Prupe.1G049700_v2.0.a1

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp01
OG0000286
Physical Location & Seq
Forward (+)
3490342 .. 3492402
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.1G049700.1

Sequence Viewer

Length: 711 bp
ATGGAGGAGTTGCGCCAAACAGCATTTGCATATTACAAGGACGCCCCGGAGCAAATCCGGCGGTGGGTGGATGAGTTTTTCCTGGAAATGGATCATGATGAGGACGACCAAGTGAGCTTGCATGAGTTCTTGACATACATGGAGATGCATGAAGATTGTACGCATCTGAGTAACCCTAGATTCTTCGATGAGCTGAAGAAGGAAGGGAGTGAAGAGCTTGATTTCTTGGACGTGGTGACTCTCTTCTACATCGTTTATAGTGGAAAACCCTTCTGTAATGGGGATTGCAAAAAATTCGTAAAGGGAGTTTATTTTACTTGTGTAAAATGCTTTGATCATGCCAGTGATGCCGCCAACACATTCAATGTTTGTACTGCTTGTTATGTCGATGGAAAGTATGTCCATGGCCACAAGAAATTCCTTGATAACTTCCTACTGCTTCAAACCAAGAGGATGGAAGCTTTGAACCAGCTGCCAGCATCAAGCATTCATGAGTCGAAGTCACCTGAGTCTGGTAGGACATCAGAGACGAGCAATAATTCTTCTTCCCGTAGACTCACCAATGAGCCAAGCAGTTCTGGTGCATCCACTGAAGTGGTTCCCGTGAACTCAAGGCCTAAAAATAGGGCAGGGAGGAAAGCGTTGAAAACGATAGAAGTGCTTCTCGCCCTGGGGAATATCTTTGCTTCCACTCAGTGTACCATTATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

237

Amino Acids

26.91

Weight (kDa)

5.52

Isoelectric Point (pI)

