Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 711 |
| Acc65I | GGTACC | 1 cut(s) | 302 |
| AccB1I | GGYRCC | 1 cut(s) | 302 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1616 |
| AccBSI | CCGCTC | 1 cut(s) | 1408 |
| AciI | CCGC | 2 cut(s) | 88, 1408 |
| AcoI | YGGCCR | 1 cut(s) | 468 |
| AcsI | RAATTY | 6 cut(s) | 590, 660, 703, 759, 1090, 1482 |
| AcuI | CTGAAG | 3 cut(s) | 275, 456, 591 |
| AfaI | GTAC | 4 cut(s) | 7, 304, 965, 1166 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1616 |
| AflII | CTTAAG | 1 cut(s) | 266 |
| AflIII | ACRYGT | 1 cut(s) | 669 |
| AhlI | ACTAGT | 1 cut(s) | 749 |
| AjnI | CCWGG | 3 cut(s) | 620, 724, 1566 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 464, 496, 843, 875 |
| AluBI | AGCT | 9 cut(s) | 39, 311, 409, 495, 524, 642, 739, 775, 1283 |
| AluI | AGCT | 9 cut(s) | 39, 311, 409, 495, 524, 642, 739, 775, 1283 |
| Alw26I | GTCTC | 2 cut(s) | 1194, 1245 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 112 |
| Ama87I | CYCGRG | 1 cut(s) | 425 |
| AoxI | GGCC | 4 cut(s) | 136, 195, 421, 468 |
| ApaI | GGGCCC | 1 cut(s) | 140 |
| ApeKI | GCWGC | 1 cut(s) | 1451 |
| ApoI | RAATTY | 6 cut(s) | 590, 660, 703, 759, 1090, 1482 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1467, 1499 |
| Asp718I | GGTACC | 1 cut(s) | 302 |
| AspS9I | GGNCC | 4 cut(s) | 136, 137, 1005, 1475 |
| AsuHPI | GGTGA | 1 cut(s) | 1349 |
| AsuII | TTCGAA | 1 cut(s) | 460 |
| AvaI | CYCGRG | 1 cut(s) | 425 |
| AvaII | GGWCC | 2 cut(s) | 1005, 1475 |
| BaeGI | GKGCMC | 1 cut(s) | 140 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 236, 269 |
| BalI | TGGCCA | 1 cut(s) | 470 |
| BanI | GGYRCC | 1 cut(s) | 302 |
| BanII | GRGCYC | 1 cut(s) | 140 |
| BauI | CACGAG | 1 cut(s) | 259 |
| BbvI | GCAGC | 1 cut(s) | 1438 |
| BccI | CCATC | 2 cut(s) | 109, 1035 |
| BceAI | ACGGC | 2 cut(s) | 436, 1114 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 556, 590 |
| BciT130I | CCWGG | 3 cut(s) | 622, 726, 1568 |
| BciVI | GTATCC | 1 cut(s) | 740 |
| BclI | TGATCA | 2 cut(s) | 946, 1227 |
| BcoDI | GTCTC | 2 cut(s) | 1194, 1245 |
| BcuI | ACTAGT | 1 cut(s) | 749 |
| BfaI | CTAG | 3 cut(s) | 525, 750, 1479 |
| BfmI | CTRYAG | 4 cut(s) | 104, 122, 237, 1347 |
| BfrI | CTTAAG | 1 cut(s) | 266 |
| BfuI | GTATCC | 1 cut(s) | 740 |
| BglII | AGATCT | 1 cut(s) | 69 |
| BisI | GCNGC | 1 cut(s) | 1452 |
| BlsI | GCNGC | 1 cut(s) | 1453 |
| Bme1390I | CCNGG | 3 cut(s) | 622, 726, 1568 |
| Bme18I | GGWCC | 2 cut(s) | 1005, 1475 |
| BmeT110I | CYCGRG | 1 cut(s) | 425 |
| BmgT120I | GGNCC | 4 cut(s) | 136, 137, 1005, 1475 |
| BmiI | GGNNCC | 5 cut(s) | 138, 304, 330, 510, 1476 |
| BmrFI | CCNGG | 3 cut(s) | 622, 726, 1568 |
| BmrI | ACTGGG | 1 cut(s) | 14 |
