Cytochrome p450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 995 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1337 |
| AclWI | GGATC | 2 cut(s) | 322, 1079 |
| AcsI | RAATTY | 2 cut(s) | 262, 569 |
| AcuI | CTGAAG | 2 cut(s) | 570, 1158 |
| AfaI | GTAC | 2 cut(s) | 581, 829 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1288, 1337 |
| AhlI | ACTAGT | 1 cut(s) | 388 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 768, 800 |
| AluBI | AGCT | 9 cut(s) | 12, 38, 368, 436, 445, 503, 621, 783, 1154 |
| AluI | AGCT | 9 cut(s) | 12, 38, 368, 436, 445, 503, 621, 783, 1154 |
| Alw26I | GTCTC | 3 cut(s) | 506, 896, 953 |
| AlwI | GGATC | 2 cut(s) | 322, 1079 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 91, 311 |
| AoxI | GGCC | 2 cut(s) | 48, 185 |
| ApeKI | GCWGC | 3 cut(s) | 615, 618, 1172 |
| ApoI | RAATTY | 2 cut(s) | 262, 569 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 895, 927 |
| AspS9I | GGNCC | 4 cut(s) | 116, 243, 281, 1196 |
| AsuC2I | CCSGG | 1 cut(s) | 1289 |
| AsuHPI | GGTGA | 4 cut(s) | 421, 431, 1070, 1270 |
| AvaII | GGWCC | 4 cut(s) | 116, 243, 281, 1196 |
| BauI | CACGAG | 1 cut(s) | 377 |
| BbsI | GAAGAC | 1 cut(s) | 783 |
| BbvI | GCAGC | 3 cut(s) | 627, 630, 1159 |
| BccI | CCATC | 3 cut(s) | 88, 338, 511 |
| BclI | TGATCA | 1 cut(s) | 42 |
| BcnI | CCSGG | 1 cut(s) | 1289 |
| BcoDI | GTCTC | 3 cut(s) | 506, 896, 953 |
| BcuI | ACTAGT | 1 cut(s) | 388 |
| BfaI | CTAG | 5 cut(s) | 389, 504, 800, 1070, 1200 |
| BfmI | CTRYAG | 1 cut(s) | 101 |
| BisI | GCNGC | 3 cut(s) | 616, 619, 1173 |
| BlsI | GCNGC | 3 cut(s) | 617, 620, 1174 |
| Bme1390I | CCNGG | 1 cut(s) | 1289 |
| Bme18I | GGWCC | 4 cut(s) | 116, 243, 281, 1196 |
| BmgT120I | GGNCC | 4 cut(s) | 116, 243, 281, 1196 |
| BmiI | GGNNCC | 2 cut(s) | 918, 1197 |
| BmrFI | CCNGG | 1 cut(s) | 1289 |
| BmrI | ACTGGG | 1 cut(s) | 1031 |
| BmsI | GCATC | 2 cut(s) | 60, 1096 |
| BmuI | ACTGGG | 1 cut(s) | 1031 |
| BpiI | GAAGAC | 1 cut(s) | 783 |
| BpuMI | CCSGG | 1 cut(s) | 1289 |
| BsaAI | YACGTR | 1 cut(s) | 719 |
| BsaI | GGTCTC | 1 cut(s) | 896 |
| BsaJI | CCNNGG | 2 cut(s) | 463, 1287 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 459, 489 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1288, 1337 |
| Bse1I | ACTGG | 3 cut(s) | 678, 926, 1026 |
| BseDI | CCNNGG | 2 cut(s) | 463, 1287 |
| BseGI | GGATG | 2 cut(s) | 75, 862 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1288, 1337 |
| BseMII | CTCAG | 3 cut(s) | 428, 501, 1325 |
| BseNI | ACTGG | 3 cut(s) | 678, 926, 1026 |
| BseRI | GAGGAG | 2 cut(s) | 770, 884 |
| BseXI | GCAGC | 3 cut(s) | 627, 630, 1159 |
