cytochrome P450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 381 |
| AcsI | RAATTY | 1 cut(s) | 578 |
| AcuI | CTGAAG | 2 cut(s) | 263, 579 |
| AfaI | GTAC | 1 cut(s) | 292 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 192, 615 |
| AgsI | TTSAA | 3 cut(s) | 9, 169, 233 |
| AjnI | CCWGG | 1 cut(s) | 622 |
| AleI | CACNNNNGTG | 1 cut(s) | 73 |
| AluBI | AGCT | 6 cut(s) | 12, 41, 483, 670, 750, 782 |
| AluI | AGCT | 6 cut(s) | 12, 41, 483, 670, 750, 782 |
| Alw26I | GTCTC | 1 cut(s) | 860 |
| AlwI | GGATC | 1 cut(s) | 381 |
| Ama87I | CYCGRG | 1 cut(s) | 884 |
| AoxI | GGCC | 3 cut(s) | 124, 194, 409 |
| ApeKI | GCWGC | 2 cut(s) | 419, 529 |
| ApoI | RAATTY | 1 cut(s) | 578 |
| AspS9I | GGNCC | 4 cut(s) | 125, 195, 891, 946 |
| AsuHPI | GGTGA | 1 cut(s) | 319 |
| AvaI | CYCGRG | 1 cut(s) | 884 |
| AvaII | GGWCC | 2 cut(s) | 891, 946 |
| BauI | CACGAG | 1 cut(s) | 548 |
| BbsI | GAAGAC | 1 cut(s) | 750 |
| BbvCI | CCTCAGC | 1 cut(s) | 423 |
| BbvI | GCAGC | 2 cut(s) | 406, 516 |
| BccI | CCATC | 3 cut(s) | 97, 194, 977 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 356, 390 |
| BciT130I | CCWGG | 1 cut(s) | 624 |
| BclI | TGATCA | 1 cut(s) | 51 |
| BcoDI | GTCTC | 1 cut(s) | 860 |
| BfaI | CTAG | 1 cut(s) | 398 |
| BglII | AGATCT | 1 cut(s) | 57 |
| BisI | GCNGC | 2 cut(s) | 420, 530 |
| BlsI | GCNGC | 2 cut(s) | 421, 531 |
| Bme1390I | CCNGG | 1 cut(s) | 624 |
| Bme18I | GGWCC | 2 cut(s) | 891, 946 |
| BmeT110I | CYCGRG | 1 cut(s) | 884 |
| BmgT120I | GGNCC | 4 cut(s) | 125, 195, 891, 946 |
| BmrFI | CCNGG | 1 cut(s) | 624 |
| BmsI | GCATC | 2 cut(s) | 516, 636 |
| BpiI | GAAGAC | 1 cut(s) | 750 |
| BpmI | CTGGAG | 1 cut(s) | 772 |
| Bpu10I | CCTNAGC | 1 cut(s) | 423 |
| BpuEI | CTTGAG | 1 cut(s) | 692 |
| BsaBI | GATNNNNATC | 2 cut(s) | 50, 56 |
| BsaI | GGTCTC | 1 cut(s) | 860 |
| BsaJI | CCNNGG | 3 cut(s) | 145, 379, 412 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 422, 452 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 192, 615 |
| Bse1I | ACTGG | 1 cut(s) | 513 |
| Bse3DI | GCAATG | 2 cut(s) | 735, 987 |
| Bse8I | GATNNNNATC | 2 cut(s) | 50, 56 |
| BseBI | CCWGG | 1 cut(s) | 624 |
| BseDI | CCNNGG | 3 cut(s) | 145, 379, 412 |
| BseGI | GGATG | 1 cut(s) | 826 |
| BseJI | GATNNNNATC | 2 cut(s) | 50, 56 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 192, 615 |