55.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 553
AciI CCGC 2 cut(s) 61, 351
AclWI GGATC 1 cut(s) 99
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 2 cut(s) 293, 416
AcuI CTGAAG 2 cut(s) 215, 612
AcyI GRCGYC 1 cut(s) 42
AdeI CACNNNGTG 1 cut(s) 696
AfaI GTAC 3 cut(s) 160, 373, 700
AfiI CCNNNNNNNGG 3 cut(s) 64, 88, 512
AgsI TTSAA 4 cut(s) 364, 443, 466, 646
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 2 cut(s) 81, 669
AleI CACNNNNGTG 1 cut(s) 593
AluBI AGCT 5 cut(s) 117, 193, 217, 461, 472
AluI AGCT 5 cut(s) 117, 193, 217, 461, 472
Alw26I GTCTC 1 cut(s) 521
AlwI GGATC 1 cut(s) 99
AoxI GGCC 2 cut(s) 406, 614
ApeKI GCWGC 1 cut(s) 472
ApoI RAATTY 2 cut(s) 293, 416
Asp700I GAANNNNTTC 2 cut(s) 597, 660
AspLEI GCGC 1 cut(s) 15
AsuC2I CCSGG 1 cut(s) 47
AsuHPI GGTGA 3 cut(s) 247, 495, 550
BalI TGGCCA 1 cut(s) 408
BbvI GCAGC 1 cut(s) 459
BccI CCATC 2 cut(s) 383, 448
BcgI CGANNNNNNTGC 4 cut(s) 277, 311, 640, 674
BciT130I CCWGG 2 cut(s) 83, 671
BclI TGATCA 1 cut(s) 334
BcnI CCSGG 1 cut(s) 47
BcoDI GTCTC 1 cut(s) 521
BfaI CTAG 1 cut(s) 177
BisI GCNGC 2 cut(s) 351, 473
BlsI GCNGC 2 cut(s) 352, 474
Bme1390I CCNGG 3 cut(s) 47, 83, 671
BmgBI CACGTC 1 cut(s) 232
BmiI GGNNCC 1 cut(s) 600
BmrFI CCNGG 3 cut(s) 47, 83, 671
BmsI GCATC 5 cut(s) 135, 172, 337, 488, 593
BpuEI CTTGAG 1 cut(s) 595
BpuMI CCSGG 1 cut(s) 47
BsaHI GRCGYC 1 cut(s) 42
BsaJI CCNNGG 4 cut(s) 45, 403, 669, 670
Bsc4I CCNNNNNNNGG 3 cut(s) 64, 88, 512
Bse1I ACTGG 1 cut(s) 342
BseBI CCWGG 2 cut(s) 83, 671
BseDI CCNNGG 4 cut(s) 45, 403, 669, 670
BseGI GGATG 3 cut(s) 76, 459, 584
BseLI CCNNNNNNNGG 3 cut(s) 64, 88, 512
BseMII CTCAG 3 cut(s) 158, 498, 707
BseNI ACTGG 1 cut(s) 342
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 459
BshFI GGCC 2 cut(s) 408, 616
BsiSI CCGG 2 cut(s) 47, 58
BslI CCNNNNNNNGG 3 cut(s) 64, 88, 512
BsmAI GTCTC 1 cut(s) 521
BsmBI CGTCTC 1 cut(s) 521
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 2 cut(s) 408, 616
Bsp143I GATC 2 cut(s) 91, 334
Bsp19I CCATGG 1 cut(s) 403
BspACI CCGC 2 cut(s) 61, 351
BspANI GGCC 2 cut(s) 408, 616
BspCNI CTCAG 3 cut(s) 159, 499, 706
BspHI TCATGA 2 cut(s) 94, 490
BspLI GGNNCC 1 cut(s) 600
BspPI GGATC 1 cut(s) 99
BspQI GCTCTTC 1 cut(s) 207
BsrI ACTGG 1 cut(s) 342
BssECI CCNNGG 4 cut(s) 45, 403, 669, 670
BssMI GATC 2 cut(s) 91, 334
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 1 cut(s) 403
Bst2UI CCWGG 2 cut(s) 83, 671
Bst6I CTCTTC 2 cut(s) 207, 248
BstACI GRCGYC 1 cut(s) 42
BstC8I GCNNGC 2 cut(s) 119, 477
BstDEI CTNAG 3 cut(s) 167, 507, 693
BstDSI CCRYGG 1 cut(s) 403
BstF5I GGATG 3 cut(s) 76, 459, 584
BstHHI GCGC 1 cut(s) 15
BstKTI GATC 2 cut(s) 94, 337
BstMAI GTCTC 1 cut(s) 521
BstMBI GATC 2 cut(s) 91, 334
BstMWI GCNNNNNNNGC 2 cut(s) 58, 347
BstNI CCWGG 2 cut(s) 83, 671
BstSCI CCNGG 3 cut(s) 45, 81, 669
BstV1I GCAGC 1 cut(s) 459
BstXI CCANNNNNNTGG 2 cut(s) 454, 595
BsuRI GGCC 2 cut(s) 408, 616
BtgI CCRYGG 1 cut(s) 403
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 3 cut(s) 76, 459, 584
BtsIMutI CAGTG 3 cut(s) 349, 588, 701
Cac8I GCNNGC 2 cut(s) 119, 477
CciI TCATGA 2 cut(s) 94, 490
CfoI GCGC 1 cut(s) 15
CseI GACGC 1 cut(s) 50
Csp6I GTAC 3 cut(s) 159, 372, 699
CviAII CATG 7 cut(s) 95, 122, 139, 149, 338, 404, 491
CviJI RGCY 8 cut(s) 117, 193, 217, 408, 461, 472, 568, 616
CviKI_1 RGCY 8 cut(s) 117, 193, 217, 408, 461, 472, 568, 616
CviQI GTAC 3 cut(s) 159, 372, 699
DdeI CTNAG 3 cut(s) 167, 507, 693
DpnI GATC 2 cut(s) 93, 336
DpnII GATC 2 cut(s) 91, 334
DraIII CACNNNGTG 1 cut(s) 696
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 2 cut(s) 207, 248
EarI CTCTTC 2 cut(s) 207, 248
Eco130I CCWWGG 1 cut(s) 403
Eco147I AGGCCT 1 cut(s) 616
Eco57I CTGAAG 2 cut(s) 215, 612
EcoRII CCWGG 2 cut(s) 81, 669
EcoT14I CCWWGG 1 cut(s) 403
EcoT22I ATGCAT 1 cut(s) 150
ErhI CCWWGG 1 cut(s) 403
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 7 cut(s) 98, 125, 142, 152, 341, 407, 494