| BmuI | ACTGGG | 1 cut(s) | 14 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1397, 1429 |
| BpmI | CTGGAG | 1 cut(s) | 824 |
| Bpu14I | TTCGAA | 1 cut(s) | 460 |
| BpuEI | CTTGAG | 1 cut(s) | 797 |
| BsaAI | YACGTR | 1 cut(s) | 791 |
| BsaJI | CCNNGG | 5 cut(s) | 157, 424, 484, 1566, 1567 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1611, 1641 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1616 |
| Bse1I | ACTGG | 3 cut(s) | 16, 20, 807 |
| BseBI | CCWGG | 3 cut(s) | 622, 726, 1568 |
| BseDI | CCNNGG | 5 cut(s) | 157, 424, 484, 1566, 1567 |
| BseGI | GGATG | 1 cut(s) | 1338 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1616 |
| BseMII | CTCAG | 5 cut(s) | 263, 326, 522, 854, 1428 |
| BseNI | ACTGG | 3 cut(s) | 16, 20, 807 |
| BseRI | GAGGAG | 1 cut(s) | 1575 |
| BseSI | GKGCMC | 1 cut(s) | 140 |
| BseXI | GCAGC | 1 cut(s) | 1438 |
| BseYI | CCCAGC | 1 cut(s) | 1300 |
| BshFI | GGCC | 4 cut(s) | 138, 197, 423, 470 |
| BshNI | GGYRCC | 1 cut(s) | 302 |
| BsiHKCI | CYCGRG | 1 cut(s) | 425 |
| BslFI | GGGAC | 3 cut(s) | 442, 1392, 1488 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1616 |
| BsmAI | GTCTC | 2 cut(s) | 1194, 1245 |
| BsmFI | GGGAC | 3 cut(s) | 442, 1392, 1488 |
| BsmI | GAATGC | 1 cut(s) | 1454 |
| BsnI | GGCC | 4 cut(s) | 138, 197, 423, 470 |
| BsoBI | CYCGRG | 1 cut(s) | 425 |
| Bsp119I | TTCGAA | 1 cut(s) | 460 |
| Bsp120I | GGGCCC | 1 cut(s) | 136 |
| Bsp1286I | GDGCHC | 1 cut(s) | 140 |
| Bsp143I | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| Bsp19I | CCATGG | 2 cut(s) | 157, 484 |
| BspACI | CCGC | 2 cut(s) | 88, 1408 |
| BspANI | GGCC | 4 cut(s) | 138, 197, 423, 470 |
| BspCNI | CTCAG | 5 cut(s) | 262, 325, 523, 853, 1427 |
| BspHI | TCATGA | 1 cut(s) | 898 |
| BspLI | GGNNCC | 5 cut(s) | 138, 304, 330, 510, 1476 |
| BspMAI | CTGCAG | 1 cut(s) | 126 |
| BspT104I | TTCGAA | 1 cut(s) | 460 |
| BspT107I | GGYRCC | 1 cut(s) | 302 |
| BspTI | CTTAAG | 1 cut(s) | 266 |
| BsrBI | CCGCTC | 1 cut(s) | 1408 |
| BsrI | ACTGG | 3 cut(s) | 16, 20, 807 |
| BssECI | CCNNGG | 5 cut(s) | 157, 424, 484, 1566, 1567 |
| BssMI | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| BssSI | CACGAG | 1 cut(s) | 259 |
| BssT1I | CCWWGG | 2 cut(s) | 157, 484 |
| Bst2BI | CACGAG | 1 cut(s) | 259 |
| Bst2UI | CCWGG | 3 cut(s) | 622, 726, 1568 |
| Bst4CI | ACNGT | 7 cut(s) | 908, 1009, 1120, 1138, 1348, 1603, 1644 |
| Bst6I | CTCTTC | 1 cut(s) | 1593 |
| BstAFI | CTTAAG | 1 cut(s) | 266 |
| BstBAI | YACGTR | 1 cut(s) | 791 |
| BstBI | TTCGAA | 1 cut(s) | 460 |
| BstDEI | CTNAG | 8 cut(s) | 249, 312, 333, 531, 840, 1244, 1414, 1437 |
| BstDSI | CCRYGG | 2 cut(s) | 157, 484 |
| BstF5I | GGATG | 1 cut(s) | 1338 |