| BseMI | GCAATG | 2 cut(s) | 735, 987 |
| BseMII | CTCAG | 4 cut(s) | 312, 314, 414, 510 |
| BseNI | ACTGG | 1 cut(s) | 513 |
| BseXI | GCAGC | 2 cut(s) | 406, 516 |
| BshFI | GGCC | 3 cut(s) | 126, 196, 411 |
| BsiHKCI | CYCGRG | 1 cut(s) | 884 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 192, 615 |
| BsmAI | GTCTC | 1 cut(s) | 860 |
| BsnI | GGCC | 3 cut(s) | 126, 196, 411 |
| Bso31I | GGTCTC | 1 cut(s) | 860 |
| BsoBI | CYCGRG | 1 cut(s) | 884 |
| Bsp143I | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| Bsp19I | CCATGG | 1 cut(s) | 145 |
| BspANI | GGCC | 3 cut(s) | 126, 196, 411 |
| BspCNI | CTCAG | 4 cut(s) | 313, 313, 415, 511 |
| BspPI | GGATC | 1 cut(s) | 381 |
| BspTNI | GGTCTC | 1 cut(s) | 860 |
| BsrDI | GCAATG | 2 cut(s) | 735, 987 |
| BsrI | ACTGG | 1 cut(s) | 513 |
| BssECI | CCNNGG | 3 cut(s) | 145, 379, 412 |
| BssMI | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| BssSI | CACGAG | 1 cut(s) | 548 |
| BssT1I | CCWWGG | 3 cut(s) | 145, 379, 412 |
| Bst2BI | CACGAG | 1 cut(s) | 548 |
| Bst2UI | CCWGG | 1 cut(s) | 624 |
| Bst4CI | ACNGT | 3 cut(s) | 692, 895, 1006 |
| BstC8I | GCNNGC | 2 cut(s) | 92, 194 |
| BstDEI | CTNAG | 4 cut(s) | 300, 321, 423, 519 |
| BstDSI | CCRYGG | 1 cut(s) | 145 |
| BstF5I | GGATG | 1 cut(s) | 826 |
| BstKTI | GATC | 5 cut(s) | 48, 54, 60, 312, 376 |
| BstMAI | GTCTC | 1 cut(s) | 860 |
| BstMBI | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 464 |
| BstNI | CCWGG | 1 cut(s) | 624 |
| BstSCI | CCNGG | 1 cut(s) | 622 |
| BstV1I | GCAGC | 2 cut(s) | 406, 516 |
| BstV2I | GAAGAC | 1 cut(s) | 750 |
| BstX2I | RGATCY | 1 cut(s) | 57 |
| BstYI | RGATCY | 1 cut(s) | 57 |
| BsuRI | GGCC | 3 cut(s) | 126, 196, 411 |
| BtgI | CCRYGG | 1 cut(s) | 145 |
| BtsCI | GGATG | 1 cut(s) | 826 |
| BtsIMutI | CAGTG | 3 cut(s) | 287, 520, 822 |
| Cac8I | GCNNGC | 2 cut(s) | 92, 194 |
| Cfr13I | GGNCC | 4 cut(s) | 125, 195, 891, 946 |
| Csp6I | GTAC | 1 cut(s) | 291 |
| CviAII | CATG | 6 cut(s) | 146, 172, 212, 249, 287, 473 |
| CviQI | GTAC | 1 cut(s) | 291 |
| DdeI | CTNAG | 4 cut(s) | 300, 321, 423, 519 |
| DpnI | GATC | 5 cut(s) | 47, 53, 59, 311, 375 |
| DpnII | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| DraI | TTTAAA | 2 cut(s) | 640, 777 |
| Eco130I | CCWWGG | 3 cut(s) | 145, 379, 412 |
| Eco31I | GGTCTC | 1 cut(s) | 860 |
| Eco47I | GGWCC | 2 cut(s) | 891, 946 |
