Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 440 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1547 |
| AccII | CGCG | 1 cut(s) | 1069 |
| AccIII | TCCGGA | 1 cut(s) | 248 |
| AclWI | GGATC | 2 cut(s) | 1238, 1289 |
| AcoI | YGGCCR | 1 cut(s) | 1106 |
| AcsI | RAATTY | 3 cut(s) | 226, 608, 721 |
| AcuI | CTGAAG | 3 cut(s) | 287, 804, 1464 |
| AfaI | GTAC | 3 cut(s) | 46, 983, 1509 |
| AfeI | AGCGCT | 1 cut(s) | 1478 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 1547 |
| AflII | CTTAAG | 1 cut(s) | 1165 |
| AgsI | TTSAA | 7 cut(s) | 27, 79, 686, 910, 1262, 1330, 1556 |
| AjnI | CCWGG | 2 cut(s) | 638, 834 |
| AjuI | GAANNNNNNNTTGG | 6 cut(s) | 482, 514, 710, 742, 864, 896 |
| Alw26I | GTCTC | 1 cut(s) | 773 |
| AlwI | GGATC | 2 cut(s) | 1238, 1289 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 124 |
| Aor13HI | TCCGGA | 1 cut(s) | 248 |
| Aor51HI | AGCGCT | 1 cut(s) | 1478 |
| AoxI | GGCC | 3 cut(s) | 148, 433, 1106 |
| ApeKI | GCWGC | 3 cut(s) | 14, 654, 657 |
| ApoI | RAATTY | 3 cut(s) | 226, 608, 721 |
| AseI | ATTAAT | 1 cut(s) | 1155 |
| AspA2I | CCTAGG | 1 cut(s) | 802 |
| AspLEI | GCGC | 3 cut(s) | 1069, 1479, 1528 |
| AspS9I | GGNCC | 3 cut(s) | 149, 276, 1023 |
| AsuHPI | GGTGA | 2 cut(s) | 343, 1480 |
| AvaII | GGWCC | 2 cut(s) | 276, 1023 |
| AvrII | CCTAGG | 1 cut(s) | 802 |
| BaeGI | GKGCMC | 1 cut(s) | 472 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 36, 69 |
| BalI | TGGCCA | 1 cut(s) | 1108 |
| BanI | GGYRCC | 1 cut(s) | 440 |
| BbvI | GCAGC | 2 cut(s) | 666, 669 |
| BccI | CCATC | 2 cut(s) | 1034, 1112 |
| BciT130I | CCWGG | 2 cut(s) | 640, 836 |
| BciVI | GTATCC | 1 cut(s) | 256 |
| BclI | TGATCA | 2 cut(s) | 3, 1149 |
| BcoDI | GTCTC | 1 cut(s) | 773 |
| BfaI | CTAG | 4 cut(s) | 744, 803, 1037, 1379 |
| BfmI | CTRYAG | 2 cut(s) | 624, 1472 |
| BfoI | RGCGCY | 2 cut(s) | 1480, 1529 |
| BfrI | CTTAAG | 1 cut(s) | 1165 |
| BfuI | GTATCC | 1 cut(s) | 256 |
| BglII | AGATCT | 1 cut(s) | 483 |
| BisI | GCNGC | 3 cut(s) | 15, 655, 658 |
| BlnI | CCTAGG | 1 cut(s) | 802 |
| BlsI | GCNGC | 3 cut(s) | 16, 656, 659 |
| Bme1390I | CCNGG | 2 cut(s) | 640, 836 |
| Bme18I | GGWCC | 2 cut(s) | 276, 1023 |
| BmgT120I | GGNCC | 3 cut(s) | 149, 276, 1023 |
| BmiI | GGNNCC | 2 cut(s) | 399, 442 |
| BmrFI | CCNGG | 2 cut(s) | 640, 836 |
| BmsI | GCATC | 5 cut(s) | 546, 756, 913, 1259, 1330 |
| BpmI | CTGGAG | 1 cut(s) | 885 |
| Bpu10I | CCTNAGC | 1 cut(s) | 135 |
| BsaBI | GATNNNNATC | 2 cut(s) | 8, 1320 |
