Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 188 |
| AccB1I | GGYRCC | 1 cut(s) | 188 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1214 |
| AccII | CGCG | 2 cut(s) | 314, 986 |
| AccIII | TCCGGA | 1 cut(s) | 618 |
| AciI | CCGC | 1 cut(s) | 986 |
| AclWI | GGATC | 1 cut(s) | 629 |
| AcsI | RAATTY | 6 cut(s) | 476, 546, 589, 787, 943, 1080 |
| AcuI | CTGAAG | 3 cut(s) | 161, 342, 477 |
| AfaI | GTAC | 3 cut(s) | 190, 415, 662 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1214 |
| AflIII | ACRYGT | 1 cut(s) | 555 |
| AgsI | TTSAA | 5 cut(s) | 67, 131, 866, 997, 1223 |
| AjnI | CCWGG | 2 cut(s) | 506, 610 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 882, 914 |
| AluBI | AGCT | 6 cut(s) | 197, 295, 352, 410, 528, 881 |
| AluI | AGCT | 6 cut(s) | 197, 295, 352, 410, 528, 881 |
| Alw26I | GTCTC | 1 cut(s) | 843 |
| AlwI | GGATC | 1 cut(s) | 629 |
| Ama87I | CYCGRG | 1 cut(s) | 1165 |
| Aor13HI | TCCGGA | 1 cut(s) | 618 |
| AoxI | GGCC | 1 cut(s) | 22 |
| ApeKI | GCWGC | 4 cut(s) | 522, 525, 1049, 1231 |
| ApoI | RAATTY | 6 cut(s) | 476, 546, 589, 787, 943, 1080 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1065, 1097 |
| Asp718I | GGTACC | 1 cut(s) | 188 |
| AspS9I | GGNCC | 3 cut(s) | 23, 702, 1073 |
| AsuC2I | CCSGG | 2 cut(s) | 1166, 1167 |
| AsuHPI | GGTGA | 1 cut(s) | 947 |
| AvaI | CYCGRG | 1 cut(s) | 1165 |
| AvaII | GGWCC | 2 cut(s) | 702, 1073 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 122, 155 |
| BanI | GGYRCC | 1 cut(s) | 188 |
| BauI | CACGAG | 1 cut(s) | 145 |
| BbvI | GCAGC | 4 cut(s) | 534, 537, 1036, 1218 |
| BccI | CCATC | 4 cut(s) | 38, 418, 732, 788 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 442, 476 |
| BciT130I | CCWGG | 2 cut(s) | 508, 612 |
| BciVI | GTATCC | 1 cut(s) | 626 |
| BclI | TGATCA | 2 cut(s) | 643, 825 |
| BcnI | CCSGG | 2 cut(s) | 1166, 1167 |
| BcoDI | GTCTC | 1 cut(s) | 843 |
| BfaI | CTAG | 3 cut(s) | 411, 716, 1077 |
| BfmI | CTRYAG | 2 cut(s) | 8, 123 |
| BfuI | GTATCC | 1 cut(s) | 626 |
| BisI | GCNGC | 4 cut(s) | 523, 526, 1050, 1232 |
| BlsI | GCNGC | 4 cut(s) | 524, 527, 1051, 1233 |
| BmcAI | AGTACT | 1 cut(s) | 415 |
| Bme1390I | CCNGG | 4 cut(s) | 508, 612, 1166, 1167 |
| Bme18I | GGWCC | 2 cut(s) | 702, 1073 |
| BmeT110I | CYCGRG | 1 cut(s) | 1165 |
| BmgT120I | GGNCC | 3 cut(s) | 23, 702, 1073 |
| BmiI | GGNNCC | 4 cut(s) | 190, 216, 396, 1074 |
| BmrFI | CCNGG | 4 cut(s) | 508, 612, 1166, 1167 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1026 |
| BpuEI | CTTGAG | 1 cut(s) | 137 |
| BpuMI | CCSGG | 2 cut(s) | 1166, 1167 |
| BsaJI | CCNNGG | 2 cut(s) | 1164, 1165 |
| BsaWI | WCCGGW | 2 cut(s) | 217, 618 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1209, 1239 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1214 |
| BseAI | TCCGGA | 1 cut(s) | 618 |
| BseBI | CCWGG | 2 cut(s) | 508, 612 |
| BseDI | CCNNGG | 2 cut(s) | 1164, 1165 |
