MD16G1058100.v1.1

Cytochrome P450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000078
Physical Location & Seq
Reverse (-)
4140068 .. 4142408
2341 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1058100.v1.1.491

Sequence Viewer

Length: 1605 bp
ATGGGCAAAGTTAAAAGCAGTGCGAAAATGCCTTCTTCGGGAGGCCTAGCAGTCATTCTTTCAAGCTTTTTGTGTGTGCTCCTCGTCTTCCTTCTCATCAAGATCCTTCGAAGACTTTGGTGGACTCCGACTCGCATACAGAAGCAGATGGCTCTGCAGGGCATCAGAGGCCCTCCTTACAGACTTATCCACGGAAACACCAAACAAGTCTCTCACATGATGAAGGAAATTATGAACAGACCCAAAAATTTATCGCACGACATATTTTCTCGAGTTCAACCACACATTCACTCTTGGACTCAGATATACGGTAACCCGGAATCTCATGTTAAATGTGGTAAGAATTATCTTCAATGGCATGGTTCTAGAGCTCAGCTGGTTATTACAGAACCTGAGTTATGCAAAGAGATACTTAATAACAAAGAGAGGGCTTATGGGAAAAGAGAGACCCCGAAATTTTTGAAGAAGCTATTAGGAGATGGTCTTGTCACAACAACCGAAGCTGAAAAATGGGCAAAATTGCGGAAGCTGGCTACCCATGCCTTCCATGGAGAAAACTTAAAAAGTATGATTCCGGAAATGGTAGCAAGTGCTGAGACAATGCTCGGAAGGTGGGAAGATTATGTAGGGAAAGAGATTGAGGTGTTTGAAGAGTTTAGGTTGTTCACTTCAGAAGTGATTTCTAGAACAGCTTTTGGCAGCAGTTACATAGAAGGCAAGAACATCTTTGAGATGTTGATCAAGTTGGCCTTCCTAGTGTTTAAAAATTCTCTAACAGTCAGGGTTCCTGGCATCAGTAAACTTTTCAAAACCAACGATGAGGTCGAATCAGACAAACTTGAGAAAGAAGTACATGGCTCCATAACAGAGATAGTCAAGAAACGAGAAAAGAAGGCAATGACAGGACAAAAGGAGAGTTTCGGGAGTGATTTTCTCGGATTACTTTTGAAGGCTCATCACGATGCAAATGAGAACCAGAGGGTTTCGGTGGATGAGTTGGTTGATGAGTGCAAGACGTTTTACTTTGCAGGACAAGAAACCACTAATACTTTGCTTGCCTGGACTGTGTTTCTTCTAGCACTCCATACGGATTGGCAAGAGGAAGCAAGAAAGGAGGTGATGGACGTATTCGGCAAACAAACTCCAAATCCTGATGATTTTTCCAAACTGAAAACAATGAGTATGATTTTCTACGAGTCTCAAAGGTTATATCCCCCTCTTGATTCTTTAGTAAGGAAAGTTGATAGGGAAGTTAGACTGGGGAATGTCATTGTCCCACCTAACGTTGAATTGATTCTCTCAAATCTAGCATTTCAGCATGATCCTCGAATCTGGGGAGAAGACTCGCAACTTTTCAAACCAGAGAGATTCGCAGAAGGGATTGCTAAAGCCACTAACAATACCATAAGCGCTTTCATACCCTTTGGAGTGGGACCTCGAAGCTGTGTTGGGTTGAACTTTGCAACCATTGAAGCAAAGATTGCTCTGTCAATGATTCTACAACGCTACTCCTTCACTCTTTCCCCAGGTTACATCCACTTGCCTGTTCATTATTTGACAATGCGGCCACAACATGGAATTCAGGTAATGCTACACTCACTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00905 map00905
Pfam Domains
Protein Families
p450

Protein Analysis

535

Amino Acids

60.97

Weight (kDa)

9.22

Isoelectric Point (pI)