| BstKTI | GATC | 7 cut(s) | 63, 72, 576, 717, 949, 1206, 1230 |
| BstMAI | GTCTC | 2 cut(s) | 1194, 1245 |
| BstMBI | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 476 |
| BstNI | CCWGG | 3 cut(s) | 622, 726, 1568 |
| BstNSI | RCATGY | 2 cut(s) | 541, 673 |
| BstSCI | CCNGG | 3 cut(s) | 620, 724, 1566 |
| BstSFI | CTRYAG | 4 cut(s) | 104, 122, 237, 1347 |
| BstSLI | GKGCMC | 1 cut(s) | 140 |
| BstV1I | GCAGC | 1 cut(s) | 1438 |
| BstX2I | RGATCY | 1 cut(s) | 69 |
| BstYI | RGATCY | 1 cut(s) | 69 |
| BsuI | GTATCC | 1 cut(s) | 740 |
| BsuRI | GGCC | 4 cut(s) | 138, 197, 423, 470 |
| BtgI | CCRYGG | 2 cut(s) | 157, 484 |
| BtsCI | GGATG | 1 cut(s) | 1338 |
| BtsI | GCAGTG | 1 cut(s) | 840 |
| BtsIMutI | CAGTG | 6 cut(s) | 9, 299, 840, 1142, 1509, 1640 |
| CaiI | CAGNNNCTG | 1 cut(s) | 112 |
| CciI | TCATGA | 1 cut(s) | 898 |
| Cfr13I | GGNCC | 4 cut(s) | 136, 137, 1005, 1475 |
| Csp6I | GTAC | 4 cut(s) | 6, 303, 964, 1165 |
| CviAII | CATG | 9 cut(s) | 158, 199, 224, 485, 538, 670, 899, 1361, 1616 |
| CviQI | GTAC | 4 cut(s) | 6, 303, 964, 1165 |
| DdeI | CTNAG | 8 cut(s) | 249, 312, 333, 531, 840, 1244, 1414, 1437 |
| DpnI | GATC | 7 cut(s) | 62, 71, 575, 716, 948, 1205, 1229 |
| DpnII | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| DraI | TTTAAA | 1 cut(s) | 888 |
| EaeI | YGGCCR | 1 cut(s) | 468 |
| Eam1104I | CTCTTC | 1 cut(s) | 1593 |
| EarI | CTCTTC | 1 cut(s) | 1593 |
| EciI | GGCGGA | 1 cut(s) | 103 |
| Eco130I | CCWWGG | 2 cut(s) | 157, 484 |
| Eco24I | GRGCYC | 1 cut(s) | 140 |
| Eco32I | GATATC | 1 cut(s) | 684 |
| Eco47I | GGWCC | 2 cut(s) | 1005, 1475 |
| Eco57I | CTGAAG | 3 cut(s) | 275, 456, 591 |
| Eco88I | CYCGRG | 1 cut(s) | 425 |
| EcoO109I | RGGNCCY | 3 cut(s) | 136, 137, 1475 |
| EcoRII | CCWGG | 3 cut(s) | 620, 724, 1566 |
| EcoRV | GATATC | 1 cut(s) | 684 |
| EcoT14I | CCWWGG | 2 cut(s) | 157, 484 |
| EcoT38I | GRGCYC | 1 cut(s) | 140 |
| ErhI | CCWWGG | 2 cut(s) | 157, 484 |
| FaeI | CATG | 9 cut(s) | 161, 202, 227, 488, 541, 673, 902, 1364, 1619 |
| FaqI | GGGAC | 3 cut(s) | 442, 1392, 1488 |
| FatI | CATG | 9 cut(s) | 157, 198, 223, 484, 537, 669, 898, 1360, 1615 |
| FbaI | TGATCA | 2 cut(s) | 946, 1227 |
| Fnu4HI | GCNGC | 1 cut(s) | 1452 |
| FokI | GGATG | 1 cut(s) | 1325 |
| FriOI | GRGCYC | 1 cut(s) | 140 |
| Fsp4HI | GCNGC | 1 cut(s) | 1452 |
| FspBI | CTAG | 3 cut(s) | 525, 750, 1479 |
| GluI | GCNGC | 1 cut(s) | 1452 |
| GsaI | CCCAGC | 1 cut(s) | 1304 |
| GsuI | CTGGAG | 1 cut(s) | 824 |
| HaeIII | GGCC | 4 cut(s) | 138, 197, 423, 470 |
| Hin1II | CATG | 9 cut(s) | 161, 202, 227, 488, 541, 673, 902, 1364, 1619 |
| HincII | GTYRAC | 2 cut(s) | 675, 1268 |
| HindII | GTYRAC | 2 cut(s) | 675, 1268 |
| HindIII | AAGCTT | 3 cut(s) | 37, 737, 773 |
