MD13G1057000.v1.1

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000078
Physical Location & Seq
Reverse (-)
3990668 .. 3993203
2536 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1057000.v1.1.491

Sequence Viewer

Length: 1698 bp
ATGTTGTACATTTTCTATCATCCATTCCAGCTCTGTTATTCAGTTAGTGAGAGGGGAGGCAGAAAATCAAAACTATTGAGAGCGGTCGAGAGGTGTGTTCTAACTTATATACCAAAAGGAAAAAAAAAAGGGAGAATGAGTTGGTTGGGATTCGAAATGCCTCTAATTACTACTTCAAGCTTTCTGTGTCTGTTCCTAGTAATTCTTTTAGCTCTCATAAAAAACTTTCAGAAACTATGGTGGACTCCAAATCGCATACAGAATCGGATGGCTGCCCAGGGAGTCCATGGCCCTTCTTACAAATTTATCCATGGAAACACCAAAGAAATATGGAGCATGAAAAGGGAAACAATGGGCAGGCCTAGAAGTTTAACCCATGACATATTCTCTGCAGTACAACCTCATATTCATGCATGGAGCAAGATCTATGGGAAGAATTTTCTTCAGTGGTATGGTGTTCAACCACAGATGATCATTGCGGAACCCGAGTTGTGCAAAGAGGTACTAAACAACAAAGATAGAAACTATCGGAAACAAAGGTCCCAAGGCTATATCAAAAAGCTATTAGGAGACAGCATTTCGATGGCAGAAGGTGAAAAGTGGGTGAAACTAAGAAAGTTGGCGCATCATGCCTTCCATGGAGAGAGCTTAAAAAATATGATTCCTGAAATGATAAAGAGTTCTGAGACAATGGTAAAAAGGTGGAAAACTTATGAAGGCAAGGAGATTGAGGTGAATGAAGAATTTAGGTTTTTCACCTCAGAAGTGATTTCTAGGACAGCATTTGGCAGCAGCTTCCTTGAAGGAAAGGACATTTTTGAAATGTTGAGGGAGTTAACCTCCTTAATATTCAGAAACACTTTCAAACTCAGGCTCCCTGGCATCAGTATGTTCTATAAAACCAGTGATGAGATAAAATCAGACAAGTTCGAGAAGGGAATACGCGACAGCATAACAGAGATTGTTCGGAAAAGAGAAAAGACGGCAATGACTGGAGAAGCAGACGGTTTTGGAAGTGATTATCTTGGAATACTTTTAAAGGCTCATCATGATGCCCACGAGAAGCAGCGGATTTCAGTGGACGATTTAGTCGATGAGTGCAAGACGTTTTACTTTGCTGGACAAGAAACCACTAACTCTTTGCTTGCTTGGACCGTCTTTCTTCTGGCTCTTCATACAGATTGGCAAGAGGAAGCTAGAAAGGAGGTGCTAGAATTGTTTGGTAAAGATGAAACTCCAAACTCAGATGGCCTCAACAAACTAAAAACGATGGGTATGATCATCAATGAGTCTCTAAGGTTATATCCTCCTGTTGTGTCCCTTACTAGGGAATCTCTAAAGGAAGTTAGACTAGGACAGGTCGTTGTTCCCGCTGATGTTGAGTTGCACGTCTCAAATCTGGCACTTCACCATGAACCTAAATATTGGGGAAAAGACGTGCAACTTTTCAAACCGGAGAGATTCTCAGAAGGGGTTGCGAAAGCTACTAACAACAACGCAGTCGCATTCATGCCCTTTGGAATGGGACCTCGAACTTGTGTGGGCATGAACTTCGCAATCATTGAAGCAAAGATTGCTTTGTCGATGATTCTGCAACGCTACAGTTTCACTCTTTCCCCGGGTTATGTGCACTCTCCCATTCAGTTTCTGACAGTTCGCCCGCAACATGGAGTTCAAGTGATCCTACACCCAGTGTGA

Protein Analysis

566

Amino Acids

65.15

Weight (kDa)

9.39

Isoelectric Point (pI)

33.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 136 - 555 5.4e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000078)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g15240 FvH4_3g15270 FvH4_3g15280 FvH4_3g32750 FvH4_3g32760 FvH4_3g32760 FvH4_3g32880 FvH4_4g08190 FvH4_4g29160 FvH4_4g29370 FvH4_4g29400 FvH4_4g29420 FvH4_4g29420 FvH4_4g29420 FvH4_4g29430 FvH4_4g29440 FvH4_4g29450 FvH4_4g29450 FvH4_4g29450 FvH4_4g29460 FvH4_4g29461 FvH4_4g29462 FvH4_4g29463 FvH4_4g29480 FvH4_4g29481 FvH4_4g29482 FvH4_4g29483 FvH4_4g29484 FvH4_6g26720 FvH4_6g26720