FatI CATG 7 cut(s) 94, 121, 138, 148, 337, 403, 490
FbaI TGATCA 1 cut(s) 334
FblI GTMKAC 1 cut(s) 553
Fnu4HI GCNGC 2 cut(s) 351, 473
FokI GGATG 3 cut(s) 83, 466, 571
Fsp4HI GCNGC 2 cut(s) 351, 473
FspBI CTAG 1 cut(s) 177
GlaI GCGC 1 cut(s) 14
GluI GCNGC 2 cut(s) 351, 473
HaeIII GGCC 2 cut(s) 408, 616
HapII CCGG 2 cut(s) 47, 58
HgaI GACGC 1 cut(s) 50
HhaI GCGC 1 cut(s) 15
Hin1I GRCGYC 1 cut(s) 42
Hin1II CATG 7 cut(s) 98, 125, 142, 152, 341, 407, 494
Hin6I GCGC 1 cut(s) 13
HinP1I GCGC 1 cut(s) 13
HindIII AAGCTT 1 cut(s) 459
HinfI GANTC 5 cut(s) 180, 238, 494, 509, 555
HpaII CCGG 2 cut(s) 47, 58
HphI GGTGA 3 cut(s) 247, 495, 550
Hpy166II GTNNAC 3 cut(s) 554, 607, 699
Hpy188I TCNGA 2 cut(s) 168, 526
Hpy188III TCNNGA 3 cut(s) 95, 130, 491
Hpy8I GTNNAC 3 cut(s) 554, 607, 699
HpyAV CCTTC 3 cut(s) 193, 197, 280
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 5 cut(s) 29, 121, 148, 288, 584
HpyF10VI GCNNNNNNNGC 2 cut(s) 58, 347
HpyF3I CTNAG 3 cut(s) 167, 507, 693
HpySE526I ACGT 1 cut(s) 231
Hsp92I GRCGYC 1 cut(s) 42
Hsp92II CATG 7 cut(s) 98, 125, 142, 152, 341, 407, 494
HspAI GCGC 1 cut(s) 13
Ksp22I TGATCA 1 cut(s) 334
Kzo9I GATC 2 cut(s) 91, 334
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 1 cut(s) 49
Lsp1109I GCAGC 1 cut(s) 459
LweI GCATC 5 cut(s) 135, 172, 337, 488, 593
MaeI CTAG 1 cut(s) 177
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 3 cut(s) 170, 235, 501
MalI GATC 2 cut(s) 93, 336
MboI GATC 2 cut(s) 91, 334
MboII GAAGA 7 cut(s) 164, 175, 208, 224, 235, 534, 537
MlsI TGGCCA 1 cut(s) 408
MluCI AATT 3 cut(s) 293, 416, 538
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 4 cut(s) 232, 503, 518, 549
MnlI CCTC 3 cut(s) 94, 444, 627
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 150
MroXI GAANNNNTTC 2 cut(s) 597, 660
MscI TGGCCA 1 cut(s) 408
MslI CAYNNNNRTG 3 cut(s) 143, 342, 593
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 472
MspI CCGG 2 cut(s) 47, 58
MspR9I CCNGG 3 cut(s) 47, 83, 671
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 2 cut(s) 83, 671
MwoI GCNNNNNNNGC 2 cut(s) 58, 347
NciI CCSGG 1 cut(s) 47
NcoI CCATGG 1 cut(s) 403
NdeII GATC 2 cut(s) 91, 334
NlaIII CATG 7 cut(s) 98, 125, 142, 152, 341, 407, 494
NlaIV GGNNCC 1 cut(s) 600
NmuCI GTSAC 2 cut(s) 235, 501
NsiI ATGCAT 1 cut(s) 150
OliI CACNNNNGTG 1 cut(s) 593
PagI TCATGA 2 cut(s) 94, 490
PasI CCCWGGG 1 cut(s) 670
PceI AGGCCT 1 cut(s) 616
PciSI GCTCTTC 1 cut(s) 207
PctI GAATGC 1 cut(s) 486
PdmI GAANNNNTTC 2 cut(s) 597, 660
PfeI GAWTC 1 cut(s) 180
PfoI TCCNGGA 1 cut(s) 81
PkrI GCNGC 2 cut(s) 352, 474
PleI GAGTC 4 cut(s) 232, 502, 517, 549
PpsI GAGTC 4 cut(s) 232, 502, 517, 549
Psp6I CCWGG 2 cut(s) 81, 669
PspGI CCWGG 2 cut(s) 81, 669
PspN4I GGNNCC 1 cut(s) 600
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 3 cut(s) 160, 373, 700
RsaNI GTAC 3 cut(s) 159, 372, 699
RseI CAYNNNNRTG 3 cut(s) 143, 342, 593
SapI GCTCTTC 1 cut(s) 207
SatI GCNGC 2 cut(s) 351, 473
Sau3AI GATC 2 cut(s) 91, 334
SchI GAGTC 4 cut(s) 232, 503, 518, 549
ScrFI CCNGG 3 cut(s) 47, 83, 671
SetI ASST 7 cut(s) 119, 195, 219, 234, 463, 474, 508
SfaNI GCATC 5 cut(s) 135, 172, 337, 488, 593
SmiMI CAYNNNNRTG 3 cut(s) 143, 342, 593
SmlI CTYRAG 1 cut(s) 610
SmoI CTYRAG 1 cut(s) 610
Sse9I AATT 3 cut(s) 293, 416, 538
SseBI AGGCCT 1 cut(s) 616
SsiI CCGC 2 cut(s) 61, 351
SspMI CTAG 1 cut(s) 177
StuI AGGCCT 1 cut(s) 616
StyD4I CCNGG 3 cut(s) 45, 81, 669
StyI CCWWGG 1 cut(s) 403
TaiI ACGT 1 cut(s) 234
TaqI TCGA 3 cut(s) 186, 387, 497
TasI AATT 3 cut(s) 293, 416, 538
TatI WGTACW 1 cut(s) 371
TauI GCSGC 1 cut(s) 353
TfiI GAWTC 1 cut(s) 180
TscAI CASTG 3 cut(s) 349, 595, 701
TseFI GTSAC 2 cut(s) 235, 501
TseI GCWGC 1 cut(s) 472
Tsp45I GTSAC 2 cut(s) 235, 501
TspDTI ATGAA 2 cut(s) 165, 479
TspRI CASTG 3 cut(s) 349, 595, 701
XapI RAATTY 2 cut(s) 293, 416
XmiI GTMKAC 1 cut(s) 553
XmnI GAANNNNTTC 2 cut(s) 597, 660
XspI CTAG 1 cut(s) 177
Zsp2I ATGCAT 1 cut(s) 150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.