cytochrome P450
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 644 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 1278, 1520 |
| AccII | CGCG | 1 cut(s) | 801 |
| AciI | CCGC | 1 cut(s) | 335 |
| AclI | AACGTT | 2 cut(s) | 962, 1296 |
| AclWI | GGATC | 3 cut(s) | 135, 1126, 1262 |
| AcoI | YGGCCR | 1 cut(s) | 477 |
| AcsI | RAATTY | 2 cut(s) | 119, 599 |
| AdeI | CACNNNGTG | 1 cut(s) | 1520 |
| AfaI | GTAC | 3 cut(s) | 313, 593, 888 |
| AfeI | AGCGCT | 1 cut(s) | 1451 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 213, 1179, 1180, 1278, 1520 |
| AgsI | TTSAA | 8 cut(s) | 42, 196, 302, 677, 709, 895, 1235, 1303 |
| AjiI | CACGTC | 2 cut(s) | 1243, 1291 |
| AjnI | CCWGG | 4 cut(s) | 132, 629, 1431, 1471 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 473, 505 |
| AluBI | AGCT | 9 cut(s) | 68, 192, 475, 504, 537, 651, 784, 1337, 1458 |
| AluI | AGCT | 9 cut(s) | 68, 192, 475, 504, 537, 651, 784, 1337, 1458 |
| Alw21I | GWGCWC | 1 cut(s) | 1242 |
| Alw26I | GTCTC | 4 cut(s) | 420, 536, 728, 846 |
| Alw44I | GTGCAC | 1 cut(s) | 1238 |
| AlwI | GGATC | 3 cut(s) | 135, 1126, 1262 |
| Aor51HI | AGCGCT | 1 cut(s) | 1451 |
| AoxI | GGCC | 5 cut(s) | 145, 215, 477, 1102, 1378 |
| ApaI | GGGCCC | 1 cut(s) | 1382 |
| ApaLI | GTGCAC | 1 cut(s) | 1238 |
| ApoI | RAATTY | 2 cut(s) | 119, 599 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1298 |
| AspLEI | GCGC | 1 cut(s) | 1452 |
| AspS9I | GGNCC | 8 cut(s) | 99, 146, 216, 273, 288, 1008, 1378, 1379 |
| AsuHPI | GGTGA | 4 cut(s) | 340, 461, 604, 1453 |
| AvaII | GGWCC | 4 cut(s) | 99, 273, 288, 1008 |
| BaeGI | GKGCMC | 2 cut(s) | 1242, 1382 |
| BalI | TGGCCA | 1 cut(s) | 479 |
| BanI | GGYRCC | 1 cut(s) | 644 |
| BanII | GRGCYC | 1 cut(s) | 1382 |
| BbsI | GAAGAC | 1 cut(s) | 1293 |
| Bbv12I | GWGCWC | 1 cut(s) | 1242 |
| BccI | CCATC | 3 cut(s) | 1019, 1094, 1207 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 565, 599, 1387, 1421 |
| BciT130I | CCWGG | 4 cut(s) | 134, 631, 1433, 1473 |
| BcoDI | GTCTC | 4 cut(s) | 420, 536, 728, 846 |
| BfaI | CTAG | 2 cut(s) | 534, 551 |
| BfmI | CTRYAG | 3 cut(s) | 246, 1445, 1453 |
| BfoI | RGCGCY | 1 cut(s) | 1453 |
| BmcAI | AGTACT | 1 cut(s) | 888 |
| Bme1390I | CCNGG | 4 cut(s) | 134, 631, 1433, 1473 |
| Bme18I | GGWCC | 4 cut(s) | 99, 273, 288, 1008 |
| BmgBI | CACGTC | 2 cut(s) | 1243, 1291 |
| BmgT120I | GGNCC | 8 cut(s) | 99, 146, 216, 273, 288, 1008, 1378, 1379 |
| BmiI | GGNNCC | 5 cut(s) | 101, 289, 339, 646, 1380 |
| BmrFI | CCNGG | 4 cut(s) | 134, 631, 1433, 1473 |
| BpiI | GAAGAC | 1 cut(s) | 1293 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 1301, 1333 |
| BpmI | CTGGAG | 3 cut(s) | 116, 870, 1146 |
