Rw4G031310

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000078
Physical Location & Seq
Reverse (-)
58218741 .. 58250107
31367 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031310.1

Sequence Viewer

Length: 2769 bp
ATGAGTCGGGTGAGAGACACAGTCCTCACTCTTCCCGGTGTTTCGTGTGCCTTTCTTCTTCTGTTGGCTTTGATCTTCATCAAGATAGTTCACAAACTATGGTGGACTCCAACTCGAACACAGAGGTTCATGGCTTCTCAGGGAATCAGAGGTCCTTCTTACAGACTCATCCATGGAAACAACAAAGAAATCAGCAACATGCTAAAGGAAGCCATGAGCAGGCCCATACCGAATTTATCACATGATGTACTATCTGCAGTTCAACCTCACATTCACTCATGGGCCAAGAGCTATGGGAAGAATTATCTACAATGGTGTGGTCTTCAACCAGTTTTAGTCATTACAGAACCTGAGTTAGGCAAGGAGATTCTCAATAACAAAGATAGGGTTTATACAAAACAGAAGCCCAGTATCTATGTGAAGAAGCTATTGGGAGACAGCGTTTCCATGGCAGAAGGTGAAAAATGGTCAAAACTGAGAAAGATTTCCAACCATGCCTTCCATGGAGACAGCTTAAAAAGTATGATTCCAGACATGATAGCTAGTGCTGAGATGATGCTCGAAGGATGGAAAAAGCATGAAGGAAAAGAGATTGAGGTGTATGAACATTTTAGGTTGTTCACTTCAGAAGTGATTTCTAGGACAGCATTTGGCAGCAGCTATTTAGAAGGGAAGAATATTTTTGACATGTTGATGAAGTTAAGCTCTGTAATATTCAAAAATGCTCACAAACTCAGGGTTCCTGGCATCAGCAAGTTTTATAAAACCAGTGATGAGAAAGAATCAGAGAAGCTTGAGAAAGGAATAGGAGACACCATAATAGAGATTGTTAAGAAAAGAGAGAGGGAGGCAGTGACTGGAGAAGAAGGCAGCTTTGGGACTGATTTTCTTGGATTGCTTTTAAAGGCTCGCCATGCTACCAATGAAAAGCAGAGGATTTCAGTGAATGAATTGGTCGAGGAGTGCAAGACATTTTACTTTGCTGGACAAGAAACGACTAACTCTTTGCTTGTTTGGACTGTCTTTCTTCTGGCCCTCCATCCGGAATGGCAAGAGGAAGCAAGAAAGGAGGTCCTGCAGTTATTTGGCAAAAAAACTCCAAATCCTGATGGCCTTGCCAAACTAAAAACGATGAGTATGATCATCAATGAGTCTCTGAGGCTATATCCTCCTGTCATTTTCCTTGTACGAACAGTGGATAAGGAAGTTAAATTGGGAAACCTCATTATACCTGCTAATGTTGAATTGCTTGTCTCAAATCTAGCACTTCATCATGAACCTCAGTTCTGGGGACAAGATGTGAAACATTTCAAACCTGAGAGATTCTCTGAAGGTGTTGCTAGTGCTACTAACAACAACATGGCTGCATTCTTACCCTTTGGAATGGGACCTAGAACTTGCGTTGGCCTCAACTTTGCCACCATTGAAGCAAAGATTGCTCTTTCAATGATTCTACAACGCTACTCCTTCACCCTTTCCCCAGCCTATGTCCACTCGCCCTTTCAGTTTCTCACAGTTCGTCCACAGCATGGACTTCAAGTAATTTTATACTCGGTCTTTTATGAACTATGGTGGACTCCGATTCGCATTCAGAATTTGATGAGTCTGCAGGGAATCAAAGGTCCTCCTTACAGACTTCTCTATGGAAACGTCAAAGAAATCACCAACATGCAGAAGGAAGCCATGAGCAGACCCATAAGGAAGAATTTTCTTCAGTGGTATGGTCTTCAACCAGTTTTGGTTGTTACAAAACCTGACTTGTGCAAAGAGATCCTCAATAACAAAGACGAAGTTTATCCAAAACCGAAGCCTAGTGTCTTTGTGAATAAGCTATTTAGAGATAGCCTTTCCATGACAGAAGGAAAAAAATGGGCCAAGTTGAGAAAGCTGTCCAACCTTGTCTTCCATGGAGAGAGCTTAAAAGCTATGTTTCCAGAAATGATAGCTAGTGCCGAGGCGATGGTAGAAAGGTGGAAAAATCATGAAGGAAAAGAGATTGATGTGTTTGAAGAATTTAGATTGTTGACTTCAGAAGTGATTTCCAGGACAGCTTTTGGCAGCAGCTACTTAGAAGGACAAAACATTTTAGAAATGTTGATGAAGTTAAGCGTCATAGCTCATCATGACACCAATGACAACCAGAGGATTTCTATAGACGATTTGGTTGAAGAATGCAAGTCGTTATACTTTGTTGGACAAGAGACAACTAATACTTTGCTTTCTTGGACTGTATTTCTTCTGGCACAACATCCTGCTTGGCAAGAAGAAGCGAGAAAGGAGGTGCTGCAGTTATTTGGCAAACAAACTCCCAATTCTGATGGCACTTCCAAACTCAAAACAATGAGCATGATCATTAATGAGACTCTAAGGTTATATCCTCCTGCTAATTTTCTTGTAAGAGATGTTGCAAGGAGTGTTCGACTGGGAAAAATCCTAGTTCCTGCAAATATTGAATTGGTTATCCCAACTCTTCCACTTCACCATGATCCTCAATTATGGGGACAACACATGGAACTTTTCAAACCAGAACAATTCTCTGAAGGCATTGCTAAAGCTAGTAATGATAACATGGCTTCATTCTTACCCTTTGGAATGGGACCTAGAACGTGTGTGGGATTCAACTTTGCCTCCAATGAAGCAAAGATTGCTCTATCAATGATTCTACAACACTACTCATTTACCCTCTCCCTGGGTTATGTCCACTCACCTTTTCAGTTTCTTGCGCTTCGTCCAAAATATGGAGTTCAAGTAATACTACACTCTCTGTGA

Protein Analysis

922

Amino Acids

105.18

Weight (kDa)

9.15

Isoelectric Point (pI)

37.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 91 - 495 2.3e-81 Cytochrome P450
p450 PF00067 541 - 699 4.9e-15 Cytochrome P450
p450 PF00067 699 - 906 1.7e-46 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000078)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g15240 FvH4_3g15270 FvH4_3g15280 FvH4_3g32750 FvH4_3g32760 FvH4_3g32760 FvH4_3g32880 FvH4_4g08190 FvH4_4g29160 FvH4_4g29370 FvH4_4g29400 FvH4_4g29420 FvH4_4g29420 FvH4_4g29420 FvH4_4g29430 FvH4_4g29440 FvH4_4g29450 FvH4_4g29450 FvH4_4g29450 FvH4_4g29460 FvH4_4g29461 FvH4_4g29462 FvH4_4g29463 FvH4_4g29480 FvH4_4g29481 FvH4_4g29482 FvH4_4g29483 FvH4_4g29484 FvH4_6g26720 FvH4_6g26720
malus_domestica MD00G1065200.v1.1 MD06G1145800.v1.1 MD06G1146000.v1.1 MD06G1146700.v1.1 MD13G1031000.v1.1 MD13G1033900.v1.1 MD13G1057000.v1.1 MD13G1057100.v1.1 MD13G1057200.v1.1 MD13G1057400.v1.1 MD13G1057800.v1.1 MD13G1058000.v1.1 MD13G1058100.v1.1 MD13G1058200.v1.1 MD13G1058300.v1.1 MD13G1058500.v1.1 MD13G1058600.v1.1 MD13G1058700.v1.1 MD16G1035700.v1.1 MD16G1056400.v1.1 MD16G1056600.v1.1 MD16G1056700.v1.1 MD16G1056900.v1.1 MD16G1058100.v1.1
prunus_persica Prupe.1G291100_v2.0.a1 Prupe.1G291200_v2.0.a1 Prupe.1G291500_v2.0.a1 Prupe.1G291600_v2.0.a1 Prupe.1G291700_v2.0.a1 Prupe.1G291800_v2.0.a1 Prupe.1G291900_v2.0.a1 Prupe.1G292000_v2.0.a1 Prupe.1G292100_v2.0.a1 Prupe.1G292400_v2.0.a1 Prupe.1G292500_v2.0.a1 Prupe.1G292600_v2.0.a1 Prupe.1G292700_v2.0.a1 Prupe.1G292800_v2.0.a1 Prupe.1G296000_v2.0.a1 Prupe.1G296100_v2.0.a1 Prupe.1G296300_v2.0.a1 Prupe.1G296600_v2.0.a1 Prupe.1G296900_v2.0.a1 Prupe.1G534600_v2.0.a1 Prupe.5G046700_v2.0.a1 Prupe.8G021800_v2.0.a1 Prupe.8G026400_v2.0.a1
pyrus_communis pycom06g13510 pycom06g13530 pycom06g13540 pycom06g13570 pycom06g13590 pycom06g13620 pycom13g02710 pycom13g02930 pycom13g05100 pycom13g05110 pycom13g05140 pycom13g05160 pycom13g05170 pycom13g05190 pycom13g05200 pycom13g05210 pycom13g05220 pycom16g03060 pycom16g04940 pycom16g04960 pycom16g04990 pycom16g05000 pycom16g05080