| BsgI | GTGCAG | 1 cut(s) | 895 |
| BshFI | GGCC | 2 cut(s) | 50, 187 |
| BsiSI | CCGG | 1 cut(s) | 1289 |
| BslFI | GGGAC | 3 cut(s) | 1046, 1113, 1209 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1288, 1337 |
| BsmAI | GTCTC | 3 cut(s) | 506, 896, 953 |
| BsmBI | CGTCTC | 1 cut(s) | 506 |
| BsmFI | GGGAC | 3 cut(s) | 1046, 1113, 1209 |
| BsmI | GAATGC | 2 cut(s) | 81, 1175 |
| BsnI | GGCC | 2 cut(s) | 50, 187 |
| Bso31I | GGTCTC | 1 cut(s) | 896 |
| Bsp1407I | TGTACA | 1 cut(s) | 579 |
| Bsp143I | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| BspANI | GGCC | 2 cut(s) | 50, 187 |
| BspCNI | CTCAG | 3 cut(s) | 429, 502, 1324 |
| BspHI | TCATGA | 1 cut(s) | 538 |
| BspLI | GGNNCC | 2 cut(s) | 918, 1197 |
| BspMAI | CTGCAG | 1 cut(s) | 105 |
| BspPI | GGATC | 2 cut(s) | 322, 1079 |
| BspTNI | GGTCTC | 1 cut(s) | 896 |
| BsrGI | TGTACA | 1 cut(s) | 579 |
| BsrI | ACTGG | 3 cut(s) | 678, 926, 1026 |
| BssECI | CCNNGG | 2 cut(s) | 463, 1287 |
| BssMI | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| BssSI | CACGAG | 1 cut(s) | 377 |
| Bst2BI | CACGAG | 1 cut(s) | 377 |
| Bst4CI | ACNGT | 1 cut(s) | 1324 |
| Bst6I | CTCTTC | 3 cut(s) | 217, 747, 1121 |
| BstAUI | TGTACA | 1 cut(s) | 579 |
| BstBAI | YACGTR | 1 cut(s) | 719 |
| BstC8I | GCNNGC | 3 cut(s) | 164, 185, 935 |
| BstDEI | CTNAG | 6 cut(s) | 34, 437, 510, 625, 965, 1311 |
| BstDSI | CCRYGG | 1 cut(s) | 463 |
| BstF5I | GGATG | 2 cut(s) | 75, 862 |
| BstKTI | GATC | 4 cut(s) | 45, 330, 1087, 1245 |
| BstMAI | GTCTC | 3 cut(s) | 506, 896, 953 |
| BstMBI | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 442, 1244 |
| BstNSI | RCATGY | 1 cut(s) | 166 |
| BstSCI | CCNGG | 1 cut(s) | 1287 |
| BstSFI | CTRYAG | 1 cut(s) | 101 |
| BstV1I | GCAGC | 3 cut(s) | 627, 630, 1159 |
| BstV2I | GAAGAC | 1 cut(s) | 783 |
| BstX2I | RGATCY | 1 cut(s) | 327 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 913 |
| BstYI | RGATCY | 1 cut(s) | 327 |
| BsuRI | GGCC | 2 cut(s) | 50, 187 |
| BtgI | CCRYGG | 1 cut(s) | 463 |
| BtgZI | GCGATG | 1 cut(s) | 191 |
| BtsCI | GGATG | 2 cut(s) | 75, 862 |
| BtsIMutI | CAGTG | 2 cut(s) | 685, 1320 |
| Cac8I | GCNNGC | 3 cut(s) | 164, 185, 935 |
| CaiI | CAGNNNCTG | 2 cut(s) | 91, 311 |
| CciI | TCATGA | 1 cut(s) | 538 |
| Cfr13I | GGNCC | 4 cut(s) | 116, 243, 281, 1196 |
| Csp6I | GTAC | 2 cut(s) | 580, 828 |
| CviQI | GTAC | 2 cut(s) | 580, 828 |
| DdeI | CTNAG | 6 cut(s) | 34, 437, 510, 625, 965, 1311 |
| DpnI | GATC | 4 cut(s) | 44, 329, 1086, 1244 |
| DpnII | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| Eam1104I | CTCTTC | 3 cut(s) | 217, 747, 1121 |