| HinfI | GANTC | 7 cut(s) | 15, 91, 129, 838, 1084, 1238, 1537 |
| HphI | GGTGA | 1 cut(s) | 1349 |
| Hpy166II | GTNNAC | 4 cut(s) | 603, 675, 1268, 1579 |
| Hpy188I | TCNGA | 6 cut(s) | 60, 436, 610, 734, 1096, 1417 |
| Hpy8I | GTNNAC | 4 cut(s) | 603, 675, 1268, 1579 |
| HpyAV | CCTTC | 7 cut(s) | 65, 150, 431, 490, 775, 1412, 1564 |
| HpyCH4III | ACNGT | 7 cut(s) | 908, 1009, 1120, 1138, 1348, 1603, 1644 |
| HpyCH4IV | ACGT | 2 cut(s) | 790, 1575 |
| HpyCH4V | TGCA | 9 cut(s) | 124, 342, 379, 553, 954, 1066, 1216, 1454, 1543 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 476 |
| HpyF3I | CTNAG | 8 cut(s) | 249, 312, 333, 531, 840, 1244, 1414, 1437 |
| HpySE526I | ACGT | 2 cut(s) | 790, 1575 |
| Hsp92II | CATG | 9 cut(s) | 161, 202, 227, 488, 541, 673, 902, 1364, 1619 |
| KpnI | GGTACC | 1 cut(s) | 306 |
| Ksp22I | TGATCA | 2 cut(s) | 946, 1227 |
| Kzo9I | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| Lsp1109I | GCAGC | 1 cut(s) | 1438 |
| MaeI | CTAG | 3 cut(s) | 525, 750, 1479 |
| MaeII | ACGT | 2 cut(s) | 790, 1575 |
| MaeIII | GTNAC | 5 cut(s) | 791, 835, 925, 1138, 1571 |
| MalI | GATC | 7 cut(s) | 62, 71, 575, 716, 948, 1205, 1229 |
| MbiI | CCGCTC | 1 cut(s) | 1408 |
| MboI | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| MboII | GAAGA | 7 cut(s) | 55, 281, 415, 667, 1007, 1198, 1580 |
| MflI | RGATCY | 1 cut(s) | 69 |
| MhlI | GDGCHC | 1 cut(s) | 140 |
| MlsI | TGGCCA | 1 cut(s) | 470 |
| MluNI | TGGCCA | 1 cut(s) | 470 |
| MlyI | GAGTC | 3 cut(s) | 85, 832, 1247 |
| MnlI | CCTC | 8 cut(s) | 187, 258, 434, 556, 1036, 1266, 1377, 1596 |
| Mox20I | TGGCCA | 1 cut(s) | 470 |
| MscI | TGGCCA | 1 cut(s) | 470 |
| MseI | TTAA | 7 cut(s) | 267, 497, 594, 887, 1161, 1211, 1310 |
| MslI | CAYNNNNRTG | 1 cut(s) | 900 |
| Msp20I | TGGCCA | 1 cut(s) | 470 |
| MspCI | CTTAAG | 1 cut(s) | 266 |
| MspR9I | CCNGG | 3 cut(s) | 622, 726, 1568 |
| Mva1269I | GAATGC | 1 cut(s) | 1454 |
| MvaI | CCWGG | 3 cut(s) | 622, 726, 1568 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 476 |
| NcoI | CCATGG | 2 cut(s) | 157, 484 |
| NdeII | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| NlaIII | CATG | 9 cut(s) | 161, 202, 227, 488, 541, 673, 902, 1364, 1619 |
| NlaIV | GGNNCC | 5 cut(s) | 138, 304, 330, 510, 1476 |
| NmuCI | GTSAC | 3 cut(s) | 791, 835, 925 |
| NspI | RCATGY | 2 cut(s) | 541, 673 |
| NspV | TTCGAA | 1 cut(s) | 460 |
| PagI | TCATGA | 1 cut(s) | 898 |
| PasI | CCCWGGG | 1 cut(s) | 1567 |
| PciI | ACATGT | 1 cut(s) | 669 |
| PctI | GAATGC | 1 cut(s) | 1454 |
| PfeI | GAWTC | 4 cut(s) | 15, 129, 1084, 1537 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1616 |
| PfoI | TCCNGGA | 1 cut(s) | 620 |
| PkrI | GCNGC | 1 cut(s) | 1453 |
| PleI | GAGTC | 3 cut(s) | 85, 832, 1246 |
