Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1526 |
| AclWI | GGATC | 3 cut(s) | 70, 143, 1268 |
| AcoI | YGGCCR | 2 cut(s) | 477, 1517 |
| AcsI | RAATTY | 1 cut(s) | 795 |
| AcuI | CTGAAG | 1 cut(s) | 1443 |
| AdeI | CACNNNGTG | 1 cut(s) | 406 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1526 |
| AjnI | CCWGG | 3 cut(s) | 733, 1026, 1477 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 401, 433 |
| Alw26I | GTCTC | 2 cut(s) | 536, 1255 |
| AlwI | GGATC | 3 cut(s) | 70, 143, 1268 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 338, 358 |
| AoxI | GGCC | 5 cut(s) | 142, 212, 427, 477, 1517 |
| ApeKI | GCWGC | 3 cut(s) | 645, 648, 1543 |
| ApoI | RAATTY | 1 cut(s) | 795 |
| AspS9I | GGNCC | 4 cut(s) | 143, 213, 270, 1385 |
| AsuHPI | GGTGA | 2 cut(s) | 1259, 1459 |
| AvaII | GGWCC | 2 cut(s) | 270, 1385 |
| BalI | TGGCCA | 1 cut(s) | 479 |
| BanII | GRGCYC | 1 cut(s) | 396 |
| BauI | CACGAG | 1 cut(s) | 265 |
| BbvI | GCAGC | 3 cut(s) | 657, 660, 1530 |
| BccI | CCATC | 3 cut(s) | 115, 419, 1100 |
| BciT130I | CCWGG | 3 cut(s) | 735, 1028, 1479 |
| BclI | TGATCA | 1 cut(s) | 1137 |
| BcoDI | GTCTC | 2 cut(s) | 536, 1255 |
| BfaI | CTAG | 4 cut(s) | 534, 551, 1211, 1230 |
| BfmI | CTRYAG | 1 cut(s) | 243 |
| BisI | GCNGC | 3 cut(s) | 646, 649, 1544 |
| BlsI | GCNGC | 3 cut(s) | 647, 650, 1545 |
| Bme1390I | CCNGG | 3 cut(s) | 735, 1028, 1479 |
| Bme18I | GGWCC | 2 cut(s) | 270, 1385 |
| BmgT120I | GGNCC | 4 cut(s) | 143, 213, 270, 1385 |
| BmiI | GGNNCC | 3 cut(s) | 215, 732, 1386 |
| BmrFI | CCNGG | 3 cut(s) | 735, 1028, 1479 |
| BmsI | GCATC | 1 cut(s) | 537 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1307, 1339 |
| BpmI | CTGGAG | 1 cut(s) | 870 |
| BpuEI | CTTGAG | 1 cut(s) | 806 |
| BsaJI | CCNNGG | 2 cut(s) | 493, 1477 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1521, 1551 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1526 |
| Bse3DI | GCAATG | 2 cut(s) | 849, 1001 |
| BseBI | CCWGG | 3 cut(s) | 735, 1028, 1479 |
| BseDI | CCNNGG | 2 cut(s) | 493, 1477 |
| BseGI | GGATG | 1 cut(s) | 949 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1526 |
| BseMI | GCAATG | 2 cut(s) | 849, 1001 |
| BseMII | CTCAG | 3 cut(s) | 330, 332, 1338 |
| BseXI | GCAGC | 3 cut(s) | 657, 660, 1530 |
| BsgI | GTGCAG | 1 cut(s) | 1529 |
| BshFI | GGCC | 5 cut(s) | 144, 214, 429, 479, 1519 |
| BslFI | GGGAC | 2 cut(s) | 1302, 1398 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1526 |
| BsmAI | GTCTC | 2 cut(s) | 536, 1255 |