rosa_chinensis RchiOBHm_Chr1g0337981 RchiOBHm_Chr1g0337991 RchiOBHm_Chr1g0339431 RchiOBHm_Chr4g0402401 RchiOBHm_Chr4g0420571 RchiOBHm_Chr4g0420601 RchiOBHm_Chr4g0420611 RchiOBHm_Chr4g0420621 RchiOBHm_Chr4g0420631 RchiOBHm_Chr4g0437211 RchiOBHm_Chr4g0437221 RchiOBHm_Chr4g0437251 RchiOBHm_Chr4g0437431 RchiOBHm_Chr4g0437441 RchiOBHm_Chr4g0437491 RchiOBHm_Chr4g0437501 RchiOBHm_Chr4g0437511 RchiOBHm_Chr4g0437521 RchiOBHm_Chr4g0437531 RchiOBHm_Chr4g0437541 RchiOBHm_Chr4g0437551 RchiOBHm_Chr4g0437591 RchiOBHm_Chr4g0437631 RchiOBHm_Chr4g0437641 RchiOBHm_Chr4g0437671 RchiOBHm_Chr4g0437681 RchiOBHm_Chr4g0437711 RchiOBHm_Chr4g0437731 RchiOBHm_Chr4g0437741 RchiOBHm_Chr4g0437751 RchiOBHm_Chr5g0025681 RchiOBHm_Chr5g0025711 RchiOBHm_Chr5g0025731 RchiOBHm_Chr5g0059841 RchiOBHm_Chr6g0272651
rosa_laevigata RLG00000006420 RLG00000006421 RLG00000006423 RLG00000006426 RLG00000006427 RLG00000006428 RLG00000006429 RLG00000006431 RLG00000006433 RLG00000006434 RLG00000006435 RLG00000006436 RLG00000006437 RLG00000006455 RLG00000007746 RLG00000007747 RLG00000007748 RLG00000007749 RLG00000007750 RLG00000009091 RLG00000009092 RLG00000009095 RLG00000009096 RLG00000017856 RLG00000028094 RLG00000029230 RLG00000029231 RLG00000030008 RLG00000032859 RLG00000032860 RLG00000032861 RLG00000032865 RLG00000032868 RLG00000035332
rosa_multiflora Rmu_co7986016.1_g000001 Rmu_co8226539.1_g000001 Rmu_co8349613.1_g000001 Rmu_sc0000272.1_g000014 Rmu_sc0000591.1_g000003 Rmu_sc0000613.1_g000013 Rmu_sc0001629.1_g000022 Rmu_sc0001629.1_g000025 Rmu_sc0002131.1_g000027 Rmu_sc0002137.1_g000006 Rmu_sc0002137.1_g000014 Rmu_sc0002137.1_g000019 Rmu_sc0002472.1_g000002 Rmu_sc0002472.1_g000012 Rmu_sc0002472.1_g000018 Rmu_sc0002472.1_g000034 Rmu_sc0002472.1_g000040 Rmu_sc0002472.1_g000051 Rmu_sc0002638.1_g000005 Rmu_sc0002638.1_g000010 Rmu_sc0002638.1_g000025 Rmu_sc0002638.1_g000030 Rmu_sc0002638.1_g000037 Rmu_sc0002638.1_g000042 Rmu_sc0004339.1_g000019 Rmu_sc0005580.1_g000013 Rmu_sc0006617.1_g000002 Rmu_sc0006986.1_g000003 Rmu_sc0006986.1_g000017 Rmu_sc0008352.1_g000001 Rmu_sc0008352.1_g000002 Rmu_sc0009431.1_g000011 Rmu_sc0011080.1_g000003 Rmu_sc0011080.1_g000006 Rmu_sc0016549.1_g000001 Rmu_sc0016549.1_g000002 Rmu_sc0017154.1_g000001 Rmu_sc0017155.1_g000001 Rmu_sc0028438.1_g000001 Rmu_sc0041413.1_g000001 Rmu_ssc0000330.1_g000022
