AT4G13440

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0000286
Physical Location & Seq
Forward (+)
7810014 .. 7812610
2597 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G13440.1

Sequence Viewer

Length: 465 bp
ATGGATGAGCTCCACGAAGCCGCGATTGCTTATTACAACAATGGCTCCATGGAACAACAAAATTTGTCGTGGCAGTTTTTCCGGGCAATGGATGTTAACGGAGACGGCCGTGTCAGCCTTCAAGAATACACCGACTTCTTGCGTCAAACCGCTGGTTTAGCTTGGATCCATCCCGAGATGTTTCGGGAGCTCGACCGAAATGGAGATGGTCAGCTTGACTTTTGGGAGGTCTTGACGCTTTACTACGTGGCTCGGACCCGGACCATTAACTGCCGCACATGCCTCCGGATTCTCAATGGTCTATACTTCACGTGTGTCACATGCTTTGAATCTTCATGTGGCAACACGTTTGATCTTTGCGTCAAGTGTTATATGCGTCGGACGTATTGTCATCCGCATCGTCTCTTTTTGGATAGTTATGTCTTATTACGATCAAGACGTATCCACCATCCTTCGGTATGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6

Protein Analysis

154

Amino Acids

18.23

Weight (kDa)

6.57

Isoelectric Point (pI)