| BpuEI | CTTGAG | 1 cut(s) | 806 |
| BsaAI | YACGTR | 1 cut(s) | 409 |
| BsaJI | CCNNGG | 7 cut(s) | 133, 166, 400, 438, 493, 1431, 1471 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1275, 1305, 1515, 1545 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 213, 1179, 1180, 1278, 1520 |
| Bse1I | ACTGG | 5 cut(s) | 26, 759, 853, 1163, 1529 |
| Bse3DI | GCAATG | 2 cut(s) | 98, 1443 |
| BseBI | CCWGG | 4 cut(s) | 134, 631, 1433, 1473 |
| BseDI | CCNNGG | 7 cut(s) | 133, 166, 400, 438, 493, 1431, 1471 |
| BseGI | GGATG | 1 cut(s) | 943 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 213, 1179, 1180, 1278, 1520 |
| BseMI | GCAATG | 2 cut(s) | 98, 1443 |
| BseMII | CTCAG | 6 cut(s) | 333, 335, 531, 630, 739, 1332 |
| BseNI | ACTGG | 5 cut(s) | 26, 759, 853, 1163, 1529 |
| BseSI | GKGCMC | 2 cut(s) | 1242, 1382 |
| BseYI | CCCAGC | 2 cut(s) | 682, 1204 |
| Bsh1236I | CGCG | 1 cut(s) | 801 |
| BshFI | GGCC | 5 cut(s) | 147, 217, 479, 1104, 1380 |
| BshNI | GGYRCC | 1 cut(s) | 644 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1242 |
| BsiSI | CCGG | 1 cut(s) | 1223 |
| BslFI | GGGAC | 3 cut(s) | 301, 382, 1229 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 213, 1179, 1180, 1278, 1520 |
| BsmAI | GTCTC | 4 cut(s) | 420, 536, 728, 846 |
| BsmBI | CGTCTC | 1 cut(s) | 846 |
| BsmFI | GGGAC | 3 cut(s) | 301, 382, 1229 |
| BsmI | GAATGC | 3 cut(s) | 27, 959, 1358 |
| BsnI | GGCC | 5 cut(s) | 147, 217, 479, 1104, 1380 |
| Bsp120I | GGGCCC | 1 cut(s) | 1378 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1242, 1382 |
| Bsp143I | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| Bsp19I | CCATGG | 3 cut(s) | 166, 438, 493 |
| BspACI | CCGC | 1 cut(s) | 335 |
| BspANI | GGCC | 5 cut(s) | 147, 217, 479, 1104, 1380 |
| BspCNI | CTCAG | 6 cut(s) | 334, 334, 532, 629, 738, 1331 |
| BspFNI | CGCG | 1 cut(s) | 801 |
| BspHI | TCATGA | 5 cut(s) | 186, 360, 568, 904, 1264 |
| BspLI | GGNNCC | 5 cut(s) | 101, 289, 339, 646, 1380 |
| BspMAI | CTGCAG | 1 cut(s) | 250 |
| BspPI | GGATC | 3 cut(s) | 135, 1126, 1262 |
| BspT107I | GGYRCC | 1 cut(s) | 644 |
| BsrDI | GCAATG | 2 cut(s) | 98, 1443 |
| BsrI | ACTGG | 5 cut(s) | 26, 759, 853, 1163, 1529 |
| BssECI | CCNNGG | 7 cut(s) | 133, 166, 400, 438, 493, 1431, 1471 |
| BssMI | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| BssT1I | CCWWGG | 4 cut(s) | 166, 400, 438, 493 |
| Bst2UI | CCWGG | 4 cut(s) | 134, 631, 1433, 1473 |
| Bst4CI | ACNGT | 2 cut(s) | 1507, 1548 |
| Bst6I | CTCTTC | 1 cut(s) | 386 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1427 |
| BstBAI | YACGTR | 1 cut(s) | 409 |
| BstC8I | GCNNGC | 4 cut(s) | 47, 215, 477, 1002 |
