Rw4G031270

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000078
Physical Location & Seq
Reverse (-)
58157711 .. 58159925
2215 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031270.1

Sequence Viewer

Length: 1557 bp
ATGAGTTCTTTGGGAGGCCTGGTATTCATTCTTTTAAGCTTTCTGTTTTTGTTCCTTCTCTTATTTCTGATCAAGATCTTTCACAAACTATGGTGGAATCCGACTCGTATACAGAAGCTGATGGCTTTGCAAGGAATCAAAGGTCCTTCTTACAGATTTTTCCATGGAAATGCCAAAGAAATCTCCAGCATGCAAAAAGAAGCCATGAGCAGGCCCAAAAGTTTGTCACATGATATATTTTCTCATGTTCAACCTCATTACTACTTATGGAGCGGGGTATATGGGAAGAATTTTCTTCAATGGCATGGTTCTCGAGCTCAATTGGTCATTACTGAACCTGAGTTGTGCAAAGAGGTACTAAATAACAAAGATAGAACTTTCCTGAAAACCGATACCCCAGTATTTGTAAAGAAGCTATTGGGAGATGGACTTGTTACAACTGAAGGTGAAAAATGGGGAAGATTGCGAAAGCTGGCCAACTCTGCCTTCCATGGAGAGAGCTTAAAGAGTCTGATTCCTGCAACTATAGCTAGTGCTGAGTTGATGCTAGAGAGATGGAAAAATCATGAAGAAGAGAAAGAGATTGAGATATTTGAAGAATTCAGGTTGTTGACATCAGAAGTGATTTCCAGAACAGCATTTGGTAGCAGCTATTCAGAAGGGAAGAGCATTTTTGACATGTTGATGAAGTTAGCCTTCTTAACATTCAAAAATGCTTTCAAAACTAAGTTTCCTATCATCAGTAACATTTTCAAAACCGGTGATGATTTTGAAGCAGAGAAGCTTGAGAAAGGAGTACGCGACTCCATAATAGAGATAGTCAAGAAAAGAGAACAGAAGGCAATGACCATGAGGGAGGAAGAAAAAAGTTTTGGGAGTGATTTTCTTGGATTACTTTTGAAGGCTCATCATGATGTCGATAACAACCGGAGGATTACAGTGGATGATCTAGTTGATGAGTGCAAGACATTTTACTTTGCTGGACAAGAAACCACCAACAGTCTGCTAGCTTGGACTGTGTTTCTTCTGGCACTCCATACAGATTGGCAAGAGGAAGCAAGAAAAGAAGTCCTGCAATTATTCGGCAAACAAAATCCAAACCCTGATGACATTTCCAAACTAAAAACAATGGGTATGATCATCAATGAGTCTCTAAGGTTATATCCTCCTGTTGTGTCCCTCGAGAGGAAAGTCGCACGAGAAGTTAGACTCGGAAAACTCCTTGTTCCAGCTGATATTGAAATCATTATTCCATGTTTGGCCCTTCAGCATGAGCCTCAATTCTGGGGACAAGATGTGGAATTGTTCAAACCAGAGAGATTCTCAGAAGGGGTTGCTAAAGCTACCAACAATAACATGGCCACTTTTATACCCTTTGGAATGGGACCTAGAAATTGTGTGGGATACAACTTTGCAACCACTGAAGCCAAAATTGCTCTGGCAATGATTCTACAACGCTACACCTTCACACTTTCCCCGGGTTATGTTCACTCGCCCTTTCGGCTTCTTACAGTTCGGCCACAACATGGAGTTCAAGTAATGGTACACTCACTATGA

Protein Analysis

518

Amino Acids

59.42

Weight (kDa)

8.85

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 93 - 494 2.1e-78 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000078)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g15240 FvH4_3g15270 FvH4_3g15280 FvH4_3g32750 FvH4_3g32760 FvH4_3g32760 FvH4_3g32880 FvH4_4g08190 FvH4_4g29160 FvH4_4g29370 FvH4_4g29400 FvH4_4g29420 FvH4_4g29420 FvH4_4g29420 FvH4_4g29430 FvH4_4g29440 FvH4_4g29450 FvH4_4g29450 FvH4_4g29450 FvH4_4g29460 FvH4_4g29461 FvH4_4g29462 FvH4_4g29463 FvH4_4g29480 FvH4_4g29481 FvH4_4g29482 FvH4_4g29483 FvH4_4g29484 FvH4_6g26720 FvH4_6g26720
