Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1148 |
| AccIII | TCCGGA | 1 cut(s) | 776 |
| AciI | CCGC | 3 cut(s) | 97, 902, 905 |
| AclWI | GGATC | 2 cut(s) | 41, 54 |
| AcoI | YGGCCR | 3 cut(s) | 117, 333, 1139 |
| AcsI | RAATTY | 1 cut(s) | 256 |
| AcuI | CTGAAG | 1 cut(s) | 1065 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 120, 908, 1099, 1148 |
| AflIII | ACRYGT | 1 cut(s) | 771 |
| AgsI | TTSAA | 4 cut(s) | 294, 398, 461, 931 |
| AjnI | CCWGG | 2 cut(s) | 119, 907 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 482, 514, 651, 683 |
| AluBI | AGCT | 5 cut(s) | 233, 305, 331, 360, 453 |
| AluI | AGCT | 5 cut(s) | 233, 305, 331, 360, 453 |
| Alw26I | GTCTC | 1 cut(s) | 891 |
| AlwI | GGATC | 2 cut(s) | 41, 54 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 194, 305 |
| Ama87I | CYCGRG | 1 cut(s) | 1099 |
| Aor13HI | TCCGGA | 1 cut(s) | 776 |
| AoxI | GGCC | 4 cut(s) | 117, 122, 333, 1139 |
| ApeKI | GCWGC | 2 cut(s) | 447, 450 |
| ApoI | RAATTY | 1 cut(s) | 256 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 234, 266 |
| AseI | ATTAAT | 2 cut(s) | 879, 989 |
| AspS9I | GGNCC | 3 cut(s) | 123, 250, 1007 |
| AsuC2I | CCSGG | 2 cut(s) | 1100, 1101 |
| AsuHPI | GGTGA | 2 cut(s) | 193, 1081 |
| AvaI | CYCGRG | 1 cut(s) | 1099 |
| AvaII | GGWCC | 2 cut(s) | 250, 1007 |
| BalI | TGGCCA | 2 cut(s) | 119, 335 |
| BamHI | GGATCC | 1 cut(s) | 46 |
| BanII | GRGCYC | 1 cut(s) | 11 |
| BbsI | GAAGAC | 1 cut(s) | 666 |
| BbvI | GCAGC | 2 cut(s) | 459, 462 |
| BccI | CCATC | 3 cut(s) | 221, 275, 704 |
| BciT130I | CCWGG | 2 cut(s) | 121, 909 |
| BclI | TGATCA | 2 cut(s) | 183, 873 |
| BcnI | CCSGG | 2 cut(s) | 1100, 1101 |
| BcoDI | GTCTC | 1 cut(s) | 891 |
| BfaI | CTAG | 1 cut(s) | 692 |
| BfmI | CTRYAG | 1 cut(s) | 39 |
| BglII | AGATCT | 1 cut(s) | 35 |
| BisI | GCNGC | 3 cut(s) | 448, 451, 905 |
| BlsI | GCNGC | 3 cut(s) | 449, 452, 906 |
| Bme1390I | CCNGG | 4 cut(s) | 121, 909, 1100, 1101 |
| Bme18I | GGWCC | 2 cut(s) | 250, 1007 |
| BmeT110I | CYCGRG | 1 cut(s) | 1099 |
| BmgT120I | GGNCC | 3 cut(s) | 123, 250, 1007 |
| BmiI | GGNNCC | 4 cut(s) | 10, 48, 534, 1008 |
| BmrFI | CCNGG | 4 cut(s) | 121, 909, 1100, 1101 |
| BmsI | GCATC | 1 cut(s) | 931 |
| BpiI | GAAGAC | 1 cut(s) | 666 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 929, 961 |
| BpuEI | CTTGAG | 1 cut(s) | 608 |
