RLG00000009856 RLG00000009862 RLG00000029911 RLG00000030125 RLG00000030918
rosa_multiflora Rmu_co8113804.1_g000001 Rmu_sc0000255.1_g000042 Rmu_sc0001174.1_g000026 Rmu_sc0001706.1_g000014 Rmu_sc0001706.1_g000017 Rmu_sc0001706.1_g000032 Rmu_sc0001706.1_g000039 Rmu_sc0002377.1_g000003 Rmu_sc0002772.1_g000022 Rmu_sc0004189.1_g000005 Rmu_sc0004189.1_g000025 Rmu_sc0004189.1_g000033 Rmu_sc0004862.1_g000015 Rmu_sc0004876.1_g000018 Rmu_sc0014338.1_g000004 Rmu_sc0024491.1_g000002 Rmu_sc0042682.1_g000001
rosa_roxburghii Rroxscaffold_1G00005720 Rroxscaffold_1G00040220 Rroxscaffold_1G00040230 Rroxscaffold_1G00040260 Rroxscaffold_1G00050150 Rroxscaffold_1G00050170 Rroxscaffold_1G00075050 Rroxscaffold_1G00075070 Rroxscaffold_1G00075080 Rroxscaffold_1G00075110 Rroxscaffold_1G00075170 Rroxscaffold_1G00075230 Rroxscaffold_3G00274520 Rroxscaffold_4G00314190 Rroxscaffold_4G00314200 Rroxscaffold_5G00334510 Rroxscaffold_5G00337150 Rroxscaffold_5G00353810 Rroxscaffold_6G00396280 Rroxscaffold_7G00198980
rosa_rugosa Rorug03G0336100 Rorug04G0092200 Rorug04G0389300 Rorug04G0389500 Rorug04G0389500 Rorug05G0185000
rosa_samantha Rh1CG145400 Rh3AG271500 Rh4AG037800 Rh4AG037900 Rh4AG038600 Rh4AG155300 Rh4BG031900 Rh4BG032700 Rh4BG152800 Rh4BG153100 Rh4CG040900 Rh4CG041000 Rh4CG041200 Rh4DG035300 Rh4DG035400 Rh4DG148200 Rh5AG008600 Rh5AG008700 Rh5AG009000 Rh5AG009300 Rh5AG272100 Rh5BG010700 Rh5BG010800 Rh5BG011200 Rh5BG011700 Rh5BG011800 Rh5BG012100 Rh5BG012200 Rh5BG012300 Rh5CG009300 Rh5CG009800 Rh5CG009900 Rh5CG308100 Rh5CG308200 Rh5CG309500 Rh5DG009500 Rh5DG009900 Rh5DG284400 Rh5DG284500
rosa_wichuraiana Rw1G012750 Rw4G002940 Rw4G002980 Rw4G012680 Rw5G000870 Rw5G000940 Rw5G000970 Rw5G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 44
Acc36I ACCTGC 2 cut(s) 322, 940
AccB1I GGYRCC 2 cut(s) 254, 2215
AccI GTMKAC 1 cut(s) 2259
AccII CGCG 3 cut(s) 890, 1260, 1446
AciI CCGC 3 cut(s) 418, 439, 688
AclWI GGATC 7 cut(s) 288, 432, 474, 533, 618, 1567, 1829
AcuI CTGAAG 3 cut(s) 1302, 1785, 2523
AfaI GTAC 6 cut(s) 344, 781, 1435, 1564, 2551, 2680
AfeI AGCGCT 1 cut(s) 214
AfiI CCNNNNNNNGG 1 cut(s) 445
AflII CTTAAG 2 cut(s) 566, 2120
AflIII ACRYGT 1 cut(s) 1444
AhdI GACNNNNNGTC 1 cut(s) 1975
AjnI CCWGG 4 cut(s) 606, 1812, 2060, 2328
AjuI GAANNNNNNNTTGG 2 cut(s) 2366, 2398
Alw21I GWGCWC 1 cut(s) 2468