| Eco57I | CTGAAG | 2 cut(s) | 263, 579 |
| Eco88I | CYCGRG | 1 cut(s) | 884 |
| EcoO109I | RGGNCCY | 2 cut(s) | 125, 946 |
| EcoRII | CCWGG | 1 cut(s) | 622 |
| EcoT14I | CCWWGG | 3 cut(s) | 145, 379, 412 |
| ErhI | CCWWGG | 3 cut(s) | 145, 379, 412 |
| FaeI | CATG | 6 cut(s) | 149, 175, 215, 252, 290, 476 |
| FatI | CATG | 6 cut(s) | 145, 171, 211, 248, 286, 472 |
| FbaI | TGATCA | 1 cut(s) | 51 |
| Fnu4HI | GCNGC | 2 cut(s) | 420, 530 |
| FokI | GGATG | 1 cut(s) | 833 |
| Fsp4HI | GCNGC | 2 cut(s) | 420, 530 |
| FspBI | CTAG | 1 cut(s) | 398 |
| GluI | GCNGC | 2 cut(s) | 420, 530 |
| GsuI | CTGGAG | 1 cut(s) | 772 |
| HaeIII | GGCC | 3 cut(s) | 126, 196, 411 |
| Hin1II | CATG | 6 cut(s) | 149, 175, 215, 252, 290, 476 |
| HincII | GTYRAC | 2 cut(s) | 591, 709 |
| HindII | GTYRAC | 2 cut(s) | 591, 709 |
| HindIII | AAGCTT | 2 cut(s) | 10, 668 |
| HinfI | GANTC | 7 cut(s) | 79, 117, 138, 496, 612, 659, 791 |
| HphI | GGTGA | 1 cut(s) | 319 |
| Hpy166II | GTNNAC | 5 cut(s) | 78, 591, 709, 719, 891 |
| Hpy188I | TCNGA | 3 cut(s) | 45, 598, 1010 |
| Hpy8I | GTNNAC | 5 cut(s) | 78, 591, 709, 719, 891 |
| HpyAV | CCTTC | 3 cut(s) | 262, 718, 934 |
| HpyCH4III | ACNGT | 3 cut(s) | 692, 895, 1006 |
| HpyCH4IV | ACGT | 1 cut(s) | 715 |
| HpyCH4V | TGCA | 6 cut(s) | 94, 330, 367, 532, 840, 952 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 464 |
| HpyF3I | CTNAG | 4 cut(s) | 300, 321, 423, 519 |
| HpySE526I | ACGT | 1 cut(s) | 715 |
| Hsp92II | CATG | 6 cut(s) | 149, 175, 215, 252, 290, 476 |
| Ksp22I | TGATCA | 1 cut(s) | 51 |
| Kzo9I | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| LmnI | GCTCC | 1 cut(s) | 430 |
| Lsp1109I | GCAGC | 2 cut(s) | 406, 516 |
| LweI | GCATC | 2 cut(s) | 516, 636 |
| MaeI | CTAG | 1 cut(s) | 398 |
| MaeII | ACGT | 1 cut(s) | 715 |
| MaeIII | GTNAC | 2 cut(s) | 131, 755 |
| MalI | GATC | 5 cut(s) | 47, 53, 59, 311, 375 |
| MboI | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| MboII | GAAGA | 5 cut(s) | 269, 403, 535, 755, 893 |
| MflI | RGATCY | 1 cut(s) | 57 |
| MluCI | AATT | 3 cut(s) | 443, 578, 953 |
| MlyI | GAGTC | 3 cut(s) | 73, 132, 621 |
| MmeI | TCCRAC | 1 cut(s) | 107 |
| MnlI | CCTC | 8 cut(s) | 234, 309, 418, 544, 559, 801, 922, 937 |
| MseI | TTAA | 4 cut(s) | 206, 485, 639, 776 |
| MslI | CAYNNNNRTG | 1 cut(s) | 73 |
| MspA1I | CMGCKG | 1 cut(s) | 750 |