| EarI | CTCTTC | 3 cut(s) | 217, 747, 1121 |
| Eco147I | AGGCCT | 1 cut(s) | 50 |
| Eco31I | GGTCTC | 1 cut(s) | 896 |
| Eco32I | GATATC | 1 cut(s) | 208 |
| Eco47I | GGWCC | 4 cut(s) | 116, 243, 281, 1196 |
| Eco57I | CTGAAG | 2 cut(s) | 570, 1158 |
| EcoO109I | RGGNCCY | 2 cut(s) | 116, 1196 |
| EcoRV | GATATC | 1 cut(s) | 208 |
| EcoT22I | ATGCAT | 1 cut(s) | 241 |
| Esp3I | CGTCTC | 1 cut(s) | 506 |
| FaqI | GGGAC | 3 cut(s) | 1046, 1113, 1209 |
| FbaI | TGATCA | 1 cut(s) | 42 |
| Fnu4HI | GCNGC | 3 cut(s) | 616, 619, 1173 |
| FokI | GGATG | 2 cut(s) | 82, 869 |
| Fsp4HI | GCNGC | 3 cut(s) | 616, 619, 1173 |
| FspBI | CTAG | 5 cut(s) | 389, 504, 800, 1070, 1200 |
| GluI | GCNGC | 3 cut(s) | 616, 619, 1173 |
| HaeIII | GGCC | 2 cut(s) | 50, 187 |
| HapII | CCGG | 1 cut(s) | 1289 |
| HindIII | AAGCTT | 2 cut(s) | 10, 366 |
| HinfI | GANTC | 8 cut(s) | 28, 76, 88, 108, 692, 722, 961, 1258 |
| HpaII | CCGG | 1 cut(s) | 1289 |
| HphI | GGTGA | 4 cut(s) | 421, 431, 1070, 1270 |
| Hpy166II | GTNNAC | 3 cut(s) | 284, 582, 1300 |
| Hpy188I | TCNGA | 6 cut(s) | 42, 87, 93, 589, 697, 1138 |
| Hpy188III | TCNNGA | 2 cut(s) | 379, 539 |
| Hpy8I | GTNNAC | 3 cut(s) | 284, 582, 1300 |
| HpyAV | CCTTC | 8 cut(s) | 129, 410, 469, 536, 623, 808, 1133, 1317 |
| HpyCH4III | ACNGT | 1 cut(s) | 1324 |
| HpyCH4IV | ACGT | 2 cut(s) | 718, 881 |
| HpyCH4V | TGCA | 9 cut(s) | 103, 166, 239, 321, 491, 876, 937, 1007, 1175 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 442, 1244 |
| HpyF3I | CTNAG | 6 cut(s) | 34, 437, 510, 625, 965, 1311 |
| HpySE526I | ACGT | 2 cut(s) | 718, 881 |
| Ksp22I | TGATCA | 1 cut(s) | 42 |
| Kzo9I | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| LmnI | GCTCC | 1 cut(s) | 922 |
| Lsp1109I | GCAGC | 3 cut(s) | 627, 630, 1159 |
| LweI | GCATC | 2 cut(s) | 60, 1096 |
| MaeI | CTAG | 5 cut(s) | 389, 504, 800, 1070, 1200 |
| MaeII | ACGT | 2 cut(s) | 718, 881 |
| MaeIII | GTNAC | 3 cut(s) | 301, 409, 719 |
| MalI | GATC | 4 cut(s) | 44, 329, 1086, 1244 |
| MboI | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| MboII | GAAGA | 8 cut(s) | 204, 260, 394, 578, 764, 785, 788, 1108 |
| MflI | RGATCY | 1 cut(s) | 327 |
| MlyI | GAGTC | 3 cut(s) | 70, 716, 955 |
| Mph1103I | ATGCAT | 1 cut(s) | 241 |
| MseI | TTAA | 4 cut(s) | 8, 336, 662, 741 |
| MslI | CAYNNNNRTG | 2 cut(s) | 234, 407 |
| MspI | CCGG | 1 cut(s) | 1289 |
| MspR9I | CCNGG | 1 cut(s) | 1289 |
| Mva1269I | GAATGC | 2 cut(s) | 81, 1175 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 442, 1244 |
| NciI | CCSGG | 1 cut(s) | 1289 |
| NdeII | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| NlaIV | GGNNCC | 2 cut(s) | 918, 1197 |
