Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1526 |
| AccBSI | CCGCTC | 1 cut(s) | 782 |
| AccIII | TCCGGA | 1 cut(s) | 1039 |
| AciI | CCGC | 3 cut(s) | 341, 642, 782 |
| AclI | AACGTT | 1 cut(s) | 968 |
| AclWI | GGATC | 2 cut(s) | 92, 1268 |
| AcoI | YGGCCR | 2 cut(s) | 483, 1517 |
| AcsI | RAATTY | 5 cut(s) | 125, 298, 605, 675, 1280 |
| AcuI | CTGAAG | 2 cut(s) | 606, 920 |
| AdeI | CACNNNGTG | 1 cut(s) | 1526 |
| AfaI | GTAC | 2 cut(s) | 319, 894 |
| AfeI | AGCGCT | 1 cut(s) | 1457 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1185, 1526 |
| AflIII | ACRYGT | 2 cut(s) | 231, 1394 |
| AgsI | TTSAA | 8 cut(s) | 260, 308, 560, 602, 636, 1196, 1309, 1535 |
| AjiI | CACGTC | 2 cut(s) | 1249, 1297 |
| AjnI | CCWGG | 4 cut(s) | 731, 739, 1026, 1477 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 479, 511 |
| Alw26I | GTCTC | 4 cut(s) | 12, 40, 542, 1155 |
| AlwI | GGATC | 2 cut(s) | 92, 1268 |
| Aor13HI | TCCGGA | 1 cut(s) | 1039 |
| Aor51HI | AGCGCT | 1 cut(s) | 1457 |
| AoxI | GGCC | 6 cut(s) | 151, 221, 483, 728, 846, 1517 |
| ApeKI | GCWGC | 2 cut(s) | 651, 654 |
| ApoI | RAATTY | 5 cut(s) | 125, 298, 605, 675, 1280 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1304 |
| AspLEI | GCGC | 1 cut(s) | 1458 |
| AspS9I | GGNCC | 5 cut(s) | 152, 279, 639, 729, 1385 |
| AsuHPI | GGTGA | 4 cut(s) | 263, 467, 1459, 1485 |
| AsuII | TTCGAA | 1 cut(s) | 391 |
| AvaII | GGWCC | 3 cut(s) | 279, 639, 1385 |
| BalI | TGGCCA | 1 cut(s) | 485 |
| BanII | GRGCYC | 1 cut(s) | 1221 |
| BbsI | GAAGAC | 1 cut(s) | 1299 |
| BbvI | GCAGC | 2 cut(s) | 663, 666 |
| BccI | CCATC | 3 cut(s) | 685, 1044, 1100 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 571, 605, 1393, 1427 |
| BciT130I | CCWGG | 4 cut(s) | 733, 741, 1028, 1479 |
| BclI | TGATCA | 1 cut(s) | 1137 |
| BcoDI | GTCTC | 4 cut(s) | 12, 40, 542, 1155 |
| BfaI | CTAG | 1 cut(s) | 540 |
| BfmI | CTRYAG | 3 cut(s) | 252, 1451, 1459 |
| BfoI | RGCGCY | 1 cut(s) | 1459 |
| BisI | GCNGC | 2 cut(s) | 652, 655 |
| BlsI | GCNGC | 2 cut(s) | 653, 656 |
| BmcAI | AGTACT | 1 cut(s) | 894 |
| Bme1390I | CCNGG | 4 cut(s) | 733, 741, 1028, 1479 |
| Bme18I | GGWCC | 3 cut(s) | 279, 639, 1385 |
| BmgBI | CACGTC | 2 cut(s) | 1249, 1297 |
| BmgT120I | GGNCC | 5 cut(s) | 152, 279, 639, 729, 1385 |
| BmiI | GGNNCC | 4 cut(s) | 108, 345, 525, 1386 |
| BmrFI | CCNGG | 4 cut(s) | 733, 741, 1028, 1479 |
| BmsI | GCATC | 1 cut(s) | 753 |
| BpiI | GAAGAC | 1 cut(s) | 1299 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 686, 718, 1307, 1339 |
| Bpu14I | TTCGAA | 1 cut(s) | 391 |
| BpuEI | CTTGAG | 2 cut(s) | 22, 812 |
| BsaAI | YACGTR | 1 cut(s) | 806 |
| BsaJI | CCNNGG | 7 cut(s) | 172, 406, 444, 499, 731, 1477, 1525 |