| BstDEI | CTNAG | 8 cut(s) | 321, 342, 540, 616, 655, 725, 1148, 1318 |
| BstDSI | CCRYGG | 3 cut(s) | 166, 438, 493 |
| BstF5I | GGATG | 1 cut(s) | 943 |
| BstFNI | CGCG | 1 cut(s) | 801 |
| BstH2I | RGCGCY | 1 cut(s) | 1453 |
| BstHHI | GCGC | 1 cut(s) | 1452 |
| BstKTI | GATC | 5 cut(s) | 130, 585, 771, 1134, 1270 |
| BstMAI | GTCTC | 4 cut(s) | 420, 536, 728, 846 |
| BstMBI | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 485, 1427 |
| BstNI | CCWGG | 4 cut(s) | 134, 631, 1433, 1473 |
| BstNSI | RCATGY | 1 cut(s) | 49 |
| BstSCI | CCNGG | 4 cut(s) | 132, 629, 1431, 1471 |
| BstSFI | CTRYAG | 3 cut(s) | 246, 1445, 1453 |
| BstSLI | GKGCMC | 2 cut(s) | 1242, 1382 |
| BstUI | CGCG | 1 cut(s) | 801 |
| BstV2I | GAAGAC | 1 cut(s) | 1293 |
| BstX2I | RGATCY | 1 cut(s) | 127 |
| BstYI | RGATCY | 1 cut(s) | 127 |
| BsuRI | GGCC | 5 cut(s) | 147, 217, 479, 1104, 1380 |
| BtgI | CCRYGG | 3 cut(s) | 166, 438, 493 |
| BtrI | CACGTC | 2 cut(s) | 1243, 1291 |
| BtsCI | GGATG | 1 cut(s) | 943 |
| BtsIMutI | CAGTG | 2 cut(s) | 766, 1544 |
| Cac8I | GCNNGC | 4 cut(s) | 47, 215, 477, 1002 |
| CciI | TCATGA | 5 cut(s) | 186, 360, 568, 904, 1264 |
| CfoI | GCGC | 1 cut(s) | 1452 |
| Cfr13I | GGNCC | 8 cut(s) | 99, 146, 216, 273, 288, 1008, 1378, 1379 |
| CseI | GACGC | 1 cut(s) | 863 |
| Csp6I | GTAC | 3 cut(s) | 312, 592, 887 |
| CviQI | GTAC | 3 cut(s) | 312, 592, 887 |
| DdeI | CTNAG | 8 cut(s) | 321, 342, 540, 616, 655, 725, 1148, 1318 |
| DpnI | GATC | 5 cut(s) | 129, 584, 770, 1133, 1269 |
| DpnII | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| DraIII | CACNNNGTG | 1 cut(s) | 1520 |
| EaeI | YGGCCR | 1 cut(s) | 477 |
| Eam1104I | CTCTTC | 1 cut(s) | 386 |
| EarI | CTCTTC | 1 cut(s) | 386 |
| Eco130I | CCWWGG | 4 cut(s) | 166, 400, 438, 493 |
| Eco24I | GRGCYC | 1 cut(s) | 1382 |
| Eco47I | GGWCC | 4 cut(s) | 99, 273, 288, 1008 |
| Eco47III | AGCGCT | 1 cut(s) | 1451 |
| EcoO109I | RGGNCCY | 2 cut(s) | 146, 1379 |
| EcoRII | CCWGG | 4 cut(s) | 132, 629, 1431, 1471 |
| EcoT14I | CCWWGG | 4 cut(s) | 166, 400, 438, 493 |
| EcoT38I | GRGCYC | 1 cut(s) | 1382 |
| ErhI | CCWWGG | 4 cut(s) | 166, 400, 438, 493 |
| Esp3I | CGTCTC | 1 cut(s) | 846 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1049, 1081 |
| FaqI | GGGAC | 3 cut(s) | 301, 382, 1229 |
| FokI | GGATG | 1 cut(s) | 950 |
| FriOI | GRGCYC | 1 cut(s) | 1382 |
| FspBI | CTAG | 2 cut(s) | 534, 551 |
| GlaI | GCGC | 1 cut(s) | 1451 |
| GsaI | CCCAGC | 2 cut(s) | 686, 1208 |
| GsuI | CTGGAG | 3 cut(s) | 116, 870, 1146 |
| HaeII | RGCGCY | 1 cut(s) | 1453 |
| HaeIII | GGCC | 5 cut(s) | 147, 217, 479, 1104, 1380 |
| HapII | CCGG | 1 cut(s) | 1223 |