| BsaJI | CCNNGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| BsaWI | WCCGGW | 1 cut(s) | 248 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1480, 1510 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 1547 |
| Bse1I | ACTGG | 4 cut(s) | 52, 868, 1008, 1109 |
| Bse3DI | GCAATG | 3 cut(s) | 861, 1122, 1283 |
| Bse8I | GATNNNNATC | 2 cut(s) | 8, 1320 |
| BseAI | TCCGGA | 1 cut(s) | 248 |
| BseBI | CCWGG | 2 cut(s) | 640, 836 |
| BseDI | CCNNGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| BseGI | GGATG | 4 cut(s) | 165, 256, 831, 1026 |
| BseJI | GATNNNNATC | 2 cut(s) | 8, 1320 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 1547 |
| BseMI | GCAATG | 3 cut(s) | 861, 1122, 1283 |
| BseMII | CTCAG | 3 cut(s) | 149, 332, 338 |
| BseNI | ACTGG | 4 cut(s) | 52, 868, 1008, 1109 |
| BseRI | GAGGAG | 1 cut(s) | 1170 |
| BseSI | GKGCMC | 1 cut(s) | 472 |
| BseXI | GCAGC | 2 cut(s) | 666, 669 |
| BseYI | CCCAGC | 1 cut(s) | 548 |
| Bsh1236I | CGCG | 1 cut(s) | 1069 |
| BshFI | GGCC | 3 cut(s) | 150, 435, 1108 |
| BshNI | GGYRCC | 1 cut(s) | 440 |
| BsiSI | CCGG | 1 cut(s) | 249 |
| BslFI | GGGAC | 2 cut(s) | 565, 1323 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 1547 |
| BsmAI | GTCTC | 1 cut(s) | 773 |
| BsmFI | GGGAC | 2 cut(s) | 565, 1323 |
| BsmI | GAATGC | 1 cut(s) | 114 |
| BsnI | GGCC | 3 cut(s) | 150, 435, 1108 |
| Bsp1286I | GDGCHC | 1 cut(s) | 472 |
| Bsp13I | TCCGGA | 1 cut(s) | 248 |
| Bsp1407I | TGTACA | 1 cut(s) | 1507 |
| Bsp143I | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| Bsp19I | CCATGG | 1 cut(s) | 169 |
| BspANI | GGCC | 3 cut(s) | 150, 435, 1108 |
| BspCNI | CTCAG | 3 cut(s) | 148, 331, 337 |
| BspEI | TCCGGA | 1 cut(s) | 248 |
| BspFNI | CGCG | 1 cut(s) | 1069 |
| BspHI | TCATGA | 2 cut(s) | 919, 1114 |
| BspLI | GGNNCC | 2 cut(s) | 399, 442 |
| BspMAI | CTGCAG | 1 cut(s) | 628 |
| BspPI | GGATC | 2 cut(s) | 1238, 1289 |
| BspT107I | GGYRCC | 1 cut(s) | 440 |
| BspTI | CTTAAG | 1 cut(s) | 1165 |
| BsrDI | GCAATG | 3 cut(s) | 861, 1122, 1283 |
| BsrGI | TGTACA | 1 cut(s) | 1507 |
| BsrI | ACTGG | 4 cut(s) | 52, 868, 1008, 1109 |
| BssECI | CCNNGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| BssMI | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| BssT1I | CCWWGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| Bst2UI | CCWGG | 2 cut(s) | 640, 836 |
| Bst4CI | ACNGT | 2 cut(s) | 949, 1192 |
| Bst6I | CTCTTC | 1 cut(s) | 1331 |
| BstAFI | CTTAAG | 1 cut(s) | 1165 |
| BstAUI | TGTACA | 1 cut(s) | 1507 |
| BstDEI | CTNAG | 4 cut(s) | 135, 318, 324, 664 |
| BstDSI | CCRYGG | 1 cut(s) | 169 |
| BstF5I | GGATG | 4 cut(s) | 165, 256, 831, 1026 |