Alw26I GTCTC 4 cut(s) 16, 526, 1324, 1600
Alw44I GTGCAC 1 cut(s) 2464
AlwI GGATC 7 cut(s) 288, 432, 474, 533, 618, 1567, 1829
AlwNI CAGNNNCTG 1 cut(s) 1888
Ama87I CYCGRG 1 cut(s) 2614
Aor51HI AGCGCT 1 cut(s) 214
AoxI GGCC 5 cut(s) 377, 635, 850, 1909, 2489
ApaI GGGCCC 1 cut(s) 381
ApaLI GTGCAC 1 cut(s) 2464
ApeKI GCWGC 1 cut(s) 1022
AseI ATTAAT 1 cut(s) 177
Asp700I GAANNNNTTC 1 cut(s) 2343
AspLEI GCGC 2 cut(s) 215, 988
AspS9I GGNCC 9 cut(s) 6, 377, 378, 491, 635, 851, 1514, 1616, 1749
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 3 cut(s) 1481, 1669, 2564
AsuII TTCGAA 2 cut(s) 459, 977
AsuNHI GCTAGC 1 cut(s) 317
AvaI CYCGRG 1 cut(s) 2614
AvaII GGWCC 5 cut(s) 6, 491, 1514, 1616, 1749
BaeGI GKGCMC 3 cut(s) 189, 381, 2468
BaeI ACNNNNGTAYC 2 cut(s) 1110, 1143
BanI GGYRCC 2 cut(s) 254, 2215
BanII GRGCYC 2 cut(s) 381, 1198
BauI CACGAG 1 cut(s) 216
Bbv12I GWGCWC 1 cut(s) 2468
BbvI GCAGC 1 cut(s) 1034
BccI CCATC 6 cut(s) 215, 1256, 1285, 1553, 1607, 2105
BceAI ACGGC 5 cut(s) 268, 721, 1560, 1772, 2370
BciT130I CCWGG 4 cut(s) 608, 1814, 2062, 2330
BciVI GTATCC 2 cut(s) 1111, 2306
BclI TGATCA 3 cut(s) 1144, 2170, 2640
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 4 cut(s) 16, 526, 1324, 1600
BfaI CTAG 8 cut(s) 318, 596, 797, 959, 1242, 2031, 2348, 2686
BfmI CTRYAG 2 cut(s) 238, 2260
BfoI RGCGCY 1 cut(s) 216
BfrI CTTAAG 2 cut(s) 566, 2120
BfuAI ACCTGC 2 cut(s) 322, 940
BfuI GTATCC 2 cut(s) 1111, 2306
BisI GCNGC 1 cut(s) 1023
BlsI GCNGC 1 cut(s) 1024
Bme1390I CCNGG 5 cut(s) 376, 608, 1814, 2062, 2330
Bme18I GGWCC 5 cut(s) 6, 491, 1514, 1616, 1749
BmeRI GACNNNNNGTC 1 cut(s) 1975
BmeT110I CYCGRG 1 cut(s) 2614
BmgT120I GGNCC 9 cut(s) 6, 377, 378, 491, 635, 851, 1514, 1616, 1749
BmiI GGNNCC 6 cut(s) 7, 256, 379, 493, 1750, 2217
BmrFI CCNGG 5 cut(s) 376, 608, 1814, 2062, 2330
BmrI ACTGGG 2 cut(s) 642, 1201
BmsI GCATC 9 cut(s) 234, 535, 542, 728, 1390, 1687, 2065, 2413, 2630
BmtI GCTAGC 1 cut(s) 321
BmuI ACTGGG 2 cut(s) 642, 1201
BplI GAGNNNNNCTC 4 cut(s) 91, 123, 1931, 1963
BpmI CTGGAG 2 cut(s) 1617, 1629
Bpu10I CCTNAGC 2 cut(s) 652, 2020
Bpu14I TTCGAA 2 cut(s) 459, 977
BpuEI CTTGAG 2 cut(s) 1719, 2565
BpuMI CCSGG 1 cut(s) 376
BsaAI YACGTR 1 cut(s) 1349
BsaBI GATNNNNATC 2 cut(s) 177, 2367
BsaJI CCNNGG 2 cut(s) 1068, 2060
Bsc4I CCNNNNNNNGG 1 cut(s) 445
Bse1I ACTGG 5 cut(s) 307, 468, 637, 881, 1196