rosa_roxburghii Rroxscaffold_1G00020750 Rroxscaffold_1G00053460 Rroxscaffold_1G00054250 Rroxscaffold_1G00054260 Rroxscaffold_1G00054270 Rroxscaffold_1G00054290 Rroxscaffold_2G00120210 Rroxscaffold_4G00313460 Rroxscaffold_4G00313470 Rroxscaffold_4G00330630 Rroxscaffold_5G00347070 Rroxscaffold_5G00347110 Rroxscaffold_5G00378670 Rroxscaffold_5G00378690 Rroxscaffold_5G00378720 Rroxscaffold_5G00378730 Rroxscaffold_5G00378740 Rroxscaffold_5G00378760 Rroxscaffold_5G00378780 Rroxscaffold_5G00378790 Rroxscaffold_5G00378800 Rroxscaffold_5G00378810 Rroxscaffold_5G00378820 Rroxscaffold_5G00378840 Rroxscaffold_5G00378860 Rroxscaffold_5G00378870 Rroxscaffold_7G00196060
rosa_rugosa Rorug01G0142200.1 Rorug01G0332800 Rorug02G0012200 Rorug02G0248300 Rorug03G0219000 Rorug03G0219100 Rorug03G0219200 Rorug04G0035100 Rorug04G0301500 Rorug04G0301500 Rorug04G0301700 Rorug04G0301800 Rorug04G0301800 Rorug04G0301900 Rorug04G0302000 Rorug04G0302500 Rorug04G0302600 Rorug04G0302700 Rorug04G0302800 Rorug04G0302900 Rorug04G0303000 Rorug04G0303000 Rorug04G0303100 Rorug04G0303200 Rorug04G0303300 Rorug04G0303400 Rorug04G0303500 Rorug04G0303500 Rorug05G0087800 Rorug05G0088200 Rorug05G0326300 Rorug06G0173000
rosa_samantha Rh2AG222400 Rh4DG224800 Rh4DG359900 Rh4DG360000 Rh4DG360100 Rh4DG360200 Rh4DG360300 Rh4DG360500 Rh4DG360700 Rh4DG360800 Rh4DG360900 Rh4DG361000 Rh5DG177900 Rh5DG178200 Rh5DG178400 Rh6CG187800
rosa_wichuraiana Rw1G013580 Rw4G009020 Rw4G019580 Rw4G030990 Rw4G031000 Rw4G031220 Rw4G031230 Rw4G031240 Rw4G031250 Rw4G031260 Rw4G031270 Rw4G031280 Rw4G031290 Rw4G031310 Rw4G031320 Rw4G031330 Rw4G031340 Rw4G031360 Rw4G031370 Rw5G016310 Rw5G016320 Rw5G016330 Rw5G036760 Rw6G016090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 762
Acc36I ACCTGC 1 cut(s) 1240
AccB7I CCANNNNNTGG 2 cut(s) 1529, 2738
AccIII TCCGGA 1 cut(s) 1042
AclWI GGATC 2 cut(s) 1765, 2480
AcsI RAATTY 4 cut(s) 232, 1594, 1705, 2012
AcuI CTGAAG 5 cut(s) 609, 1350, 1697, 2013, 2559
AfaI GTAC 2 cut(s) 249, 1188
AfiI CCNNNNNNNGG 6 cut(s) 219, 356, 1042, 1529, 2689, 2738
AflIII ACRYGT 2 cut(s) 687, 2606
AjnI CCWGG 3 cut(s) 742, 2042, 2688
AjuI GAANNNNNNNTTGG 8 cut(s) 413, 445, 482, 514, 858, 890, 1886, 1918
Alw26I GTCTC 8 cut(s) 9, 429, 501, 804, 1158, 1258, 2195, 2354
AlwI GGATC 2 cut(s) 1765, 2480
AlwNI CAGNNNCTG 2 cut(s) 350, 857
Aor13HI TCCGGA 1 cut(s) 1042
AoxI GGCC 6 cut(s) 221, 282, 1032, 1111, 1405, 1872
ApeKI GCWGC 7 cut(s) 654, 657, 870, 1364, 2058, 2061, 2284
ApoI RAATTY 4 cut(s) 232, 1594, 1705, 2012
ArsI GACNNNNNNTTYG 2 cut(s) 988, 1020
AseI ATTAAT 1 cut(s) 2355
Asp700I GAANNNNTTC 2 cut(s) 484, 1307
AspLEI GCGC 1 cut(s) 2725