| BsmBI | CGTCTC | 1 cut(s) | 536 |
| BsmFI | GGGAC | 2 cut(s) | 1302, 1398 |
| BsmI | GAATGC | 1 cut(s) | 1463 |
| BsnI | GGCC | 5 cut(s) | 144, 214, 429, 479, 1519 |
| Bsp1286I | GDGCHC | 1 cut(s) | 396 |
| Bsp143I | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| Bsp19I | CCATGG | 1 cut(s) | 493 |
| BspANI | GGCC | 5 cut(s) | 144, 214, 429, 479, 1519 |
| BspCNI | CTCAG | 3 cut(s) | 331, 331, 1337 |
| BspHI | TCATGA | 1 cut(s) | 904 |
| BspLI | GGNNCC | 3 cut(s) | 215, 732, 1386 |
| BspPI | GGATC | 3 cut(s) | 70, 143, 1268 |
| BsrDI | GCAATG | 2 cut(s) | 849, 1001 |
| BssECI | CCNNGG | 2 cut(s) | 493, 1477 |
| BssMI | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| BssSI | CACGAG | 1 cut(s) | 265 |
| BssT1I | CCWWGG | 1 cut(s) | 493 |
| Bst2BI | CACGAG | 1 cut(s) | 265 |
| Bst2UI | CCWGG | 3 cut(s) | 735, 1028, 1479 |
| Bst4CI | ACNGT | 3 cut(s) | 565, 1018, 1513 |
| Bst6I | CTCTTC | 1 cut(s) | 591 |
| BstC8I | GCNNGC | 2 cut(s) | 212, 477 |
| BstDEI | CTNAG | 5 cut(s) | 61, 318, 339, 1154, 1324 |
| BstDSI | CCRYGG | 1 cut(s) | 493 |
| BstF5I | GGATG | 1 cut(s) | 949 |
| BstKTI | GATC | 4 cut(s) | 78, 138, 1140, 1276 |
| BstMAI | GTCTC | 2 cut(s) | 536, 1255 |
| BstMBI | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 485, 1433 |
| BstNI | CCWGG | 3 cut(s) | 735, 1028, 1479 |
| BstSCI | CCNGG | 3 cut(s) | 733, 1026, 1477 |
| BstSFI | CTRYAG | 1 cut(s) | 243 |
| BstV1I | GCAGC | 3 cut(s) | 657, 660, 1530 |
| BstX2I | RGATCY | 1 cut(s) | 75 |
| BstYI | RGATCY | 1 cut(s) | 75 |
| BsuRI | GGCC | 5 cut(s) | 144, 214, 429, 479, 1519 |
| BtgI | CCRYGG | 1 cut(s) | 493 |
| BtgZI | GCGATG | 2 cut(s) | 438, 777 |
| BtsCI | GGATG | 1 cut(s) | 949 |
| Cac8I | GCNNGC | 2 cut(s) | 212, 477 |
| CaiI | CAGNNNCTG | 2 cut(s) | 338, 358 |
| CciI | TCATGA | 1 cut(s) | 904 |
| Cfr13I | GGNCC | 4 cut(s) | 143, 213, 270, 1385 |
| DdeI | CTNAG | 5 cut(s) | 61, 318, 339, 1154, 1324 |
| DpnI | GATC | 4 cut(s) | 77, 137, 1139, 1275 |
| DpnII | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| DraI | TTTAAA | 1 cut(s) | 507 |
| DraIII | CACNNNGTG | 1 cut(s) | 406 |
| EaeI | YGGCCR | 2 cut(s) | 477, 1517 |
| Eam1104I | CTCTTC | 1 cut(s) | 591 |
| EarI | CTCTTC | 1 cut(s) | 591 |
| Eco130I | CCWWGG | 1 cut(s) | 493 |
| Eco24I | GRGCYC | 1 cut(s) | 396 |
| Eco47I | GGWCC | 2 cut(s) | 270, 1385 |
| Eco57I | CTGAAG | 1 cut(s) | 1443 |
| EcoO109I | RGGNCCY | 3 cut(s) | 143, 213, 1385 |
| EcoRI | GAATTC | 1 cut(s) | 795 |
| EcoRII | CCWGG | 3 cut(s) | 733, 1026, 1477 |
| EcoT14I | CCWWGG | 1 cut(s) | 493 |
