RLG00000035332

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0000078
Physical Location & Seq
Forward (+)
65089103 .. 65093714
4612 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035332

Sequence Viewer

Length: 2781 bp
ATGGCTTCTTCTAAGCAGGTTCAGGTCACAGCTTGTAATCTATGGCTTCTGTTCCTCACACTTCCATGTTCTTCTTCCACTGAATCACTCTATCAGCATTCTTTGGTTGTTGGTGAAAATGCTACTTTAGGATTGTCTCATGTGAAGGTGAAGATATCAACAACATCACGATTTCAAACTTTTGAGGTTTGTTTCCCTAGAAATACGTCCCGTGGGATAGGAATGTGCCCTGAGAGCCAGTGGGAGAAAGTTTCCAAGGGGTCCTGGTCTGGATCCATGTCACCTTTTGAGCACAAACTCTTAGATATGCTAACAGCTGGTTCATCCTTACAGAATTTTGAAGTGTTCATTAGAGAAGAATTTTTCTTATCTCGCTTAATATTTTTTGTATCTGGCATAGTCCTGATGACCTTGGCGCCTTATCTGAGTAACGCATTTGTGTTTTATTACATCAGCGGAAAGGTAATAGGGGTGGTTCTTGTAATATTGATTTTCCTTCTTCAGGTAATGAAGCTTCTCCCAACTGGTCGGAAGAATTCACTTGCAATTTTCGTCTTGTCATCTGTGGTTGGTTTGGGATCTTTTCTCCTCAGCTACATCCCAGGACTACTAAGTTCAGTACTCACAGAGCTTGGAATTAGTGAGGATATGTATAATGCTTTGGCCATATTTCTTCTGGCTTTTGTTTTTCTGGCTGGAGCATGGTTGGGATTCTGGACAGTTCGTAAAATGGCCTTGAACAAAGATAGATCAATTGATTCTAGAACATCTCTGTTTGTTTTGTGGACCATGCGCTTCGTAGCTGTTATTTTTCTTTCACAGAGTTCCAGCGATCAACTACTGGCAGCAGGAGCTATAATGCAAGGGAAATCTTTAGATGGATCGATCCGTGGTCGTCACATGTACAGGAAATTGTTGAGATCACCCAAAAAGAAGAGCAGAATATTAGAGATTCCTGATTCTTCACCTTCTGAATGTGCTCCTAATGAAGTCGTTAACGAGTTTCAGAGCAACGAGGACTATAAGCTACCTAGGCCTGGTTCCAGAAACTCTACCTCGGCTTCACGCATTTCACCTGGCCTAGGTTCTAAAAGTGCATCACCATCAGATGGAAGGTTATATCCTTCTTACATCCACACTACACCTGAAAGGAGACCAATGACAAAACATGAGTGGGACAGTCTCACTAGACACACAACAGAGAAGGCCTTGGAAGAACTTGCTTCTTCTCCTGATTTTGGCAAATGGGTTGTTGCAAATGCAGATAGAATCAGCGTAACCCCCCTTAGTTTGCGAGCTCTGATCAAGGCCTTTCACAAACTATGGTGGACTCCGACTCGCATTCAGAATCTGATGCGTCTGCAGGGAATCAAAGGTCCTTCTTACAGACTTCACTATGGAAACACCAAAGAAATCATGAACATGCAAAATGAAGCGCTGAGCAGGCCCATAAATGTATCACATGATATCTATTCTCATGTTCAACCTCATATTCATGAATGGACCAAGAGATATGGGAAAAATTTTCTTCAATGGTATGGTCTTCAACCTGTTTTGGTTGTTACAGAAGCTGAATTGTGCAAAGAGATCCTCAACAACAGAGATGGAGTTTATCCGAAACAGAAGCCACCTGAAGTTGTGAAGAAGCTATTAGGAGATAGCATATCCATGACAGAAGGTGAAAAATGGGTGAAGTTGAGGAAGGTGTCTAATCATGCCTTCCATGGAGACAGCTTAAAAAATATGTTTGCAGACATGAAAGCTAGTGCTGAGACGATGGTAGAAAGTTGGAAAGTTCATGAAGGAAAAGAGATTGATGTGTATGAAGAATTTCGGTTGTACACTTCTGAAGTGATTTCAAGGACAGCATTTGGCAGCAGCTACTTAGAAGGCAAGACCATTTTTGACAAGTTGTCGAAGTTATACGGCATAATATTCAGAAATCTTCTCAAAATCAGGATTCCTGGCATGAGCAAGGTTTTTAGAACCAGTGATGACATTGAATCAGACGAGCTGGAGAAAGGAATACGCGACTCCATATTGGAAATTGTTAAGAAAAGAGAAATGAAGGCTACCATTGGAGAAGACAACAGCTTTGGGAATGATTTTCTAGGATTACTTTTAGAGGCTCACCATGGTTCCAATGACAAGCAGCGGATTTCGATTGACGAGTTGGTTGAGGAATGCAGGACTTTTTACTTTGCTGGACAAGAGACTACTAATTCTATACTTTCATGGATTATCCTTCTTCTGGCACAGCACCCTGATTGGCAAGAGGAAGCAAGAAAAGAGGTGCTACAGTTGTTTGGCAAGCAGACTCCAAATTACGATGGCCTTGCCAAACTCAAAACAATGAGTATGATTATCAATGAGACTCTAAGGTTATATTCTCCTGCTCATTTGCTTGTAAGGAAAGTAGCAAGGGAAGTCAGACTGGGAAAACTCCTAGTTCCTGCTAATATGGAGTTGCTTGTCCCACCTCTAGCACTTCACCATGAAACTCAGCTCTGGGGACAAGATGTGGGATTGTTCAAGCCAGAGCGATTCTCTGAAGGGGTTGCTAAGGCTACAAACGATAACATGGCCGCATTCATGCCCTTTGGAATGGGACCTAGAATGTGTGTCGGATTCAACTTTGCTGCCACTGAAGCAAAGATTGCTCTGTCAATGATTCTCCAACGCTTCTCATTTACCCTTTCCCCGGGTTATGTCCACTCACCTTGTCCGTCTCTTACCGTTCGACCACAACATGGAGTTCAAGTAATACTACACTCACTCTGA