AspS9I GGNCC 9 cut(s) 152, 222, 282, 1033, 1072, 1388, 1622, 1872, 2597
AsuC2I CCSGG 1 cut(s) 36
AsuHPI GGTGA 6 cut(s) 22, 470, 1462, 1654, 2471, 2697
AvaII GGWCC 5 cut(s) 152, 1072, 1388, 1622, 2597
BbsI GAAGAC 3 cut(s) 314, 1718, 1894
BbvI GCAGC 7 cut(s) 666, 669, 882, 1351, 2070, 2073, 2271
BccI CCATC 5 cut(s) 561, 1047, 1103, 1954, 2312
BciT130I CCWGG 3 cut(s) 744, 2044, 2690
BclI TGATCA 2 cut(s) 1140, 2349
BcnI CCSGG 1 cut(s) 36
BcoDI GTCTC 8 cut(s) 9, 429, 501, 804, 1158, 1258, 2195, 2354
BfmI CTRYAG 5 cut(s) 255, 1076, 1607, 2151, 2285
BfuAI ACCTGC 1 cut(s) 1240
BisI GCNGC 7 cut(s) 655, 658, 871, 1365, 2059, 2062, 2285
BlsI GCNGC 7 cut(s) 656, 659, 872, 1366, 2060, 2063, 2286
Bme1390I CCNGG 4 cut(s) 36, 744, 2044, 2690
Bme18I GGWCC 5 cut(s) 152, 1072, 1388, 1622, 2597
BmgT120I GGNCC 9 cut(s) 152, 222, 282, 1033, 1072, 1388, 1622, 1872, 2597
BmiI GGNNCC 3 cut(s) 741, 1389, 2598
BmrFI CCNGG 4 cut(s) 36, 744, 2044, 2690
BmrI ACTGGG 2 cut(s) 402, 2432
BmsI GCATC 2 cut(s) 546, 756
BmuI ACTGGG 2 cut(s) 402, 2432
BpiI GAAGAC 3 cut(s) 314, 1718, 1894
BplI GAGNNNNNCTC 2 cut(s) 1310, 1342
BpmI CTGGAG 1 cut(s) 879
BpuEI CTTGAG 1 cut(s) 815
BpuMI CCSGG 1 cut(s) 36
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 7 cut(s) 172, 447, 502, 1906, 1953, 2688, 2689
BsaWI WCCGGW 1 cut(s) 1042
BsaXI ACNNNNNCTCC 6 cut(s) 426, 456, 2612, 2642, 2733, 2763
Bsc4I CCNNNNNNNGG 6 cut(s) 219, 356, 1042, 1529, 2689, 2738
Bse1I ACTGG 6 cut(s) 329, 408, 768, 862, 1733, 2427
Bse3DI GCAATG 1 cut(s) 2544
Bse8I GATNNNNATC 1 cut(s) 77
BseAI TCCGGA 1 cut(s) 1042
BseBI CCWGG 3 cut(s) 744, 2044, 2690
BseDI CCNNGG 7 cut(s) 172, 447, 502, 1906, 1953, 2688, 2689
BseGI GGATG 4 cut(s) 168, 572, 1039, 2248
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 6 cut(s) 219, 356, 1042, 1529, 2689, 2738
BseMI GCAATG 1 cut(s) 2544
BseMII CTCAG 8 cut(s) 152, 342, 467, 540, 748, 1148, 1295, 1308
BseNI ACTGG 6 cut(s) 329, 408, 768, 862, 1733, 2427
BseRI GAGGAG 1 cut(s) 974
BseXI GCAGC 7 cut(s) 666, 669, 882, 1351, 2070, 2073, 2271
BseYI CCCAGC 1 cut(s) 1480
BshFI GGCC 6 cut(s) 223, 284, 1034, 1113, 1407, 1874
BsiSI CCGG 2 cut(s) 36, 1043
BslFI GGGAC 5 cut(s) 892, 1305, 1401, 2514, 2610
BslI CCNNNNNNNGG 6 cut(s) 219, 356, 1042, 1529, 2689, 2738
BsmAI GTCTC 8 cut(s) 9, 429, 501, 804, 1158, 1258, 2195, 2354
BsmFI GGGAC 5 cut(s) 892, 1305, 1401, 2514, 2610
BsmI GAATGC 3 cut(s) 1367, 1587, 2177
BsnI GGCC 6 cut(s) 223, 284, 1034, 1113, 1407, 1874
Bsp13I TCCGGA 1 cut(s) 1042
Bsp143I GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