34.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 109 - 510 2.9e-73 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000078)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g15240 FvH4_3g15270 FvH4_3g15280 FvH4_3g32750 FvH4_3g32760 FvH4_3g32760 FvH4_3g32880 FvH4_4g08190 FvH4_4g29160 FvH4_4g29370 FvH4_4g29400 FvH4_4g29420 FvH4_4g29420 FvH4_4g29420 FvH4_4g29430 FvH4_4g29440 FvH4_4g29450 FvH4_4g29450 FvH4_4g29450 FvH4_4g29460 FvH4_4g29461 FvH4_4g29462 FvH4_4g29463 FvH4_4g29480 FvH4_4g29481 FvH4_4g29482 FvH4_4g29483 FvH4_4g29484 FvH4_6g26720 FvH4_6g26720
malus_domestica MD00G1065200.v1.1 MD06G1145800.v1.1 MD06G1146000.v1.1 MD06G1146700.v1.1 MD13G1031000.v1.1 MD13G1033900.v1.1 MD13G1057000.v1.1 MD13G1057100.v1.1 MD13G1057200.v1.1 MD13G1057400.v1.1 MD13G1057800.v1.1 MD13G1058000.v1.1 MD13G1058100.v1.1 MD13G1058200.v1.1 MD13G1058300.v1.1 MD13G1058500.v1.1 MD13G1058600.v1.1 MD13G1058700.v1.1 MD16G1035700.v1.1 MD16G1056400.v1.1 MD16G1056600.v1.1 MD16G1056700.v1.1 MD16G1056900.v1.1 MD16G1058100.v1.1
prunus_persica Prupe.1G291100_v2.0.a1 Prupe.1G291200_v2.0.a1 Prupe.1G291500_v2.0.a1 Prupe.1G291600_v2.0.a1 Prupe.1G291700_v2.0.a1 Prupe.1G291800_v2.0.a1 Prupe.1G291900_v2.0.a1 Prupe.1G292000_v2.0.a1 Prupe.1G292100_v2.0.a1 Prupe.1G292400_v2.0.a1 Prupe.1G292500_v2.0.a1 Prupe.1G292600_v2.0.a1 Prupe.1G292700_v2.0.a1 Prupe.1G292800_v2.0.a1 Prupe.1G296000_v2.0.a1 Prupe.1G296100_v2.0.a1 Prupe.1G296300_v2.0.a1 Prupe.1G296600_v2.0.a1 Prupe.1G296900_v2.0.a1 Prupe.1G534600_v2.0.a1 Prupe.5G046700_v2.0.a1 Prupe.8G021800_v2.0.a1 Prupe.8G026400_v2.0.a1
pyrus_communis pycom06g13510 pycom06g13530 pycom06g13540 pycom06g13570 pycom06g13590 pycom06g13620 pycom13g02710 pycom13g02930 pycom13g05100 pycom13g05110 pycom13g05140 pycom13g05160 pycom13g05170 pycom13g05190 pycom13g05200 pycom13g05210 pycom13g05220 pycom16g03060 pycom16g04940 pycom16g04960 pycom16g04990 pycom16g05000 pycom16g05080
rosa_chinensis RchiOBHm_Chr1g0337981 RchiOBHm_Chr1g0337991 RchiOBHm_Chr1g0339431 RchiOBHm_Chr4g0402401 RchiOBHm_Chr4g0420571 RchiOBHm_Chr4g0420601 RchiOBHm_Chr4g0420611 RchiOBHm_Chr4g0420621 RchiOBHm_Chr4g0420631 RchiOBHm_Chr4g0437211 RchiOBHm_Chr4g0437221 RchiOBHm_Chr4g0437251 RchiOBHm_Chr4g0437431 RchiOBHm_Chr4g0437441 RchiOBHm_Chr4g0437491 RchiOBHm_Chr4g0437501 RchiOBHm_Chr4g0437511 RchiOBHm_Chr4g0437521 RchiOBHm_Chr4g0437531 RchiOBHm_Chr4g0437541 RchiOBHm_Chr4g0437551 RchiOBHm_Chr4g0437591 RchiOBHm_Chr4g0437631 RchiOBHm_Chr4g0437641 RchiOBHm_Chr4g0437671 RchiOBHm_Chr4g0437681 RchiOBHm_Chr4g0437711 RchiOBHm_Chr4g0437731 RchiOBHm_Chr4g0437741 RchiOBHm_Chr4g0437751 RchiOBHm_Chr5g0025681 RchiOBHm_Chr5g0025711 RchiOBHm_Chr5g0025731 RchiOBHm_Chr5g0059841 RchiOBHm_Chr6g0272651