| BseGI | GGATG | 1 cut(s) | 936 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1214 |
| BseMII | CTCAG | 4 cut(s) | 149, 212, 408, 1026 |
| BseRI | GAGGAG | 1 cut(s) | 1173 |
| BseXI | GCAGC | 4 cut(s) | 534, 537, 1036, 1218 |
| BsgI | GTGCAG | 1 cut(s) | 1217 |
| Bsh1236I | CGCG | 2 cut(s) | 314, 986 |
| BshFI | GGCC | 1 cut(s) | 24 |
| BshNI | GGYRCC | 1 cut(s) | 188 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1165 |
| BsiSI | CCGG | 3 cut(s) | 218, 619, 1166 |
| BslFI | GGGAC | 2 cut(s) | 990, 1086 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1214 |
| BsmAI | GTCTC | 1 cut(s) | 843 |
| BsmFI | GGGAC | 2 cut(s) | 990, 1086 |
| BsmI | GAATGC | 1 cut(s) | 1052 |
| BsnI | GGCC | 1 cut(s) | 24 |
| BsoBI | CYCGRG | 1 cut(s) | 1165 |
| Bsp13I | TCCGGA | 1 cut(s) | 618 |
| Bsp143I | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| Bsp68I | TCGCGA | 1 cut(s) | 314 |
| BspACI | CCGC | 1 cut(s) | 986 |
| BspANI | GGCC | 1 cut(s) | 24 |
| BspCNI | CTCAG | 4 cut(s) | 148, 211, 409, 1025 |
| BspEI | TCCGGA | 1 cut(s) | 618 |
| BspFNI | CGCG | 2 cut(s) | 314, 986 |
| BspLI | GGNNCC | 4 cut(s) | 190, 216, 396, 1074 |
| BspMAI | CTGCAG | 1 cut(s) | 12 |
| BspPI | GGATC | 1 cut(s) | 629 |
| BspT107I | GGYRCC | 1 cut(s) | 188 |
| BssECI | CCNNGG | 2 cut(s) | 1164, 1165 |
| BssMI | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| BssSI | CACGAG | 1 cut(s) | 145 |
| Bst2BI | CACGAG | 1 cut(s) | 145 |
| Bst2UI | CCWGG | 2 cut(s) | 508, 612 |
| Bst4CI | ACNGT | 3 cut(s) | 706, 1146, 1201 |
| Bst6I | CTCTTC | 1 cut(s) | 1191 |
| BstC8I | GCNNGC | 1 cut(s) | 354 |
| BstDEI | CTNAG | 8 cut(s) | 135, 198, 417, 842, 1012, 1026, 1035, 1116 |
| BstF5I | GGATG | 1 cut(s) | 936 |
| BstFNI | CGCG | 2 cut(s) | 314, 986 |
| BstKTI | GATC | 5 cut(s) | 462, 603, 624, 646, 828 |
| BstMAI | GTCTC | 1 cut(s) | 843 |
| BstMBI | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 343, 362, 436 |
| BstNI | CCWGG | 2 cut(s) | 508, 612 |
| BstNSI | RCATGY | 1 cut(s) | 559 |
| BstSCI | CCNGG | 4 cut(s) | 506, 610, 1164, 1165 |
| BstSFI | CTRYAG | 2 cut(s) | 8, 123 |
| BstUI | CGCG | 2 cut(s) | 314, 986 |
| BstV1I | GCAGC | 4 cut(s) | 534, 537, 1036, 1218 |
| BsuI | GTATCC | 1 cut(s) | 626 |
| BsuRI | GGCC | 1 cut(s) | 24 |
| BtsCI | GGATG | 1 cut(s) | 936 |
| BtsIMutI | CAGTG | 3 cut(s) | 185, 653, 1107 |
| BtuMI | TCGCGA | 1 cut(s) | 314 |
| Cac8I | GCNNGC | 1 cut(s) | 354 |
| Cfr13I | GGNCC | 3 cut(s) | 23, 702, 1073 |
| Cfr9I | CCCGGG | 1 cut(s) | 1165 |
| CseI | GACGC | 1 cut(s) | 992 |
| Csp6I | GTAC | 3 cut(s) | 189, 414, 661 |
| CviAII | CATG | 7 cut(s) | 70, 85, 362, 556, 959, 1045, 1214 |
| CviQI | GTAC | 3 cut(s) | 189, 414, 661 |
| DdeI | CTNAG | 8 cut(s) | 135, 198, 417, 842, 1012, 1026, 1035, 1116 |
| DpnI | GATC | 5 cut(s) | 461, 602, 623, 645, 827 |
| DpnII | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| DraI | TTTAAA | 1 cut(s) | 384 |
