Belongs to the cytochrome P450 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 23 |
| AccB1I | GGYRCC | 1 cut(s) | 1250 |
| AccBSI | CCGCTC | 1 cut(s) | 801 |
| AccII | CGCG | 1 cut(s) | 430 |
| AciI | CCGC | 2 cut(s) | 430, 801 |
| AclWI | GGATC | 2 cut(s) | 830, 1276 |
| AcoI | YGGCCR | 1 cut(s) | 1194 |
| AcsI | RAATTY | 3 cut(s) | 256, 560, 698 |
| AcuI | CTGAAG | 3 cut(s) | 248, 894, 1278 |
| AfaI | GTAC | 3 cut(s) | 161, 207, 277 |
| AfeI | AGCGCT | 1 cut(s) | 1292 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 886 |
| AjiI | CACGTC | 1 cut(s) | 360 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 801, 833 |
| Alw21I | GWGCWC | 1 cut(s) | 286 |
| Alw26I | GTCTC | 4 cut(s) | 497, 840, 920, 1314 |
| AlwI | GGATC | 2 cut(s) | 830, 1276 |
| Ama87I | CYCGRG | 1 cut(s) | 279 |
| Aor51HI | AGCGCT | 1 cut(s) | 1292 |
| AoxI | GGCC | 4 cut(s) | 391, 535, 1194, 1237 |
| ApeKI | GCWGC | 3 cut(s) | 606, 609, 1339 |
| ApoI | RAATTY | 3 cut(s) | 256, 560, 698 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 344, 376 |
| AspA2I | CCTAGG | 1 cut(s) | 1223 |
| AspLEI | GCGC | 2 cut(s) | 1293, 1353 |
| AspS9I | GGNCC | 1 cut(s) | 1220 |
| AsuC2I | CCSGG | 1 cut(s) | 1314 |
| AsuHPI | GGTGA | 1 cut(s) | 422 |
| AvaI | CYCGRG | 1 cut(s) | 279 |
| AvaII | GGWCC | 1 cut(s) | 1220 |
| AvrII | CCTAGG | 1 cut(s) | 1223 |
| BalI | TGGCCA | 1 cut(s) | 1196 |
| BanI | GGYRCC | 1 cut(s) | 1250 |
| BanII | GRGCYC | 1 cut(s) | 286 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 720, 752 |
| Bbv12I | GWGCWC | 1 cut(s) | 286 |
| BbvI | GCAGC | 3 cut(s) | 618, 621, 1351 |
| BccI | CCATC | 2 cut(s) | 332, 513 |
| BceAI | ACGGC | 1 cut(s) | 1373 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 260, 294 |
| BclI | TGATCA | 1 cut(s) | 972 |
| BcnI | CCSGG | 1 cut(s) | 1314 |
| BcoDI | GTCTC | 4 cut(s) | 497, 840, 920, 1314 |
| BfaI | CTAG | 6 cut(s) | 365, 495, 512, 591, 1094, 1224 |
| BfmI | CTRYAG | 3 cut(s) | 210, 576, 1286 |
| BfoI | RGCGCY | 1 cut(s) | 1294 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1336 |
| BisI | GCNGC | 3 cut(s) | 607, 610, 1340 |
| BlnI | CCTAGG | 1 cut(s) | 1223 |
| BlsI | GCNGC | 3 cut(s) | 608, 611, 1341 |
| Bme1390I | CCNGG | 1 cut(s) | 1314 |
| Bme18I | GGWCC | 1 cut(s) | 1220 |
| BmeT110I | CYCGRG | 1 cut(s) | 279 |
| BmgBI | CACGTC | 1 cut(s) | 360 |
| BmgT120I | GGNCC | 1 cut(s) | 1220 |
| BmiI | GGNNCC | 3 cut(s) | 303, 1221, 1252 |
| BmrFI | CCNGG | 1 cut(s) | 1314 |
| BmsI | GCATC | 1 cut(s) | 699 |
| BpuEI | CTTGAG | 2 cut(s) | 1009, 1354 |
| BpuMI | CCSGG | 1 cut(s) | 1314 |
| BsaI | GGTCTC | 1 cut(s) | 840 |