| HgaI | GACGC | 1 cut(s) | 863 |
| HhaI | GCGC | 1 cut(s) | 1452 |
| Hin6I | GCGC | 1 cut(s) | 1450 |
| HinP1I | GCGC | 1 cut(s) | 1450 |
| HincII | GTYRAC | 1 cut(s) | 693 |
| HindII | GTYRAC | 1 cut(s) | 693 |
| HindIII | AAGCTT | 1 cut(s) | 782 |
| HinfI | GANTC | 6 cut(s) | 15, 138, 517, 1142, 1314, 1441 |
| HpaII | CCGG | 1 cut(s) | 1223 |
| HphI | GGTGA | 4 cut(s) | 340, 461, 604, 1453 |
| Hpy166II | GTNNAC | 7 cut(s) | 99, 592, 612, 693, 1240, 1294, 1483 |
| Hpy188I | TCNGA | 8 cut(s) | 14, 20, 386, 541, 619, 728, 778, 1321 |
| Hpy188III | TCNNGA | 8 cut(s) | 115, 187, 361, 521, 569, 905, 1135, 1265 |
| Hpy8I | GTNNAC | 7 cut(s) | 99, 592, 612, 693, 1240, 1294, 1483 |
| HpyAV | CCTTC | 6 cut(s) | 440, 499, 566, 706, 889, 1316 |
| HpyCH4III | ACNGT | 2 cut(s) | 1507, 1548 |
| HpyCH4IV | ACGT | 5 cut(s) | 408, 962, 1242, 1290, 1296 |
| HpyCH4V | TGCA | 4 cut(s) | 248, 957, 1025, 1240 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 485, 1427 |
| HpyF3I | CTNAG | 8 cut(s) | 321, 342, 540, 616, 655, 725, 1148, 1318 |
| HpySE526I | ACGT | 5 cut(s) | 408, 962, 1242, 1290, 1296 |
| HspAI | GCGC | 1 cut(s) | 1450 |
| Kzo9I | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| MaeI | CTAG | 2 cut(s) | 534, 551 |
| MaeII | ACGT | 5 cut(s) | 408, 962, 1242, 1290, 1296 |
| MalI | GATC | 5 cut(s) | 129, 584, 770, 1133, 1269 |
| MboI | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| MboII | GAAGA | 7 cut(s) | 290, 373, 424, 608, 676, 1010, 1298 |
| MflI | RGATCY | 1 cut(s) | 127 |
| MhlI | GDGCHC | 2 cut(s) | 1242, 1382 |
| MlsI | TGGCCA | 1 cut(s) | 479 |
| MluCI | AATT | 6 cut(s) | 119, 292, 599, 715, 991, 1070 |
| MluNI | TGGCCA | 1 cut(s) | 479 |
| MlyI | GAGTC | 1 cut(s) | 147 |
| MmeI | TCCRAC | 1 cut(s) | 756 |
| Mox20I | TGGCCA | 1 cut(s) | 479 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1298 |
| MscI | TGGCCA | 1 cut(s) | 479 |
| MseI | TTAA | 2 cut(s) | 467, 506 |
| MslI | CAYNNNNRTG | 1 cut(s) | 437 |
| Msp20I | TGGCCA | 1 cut(s) | 479 |
| MspI | CCGG | 1 cut(s) | 1223 |
| MspR9I | CCNGG | 4 cut(s) | 134, 631, 1433, 1473 |
| Mva1269I | GAATGC | 3 cut(s) | 27, 959, 1358 |
| MvaI | CCWGG | 4 cut(s) | 134, 631, 1433, 1473 |
| MvnI | CGCG | 1 cut(s) | 801 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 485, 1427 |
| NcoI | CCATGG | 3 cut(s) | 166, 438, 493 |
| NdeII | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| NlaIV | GGNNCC | 5 cut(s) | 101, 289, 339, 646, 1380 |
| NspI | RCATGY | 1 cut(s) | 49 |
| PaeI | GCATGC | 1 cut(s) | 49 |
| PagI | TCATGA | 5 cut(s) | 186, 360, 568, 904, 1264 |
| PcsI | WCGNNNNNNNCGW | 2 cut(s) | 591, 1516 |
| PctI | GAATGC | 3 cut(s) | 27, 959, 1358 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1298 |