| EcoT38I | GRGCYC | 1 cut(s) | 396 |
| ErhI | CCWWGG | 1 cut(s) | 493 |
| Esp3I | CGTCTC | 1 cut(s) | 536 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 848, 880 |
| FaqI | GGGAC | 2 cut(s) | 1302, 1398 |
| FbaI | TGATCA | 1 cut(s) | 1137 |
| Fnu4HI | GCNGC | 3 cut(s) | 646, 649, 1544 |
| FokI | GGATG | 1 cut(s) | 956 |
| FriOI | GRGCYC | 1 cut(s) | 396 |
| Fsp4HI | GCNGC | 3 cut(s) | 646, 649, 1544 |
| FspBI | CTAG | 4 cut(s) | 534, 551, 1211, 1230 |
| GluI | GCNGC | 3 cut(s) | 646, 649, 1544 |
| GsuI | CTGGAG | 1 cut(s) | 870 |
| HaeIII | GGCC | 5 cut(s) | 144, 214, 429, 479, 1519 |
| HindIII | AAGCTT | 1 cut(s) | 37 |
| HphI | GGTGA | 2 cut(s) | 1259, 1459 |
| Hpy166II | GTNNAC | 5 cut(s) | 96, 270, 561, 612, 1489 |
| Hpy188I | TCNGA | 4 cut(s) | 102, 619, 778, 1327 |
| Hpy188III | TCNNGA | 6 cut(s) | 73, 521, 630, 905, 1103, 1238 |
| Hpy8I | GTNNAC | 5 cut(s) | 96, 270, 561, 612, 1489 |
| Hpy99I | CGWCG | 1 cut(s) | 1420 |
| HpyAV | CCTTC | 7 cut(s) | 89, 156, 499, 566, 653, 889, 1322 |
| HpyCH4III | ACNGT | 3 cut(s) | 565, 1018, 1513 |
| HpyCH4IV | ACGT | 2 cut(s) | 968, 1485 |
| HpyCH4V | TGCA | 6 cut(s) | 130, 348, 963, 1300, 1501, 1546 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 485, 1433 |
| HpyF3I | CTNAG | 5 cut(s) | 61, 318, 339, 1154, 1324 |
| HpySE526I | ACGT | 2 cut(s) | 968, 1485 |
| Ksp22I | TGATCA | 1 cut(s) | 1137 |
| Kzo9I | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| LmnI | GCTCC | 1 cut(s) | 57 |
| Lsp1109I | GCAGC | 3 cut(s) | 657, 660, 1530 |
| LweI | GCATC | 1 cut(s) | 537 |
| MaeI | CTAG | 4 cut(s) | 534, 551, 1211, 1230 |
| MaeII | ACGT | 2 cut(s) | 968, 1485 |
| MaeIII | GTNAC | 2 cut(s) | 1180, 1481 |
| MalI | GATC | 4 cut(s) | 77, 137, 1139, 1275 |
| MboI | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| MboII | GAAGA | 6 cut(s) | 23, 287, 298, 421, 608, 1441 |
| MflI | RGATCY | 1 cut(s) | 75 |
| MhlI | GDGCHC | 1 cut(s) | 396 |
| MlsI | TGGCCA | 1 cut(s) | 479 |
| MluCI | AATT | 4 cut(s) | 289, 795, 951, 1241 |
| MluNI | TGGCCA | 1 cut(s) | 479 |
| MlyI | GAGTC | 1 cut(s) | 91 |
| MmeI | TCCRAC | 1 cut(s) | 210 |
| Mox20I | TGGCCA | 1 cut(s) | 479 |
| MscI | TGGCCA | 1 cut(s) | 479 |
| MseI | TTAA | 2 cut(s) | 506, 950 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1359 |
| Msp20I | TGGCCA | 1 cut(s) | 479 |
| MspR9I | CCNGG | 3 cut(s) | 735, 1028, 1479 |
| Mva1269I | GAATGC | 1 cut(s) | 1463 |
| MvaI | CCWGG | 3 cut(s) | 735, 1028, 1479 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 485, 1433 |