Bse3DI GCAATG 2 cut(s) 1807, 1866
Bse8I GATNNNNATC 2 cut(s) 177, 2367
BseBI CCWGG 4 cut(s) 608, 1814, 2062, 2330
BseDI CCNNGG 2 cut(s) 1068, 2060
BseGI GGATG 8 cut(s) 523, 1271, 1405, 1423, 2056, 2070, 2621, 2630
BseJI GATNNNNATC 2 cut(s) 177, 2367
BseLI CCNNNNNNNGG 1 cut(s) 445
BseMI GCAATG 2 cut(s) 1807, 1866
BseMII CTCAG 5 cut(s) 86, 1166, 2211, 2477, 2664
BseNI ACTGG 5 cut(s) 307, 468, 637, 881, 1196
BseRI GAGGAG 2 cut(s) 2555, 2581
BseSI GKGCMC 3 cut(s) 189, 381, 2468
BseXI GCAGC 1 cut(s) 1034
Bsh1236I CGCG 3 cut(s) 890, 1260, 1446
Bsh1285I CGRYCG 1 cut(s) 1592
BshFI GGCC 5 cut(s) 379, 637, 852, 1911, 2491
BshNI GGYRCC 2 cut(s) 254, 2215
BsiEI CGRYCG 1 cut(s) 1592
BsiHKAI GWGCWC 1 cut(s) 2468
BsiHKCI CYCGRG 1 cut(s) 2614
BsiSI CCGG 2 cut(s) 375, 936
BslFI GGGAC 3 cut(s) 19, 1050, 1762
BslI CCNNNNNNNGG 1 cut(s) 445
BsmAI GTCTC 4 cut(s) 16, 526, 1324, 1600
BsmFI GGGAC 3 cut(s) 19, 1050, 1762
BsnI GGCC 5 cut(s) 379, 637, 852, 1911, 2491
BsoBI CYCGRG 1 cut(s) 2614
Bsp119I TTCGAA 2 cut(s) 459, 977
Bsp120I GGGCCC 1 cut(s) 377
Bsp1286I GDGCHC 4 cut(s) 189, 381, 1198, 2468
Bsp1407I TGTACA 1 cut(s) 342
BspACI CCGC 3 cut(s) 418, 439, 688
BspANI GGCC 5 cut(s) 379, 637, 852, 1911, 2491
BspCNI CTCAG 5 cut(s) 85, 1167, 2212, 2478, 2665
BspFNI CGCG 3 cut(s) 890, 1260, 1446
BspLI GGNNCC 6 cut(s) 7, 256, 379, 493, 1750, 2217
BspMAI CTGCAG 1 cut(s) 242
BspMI ACCTGC 2 cut(s) 322, 940
BspOI GCTAGC 1 cut(s) 321
BspPI GGATC 7 cut(s) 288, 432, 474, 533, 618, 1567, 1829
BspT104I TTCGAA 2 cut(s) 459, 977
BspT107I GGYRCC 2 cut(s) 254, 2215
BspTI CTTAAG 2 cut(s) 566, 2120
BsrDI GCAATG 2 cut(s) 1807, 1866
BsrGI TGTACA 1 cut(s) 342
BsrI ACTGG 5 cut(s) 307, 468, 637, 881, 1196
BssECI CCNNGG 2 cut(s) 1068, 2060
BssSI CACGAG 1 cut(s) 216
BssT1I CCWWGG 1 cut(s) 1068
Bst2BI CACGAG 1 cut(s) 216
Bst2UI CCWGG 4 cut(s) 608, 1814, 2062, 2330
Bst4CI ACNGT 4 cut(s) 753, 1753, 2264, 2290
Bst6I CTCTTC 1 cut(s) 563
BstAFI CTTAAG 2 cut(s) 566, 2120
BstAPI GCANNNNNTGC 1 cut(s) 261
BstAUI TGTACA 1 cut(s) 342
BstBAI YACGTR 1 cut(s) 1349
BstBI TTCGAA 2 cut(s) 459, 977
BstC8I GCNNGC 3 cut(s) 319, 1306, 1913
BstDEI CTNAG 9 cut(s) 72, 284, 652, 1175, 2020, 2220, 2486, 2561, 2673
BstF5I GGATG 8 cut(s) 523, 1271, 1405, 1423, 2056, 2070, 2621, 2630
BstFNI CGCG 3 cut(s) 890, 1260, 1446