malus_domestica MD00G1065200.v1.1 MD06G1145800.v1.1 MD06G1146000.v1.1 MD06G1146700.v1.1 MD13G1031000.v1.1 MD13G1033900.v1.1 MD13G1057000.v1.1 MD13G1057100.v1.1 MD13G1057200.v1.1 MD13G1057400.v1.1 MD13G1057800.v1.1 MD13G1058000.v1.1 MD13G1058100.v1.1 MD13G1058200.v1.1 MD13G1058300.v1.1 MD13G1058500.v1.1 MD13G1058600.v1.1 MD13G1058700.v1.1 MD16G1035700.v1.1 MD16G1056400.v1.1 MD16G1056600.v1.1 MD16G1056700.v1.1 MD16G1056900.v1.1 MD16G1058100.v1.1
prunus_persica Prupe.1G291100_v2.0.a1 Prupe.1G291200_v2.0.a1 Prupe.1G291500_v2.0.a1 Prupe.1G291600_v2.0.a1 Prupe.1G291700_v2.0.a1 Prupe.1G291800_v2.0.a1 Prupe.1G291900_v2.0.a1 Prupe.1G292000_v2.0.a1 Prupe.1G292100_v2.0.a1 Prupe.1G292400_v2.0.a1 Prupe.1G292500_v2.0.a1 Prupe.1G292600_v2.0.a1 Prupe.1G292700_v2.0.a1 Prupe.1G292800_v2.0.a1 Prupe.1G296000_v2.0.a1 Prupe.1G296100_v2.0.a1 Prupe.1G296300_v2.0.a1 Prupe.1G296600_v2.0.a1 Prupe.1G296900_v2.0.a1 Prupe.1G534600_v2.0.a1 Prupe.5G046700_v2.0.a1 Prupe.8G021800_v2.0.a1 Prupe.8G026400_v2.0.a1
pyrus_communis pycom06g13510 pycom06g13530 pycom06g13540 pycom06g13570 pycom06g13590 pycom06g13620 pycom13g02710 pycom13g02930 pycom13g05100 pycom13g05110 pycom13g05140 pycom13g05160 pycom13g05170 pycom13g05190 pycom13g05200 pycom13g05210 pycom13g05220 pycom16g03060 pycom16g04940 pycom16g04960 pycom16g04990 pycom16g05000 pycom16g05080
rosa_chinensis RchiOBHm_Chr1g0337981 RchiOBHm_Chr1g0337991 RchiOBHm_Chr1g0339431 RchiOBHm_Chr4g0402401 RchiOBHm_Chr4g0420571 RchiOBHm_Chr4g0420601 RchiOBHm_Chr4g0420611 RchiOBHm_Chr4g0420621 RchiOBHm_Chr4g0420631 RchiOBHm_Chr4g0437211 RchiOBHm_Chr4g0437221 RchiOBHm_Chr4g0437251 RchiOBHm_Chr4g0437431 RchiOBHm_Chr4g0437441 RchiOBHm_Chr4g0437491 RchiOBHm_Chr4g0437501 RchiOBHm_Chr4g0437511 RchiOBHm_Chr4g0437521 RchiOBHm_Chr4g0437531 RchiOBHm_Chr4g0437541 RchiOBHm_Chr4g0437551 RchiOBHm_Chr4g0437591 RchiOBHm_Chr4g0437631 RchiOBHm_Chr4g0437641 RchiOBHm_Chr4g0437671 RchiOBHm_Chr4g0437681 RchiOBHm_Chr4g0437711 RchiOBHm_Chr4g0437731 RchiOBHm_Chr4g0437741 RchiOBHm_Chr4g0437751 RchiOBHm_Chr5g0025681 RchiOBHm_Chr5g0025711 RchiOBHm_Chr5g0025731 RchiOBHm_Chr5g0059841 RchiOBHm_Chr6g0272651