| PfeI | GAWTC | 5 cut(s) | 15, 517, 1142, 1314, 1441 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 1278, 1520 |
| PfoI | TCCNGGA | 1 cut(s) | 629 |
| PleI | GAGTC | 1 cut(s) | 146 |
| PpsI | GAGTC | 1 cut(s) | 146 |
| Ppu21I | YACGTR | 1 cut(s) | 409 |
| Psp1406I | AACGTT | 2 cut(s) | 962, 1296 |
| Psp6I | CCWGG | 4 cut(s) | 132, 629, 1431, 1471 |
| PspFI | CCCAGC | 2 cut(s) | 682, 1204 |
| PspGI | CCWGG | 4 cut(s) | 132, 629, 1431, 1471 |
| PspN4I | GGNNCC | 5 cut(s) | 101, 289, 339, 646, 1380 |
| PspOMI | GGGCCC | 1 cut(s) | 1378 |
| PspPI | GGNCC | 8 cut(s) | 99, 146, 216, 273, 288, 1008, 1378, 1379 |
| PstI | CTGCAG | 1 cut(s) | 250 |
| PsuI | RGATCY | 1 cut(s) | 127 |
| RsaI | GTAC | 3 cut(s) | 313, 593, 888 |
| RsaNI | GTAC | 3 cut(s) | 312, 592, 887 |
| RseI | CAYNNNNRTG | 1 cut(s) | 437 |
| SaqAI | TTAA | 2 cut(s) | 467, 506 |
| Sau3AI | GATC | 5 cut(s) | 127, 582, 768, 1131, 1267 |
| Sau96I | GGNCC | 8 cut(s) | 99, 146, 216, 273, 288, 1008, 1378, 1379 |
| ScaI | AGTACT | 1 cut(s) | 888 |
| SchI | GAGTC | 1 cut(s) | 147 |
| ScrFI | CCNGG | 4 cut(s) | 134, 631, 1433, 1473 |
| SduI | GDGCHC | 2 cut(s) | 1242, 1382 |
| SfcI | CTRYAG | 3 cut(s) | 246, 1445, 1453 |
| SinI | GGWCC | 4 cut(s) | 99, 273, 288, 1008 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 437 |
| SmlI | CTYRAG | 1 cut(s) | 785 |
| SmoI | CTYRAG | 1 cut(s) | 785 |
| SphI | GCATGC | 1 cut(s) | 49 |
| Sse9I | AATT | 6 cut(s) | 119, 292, 599, 715, 991, 1070 |
| SsiI | CCGC | 1 cut(s) | 335 |
| SspMI | CTAG | 2 cut(s) | 534, 551 |
| StyD4I | CCNGG | 4 cut(s) | 132, 629, 1431, 1471 |
| StyI | CCWWGG | 4 cut(s) | 166, 400, 438, 493 |
| TaaI | ACNGT | 2 cut(s) | 1507, 1548 |
| TaiI | ACGT | 5 cut(s) | 411, 965, 1245, 1293, 1299 |
| TaqI | TCGA | 3 cut(s) | 585, 1029, 1384 |
| TasI | AATT | 6 cut(s) | 119, 292, 599, 715, 991, 1070 |
| TatI | WGTACW | 1 cut(s) | 886 |
| TfiI | GAWTC | 5 cut(s) | 15, 517, 1142, 1314, 1441 |
| Tru1I | TTAA | 2 cut(s) | 467, 506 |
| Tru9I | TTAA | 2 cut(s) | 467, 506 |
| TscAI | CASTG | 2 cut(s) | 766, 1551 |
| TspDTI | ATGAA | 9 cut(s) | 112, 377, 585, 930, 966, 1155, 1250, 1351, 1415 |
| TspGWI | ACGGA | 1 cut(s) | 1049 |
| TspRI | CASTG | 2 cut(s) | 766, 1551 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 1278, 1520 |
| VneI | GTGCAC | 1 cut(s) | 1238 |
| VpaK11BI | GGWCC | 4 cut(s) | 99, 273, 288, 1008 |
| XapI | RAATTY | 2 cut(s) | 119, 599 |
| XceI | RCATGY | 1 cut(s) | 49 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 491 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1298 |
| XspI | CTAG | 2 cut(s) | 534, 551 |
| ZrmI | AGTACT | 1 cut(s) | 888 |