| NmuCI | GTSAC | 2 cut(s) | 409, 719 |
| NsiI | ATGCAT | 1 cut(s) | 241 |
| NspI | RCATGY | 1 cut(s) | 166 |
| PaeI | GCATGC | 1 cut(s) | 166 |
| PagI | TCATGA | 1 cut(s) | 538 |
| PceI | AGGCCT | 1 cut(s) | 50 |
| PctI | GAATGC | 2 cut(s) | 81, 1175 |
| PfeI | GAWTC | 5 cut(s) | 28, 88, 108, 692, 1258 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1337 |
| PkrI | GCNGC | 3 cut(s) | 617, 620, 1174 |
| PleI | GAGTC | 3 cut(s) | 70, 716, 955 |
| PpsI | GAGTC | 3 cut(s) | 70, 716, 955 |
| Ppu21I | YACGTR | 1 cut(s) | 719 |
| PpuMI | RGGWCCY | 2 cut(s) | 116, 1196 |
| PsiI | TTATAA | 1 cut(s) | 995 |
| Psp5II | RGGWCCY | 2 cut(s) | 116, 1196 |
| PspN4I | GGNNCC | 2 cut(s) | 918, 1197 |
| PspPI | GGNCC | 4 cut(s) | 116, 243, 281, 1196 |
| PspPPI | RGGWCCY | 2 cut(s) | 116, 1196 |
| PstI | CTGCAG | 1 cut(s) | 105 |
| PstNI | CAGNNNCTG | 2 cut(s) | 91, 311 |
| PsuI | RGATCY | 1 cut(s) | 327 |
| RsaI | GTAC | 2 cut(s) | 581, 829 |
| RsaNI | GTAC | 2 cut(s) | 580, 828 |
| RseI | CAYNNNNRTG | 2 cut(s) | 234, 407 |
| SaqAI | TTAA | 4 cut(s) | 8, 336, 662, 741 |
| SatI | GCNGC | 3 cut(s) | 616, 619, 1173 |
| Sau3AI | GATC | 4 cut(s) | 42, 327, 1084, 1242 |
| Sau96I | GGNCC | 4 cut(s) | 116, 243, 281, 1196 |
| SchI | GAGTC | 3 cut(s) | 70, 716, 955 |
| ScrFI | CCNGG | 1 cut(s) | 1289 |
| SfaNI | GCATC | 2 cut(s) | 60, 1096 |
| SfcI | CTRYAG | 1 cut(s) | 101 |
| SinI | GGWCC | 4 cut(s) | 116, 243, 281, 1196 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 234, 407 |
| SpeI | ACTAGT | 1 cut(s) | 388 |
| SphI | GCATGC | 1 cut(s) | 166 |
| SseBI | AGGCCT | 1 cut(s) | 50 |
| SspMI | CTAG | 5 cut(s) | 389, 504, 800, 1070, 1200 |
| StuI | AGGCCT | 1 cut(s) | 50 |
| StyD4I | CCNGG | 1 cut(s) | 1287 |
| TaaI | ACNGT | 1 cut(s) | 1324 |
| TaiI | ACGT | 2 cut(s) | 721, 884 |
| TatI | WGTACW | 1 cut(s) | 579 |
| TfiI | GAWTC | 5 cut(s) | 28, 88, 108, 692, 1258 |
| Tru1I | TTAA | 4 cut(s) | 8, 336, 662, 741 |
| Tru9I | TTAA | 4 cut(s) | 8, 336, 662, 741 |
| TscAI | CASTG | 2 cut(s) | 685, 1327 |
| TseFI | GTSAC | 2 cut(s) | 409, 719 |
| TseI | GCWGC | 3 cut(s) | 615, 618, 1172 |
| Tsp45I | GTSAC | 2 cut(s) | 409, 719 |
| TspDTI | ATGAA | 6 cut(s) | 206, 224, 512, 555, 873, 1168 |
| TspGWI | ACGGA | 1 cut(s) | 452 |
| TspRI | CASTG | 2 cut(s) | 685, 1327 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1337 |
| VpaK11BI | GGWCC | 4 cut(s) | 116, 243, 281, 1196 |
| XapI | RAATTY | 2 cut(s) | 262, 569 |
| XceI | RCATGY | 1 cut(s) | 166 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 461 |
| XspI | CTAG | 5 cut(s) | 389, 504, 800, 1070, 1200 |
| Zsp2I | ATGCAT | 1 cut(s) | 241 |