| BsaWI | WCCGGW | 2 cut(s) | 1039, 1168 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 1206, 1236, 1281, 1311, 1521, 1551 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1185, 1526 |
| Bse1I | ACTGG | 1 cut(s) | 765 |
| Bse3DI | GCAATG | 2 cut(s) | 336, 1449 |
| BseAI | TCCGGA | 1 cut(s) | 1039 |
| BseBI | CCWGG | 4 cut(s) | 733, 741, 1028, 1479 |
| BseDI | CCNNGG | 7 cut(s) | 172, 406, 444, 499, 731, 1477, 1525 |
| BseGI | GGATG | 1 cut(s) | 1036 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1185, 1526 |
| BseMI | GCAATG | 2 cut(s) | 336, 1449 |
| BseMII | CTCAG | 6 cut(s) | 339, 341, 537, 739, 762, 1338 |
| BseNI | ACTGG | 1 cut(s) | 765 |
| BseXI | GCAGC | 2 cut(s) | 663, 666 |
| BseYI | CCCAGC | 1 cut(s) | 1210 |
| BsgI | GTGCAG | 1 cut(s) | 1529 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1362 |
| BshFI | GGCC | 6 cut(s) | 153, 223, 485, 730, 848, 1519 |
| BsiEI | CGRYCG | 1 cut(s) | 1362 |
| BsiSI | CCGG | 3 cut(s) | 1040, 1169, 1229 |
| BslFI | GGGAC | 3 cut(s) | 388, 1235, 1398 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1185, 1526 |
| BsmAI | GTCTC | 4 cut(s) | 12, 40, 542, 1155 |
| BsmFI | GGGAC | 3 cut(s) | 388, 1235, 1398 |
| BsnI | GGCC | 6 cut(s) | 153, 223, 485, 730, 848, 1519 |
| Bsp119I | TTCGAA | 1 cut(s) | 391 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1221 |
| Bsp13I | TCCGGA | 1 cut(s) | 1039 |
| Bsp143I | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| Bsp19I | CCATGG | 3 cut(s) | 172, 444, 499 |
| BspACI | CCGC | 3 cut(s) | 341, 642, 782 |
| BspANI | GGCC | 6 cut(s) | 153, 223, 485, 730, 848, 1519 |
| BspCNI | CTCAG | 6 cut(s) | 340, 340, 538, 738, 763, 1337 |
| BspEI | TCCGGA | 1 cut(s) | 1039 |
| BspHI | TCATGA | 4 cut(s) | 192, 574, 910, 1270 |
| BspLI | GGNNCC | 4 cut(s) | 108, 345, 525, 1386 |
| BspPI | GGATC | 2 cut(s) | 92, 1268 |
| BspT104I | TTCGAA | 1 cut(s) | 391 |
| BsrBI | CCGCTC | 1 cut(s) | 782 |
| BsrDI | GCAATG | 2 cut(s) | 336, 1449 |
| BsrI | ACTGG | 1 cut(s) | 765 |
| BssECI | CCNNGG | 7 cut(s) | 172, 406, 444, 499, 731, 1477, 1525 |
| BssMI | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| BssT1I | CCWWGG | 4 cut(s) | 172, 406, 444, 499 |
| Bst2UI | CCWGG | 4 cut(s) | 733, 741, 1028, 1479 |
| Bst4CI | ACNGT | 7 cut(s) | 34, 256, 1018, 1360, 1513, 1526, 1554 |
| Bst6I | CTCTTC | 1 cut(s) | 665 |
| BstBAI | YACGTR | 1 cut(s) | 806 |
| BstBI | TTCGAA | 1 cut(s) | 391 |
| BstC8I | GCNNGC | 3 cut(s) | 221, 483, 1008 |
| BstDEI | CTNAG | 9 cut(s) | 70, 327, 348, 546, 661, 725, 771, 1154, 1324 |
| BstDSI | CCRYGG | 4 cut(s) | 172, 444, 499, 1525 |
| BstF5I | GGATG | 1 cut(s) | 1036 |
| BstH2I | RGCGCY | 1 cut(s) | 1459 |
| BstHHI | GCGC | 1 cut(s) | 1458 |
| BstKTI | GATC | 5 cut(s) | 87, 591, 777, 1140, 1276 |
| BstMAI | GTCTC | 4 cut(s) | 12, 40, 542, 1155 |