43.21

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 110
AccII CGCG 1 cut(s) 23
AccIII TCCGGA 1 cut(s) 285
AciI CCGC 4 cut(s) 21, 150, 274, 395
AclWI GGATC 2 cut(s) 160, 173
AcoI YGGCCR 1 cut(s) 106
AcsI RAATTY 1 cut(s) 61
AcvI CACGTG 1 cut(s) 312
AfiI CCNNNNNNNGG 2 cut(s) 88, 454
AflIII ACRYGT 2 cut(s) 311, 345
AgsI TTSAA 2 cut(s) 122, 329
AhdI GACNNNNNGTC 1 cut(s) 387
AluBI AGCT 4 cut(s) 10, 161, 190, 214
AluI AGCT 4 cut(s) 10, 161, 190, 214
Alw21I GWGCWC 2 cut(s) 12, 192
Alw26I GTCTC 2 cut(s) 96, 407
AlwI GGATC 2 cut(s) 160, 173
Ama87I CYCGRG 1 cut(s) 173
Aor13HI TCCGGA 1 cut(s) 285
AoxI GGCC 1 cut(s) 106
ApoI RAATTY 1 cut(s) 61
AspS9I GGNCC 2 cut(s) 255, 261
AsuC2I CCSGG 2 cut(s) 83, 259
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 2 cut(s) 255, 261
BamHI GGATCC 1 cut(s) 165
BanII GRGCYC 2 cut(s) 12, 192
BbrPI CACGTG 1 cut(s) 312
Bbv12I GWGCWC 2 cut(s) 12, 192
BccI CCATC 3 cut(s) 177, 200, 456
BceAI ACGGC 2 cut(s) 93, 121
BciVI GTATCC 1 cut(s) 452
BcnI CCSGG 2 cut(s) 83, 259
BcoDI GTCTC 2 cut(s) 96, 407
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 2 cut(s) 21, 274
BlsI GCNGC 2 cut(s) 22, 275
Bme1390I CCNGG 2 cut(s) 83, 259
Bme18I GGWCC 2 cut(s) 255, 261
BmeRI GACNNNNNGTC 1 cut(s) 387
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 2 cut(s) 255, 261
BmiI GGNNCC 3 cut(s) 46, 167, 257
BmrFI CCNGG 2 cut(s) 83, 259
BmsI GCATC 1 cut(s) 406
BpuMI CCSGG 2 cut(s) 83, 259
BsaAI YACGTR 2 cut(s) 247, 312
BsaJI CCNNGG 1 cut(s) 48
BsaWI WCCGGW 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 88, 454
Bse3DI GCAATG 1 cut(s) 93
BseAI TCCGGA 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 48
BseGI GGATG 5 cut(s) 10, 97, 169, 391, 448
BseLI CCNNNNNNNGG 2 cut(s) 88, 454
BseMI GCAATG 1 cut(s) 93
BseX3I CGGCCG 1 cut(s) 106
Bsh1236I CGCG 1 cut(s) 23
Bsh1285I CGRYCG 2 cut(s) 109, 196
BshFI GGCC 1 cut(s) 108
BsiEI CGRYCG 2 cut(s) 109, 196
BsiHKAI GWGCWC 2 cut(s) 12, 192
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 3 cut(s) 82, 259, 286
BslI CCNNNNNNNGG 2 cut(s) 88, 454
BsmAI GTCTC 2 cut(s) 96, 407
BsmBI CGTCTC 2 cut(s) 96, 407
BsnI GGCC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 173
Bsp1286I GDGCHC 2 cut(s) 12, 192
Bsp13I TCCGGA 1 cut(s) 285
Bsp143I GATC 3 cut(s) 165, 352, 431
Bsp19I CCATGG 1 cut(s) 48
BspACI CCGC 4 cut(s) 21, 150, 274, 395
BspANI GGCC 1 cut(s) 108
BspEI TCCGGA 1 cut(s) 285
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 46, 167, 257
BspPI GGATC 2 cut(s) 160, 173
BsrDI GCAATG 1 cut(s) 93
BssECI CCNNGG 1 cut(s) 48
BssMI GATC 3 cut(s) 165, 352, 431
BssT1I CCWWGG 1 cut(s) 48
BstBAI YACGTR 2 cut(s) 247, 312
BstDSI CCRYGG 1 cut(s) 48
BstF5I GGATG 5 cut(s) 10, 97, 169, 391, 448
BstFNI CGCG 1 cut(s) 23
BstKTI GATC 3 cut(s) 168, 355, 434
BstMAI GTCTC 2 cut(s) 96, 407
BstMBI GATC 3 cut(s) 165, 352, 431
BstMCI CGRYCG 2 cut(s) 109, 196
BstMWI GCNNNNNNNGC 4 cut(s) 26, 114, 158, 279
BstNSI RCATGY 2 cut(s) 282, 324
BstSCI CCNGG 2 cut(s) 81, 257
BstUI CGCG 1 cut(s) 23
BstX2I RGATCY 1 cut(s) 165
BstYI RGATCY 1 cut(s) 165
BstZI CGGCCG 1 cut(s) 106
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 1 cut(s) 108
BtgI CCRYGG 1 cut(s) 48
BtsCI GGATG 5 cut(s) 10, 97, 169, 391, 448
Cfr13I GGNCC 2 cut(s) 255, 261
CseI GACGC 4 cut(s) 131, 244, 349, 365
CviAII CATG 4 cut(s) 49, 279, 321, 336
CviJI RGCY 9 cut(s) 10, 20, 45, 108, 117, 161, 190, 214, 251
CviKI_1 RGCY 9 cut(s) 10, 20, 45, 108, 117, 161, 190, 214, 251
DpnI GATC 3 cut(s) 167, 354, 433
DpnII GATC 3 cut(s) 165, 352, 431
DrdI GACNNNNNNGTC 1 cut(s) 110
DriI GACNNNNNGTC 1 cut(s) 387