malus_domestica MD00G1065200.v1.1 MD06G1145800.v1.1 MD06G1146000.v1.1 MD06G1146700.v1.1 MD13G1031000.v1.1 MD13G1033900.v1.1 MD13G1057000.v1.1 MD13G1057100.v1.1 MD13G1057200.v1.1 MD13G1057400.v1.1 MD13G1057800.v1.1 MD13G1058000.v1.1 MD13G1058100.v1.1 MD13G1058200.v1.1 MD13G1058300.v1.1 MD13G1058500.v1.1 MD13G1058600.v1.1 MD13G1058700.v1.1 MD16G1035700.v1.1 MD16G1056400.v1.1 MD16G1056600.v1.1 MD16G1056700.v1.1 MD16G1056900.v1.1 MD16G1058100.v1.1
prunus_persica Prupe.1G291100_v2.0.a1 Prupe.1G291200_v2.0.a1 Prupe.1G291500_v2.0.a1 Prupe.1G291600_v2.0.a1 Prupe.1G291700_v2.0.a1 Prupe.1G291800_v2.0.a1 Prupe.1G291900_v2.0.a1 Prupe.1G292000_v2.0.a1 Prupe.1G292100_v2.0.a1 Prupe.1G292400_v2.0.a1 Prupe.1G292500_v2.0.a1 Prupe.1G292600_v2.0.a1 Prupe.1G292700_v2.0.a1 Prupe.1G292800_v2.0.a1 Prupe.1G296000_v2.0.a1 Prupe.1G296100_v2.0.a1 Prupe.1G296300_v2.0.a1 Prupe.1G296600_v2.0.a1 Prupe.1G296900_v2.0.a1 Prupe.1G534600_v2.0.a1 Prupe.5G046700_v2.0.a1 Prupe.8G021800_v2.0.a1 Prupe.8G026400_v2.0.a1
pyrus_communis pycom06g13510 pycom06g13530 pycom06g13540 pycom06g13570 pycom06g13590 pycom06g13620 pycom13g02710 pycom13g02930 pycom13g05100 pycom13g05110 pycom13g05140 pycom13g05160 pycom13g05170 pycom13g05190 pycom13g05200 pycom13g05210 pycom13g05220 pycom16g03060 pycom16g04940 pycom16g04960 pycom16g04990 pycom16g05000 pycom16g05080
rosa_chinensis RchiOBHm_Chr1g0337981 RchiOBHm_Chr1g0337991 RchiOBHm_Chr1g0339431 RchiOBHm_Chr4g0402401 RchiOBHm_Chr4g0420571 RchiOBHm_Chr4g0420601 RchiOBHm_Chr4g0420611 RchiOBHm_Chr4g0420621 RchiOBHm_Chr4g0420631 RchiOBHm_Chr4g0437211 RchiOBHm_Chr4g0437221 RchiOBHm_Chr4g0437251 RchiOBHm_Chr4g0437431 RchiOBHm_Chr4g0437441 RchiOBHm_Chr4g0437491 RchiOBHm_Chr4g0437501 RchiOBHm_Chr4g0437511 RchiOBHm_Chr4g0437521 RchiOBHm_Chr4g0437531 RchiOBHm_Chr4g0437541 RchiOBHm_Chr4g0437551 RchiOBHm_Chr4g0437591 RchiOBHm_Chr4g0437631 RchiOBHm_Chr4g0437641 RchiOBHm_Chr4g0437671 RchiOBHm_Chr4g0437681 RchiOBHm_Chr4g0437711 RchiOBHm_Chr4g0437731 RchiOBHm_Chr4g0437741 RchiOBHm_Chr4g0437751 RchiOBHm_Chr5g0025681 RchiOBHm_Chr5g0025711 RchiOBHm_Chr5g0025731 RchiOBHm_Chr5g0059841 RchiOBHm_Chr6g0272651