| BsaJI | CCNNGG | 3 cut(s) | 454, 1223, 1355 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 97, 127, 1294, 1324 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 886 |
| Bse3DI | GCAATG | 1 cut(s) | 801 |
| BseDI | CCNNGG | 3 cut(s) | 454, 1223, 1355 |
| BseGI | GGATG | 1 cut(s) | 1083 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 886 |
| BseMI | GCAATG | 1 cut(s) | 801 |
| BseMII | CTCAG | 3 cut(s) | 299, 492, 1173 |
| BseXI | GCAGC | 3 cut(s) | 618, 621, 1351 |
| Bsh1236I | CGCG | 1 cut(s) | 430 |
| BshFI | GGCC | 4 cut(s) | 393, 537, 1196, 1239 |
| BshNI | GGYRCC | 1 cut(s) | 1250 |
| BsiHKAI | GWGCWC | 1 cut(s) | 286 |
| BsiHKCI | CYCGRG | 1 cut(s) | 279 |
| BsiSI | CCGG | 1 cut(s) | 1313 |
| BslFI | GGGAC | 3 cut(s) | 346, 1137, 1233 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 886 |
| BsmAI | GTCTC | 4 cut(s) | 497, 840, 920, 1314 |
| BsmFI | GGGAC | 3 cut(s) | 346, 1137, 1233 |
| BsnI | GGCC | 4 cut(s) | 393, 537, 1196, 1239 |
| Bso31I | GGTCTC | 1 cut(s) | 840 |
| BsoBI | CYCGRG | 1 cut(s) | 279 |
| Bsp1286I | GDGCHC | 1 cut(s) | 286 |
| Bsp1407I | TGTACA | 1 cut(s) | 159 |
| Bsp143I | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| Bsp19I | CCATGG | 1 cut(s) | 454 |
| BspACI | CCGC | 2 cut(s) | 430, 801 |
| BspANI | GGCC | 4 cut(s) | 393, 537, 1196, 1239 |
| BspCNI | CTCAG | 3 cut(s) | 298, 493, 1172 |
| BspFNI | CGCG | 1 cut(s) | 430 |
| BspLI | GGNNCC | 3 cut(s) | 303, 1221, 1252 |
| BspMAI | CTGCAG | 1 cut(s) | 580 |
| BspPI | GGATC | 2 cut(s) | 830, 1276 |
| BspT107I | GGYRCC | 1 cut(s) | 1250 |
| BspTNI | GGTCTC | 1 cut(s) | 840 |
| BsrBI | CCGCTC | 1 cut(s) | 801 |
| BsrDI | GCAATG | 1 cut(s) | 801 |
| BsrGI | TGTACA | 1 cut(s) | 159 |
| BssECI | CCNNGG | 3 cut(s) | 454, 1223, 1355 |
| BssMI | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| BssT1I | CCWWGG | 2 cut(s) | 454, 1223 |
| Bst4CI | ACNGT | 2 cut(s) | 851, 1039 |
| Bst6I | CTCTTC | 3 cut(s) | 56, 780, 804 |
| BstAUI | TGTACA | 1 cut(s) | 159 |
| BstC8I | GCNNGC | 3 cut(s) | 179, 438, 1344 |
| BstDEI | CTNAG | 4 cut(s) | 184, 285, 501, 1159 |
| BstDSI | CCRYGG | 2 cut(s) | 454, 1355 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 884 |
| BstF5I | GGATG | 1 cut(s) | 1083 |
| BstFNI | CGCG | 1 cut(s) | 430 |
| BstH2I | RGCGCY | 1 cut(s) | 1294 |
| BstHHI | GCGC | 2 cut(s) | 1293, 1353 |
| BstKTI | GATC | 6 cut(s) | 72, 670, 723, 825, 975, 1284 |
| BstMAI | GTCTC | 4 cut(s) | 497, 840, 920, 1314 |
| BstMBI | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 446, 1268, 1336, 1348, 1357, 1384 |
| BstSCI | CCNGG | 1 cut(s) | 1312 |
| BstSFI | CTRYAG | 3 cut(s) | 210, 576, 1286 |