| Eam1104I | CTCTTC | 1 cut(s) | 1191 |
| EarI | CTCTTC | 1 cut(s) | 1191 |
| Eco47I | GGWCC | 2 cut(s) | 702, 1073 |
| Eco57I | CTGAAG | 3 cut(s) | 161, 342, 477 |
| Eco88I | CYCGRG | 1 cut(s) | 1165 |
| EcoO109I | RGGNCCY | 2 cut(s) | 23, 1073 |
| EcoRII | CCWGG | 2 cut(s) | 506, 610 |
| FaeI | CATG | 7 cut(s) | 73, 88, 365, 559, 962, 1048, 1217 |
| FaqI | GGGAC | 2 cut(s) | 990, 1086 |
| FatI | CATG | 7 cut(s) | 69, 84, 361, 555, 958, 1044, 1213 |
| FauNDI | CATATG | 1 cut(s) | 160 |
| FbaI | TGATCA | 2 cut(s) | 643, 825 |
| Fnu4HI | GCNGC | 4 cut(s) | 523, 526, 1050, 1232 |
| FokI | GGATG | 1 cut(s) | 923 |
| Fsp4HI | GCNGC | 4 cut(s) | 523, 526, 1050, 1232 |
| FspBI | CTAG | 3 cut(s) | 411, 716, 1077 |
| GluI | GCNGC | 4 cut(s) | 523, 526, 1050, 1232 |
| HaeIII | GGCC | 1 cut(s) | 24 |
| HapII | CCGG | 3 cut(s) | 218, 619, 1166 |
| HgaI | GACGC | 1 cut(s) | 992 |
| Hin1II | CATG | 7 cut(s) | 73, 88, 365, 559, 962, 1048, 1217 |
| HincII | GTYRAC | 1 cut(s) | 561 |
| HindII | GTYRAC | 1 cut(s) | 561 |
| HinfI | GANTC | 6 cut(s) | 15, 781, 836, 1008, 1135, 1156 |
| HpaII | CCGG | 3 cut(s) | 218, 619, 1166 |
| HphI | GGTGA | 1 cut(s) | 947 |
| Hpy166II | GTNNAC | 3 cut(s) | 489, 561, 1177 |
| Hpy188I | TCNGA | 5 cut(s) | 322, 496, 773, 793, 1015 |
| Hpy8I | GTNNAC | 3 cut(s) | 489, 561, 1177 |
| HpyAV | CCTTC | 6 cut(s) | 36, 70, 317, 376, 530, 1010 |
| HpyCH4III | ACNGT | 3 cut(s) | 706, 1146, 1201 |
| HpyCH4IV | ACGT | 1 cut(s) | 1173 |
| HpyCH4V | TGCA | 9 cut(s) | 10, 228, 265, 430, 651, 763, 1052, 1141, 1234 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 343, 362, 436 |
| HpyF3I | CTNAG | 8 cut(s) | 135, 198, 417, 842, 1012, 1026, 1035, 1116 |
| HpySE526I | ACGT | 1 cut(s) | 1173 |
| Hsp92II | CATG | 7 cut(s) | 73, 88, 365, 559, 962, 1048, 1217 |
| Kpn2I | TCCGGA | 1 cut(s) | 618 |
| KpnI | GGTACC | 1 cut(s) | 192 |
| Ksp22I | TGATCA | 2 cut(s) | 643, 825 |
| Kzo9I | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| LmnI | GCTCC | 1 cut(s) | 1186 |
| Lsp1109I | GCAGC | 4 cut(s) | 534, 537, 1036, 1218 |
| MaeI | CTAG | 3 cut(s) | 411, 716, 1077 |
| MaeII | ACGT | 1 cut(s) | 1173 |
| MaeIII | GTNAC | 1 cut(s) | 1091 |
| MalI | GATC | 5 cut(s) | 461, 602, 623, 645, 827 |
| MboI | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| MboII | GAAGA | 8 cut(s) | 85, 167, 178, 301, 433, 553, 704, 1178 |
| MluCI | AATT | 8 cut(s) | 476, 546, 589, 639, 764, 787, 943, 1080 |
| MlyI | GAGTC | 2 cut(s) | 845, 1150 |
| MnlI | CCTC | 7 cut(s) | 144, 320, 583, 733, 864, 975, 1194 |
| MroI | TCCGGA | 1 cut(s) | 618 |
| MseI | TTAA | 3 cut(s) | 383, 480, 597 |
| MspI | CCGG | 3 cut(s) | 218, 619, 1166 |
| MspR9I | CCNGG | 4 cut(s) | 508, 612, 1166, 1167 |
| Mva1269I | GAATGC | 1 cut(s) | 1052 |
| MvaI | CCWGG | 2 cut(s) | 508, 612 |
| MvnI | CGCG | 2 cut(s) | 314, 986 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 343, 362, 436 |