rosa_laevigata RLG00000006420 RLG00000006421 RLG00000006423 RLG00000006426 RLG00000006427 RLG00000006428 RLG00000006429 RLG00000006431 RLG00000006433 RLG00000006434 RLG00000006435 RLG00000006436 RLG00000006437 RLG00000006455 RLG00000007746 RLG00000007747 RLG00000007748 RLG00000007749 RLG00000007750 RLG00000009091 RLG00000009092 RLG00000009095 RLG00000009096 RLG00000017856 RLG00000028094 RLG00000029230 RLG00000029231 RLG00000030008 RLG00000032859 RLG00000032860 RLG00000032861 RLG00000032865 RLG00000032868 RLG00000035332
rosa_multiflora Rmu_co7986016.1_g000001 Rmu_co8226539.1_g000001 Rmu_co8349613.1_g000001 Rmu_sc0000272.1_g000014 Rmu_sc0000591.1_g000003 Rmu_sc0000613.1_g000013 Rmu_sc0001629.1_g000022 Rmu_sc0001629.1_g000025 Rmu_sc0002131.1_g000027 Rmu_sc0002137.1_g000006 Rmu_sc0002137.1_g000014 Rmu_sc0002137.1_g000019 Rmu_sc0002472.1_g000002 Rmu_sc0002472.1_g000012 Rmu_sc0002472.1_g000018 Rmu_sc0002472.1_g000034 Rmu_sc0002472.1_g000040 Rmu_sc0002472.1_g000051 Rmu_sc0002638.1_g000005 Rmu_sc0002638.1_g000010 Rmu_sc0002638.1_g000025 Rmu_sc0002638.1_g000030 Rmu_sc0002638.1_g000037 Rmu_sc0002638.1_g000042 Rmu_sc0004339.1_g000019 Rmu_sc0005580.1_g000013 Rmu_sc0006617.1_g000002 Rmu_sc0006986.1_g000003 Rmu_sc0006986.1_g000017 Rmu_sc0008352.1_g000001 Rmu_sc0008352.1_g000002 Rmu_sc0009431.1_g000011 Rmu_sc0011080.1_g000003 Rmu_sc0011080.1_g000006 Rmu_sc0016549.1_g000001 Rmu_sc0016549.1_g000002 Rmu_sc0017154.1_g000001 Rmu_sc0017155.1_g000001 Rmu_sc0028438.1_g000001 Rmu_sc0041413.1_g000001 Rmu_ssc0000330.1_g000022
rosa_roxburghii Rroxscaffold_1G00020750 Rroxscaffold_1G00053460 Rroxscaffold_1G00054250 Rroxscaffold_1G00054260 Rroxscaffold_1G00054270 Rroxscaffold_1G00054290 Rroxscaffold_2G00120210 Rroxscaffold_4G00313460 Rroxscaffold_4G00313470 Rroxscaffold_4G00330630 Rroxscaffold_5G00347070 Rroxscaffold_5G00347110 Rroxscaffold_5G00378670 Rroxscaffold_5G00378690 Rroxscaffold_5G00378720 Rroxscaffold_5G00378730 Rroxscaffold_5G00378740 Rroxscaffold_5G00378760 Rroxscaffold_5G00378780 Rroxscaffold_5G00378790 Rroxscaffold_5G00378800 Rroxscaffold_5G00378810 Rroxscaffold_5G00378820 Rroxscaffold_5G00378840 Rroxscaffold_5G00378860 Rroxscaffold_5G00378870 Rroxscaffold_7G00196060