| BstMBI | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| BstMCI | CGRYCG | 1 cut(s) | 1362 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 491, 1007, 1216 |
| BstNI | CCWGG | 4 cut(s) | 733, 741, 1028, 1479 |
| BstNSI | RCATGY | 2 cut(s) | 235, 1398 |
| BstSCI | CCNGG | 4 cut(s) | 731, 739, 1026, 1477 |
| BstSFI | CTRYAG | 3 cut(s) | 252, 1451, 1459 |
| BstV1I | GCAGC | 2 cut(s) | 663, 666 |
| BstV2I | GAAGAC | 1 cut(s) | 1299 |
| BstX2I | RGATCY | 1 cut(s) | 84 |
| BstYI | RGATCY | 1 cut(s) | 84 |
| BsuRI | GGCC | 6 cut(s) | 153, 223, 485, 730, 848, 1519 |
| BtgI | CCRYGG | 4 cut(s) | 172, 444, 499, 1525 |
| BtrI | CACGTC | 2 cut(s) | 1249, 1297 |
| BtsCI | GGATG | 1 cut(s) | 1036 |
| BtsIMutI | CAGTG | 4 cut(s) | 30, 543, 772, 1550 |
| Cac8I | GCNNGC | 3 cut(s) | 221, 483, 1008 |
| CciI | TCATGA | 4 cut(s) | 192, 574, 910, 1270 |
| CfoI | GCGC | 1 cut(s) | 1458 |
| Cfr13I | GGNCC | 5 cut(s) | 152, 279, 639, 729, 1385 |
| Csp6I | GTAC | 2 cut(s) | 318, 893 |
| CviQI | GTAC | 2 cut(s) | 318, 893 |
| DdeI | CTNAG | 9 cut(s) | 70, 327, 348, 546, 661, 725, 771, 1154, 1324 |
| DpnI | GATC | 5 cut(s) | 86, 590, 776, 1139, 1275 |
| DpnII | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| DraIII | CACNNNGTG | 1 cut(s) | 1526 |
| EaeI | YGGCCR | 2 cut(s) | 483, 1517 |
| Eam1104I | CTCTTC | 1 cut(s) | 665 |
| EarI | CTCTTC | 1 cut(s) | 665 |
| Eco130I | CCWWGG | 4 cut(s) | 172, 406, 444, 499 |
| Eco24I | GRGCYC | 1 cut(s) | 1221 |
| Eco47I | GGWCC | 3 cut(s) | 279, 639, 1385 |
| Eco47III | AGCGCT | 1 cut(s) | 1457 |
| Eco57I | CTGAAG | 2 cut(s) | 606, 920 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1385 |
| EcoRII | CCWGG | 4 cut(s) | 731, 739, 1026, 1477 |
| EcoT14I | CCWWGG | 4 cut(s) | 172, 406, 444, 499 |
| EcoT38I | GRGCYC | 1 cut(s) | 1221 |
| ErhI | CCWWGG | 4 cut(s) | 172, 406, 444, 499 |
| FaqI | GGGAC | 3 cut(s) | 388, 1235, 1398 |
| FbaI | TGATCA | 1 cut(s) | 1137 |
| Fnu4HI | GCNGC | 2 cut(s) | 652, 655 |
| FokI | GGATG | 1 cut(s) | 1023 |
| FriOI | GRGCYC | 1 cut(s) | 1221 |
| Fsp4HI | GCNGC | 2 cut(s) | 652, 655 |
| FspBI | CTAG | 1 cut(s) | 540 |
| GlaI | GCGC | 1 cut(s) | 1457 |
| GluI | GCNGC | 2 cut(s) | 652, 655 |
| GsaI | CCCAGC | 1 cut(s) | 1214 |
| HaeII | RGCGCY | 1 cut(s) | 1459 |
| HaeIII | GGCC | 6 cut(s) | 153, 223, 485, 730, 848, 1519 |
| HapII | CCGG | 3 cut(s) | 1040, 1169, 1229 |
| HhaI | GCGC | 1 cut(s) | 1458 |
| Hin6I | GCGC | 1 cut(s) | 1456 |
| HinP1I | GCGC | 1 cut(s) | 1456 |
| HincII | GTYRAC | 3 cut(s) | 618, 699, 1088 |
| HindII | GTYRAC | 3 cut(s) | 618, 699, 1088 |
| HindIII | AAGCTT | 1 cut(s) | 788 |
| HinfI | GANTC | 5 cut(s) | 20, 1148, 1255, 1320, 1447 |
| HpaII | CCGG | 3 cut(s) | 1040, 1169, 1229 |
| HphI | GGTGA | 4 cut(s) | 263, 467, 1459, 1485 |
| Hpy166II | GTNNAC | 6 cut(s) | 30, 618, 699, 1088, 1300, 1489 |