| MspR9I | CCNGG | 1 cut(s) | 624 |
| MvaI | CCWGG | 1 cut(s) | 624 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 464 |
| NcoI | CCATGG | 1 cut(s) | 145 |
| NdeII | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| NlaIII | CATG | 6 cut(s) | 149, 175, 215, 252, 290, 476 |
| NmeAIII | GCCGAG | 1 cut(s) | 88 |
| NmuCI | GTSAC | 1 cut(s) | 755 |
| OliI | CACNNNNGTG | 1 cut(s) | 73 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 891 |
| PfeI | GAWTC | 4 cut(s) | 117, 496, 659, 791 |
| PkrI | GCNGC | 2 cut(s) | 421, 531 |
| PleI | GAGTC | 3 cut(s) | 73, 132, 620 |
| PpsI | GAGTC | 3 cut(s) | 73, 132, 620 |
| PpuMI | RGGWCCY | 1 cut(s) | 946 |
| Psp5II | RGGWCCY | 1 cut(s) | 946 |
| Psp6I | CCWGG | 1 cut(s) | 622 |
| PspGI | CCWGG | 1 cut(s) | 622 |
| PspPI | GGNCC | 4 cut(s) | 125, 195, 891, 946 |
| PspPPI | RGGWCCY | 1 cut(s) | 946 |
| PsuI | RGATCY | 1 cut(s) | 57 |
| PvuII | CAGCTG | 1 cut(s) | 750 |
| RsaI | GTAC | 1 cut(s) | 292 |
| RsaNI | GTAC | 1 cut(s) | 291 |
| RseI | CAYNNNNRTG | 1 cut(s) | 73 |
| SaqAI | TTAA | 4 cut(s) | 206, 485, 639, 776 |
| SatI | GCNGC | 2 cut(s) | 420, 530 |
| Sau3AI | GATC | 5 cut(s) | 45, 51, 57, 309, 373 |
| Sau96I | GGNCC | 4 cut(s) | 125, 195, 891, 946 |
| SchI | GAGTC | 3 cut(s) | 73, 132, 621 |
| ScrFI | CCNGG | 1 cut(s) | 624 |
| SfaNI | GCATC | 2 cut(s) | 516, 636 |
| SinI | GGWCC | 2 cut(s) | 891, 946 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 73 |
| SmlI | CTYRAG | 1 cut(s) | 671 |
| SmoI | CTYRAG | 1 cut(s) | 671 |
| Sse9I | AATT | 3 cut(s) | 443, 578, 953 |
| SspMI | CTAG | 1 cut(s) | 398 |
| StyD4I | CCNGG | 1 cut(s) | 622 |
| StyI | CCWWGG | 3 cut(s) | 145, 379, 412 |
| TaaI | ACNGT | 3 cut(s) | 692, 895, 1006 |
| TaiI | ACGT | 1 cut(s) | 718 |
| TaqI | TCGA | 1 cut(s) | 657 |
| TasI | AATT | 3 cut(s) | 443, 578, 953 |
| TfiI | GAWTC | 4 cut(s) | 117, 496, 659, 791 |
| Tru1I | TTAA | 4 cut(s) | 206, 485, 639, 776 |
| Tru9I | TTAA | 4 cut(s) | 206, 485, 639, 776 |
| TscAI | CASTG | 3 cut(s) | 287, 520, 822 |
| TseFI | GTSAC | 1 cut(s) | 755 |
| TseI | GCWGC | 2 cut(s) | 419, 529 |
| Tsp45I | GTSAC | 1 cut(s) | 755 |
| TspDTI | ATGAA | 5 cut(s) | 188, 195, 461, 666, 714 |
| TspGWI | ACGGA | 1 cut(s) | 932 |
| TspRI | CASTG | 3 cut(s) | 287, 520, 822 |
| VpaK11BI | GGWCC | 2 cut(s) | 891, 946 |
| XapI | RAATTY | 1 cut(s) | 578 |
| XspI | CTAG | 1 cut(s) | 398 |