| NciI | CCSGG | 2 cut(s) | 1166, 1167 |
| NdeI | CATATG | 1 cut(s) | 160 |
| NdeII | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| NlaIII | CATG | 7 cut(s) | 73, 88, 365, 559, 962, 1048, 1217 |
| NlaIV | GGNNCC | 4 cut(s) | 190, 216, 396, 1074 |
| NruI | TCGCGA | 1 cut(s) | 314 |
| NspI | RCATGY | 1 cut(s) | 559 |
| PciI | ACATGT | 1 cut(s) | 555 |
| PctI | GAATGC | 1 cut(s) | 1052 |
| PfeI | GAWTC | 4 cut(s) | 15, 781, 1008, 1135 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1214 |
| PfoI | TCCNGGA | 1 cut(s) | 506 |
| PkrI | GCNGC | 4 cut(s) | 524, 527, 1051, 1233 |
| PleI | GAGTC | 2 cut(s) | 844, 1150 |
| PpsI | GAGTC | 2 cut(s) | 844, 1150 |
| PpuMI | RGGWCCY | 1 cut(s) | 1073 |
| PscI | ACATGT | 1 cut(s) | 555 |
| Psp5II | RGGWCCY | 1 cut(s) | 1073 |
| Psp6I | CCWGG | 2 cut(s) | 506, 610 |
| PspGI | CCWGG | 2 cut(s) | 506, 610 |
| PspN4I | GGNNCC | 4 cut(s) | 190, 216, 396, 1074 |
| PspPI | GGNCC | 3 cut(s) | 23, 702, 1073 |
| PspPPI | RGGWCCY | 1 cut(s) | 1073 |
| PstI | CTGCAG | 1 cut(s) | 12 |
| RruI | TCGCGA | 1 cut(s) | 314 |
| RsaI | GTAC | 3 cut(s) | 190, 415, 662 |
| RsaNI | GTAC | 3 cut(s) | 189, 414, 661 |
| SaqAI | TTAA | 3 cut(s) | 383, 480, 597 |
| SatI | GCNGC | 4 cut(s) | 523, 526, 1050, 1232 |
| Sau3AI | GATC | 5 cut(s) | 459, 600, 621, 643, 825 |
| Sau96I | GGNCC | 3 cut(s) | 23, 702, 1073 |
| ScaI | AGTACT | 1 cut(s) | 415 |
| SchI | GAGTC | 2 cut(s) | 845, 1150 |
| ScrFI | CCNGG | 4 cut(s) | 508, 612, 1166, 1167 |
| SfcI | CTRYAG | 2 cut(s) | 8, 123 |
| SinI | GGWCC | 2 cut(s) | 702, 1073 |
| SmaI | CCCGGG | 1 cut(s) | 1167 |
| SmlI | CTYRAG | 1 cut(s) | 152 |
| SmoI | CTYRAG | 1 cut(s) | 152 |
| Sse9I | AATT | 8 cut(s) | 476, 546, 589, 639, 764, 787, 943, 1080 |
| SsiI | CCGC | 1 cut(s) | 986 |
| SspI | AATATT | 1 cut(s) | 925 |
| SspMI | CTAG | 3 cut(s) | 411, 716, 1077 |
| StyD4I | CCNGG | 4 cut(s) | 506, 610, 1164, 1165 |
| TaaI | ACNGT | 3 cut(s) | 706, 1146, 1201 |
| TaiI | ACGT | 1 cut(s) | 1176 |
| TaqI | TCGA | 2 cut(s) | 42, 462 |
| TasI | AATT | 8 cut(s) | 476, 546, 589, 639, 764, 787, 943, 1080 |
| TatI | WGTACW | 2 cut(s) | 413, 660 |
| TfiI | GAWTC | 4 cut(s) | 15, 781, 1008, 1135 |
| Tru1I | TTAA | 3 cut(s) | 383, 480, 597 |
| Tru9I | TTAA | 3 cut(s) | 383, 480, 597 |
| TscAI | CASTG | 3 cut(s) | 185, 653, 1114 |
| TseI | GCWGC | 4 cut(s) | 522, 525, 1049, 1231 |
| TspDTI | ATGAA | 5 cut(s) | 86, 284, 833, 1045, 1178 |
| TspGWI | ACGGA | 1 cut(s) | 578 |
| TspMI | CCCGGG | 1 cut(s) | 1165 |
| TspRI | CASTG | 3 cut(s) | 185, 653, 1114 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1214 |
| VpaK11BI | GGWCC | 2 cut(s) | 702, 1073 |
| XapI | RAATTY | 6 cut(s) | 476, 546, 589, 787, 943, 1080 |
| XceI | RCATGY | 1 cut(s) | 559 |
| XmaI | CCCGGG | 1 cut(s) | 1165 |
| XspI | CTAG | 3 cut(s) | 411, 716, 1077 |
| ZrmI | AGTACT | 1 cut(s) | 415 |