| NcoI | CCATGG | 1 cut(s) | 493 |
| NdeII | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| NlaIV | GGNNCC | 3 cut(s) | 215, 732, 1386 |
| NmeAIII | GCCGAG | 1 cut(s) | 565 |
| PagI | TCATGA | 1 cut(s) | 904 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1424 |
| PctI | GAATGC | 1 cut(s) | 1463 |
| PfeI | GAWTC | 9 cut(s) | 103, 156, 517, 696, 738, 773, 908, 1320, 1447 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1526 |
| PfoI | TCCNGGA | 1 cut(s) | 733 |
| PkrI | GCNGC | 3 cut(s) | 647, 650, 1545 |
| PleI | GAGTC | 1 cut(s) | 91 |
| PpsI | GAGTC | 1 cut(s) | 91 |
| PpuMI | RGGWCCY | 1 cut(s) | 1385 |
| Psp5II | RGGWCCY | 1 cut(s) | 1385 |
| Psp6I | CCWGG | 3 cut(s) | 733, 1026, 1477 |
| PspGI | CCWGG | 3 cut(s) | 733, 1026, 1477 |
| PspN4I | GGNNCC | 3 cut(s) | 215, 732, 1386 |
| PspPI | GGNCC | 4 cut(s) | 143, 213, 270, 1385 |
| PspPPI | RGGWCCY | 1 cut(s) | 1385 |
| PstNI | CAGNNNCTG | 2 cut(s) | 338, 358 |
| PsuI | RGATCY | 1 cut(s) | 75 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1359 |
| SaqAI | TTAA | 2 cut(s) | 506, 950 |
| SatI | GCNGC | 3 cut(s) | 646, 649, 1544 |
| Sau3AI | GATC | 4 cut(s) | 75, 135, 1137, 1273 |
| Sau96I | GGNCC | 4 cut(s) | 143, 213, 270, 1385 |
| SchI | GAGTC | 1 cut(s) | 91 |
| ScrFI | CCNGG | 3 cut(s) | 735, 1028, 1479 |
| SduI | GDGCHC | 1 cut(s) | 396 |
| SfaNI | GCATC | 1 cut(s) | 537 |
| SfcI | CTRYAG | 1 cut(s) | 243 |
| SinI | GGWCC | 2 cut(s) | 270, 1385 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1359 |
| SmlI | CTYRAG | 1 cut(s) | 785 |
| SmoI | CTYRAG | 1 cut(s) | 785 |
| Sse9I | AATT | 4 cut(s) | 289, 795, 951, 1241 |
| SspI | AATATT | 2 cut(s) | 705, 715 |
| SspMI | CTAG | 4 cut(s) | 534, 551, 1211, 1230 |
| StyD4I | CCNGG | 3 cut(s) | 733, 1026, 1477 |
| StyI | CCWWGG | 1 cut(s) | 493 |
| TaaI | ACNGT | 3 cut(s) | 565, 1018, 1513 |
| TaiI | ACGT | 2 cut(s) | 971, 1488 |
| TaqI | TCGA | 1 cut(s) | 1390 |
| TasI | AATT | 4 cut(s) | 289, 795, 951, 1241 |
| TfiI | GAWTC | 9 cut(s) | 103, 156, 517, 696, 738, 773, 908, 1320, 1447 |
| Tru1I | TTAA | 2 cut(s) | 506, 950 |
| Tru9I | TTAA | 2 cut(s) | 506, 950 |
| TseI | GCWGC | 3 cut(s) | 645, 648, 1543 |
| TspDTI | ATGAA | 8 cut(s) | 58, 148, 206, 585, 701, 788, 921, 1357 |
| TspGWI | ACGGA | 1 cut(s) | 1055 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1526 |
| VpaK11BI | GGWCC | 2 cut(s) | 270, 1385 |
| XapI | RAATTY | 1 cut(s) | 795 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 491, 814 |
| XspI | CTAG | 4 cut(s) | 534, 551, 1211, 1230 |