rosa_rugosa Rorug01G0142200.1 Rorug01G0332800 Rorug02G0012200 Rorug02G0248300 Rorug03G0219000 Rorug03G0219100 Rorug03G0219200 Rorug04G0035100 Rorug04G0301500 Rorug04G0301500 Rorug04G0301700 Rorug04G0301800 Rorug04G0301800 Rorug04G0301900 Rorug04G0302000 Rorug04G0302500 Rorug04G0302600 Rorug04G0302700 Rorug04G0302800 Rorug04G0302900 Rorug04G0303000 Rorug04G0303000 Rorug04G0303100 Rorug04G0303200 Rorug04G0303300 Rorug04G0303400 Rorug04G0303500 Rorug04G0303500 Rorug05G0087800 Rorug05G0088200 Rorug05G0326300 Rorug06G0173000
rosa_samantha Rh2AG222400 Rh4DG224800 Rh4DG359900 Rh4DG360000 Rh4DG360100 Rh4DG360200 Rh4DG360300 Rh4DG360500 Rh4DG360700 Rh4DG360800 Rh4DG360900 Rh4DG361000 Rh5DG177900 Rh5DG178200 Rh5DG178400 Rh6CG187800
rosa_wichuraiana Rw1G013580 Rw4G009020 Rw4G019580 Rw4G030990 Rw4G031000 Rw4G031220 Rw4G031230 Rw4G031240 Rw4G031250 Rw4G031260 Rw4G031270 Rw4G031280 Rw4G031290 Rw4G031310 Rw4G031320 Rw4G031330 Rw4G031340 Rw4G031360 Rw4G031370 Rw5G016310 Rw5G016320 Rw5G016330 Rw5G036760 Rw6G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1526
AccBSI CCGCTC 1 cut(s) 273
AccI GTMKAC 1 cut(s) 109
AccII CGCG 1 cut(s) 801
AciI CCGC 1 cut(s) 273
AcoI YGGCCR 3 cut(s) 474, 1359, 1517
AcsI RAATTY 2 cut(s) 289, 599
AcuI CTGAAG 3 cut(s) 462, 1250, 1443
AfaI GTAC 3 cut(s) 357, 798, 1545
AfiI CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
AflIII ACRYGT 1 cut(s) 678
AgeI ACCGGT 1 cut(s) 758
AjnI CCWGG 1 cut(s) 18
AjuI GAANNNNNNNTTGG 4 cut(s) 470, 502, 855, 887
Alw21I GWGCWC 1 cut(s) 319
Alw26I GTCTC 1 cut(s) 1155
AlwNI CAGNNNCTG 1 cut(s) 118
Ama87I CYCGRG 3 cut(s) 312, 1181, 1477
AoxI GGCC 6 cut(s) 16, 212, 474, 1260, 1359, 1517
ApeKI GCWGC 1 cut(s) 648
ApoI RAATTY 2 cut(s) 289, 599
AsiGI ACCGGT 1 cut(s) 758
AspS9I GGNCC 4 cut(s) 143, 213, 1261, 1385
AsuC2I CCSGG 2 cut(s) 1478, 1479
AsuHPI GGTGA 2 cut(s) 458, 773
AsuNHI GCTAGC 1 cut(s) 1006
AvaI CYCGRG 3 cut(s) 312, 1181, 1477
AvaII GGWCC 2 cut(s) 143, 1385
BaeI ACNNNNGTAYC 2 cut(s) 384, 417
BalI TGGCCA 2 cut(s) 476, 1361
BanII GRGCYC 1 cut(s) 319
BauI CACGAG 1 cut(s) 1197
Bbv12I GWGCWC 1 cut(s) 319
BbvI GCAGC 1 cut(s) 660
BccI CCATC 3 cut(s) 115, 419, 549
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BciT130I CCWGG 1 cut(s) 20
BciVI GTATCC 1 cut(s) 1397
BclI TGATCA 2 cut(s) 69, 1137
BcnI CCSGG 2 cut(s) 1478, 1479
BcoDI GTCTC 1 cut(s) 1155
BfaI CTAG 5 cut(s) 531, 548, 950, 1007, 1389