rosa_laevigata RLG00000006420 RLG00000006421 RLG00000006423 RLG00000006426 RLG00000006427 RLG00000006428 RLG00000006429 RLG00000006431 RLG00000006433 RLG00000006434 RLG00000006435 RLG00000006436 RLG00000006437 RLG00000006455 RLG00000007746 RLG00000007747 RLG00000007748 RLG00000007749 RLG00000007750 RLG00000009091 RLG00000009092 RLG00000009095 RLG00000009096 RLG00000017856 RLG00000028094 RLG00000029230 RLG00000029231 RLG00000030008 RLG00000032859 RLG00000032860 RLG00000032861 RLG00000032865 RLG00000032868 RLG00000035332
rosa_multiflora Rmu_co7986016.1_g000001 Rmu_co8226539.1_g000001 Rmu_co8349613.1_g000001 Rmu_sc0000272.1_g000014 Rmu_sc0000591.1_g000003 Rmu_sc0000613.1_g000013 Rmu_sc0001629.1_g000022 Rmu_sc0001629.1_g000025 Rmu_sc0002131.1_g000027 Rmu_sc0002137.1_g000006 Rmu_sc0002137.1_g000014 Rmu_sc0002137.1_g000019 Rmu_sc0002472.1_g000002 Rmu_sc0002472.1_g000012 Rmu_sc0002472.1_g000018 Rmu_sc0002472.1_g000034 Rmu_sc0002472.1_g000040 Rmu_sc0002472.1_g000051 Rmu_sc0002638.1_g000005 Rmu_sc0002638.1_g000010 Rmu_sc0002638.1_g000025 Rmu_sc0002638.1_g000030 Rmu_sc0002638.1_g000037 Rmu_sc0002638.1_g000042 Rmu_sc0004339.1_g000019 Rmu_sc0005580.1_g000013 Rmu_sc0006617.1_g000002 Rmu_sc0006986.1_g000003 Rmu_sc0006986.1_g000017 Rmu_sc0008352.1_g000001 Rmu_sc0008352.1_g000002 Rmu_sc0009431.1_g000011 Rmu_sc0011080.1_g000003 Rmu_sc0011080.1_g000006 Rmu_sc0016549.1_g000001 Rmu_sc0016549.1_g000002 Rmu_sc0017154.1_g000001 Rmu_sc0017155.1_g000001 Rmu_sc0028438.1_g000001 Rmu_sc0041413.1_g000001 Rmu_ssc0000330.1_g000022
rosa_roxburghii Rroxscaffold_1G00020750 Rroxscaffold_1G00053460 Rroxscaffold_1G00054250 Rroxscaffold_1G00054260 Rroxscaffold_1G00054270 Rroxscaffold_1G00054290 Rroxscaffold_2G00120210 Rroxscaffold_4G00313460 Rroxscaffold_4G00313470 Rroxscaffold_4G00330630 Rroxscaffold_5G00347070 Rroxscaffold_5G00347110 Rroxscaffold_5G00378670 Rroxscaffold_5G00378690 Rroxscaffold_5G00378720 Rroxscaffold_5G00378730 Rroxscaffold_5G00378740 Rroxscaffold_5G00378760 Rroxscaffold_5G00378780 Rroxscaffold_5G00378790 Rroxscaffold_5G00378800 Rroxscaffold_5G00378810 Rroxscaffold_5G00378820 Rroxscaffold_5G00378840 Rroxscaffold_5G00378860 Rroxscaffold_5G00378870 Rroxscaffold_7G00196060