| BstUI | CGCG | 1 cut(s) | 430 |
| BstV1I | GCAGC | 3 cut(s) | 618, 621, 1351 |
| BsuRI | GGCC | 4 cut(s) | 393, 537, 1196, 1239 |
| BtgI | CCRYGG | 2 cut(s) | 454, 1355 |
| BtrI | CACGTC | 1 cut(s) | 360 |
| BtsCI | GGATG | 1 cut(s) | 1083 |
| BtsI | GCAGTG | 1 cut(s) | 1346 |
| BtsIMutI | CAGTG | 3 cut(s) | 272, 1254, 1346 |
| Cac8I | GCNNGC | 3 cut(s) | 179, 438, 1344 |
| CfoI | GCGC | 2 cut(s) | 1293, 1353 |
| Cfr13I | GGNCC | 1 cut(s) | 1220 |
| CseI | GACGC | 1 cut(s) | 1139 |
| Csp6I | GTAC | 3 cut(s) | 160, 206, 276 |
| CviQI | GTAC | 3 cut(s) | 160, 206, 276 |
| DdeI | CTNAG | 4 cut(s) | 184, 285, 501, 1159 |
| DpnI | GATC | 6 cut(s) | 71, 669, 722, 824, 974, 1283 |
| DpnII | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 23 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 23 |
| EaeI | YGGCCR | 1 cut(s) | 1194 |
| Eam1104I | CTCTTC | 3 cut(s) | 56, 780, 804 |
| EarI | CTCTTC | 3 cut(s) | 56, 780, 804 |
| Ecl136II | GAGCTC | 1 cut(s) | 284 |
| Eco130I | CCWWGG | 2 cut(s) | 454, 1223 |
| Eco147I | AGGCCT | 1 cut(s) | 1239 |
| Eco24I | GRGCYC | 1 cut(s) | 286 |
| Eco31I | GGTCTC | 1 cut(s) | 840 |
| Eco47I | GGWCC | 1 cut(s) | 1220 |
| Eco47III | AGCGCT | 1 cut(s) | 1292 |
| Eco53kI | GAGCTC | 1 cut(s) | 284 |
| Eco57I | CTGAAG | 3 cut(s) | 248, 894, 1278 |
| Eco88I | CYCGRG | 1 cut(s) | 279 |
| EcoICRI | GAGCTC | 1 cut(s) | 284 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 884 |
| EcoO109I | RGGNCCY | 1 cut(s) | 1220 |
| EcoT14I | CCWWGG | 2 cut(s) | 454, 1223 |
| EcoT22I | ATGCAT | 2 cut(s) | 132, 235 |
| EcoT38I | GRGCYC | 1 cut(s) | 286 |
| ErhI | CCWWGG | 2 cut(s) | 454, 1223 |
| FaqI | GGGAC | 3 cut(s) | 346, 1137, 1233 |
| FauI | CCCGC | 1 cut(s) | 794 |
| FbaI | TGATCA | 1 cut(s) | 972 |
| Fnu4HI | GCNGC | 3 cut(s) | 607, 610, 1340 |
| FokI | GGATG | 1 cut(s) | 1070 |
| FriOI | GRGCYC | 1 cut(s) | 286 |
| Fsp4HI | GCNGC | 3 cut(s) | 607, 610, 1340 |
| FspBI | CTAG | 6 cut(s) | 365, 495, 512, 591, 1094, 1224 |
| GlaI | GCGC | 2 cut(s) | 1292, 1352 |
| GluI | GCNGC | 3 cut(s) | 607, 610, 1340 |
| HaeII | RGCGCY | 1 cut(s) | 1294 |
| HaeIII | GGCC | 4 cut(s) | 393, 537, 1196, 1239 |
| HapII | CCGG | 1 cut(s) | 1313 |
| HgaI | GACGC | 1 cut(s) | 1139 |
| HhaI | GCGC | 2 cut(s) | 1293, 1353 |
| Hin6I | GCGC | 2 cut(s) | 1291, 1351 |
| HinP1I | GCGC | 2 cut(s) | 1291, 1351 |
| HincII | GTYRAC | 2 cut(s) | 573, 661 |
| HindII | GTYRAC | 2 cut(s) | 573, 661 |
| HindIII | AAGCTT | 1 cut(s) | 734 |
| HinfI | GANTC | 6 cut(s) | 100, 478, 747, 928, 983, 1155 |
| HpaII | CCGG | 1 cut(s) | 1313 |
| HphI | GGTGA | 1 cut(s) | 422 |