BfmI CTRYAG 1 cut(s) 525
BfuI GTATCC 1 cut(s) 1397
BglI GCCNNNNNGGC 1 cut(s) 1501
BglII AGATCT 1 cut(s) 75
BisI GCNGC 1 cut(s) 649
BlsI GCNGC 1 cut(s) 650
Bme1390I CCNGG 3 cut(s) 20, 1478, 1479
Bme18I GGWCC 2 cut(s) 143, 1385
BmeT110I CYCGRG 3 cut(s) 312, 1181, 1477
BmgT120I GGNCC 4 cut(s) 143, 213, 1261, 1385
BmiI GGNNCC 1 cut(s) 1386
BmrFI CCNGG 3 cut(s) 20, 1478, 1479
BmrI ACTGGG 1 cut(s) 392
BmsI GCATC 1 cut(s) 534
BmtI GCTAGC 1 cut(s) 1010
BmuI ACTGGG 1 cut(s) 392
BplI GAGNNNNNCTC 2 cut(s) 1307, 1339
BpmI CTGGAG 1 cut(s) 169
BpuEI CTTGAG 1 cut(s) 806
BpuMI CCSGG 2 cut(s) 1478, 1479
BsaBI GATNNNNATC 1 cut(s) 74
BsaJI CCNNGG 4 cut(s) 163, 490, 1476, 1477
BsaWI WCCGGW 2 cut(s) 758, 927
BsaXI ACNNNNNCTCC 4 cut(s) 6, 36, 1521, 1551
Bsc4I CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
Bse118I RCCGGY 1 cut(s) 758
Bse1I ACTGG 1 cut(s) 398
Bse3DI GCAATG 2 cut(s) 849, 1449
Bse8I GATNNNNATC 1 cut(s) 74
BseBI CCWGG 1 cut(s) 20
BseDI CCNNGG 4 cut(s) 163, 490, 1476, 1477
BseGI GGATG 1 cut(s) 949
BseJI GATNNNNATC 1 cut(s) 74
BseLI CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
BseMI GCAATG 2 cut(s) 849, 1449
BseMII CTCAG 3 cut(s) 330, 528, 1338
BseNI ACTGG 1 cut(s) 398
BseXI GCAGC 1 cut(s) 660
Bsh1236I CGCG 1 cut(s) 801
BshFI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BshTI ACCGGT 1 cut(s) 758
BsiHKAI GWGCWC 1 cut(s) 319
BsiHKCI CYCGRG 3 cut(s) 312, 1181, 1477
BsiSI CCGG 3 cut(s) 759, 928, 1478
BslFI GGGAC 3 cut(s) 1162, 1302, 1398
BslI CCNNNNNNNGG 3 cut(s) 210, 1185, 1526
BsmAI GTCTC 1 cut(s) 1155
BsmFI GGGAC 3 cut(s) 1162, 1302, 1398
BsnI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BsoBI CYCGRG 3 cut(s) 312, 1181, 1477
Bsp1286I GDGCHC 1 cut(s) 319
Bsp143I GATC 4 cut(s) 69, 75, 946, 1137
Bsp19I CCATGG 2 cut(s) 163, 490
BspACI CCGC 1 cut(s) 273
BspANI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BspCNI CTCAG 3 cut(s) 331, 529, 1337
BspFNI CGCG 1 cut(s) 801
BspHI TCATGA 2 cut(s) 565, 910
BspLI GGNNCC 1 cut(s) 1386
BspOI GCTAGC 1 cut(s) 1010
BspQI GCTCTTC 1 cut(s) 659
BsrBI CCGCTC 1 cut(s) 273
BsrDI GCAATG 2 cut(s) 849, 1449
BsrFI RCCGGY 1 cut(s) 758
BsrI ACTGG 1 cut(s) 398
BssAI RCCGGY 1 cut(s) 758
BssECI CCNNGG 4 cut(s) 163, 490, 1476, 1477
BssMI GATC 4 cut(s) 69, 75, 946, 1137
BssNAI GTATAC 1 cut(s) 110