Protein Analysis

927

Amino Acids

104.44

Weight (kDa)

9.17

Isoelectric Point (pI)

41.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NEMP PF10225 117 - 287 1.3e-30 NEMP family
p450 PF00067 495 - 909 2.2e-80 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000078)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g15240 FvH4_3g15270 FvH4_3g15280 FvH4_3g32750 FvH4_3g32760 FvH4_3g32760 FvH4_3g32880 FvH4_4g08190 FvH4_4g29160 FvH4_4g29370 FvH4_4g29400 FvH4_4g29420 FvH4_4g29420 FvH4_4g29420 FvH4_4g29430 FvH4_4g29440 FvH4_4g29450 FvH4_4g29450 FvH4_4g29450 FvH4_4g29460 FvH4_4g29461 FvH4_4g29462 FvH4_4g29463 FvH4_4g29480 FvH4_4g29481 FvH4_4g29482 FvH4_4g29483 FvH4_4g29484 FvH4_6g26720 FvH4_6g26720
malus_domestica MD00G1065200.v1.1 MD06G1145800.v1.1 MD06G1146000.v1.1 MD06G1146700.v1.1 MD13G1031000.v1.1 MD13G1033900.v1.1 MD13G1057000.v1.1 MD13G1057100.v1.1 MD13G1057200.v1.1 MD13G1057400.v1.1 MD13G1057800.v1.1 MD13G1058000.v1.1 MD13G1058100.v1.1 MD13G1058200.v1.1 MD13G1058300.v1.1 MD13G1058500.v1.1 MD13G1058600.v1.1 MD13G1058700.v1.1 MD16G1035700.v1.1 MD16G1056400.v1.1 MD16G1056600.v1.1 MD16G1056700.v1.1 MD16G1056900.v1.1 MD16G1058100.v1.1
prunus_persica Prupe.1G291100_v2.0.a1 Prupe.1G291200_v2.0.a1 Prupe.1G291500_v2.0.a1 Prupe.1G291600_v2.0.a1 Prupe.1G291700_v2.0.a1 Prupe.1G291800_v2.0.a1 Prupe.1G291900_v2.0.a1 Prupe.1G292000_v2.0.a1 Prupe.1G292100_v2.0.a1 Prupe.1G292400_v2.0.a1 Prupe.1G292500_v2.0.a1 Prupe.1G292600_v2.0.a1 Prupe.1G292700_v2.0.a1 Prupe.1G292800_v2.0.a1 Prupe.1G296000_v2.0.a1 Prupe.1G296100_v2.0.a1 Prupe.1G296300_v2.0.a1 Prupe.1G296600_v2.0.a1 Prupe.1G296900_v2.0.a1 Prupe.1G534600_v2.0.a1 Prupe.5G046700_v2.0.a1 Prupe.8G021800_v2.0.a1 Prupe.8G026400_v2.0.a1
pyrus_communis pycom06g13510 pycom06g13530 pycom06g13540 pycom06g13570 pycom06g13590 pycom06g13620 pycom13g02710 pycom13g02930 pycom13g05100 pycom13g05110 pycom13g05140 pycom13g05160 pycom13g05170 pycom13g05190 pycom13g05200 pycom13g05210 pycom13g05220 pycom16g03060 pycom16g04940 pycom16g04960 pycom16g04990 pycom16g05000 pycom16g05080
rosa_chinensis RchiOBHm_Chr1g0337981 RchiOBHm_Chr1g0337991 RchiOBHm_Chr1g0339431 RchiOBHm_Chr4g0402401 RchiOBHm_Chr4g0420571 RchiOBHm_Chr4g0420601 RchiOBHm_Chr4g0420611 RchiOBHm_Chr4g0420621 RchiOBHm_Chr4g0420631 RchiOBHm_Chr4g0437211 RchiOBHm_Chr4g0437221 RchiOBHm_Chr4g0437251 RchiOBHm_Chr4g0437431 RchiOBHm_Chr4g0437441 RchiOBHm_Chr4g0437491 RchiOBHm_Chr4g0437501 RchiOBHm_Chr4g0437511 RchiOBHm_Chr4g0437521 RchiOBHm_Chr4g0437531 RchiOBHm_Chr4g0437541 RchiOBHm_Chr4g0437551 RchiOBHm_Chr4g0437591 RchiOBHm_Chr4g0437631 RchiOBHm_Chr4g0437641 RchiOBHm_Chr4g0437671 RchiOBHm_Chr4g0437681 RchiOBHm_Chr4g0437711 RchiOBHm_Chr4g0437731 RchiOBHm_Chr4g0437741 RchiOBHm_Chr4g0437751 RchiOBHm_Chr5g0025681 RchiOBHm_Chr5g0025711 RchiOBHm_Chr5g0025731 RchiOBHm_Chr5g0059841 RchiOBHm_Chr6g0272651