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 2 cut(s) 215, 988
BstMAI GTCTC 4 cut(s) 16, 526, 1324, 1600
BstMCI CGRYCG 1 cut(s) 1592
BstMWI GCNNNNNNNGC 4 cut(s) 210, 261, 1809, 2432
BstNI CCWGG 4 cut(s) 608, 1814, 2062, 2330
BstNSI RCATGY 1 cut(s) 1961
BstSCI CCNGG 5 cut(s) 374, 606, 1812, 2060, 2328
BstSFI CTRYAG 2 cut(s) 238, 2260
BstSLI GKGCMC 3 cut(s) 189, 381, 2468
BstSNI TACGTA 1 cut(s) 1349
BstUI CGCG 3 cut(s) 890, 1260, 1446
BstV1I GCAGC 1 cut(s) 1034
BstX2I RGATCY 4 cut(s) 280, 466, 610, 1572
BstXI CCANNNNNNTGG 1 cut(s) 507
BstYI RGATCY 4 cut(s) 280, 466, 610, 1572
BsuI GTATCC 2 cut(s) 1111, 2306
BsuRI GGCC 5 cut(s) 379, 637, 852, 1911, 2491
BtgZI GCGATG 1 cut(s) 1275
BtsCI GGATG 8 cut(s) 523, 1271, 1405, 1423, 2056, 2070, 2621, 2630
BveI ACCTGC 2 cut(s) 322, 940
Cac8I GCNNGC 3 cut(s) 319, 1306, 1913
CaiI CAGNNNCTG 1 cut(s) 1888
CfoI GCGC 2 cut(s) 215, 988
Cfr13I GGNCC 9 cut(s) 6, 377, 378, 491, 635, 851, 1514, 1616, 1749
CseI GACGC 2 cut(s) 817, 1275
Csp6I GTAC 6 cut(s) 343, 780, 1434, 1563, 2550, 2679
CviAII CATG 6 cut(s) 266, 421, 445, 1026, 1958, 2051
CviQI GTAC 6 cut(s) 343, 780, 1434, 1563, 2550, 2679
DdeI CTNAG 9 cut(s) 72, 284, 652, 1175, 2020, 2220, 2486, 2561, 2673
DriI GACNNNNNGTC 1 cut(s) 1975
Eam1104I CTCTTC 1 cut(s) 563
Eam1105I GACNNNNNGTC 1 cut(s) 1975
EarI CTCTTC 1 cut(s) 563
Eco105I TACGTA 1 cut(s) 1349
Eco130I CCWWGG 1 cut(s) 1068
Eco147I AGGCCT 2 cut(s) 1911, 2491
Eco24I GRGCYC 2 cut(s) 381, 1198
Eco47I GGWCC 5 cut(s) 6, 491, 1514, 1616, 1749
Eco47III AGCGCT 1 cut(s) 214
Eco57I CTGAAG 3 cut(s) 1302, 1785, 2523
Eco88I CYCGRG 1 cut(s) 2614
EcoRI GAATTC 1 cut(s) 86
EcoRII CCWGG 4 cut(s) 606, 1812, 2060, 2328
EcoT14I CCWWGG 1 cut(s) 1068
EcoT38I GRGCYC 2 cut(s) 381, 1198
ErhI CCWWGG 1 cut(s) 1068
FaeI CATG 6 cut(s) 269, 424, 448, 1029, 1961, 2054
FalI AAGNNNNNCTT 4 cut(s) 1593, 1625, 1860, 1892
FaqI GGGAC 3 cut(s) 19, 1050, 1762
FatI CATG 6 cut(s) 265, 420, 444, 1025, 1957, 2050
FbaI TGATCA 3 cut(s) 1144, 2170, 2640
FblI GTMKAC 1 cut(s) 2259
Fnu4HI GCNGC 1 cut(s) 1023
FokI GGATG 8 cut(s) 530, 1278, 1412, 1430, 2043, 2077, 2608, 2617
FriOI GRGCYC 2 cut(s) 381, 1198
Fsp4HI GCNGC 1 cut(s) 1023
FspBI CTAG 8 cut(s) 318, 596, 797, 959, 1242, 2031, 2348, 2686
GlaI GCGC 2 cut(s) 214, 987
GluI GCNGC 1 cut(s) 1023
GsuI CTGGAG 2 cut(s) 1617, 1629
HaeII RGCGCY 1 cut(s) 216
