aspartic-type endopeptidase activity
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 2 cut(s) | 67, 1066 |
| AccI | GTMKAC | 2 cut(s) | 136, 760 |
| AccII | CGCG | 1 cut(s) | 291 |
| AciI | CCGC | 1 cut(s) | 548 |
| AclWI | GGATC | 2 cut(s) | 385, 935 |
| AcsI | RAATTY | 5 cut(s) | 29, 311, 438, 992, 1091 |
| AcuI | CTGAAG | 2 cut(s) | 260, 770 |
| AfaI | GTAC | 2 cut(s) | 149, 879 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 67, 554, 1066 |
| AflIII | ACRYGT | 2 cut(s) | 120, 491 |
| AgsI | TTSAA | 9 cut(s) | 34, 58, 215, 324, 602, 774, 783, 817, 885 |
| AluBI | AGCT | 4 cut(s) | 271, 287, 509, 542 |
| AluI | AGCT | 4 cut(s) | 271, 287, 509, 542 |
| Alw21I | GWGCWC | 1 cut(s) | 1083 |
| Alw26I | GTCTC | 4 cut(s) | 323, 530, 902, 930 |
| AlwI | GGATC | 2 cut(s) | 385, 935 |
| AoxI | GGCC | 1 cut(s) | 49 |
| ApeKI | GCWGC | 1 cut(s) | 271 |
| ApoI | RAATTY | 5 cut(s) | 29, 311, 438, 992, 1091 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 831, 897 |
| AspLEI | GCGC | 2 cut(s) | 293, 682 |
| AspS9I | GGNCC | 2 cut(s) | 49, 70 |
| AsuC2I | CCSGG | 1 cut(s) | 941 |
| AsuHPI | GGTGA | 1 cut(s) | 450 |
| AvaII | GGWCC | 1 cut(s) | 70 |
| BaeGI | GKGCMC | 1 cut(s) | 811 |
| Bbv12I | GWGCWC | 1 cut(s) | 1083 |
| BbvI | GCAGC | 1 cut(s) | 258 |
| BccI | CCATC | 3 cut(s) | 21, 352, 469 |
| BceAI | ACGGC | 1 cut(s) | 535 |
| BclI | TGATCA | 1 cut(s) | 115 |
| BcnI | CCSGG | 1 cut(s) | 941 |
| BcoDI | GTCTC | 4 cut(s) | 323, 530, 902, 930 |
| BfaI | CTAG | 4 cut(s) | 200, 623, 951, 968 |
| BfmI | CTRYAG | 1 cut(s) | 334 |
| BisI | GCNGC | 1 cut(s) | 272 |
| BlsI | GCNGC | 1 cut(s) | 273 |
| Bme1390I | CCNGG | 1 cut(s) | 941 |
| Bme18I | GGWCC | 1 cut(s) | 70 |
| BmgT120I | GGNCC | 2 cut(s) | 49, 70 |
| BmiI | GGNNCC | 1 cut(s) | 483 |
| BmrFI | CCNGG | 1 cut(s) | 941 |
| BmrI | ACTGGG | 1 cut(s) | 443 |
| BmsI | GCATC | 2 cut(s) | 247, 616 |
| BmuI | ACTGGG | 1 cut(s) | 443 |
| BpuMI | CCSGG | 1 cut(s) | 941 |
| BsaI | GGTCTC | 2 cut(s) | 323, 930 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 67, 554, 1066 |
| Bse1I | ACTGG | 3 cut(s) | 322, 438, 668 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 67, 554, 1066 |
| BseNI | ACTGG | 3 cut(s) | 322, 438, 668 |
| BseRI | GAGGAG | 1 cut(s) | 344 |
| BseSI | GKGCMC | 1 cut(s) | 811 |
| BseXI | GCAGC | 1 cut(s) | 258 |
| Bsh1236I | CGCG | 1 cut(s) | 291 |
| BshFI | GGCC | 1 cut(s) | 51 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1083 |