rosa_laevigata RLG00000006420 RLG00000006421 RLG00000006423 RLG00000006426 RLG00000006427 RLG00000006428 RLG00000006429 RLG00000006431 RLG00000006433 RLG00000006434 RLG00000006435 RLG00000006436 RLG00000006437 RLG00000006455 RLG00000007746 RLG00000007747 RLG00000007748 RLG00000007749 RLG00000007750 RLG00000009091 RLG00000009092 RLG00000009095 RLG00000009096 RLG00000017856 RLG00000028094 RLG00000029230 RLG00000029231 RLG00000030008 RLG00000032859 RLG00000032860 RLG00000032861 RLG00000032865 RLG00000032868 RLG00000035332
rosa_multiflora Rmu_co7986016.1_g000001 Rmu_co8226539.1_g000001 Rmu_co8349613.1_g000001 Rmu_sc0000272.1_g000014 Rmu_sc0000591.1_g000003 Rmu_sc0000613.1_g000013 Rmu_sc0001629.1_g000022 Rmu_sc0001629.1_g000025 Rmu_sc0002131.1_g000027 Rmu_sc0002137.1_g000006 Rmu_sc0002137.1_g000014 Rmu_sc0002137.1_g000019 Rmu_sc0002472.1_g000002 Rmu_sc0002472.1_g000012 Rmu_sc0002472.1_g000018 Rmu_sc0002472.1_g000034 Rmu_sc0002472.1_g000040 Rmu_sc0002472.1_g000051 Rmu_sc0002638.1_g000005 Rmu_sc0002638.1_g000010 Rmu_sc0002638.1_g000025 Rmu_sc0002638.1_g000030 Rmu_sc0002638.1_g000037 Rmu_sc0002638.1_g000042 Rmu_sc0004339.1_g000019 Rmu_sc0005580.1_g000013 Rmu_sc0006617.1_g000002 Rmu_sc0006986.1_g000003 Rmu_sc0006986.1_g000017 Rmu_sc0008352.1_g000001 Rmu_sc0008352.1_g000002 Rmu_sc0009431.1_g000011 Rmu_sc0011080.1_g000003 Rmu_sc0011080.1_g000006 Rmu_sc0016549.1_g000001 Rmu_sc0016549.1_g000002 Rmu_sc0017154.1_g000001 Rmu_sc0017155.1_g000001 Rmu_sc0028438.1_g000001 Rmu_sc0041413.1_g000001 Rmu_ssc0000330.1_g000022
rosa_roxburghii Rroxscaffold_1G00020750 Rroxscaffold_1G00053460 Rroxscaffold_1G00054250 Rroxscaffold_1G00054260 Rroxscaffold_1G00054270 Rroxscaffold_1G00054290 Rroxscaffold_2G00120210 Rroxscaffold_4G00313460 Rroxscaffold_4G00313470 Rroxscaffold_4G00330630 Rroxscaffold_5G00347070 Rroxscaffold_5G00347110 Rroxscaffold_5G00378670 Rroxscaffold_5G00378690 Rroxscaffold_5G00378720 Rroxscaffold_5G00378730 Rroxscaffold_5G00378740 Rroxscaffold_5G00378760 Rroxscaffold_5G00378780 Rroxscaffold_5G00378790 Rroxscaffold_5G00378800 Rroxscaffold_5G00378810 Rroxscaffold_5G00378820 Rroxscaffold_5G00378840 Rroxscaffold_5G00378860 Rroxscaffold_5G00378870 Rroxscaffold_7G00196060
rosa_rugosa Rorug01G0142200.1 Rorug01G0332800 Rorug02G0012200 Rorug02G0248300 Rorug03G0219000 Rorug03G0219100 Rorug03G0219200 Rorug04G0035100 Rorug04G0301500 Rorug04G0301500 Rorug04G0301700 Rorug04G0301800 Rorug04G0301800 Rorug04G0301900 Rorug04G0302000 Rorug04G0302500 Rorug04G0302600 Rorug04G0302700 Rorug04G0302800 Rorug04G0302900 Rorug04G0303000 Rorug04G0303000 Rorug04G0303100 Rorug04G0303200 Rorug04G0303300 Rorug04G0303400 Rorug04G0303500 Rorug04G0303500 Rorug05G0087800 Rorug05G0088200 Rorug05G0326300 Rorug06G0173000