Bsp19I CCATGG 4 cut(s) 172, 447, 502, 1906
BspANI GGCC 6 cut(s) 223, 284, 1034, 1113, 1407, 1874
BspCNI CTCAG 8 cut(s) 151, 343, 468, 541, 747, 1149, 1294, 1309
BspEI TCCGGA 1 cut(s) 1042
BspHI TCATGA 3 cut(s) 1273, 1981, 2122
BspLI GGNNCC 3 cut(s) 741, 1389, 2598
BspMAI CTGCAG 4 cut(s) 259, 1080, 1611, 2289
BspMI ACCTGC 1 cut(s) 1240
BspPI GGATC 2 cut(s) 1765, 2480
BsrDI GCAATG 1 cut(s) 2544
BsrI ACTGG 6 cut(s) 329, 408, 768, 862, 1733, 2427
BssECI CCNNGG 7 cut(s) 172, 447, 502, 1906, 1953, 2688, 2689
BssMI GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
BssT1I CCWWGG 4 cut(s) 172, 447, 502, 1906
Bst2UI CCWGG 3 cut(s) 744, 2044, 2690
Bst4CI ACNGT 5 cut(s) 22, 1021, 1195, 1516, 2230
Bst6I CTCTTC 2 cut(s) 36, 2475
BstAPI GCANNNNNTGC 2 cut(s) 1436, 2645
BstC8I GCNNGC 2 cut(s) 221, 910
BstDSI CCRYGG 4 cut(s) 172, 447, 502, 1906
BstENI CCTNNNNNAGG 1 cut(s) 354
BstF5I GGATG 4 cut(s) 168, 572, 1039, 2248
BstHHI GCGC 1 cut(s) 2725
BstKTI GATC 5 cut(s) 75, 1143, 1773, 2352, 2488
BstMAI GTCTC 8 cut(s) 9, 429, 501, 804, 1158, 1258, 2195, 2354
BstMBI GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
BstMWI GCNNNNNNNGC 3 cut(s) 914, 1436, 2645
BstNI CCWGG 3 cut(s) 744, 2044, 2690
BstNSI RCATGY 3 cut(s) 202, 691, 1672
BstSCI CCNGG 4 cut(s) 34, 742, 2042, 2688
BstSFI CTRYAG 5 cut(s) 255, 1076, 1607, 2151, 2285
BstV1I GCAGC 7 cut(s) 666, 669, 882, 1351, 2070, 2073, 2271
BstV2I GAAGAC 3 cut(s) 314, 1718, 1894
BstX2I RGATCY 1 cut(s) 1770
BstYI RGATCY 1 cut(s) 1770
BsuRI GGCC 6 cut(s) 223, 284, 1034, 1113, 1407, 1874
BtgI CCRYGG 4 cut(s) 172, 447, 502, 1906
BtgZI GCGATG 1 cut(s) 1973
BtsCI GGATG 4 cut(s) 168, 572, 1039, 2248
BtsI GCAGTG 1 cut(s) 858
BtsIMutI CAGTG 5 cut(s) 775, 858, 948, 1200, 1721
BveI ACCTGC 1 cut(s) 1240
Cac8I GCNNGC 2 cut(s) 221, 910
CaiI CAGNNNCTG 2 cut(s) 350, 857
CciI TCATGA 3 cut(s) 1273, 1981, 2122
CfoI GCGC 1 cut(s) 2725
Cfr13I GGNCC 9 cut(s) 152, 222, 282, 1033, 1072, 1388, 1622, 1872, 2597
CseI GACGC 1 cut(s) 2098
Csp6I GTAC 2 cut(s) 248, 1187
CviQI GTAC 2 cut(s) 248, 1187
DpnI GATC 5 cut(s) 74, 1142, 1772, 2351, 2487
DpnII GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
DraI TTTAAA 1 cut(s) 903
Eam1104I CTCTTC 2 cut(s) 36, 2475
EarI CTCTTC 2 cut(s) 36, 2475
Eco130I CCWWGG 4 cut(s) 172, 447, 502, 1906
Eco47I GGWCC 5 cut(s) 152, 1072, 1388, 1622, 2597
Eco57I CTGAAG 5 cut(s) 609, 1350, 1697, 2013, 2559
EcoNI CCTNNNNNAGG 1 cut(s) 354
EcoO109I RGGNCCY 5 cut(s) 152, 1072, 1388, 1622, 2597