rosa_rugosa Rorug01G0142200.1 Rorug01G0332800 Rorug02G0012200 Rorug02G0248300 Rorug03G0219000 Rorug03G0219100 Rorug03G0219200 Rorug04G0035100 Rorug04G0301500 Rorug04G0301500 Rorug04G0301700 Rorug04G0301800 Rorug04G0301800 Rorug04G0301900 Rorug04G0302000 Rorug04G0302500 Rorug04G0302600 Rorug04G0302700 Rorug04G0302800 Rorug04G0302900 Rorug04G0303000 Rorug04G0303000 Rorug04G0303100 Rorug04G0303200 Rorug04G0303300 Rorug04G0303400 Rorug04G0303500 Rorug04G0303500 Rorug05G0087800 Rorug05G0088200 Rorug05G0326300 Rorug06G0173000
rosa_samantha Rh2AG222400 Rh4DG224800 Rh4DG359900 Rh4DG360000 Rh4DG360100 Rh4DG360200 Rh4DG360300 Rh4DG360500 Rh4DG360700 Rh4DG360800 Rh4DG360900 Rh4DG361000 Rh5DG177900 Rh5DG178200 Rh5DG178400 Rh6CG187800
rosa_wichuraiana Rw1G013580 Rw4G009020 Rw4G019580 Rw4G030990 Rw4G031000 Rw4G031220 Rw4G031230 Rw4G031240 Rw4G031250 Rw4G031260 Rw4G031270 Rw4G031280 Rw4G031290 Rw4G031310 Rw4G031320 Rw4G031330 Rw4G031340 Rw4G031360 Rw4G031370 Rw5G016310 Rw5G016320 Rw5G016330 Rw5G036760 Rw6G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1088
AccBSI CCGCTC 1 cut(s) 83
AccII CGCG 1 cut(s) 945
AciI CCGC 5 cut(s) 83, 479, 1069, 1371, 1661
AclWI GGATC 1 cut(s) 1675
AcsI RAATTY 3 cut(s) 302, 436, 743
AcuI CTGAAG 1 cut(s) 428
AdeI CACNNNGTG 1 cut(s) 1693
AfaI GTAC 3 cut(s) 8, 396, 504
AfiI CCNNNNNNNGG 3 cut(s) 1326, 1327, 1667
AgsI TTSAA 8 cut(s) 177, 461, 803, 821, 865, 1450, 1565, 1676
AjiI CACGTC 2 cut(s) 1390, 1438
AjnI CCWGG 2 cut(s) 276, 877
AluBI AGCT 8 cut(s) 31, 180, 212, 562, 648, 795, 1196, 1484
AluI AGCT 8 cut(s) 31, 180, 212, 562, 648, 795, 1196, 1484
Alw21I GWGCWC 1 cut(s) 1632
Alw26I GTCTC 4 cut(s) 564, 680, 1296, 1396
Alw44I GTGCAC 1 cut(s) 1628
AlwI GGATC 1 cut(s) 1675
AlwNI CAGNNNCTG 1 cut(s) 1648
Ama87I CYCGRG 2 cut(s) 485, 1618
AoxI GGCC 3 cut(s) 289, 359, 1249
ApaLI GTGCAC 1 cut(s) 1628
ApeKI GCWGC 4 cut(s) 272, 789, 792, 1066
ApoI RAATTY 3 cut(s) 302, 436, 743
AspLEI GCGC 1 cut(s) 625
AspS9I GGNCC 4 cut(s) 290, 540, 1152, 1526
AsuC2I CCSGG 2 cut(s) 1619, 1620
AsuHPI GGTGA 5 cut(s) 605, 616, 745, 748, 1400
AsuII TTCGAA 1 cut(s) 153
AvaI CYCGRG 2 cut(s) 485, 1618
AvaII GGWCC 3 cut(s) 540, 1152, 1526
BaeGI GKGCMC 1 cut(s) 1632
BauI CACGAG 1 cut(s) 1058
Bbv12I GWGCWC 1 cut(s) 1632
BbvI GCAGC 4 cut(s) 259, 801, 804, 1078
BccI CCATC 4 cut(s) 262, 577, 1241, 1264
BceAI ACGGC 1 cut(s) 999
BcgI CGANNNNNNTGC 4 cut(s) 1534, 1568, 1573, 1607
BciT130I CCWGG 2 cut(s) 278, 879
BclI TGATCA 2 cut(s) 471, 1278
BcnI CCSGG 2 cut(s) 1619, 1620
BcoDI GTCTC 4 cut(s) 564, 680, 1296, 1396
BfaI CTAG 7 cut(s) 197, 363, 774, 1197, 1211, 1326, 1352