| BsiSI | CCGG | 1 cut(s) | 940 |
| BslFI | GGGAC | 1 cut(s) | 683 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 67, 554, 1066 |
| BsmAI | GTCTC | 4 cut(s) | 323, 530, 902, 930 |
| BsmFI | GGGAC | 1 cut(s) | 683 |
| BsmI | GAATGC | 1 cut(s) | 985 |
| BsnI | GGCC | 1 cut(s) | 51 |
| Bso31I | GGTCTC | 2 cut(s) | 323, 930 |
| Bsp1286I | GDGCHC | 2 cut(s) | 811, 1083 |
| Bsp1407I | TGTACA | 1 cut(s) | 147 |
| Bsp143I | GATC | 3 cut(s) | 115, 377, 927 |
| BspACI | CCGC | 1 cut(s) | 548 |
| BspANI | GGCC | 1 cut(s) | 51 |
| BspFNI | CGCG | 1 cut(s) | 291 |
| BspHI | TCATGA | 2 cut(s) | 300, 532 |
| BspLI | GGNNCC | 1 cut(s) | 483 |
| BspPI | GGATC | 2 cut(s) | 385, 935 |
| BspTNI | GGTCTC | 2 cut(s) | 323, 930 |
| BsrGI | TGTACA | 1 cut(s) | 147 |
| BsrI | ACTGG | 3 cut(s) | 322, 438, 668 |
| BssMI | GATC | 3 cut(s) | 115, 377, 927 |
| BssNAI | GTATAC | 1 cut(s) | 137 |
| Bst1107I | GTATAC | 1 cut(s) | 137 |
| Bst4CI | ACNGT | 1 cut(s) | 570 |
| BstAUI | TGTACA | 1 cut(s) | 147 |
| BstC8I | GCNNGC | 2 cut(s) | 289, 684 |
| BstFNI | CGCG | 1 cut(s) | 291 |
| BstHHI | GCGC | 2 cut(s) | 293, 682 |
| BstKTI | GATC | 3 cut(s) | 118, 380, 930 |
| BstMAI | GTCTC | 4 cut(s) | 323, 530, 902, 930 |
| BstMBI | GATC | 3 cut(s) | 115, 377, 927 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 515 |
| BstNSI | RCATGY | 2 cut(s) | 124, 495 |
| BstSCI | CCNGG | 1 cut(s) | 939 |
| BstSFI | CTRYAG | 1 cut(s) | 334 |
| BstSLI | GKGCMC | 1 cut(s) | 811 |
| BstUI | CGCG | 1 cut(s) | 291 |
| BstV1I | GCAGC | 1 cut(s) | 258 |
| BstZ17I | GTATAC | 1 cut(s) | 137 |
| BsuRI | GGCC | 1 cut(s) | 51 |
| BtsIMutI | CAGTG | 1 cut(s) | 315 |
| Cac8I | GCNNGC | 2 cut(s) | 289, 684 |
| CciI | TCATGA | 2 cut(s) | 300, 532 |
| CfoI | GCGC | 2 cut(s) | 293, 682 |
| Cfr13I | GGNCC | 2 cut(s) | 49, 70 |
| Csp6I | GTAC | 2 cut(s) | 148, 878 |
| CviAII | CATG | 7 cut(s) | 121, 230, 301, 492, 533, 755, 914 |
| CviJI | RGCY | 6 cut(s) | 51, 271, 287, 339, 509, 542 |
| CviKI_1 | RGCY | 6 cut(s) | 51, 271, 287, 339, 509, 542 |
| CviQI | GTAC | 2 cut(s) | 148, 878 |
| DpnI | GATC | 3 cut(s) | 117, 379, 929 |
| DpnII | GATC | 3 cut(s) | 115, 377, 927 |
| DraI | TTTAAA | 1 cut(s) | 997 |
| Eco31I | GGTCTC | 2 cut(s) | 323, 930 |
| Eco47I | GGWCC | 1 cut(s) | 70 |
| Eco57I | CTGAAG | 2 cut(s) | 260, 770 |
| EcoRI | GAATTC | 1 cut(s) | 29 |
| EcoT22I | ATGCAT | 1 cut(s) | 262 |