| PpsI | GAGTC | 3 cut(s) | 85, 832, 1246 |
| Ppu21I | YACGTR | 1 cut(s) | 791 |
| PpuMI | RGGWCCY | 1 cut(s) | 1475 |
| PscI | ACATGT | 1 cut(s) | 669 |
| PsiI | TTATAA | 1 cut(s) | 711 |
| Psp5II | RGGWCCY | 1 cut(s) | 1475 |
| Psp6I | CCWGG | 3 cut(s) | 620, 724, 1566 |
| PspFI | CCCAGC | 1 cut(s) | 1300 |
| PspGI | CCWGG | 3 cut(s) | 620, 724, 1566 |
| PspN4I | GGNNCC | 5 cut(s) | 138, 304, 330, 510, 1476 |
| PspOMI | GGGCCC | 1 cut(s) | 136 |
| PspPI | GGNCC | 4 cut(s) | 136, 137, 1005, 1475 |
| PspPPI | RGGWCCY | 1 cut(s) | 1475 |
| PstI | CTGCAG | 1 cut(s) | 126 |
| PstNI | CAGNNNCTG | 1 cut(s) | 112 |
| PsuI | RGATCY | 1 cut(s) | 69 |
| RsaI | GTAC | 4 cut(s) | 7, 304, 965, 1166 |
| RsaNI | GTAC | 4 cut(s) | 6, 303, 964, 1165 |
| RseI | CAYNNNNRTG | 1 cut(s) | 900 |
| SaqAI | TTAA | 7 cut(s) | 267, 497, 594, 887, 1161, 1211, 1310 |
| SatI | GCNGC | 1 cut(s) | 1452 |
| Sau3AI | GATC | 7 cut(s) | 60, 69, 573, 714, 946, 1203, 1227 |
| Sau96I | GGNCC | 4 cut(s) | 136, 137, 1005, 1475 |
| SchI | GAGTC | 3 cut(s) | 85, 832, 1247 |
| ScrFI | CCNGG | 3 cut(s) | 622, 726, 1568 |
| SduI | GDGCHC | 1 cut(s) | 140 |
| SfcI | CTRYAG | 4 cut(s) | 104, 122, 237, 1347 |
| SfuI | TTCGAA | 1 cut(s) | 460 |
| SinI | GGWCC | 2 cut(s) | 1005, 1475 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 900 |
| SmlI | CTYRAG | 2 cut(s) | 266, 776 |
| SmoI | CTYRAG | 2 cut(s) | 266, 776 |
| SpeI | ACTAGT | 1 cut(s) | 749 |
| SsiI | CCGC | 2 cut(s) | 88, 1408 |
| SspI | AATATT | 1 cut(s) | 1327 |
| SspMI | CTAG | 3 cut(s) | 525, 750, 1479 |
| StyD4I | CCNGG | 3 cut(s) | 620, 724, 1566 |
| StyI | CCWWGG | 2 cut(s) | 157, 484 |
| TaaI | ACNGT | 7 cut(s) | 908, 1009, 1120, 1138, 1348, 1603, 1644 |
| TaiI | ACGT | 2 cut(s) | 793, 1578 |
| TaqI | TCGA | 3 cut(s) | 460, 576, 1202 |
| TatI | WGTACW | 1 cut(s) | 963 |
| TfiI | GAWTC | 4 cut(s) | 15, 129, 1084, 1537 |
| Tru1I | TTAA | 7 cut(s) | 267, 497, 594, 887, 1161, 1211, 1310 |
| Tru9I | TTAA | 7 cut(s) | 267, 497, 594, 887, 1161, 1211, 1310 |
| TscAI | CASTG | 6 cut(s) | 16, 299, 840, 1149, 1516, 1647 |
| TseFI | GTSAC | 3 cut(s) | 791, 835, 925 |
| TseI | GCWGC | 1 cut(s) | 1451 |
| Tsp45I | GTSAC | 3 cut(s) | 791, 835, 925 |
| TspDTI | ATGAA | 7 cut(s) | 16, 398, 771, 864, 1235, 1447, 1580 |
| TspGWI | ACGGA | 1 cut(s) | 692 |
| TspRI | CASTG | 6 cut(s) | 16, 299, 840, 1149, 1516, 1647 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1616 |
| Vha464I | CTTAAG | 1 cut(s) | 266 |
| VpaK11BI | GGWCC | 2 cut(s) | 1005, 1475 |
| XapI | RAATTY | 6 cut(s) | 590, 660, 703, 759, 1090, 1482 |
| XceI | RCATGY | 2 cut(s) | 541, 673 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 482 |
| XspI | CTAG | 3 cut(s) | 525, 750, 1479 |