| Hpy166II | GTNNAC | 3 cut(s) | 573, 661, 1324 |
| Hpy188I | TCNGA | 3 cut(s) | 69, 741, 1162 |
| Hpy188III | TCNNGA | 4 cut(s) | 295, 557, 872, 919 |
| Hpy8I | GTNNAC | 3 cut(s) | 573, 661, 1324 |
| HpyAV | CCTTC | 7 cut(s) | 401, 404, 460, 784, 1121, 1157, 1250 |
| HpyCH4III | ACNGT | 2 cut(s) | 851, 1039 |
| HpyCH4IV | ACGT | 2 cut(s) | 359, 1014 |
| HpyCH4V | TGCA | 8 cut(s) | 130, 196, 233, 578, 619, 753, 883, 910 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 446, 1268, 1336, 1348, 1357, 1384 |
| HpyF3I | CTNAG | 4 cut(s) | 184, 285, 501, 1159 |
| HpySE526I | ACGT | 2 cut(s) | 359, 1014 |
| HspAI | GCGC | 2 cut(s) | 1291, 1351 |
| Ksp22I | TGATCA | 1 cut(s) | 972 |
| Kzo9I | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| LmnI | GCTCC | 3 cut(s) | 237, 341, 653 |
| Lsp1109I | GCAGC | 3 cut(s) | 618, 621, 1351 |
| LweI | GCATC | 1 cut(s) | 699 |
| MaeI | CTAG | 6 cut(s) | 365, 495, 512, 591, 1094, 1224 |
| MaeII | ACGT | 2 cut(s) | 359, 1014 |
| MaeIII | GTNAC | 3 cut(s) | 295, 314, 1015 |
| MalI | GATC | 6 cut(s) | 71, 669, 722, 824, 974, 1283 |
| MbiI | CCGCTC | 1 cut(s) | 801 |
| MboI | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| MboII | GAAGA | 8 cut(s) | 43, 64, 254, 265, 528, 797, 818, 821 |
| MhlI | GDGCHC | 1 cut(s) | 286 |
| MlsI | TGGCCA | 1 cut(s) | 1196 |
| MluCI | AATT | 9 cut(s) | 256, 308, 381, 560, 698, 861, 911, 1115, 1228 |
| MluNI | TGGCCA | 1 cut(s) | 1196 |
| MlyI | GAGTC | 1 cut(s) | 922 |
| MnlI | CCTC | 6 cut(s) | 123, 231, 541, 880, 984, 1329 |
| Mox20I | TGGCCA | 1 cut(s) | 1196 |
| Mph1103I | ATGCAT | 2 cut(s) | 132, 235 |
| MscI | TGGCCA | 1 cut(s) | 1196 |
| MseI | TTAA | 7 cut(s) | 467, 635, 644, 705, 774, 860, 1079 |
| MslI | CAYNNNNRTG | 2 cut(s) | 125, 228 |
| Msp20I | TGGCCA | 1 cut(s) | 1196 |
| MspA1I | CMGCKG | 1 cut(s) | 1342 |
| MspI | CCGG | 1 cut(s) | 1313 |
| MspR9I | CCNGG | 1 cut(s) | 1314 |
| MvnI | CGCG | 1 cut(s) | 430 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 446, 1268, 1336, 1348, 1357, 1384 |
| NciI | CCSGG | 1 cut(s) | 1314 |
| NcoI | CCATGG | 1 cut(s) | 454 |
| NdeII | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| NlaIV | GGNNCC | 3 cut(s) | 303, 1221, 1252 |
| NmuCI | GTSAC | 1 cut(s) | 295 |
| NsiI | ATGCAT | 2 cut(s) | 132, 235 |
| PaeR7I | CTCGAG | 1 cut(s) | 279 |
| PceI | AGGCCT | 1 cut(s) | 1239 |
| PfeI | GAWTC | 5 cut(s) | 100, 478, 747, 983, 1155 |
| PkrI | GCNGC | 3 cut(s) | 608, 611, 1341 |
| PleI | GAGTC | 1 cut(s) | 922 |
| PpsI | GAGTC | 1 cut(s) | 922 |
| PpuMI | RGGWCCY | 1 cut(s) | 1220 |
| Psp124BI | GAGCTC | 1 cut(s) | 286 |
| Psp5II | RGGWCCY | 1 cut(s) | 1220 |