rosa_laevigata RLG00000006420 RLG00000006421 RLG00000006423 RLG00000006426 RLG00000006427 RLG00000006428 RLG00000006429 RLG00000006431 RLG00000006433 RLG00000006434 RLG00000006435 RLG00000006436 RLG00000006437 RLG00000006455 RLG00000007746 RLG00000007747 RLG00000007748 RLG00000007749 RLG00000007750 RLG00000009091 RLG00000009092 RLG00000009095 RLG00000009096 RLG00000017856 RLG00000028094 RLG00000029230 RLG00000029231 RLG00000030008 RLG00000032859 RLG00000032860 RLG00000032861 RLG00000032865 RLG00000032868 RLG00000035332
rosa_multiflora Rmu_co7986016.1_g000001 Rmu_co8226539.1_g000001 Rmu_co8349613.1_g000001 Rmu_sc0000272.1_g000014 Rmu_sc0000591.1_g000003 Rmu_sc0000613.1_g000013 Rmu_sc0001629.1_g000022 Rmu_sc0001629.1_g000025 Rmu_sc0002131.1_g000027 Rmu_sc0002137.1_g000006 Rmu_sc0002137.1_g000014 Rmu_sc0002137.1_g000019 Rmu_sc0002472.1_g000002 Rmu_sc0002472.1_g000012 Rmu_sc0002472.1_g000018 Rmu_sc0002472.1_g000034 Rmu_sc0002472.1_g000040 Rmu_sc0002472.1_g000051 Rmu_sc0002638.1_g000005 Rmu_sc0002638.1_g000010 Rmu_sc0002638.1_g000025 Rmu_sc0002638.1_g000030 Rmu_sc0002638.1_g000037 Rmu_sc0002638.1_g000042 Rmu_sc0004339.1_g000019 Rmu_sc0005580.1_g000013 Rmu_sc0006617.1_g000002 Rmu_sc0006986.1_g000003 Rmu_sc0006986.1_g000017 Rmu_sc0008352.1_g000001 Rmu_sc0008352.1_g000002 Rmu_sc0009431.1_g000011 Rmu_sc0011080.1_g000003 Rmu_sc0011080.1_g000006 Rmu_sc0016549.1_g000001 Rmu_sc0016549.1_g000002 Rmu_sc0017154.1_g000001 Rmu_sc0017155.1_g000001 Rmu_sc0028438.1_g000001 Rmu_sc0041413.1_g000001 Rmu_ssc0000330.1_g000022
rosa_roxburghii Rroxscaffold_1G00020750 Rroxscaffold_1G00053460 Rroxscaffold_1G00054250 Rroxscaffold_1G00054260 Rroxscaffold_1G00054270 Rroxscaffold_1G00054290 Rroxscaffold_2G00120210 Rroxscaffold_4G00313460 Rroxscaffold_4G00313470 Rroxscaffold_4G00330630 Rroxscaffold_5G00347070 Rroxscaffold_5G00347110 Rroxscaffold_5G00378670 Rroxscaffold_5G00378690 Rroxscaffold_5G00378720 Rroxscaffold_5G00378730 Rroxscaffold_5G00378740 Rroxscaffold_5G00378760 Rroxscaffold_5G00378780 Rroxscaffold_5G00378790 Rroxscaffold_5G00378800 Rroxscaffold_5G00378810 Rroxscaffold_5G00378820 Rroxscaffold_5G00378840 Rroxscaffold_5G00378860 Rroxscaffold_5G00378870 Rroxscaffold_7G00196060