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 1 cut(s) 106
EagI CGGCCG 1 cut(s) 106
Eam1105I GACNNNNNGTC 1 cut(s) 387
Ecl136II GAGCTC 2 cut(s) 10, 190
EclXI CGGCCG 1 cut(s) 106
Eco130I CCWWGG 1 cut(s) 48
Eco24I GRGCYC 2 cut(s) 12, 192
Eco47I GGWCC 2 cut(s) 255, 261
Eco52I CGGCCG 1 cut(s) 106
Eco53kI GAGCTC 2 cut(s) 10, 190
Eco72I CACGTG 1 cut(s) 312
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 2 cut(s) 10, 190
EcoT14I CCWWGG 1 cut(s) 48
EcoT38I GRGCYC 2 cut(s) 12, 192
ErhI CCWWGG 1 cut(s) 48
Esp3I CGTCTC 2 cut(s) 96, 407
FaeI CATG 4 cut(s) 52, 282, 324, 339
FaiI YATR 9 cut(s) 50, 280, 304, 322, 337, 372, 374, 420, 460
FatI CATG 4 cut(s) 48, 278, 320, 335
Fnu4HI GCNGC 2 cut(s) 21, 274
FokI GGATG 5 cut(s) 17, 104, 156, 378, 435
FriOI GRGCYC 2 cut(s) 12, 192
Fsp4HI GCNGC 2 cut(s) 21, 274
GluI GCNGC 2 cut(s) 21, 274
HaeIII GGCC 1 cut(s) 108
HapII CCGG 3 cut(s) 82, 259, 286
HgaI GACGC 4 cut(s) 131, 244, 349, 365
Hin1II CATG 4 cut(s) 52, 282, 324, 339
HincII GTYRAC 1 cut(s) 97
HindII GTYRAC 1 cut(s) 97
HinfI GANTC 2 cut(s) 289, 329
HpaI GTTAAC 1 cut(s) 97
HpaII CCGG 3 cut(s) 82, 259, 286
Hpy166II GTNNAC 1 cut(s) 97
Hpy188I TCNGA 2 cut(s) 255, 381
Hpy188III TCNNGA 6 cut(s) 122, 173, 185, 232, 286, 435
Hpy8I GTNNAC 1 cut(s) 97
Hpy99I CGWCG 1 cut(s) 381
HpyAV CCTTC 2 cut(s) 128, 462
HpyCH4IV ACGT 5 cut(s) 246, 311, 347, 383, 439
HpyF10VI GCNNNNNNNGC 4 cut(s) 26, 114, 158, 279
HpySE526I ACGT 5 cut(s) 246, 311, 347, 383, 439
Hsp92II CATG 4 cut(s) 52, 282, 324, 339
Kpn2I TCCGGA 1 cut(s) 285
KspAI GTTAAC 1 cut(s) 97
Kzo9I GATC 3 cut(s) 165, 352, 431
LmnI GCTCC 3 cut(s) 15, 50, 187
LpnPI CCDG 4 cut(s) 95, 138, 272, 299
LweI GCATC 1 cut(s) 406
MaeII ACGT 5 cut(s) 246, 311, 347, 383, 439
MaeIII GTNAC 1 cut(s) 316
MalI GATC 3 cut(s) 167, 354, 433
MboI GATC 3 cut(s) 165, 352, 431
MboII GAAGA 1 cut(s) 324
MflI RGATCY 1 cut(s) 165
MhlI GDGCHC 2 cut(s) 12, 192
MluCI AATT 1 cut(s) 61
MmeI TCCRAC 1 cut(s) 359
MnlI CCTC 2 cut(s) 220, 293
MroI TCCGGA 1 cut(s) 285
MseI TTAA 2 cut(s) 96, 267
MspA1I CMGCKG 1 cut(s) 152
MspI CCGG 3 cut(s) 82, 259, 286
MspR9I CCNGG 2 cut(s) 83, 259
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 26, 114, 158, 279
NciI CCSGG 2 cut(s) 83, 259
NcoI CCATGG 1 cut(s) 48
NdeII GATC 3 cut(s) 165, 352, 431
NlaIII CATG 4 cut(s) 52, 282, 324, 339
NlaIV GGNNCC 3 cut(s) 46, 167, 257
NmuCI GTSAC 1 cut(s) 316
NspI RCATGY 2 cut(s) 282, 324
PcsI WCGNNNNNNNCGW 1 cut(s) 436
PfeI GAWTC 2 cut(s) 289, 329
PkrI GCNGC 2 cut(s) 22, 275
PmaCI CACGTG 1 cut(s) 312
PmlI CACGTG 1 cut(s) 312
Ppu21I YACGTR 2 cut(s) 247, 312
Psp124BI GAGCTC 2 cut(s) 12, 192
PspCI CACGTG 1 cut(s) 312
PspN4I GGNNCC 3 cut(s) 46, 167, 257
PspPI GGNCC 2 cut(s) 255, 261
PsuI RGATCY 1 cut(s) 165
SacI GAGCTC 2 cut(s) 12, 192
SaqAI TTAA 2 cut(s) 96, 267
SatI GCNGC 2 cut(s) 21, 274
Sau3AI GATC 3 cut(s) 165, 352, 431
Sau96I GGNCC 2 cut(s) 255, 261
ScrFI CCNGG 2 cut(s) 83, 259
SduI GDGCHC 2 cut(s) 12, 192
SfaNI GCATC 1 cut(s) 406
SinI GGWCC 2 cut(s) 255, 261
Sse9I AATT 1 cut(s) 61
SsiI CCGC 4 cut(s) 21, 150, 274, 395
SstI GAGCTC 2 cut(s) 12, 192
StyD4I CCNGG 2 cut(s) 81, 257
StyI CCWWGG 1 cut(s) 48
TaiI ACGT 5 cut(s) 249, 314, 350, 386, 442
TaqI TCGA 1 cut(s) 192
TaqII GACCGA 1 cut(s) 210
TasI AATT 1 cut(s) 61
TauI GCSGC 2 cut(s) 23, 276
TfiI GAWTC 2 cut(s) 289, 329
Tru1I TTAA 2 cut(s) 96, 267
Tru9I TTAA 2 cut(s) 96, 267
TseFI GTSAC 1 cut(s) 316
Tsp45I GTSAC 1 cut(s) 316
TspDTI ATGAA 1 cut(s) 324
TspGWI ACGGA 1 cut(s) 114
VpaK11BI GGWCC 2 cut(s) 255, 261
XapI RAATTY 1 cut(s) 61
XceI RCATGY 2 cut(s) 282, 324
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.