rosa_samantha Rh2AG222400 Rh4DG224800 Rh4DG359900 Rh4DG360000 Rh4DG360100 Rh4DG360200 Rh4DG360300 Rh4DG360500 Rh4DG360700 Rh4DG360800 Rh4DG360900 Rh4DG361000 Rh5DG177900 Rh5DG178200 Rh5DG178400 Rh6CG187800
rosa_wichuraiana Rw1G013580 Rw4G009020 Rw4G019580 Rw4G030990 Rw4G031000 Rw4G031220 Rw4G031230 Rw4G031240 Rw4G031250 Rw4G031260 Rw4G031270 Rw4G031280 Rw4G031290 Rw4G031310 Rw4G031320 Rw4G031330 Rw4G031340 Rw4G031360 Rw4G031370 Rw5G016310 Rw5G016320 Rw5G016330 Rw5G036760 Rw6G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 7
AccB1I GGYRCC 1 cut(s) 415
AccB7I CCANNNNNTGG 2 cut(s) 1109, 2750
AccII CGCG 1 cut(s) 2031
AciI CCGC 3 cut(s) 456, 2155, 2586
AclWI GGATC 6 cut(s) 267, 280, 586, 880, 889, 1582
AcoI YGGCCR 2 cut(s) 663, 2583
AcsI RAATTY 5 cut(s) 334, 359, 535, 1522, 1829
AcuI CTGAAG 5 cut(s) 485, 1653, 1869, 2571, 2667
AcyI GRCGYC 1 cut(s) 416
AfaI GTAC 3 cut(s) 621, 905, 1841
AfeI AGCGCT 1 cut(s) 1437
AfiI CCNNNNNNNGG 8 cut(s) 502, 1037, 1082, 1109, 1238, 2251, 2701, 2750
AflIII ACRYGT 1 cut(s) 900
AhdI GACNNNNNGTC 1 cut(s) 1912
AjnI CCWGG 5 cut(s) 263, 601, 1036, 1075, 1963
Alw21I GWGCWC 3 cut(s) 294, 982, 1300
Alw26I GTCTC 8 cut(s) 141, 1147, 1187, 1722, 1766, 2207, 2366, 2733
AlwI GGATC 6 cut(s) 267, 280, 586, 880, 889, 1582
AlwNI CAGNNNCTG 2 cut(s) 1351, 1571
Ama87I CYCGRG 1 cut(s) 2701
Aor51HI AGCGCT 1 cut(s) 1437
AoxI GGCC 9 cut(s) 663, 732, 1034, 1078, 1206, 1308, 1445, 2332, 2583
ApeKI GCWGC 5 cut(s) 845, 1875, 1878, 2152, 2639
ApoI RAATTY 5 cut(s) 334, 359, 535, 1522, 1829
ArsI GACNNNNNNTTYG 2 cut(s) 2078, 2110
Asp700I GAANNNNTTC 1 cut(s) 1830
AspA2I CCTAGG 2 cut(s) 1031, 1081
AspLEI GCGC 3 cut(s) 418, 795, 1438
AspS9I GGNCC 6 cut(s) 261, 786, 1376, 1446, 1503, 2609
AsuC2I CCSGG 2 cut(s) 2702, 2703
AvaI CYCGRG 1 cut(s) 2701
AvaII GGWCC 5 cut(s) 261, 786, 1376, 1503, 2609
AvrII CCTAGG 2 cut(s) 1031, 1081
BaeGI GKGCMC 1 cut(s) 230
BalI TGGCCA 1 cut(s) 665
BamHI GGATCC 1 cut(s) 272
BanI GGYRCC 1 cut(s) 415
BanII GRGCYC 1 cut(s) 1300
BarI GAAGNNNNNNTAC 2 cut(s) 498, 530
BbsI GAAGAC 2 cut(s) 1535, 2091
Bbv12I GWGCWC 3 cut(s) 294, 982, 1300
BbvCI CCTCAGC 1 cut(s) 590
BbvI GCAGC 5 cut(s) 857, 1887, 1890, 2164, 2626
BccI CCATC 6 cut(s) 872, 1103, 1111, 1598, 1771, 2324
BceAI ACGGC 1 cut(s) 1942
BcgI CGANNNNNNTGC 2 cut(s) 2318, 2352
BciT130I CCWGG 5 cut(s) 265, 603, 1038, 1077, 1965
BclI TGATCA 1 cut(s) 1302
BcnI CCSGG 2 cut(s) 2702, 2703
BcoDI GTCTC 8 cut(s) 141, 1147, 1187, 1722, 1766, 2207, 2366, 2733
BfmI CTRYAG 2 cut(s) 1361, 2297
BfoI RGCGCY 2 cut(s) 419, 1439
BfuAI ACCTGC 1 cut(s) 7
BisI GCNGC 6 cut(s) 846, 1876, 1879, 2153, 2586, 2640
BlnI CCTAGG 2 cut(s) 1031, 1081
BlpI GCTNAGC 1 cut(s) 1439
BlsI GCNGC 6 cut(s) 847, 1877, 1880, 2154, 2587, 2641
BmcAI AGTACT 1 cut(s) 621
Bme1390I CCNGG 7 cut(s) 265, 603, 1038, 1077, 1965, 2702, 2703