| BstFNI | CGCG | 1 cut(s) | 1069 |
| BstH2I | RGCGCY | 2 cut(s) | 1480, 1529 |
| BstHHI | GCGC | 3 cut(s) | 1069, 1479, 1528 |
| BstKTI | GATC | 8 cut(s) | 6, 75, 189, 486, 831, 1152, 1246, 1297 |
| BstMAI | GTCTC | 1 cut(s) | 773 |
| BstMBI | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| BstNI | CCWGG | 2 cut(s) | 640, 836 |
| BstSCI | CCNGG | 2 cut(s) | 638, 834 |
| BstSFI | CTRYAG | 2 cut(s) | 624, 1472 |
| BstSLI | GKGCMC | 1 cut(s) | 472 |
| BstUI | CGCG | 1 cut(s) | 1069 |
| BstV1I | GCAGC | 2 cut(s) | 666, 669 |
| BstX2I | RGATCY | 1 cut(s) | 483 |
| BstYI | RGATCY | 1 cut(s) | 483 |
| BsuI | GTATCC | 1 cut(s) | 256 |
| BsuRI | GGCC | 3 cut(s) | 150, 435, 1108 |
| BtgI | CCRYGG | 1 cut(s) | 169 |
| BtsCI | GGATG | 4 cut(s) | 165, 256, 831, 1026 |
| BtsI | GCAGTG | 1 cut(s) | 1002 |
| BtsIMutI | CAGTG | 3 cut(s) | 311, 1002, 1440 |
| CaiI | CAGNNNCTG | 1 cut(s) | 124 |
| CciI | TCATGA | 2 cut(s) | 919, 1114 |
| CfoI | GCGC | 3 cut(s) | 1069, 1479, 1528 |
| Cfr13I | GGNCC | 3 cut(s) | 149, 276, 1023 |
| Csp6I | GTAC | 3 cut(s) | 45, 982, 1508 |
| CviQI | GTAC | 3 cut(s) | 45, 982, 1508 |
| DdeI | CTNAG | 4 cut(s) | 135, 318, 324, 664 |
| DpnI | GATC | 8 cut(s) | 5, 74, 188, 485, 830, 1151, 1245, 1296 |
| DpnII | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| EaeI | YGGCCR | 1 cut(s) | 1106 |
| Eam1104I | CTCTTC | 1 cut(s) | 1331 |
| EarI | CTCTTC | 1 cut(s) | 1331 |
| Eco130I | CCWWGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| Eco147I | AGGCCT | 1 cut(s) | 435 |
| Eco47I | GGWCC | 2 cut(s) | 276, 1023 |
| Eco47III | AGCGCT | 1 cut(s) | 1478 |
| Eco57I | CTGAAG | 3 cut(s) | 287, 804, 1464 |
| EcoO109I | RGGNCCY | 1 cut(s) | 149 |
| EcoRII | CCWGG | 2 cut(s) | 638, 834 |
| EcoT14I | CCWWGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| ErhI | CCWWGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 407, 439, 689, 721 |
| FaqI | GGGAC | 2 cut(s) | 565, 1323 |
| FbaI | TGATCA | 2 cut(s) | 3, 1149 |
| Fnu4HI | GCNGC | 3 cut(s) | 15, 655, 658 |
| FokI | GGATG | 4 cut(s) | 152, 243, 818, 1013 |
| Fsp4HI | GCNGC | 3 cut(s) | 15, 655, 658 |
| FspBI | CTAG | 4 cut(s) | 744, 803, 1037, 1379 |
| GlaI | GCGC | 3 cut(s) | 1068, 1478, 1527 |
| GluI | GCNGC | 3 cut(s) | 15, 655, 658 |
| GsaI | CCCAGC | 1 cut(s) | 552 |
| GsuI | CTGGAG | 1 cut(s) | 885 |
| HaeII | RGCGCY | 2 cut(s) | 1480, 1529 |
| HaeIII | GGCC | 3 cut(s) | 150, 435, 1108 |
| HapII | CCGG | 1 cut(s) | 249 |
| HhaI | GCGC | 3 cut(s) | 1069, 1479, 1528 |
| Hin6I | GCGC | 3 cut(s) | 1067, 1477, 1526 |
| HinP1I | GCGC | 3 cut(s) | 1067, 1477, 1526 |