HaeIII GGCC 5 cut(s) 379, 637, 852, 1911, 2491
HapII CCGG 2 cut(s) 375, 936
HgaI GACGC 2 cut(s) 817, 1275
HhaI GCGC 2 cut(s) 215, 988
Hin1II CATG 6 cut(s) 269, 424, 448, 1029, 1961, 2054
Hin6I GCGC 2 cut(s) 213, 986
HinP1I GCGC 2 cut(s) 213, 986
HincII GTYRAC 3 cut(s) 592, 2398, 2587
HindII GTYRAC 3 cut(s) 592, 2398, 2587
HindIII AAGCTT 2 cut(s) 12, 563
HinfI GANTC 9 cut(s) 292, 352, 555, 771, 1012, 1177, 1864, 1970, 2588
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 2 cut(s) 375, 936
HphI GGTGA 3 cut(s) 1481, 1669, 2564
Hpy99I CGWCG 1 cut(s) 707
HpyAV CCTTC 3 cut(s) 1595, 2292, 2373
HpyCH4III ACNGT 4 cut(s) 753, 1753, 2264, 2290
HpyCH4IV ACGT 2 cut(s) 1160, 1348
HpyF10VI GCNNNNNNNGC 4 cut(s) 210, 261, 1809, 2432
HpyF3I CTNAG 9 cut(s) 72, 284, 652, 1175, 2020, 2220, 2486, 2561, 2673
HpySE526I ACGT 2 cut(s) 1160, 1348
Hsp92II CATG 6 cut(s) 269, 424, 448, 1029, 1961, 2054
HspAI GCGC 2 cut(s) 213, 986
Ksp22I TGATCA 3 cut(s) 1144, 2170, 2640
KspAI GTTAAC 1 cut(s) 592
LmnI GCTCC 1 cut(s) 816
Lsp1109I GCAGC 1 cut(s) 1034
LweI GCATC 9 cut(s) 234, 535, 542, 728, 1390, 1687, 2065, 2413, 2630
MaeI CTAG 8 cut(s) 318, 596, 797, 959, 1242, 2031, 2348, 2686
MaeII ACGT 2 cut(s) 1160, 1348
MfeI CAATTG 3 cut(s) 296, 368, 626
MflI RGATCY 4 cut(s) 280, 466, 610, 1572
MhlI GDGCHC 4 cut(s) 189, 381, 1198, 2468
MluI ACGCGT 1 cut(s) 1444
MlyI GAGTC 5 cut(s) 549, 1186, 1873, 1964, 2582
MmeI TCCRAC 4 cut(s) 141, 1189, 2436, 2504
MroXI GAANNNNTTC 1 cut(s) 2343
MslI CAYNNNNRTG 2 cut(s) 275, 1416
MspCI CTTAAG 2 cut(s) 566, 2120
MspI CCGG 2 cut(s) 375, 936
MspR9I CCNGG 5 cut(s) 376, 608, 1814, 2062, 2330
MunI CAATTG 3 cut(s) 296, 368, 626
MvaI CCWGG 4 cut(s) 608, 1814, 2062, 2330
MvnI CGCG 3 cut(s) 890, 1260, 1446
MwoI GCNNNNNNNGC 4 cut(s) 210, 261, 1809, 2432
NciI CCSGG 1 cut(s) 376
NheI GCTAGC 1 cut(s) 317
NlaIII CATG 6 cut(s) 269, 424, 448, 1029, 1961, 2054
NlaIV GGNNCC 6 cut(s) 7, 256, 379, 493, 1750, 2217
NmuCI GTSAC 6 cut(s) 130, 268, 1161, 1675, 2239, 2570
NspI RCATGY 1 cut(s) 1961
NspV TTCGAA 2 cut(s) 459, 977
PaeR7I CTCGAG 1 cut(s) 2614
PceI AGGCCT 2 cut(s) 1911, 2491
PdmI GAANNNNTTC 1 cut(s) 2343
PfeI GAWTC 4 cut(s) 292, 352, 771, 1012
PkrI GCNGC 1 cut(s) 1024
Ple19I CGATCG 1 cut(s) 1592
PleI GAGTC 5 cut(s) 549, 1185, 1872, 1964, 2582
PpsI GAGTC 5 cut(s) 549, 1185, 1872, 1964, 2582