| Hpy188I | TCNGA | 4 cut(s) | 625, 779, 900, 1327 |
| Hpy188III | TCNNGA | 9 cut(s) | 82, 193, 527, 575, 911, 1040, 1103, 1252, 1271 |
| Hpy8I | GTNNAC | 6 cut(s) | 30, 618, 699, 1088, 1300, 1489 |
| HpyAV | CCTTC | 8 cut(s) | 446, 505, 572, 659, 790, 895, 1322, 1375 |
| HpyCH4III | ACNGT | 7 cut(s) | 34, 256, 1018, 1360, 1513, 1526, 1554 |
| HpyCH4IV | ACGT | 4 cut(s) | 805, 968, 1248, 1296 |
| HpyCH4V | TGCA | 5 cut(s) | 139, 963, 1246, 1262, 1546 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 491, 1007, 1216 |
| HpyF3I | CTNAG | 9 cut(s) | 70, 327, 348, 546, 661, 725, 771, 1154, 1324 |
| HpySE526I | ACGT | 4 cut(s) | 805, 968, 1248, 1296 |
| HspAI | GCGC | 1 cut(s) | 1456 |
| Kpn2I | TCCGGA | 1 cut(s) | 1039 |
| Ksp22I | TGATCA | 1 cut(s) | 1137 |
| Kzo9I | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| Lsp1109I | GCAGC | 2 cut(s) | 663, 666 |
| LweI | GCATC | 1 cut(s) | 753 |
| MaeI | CTAG | 1 cut(s) | 540 |
| MaeII | ACGT | 4 cut(s) | 805, 968, 1248, 1296 |
| MaeIII | GTNAC | 3 cut(s) | 234, 806, 1352 |
| MalI | GATC | 5 cut(s) | 86, 590, 776, 1139, 1275 |
| MbiI | CCGCTC | 1 cut(s) | 782 |
| MboI | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| MboII | GAAGA | 6 cut(s) | 296, 307, 430, 614, 682, 1304 |
| MflI | RGATCY | 1 cut(s) | 84 |
| MhlI | GDGCHC | 1 cut(s) | 1221 |
| MlsI | TGGCCA | 1 cut(s) | 485 |
| MluCI | AATT | 9 cut(s) | 63, 125, 298, 605, 675, 1076, 1241, 1280, 1497 |
| MluNI | TGGCCA | 1 cut(s) | 485 |
| MlyI | GAGTC | 2 cut(s) | 14, 1157 |
| MmeI | TCCRAC | 1 cut(s) | 1121 |
| MnlI | CCTC | 9 cut(s) | 273, 336, 586, 712, 924, 1045, 1176, 1208, 1398 |
| Mox20I | TGGCCA | 1 cut(s) | 485 |
| MroI | TCCGGA | 1 cut(s) | 1039 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1304 |
| MscI | TGGCCA | 1 cut(s) | 485 |
| MseI | TTAA | 4 cut(s) | 473, 512, 828, 1206 |
| MslI | CAYNNNNRTG | 2 cut(s) | 197, 749 |
| Msp20I | TGGCCA | 1 cut(s) | 485 |
| MspI | CCGG | 3 cut(s) | 1040, 1169, 1229 |
| MspR9I | CCNGG | 4 cut(s) | 733, 741, 1028, 1479 |
| MvaI | CCWGG | 4 cut(s) | 733, 741, 1028, 1479 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 491, 1007, 1216 |
| NcoI | CCATGG | 3 cut(s) | 172, 444, 499 |
| NdeII | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| NlaIV | GGNNCC | 4 cut(s) | 108, 345, 525, 1386 |
| NmeAIII | GCCGAG | 1 cut(s) | 844 |
| NmuCI | GTSAC | 2 cut(s) | 234, 806 |
| NspI | RCATGY | 2 cut(s) | 235, 1398 |
| NspV | TTCGAA | 1 cut(s) | 391 |
| PagI | TCATGA | 4 cut(s) | 192, 574, 910, 1270 |
| PciI | ACATGT | 2 cut(s) | 231, 1394 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1522 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1304 |
| PfeI | GAWTC | 3 cut(s) | 1255, 1320, 1447 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1526 |
| PkrI | GCNGC | 2 cut(s) | 653, 656 |