Bme18I GGWCC 5 cut(s) 261, 786, 1376, 1503, 2609
BmeRI GACNNNNNGTC 1 cut(s) 1912
BmeT110I CYCGRG 1 cut(s) 2701
BmgT120I GGNCC 6 cut(s) 261, 786, 1376, 1446, 1503, 2609
BmiI GGNNCC 6 cut(s) 262, 274, 417, 1042, 2140, 2610
BmrFI CCNGG 7 cut(s) 265, 603, 1038, 1077, 1965, 2702, 2703
BmrI ACTGGG 1 cut(s) 2444
BmsI GCATC 2 cut(s) 1106, 1344
BmuI ACTGGG 1 cut(s) 2444
BpiI GAAGAC 2 cut(s) 1535, 2091
BplI GAGNNNNNCTC 2 cut(s) 2531, 2563
BpmI CTGGAG 2 cut(s) 717, 2036
Bpu10I CCTNAGC 2 cut(s) 590, 2562
Bpu1102I GCTNAGC 1 cut(s) 1439
BpuMI CCSGG 2 cut(s) 2702, 2703
Bsa29I ATCGAT 1 cut(s) 884
BsaHI GRCGYC 1 cut(s) 416
BsaI GGTCTC 1 cut(s) 1147
BsaXI ACNNNNNCTCC 2 cut(s) 2745, 2775
Bsc4I CCNNNNNNNGG 8 cut(s) 502, 1037, 1082, 1109, 1238, 2251, 2701, 2750
Bse1I ACTGG 5 cut(s) 238, 529, 846, 1989, 2439
BseBI CCWGG 5 cut(s) 265, 603, 1038, 1077, 1965
BseCI ATCGAT 1 cut(s) 884
BseGI GGATG 3 cut(s) 323, 597, 1131
BseLI CCNNNNNNNGG 8 cut(s) 502, 1037, 1082, 1109, 1238, 2251, 2701, 2750
BseMII CTCAG 6 cut(s) 222, 416, 604, 1430, 1761, 2516
BseNI ACTGG 5 cut(s) 238, 529, 846, 1989, 2439
BseRI GAGGAG 1 cut(s) 578
BseSI GKGCMC 1 cut(s) 230
BseXI GCAGC 5 cut(s) 857, 1887, 1890, 2164, 2626
Bsh1236I CGCG 1 cut(s) 2031
BshFI GGCC 9 cut(s) 665, 734, 1036, 1080, 1208, 1310, 1447, 2334, 2585
BshNI GGYRCC 1 cut(s) 415
BshVI ATCGAT 1 cut(s) 884
BsiHKAI GWGCWC 3 cut(s) 294, 982, 1300
BsiHKCI CYCGRG 1 cut(s) 2701
BsiSI CCGG 1 cut(s) 2702
BslFI GGGAC 5 cut(s) 193, 1190, 2459, 2526, 2622
BslI CCNNNNNNNGG 8 cut(s) 502, 1037, 1082, 1109, 1238, 2251, 2701, 2750
BsmAI GTCTC 8 cut(s) 141, 1147, 1187, 1722, 1766, 2207, 2366, 2733
BsmBI CGTCTC 2 cut(s) 1766, 2733
BsmFI GGGAC 5 cut(s) 193, 1190, 2459, 2526, 2622
BsmI GAATGC 4 cut(s) 97, 1341, 2189, 2588
BsnI GGCC 9 cut(s) 665, 734, 1036, 1080, 1208, 1310, 1447, 2334, 2585
Bso31I GGTCTC 1 cut(s) 1147
BsoBI CYCGRG 1 cut(s) 2701
Bsp1286I GDGCHC 4 cut(s) 230, 294, 982, 1300
Bsp1407I TGTACA 2 cut(s) 903, 1839
Bsp143I GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
Bsp1720I GCTNAGC 1 cut(s) 1439
Bsp19I CCATGG 2 cut(s) 1723, 2134
BspACI CCGC 3 cut(s) 456, 2155, 2586
BspANI GGCC 9 cut(s) 665, 734, 1036, 1080, 1208, 1310, 1447, 2334, 2585
BspCNI CTCAG 6 cut(s) 223, 417, 603, 1431, 1762, 2515
BspDI ATCGAT 1 cut(s) 884
BspFNI CGCG 1 cut(s) 2031
BspHI TCATGA 3 cut(s) 1416, 1495, 1798
BspLI GGNNCC 6 cut(s) 262, 274, 417, 1042, 2140, 2610
BspMAI CTGCAG 1 cut(s) 1365
BspMI ACCTGC 1 cut(s) 7
BspPI GGATC 6 cut(s) 267, 280, 586, 880, 889, 1582
BspQI GCTCTTC 1 cut(s) 929
BspT107I GGYRCC 1 cut(s) 415
BspTNI GGTCTC 1 cut(s) 1147
BsrGI TGTACA 2 cut(s) 903, 1839
BsrI ACTGG 5 cut(s) 238, 529, 846, 1989, 2439
BssMI GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
BssNI GRCGYC 1 cut(s) 416
BssT1I CCWWGG 7 cut(s) 255, 411, 1031, 1081, 1209, 1723, 2134
Bst2UI CCWGG 5 cut(s) 265, 603, 1038, 1077, 1965