rosa_rugosa Rorug01G0142200.1 Rorug01G0332800 Rorug02G0012200 Rorug02G0248300 Rorug03G0219000 Rorug03G0219100 Rorug03G0219200 Rorug04G0035100 Rorug04G0301500 Rorug04G0301500 Rorug04G0301700 Rorug04G0301800 Rorug04G0301800 Rorug04G0301900 Rorug04G0302000 Rorug04G0302500 Rorug04G0302600 Rorug04G0302700 Rorug04G0302800 Rorug04G0302900 Rorug04G0303000 Rorug04G0303000 Rorug04G0303100 Rorug04G0303200 Rorug04G0303300 Rorug04G0303400 Rorug04G0303500 Rorug04G0303500 Rorug05G0087800 Rorug05G0088200 Rorug05G0326300 Rorug06G0173000
rosa_samantha Rh2AG222400 Rh4DG224800 Rh4DG359900 Rh4DG360000 Rh4DG360100 Rh4DG360200 Rh4DG360300 Rh4DG360500 Rh4DG360700 Rh4DG360800 Rh4DG360900 Rh4DG361000 Rh5DG177900 Rh5DG178200 Rh5DG178400 Rh6CG187800
rosa_wichuraiana Rw1G013580 Rw4G009020 Rw4G019580 Rw4G030990 Rw4G031000 Rw4G031220 Rw4G031230 Rw4G031240 Rw4G031250 Rw4G031260 Rw4G031270 Rw4G031280 Rw4G031290 Rw4G031310 Rw4G031320 Rw4G031330 Rw4G031340 Rw4G031360 Rw4G031370 Rw5G016310 Rw5G016320 Rw5G016330 Rw5G036760 Rw6G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1574
AccIII TCCGGA 1 cut(s) 574
AciI CCGC 2 cut(s) 523, 1564
AclI AACGTT 1 cut(s) 1284
AclWI GGATC 2 cut(s) 97, 1316
AcoI YGGCCR 1 cut(s) 1565
AcsI RAATTY 4 cut(s) 247, 455, 766, 1578
AcuI CTGAAG 1 cut(s) 654
AfaI GTAC 1 cut(s) 852
AfeI AGCGCT 1 cut(s) 1411
AfiI CCNNNNNNNGG 2 cut(s) 38, 1574
AjnI CCWGG 3 cut(s) 787, 1058, 1525
AluBI AGCT 8 cut(s) 66, 371, 376, 469, 503, 529, 692, 1443
AluI AGCT 8 cut(s) 66, 371, 376, 469, 503, 529, 692, 1443
Alw21I GWGCWC 2 cut(s) 81, 373
Alw26I GTCTC 4 cut(s) 214, 440, 590, 1203
AlwI GGATC 2 cut(s) 97, 1316
AlwNI CAGNNNCTG 1 cut(s) 392
Ama87I CYCGRG 1 cut(s) 270
Aor13HI TCCGGA 1 cut(s) 574
Aor51HI AGCGCT 1 cut(s) 1411
AoxI GGCC 4 cut(s) 43, 169, 747, 1565
ApeKI GCWGC 1 cut(s) 699
ApoI RAATTY 4 cut(s) 247, 455, 766, 1578
Asp700I GAANNNNTTC 1 cut(s) 1293
AspLEI GCGC 1 cut(s) 1412
AspS9I GGNCC 2 cut(s) 170, 1433
AsuC2I CCSGG 1 cut(s) 317
AsuHPI GGTGA 1 cut(s) 1129
AsuII TTCGAA 1 cut(s) 109
AvaI CYCGRG 1 cut(s) 270
AvaII GGWCC 1 cut(s) 1433
BanII GRGCYC 1 cut(s) 373
BbsI GAAGAC 3 cut(s) 79, 118, 1347
Bbv12I GWGCWC 2 cut(s) 81, 373
BbvI GCAGC 1 cut(s) 711
BccI CCATC 3 cut(s) 142, 473, 1114
BcgI CGANNNNNNTGC 2 cut(s) 1307, 1341
BciT130I CCWGG 3 cut(s) 789, 1060, 1527
BclI TGATCA 1 cut(s) 738