| PspN4I | GGNNCC | 3 cut(s) | 303, 1221, 1252 |
| PspPI | GGNCC | 1 cut(s) | 1220 |
| PspPPI | RGGWCCY | 1 cut(s) | 1220 |
| PspXI | VCTCGAGB | 1 cut(s) | 279 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 198, 230 |
| PstI | CTGCAG | 1 cut(s) | 580 |
| PvuII | CAGCTG | 1 cut(s) | 1342 |
| RsaI | GTAC | 3 cut(s) | 161, 207, 277 |
| RsaNI | GTAC | 3 cut(s) | 160, 206, 276 |
| RseI | CAYNNNNRTG | 2 cut(s) | 125, 228 |
| SacI | GAGCTC | 1 cut(s) | 286 |
| SaqAI | TTAA | 7 cut(s) | 467, 635, 644, 705, 774, 860, 1079 |
| SatI | GCNGC | 3 cut(s) | 607, 610, 1340 |
| Sau3AI | GATC | 6 cut(s) | 69, 667, 720, 822, 972, 1281 |
| Sau96I | GGNCC | 1 cut(s) | 1220 |
| SchI | GAGTC | 1 cut(s) | 922 |
| ScrFI | CCNGG | 1 cut(s) | 1314 |
| SduI | GDGCHC | 1 cut(s) | 286 |
| SfaNI | GCATC | 1 cut(s) | 699 |
| SfcI | CTRYAG | 3 cut(s) | 210, 576, 1286 |
| Sfr274I | CTCGAG | 1 cut(s) | 279 |
| SinI | GGWCC | 1 cut(s) | 1220 |
| SlaI | CTCGAG | 1 cut(s) | 279 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 125, 228 |
| SmlI | CTYRAG | 3 cut(s) | 279, 988, 1333 |
| SmoI | CTYRAG | 3 cut(s) | 279, 988, 1333 |
| Sse9I | AATT | 9 cut(s) | 256, 308, 381, 560, 698, 861, 911, 1115, 1228 |
| SseBI | AGGCCT | 1 cut(s) | 1239 |
| SsiI | CCGC | 2 cut(s) | 430, 801 |
| SspMI | CTAG | 6 cut(s) | 365, 495, 512, 591, 1094, 1224 |
| SstI | GAGCTC | 1 cut(s) | 286 |
| StuI | AGGCCT | 1 cut(s) | 1239 |
| StyD4I | CCNGG | 1 cut(s) | 1312 |
| StyI | CCWWGG | 2 cut(s) | 454, 1223 |
| TaaI | ACNGT | 2 cut(s) | 851, 1039 |
| TaiI | ACGT | 2 cut(s) | 362, 1017 |
| TaqI | TCGA | 4 cut(s) | 98, 280, 666, 723 |
| TaqII | GACCGA | 1 cut(s) | 1167 |
| TasI | AATT | 9 cut(s) | 256, 308, 381, 560, 698, 861, 911, 1115, 1228 |
| TatI | WGTACW | 1 cut(s) | 159 |
| TfiI | GAWTC | 5 cut(s) | 100, 478, 747, 983, 1155 |
| Tru1I | TTAA | 7 cut(s) | 467, 635, 644, 705, 774, 860, 1079 |
| Tru9I | TTAA | 7 cut(s) | 467, 635, 644, 705, 774, 860, 1079 |
| TscAI | CASTG | 3 cut(s) | 272, 1261, 1353 |
| TseFI | GTSAC | 1 cut(s) | 295 |
| TseI | GCWGC | 3 cut(s) | 606, 609, 1339 |
| Tsp45I | GTSAC | 1 cut(s) | 295 |
| TspDTI | ATGAA | 4 cut(s) | 218, 653, 996, 1192 |
| TspGWI | ACGGA | 1 cut(s) | 314 |
| TspRI | CASTG | 3 cut(s) | 272, 1261, 1353 |
| VpaK11BI | GGWCC | 1 cut(s) | 1220 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 884 |
| XapI | RAATTY | 3 cut(s) | 256, 560, 698 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 452 |
| XhoI | CTCGAG | 1 cut(s) | 279 |
| XmaJI | CCTAGG | 1 cut(s) | 1223 |
| XspI | CTAG | 6 cut(s) | 365, 495, 512, 591, 1094, 1224 |
| Zsp2I | ATGCAT | 2 cut(s) | 132, 235 |