EcoRII CCWGG 3 cut(s) 742, 2042, 2688
EcoT14I CCWWGG 4 cut(s) 172, 447, 502, 1906
ErhI CCWWGG 4 cut(s) 172, 447, 502, 1906
FaqI GGGAC 5 cut(s) 892, 1305, 1401, 2514, 2610
FbaI TGATCA 2 cut(s) 1140, 2349
Fnu4HI GCNGC 7 cut(s) 655, 658, 871, 1365, 2059, 2062, 2285
FokI GGATG 4 cut(s) 155, 579, 1026, 2235
Fsp4HI GCNGC 7 cut(s) 655, 658, 871, 1365, 2059, 2062, 2285
GlaI GCGC 1 cut(s) 2724
GluI GCNGC 7 cut(s) 655, 658, 871, 1365, 2059, 2062, 2285
GsaI CCCAGC 1 cut(s) 1484
GsuI CTGGAG 1 cut(s) 879
HaeIII GGCC 6 cut(s) 223, 284, 1034, 1113, 1407, 1874
HapII CCGG 2 cut(s) 36, 1043
HgaI GACGC 1 cut(s) 2098
HhaI GCGC 1 cut(s) 2725
Hin6I GCGC 1 cut(s) 2723
HinP1I GCGC 1 cut(s) 2723
HincII GTYRAC 1 cut(s) 2025
HindII GTYRAC 1 cut(s) 2025
HindIII AAGCTT 1 cut(s) 791
HpaII CCGG 2 cut(s) 36, 1043
HphI GGTGA 6 cut(s) 22, 470, 1462, 1654, 2471, 2697
Hpy166II GTNNAC 8 cut(s) 91, 105, 621, 1492, 1523, 1575, 2025, 2701
Hpy188III TCNNGA 8 cut(s) 82, 530, 1043, 1106, 1274, 1934, 1982, 2123
Hpy8I GTNNAC 8 cut(s) 91, 105, 621, 1492, 1523, 1575, 2025, 2701
HpyCH4III ACNGT 5 cut(s) 22, 1021, 1195, 1516, 2230
HpyCH4IV ACGT 2 cut(s) 1650, 2606
HpyF10VI GCNNNNNNNGC 3 cut(s) 914, 1436, 2645
HpySE526I ACGT 2 cut(s) 1650, 2606
HspAI GCGC 1 cut(s) 2723
Kpn2I TCCGGA 1 cut(s) 1042
Ksp22I TGATCA 2 cut(s) 1140, 2349
Kzo9I GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
Lsp1109I GCAGC 7 cut(s) 666, 669, 882, 1351, 2070, 2073, 2271
LweI GCATC 2 cut(s) 546, 756
MaeII ACGT 2 cut(s) 1650, 2606
MaeIII GTNAC 2 cut(s) 853, 1744
MalI GATC 5 cut(s) 74, 1142, 1772, 2351, 2487
MboI GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
MflI RGATCY 1 cut(s) 1770
MlyI GAGTC 7 cut(s) 13, 100, 159, 1160, 1570, 1612, 2356
MmeI TCCRAC 4 cut(s) 134, 513, 1917, 2173
MroI TCCGGA 1 cut(s) 1042
MroXI GAANNNNTTC 2 cut(s) 484, 1307
MseI TTAA 8 cut(s) 515, 701, 831, 902, 1209, 1919, 2105, 2355
MslI CAYNNNNRTG 2 cut(s) 692, 1667
MspI CCGG 2 cut(s) 36, 1043
MspR9I CCNGG 4 cut(s) 36, 744, 2044, 2690
Mva1269I GAATGC 3 cut(s) 1367, 1587, 2177
MvaI CCWGG 3 cut(s) 744, 2044, 2690
MwoI GCNNNNNNNGC 3 cut(s) 914, 1436, 2645
NciI CCSGG 1 cut(s) 36
NcoI CCATGG 4 cut(s) 172, 447, 502, 1906
NdeII GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
NlaIV GGNNCC 3 cut(s) 741, 1389, 2598
NmeAIII GCCGAG 1 cut(s) 1978
NmuCI GTSAC 1 cut(s) 853
NspI RCATGY 3 cut(s) 202, 691, 1672
PagI TCATGA 3 cut(s) 1273, 1981, 2122
PasI CCCWGGG 1 cut(s) 2689
PciI ACATGT 1 cut(s) 687
PctI GAATGC 3 cut(s) 1367, 1587, 2177
PdmI GAANNNNTTC 2 cut(s) 484, 1307