BcnI CCSGG 1 cut(s) 317
BcoDI GTCTC 4 cut(s) 214, 440, 590, 1203
BfaI CTAG 6 cut(s) 47, 366, 684, 755, 1076, 1307
BfmI CTRYAG 1 cut(s) 155
BfoI RGCGCY 1 cut(s) 1413
BisI GCNGC 2 cut(s) 700, 1565
BlpI GCTNAGC 1 cut(s) 372
BlsI GCNGC 2 cut(s) 701, 1566
Bme1390I CCNGG 4 cut(s) 317, 789, 1060, 1527
Bme18I GGWCC 1 cut(s) 1433
BmeT110I CYCGRG 1 cut(s) 270
BmgT120I GGNCC 2 cut(s) 170, 1433
BmiI GGNNCC 3 cut(s) 786, 859, 1434
BmrFI CCNGG 4 cut(s) 317, 789, 1060, 1527
BmrI ACTGGG 1 cut(s) 1268
BmsI GCATC 3 cut(s) 171, 801, 952
BmuI ACTGGG 1 cut(s) 1268
BpiI GAAGAC 3 cut(s) 79, 118, 1347
Bpu1102I GCTNAGC 1 cut(s) 372
Bpu14I TTCGAA 1 cut(s) 109
BpuEI CTTGAG 1 cut(s) 860
BpuMI CCSGG 1 cut(s) 317
BsaBI GATNNNNATC 1 cut(s) 737
BsaI GGTCTC 1 cut(s) 440
BsaJI CCNNGG 3 cut(s) 190, 547, 1525
BsaWI WCCGGW 1 cut(s) 574
Bsc4I CCNNNNNNNGG 2 cut(s) 38, 1574
Bse1I ACTGG 1 cut(s) 1263
Bse3DI GCAATG 1 cut(s) 903
Bse8I GATNNNNATC 1 cut(s) 737
BseAI TCCGGA 1 cut(s) 574
BseBI CCWGG 3 cut(s) 789, 1060, 1527
BseDI CCNNGG 3 cut(s) 190, 547, 1525
BseGI GGATG 2 cut(s) 997, 1533
BseJI GATNNNNATC 1 cut(s) 737
BseLI CCNNNNNNNGG 2 cut(s) 38, 1574
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 4 cut(s) 314, 384, 386, 585
BseNI ACTGG 1 cut(s) 1263
BseRI GAGGAG 1 cut(s) 71
BseXI GCAGC 1 cut(s) 711
BshFI GGCC 4 cut(s) 45, 171, 749, 1567
BsiHKAI GWGCWC 2 cut(s) 81, 373
BsiHKCI CYCGRG 1 cut(s) 270
BsiSI CCGG 2 cut(s) 317, 575
BslFI GGGAC 2 cut(s) 1259, 1446
BslI CCNNNNNNNGG 2 cut(s) 38, 1574
BsmAI GTCTC 4 cut(s) 214, 440, 590, 1203
BsmFI GGGAC 2 cut(s) 1259, 1446
BsnI GGCC 4 cut(s) 45, 171, 749, 1567
Bso31I GGTCTC 1 cut(s) 440
BsoBI CYCGRG 1 cut(s) 270
Bsp119I TTCGAA 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 81, 373
Bsp13I TCCGGA 1 cut(s) 574
Bsp143I GATC 3 cut(s) 102, 738, 1321
Bsp1720I GCTNAGC 1 cut(s) 372
Bsp19I CCATGG 1 cut(s) 547
BspACI CCGC 2 cut(s) 523, 1564
BspANI GGCC 4 cut(s) 45, 171, 749, 1567
BspCNI CTCAG 4 cut(s) 313, 385, 385, 586
BspEI TCCGGA 1 cut(s) 574
BspLI GGNNCC 3 cut(s) 786, 859, 1434
BspMAI CTGCAG 1 cut(s) 159
BspPI GGATC 2 cut(s) 97, 1316
BspT104I TTCGAA 1 cut(s) 109
BspTNI GGTCTC 1 cut(s) 440
BsrDI GCAATG 1 cut(s) 903
BsrI ACTGG 1 cut(s) 1263
BssECI CCNNGG 3 cut(s) 190, 547, 1525
BssMI GATC 3 cut(s) 102, 738, 1321
BssT1I CCWWGG 1 cut(s) 547
Bst2UI CCWGG 3 cut(s) 789, 1060, 1527