BssSI CACGAG 1 cut(s) 1197
BssT1I CCWWGG 2 cut(s) 163, 490
Bst1107I GTATAC 1 cut(s) 110
Bst2BI CACGAG 1 cut(s) 1197
Bst2UI CCWGG 1 cut(s) 20
Bst4CI ACNGT 4 cut(s) 940, 1001, 1018, 1513
Bst6I CTCTTC 2 cut(s) 567, 659
BstC8I GCNNGC 4 cut(s) 191, 212, 474, 1008
BstDEI CTNAG 5 cut(s) 339, 537, 726, 1154, 1324
BstDSI CCRYGG 2 cut(s) 163, 490
BstF5I GGATG 1 cut(s) 949
BstFNI CGCG 1 cut(s) 801
BstKTI GATC 4 cut(s) 72, 78, 949, 1140
BstMAI GTCTC 1 cut(s) 1155
BstMBI GATC 4 cut(s) 69, 75, 946, 1137
BstMWI GCNNNNNNNGC 4 cut(s) 482, 527, 1433, 1501
BstNI CCWGG 1 cut(s) 20
BstNSI RCATGY 2 cut(s) 193, 682
BstSCI CCNGG 3 cut(s) 18, 1476, 1477
BstSFI CTRYAG 1 cut(s) 525
BstUI CGCG 1 cut(s) 801
BstV1I GCAGC 1 cut(s) 660
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BstZ17I GTATAC 1 cut(s) 110
BsuI GTATCC 1 cut(s) 1397
BsuRI GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
BtgI CCRYGG 2 cut(s) 163, 490
BtsCI GGATG 1 cut(s) 949
BtsIMutI CAGTG 2 cut(s) 945, 1419
Cac8I GCNNGC 4 cut(s) 191, 212, 474, 1008
CaiI CAGNNNCTG 1 cut(s) 118
CciI TCATGA 2 cut(s) 565, 910
Cfr10I RCCGGY 1 cut(s) 758
Cfr13I GGNCC 4 cut(s) 143, 213, 1261, 1385
Cfr9I CCCGGG 1 cut(s) 1477
Csp6I GTAC 3 cut(s) 356, 797, 1544
CspAI ACCGGT 1 cut(s) 758
CviQI GTAC 3 cut(s) 356, 797, 1544
DdeI CTNAG 5 cut(s) 339, 537, 726, 1154, 1324
DpnI GATC 4 cut(s) 71, 77, 948, 1139
DpnII GATC 4 cut(s) 69, 75, 946, 1137
EaeI YGGCCR 3 cut(s) 474, 1359, 1517
Eam1104I CTCTTC 2 cut(s) 567, 659
EarI CTCTTC 2 cut(s) 567, 659
Ecl136II GAGCTC 1 cut(s) 317
Eco130I CCWWGG 2 cut(s) 163, 490
Eco147I AGGCCT 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 319
Eco47I GGWCC 2 cut(s) 143, 1385
Eco53kI GAGCTC 1 cut(s) 317
Eco57I CTGAAG 3 cut(s) 462, 1250, 1443
Eco88I CYCGRG 3 cut(s) 312, 1181, 1477
EcoICRI GAGCTC 1 cut(s) 317
EcoO109I RGGNCCY 2 cut(s) 143, 1385
EcoRI GAATTC 1 cut(s) 599
EcoRII CCWGG 1 cut(s) 18
EcoT14I CCWWGG 2 cut(s) 163, 490
EcoT38I GRGCYC 1 cut(s) 319
ErhI CCWWGG 2 cut(s) 163, 490
FalI AAGNNNNNCTT 2 cut(s) 680, 712
FaqI GGGAC 3 cut(s) 1162, 1302, 1398
FauI CCCGC 1 cut(s) 266
FbaI TGATCA 2 cut(s) 69, 1137
FblI GTMKAC 1 cut(s) 109
Fnu4HI GCNGC 1 cut(s) 649
FokI GGATG 1 cut(s) 956
FriOI GRGCYC 1 cut(s) 319
Fsp4HI GCNGC 1 cut(s) 649
FspBI CTAG 5 cut(s) 531, 548, 950, 1007, 1389