Bst4CI ACNGT 4 cut(s) 721, 1181, 2301, 2737
Bst6I CTCTTC 1 cut(s) 929
BstACI GRCGYC 1 cut(s) 416
BstAPI GCANNNNNTGC 1 cut(s) 2657
BstAUI TGTACA 2 cut(s) 903, 1839
BstC8I GCNNGC 3 cut(s) 1296, 1445, 2312
BstDSI CCRYGG 4 cut(s) 211, 889, 1723, 2134
BstENI CCTNNNNNAGG 2 cut(s) 500, 1080
BstF5I GGATG 3 cut(s) 323, 597, 1131
BstFNI CGCG 1 cut(s) 2031
BstH2I RGCGCY 2 cut(s) 419, 1439
BstHHI GCGC 3 cut(s) 418, 795, 1438
BstKTI GATC 9 cut(s) 275, 581, 752, 835, 884, 888, 923, 1305, 1590
BstMAI GTCTC 8 cut(s) 141, 1147, 1187, 1722, 1766, 2207, 2366, 2733
BstMBI GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
BstMWI GCNNNNNNNGC 6 cut(s) 234, 851, 1033, 1444, 2648, 2657
BstNI CCWGG 5 cut(s) 265, 603, 1038, 1077, 1965
BstNSI RCATGY 2 cut(s) 904, 1426
BstSCI CCNGG 7 cut(s) 263, 601, 1036, 1075, 1963, 2700, 2701
BstSFI CTRYAG 2 cut(s) 1361, 2297
BstSLI GKGCMC 1 cut(s) 230
BstUI CGCG 1 cut(s) 2031
BstV1I GCAGC 5 cut(s) 857, 1887, 1890, 2164, 2626
BstV2I GAAGAC 2 cut(s) 1535, 2091
BstX2I RGATCY 3 cut(s) 272, 578, 1587
BstYI RGATCY 3 cut(s) 272, 578, 1587
Bsu15I ATCGAT 1 cut(s) 884
BsuRI GGCC 9 cut(s) 665, 734, 1036, 1080, 1208, 1310, 1447, 2334, 2585
BsuTUI ATCGAT 1 cut(s) 884
BtgI CCRYGG 4 cut(s) 211, 889, 1723, 2134
BtsCI GGATG 3 cut(s) 323, 597, 1131
BtsIMutI CAGTG 4 cut(s) 78, 245, 1996, 2643
BveI ACCTGC 1 cut(s) 7
Cac8I GCNNGC 3 cut(s) 1296, 1445, 2312
CaiI CAGNNNCTG 2 cut(s) 1351, 1571
CciI TCATGA 3 cut(s) 1416, 1495, 1798
CfoI GCGC 3 cut(s) 418, 795, 1438
Cfr13I GGNCC 6 cut(s) 261, 786, 1376, 1446, 1503, 2609
Cfr9I CCCGGG 1 cut(s) 2701
ClaI ATCGAT 1 cut(s) 884
CseI GACGC 1 cut(s) 1346
Csp6I GTAC 3 cut(s) 620, 904, 1840
CspCI CAANNNNNGTGG 2 cut(s) 2702, 2737
CviQI GTAC 3 cut(s) 620, 904, 1840
DinI GGCGCC 1 cut(s) 417
DpnI GATC 9 cut(s) 274, 580, 751, 834, 883, 887, 922, 1304, 1589
DpnII GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
DriI GACNNNNNGTC 1 cut(s) 1912
EaeI YGGCCR 2 cut(s) 663, 2583
Eam1104I CTCTTC 1 cut(s) 929
Eam1105I GACNNNNNGTC 1 cut(s) 1912
EarI CTCTTC 1 cut(s) 929
Ecl136II GAGCTC 1 cut(s) 1298
Eco130I CCWWGG 7 cut(s) 255, 411, 1031, 1081, 1209, 1723, 2134
Eco147I AGGCCT 3 cut(s) 1036, 1208, 1310
Eco24I GRGCYC 1 cut(s) 1300
Eco31I GGTCTC 1 cut(s) 1147
Eco32I GATATC 2 cut(s) 156, 1468
Eco47I GGWCC 5 cut(s) 261, 786, 1376, 1503, 2609
Eco47III AGCGCT 1 cut(s) 1437
Eco53kI GAGCTC 1 cut(s) 1298
Eco57I CTGAAG 5 cut(s) 485, 1653, 1869, 2571, 2667
Eco88I CYCGRG 1 cut(s) 2701
EcoICRI GAGCTC 1 cut(s) 1298
EcoNI CCTNNNNNAGG 2 cut(s) 500, 1080
EcoO109I RGGNCCY 3 cut(s) 261, 1376, 2609
EcoRI GAATTC 1 cut(s) 535
EcoRII CCWGG 5 cut(s) 263, 601, 1036, 1075, 1963
EcoRV GATATC 2 cut(s) 156, 1468
EcoT14I CCWWGG 7 cut(s) 255, 411, 1031, 1081, 1209, 1723, 2134
EcoT38I GRGCYC 1 cut(s) 1300
EgeI GGCGCC 1 cut(s) 417
EheI GGCGCC 1 cut(s) 417
ErhI CCWWGG 7 cut(s) 255, 411, 1031, 1081, 1209, 1723, 2134