Bst4CI ACNGT 3 cut(s) 311, 778, 1066
Bst6I CTCTTC 1 cut(s) 645
BstAPI GCANNNNNTGC 1 cut(s) 1481
BstBI TTCGAA 1 cut(s) 109
BstC8I GCNNGC 2 cut(s) 531, 1056
BstDEI CTNAG 4 cut(s) 300, 372, 393, 594
BstDSI CCRYGG 2 cut(s) 190, 547
BstEII GGTNACC 1 cut(s) 311
BstF5I GGATG 2 cut(s) 997, 1533
BstH2I RGCGCY 1 cut(s) 1413
BstHHI GCGC 1 cut(s) 1412
BstKTI GATC 3 cut(s) 105, 741, 1324
BstMAI GTCTC 4 cut(s) 214, 440, 590, 1203
BstMBI GATC 3 cut(s) 102, 738, 1321
BstMWI GCNNNNNNNGC 3 cut(s) 168, 539, 1481
BstNI CCWGG 3 cut(s) 789, 1060, 1527
BstPI GGTNACC 1 cut(s) 311
BstSCI CCNGG 4 cut(s) 315, 787, 1058, 1525
BstSFI CTRYAG 1 cut(s) 155
BstV1I GCAGC 1 cut(s) 711
BstV2I GAAGAC 3 cut(s) 79, 118, 1347
BstX2I RGATCY 1 cut(s) 102
BstYI RGATCY 1 cut(s) 102
BsuRI GGCC 4 cut(s) 45, 171, 749, 1567
BtgI CCRYGG 2 cut(s) 190, 547
BtsCI GGATG 2 cut(s) 997, 1533
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 25
Cac8I GCNNGC 2 cut(s) 531, 1056
CaiI CAGNNNCTG 1 cut(s) 392
CfoI GCGC 1 cut(s) 1412
Cfr13I GGNCC 2 cut(s) 170, 1433
Csp6I GTAC 1 cut(s) 851
CviAII CATG 8 cut(s) 217, 326, 359, 539, 548, 854, 1319, 1574
CviQI GTAC 1 cut(s) 851
DdeI CTNAG 4 cut(s) 300, 372, 393, 594
DpnI GATC 3 cut(s) 104, 740, 1323
DpnII GATC 3 cut(s) 102, 738, 1321
DraI TTTAAA 1 cut(s) 763
EaeI YGGCCR 1 cut(s) 1565
Eam1104I CTCTTC 1 cut(s) 645
EarI CTCTTC 1 cut(s) 645
Ecl136II GAGCTC 1 cut(s) 371
Eco130I CCWWGG 1 cut(s) 547
Eco147I AGGCCT 1 cut(s) 45
Eco24I GRGCYC 1 cut(s) 373
Eco31I GGTCTC 1 cut(s) 440
Eco47I GGWCC 1 cut(s) 1433
Eco47III AGCGCT 1 cut(s) 1411
Eco53kI GAGCTC 1 cut(s) 371
Eco57I CTGAAG 1 cut(s) 654
Eco88I CYCGRG 1 cut(s) 270
Eco91I GGTNACC 1 cut(s) 311
EcoICRI GAGCTC 1 cut(s) 371
EcoO109I RGGNCCY 2 cut(s) 170, 1433
EcoO65I GGTNACC 1 cut(s) 311
EcoRI GAATTC 1 cut(s) 1578
EcoRII CCWGG 3 cut(s) 787, 1058, 1525
EcoT14I CCWWGG 1 cut(s) 547
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 547
FaeI CATG 8 cut(s) 220, 329, 362, 542, 551, 857, 1322, 1577
FalI AAGNNNNNCTT 6 cut(s) 396, 428, 710, 742, 734, 766
FaqI GGGAC 2 cut(s) 1259, 1446
FatI CATG 8 cut(s) 216, 325, 358, 538, 547, 853, 1318, 1573
FbaI TGATCA 1 cut(s) 738
Fnu4HI GCNGC 2 cut(s) 700, 1565
FokI GGATG 2 cut(s) 1004, 1520
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 2 cut(s) 700, 1565
FspBI CTAG 6 cut(s) 47, 366, 684, 755, 1076, 1307