| BpuMI | CCSGG | 2 cut(s) | 1100, 1101 |
| BsaBI | GATNNNNATC | 1 cut(s) | 921 |
| BsaJI | CCNNGG | 5 cut(s) | 73, 114, 349, 1098, 1099 |
| BsaWI | WCCGGW | 2 cut(s) | 650, 776 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1143, 1173 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 120, 908, 1099, 1148 |
| Bse3DI | GCAATG | 2 cut(s) | 571, 651 |
| Bse8I | GATNNNNATC | 1 cut(s) | 921 |
| BseAI | TCCGGA | 1 cut(s) | 776 |
| BseBI | CCWGG | 2 cut(s) | 121, 909 |
| BseDI | CCNNGG | 5 cut(s) | 73, 114, 349, 1098, 1099 |
| BseGI | GGATG | 2 cut(s) | 69, 922 |
| BseJI | GATNNNNATC | 1 cut(s) | 921 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 120, 908, 1099, 1148 |
| BseMI | GCAATG | 2 cut(s) | 571, 651 |
| BseMII | CTCAG | 4 cut(s) | 182, 186, 188, 404 |
| BseRI | GAGGAG | 1 cut(s) | 669 |
| BseXI | GCAGC | 2 cut(s) | 459, 462 |
| BshFI | GGCC | 4 cut(s) | 119, 124, 335, 1141 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1099 |
| BsiSI | CCGG | 4 cut(s) | 578, 651, 777, 1100 |
| BslFI | GGGAC | 1 cut(s) | 1020 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 120, 908, 1099, 1148 |
| BsmAI | GTCTC | 1 cut(s) | 891 |
| BsmFI | GGGAC | 1 cut(s) | 1020 |
| BsnI | GGCC | 4 cut(s) | 119, 124, 335, 1141 |
| BsoBI | CYCGRG | 1 cut(s) | 1099 |
| Bsp1286I | GDGCHC | 1 cut(s) | 11 |
| Bsp13I | TCCGGA | 1 cut(s) | 776 |
| Bsp143I | GATC | 4 cut(s) | 35, 46, 183, 873 |
| Bsp19I | CCATGG | 3 cut(s) | 73, 114, 349 |
| BspACI | CCGC | 3 cut(s) | 97, 902, 905 |
| BspANI | GGCC | 4 cut(s) | 119, 124, 335, 1141 |
| BspCNI | CTCAG | 4 cut(s) | 181, 187, 187, 405 |
| BspEI | TCCGGA | 1 cut(s) | 776 |
| BspLI | GGNNCC | 4 cut(s) | 10, 48, 534, 1008 |
| BspMAI | CTGCAG | 1 cut(s) | 43 |
| BspPI | GGATC | 2 cut(s) | 41, 54 |
| BsrDI | GCAATG | 2 cut(s) | 571, 651 |
| BssECI | CCNNGG | 5 cut(s) | 73, 114, 349, 1098, 1099 |
| BssMI | GATC | 4 cut(s) | 35, 46, 183, 873 |
| BssT1I | CCWWGG | 3 cut(s) | 73, 114, 349 |
| Bst2UI | CCWGG | 2 cut(s) | 121, 909 |
| Bst4CI | ACNGT | 4 cut(s) | 164, 814, 894, 1135 |
| Bst6I | CTCTTC | 2 cut(s) | 630, 690 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1055 |
| BstC8I | GCNNGC | 1 cut(s) | 333 |
| BstDEI | CTNAG | 4 cut(s) | 168, 174, 195, 413 |
| BstDSI | CCRYGG | 3 cut(s) | 73, 114, 349 |
| BstF5I | GGATG | 2 cut(s) | 69, 922 |
| BstKTI | GATC | 4 cut(s) | 38, 49, 186, 876 |