| FaeI | CATG | 7 cut(s) | 124, 233, 304, 495, 536, 758, 917 |
| FaqI | GGGAC | 1 cut(s) | 683 |
| FatI | CATG | 7 cut(s) | 120, 229, 300, 491, 532, 754, 913 |
| FauI | CCCGC | 1 cut(s) | 555 |
| FbaI | TGATCA | 1 cut(s) | 115 |
| FblI | GTMKAC | 2 cut(s) | 136, 760 |
| Fnu4HI | GCNGC | 1 cut(s) | 272 |
| Fsp4HI | GCNGC | 1 cut(s) | 272 |
| FspBI | CTAG | 4 cut(s) | 200, 623, 951, 968 |
| GlaI | GCGC | 2 cut(s) | 292, 681 |
| GluI | GCNGC | 1 cut(s) | 272 |
| HaeIII | GGCC | 1 cut(s) | 51 |
| HapII | CCGG | 1 cut(s) | 940 |
| HhaI | GCGC | 2 cut(s) | 293, 682 |
| Hin1II | CATG | 7 cut(s) | 124, 233, 304, 495, 536, 758, 917 |
| Hin6I | GCGC | 2 cut(s) | 291, 680 |
| HinP1I | GCGC | 2 cut(s) | 291, 680 |
| HinfI | GANTC | 6 cut(s) | 7, 364, 455, 779, 817, 1020 |
| HpaII | CCGG | 1 cut(s) | 940 |
| HphI | GGTGA | 1 cut(s) | 450 |
| Hpy166II | GTNNAC | 2 cut(s) | 137, 761 |
| Hpy188I | TCNGA | 1 cut(s) | 184 |
| Hpy188III | TCNNGA | 6 cut(s) | 301, 533, 574, 836, 902, 935 |
| Hpy8I | GTNNAC | 2 cut(s) | 137, 761 |
| HpyCH4III | ACNGT | 1 cut(s) | 570 |
| HpyCH4V | TGCA | 7 cut(s) | 24, 260, 518, 629, 686, 743, 983 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 515 |
| Hsp92II | CATG | 7 cut(s) | 124, 233, 304, 495, 536, 758, 917 |
| HspAI | GCGC | 2 cut(s) | 291, 680 |
| Ksp22I | TGATCA | 1 cut(s) | 115 |
| Kzo9I | GATC | 3 cut(s) | 115, 377, 927 |
| Lsp1109I | GCAGC | 1 cut(s) | 258 |
| LweI | GCATC | 2 cut(s) | 247, 616 |
| MaeI | CTAG | 4 cut(s) | 200, 623, 951, 968 |
| MaeIII | GTNAC | 4 cut(s) | 524, 649, 670, 956 |
| MalI | GATC | 3 cut(s) | 117, 379, 929 |
| MboI | GATC | 3 cut(s) | 115, 377, 927 |
| MboII | GAAGA | 3 cut(s) | 571, 589, 590 |
| MfeI | CAATTG | 2 cut(s) | 324, 812 |
| MhlI | GDGCHC | 2 cut(s) | 811, 1083 |
| MlyI | GAGTC | 2 cut(s) | 358, 1014 |
| MmeI | TCCRAC | 2 cut(s) | 207, 685 |
| MnlI | CCTC | 3 cut(s) | 139, 365, 798 |
| Mph1103I | ATGCAT | 1 cut(s) | 262 |
| MroXI | GAANNNNTTC | 2 cut(s) | 831, 897 |
| MseI | TTAA | 7 cut(s) | 173, 188, 281, 947, 974, 996, 1105 |
| MslI | CAYNNNNRTG | 1 cut(s) | 531 |
| MspI | CCGG | 1 cut(s) | 940 |
| MspR9I | CCNGG | 1 cut(s) | 941 |
| MunI | CAATTG | 2 cut(s) | 324, 812 |
| Mva1269I | GAATGC | 1 cut(s) | 985 |
| MvnI | CGCG | 1 cut(s) | 291 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 515 |
| NciI | CCSGG | 1 cut(s) | 941 |
| NdeII | GATC | 3 cut(s) | 115, 377, 927 |
| NlaIII | CATG | 7 cut(s) | 124, 233, 304, 495, 536, 758, 917 |