GlaI GCGC 1 cut(s) 1411
GluI GCNGC 2 cut(s) 700, 1565
HaeII RGCGCY 1 cut(s) 1413
HaeIII GGCC 4 cut(s) 45, 171, 749, 1567
HapII CCGG 2 cut(s) 317, 575
HhaI GCGC 1 cut(s) 1412
Hin1II CATG 8 cut(s) 220, 329, 362, 542, 551, 857, 1322, 1577
Hin6I GCGC 1 cut(s) 1410
HinP1I GCGC 1 cut(s) 1410
HindIII AAGCTT 1 cut(s) 64
HpaII CCGG 2 cut(s) 317, 575
HphI GGTGA 1 cut(s) 1129
Hpy166II GTNNAC 3 cut(s) 123, 666, 800
Hpy188I TCNGA 7 cut(s) 129, 167, 303, 608, 673, 832, 938
Hpy8I GTNNAC 3 cut(s) 123, 666, 800
HpyCH4III ACNGT 3 cut(s) 311, 778, 1066
HpyCH4IV ACGT 3 cut(s) 1016, 1125, 1284
HpyCH4V TGCA 6 cut(s) 157, 402, 965, 1011, 1028, 1463
HpyF10VI GCNNNNNNNGC 3 cut(s) 168, 539, 1481
HpyF3I CTNAG 4 cut(s) 300, 372, 393, 594
HpySE526I ACGT 3 cut(s) 1016, 1125, 1284
Hsp92II CATG 8 cut(s) 220, 329, 362, 542, 551, 857, 1322, 1577
HspAI GCGC 1 cut(s) 1410
Kpn2I TCCGGA 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 738
Kzo9I GATC 3 cut(s) 102, 738, 1321
LmnI GCTCC 2 cut(s) 84, 863
Lsp1109I GCAGC 1 cut(s) 711
LweI GCATC 3 cut(s) 171, 801, 952
MaeI CTAG 6 cut(s) 47, 366, 684, 755, 1076, 1307
MaeII ACGT 3 cut(s) 1016, 1125, 1284
MaeIII GTNAC 4 cut(s) 311, 487, 704, 1529
MalI GATC 3 cut(s) 104, 740, 1323
MboI GATC 3 cut(s) 102, 738, 1321
MboII GAAGA 9 cut(s) 27, 79, 123, 341, 475, 629, 662, 1064, 1352
MflI RGATCY 1 cut(s) 102
MhlI GDGCHC 2 cut(s) 81, 373
MluCI AATT 8 cut(s) 228, 247, 343, 455, 518, 766, 1289, 1578
MlyI GAGTC 5 cut(s) 118, 124, 292, 1205, 1337
MmeI TCCRAC 1 cut(s) 152
MroI TCCGGA 1 cut(s) 574
MroXI GAANNNNTTC 1 cut(s) 1293
MseI TTAA 5 cut(s) 12, 330, 414, 560, 762
MslI CAYNNNNRTG 1 cut(s) 960
MspA1I CMGCKG 1 cut(s) 376
MspI CCGG 2 cut(s) 317, 575
MspR9I CCNGG 4 cut(s) 317, 789, 1060, 1527
MvaI CCWGG 3 cut(s) 789, 1060, 1527
MwoI GCNNNNNNNGC 3 cut(s) 168, 539, 1481
NciI CCSGG 1 cut(s) 317
NcoI CCATGG 1 cut(s) 547
NdeII GATC 3 cut(s) 102, 738, 1321
NlaIII CATG 8 cut(s) 220, 329, 362, 542, 551, 857, 1322, 1577
NlaIV GGNNCC 3 cut(s) 786, 859, 1434
NmuCI GTSAC 1 cut(s) 487
NspV TTCGAA 1 cut(s) 109
PaeR7I CTCGAG 1 cut(s) 270
PceI AGGCCT 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PdmI GAANNNNTTC 1 cut(s) 1293
PfeI GAWTC 8 cut(s) 320, 571, 827, 1223, 1294, 1329, 1368, 1495
PflMI CCANNNNNTGG 1 cut(s) 1574
PkrI GCNGC 2 cut(s) 701, 1566