| BstMAI | GTCTC | 1 cut(s) | 891 |
| BstMBI | GATC | 4 cut(s) | 35, 46, 183, 873 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 341, 1055 |
| BstNI | CCWGG | 2 cut(s) | 121, 909 |
| BstNSI | RCATGY | 1 cut(s) | 775 |
| BstSCI | CCNGG | 4 cut(s) | 119, 907, 1098, 1099 |
| BstSFI | CTRYAG | 1 cut(s) | 39 |
| BstV1I | GCAGC | 2 cut(s) | 459, 462 |
| BstV2I | GAAGAC | 1 cut(s) | 666 |
| BstX2I | RGATCY | 2 cut(s) | 35, 46 |
| BstYI | RGATCY | 2 cut(s) | 35, 46 |
| BsuRI | GGCC | 4 cut(s) | 119, 124, 335, 1141 |
| BtgI | CCRYGG | 3 cut(s) | 73, 114, 349 |
| BtsCI | GGATG | 2 cut(s) | 69, 922 |
| Cac8I | GCNNGC | 1 cut(s) | 333 |
| CaiI | CAGNNNCTG | 2 cut(s) | 194, 305 |
| Cfr13I | GGNCC | 3 cut(s) | 123, 250, 1007 |
| Cfr9I | CCCGGG | 1 cut(s) | 1099 |
| CviAII | CATG | 9 cut(s) | 51, 74, 100, 115, 350, 707, 772, 1027, 1148 |
| DdeI | CTNAG | 4 cut(s) | 168, 174, 195, 413 |
| DpnI | GATC | 4 cut(s) | 37, 48, 185, 875 |
| DpnII | GATC | 4 cut(s) | 35, 46, 183, 873 |
| DraI | TTTAAA | 1 cut(s) | 556 |
| EaeI | YGGCCR | 3 cut(s) | 117, 333, 1139 |
| Eam1104I | CTCTTC | 2 cut(s) | 630, 690 |
| EarI | CTCTTC | 2 cut(s) | 630, 690 |
| EciI | GGCGGA | 1 cut(s) | 891 |
| Eco130I | CCWWGG | 3 cut(s) | 73, 114, 349 |
| Eco24I | GRGCYC | 1 cut(s) | 11 |
| Eco47I | GGWCC | 2 cut(s) | 250, 1007 |
| Eco57I | CTGAAG | 1 cut(s) | 1065 |
| Eco88I | CYCGRG | 1 cut(s) | 1099 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1007 |
| EcoRII | CCWGG | 2 cut(s) | 119, 907 |
| EcoT14I | CCWWGG | 3 cut(s) | 73, 114, 349 |
| EcoT38I | GRGCYC | 1 cut(s) | 11 |
| ErhI | CCWWGG | 3 cut(s) | 73, 114, 349 |
| FaeI | CATG | 9 cut(s) | 54, 77, 103, 118, 353, 710, 775, 1030, 1151 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 482, 514 |
| FaqI | GGGAC | 1 cut(s) | 1020 |
| FatI | CATG | 9 cut(s) | 50, 73, 99, 114, 349, 706, 771, 1026, 1147 |
| FauI | CCCGC | 1 cut(s) | 104 |
| FbaI | TGATCA | 2 cut(s) | 183, 873 |
| Fnu4HI | GCNGC | 3 cut(s) | 448, 451, 905 |
| FokI | GGATG | 2 cut(s) | 56, 929 |
| FriOI | GRGCYC | 1 cut(s) | 11 |
| Fsp4HI | GCNGC | 3 cut(s) | 448, 451, 905 |
| FspBI | CTAG | 1 cut(s) | 692 |
| GluI | GCNGC | 3 cut(s) | 448, 451, 905 |
| HaeIII | GGCC | 4 cut(s) | 119, 124, 335, 1141 |
| HapII | CCGG | 4 cut(s) | 578, 651, 777, 1100 |
| Hin1II | CATG | 9 cut(s) | 54, 77, 103, 118, 353, 710, 775, 1030, 1151 |
| HincII | GTYRAC | 1 cut(s) | 486 |