BfmI CTRYAG 2 cut(s) 390, 1600
BglII AGATCT 1 cut(s) 423
BisI GCNGC 4 cut(s) 273, 790, 793, 1067
BlsI GCNGC 4 cut(s) 274, 791, 794, 1068
Bme1390I CCNGG 4 cut(s) 278, 879, 1619, 1620
Bme18I GGWCC 3 cut(s) 540, 1152, 1526
BmeT110I CYCGRG 2 cut(s) 485, 1618
BmgBI CACGTC 2 cut(s) 1390, 1438
BmgT120I GGNCC 4 cut(s) 290, 540, 1152, 1526
BmiI GGNNCC 4 cut(s) 483, 542, 875, 1527
BmrFI CCNGG 4 cut(s) 278, 879, 1619, 1620
BmrI ACTGGG 1 cut(s) 1685
BmsI GCATC 3 cut(s) 634, 891, 1042
BmuI ACTGGG 1 cut(s) 1685
BplI GAGNNNNNCTC 4 cut(s) 824, 856, 1448, 1480
BpmI CTGGAG 1 cut(s) 1014
Bpu14I TTCGAA 1 cut(s) 153
BpuMI CCSGG 2 cut(s) 1619, 1620
BsaJI CCNNGG 9 cut(s) 276, 277, 286, 310, 544, 637, 877, 1617, 1618
BsaWI WCCGGW 1 cut(s) 1453
BsaXI ACNNNNNCTCC 6 cut(s) 124, 154, 858, 888, 1662, 1692
Bsc4I CCNNNNNNNGG 3 cut(s) 1326, 1327, 1667
Bse1I ACTGG 3 cut(s) 903, 997, 1691
Bse3DI GCAATG 2 cut(s) 474, 993
BseBI CCWGG 2 cut(s) 278, 879
BseDI CCNNGG 9 cut(s) 276, 277, 286, 310, 544, 637, 877, 1617, 1618
BseGI GGATG 2 cut(s) 19, 273
BseLI CCNNNNNNNGG 3 cut(s) 1326, 1327, 1667
BseMI GCAATG 2 cut(s) 474, 993
BseMII CTCAG 5 cut(s) 675, 774, 883, 1257, 1479
BseNI ACTGG 3 cut(s) 903, 997, 1691
BseSI GKGCMC 1 cut(s) 1632
BseXI GCAGC 4 cut(s) 259, 801, 804, 1078
Bsh1236I CGCG 1 cut(s) 945
Bsh1285I CGRYCG 1 cut(s) 87
BshFI GGCC 3 cut(s) 291, 361, 1251
BsiEI CGRYCG 1 cut(s) 87
BsiHKAI GWGCWC 1 cut(s) 1632
BsiHKCI CYCGRG 2 cut(s) 485, 1618
BsiSI CCGG 2 cut(s) 1454, 1619
BslFI GGGAC 3 cut(s) 526, 1303, 1539
BslI CCNNNNNNNGG 3 cut(s) 1326, 1327, 1667
BsmAI GTCTC 4 cut(s) 564, 680, 1296, 1396
BsmBI CGTCTC 1 cut(s) 1396
BsmFI GGGAC 3 cut(s) 526, 1303, 1539
BsmI GAATGC 1 cut(s) 1505
BsnI GGCC 3 cut(s) 291, 361, 1251
BsoBI CYCGRG 2 cut(s) 485, 1618
Bsp119I TTCGAA 1 cut(s) 153
Bsp1286I GDGCHC 1 cut(s) 1632
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 4 cut(s) 423, 471, 1278, 1680
Bsp19I CCATGG 3 cut(s) 286, 310, 637
BspACI CCGC 5 cut(s) 83, 479, 1069, 1371, 1661
BspANI GGCC 3 cut(s) 291, 361, 1251
BspCNI CTCAG 5 cut(s) 676, 773, 882, 1256, 1478
BspFNI CGCG 1 cut(s) 945
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 4 cut(s) 483, 542, 875, 1527
BspMAI CTGCAG 1 cut(s) 394
BspPI GGATC 1 cut(s) 1675
BspQI GCTCTTC 1 cut(s) 1176
BspT104I TTCGAA 1 cut(s) 153
BsrBI CCGCTC 1 cut(s) 83
BsrDI GCAATG 2 cut(s) 474, 993
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 3 cut(s) 903, 997, 1691