GluI GCNGC 1 cut(s) 649
GsuI CTGGAG 1 cut(s) 169
HaeIII GGCC 6 cut(s) 18, 214, 476, 1262, 1361, 1519
HapII CCGG 3 cut(s) 759, 928, 1478
HincII GTYRAC 1 cut(s) 612
HindII GTYRAC 1 cut(s) 612
HindIII AAGCTT 2 cut(s) 37, 782
HpaII CCGG 3 cut(s) 759, 928, 1478
HphI GGTGA 2 cut(s) 458, 773
Hpy166II GTNNAC 4 cut(s) 110, 612, 1489, 1546
Hpy188I TCNGA 7 cut(s) 69, 102, 513, 619, 658, 1214, 1327
Hpy188III TCNNGA 8 cut(s) 73, 312, 382, 566, 630, 823, 911, 1183
Hpy8I GTNNAC 4 cut(s) 110, 612, 1489, 1546
HpyCH4III ACNGT 4 cut(s) 940, 1001, 1018, 1513
HpyCH4V TGCA 7 cut(s) 130, 193, 348, 521, 963, 1075, 1415
HpyF10VI GCNNNNNNNGC 4 cut(s) 482, 527, 1433, 1501
HpyF3I CTNAG 5 cut(s) 339, 537, 726, 1154, 1324
Ksp22I TGATCA 2 cut(s) 69, 1137
Kzo9I GATC 4 cut(s) 69, 75, 946, 1137
LguI GCTCTTC 1 cut(s) 659
LmnI GCTCC 1 cut(s) 270
Lsp1109I GCAGC 1 cut(s) 660
LweI GCATC 1 cut(s) 534
MaeI CTAG 5 cut(s) 531, 548, 950, 1007, 1389
MaeIII GTNAC 3 cut(s) 225, 433, 743
MalI GATC 4 cut(s) 71, 77, 948, 1139
MbiI CCGCTC 1 cut(s) 273
MboI GATC 4 cut(s) 69, 75, 946, 1137
MboII GAAGA 9 cut(s) 287, 298, 471, 581, 584, 608, 676, 872, 1016
MfeI CAATTG 1 cut(s) 320
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 1 cut(s) 319
MlsI TGGCCA 2 cut(s) 476, 1361
MluCI AATT 8 cut(s) 289, 320, 599, 1076, 1280, 1301, 1393, 1431
MluNI TGGCCA 2 cut(s) 476, 1361
MlyI GAGTC 5 cut(s) 97, 517, 797, 1157, 1203
MmeI TCCRAC 1 cut(s) 125
Mox20I TGGCCA 2 cut(s) 476, 1361
MscI TGGCCA 2 cut(s) 476, 1361
MseI TTAA 3 cut(s) 35, 503, 701
MslI CAYNNNNRTG 3 cut(s) 168, 683, 912
Msp20I TGGCCA 2 cut(s) 476, 1361
MspA1I CMGCKG 1 cut(s) 1232
MspI CCGG 3 cut(s) 759, 928, 1478
MspR9I CCNGG 3 cut(s) 20, 1478, 1479
MunI CAATTG 1 cut(s) 320
MvaI CCWGG 1 cut(s) 20
MvnI CGCG 1 cut(s) 801
MwoI GCNNNNNNNGC 4 cut(s) 482, 527, 1433, 1501
NciI CCSGG 2 cut(s) 1478, 1479
NcoI CCATGG 2 cut(s) 163, 490
NdeII GATC 4 cut(s) 69, 75, 946, 1137
NheI GCTAGC 1 cut(s) 1006
NlaIV GGNNCC 1 cut(s) 1386
NmuCI GTSAC 1 cut(s) 225
NspI RCATGY 2 cut(s) 193, 682
PaeI GCATGC 1 cut(s) 193
PaeR7I CTCGAG 2 cut(s) 312, 1181
PagI TCATGA 2 cut(s) 565, 910
PceI AGGCCT 1 cut(s) 18
PciI ACATGT 1 cut(s) 678
PciSI GCTCTTC 1 cut(s) 659
PfeI GAWTC 5 cut(s) 97, 135, 514, 1320, 1447
PflMI CCANNNNNTGG 1 cut(s) 1526
PinAI ACCGGT 1 cut(s) 758
PkrI GCNGC 1 cut(s) 650