| PleI | GAGTC | 2 cut(s) | 14, 1156 |
| PpsI | GAGTC | 2 cut(s) | 14, 1156 |
| Ppu21I | YACGTR | 1 cut(s) | 806 |
| PpuMI | RGGWCCY | 1 cut(s) | 1385 |
| PscI | ACATGT | 2 cut(s) | 231, 1394 |
| Psp1406I | AACGTT | 1 cut(s) | 968 |
| Psp5II | RGGWCCY | 1 cut(s) | 1385 |
| Psp6I | CCWGG | 4 cut(s) | 731, 739, 1026, 1477 |
| PspFI | CCCAGC | 1 cut(s) | 1210 |
| PspGI | CCWGG | 4 cut(s) | 731, 739, 1026, 1477 |
| PspN4I | GGNNCC | 4 cut(s) | 108, 345, 525, 1386 |
| PspPI | GGNCC | 5 cut(s) | 152, 279, 639, 729, 1385 |
| PspPPI | RGGWCCY | 1 cut(s) | 1385 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 90, 122 |
| PsuI | RGATCY | 1 cut(s) | 84 |
| RsaI | GTAC | 2 cut(s) | 319, 894 |
| RsaNI | GTAC | 2 cut(s) | 318, 893 |
| RseI | CAYNNNNRTG | 2 cut(s) | 197, 749 |
| SaqAI | TTAA | 4 cut(s) | 473, 512, 828, 1206 |
| SatI | GCNGC | 2 cut(s) | 652, 655 |
| Sau3AI | GATC | 5 cut(s) | 84, 588, 774, 1137, 1273 |
| Sau96I | GGNCC | 5 cut(s) | 152, 279, 639, 729, 1385 |
| ScaI | AGTACT | 1 cut(s) | 894 |
| SchI | GAGTC | 2 cut(s) | 14, 1157 |
| ScrFI | CCNGG | 4 cut(s) | 733, 741, 1028, 1479 |
| SduI | GDGCHC | 1 cut(s) | 1221 |
| SfaNI | GCATC | 1 cut(s) | 753 |
| SfcI | CTRYAG | 3 cut(s) | 252, 1451, 1459 |
| SfuI | TTCGAA | 1 cut(s) | 391 |
| SinI | GGWCC | 3 cut(s) | 279, 639, 1385 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 197, 749 |
| SmlI | CTYRAG | 2 cut(s) | 37, 791 |
| SmoI | CTYRAG | 2 cut(s) | 37, 791 |
| Sse9I | AATT | 9 cut(s) | 63, 125, 298, 605, 675, 1076, 1241, 1280, 1497 |
| SsiI | CCGC | 3 cut(s) | 341, 642, 782 |
| SspMI | CTAG | 1 cut(s) | 540 |
| StyD4I | CCNGG | 4 cut(s) | 731, 739, 1026, 1477 |
| StyI | CCWWGG | 4 cut(s) | 172, 406, 444, 499 |
| TaaI | ACNGT | 7 cut(s) | 34, 256, 1018, 1360, 1513, 1526, 1554 |
| TaiI | ACGT | 4 cut(s) | 808, 971, 1251, 1299 |
| TaqI | TCGA | 5 cut(s) | 23, 391, 591, 942, 1390 |
| TasI | AATT | 9 cut(s) | 63, 125, 298, 605, 675, 1076, 1241, 1280, 1497 |
| TatI | WGTACW | 1 cut(s) | 892 |
| TfiI | GAWTC | 3 cut(s) | 1255, 1320, 1447 |
| Tru1I | TTAA | 4 cut(s) | 473, 512, 828, 1206 |
| Tru9I | TTAA | 4 cut(s) | 473, 512, 828, 1206 |
| TscAI | CASTG | 4 cut(s) | 37, 550, 772, 1557 |
| TseFI | GTSAC | 2 cut(s) | 234, 806 |
| TseI | GCWGC | 2 cut(s) | 651, 654 |
| Tsp45I | GTSAC | 2 cut(s) | 234, 806 |
| TspDTI | ATGAA | 7 cut(s) | 118, 215, 591, 866, 936, 1256, 1421 |
| TspRI | CASTG | 4 cut(s) | 37, 550, 772, 1557 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1526 |
| VpaK11BI | GGWCC | 3 cut(s) | 279, 639, 1385 |
| XapI | RAATTY | 5 cut(s) | 125, 298, 605, 675, 1280 |
| XceI | RCATGY | 2 cut(s) | 235, 1398 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 497 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1304 |
| XspI | CTAG | 1 cut(s) | 540 |
| ZrmI | AGTACT | 1 cut(s) | 894 |