BssECI CCNNGG 9 cut(s) 276, 277, 286, 310, 544, 637, 877, 1617, 1618
BssMI GATC 4 cut(s) 423, 471, 1278, 1680
BssSI CACGAG 1 cut(s) 1058
BssT1I CCWWGG 4 cut(s) 286, 310, 544, 637
Bst2BI CACGAG 1 cut(s) 1058
Bst2UI CCWGG 2 cut(s) 278, 879
Bst4CI ACNGT 4 cut(s) 1007, 1156, 1604, 1654
Bst6I CTCTTC 1 cut(s) 1176
BstAPI GCANNNNNTGC 1 cut(s) 1574
BstAUI TGTACA 1 cut(s) 6
BstBI TTCGAA 1 cut(s) 153
BstC8I GCNNGC 3 cut(s) 359, 1146, 1661
BstDEI CTNAG 7 cut(s) 611, 684, 760, 869, 1243, 1295, 1465
BstDSI CCRYGG 3 cut(s) 286, 310, 637
BstF5I GGATG 2 cut(s) 19, 273
BstFNI CGCG 1 cut(s) 945
BstHHI GCGC 1 cut(s) 625
BstKTI GATC 4 cut(s) 426, 474, 1281, 1683
BstMAI GTCTC 4 cut(s) 564, 680, 1296, 1396
BstMBI GATC 4 cut(s) 423, 471, 1278, 1680
BstMCI CGRYCG 1 cut(s) 87
BstMWI GCNNNNNNNGC 2 cut(s) 629, 1574
BstNI CCWGG 2 cut(s) 278, 879
BstSCI CCNGG 4 cut(s) 276, 877, 1617, 1618
BstSFI CTRYAG 2 cut(s) 390, 1600
BstSLI GKGCMC 1 cut(s) 1632
BstUI CGCG 1 cut(s) 945
BstV1I GCAGC 4 cut(s) 259, 801, 804, 1078
BstX2I RGATCY 1 cut(s) 423
BstYI RGATCY 1 cut(s) 423
BsuRI GGCC 3 cut(s) 291, 361, 1251
BtgI CCRYGG 3 cut(s) 286, 310, 637
BtrI CACGTC 2 cut(s) 1390, 1438
BtsCI GGATG 2 cut(s) 19, 273
BtsIMutI CAGTG 4 cut(s) 452, 910, 1083, 1698
Cac8I GCNNGC 3 cut(s) 359, 1146, 1661
CaiI CAGNNNCTG 1 cut(s) 1648
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 1 cut(s) 625
Cfr13I GGNCC 4 cut(s) 290, 540, 1152, 1526
Cfr9I CCCGGG 1 cut(s) 1618
Csp6I GTAC 3 cut(s) 7, 395, 503
CviQI GTAC 3 cut(s) 7, 395, 503
DdeI CTNAG 7 cut(s) 611, 684, 760, 869, 1243, 1295, 1465
DpnI GATC 4 cut(s) 425, 473, 1280, 1682
DpnII GATC 4 cut(s) 423, 471, 1278, 1680
DraI TTTAAA 1 cut(s) 1038
DraIII CACNNNGTG 1 cut(s) 1693
DrdI GACNNNNNNGTC 1 cut(s) 1088
DseDI GACNNNNNNGTC 1 cut(s) 1088
Eam1104I CTCTTC 1 cut(s) 1176
EarI CTCTTC 1 cut(s) 1176
Eco130I CCWWGG 4 cut(s) 286, 310, 544, 637
Eco147I AGGCCT 1 cut(s) 361
Eco47I GGWCC 3 cut(s) 540, 1152, 1526
Eco57I CTGAAG 1 cut(s) 428
Eco88I CYCGRG 2 cut(s) 485, 1618
EcoO109I RGGNCCY 2 cut(s) 540, 1526
EcoRII CCWGG 2 cut(s) 276, 877
EcoT14I CCWWGG 4 cut(s) 286, 310, 544, 637
EcoT22I ATGCAT 1 cut(s) 415
ErhI CCWWGG 4 cut(s) 286, 310, 544, 637
Esp3I CGTCTC 1 cut(s) 1396
FaqI GGGAC 3 cut(s) 526, 1303, 1539
FauI CCCGC 2 cut(s) 1378, 1668
FbaI TGATCA 2 cut(s) 471, 1278
Fnu4HI GCNGC 4 cut(s) 273, 790, 793, 1067