PleI GAGTC 5 cut(s) 118, 124, 292, 1204, 1337
PpsI GAGTC 5 cut(s) 118, 124, 292, 1204, 1337
PpuMI RGGWCCY 1 cut(s) 1433
Psp124BI GAGCTC 1 cut(s) 373
Psp1406I AACGTT 1 cut(s) 1284
Psp5II RGGWCCY 1 cut(s) 1433
Psp6I CCWGG 3 cut(s) 787, 1058, 1525
PspEI GGTNACC 1 cut(s) 311
PspGI CCWGG 3 cut(s) 787, 1058, 1525
PspN4I GGNNCC 3 cut(s) 786, 859, 1434
PspPI GGNCC 2 cut(s) 170, 1433
PspPPI RGGWCCY 1 cut(s) 1433
PstI CTGCAG 1 cut(s) 159
PstNI CAGNNNCTG 1 cut(s) 392
PsuI RGATCY 1 cut(s) 102
PvuII CAGCTG 1 cut(s) 376
RsaI GTAC 1 cut(s) 852
RsaNI GTAC 1 cut(s) 851
RseI CAYNNNNRTG 1 cut(s) 960
SacI GAGCTC 1 cut(s) 373
SaqAI TTAA 5 cut(s) 12, 330, 414, 560, 762
SatI GCNGC 2 cut(s) 700, 1565
Sau3AI GATC 3 cut(s) 102, 738, 1321
Sau96I GGNCC 2 cut(s) 170, 1433
SchI GAGTC 5 cut(s) 118, 124, 292, 1205, 1337
ScrFI CCNGG 4 cut(s) 317, 789, 1060, 1527
SduI GDGCHC 2 cut(s) 81, 373
SfaNI GCATC 3 cut(s) 171, 801, 952
SfcI CTRYAG 1 cut(s) 155
Sfr274I CTCGAG 1 cut(s) 270
SfuI TTCGAA 1 cut(s) 109
SinI GGWCC 1 cut(s) 1433
SlaI CTCGAG 1 cut(s) 270
SmiMI CAYNNNNRTG 1 cut(s) 960
SmlI CTYRAG 2 cut(s) 270, 839
SmoI CTYRAG 2 cut(s) 270, 839
Sse9I AATT 8 cut(s) 228, 247, 343, 455, 518, 766, 1289, 1578
SseBI AGGCCT 1 cut(s) 45
SsiI CCGC 2 cut(s) 523, 1564
SspMI CTAG 6 cut(s) 47, 366, 684, 755, 1076, 1307
SstI GAGCTC 1 cut(s) 373
StuI AGGCCT 1 cut(s) 45
StyD4I CCNGG 4 cut(s) 315, 787, 1058, 1525
StyI CCWWGG 1 cut(s) 547
TaaI ACNGT 3 cut(s) 311, 778, 1066
TaiI ACGT 3 cut(s) 1019, 1128, 1287
TaqI TCGA 5 cut(s) 109, 271, 825, 1327, 1438
TasI AATT 8 cut(s) 228, 247, 343, 455, 518, 766, 1289, 1578
TatI WGTACW 1 cut(s) 850
TauI GCSGC 1 cut(s) 1567
TfiI GAWTC 8 cut(s) 320, 571, 827, 1223, 1294, 1329, 1368, 1495
Tru1I TTAA 5 cut(s) 12, 330, 414, 560, 762
Tru9I TTAA 5 cut(s) 12, 330, 414, 560, 762
TscAI CASTG 1 cut(s) 25
TseFI GTSAC 1 cut(s) 487
TseI GCWGC 1 cut(s) 699
Tsp45I GTSAC 1 cut(s) 487
TspDTI ATGAA 4 cut(s) 236, 248, 1405, 1538
TspGWI ACGGA 2 cut(s) 207, 1103
TspRI CASTG 1 cut(s) 25
Van91I CCANNNNNTGG 1 cut(s) 1574
VpaK11BI GGWCC 1 cut(s) 1433
XapI RAATTY 4 cut(s) 247, 455, 766, 1578
XbaI TCTAGA 2 cut(s) 365, 683
XcmI CCANNNNNNNNNTGG 1 cut(s) 545
XhoI CTCGAG 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 1293
XspI CTAG 6 cut(s) 47, 366, 684, 755, 1076, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.