| HincII | GTYRAC | 2 cut(s) | 621, 934 |
| HindII | GTYRAC | 2 cut(s) | 621, 934 |
| HindIII | AAGCTT | 1 cut(s) | 794 |
| HpaII | CCGG | 1 cut(s) | 249 |
| HphI | GGTGA | 2 cut(s) | 343, 1480 |
| Hpy166II | GTNNAC | 6 cut(s) | 102, 621, 763, 934, 1013, 1510 |
| Hpy188I | TCNGA | 6 cut(s) | 72, 120, 146, 390, 457, 1400 |
| Hpy188III | TCNNGA | 6 cut(s) | 249, 415, 920, 1115, 1259, 1298 |
| Hpy8I | GTNNAC | 6 cut(s) | 102, 621, 763, 934, 1013, 1510 |
| HpyCH4III | ACNGT | 2 cut(s) | 949, 1192 |
| HpyCH4V | TGCA | 5 cut(s) | 626, 972, 989, 1288, 1321 |
| HpyF3I | CTNAG | 4 cut(s) | 135, 318, 324, 664 |
| HspAI | GCGC | 3 cut(s) | 1067, 1477, 1526 |
| Kpn2I | TCCGGA | 1 cut(s) | 248 |
| Ksp22I | TGATCA | 2 cut(s) | 3, 1149 |
| Kzo9I | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| LmnI | GCTCC | 2 cut(s) | 397, 564 |
| Lsp1109I | GCAGC | 2 cut(s) | 666, 669 |
| LweI | GCATC | 5 cut(s) | 546, 756, 913, 1259, 1330 |
| MaeI | CTAG | 4 cut(s) | 744, 803, 1037, 1379 |
| MaeIII | GTNAC | 2 cut(s) | 812, 1186 |
| MalI | GATC | 8 cut(s) | 5, 74, 188, 485, 830, 1151, 1245, 1296 |
| MboI | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| MboII | GAAGA | 9 cut(s) | 67, 293, 304, 617, 838, 881, 1025, 1318, 1422 |
| MfeI | CAATTG | 1 cut(s) | 473 |
| MflI | RGATCY | 1 cut(s) | 483 |
| MhlI | GDGCHC | 1 cut(s) | 472 |
| MlsI | TGGCCA | 1 cut(s) | 1108 |
| MluCI | AATT | 7 cut(s) | 57, 226, 295, 473, 608, 721, 956 |
| MluNI | TGGCCA | 1 cut(s) | 1108 |
| MlyI | GAGTC | 3 cut(s) | 97, 809, 1169 |
| MmeI | TCCRAC | 3 cut(s) | 131, 513, 1523 |
| Mox20I | TGGCCA | 1 cut(s) | 1108 |
| MroI | TCCGGA | 1 cut(s) | 248 |
| MscI | TGGCCA | 1 cut(s) | 1108 |
| MseI | TTAA | 4 cut(s) | 515, 710, 1155, 1166 |
| MslI | CAYNNNNRTG | 1 cut(s) | 921 |
| Msp20I | TGGCCA | 1 cut(s) | 1108 |
| MspA1I | CMGCKG | 1 cut(s) | 1208 |
| MspCI | CTTAAG | 1 cut(s) | 1165 |
| MspI | CCGG | 1 cut(s) | 249 |
| MspR9I | CCNGG | 2 cut(s) | 640, 836 |
| MunI | CAATTG | 1 cut(s) | 473 |
| Mva1269I | GAATGC | 1 cut(s) | 114 |
| MvaI | CCWGG | 2 cut(s) | 640, 836 |
| MvnI | CGCG | 1 cut(s) | 1069 |
| NcoI | CCATGG | 1 cut(s) | 169 |
| NdeII | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| NlaIV | GGNNCC | 2 cut(s) | 399, 442 |
| NmuCI | GTSAC | 1 cut(s) | 812 |
| PagI | TCATGA | 2 cut(s) | 919, 1114 |
| PceI | AGGCCT | 1 cut(s) | 435 |
| PctI | GAATGC | 1 cut(s) | 114 |
| PfeI | GAWTC | 8 cut(s) | 141, 162, 526, 807, 998, 1341, 1386, 1468 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1547 |
| PfoI | TCCNGGA | 2 cut(s) | 638, 834 |
| PkrI | GCNGC | 3 cut(s) | 16, 656, 659 |