Esp3I CGTCTC 2 cut(s) 1766, 2733
FaqI GGGAC 5 cut(s) 193, 1190, 2459, 2526, 2622
FbaI TGATCA 1 cut(s) 1302
Fnu4HI GCNGC 6 cut(s) 846, 1876, 1879, 2153, 2586, 2640
FokI GGATG 3 cut(s) 310, 584, 1118
FriOI GRGCYC 1 cut(s) 1300
Fsp4HI GCNGC 6 cut(s) 846, 1876, 1879, 2153, 2586, 2640
GlaI GCGC 3 cut(s) 417, 794, 1437
GluI GCNGC 6 cut(s) 846, 1876, 1879, 2153, 2586, 2640
GsuI CTGGAG 2 cut(s) 717, 2036
HaeII RGCGCY 2 cut(s) 419, 1439
HaeIII GGCC 9 cut(s) 665, 734, 1036, 1080, 1208, 1310, 1447, 2334, 2585
HapII CCGG 1 cut(s) 2702
HgaI GACGC 1 cut(s) 1346
HhaI GCGC 3 cut(s) 418, 795, 1438
Hin1I GRCGYC 1 cut(s) 416
Hin6I GCGC 3 cut(s) 416, 793, 1436
HinP1I GCGC 3 cut(s) 416, 793, 1436
HincII GTYRAC 1 cut(s) 997
HindII GTYRAC 1 cut(s) 997
HindIII AAGCTT 1 cut(s) 512
HpaI GTTAAC 1 cut(s) 997
HpaII CCGG 1 cut(s) 2702
Hpy166II GTNNAC 5 cut(s) 786, 997, 1329, 1842, 2713
Hpy8I GTNNAC 5 cut(s) 786, 997, 1329, 1842, 2713
HpyCH4III ACNGT 4 cut(s) 721, 1181, 2301, 2737
HpyCH4IV ACGT 1 cut(s) 206
HpyF10VI GCNNNNNNNGC 6 cut(s) 234, 851, 1033, 1444, 2648, 2657
HpySE526I ACGT 1 cut(s) 206
Hsp92I GRCGYC 1 cut(s) 416
HspAI GCGC 3 cut(s) 416, 793, 1436
KasI GGCGCC 1 cut(s) 415
Ksp22I TGATCA 1 cut(s) 1302
KspAI GTTAAC 1 cut(s) 997
Kzo9I GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
LguI GCTCTTC 1 cut(s) 929
LmnI GCTCC 3 cut(s) 698, 851, 985
Lsp1109I GCAGC 5 cut(s) 857, 1887, 1890, 2164, 2626
LweI GCATC 2 cut(s) 1106, 1344
MaeII ACGT 1 cut(s) 206
MaeIII GTNAC 6 cut(s) 25, 279, 428, 896, 1276, 1561
MalI GATC 9 cut(s) 274, 580, 751, 834, 883, 887, 922, 1304, 1589
MboI GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
MfeI CAATTG 1 cut(s) 753
MflI RGATCY 3 cut(s) 272, 578, 1587
MhlI GDGCHC 4 cut(s) 230, 294, 982, 1300
MlsI TGGCCA 1 cut(s) 665
MluNI TGGCCA 1 cut(s) 665
Mly113I GGCGCC 1 cut(s) 416
MlyI GAGTC 5 cut(s) 1324, 1330, 2027, 2311, 2368
MmeI TCCRAC 5 cut(s) 509, 1358, 1769, 2605, 2701
Mox20I TGGCCA 1 cut(s) 665
MroXI GAANNNNTTC 1 cut(s) 1830
MscI TGGCCA 1 cut(s) 665
MseI TTAA 4 cut(s) 377, 996, 1736, 2052
MslI CAYNNNNRTG 4 cut(s) 64, 1421, 1494, 1667
Msp20I TGGCCA 1 cut(s) 665
MspA1I CMGCKG 3 cut(s) 317, 456, 2155
MspI CCGG 1 cut(s) 2702
MspR9I CCNGG 7 cut(s) 265, 603, 1038, 1077, 1965, 2702, 2703
MunI CAATTG 1 cut(s) 753
Mva1269I GAATGC 4 cut(s) 97, 1341, 2189, 2588
MvaI CCWGG 5 cut(s) 265, 603, 1038, 1077, 1965
MvnI CGCG 1 cut(s) 2031
MwoI GCNNNNNNNGC 6 cut(s) 234, 851, 1033, 1444, 2648, 2657
NarI GGCGCC 1 cut(s) 416
NciI CCSGG 2 cut(s) 2702, 2703
NcoI CCATGG 2 cut(s) 1723, 2134
NdeII GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
NlaIV GGNNCC 6 cut(s) 262, 274, 417, 1042, 2140, 2610
NmeAIII GCCGAG 1 cut(s) 1037
NmuCI GTSAC 3 cut(s) 25, 279, 896
NspI RCATGY 2 cut(s) 904, 1426
PagI TCATGA 3 cut(s) 1416, 1495, 1798
PceI AGGCCT 3 cut(s) 1036, 1208, 1310
PciI ACATGT 1 cut(s) 900