FokI GGATG 2 cut(s) 6, 280
Fsp4HI GCNGC 4 cut(s) 273, 790, 793, 1067
FspBI CTAG 7 cut(s) 197, 363, 774, 1197, 1211, 1326, 1352
GlaI GCGC 1 cut(s) 624
GluI GCNGC 4 cut(s) 273, 790, 793, 1067
GsuI CTGGAG 1 cut(s) 1014
HaeIII GGCC 3 cut(s) 291, 361, 1251
HapII CCGG 2 cut(s) 1454, 1619
HhaI GCGC 1 cut(s) 625
Hin6I GCGC 1 cut(s) 623
HinP1I GCGC 1 cut(s) 623
HincII GTYRAC 1 cut(s) 837
HindII GTYRAC 1 cut(s) 837
HindIII AAGCTT 1 cut(s) 178
HinfI GANTC 9 cut(s) 150, 244, 262, 282, 661, 1289, 1331, 1461, 1588
HpaI GTTAAC 1 cut(s) 837
HpaII CCGG 2 cut(s) 1454, 1619
HphI GGTGA 5 cut(s) 605, 616, 745, 748, 1400
Hpy166II GTNNAC 4 cut(s) 243, 837, 1081, 1630
Hpy188III TCNNGA 4 cut(s) 88, 665, 931, 1049
Hpy8I GTNNAC 4 cut(s) 243, 837, 1081, 1630
HpyAV CCTTC 7 cut(s) 303, 584, 643, 710, 797, 928, 1463
HpyCH4III ACNGT 4 cut(s) 1007, 1156, 1604, 1654
HpyCH4IV ACGT 3 cut(s) 1106, 1389, 1437
HpyCH4V TGCA 8 cut(s) 392, 413, 495, 1101, 1387, 1441, 1594, 1630
HpyF10VI GCNNNNNNNGC 2 cut(s) 629, 1574
HpyF3I CTNAG 7 cut(s) 611, 684, 760, 869, 1243, 1295, 1465
HpySE526I ACGT 3 cut(s) 1106, 1389, 1437
HspAI GCGC 1 cut(s) 623
Ksp22I TGATCA 2 cut(s) 471, 1278
KspAI GTTAAC 1 cut(s) 837
Kzo9I GATC 4 cut(s) 423, 471, 1278, 1680
LguI GCTCTTC 1 cut(s) 1176
LmnI GCTCC 3 cut(s) 333, 417, 879
Lsp1109I GCAGC 4 cut(s) 259, 801, 804, 1078
LweI GCATC 3 cut(s) 634, 891, 1042
MaeI CTAG 7 cut(s) 197, 363, 774, 1197, 1211, 1326, 1352
MaeII ACGT 3 cut(s) 1106, 1389, 1437
MalI GATC 4 cut(s) 425, 473, 1280, 1682
MbiI CCGCTC 1 cut(s) 83
MboI GATC 4 cut(s) 423, 471, 1278, 1680
MboII GAAGA 5 cut(s) 434, 445, 752, 1154, 1163
MflI RGATCY 1 cut(s) 423
MhlI GDGCHC 1 cut(s) 1632
MluCI AATT 6 cut(s) 165, 201, 302, 436, 743, 1214
MlyI GAGTC 3 cut(s) 238, 291, 1298
Mph1103I ATGCAT 1 cut(s) 415
MseI TTAA 5 cut(s) 371, 650, 836, 845, 1037
MslI CAYNNNNRTG 4 cut(s) 408, 581, 887, 1050
MspA1I CMGCKG 2 cut(s) 1069, 1373
MspI CCGG 2 cut(s) 1454, 1619
MspR9I CCNGG 4 cut(s) 278, 879, 1619, 1620
Mva1269I GAATGC 1 cut(s) 1505
MvaI CCWGG 2 cut(s) 278, 879
MvnI CGCG 1 cut(s) 945
MwoI GCNNNNNNNGC 2 cut(s) 629, 1574
NciI CCSGG 2 cut(s) 1619, 1620
NcoI CCATGG 3 cut(s) 286, 310, 637
NdeII GATC 4 cut(s) 423, 471, 1278, 1680
NlaIV GGNNCC 4 cut(s) 483, 542, 875, 1527
NsiI ATGCAT 1 cut(s) 415
NspV TTCGAA 1 cut(s) 153
PagI TCATGA 1 cut(s) 1048
PasI CCCWGGG 1 cut(s) 277
PceI AGGCCT 1 cut(s) 361
PciSI GCTCTTC 1 cut(s) 1176