PflFI GACNNNGTC 1 cut(s) 20
PflMI CCANNNNNTGG 2 cut(s) 1529, 2738
PfoI TCCNGGA 1 cut(s) 2042
PkrI GCNGC 7 cut(s) 656, 659, 872, 1366, 2060, 2063, 2286
PleI GAGTC 7 cut(s) 12, 100, 159, 1159, 1570, 1611, 2356
PpsI GAGTC 7 cut(s) 12, 100, 159, 1159, 1570, 1611, 2356
PpuMI RGGWCCY 5 cut(s) 152, 1072, 1388, 1622, 2597
PscI ACATGT 1 cut(s) 687
PshBI ATTAAT 1 cut(s) 2355
PsiI TTATAA 1 cut(s) 762
Psp5II RGGWCCY 5 cut(s) 152, 1072, 1388, 1622, 2597
Psp6I CCWGG 3 cut(s) 742, 2042, 2688
PspFI CCCAGC 1 cut(s) 1480
PspGI CCWGG 3 cut(s) 742, 2042, 2688
PspN4I GGNNCC 3 cut(s) 741, 1389, 2598
PspPI GGNCC 9 cut(s) 152, 222, 282, 1033, 1072, 1388, 1622, 1872, 2597
PspPPI RGGWCCY 5 cut(s) 152, 1072, 1388, 1622, 2597
PstI CTGCAG 4 cut(s) 259, 1080, 1611, 2289
PstNI CAGNNNCTG 2 cut(s) 350, 857
PsuI RGATCY 1 cut(s) 1770
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 2 cut(s) 249, 1188
RsaNI GTAC 2 cut(s) 248, 1187
RseI CAYNNNNRTG 2 cut(s) 692, 1667
SaqAI TTAA 8 cut(s) 515, 701, 831, 902, 1209, 1919, 2105, 2355
SatI GCNGC 7 cut(s) 655, 658, 871, 1365, 2059, 2062, 2285
Sau3AI GATC 5 cut(s) 72, 1140, 1770, 2349, 2485
Sau96I GGNCC 9 cut(s) 152, 222, 282, 1033, 1072, 1388, 1622, 1872, 2597
SchI GAGTC 7 cut(s) 13, 100, 159, 1160, 1570, 1612, 2356
ScrFI CCNGG 4 cut(s) 36, 744, 2044, 2690
SfaNI GCATC 2 cut(s) 546, 756
SfcI CTRYAG 5 cut(s) 255, 1076, 1607, 2151, 2285
SinI GGWCC 5 cut(s) 152, 1072, 1388, 1622, 2597
SmiMI CAYNNNNRTG 2 cut(s) 692, 1667
SmlI CTYRAG 1 cut(s) 794
SmoI CTYRAG 1 cut(s) 794
SspI AATATT 3 cut(s) 679, 714, 2449
StyD4I CCNGG 4 cut(s) 34, 742, 2042, 2688
StyI CCWWGG 4 cut(s) 172, 447, 502, 1906
TaaI ACNGT 5 cut(s) 22, 1021, 1195, 1516, 2230
TaiI ACGT 2 cut(s) 1653, 2609
TaqI TCGA 4 cut(s) 115, 561, 957, 2419
TaqII GACCGA 1 cut(s) 1543
TatI WGTACW 1 cut(s) 247
Tru1I TTAA 8 cut(s) 515, 701, 831, 902, 1209, 1919, 2105, 2355
Tru9I TTAA 8 cut(s) 515, 701, 831, 902, 1209, 1919, 2105, 2355
TscAI CASTG 5 cut(s) 775, 858, 948, 1200, 1721
TseFI GTSAC 1 cut(s) 853
TseI GCWGC 7 cut(s) 654, 657, 870, 1364, 2058, 2061, 2284
Tsp45I GTSAC 1 cut(s) 853
TspRI CASTG 5 cut(s) 775, 858, 948, 1200, 1721
Tth111I GACNNNGTC 1 cut(s) 20
Van91I CCANNNNNTGG 2 cut(s) 1529, 2738
VpaK11BI GGWCC 5 cut(s) 152, 1072, 1388, 1622, 2597
VspI ATTAAT 1 cut(s) 2355
XagI CCTNNNNNAGG 1 cut(s) 354
XapI RAATTY 4 cut(s) 232, 1594, 1705, 2012
XceI RCATGY 3 cut(s) 202, 691, 1672
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmnI GAANNNNTTC 2 cut(s) 484, 1307
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.