PciSI GCTCTTC 1 cut(s) 929
PctI GAATGC 4 cut(s) 97, 1341, 2189, 2588
PdmI GAANNNNTTC 1 cut(s) 1830
PflMI CCANNNNNTGG 2 cut(s) 1109, 2750
PkrI GCNGC 6 cut(s) 847, 1877, 1880, 2154, 2587, 2641
PleI GAGTC 5 cut(s) 1324, 1330, 2027, 2311, 2368
PluTI GGCGCC 1 cut(s) 419
PpsI GAGTC 5 cut(s) 1324, 1330, 2027, 2311, 2368
PpuMI RGGWCCY 3 cut(s) 261, 1376, 2609
PscI ACATGT 1 cut(s) 900
Psp124BI GAGCTC 1 cut(s) 1300
Psp5II RGGWCCY 3 cut(s) 261, 1376, 2609
Psp6I CCWGG 5 cut(s) 263, 601, 1036, 1075, 1963
PspGI CCWGG 5 cut(s) 263, 601, 1036, 1075, 1963
PspN4I GGNNCC 6 cut(s) 262, 274, 417, 1042, 2140, 2610
PspPI GGNCC 6 cut(s) 261, 786, 1376, 1446, 1503, 2609
PspPPI RGGWCCY 3 cut(s) 261, 1376, 2609
PstI CTGCAG 1 cut(s) 1365
PstNI CAGNNNCTG 2 cut(s) 1351, 1571
PsuI RGATCY 3 cut(s) 272, 578, 1587
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 3 cut(s) 621, 905, 1841
RsaNI GTAC 3 cut(s) 620, 904, 1840
RseI CAYNNNNRTG 4 cut(s) 64, 1421, 1494, 1667
SacI GAGCTC 1 cut(s) 1300
SapI GCTCTTC 1 cut(s) 929
SaqAI TTAA 4 cut(s) 377, 996, 1736, 2052
SatI GCNGC 6 cut(s) 846, 1876, 1879, 2153, 2586, 2640
Sau3AI GATC 9 cut(s) 272, 578, 749, 832, 881, 885, 920, 1302, 1587
Sau96I GGNCC 6 cut(s) 261, 786, 1376, 1446, 1503, 2609
ScaI AGTACT 1 cut(s) 621
SchI GAGTC 5 cut(s) 1324, 1330, 2027, 2311, 2368
ScrFI CCNGG 7 cut(s) 265, 603, 1038, 1077, 1965, 2702, 2703
SduI GDGCHC 4 cut(s) 230, 294, 982, 1300
SfaNI GCATC 2 cut(s) 1106, 1344
SfcI CTRYAG 2 cut(s) 1361, 2297
SfoI GGCGCC 1 cut(s) 417
SinI GGWCC 5 cut(s) 261, 786, 1376, 1503, 2609
SmaI CCCGGG 1 cut(s) 2703
SmiMI CAYNNNNRTG 4 cut(s) 64, 1421, 1494, 1667
SseBI AGGCCT 3 cut(s) 1036, 1208, 1310
SsiI CCGC 3 cut(s) 456, 2155, 2586
SspDI GGCGCC 1 cut(s) 415
SspI AATATT 4 cut(s) 381, 486, 945, 1935
SstI GAGCTC 1 cut(s) 1300
StuI AGGCCT 3 cut(s) 1036, 1208, 1310
StyD4I CCNGG 7 cut(s) 263, 601, 1036, 1075, 1963, 2700, 2701
StyI CCWWGG 7 cut(s) 255, 411, 1031, 1081, 1209, 1723, 2134
TaaI ACNGT 4 cut(s) 721, 1181, 2301, 2737
TaiI ACGT 1 cut(s) 209
TaqI TCGA 4 cut(s) 884, 1916, 2162, 2740
TatI WGTACW 3 cut(s) 619, 903, 1839
TauI GCSGC 1 cut(s) 2588
Tru1I TTAA 4 cut(s) 377, 996, 1736, 2052
Tru9I TTAA 4 cut(s) 377, 996, 1736, 2052
TscAI CASTG 4 cut(s) 85, 245, 1996, 2650
TseFI GTSAC 3 cut(s) 25, 279, 896
TseI GCWGC 5 cut(s) 845, 1875, 1878, 2152, 2639
Tsp45I GTSAC 3 cut(s) 25, 279, 896
TspGWI ACGGA 2 cut(s) 878, 2715
TspMI CCCGGG 1 cut(s) 2701
TspRI CASTG 4 cut(s) 85, 245, 1996, 2650
Van91I CCANNNNNTGG 2 cut(s) 1109, 2750
VpaK11BI GGWCC 5 cut(s) 261, 786, 1376, 1503, 2609
XagI CCTNNNNNAGG 2 cut(s) 500, 1080
XapI RAATTY 5 cut(s) 334, 359, 535, 1522, 1829
XbaI TCTAGA 1 cut(s) 761
XceI RCATGY 2 cut(s) 904, 1426
XcmI CCANNNNNNNNNTGG 1 cut(s) 673
XmaI CCCGGG 1 cut(s) 2701
XmaJI CCTAGG 2 cut(s) 1031, 1081
XmnI GAANNNNTTC 1 cut(s) 1830
ZrmI AGTACT 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.