PleI GAGTC 5 cut(s) 97, 516, 797, 1156, 1203
PpsI GAGTC 5 cut(s) 97, 516, 797, 1156, 1203
PpuMI RGGWCCY 2 cut(s) 143, 1385
PscI ACATGT 1 cut(s) 678
Psp124BI GAGCTC 1 cut(s) 319
Psp5II RGGWCCY 2 cut(s) 143, 1385
Psp6I CCWGG 1 cut(s) 18
PspGI CCWGG 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 1386
PspPI GGNCC 4 cut(s) 143, 213, 1261, 1385
PspPPI RGGWCCY 2 cut(s) 143, 1385
PstNI CAGNNNCTG 1 cut(s) 118
PsuI RGATCY 1 cut(s) 75
PvuII CAGCTG 1 cut(s) 1232
RsaI GTAC 3 cut(s) 357, 798, 1545
RsaNI GTAC 3 cut(s) 356, 797, 1544
RseI CAYNNNNRTG 3 cut(s) 168, 683, 912
SacI GAGCTC 1 cut(s) 319
SapI GCTCTTC 1 cut(s) 659
SaqAI TTAA 3 cut(s) 35, 503, 701
SatI GCNGC 1 cut(s) 649
Sau3AI GATC 4 cut(s) 69, 75, 946, 1137
Sau96I GGNCC 4 cut(s) 143, 213, 1261, 1385
SchI GAGTC 5 cut(s) 97, 517, 797, 1157, 1203
ScrFI CCNGG 3 cut(s) 20, 1478, 1479
SduI GDGCHC 1 cut(s) 319
SfaNI GCATC 1 cut(s) 534
SfcI CTRYAG 1 cut(s) 525
Sfr274I CTCGAG 2 cut(s) 312, 1181
SinI GGWCC 2 cut(s) 143, 1385
SlaI CTCGAG 2 cut(s) 312, 1181
SmaI CCCGGG 1 cut(s) 1479
SmiMI CAYNNNNRTG 3 cut(s) 168, 683, 912
SmlI CTYRAG 3 cut(s) 312, 785, 1181
SmoI CTYRAG 3 cut(s) 312, 785, 1181
SphI GCATGC 1 cut(s) 193
Sse9I AATT 8 cut(s) 289, 320, 599, 1076, 1280, 1301, 1393, 1431
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 1 cut(s) 273
SspMI CTAG 5 cut(s) 531, 548, 950, 1007, 1389
SstI GAGCTC 1 cut(s) 319
StuI AGGCCT 1 cut(s) 18
StyD4I CCNGG 3 cut(s) 18, 1476, 1477
StyI CCWWGG 2 cut(s) 163, 490
TaaI ACNGT 4 cut(s) 940, 1001, 1018, 1513
TaqI TCGA 3 cut(s) 313, 918, 1182
TasI AATT 8 cut(s) 289, 320, 599, 1076, 1280, 1301, 1393, 1431
TfiI GAWTC 5 cut(s) 97, 135, 514, 1320, 1447
Tru1I TTAA 3 cut(s) 35, 503, 701
Tru9I TTAA 3 cut(s) 35, 503, 701
TscAI CASTG 2 cut(s) 945, 1426
TseFI GTSAC 1 cut(s) 225
TseI GCWGC 1 cut(s) 648
Tsp45I GTSAC 1 cut(s) 225
TspDTI ATGAA 3 cut(s) 16, 582, 701
TspMI CCCGGG 1 cut(s) 1477
TspRI CASTG 2 cut(s) 945, 1426
Van91I CCANNNNNTGG 1 cut(s) 1526
VpaK11BI GGWCC 2 cut(s) 143, 1385
XapI RAATTY 2 cut(s) 289, 599
XceI RCATGY 2 cut(s) 193, 682
XcmI CCANNNNNNNNNTGG 2 cut(s) 1354, 1435
XhoI CTCGAG 2 cut(s) 312, 1181
XmaI CCCGGG 1 cut(s) 1477
XmiI GTMKAC 1 cut(s) 109
XspI CTAG 5 cut(s) 531, 548, 950, 1007, 1389
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.