| NlaIV | GGNNCC | 1 cut(s) | 483 |
| NmuCI | GTSAC | 2 cut(s) | 524, 649 |
| NsiI | ATGCAT | 1 cut(s) | 262 |
| NspI | RCATGY | 2 cut(s) | 124, 495 |
| PagI | TCATGA | 2 cut(s) | 300, 532 |
| PciI | ACATGT | 2 cut(s) | 120, 491 |
| PctI | GAATGC | 1 cut(s) | 985 |
| PdmI | GAANNNNTTC | 2 cut(s) | 831, 897 |
| PfeI | GAWTC | 4 cut(s) | 7, 455, 779, 817 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 67, 1066 |
| PkrI | GCNGC | 1 cut(s) | 273 |
| PleI | GAGTC | 2 cut(s) | 358, 1014 |
| PpsI | GAGTC | 2 cut(s) | 358, 1014 |
| PscI | ACATGT | 2 cut(s) | 120, 491 |
| PspN4I | GGNNCC | 1 cut(s) | 483 |
| PspPI | GGNCC | 2 cut(s) | 49, 70 |
| RsaI | GTAC | 2 cut(s) | 149, 879 |
| RsaNI | GTAC | 2 cut(s) | 148, 878 |
| RseI | CAYNNNNRTG | 1 cut(s) | 531 |
| SaqAI | TTAA | 7 cut(s) | 173, 188, 281, 947, 974, 996, 1105 |
| SatI | GCNGC | 1 cut(s) | 272 |
| Sau3AI | GATC | 3 cut(s) | 115, 377, 927 |
| Sau96I | GGNCC | 2 cut(s) | 49, 70 |
| SchI | GAGTC | 2 cut(s) | 358, 1014 |
| ScrFI | CCNGG | 1 cut(s) | 941 |
| SduI | GDGCHC | 2 cut(s) | 811, 1083 |
| SetI | ASST | 8 cut(s) | 66, 273, 289, 335, 511, 544, 772, 990 |
| SfaNI | GCATC | 2 cut(s) | 247, 616 |
| SfcI | CTRYAG | 1 cut(s) | 334 |
| SinI | GGWCC | 1 cut(s) | 70 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 531 |
| SsiI | CCGC | 1 cut(s) | 548 |
| SspMI | CTAG | 4 cut(s) | 200, 623, 951, 968 |
| StyD4I | CCNGG | 1 cut(s) | 939 |
| TaaI | ACNGT | 1 cut(s) | 570 |
| TaqI | TCGA | 4 cut(s) | 78, 141, 790, 934 |
| TatI | WGTACW | 2 cut(s) | 147, 877 |
| TfiI | GAWTC | 4 cut(s) | 7, 455, 779, 817 |
| Tru1I | TTAA | 7 cut(s) | 173, 188, 281, 947, 974, 996, 1105 |
| Tru9I | TTAA | 7 cut(s) | 173, 188, 281, 947, 974, 996, 1105 |
| TscAI | CASTG | 1 cut(s) | 322 |
| TseFI | GTSAC | 2 cut(s) | 524, 649 |
| TseI | GCWGC | 1 cut(s) | 271 |
| Tsp45I | GTSAC | 2 cut(s) | 524, 649 |
| TspDTI | ATGAA | 9 cut(s) | 317, 521, 584, 596, 840, 857, 863, 870, 992 |
| TspRI | CASTG | 1 cut(s) | 322 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 67, 1066 |
| VpaK11BI | GGWCC | 1 cut(s) | 70 |
| XapI | RAATTY | 5 cut(s) | 29, 311, 438, 992, 1091 |
| XceI | RCATGY | 2 cut(s) | 124, 495 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 242 |
| XmiI | GTMKAC | 2 cut(s) | 136, 760 |
| XmnI | GAANNNNTTC | 2 cut(s) | 831, 897 |
| XspI | CTAG | 4 cut(s) | 200, 623, 951, 968 |
| Zsp2I | ATGCAT | 1 cut(s) | 262 |