Ppu21I YACGTR 1 cut(s) 1349
PshBI ATTAAT 1 cut(s) 177
PsiI TTATAA 1 cut(s) 44
Psp6I CCWGG 4 cut(s) 606, 1812, 2060, 2328
PspGI CCWGG 4 cut(s) 606, 1812, 2060, 2328
PspN4I GGNNCC 6 cut(s) 7, 256, 379, 493, 1750, 2217
PspOMI GGGCCC 1 cut(s) 377
PspPI GGNCC 9 cut(s) 6, 377, 378, 491, 635, 851, 1514, 1616, 1749
PsrI GAACNNNNNNTAC 2 cut(s) 2499, 2531
PstI CTGCAG 1 cut(s) 242
PstNI CAGNNNCTG 1 cut(s) 1888
PsuI RGATCY 4 cut(s) 280, 466, 610, 1572
PvuI CGATCG 1 cut(s) 1592
RsaI GTAC 6 cut(s) 344, 781, 1435, 1564, 2551, 2680
RsaNI GTAC 6 cut(s) 343, 780, 1434, 1563, 2550, 2679
RseI CAYNNNNRTG 2 cut(s) 275, 1416
SatI GCNGC 1 cut(s) 1023
Sau96I GGNCC 9 cut(s) 6, 377, 378, 491, 635, 851, 1514, 1616, 1749
SchI GAGTC 5 cut(s) 549, 1186, 1873, 1964, 2582
ScrFI CCNGG 5 cut(s) 376, 608, 1814, 2062, 2330
SduI GDGCHC 4 cut(s) 189, 381, 1198, 2468
SfaNI GCATC 9 cut(s) 234, 535, 542, 728, 1390, 1687, 2065, 2413, 2630
SfcI CTRYAG 2 cut(s) 238, 2260
Sfr274I CTCGAG 1 cut(s) 2614
SfuI TTCGAA 2 cut(s) 459, 977
SinI GGWCC 5 cut(s) 6, 491, 1514, 1616, 1749
SlaI CTCGAG 1 cut(s) 2614
SmiMI CAYNNNNRTG 2 cut(s) 275, 1416
SmlI CTYRAG 5 cut(s) 566, 1734, 2120, 2580, 2614
SmoI CTYRAG 5 cut(s) 566, 1734, 2120, 2580, 2614
SnaBI TACGTA 1 cut(s) 1349
SseBI AGGCCT 2 cut(s) 1911, 2491
SsiI CCGC 3 cut(s) 418, 439, 688
SspI AATATT 2 cut(s) 1331, 2101
SspMI CTAG 8 cut(s) 318, 596, 797, 959, 1242, 2031, 2348, 2686
StuI AGGCCT 2 cut(s) 1911, 2491
StyD4I CCNGG 5 cut(s) 374, 606, 1812, 2060, 2328
StyI CCWWGG 1 cut(s) 1068
TaaI ACNGT 4 cut(s) 753, 1753, 2264, 2290
TaiI ACGT 2 cut(s) 1163, 1351
TaqI TCGA 8 cut(s) 181, 459, 702, 977, 1588, 2109, 2230, 2615
TaqII GACCGA 1 cut(s) 23
TatI WGTACW 2 cut(s) 342, 2549
TfiI GAWTC 4 cut(s) 292, 352, 771, 1012
TseFI GTSAC 6 cut(s) 130, 268, 1161, 1675, 2239, 2570
TseI GCWGC 1 cut(s) 1022
Tsp45I GTSAC 6 cut(s) 130, 268, 1161, 1675, 2239, 2570
TspGWI ACGGA 3 cut(s) 921, 1451, 1627
Vha464I CTTAAG 2 cut(s) 566, 2120
VneI GTGCAC 1 cut(s) 2464
VpaK11BI GGWCC 5 cut(s) 6, 491, 1514, 1616, 1749
VspI ATTAAT 1 cut(s) 177
XbaI TCTAGA 1 cut(s) 2347
XceI RCATGY 1 cut(s) 1961
XcmI CCANNNNNNNNNTGG 2 cut(s) 511, 1188
XhoI CTCGAG 1 cut(s) 2614
XmiI GTMKAC 1 cut(s) 2259
XmnI GAANNNNTTC 1 cut(s) 2343
XspI CTAG 8 cut(s) 318, 596, 797, 959, 1242, 2031, 2348, 2686
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.