| HindII | GTYRAC | 1 cut(s) | 486 |
| HinfI | GANTC | 3 cut(s) | 605, 884, 942 |
| HpaII | CCGG | 4 cut(s) | 578, 651, 777, 1100 |
| HphI | GGTGA | 2 cut(s) | 193, 1081 |
| Hpy166II | GTNNAC | 2 cut(s) | 486, 1111 |
| Hpy188I | TCNGA | 2 cut(s) | 421, 949 |
| Hpy188III | TCNNGA | 3 cut(s) | 398, 432, 777 |
| Hpy8I | GTNNAC | 2 cut(s) | 486, 1111 |
| HpyAV | CCTTC | 6 cut(s) | 100, 355, 455, 634, 944, 1096 |
| HpyCH4III | ACNGT | 4 cut(s) | 164, 814, 894, 1135 |
| HpyCH4IV | ACGT | 1 cut(s) | 764 |
| HpyCH4V | TGCA | 6 cut(s) | 41, 204, 759, 922, 986, 1123 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 341, 1055 |
| HpyF3I | CTNAG | 4 cut(s) | 168, 174, 195, 413 |
| HpySE526I | ACGT | 1 cut(s) | 764 |
| Hsp92II | CATG | 9 cut(s) | 54, 77, 103, 118, 353, 710, 775, 1030, 1151 |
| Kpn2I | TCCGGA | 1 cut(s) | 776 |
| Ksp22I | TGATCA | 2 cut(s) | 183, 873 |
| Kzo9I | GATC | 4 cut(s) | 35, 46, 183, 873 |
| LmnI | GCTCC | 2 cut(s) | 14, 230 |
| Lsp1109I | GCAGC | 2 cut(s) | 459, 462 |
| LweI | GCATC | 1 cut(s) | 931 |
| MaeI | CTAG | 1 cut(s) | 692 |
| MaeII | ACGT | 1 cut(s) | 764 |
| MaeIII | GTNAC | 1 cut(s) | 424 |
| MalI | GATC | 4 cut(s) | 37, 48, 185, 875 |
| MboI | GATC | 4 cut(s) | 35, 46, 183, 873 |
| MboII | GAAGA | 3 cut(s) | 647, 671, 707 |
| MflI | RGATCY | 2 cut(s) | 35, 46 |
| MhlI | GDGCHC | 1 cut(s) | 11 |
| MlsI | TGGCCA | 2 cut(s) | 119, 335 |
| MluCI | AATT | 2 cut(s) | 256, 320 |
| MluNI | TGGCCA | 2 cut(s) | 119, 335 |
| MlyI | GAGTC | 2 cut(s) | 599, 893 |
| MmeI | TCCRAC | 1 cut(s) | 36 |
| MnlI | CCTC | 7 cut(s) | 66, 241, 647, 691, 720, 841, 1020 |
| Mox20I | TGGCCA | 2 cut(s) | 119, 335 |
| MroI | TCCGGA | 1 cut(s) | 776 |
| MscI | TGGCCA | 2 cut(s) | 119, 335 |
| MseI | TTAA | 5 cut(s) | 555, 624, 879, 989, 1177 |
| Msp20I | TGGCCA | 2 cut(s) | 119, 335 |
| MspI | CCGG | 4 cut(s) | 578, 651, 777, 1100 |
| MspR9I | CCNGG | 4 cut(s) | 121, 909, 1100, 1101 |
| MvaI | CCWGG | 2 cut(s) | 121, 909 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 341, 1055 |
| NciI | CCSGG | 2 cut(s) | 1100, 1101 |
| NcoI | CCATGG | 3 cut(s) | 73, 114, 349 |
| NdeII | GATC | 4 cut(s) | 35, 46, 183, 873 |
| NlaIII | CATG | 9 cut(s) | 54, 77, 103, 118, 353, 710, 775, 1030, 1151 |
| NlaIV | GGNNCC | 4 cut(s) | 10, 48, 534, 1008 |
| NmuCI | GTSAC | 1 cut(s) | 424 |
| NspI | RCATGY | 1 cut(s) | 775 |