PcsI WCGNNNNNNNCGW 1 cut(s) 1089
PctI GAATGC 1 cut(s) 1505
PfeI GAWTC 6 cut(s) 150, 262, 661, 1331, 1461, 1588
PkrI GCNGC 4 cut(s) 274, 791, 794, 1068
PleI GAGTC 3 cut(s) 238, 290, 1297
PpsI GAGTC 3 cut(s) 238, 290, 1297
PpuMI RGGWCCY 2 cut(s) 540, 1526
Psp5II RGGWCCY 2 cut(s) 540, 1526
Psp6I CCWGG 2 cut(s) 276, 877
PspGI CCWGG 2 cut(s) 276, 877
PspN4I GGNNCC 4 cut(s) 483, 542, 875, 1527
PspPI GGNCC 4 cut(s) 290, 540, 1152, 1526
PspPPI RGGWCCY 2 cut(s) 540, 1526
PstI CTGCAG 1 cut(s) 394
PstNI CAGNNNCTG 1 cut(s) 1648
PsuI RGATCY 1 cut(s) 423
RsaI GTAC 3 cut(s) 8, 396, 504
RsaNI GTAC 3 cut(s) 7, 395, 503
RseI CAYNNNNRTG 4 cut(s) 408, 581, 887, 1050
SapI GCTCTTC 1 cut(s) 1176
SaqAI TTAA 5 cut(s) 371, 650, 836, 845, 1037
SatI GCNGC 4 cut(s) 273, 790, 793, 1067
Sau3AI GATC 4 cut(s) 423, 471, 1278, 1680
Sau96I GGNCC 4 cut(s) 290, 540, 1152, 1526
SchI GAGTC 3 cut(s) 238, 291, 1298
ScrFI CCNGG 4 cut(s) 278, 879, 1619, 1620
SduI GDGCHC 1 cut(s) 1632
SfaNI GCATC 3 cut(s) 634, 891, 1042
SfcI CTRYAG 2 cut(s) 390, 1600
SfuI TTCGAA 1 cut(s) 153
SinI GGWCC 3 cut(s) 540, 1152, 1526
SmaI CCCGGG 1 cut(s) 1620
SmiMI CAYNNNNRTG 4 cut(s) 408, 581, 887, 1050
Sse9I AATT 6 cut(s) 165, 201, 302, 436, 743, 1214
SseBI AGGCCT 1 cut(s) 361
SsiI CCGC 5 cut(s) 83, 479, 1069, 1371, 1661
SspI AATATT 2 cut(s) 849, 1424
SspMI CTAG 7 cut(s) 197, 363, 774, 1197, 1211, 1326, 1352
StuI AGGCCT 1 cut(s) 361
StyD4I CCNGG 4 cut(s) 276, 877, 1617, 1618
StyI CCWWGG 4 cut(s) 286, 310, 544, 637
TaaI ACNGT 4 cut(s) 1007, 1156, 1604, 1654
TaiI ACGT 3 cut(s) 1109, 1392, 1440
TaqI TCGA 7 cut(s) 87, 153, 581, 930, 1092, 1531, 1583
TasI AATT 6 cut(s) 165, 201, 302, 436, 743, 1214
TatI WGTACW 2 cut(s) 6, 394
TfiI GAWTC 6 cut(s) 150, 262, 661, 1331, 1461, 1588
Tru1I TTAA 5 cut(s) 371, 650, 836, 845, 1037
Tru9I TTAA 5 cut(s) 371, 650, 836, 845, 1037
TscAI CASTG 4 cut(s) 452, 910, 1083, 1698
TseI GCWGC 4 cut(s) 272, 789, 792, 1066
TspDTI ATGAA 9 cut(s) 353, 398, 729, 753, 1163, 1245, 1428, 1498, 1562
TspMI CCCGGG 1 cut(s) 1618
TspRI CASTG 4 cut(s) 452, 910, 1083, 1698
VneI GTGCAC 1 cut(s) 1628
VpaK11BI GGWCC 3 cut(s) 540, 1152, 1526
XapI RAATTY 3 cut(s) 302, 436, 743
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
XmaI CCCGGG 1 cut(s) 1618
XspI CTAG 7 cut(s) 197, 363, 774, 1197, 1211, 1326, 1352
Zsp2I ATGCAT 1 cut(s) 415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.