| PleI | GAGTC | 3 cut(s) | 97, 809, 1168 |
| PpsI | GAGTC | 3 cut(s) | 97, 809, 1168 |
| PshBI | ATTAAT | 1 cut(s) | 1155 |
| Psp6I | CCWGG | 2 cut(s) | 638, 834 |
| PspFI | CCCAGC | 1 cut(s) | 548 |
| PspGI | CCWGG | 2 cut(s) | 638, 834 |
| PspN4I | GGNNCC | 2 cut(s) | 399, 442 |
| PspPI | GGNCC | 3 cut(s) | 149, 276, 1023 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 237, 269 |
| PstI | CTGCAG | 1 cut(s) | 628 |
| PstNI | CAGNNNCTG | 1 cut(s) | 124 |
| PsuI | RGATCY | 1 cut(s) | 483 |
| PvuII | CAGCTG | 1 cut(s) | 1208 |
| RsaI | GTAC | 3 cut(s) | 46, 983, 1509 |
| RsaNI | GTAC | 3 cut(s) | 45, 982, 1508 |
| RseI | CAYNNNNRTG | 1 cut(s) | 921 |
| SaqAI | TTAA | 4 cut(s) | 515, 710, 1155, 1166 |
| SatI | GCNGC | 3 cut(s) | 15, 655, 658 |
| Sau3AI | GATC | 8 cut(s) | 3, 72, 186, 483, 828, 1149, 1243, 1294 |
| Sau96I | GGNCC | 3 cut(s) | 149, 276, 1023 |
| SchI | GAGTC | 3 cut(s) | 97, 809, 1169 |
| ScrFI | CCNGG | 2 cut(s) | 640, 836 |
| SduI | GDGCHC | 1 cut(s) | 472 |
| SfaNI | GCATC | 5 cut(s) | 546, 756, 913, 1259, 1330 |
| SfcI | CTRYAG | 2 cut(s) | 624, 1472 |
| SinI | GGWCC | 2 cut(s) | 276, 1023 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 921 |
| SmlI | CTYRAG | 1 cut(s) | 1165 |
| SmoI | CTYRAG | 1 cut(s) | 1165 |
| Sse9I | AATT | 7 cut(s) | 57, 226, 295, 473, 608, 721, 956 |
| SseBI | AGGCCT | 1 cut(s) | 435 |
| SspMI | CTAG | 4 cut(s) | 744, 803, 1037, 1379 |
| StuI | AGGCCT | 1 cut(s) | 435 |
| StyD4I | CCNGG | 2 cut(s) | 638, 834 |
| StyI | CCWWGG | 5 cut(s) | 169, 436, 502, 802, 849 |
| TaaI | ACNGT | 2 cut(s) | 949, 1192 |
| TaqI | TCGA | 1 cut(s) | 1428 |
| TasI | AATT | 7 cut(s) | 57, 226, 295, 473, 608, 721, 956 |
| TatI | WGTACW | 2 cut(s) | 981, 1507 |
| TfiI | GAWTC | 8 cut(s) | 141, 162, 526, 807, 998, 1341, 1386, 1468 |
| Tru1I | TTAA | 4 cut(s) | 515, 710, 1155, 1166 |
| Tru9I | TTAA | 4 cut(s) | 515, 710, 1155, 1166 |
| TscAI | CASTG | 3 cut(s) | 311, 1009, 1447 |
| TseFI | GTSAC | 1 cut(s) | 812 |
| TseI | GCWGC | 3 cut(s) | 14, 654, 657 |
| Tsp45I | GTSAC | 1 cut(s) | 812 |
| TspDTI | ATGAA | 9 cut(s) | 154, 212, 245, 474, 594, 618, 710, 1034, 1077 |
| TspGWI | ACGGA | 1 cut(s) | 800 |
| TspRI | CASTG | 3 cut(s) | 311, 1009, 1447 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1547 |
| Vha464I | CTTAAG | 1 cut(s) | 1165 |
| VpaK11BI | GGWCC | 2 cut(s) | 276, 1023 |
| VspI | ATTAAT | 1 cut(s) | 1155 |
| XapI | RAATTY | 3 cut(s) | 226, 608, 721 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 500 |
| XmaJI | CCTAGG | 1 cut(s) | 802 |
| XspI | CTAG | 4 cut(s) | 744, 803, 1037, 1379 |