| PciI | ACATGT | 1 cut(s) | 771 |
| PfeI | GAWTC | 1 cut(s) | 942 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1148 |
| PkrI | GCNGC | 3 cut(s) | 449, 452, 906 |
| PleI | GAGTC | 2 cut(s) | 599, 892 |
| PpsI | GAGTC | 2 cut(s) | 599, 892 |
| PpuMI | RGGWCCY | 1 cut(s) | 1007 |
| PscI | ACATGT | 1 cut(s) | 771 |
| PshBI | ATTAAT | 2 cut(s) | 879, 989 |
| Psp5II | RGGWCCY | 1 cut(s) | 1007 |
| Psp6I | CCWGG | 2 cut(s) | 119, 907 |
| PspGI | CCWGG | 2 cut(s) | 119, 907 |
| PspN4I | GGNNCC | 4 cut(s) | 10, 48, 534, 1008 |
| PspPI | GGNCC | 3 cut(s) | 123, 250, 1007 |
| PspPPI | RGGWCCY | 1 cut(s) | 1007 |
| PstI | CTGCAG | 1 cut(s) | 43 |
| PstNI | CAGNNNCTG | 2 cut(s) | 194, 305 |
| PsuI | RGATCY | 2 cut(s) | 35, 46 |
| SaqAI | TTAA | 5 cut(s) | 555, 624, 879, 989, 1177 |
| SatI | GCNGC | 3 cut(s) | 448, 451, 905 |
| Sau3AI | GATC | 4 cut(s) | 35, 46, 183, 873 |
| Sau96I | GGNCC | 3 cut(s) | 123, 250, 1007 |
| SchI | GAGTC | 2 cut(s) | 599, 893 |
| ScrFI | CCNGG | 4 cut(s) | 121, 909, 1100, 1101 |
| SduI | GDGCHC | 1 cut(s) | 11 |
| SfaNI | GCATC | 1 cut(s) | 931 |
| SfcI | CTRYAG | 1 cut(s) | 39 |
| SinI | GGWCC | 2 cut(s) | 250, 1007 |
| SmaI | CCCGGG | 1 cut(s) | 1101 |
| SmlI | CTYRAG | 1 cut(s) | 587 |
| SmoI | CTYRAG | 1 cut(s) | 587 |
| Sse9I | AATT | 2 cut(s) | 256, 320 |
| SsiI | CCGC | 3 cut(s) | 97, 902, 905 |
| SspI | AATATT | 1 cut(s) | 516 |
| SspMI | CTAG | 1 cut(s) | 692 |
| StyD4I | CCNGG | 4 cut(s) | 119, 907, 1098, 1099 |
| StyI | CCWWGG | 3 cut(s) | 73, 114, 349 |
| TaaI | ACNGT | 4 cut(s) | 164, 814, 894, 1135 |
| TaiI | ACGT | 1 cut(s) | 767 |
| TaqI | TCGA | 1 cut(s) | 1012 |
| TasI | AATT | 2 cut(s) | 256, 320 |
| TauI | GCSGC | 1 cut(s) | 907 |
| TfiI | GAWTC | 1 cut(s) | 942 |
| Tru1I | TTAA | 5 cut(s) | 555, 624, 879, 989, 1177 |
| Tru9I | TTAA | 5 cut(s) | 555, 624, 879, 989, 1177 |
| TseFI | GTSAC | 1 cut(s) | 424 |
| TseI | GCWGC | 2 cut(s) | 447, 450 |
| Tsp45I | GTSAC | 1 cut(s) | 424 |
| TspDTI | ATGAA | 3 cut(s) | 119, 490, 1043 |
| TspGWI | ACGGA | 1 cut(s) | 1107 |
| TspMI | CCCGGG | 1 cut(s) | 1099 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1148 |
| VpaK11BI | GGWCC | 2 cut(s) | 250, 1007 |
| VspI | ATTAAT | 2 cut(s) | 879, 989 |
| XapI | RAATTY | 1 cut(s) | 256 |
| XceI | RCATGY | 1 cut(s) | 775 |
| XmaI | CCCGGG | 1 cut(s) | 1099 |
| XspI | CTAG | 1 cut(s) | 692 |