pycom13g19690

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0000286
Physical Location & Seq
Reverse (-)
15643681 .. 15644581
901 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom13g19690.3

Sequence Viewer

Length: 579 bp
ATGGAGAGGCATGGAGATTACAAGCATCTGTGTAACTCCCTTTTTTTCGACGAACTAAAGAAGGAAGGACGTGAAGAGCTTGATTTCATGGACGTGATGACTGTCTTGTACATTATTTATAGTGGAAAACCTTTTTGTAACGGGCAGTGCAAAAAATTTATCAAGGGAGTTTATTTCGCTTGTGTCAAATGCTTTGACGATTGTGCCACTGATGCCACTAACACGTTCAATGTTTGCCCTGCTTGTTATGCTGATGGCAAATATGTCCATGGCCACGACAAATTCCTTGATAGCTTCCTGCTTCTTCAAAGCAAGAGGATGACAACTTTGAACCAGCAAGCAGCGTCAGTCATTAATGAGTCAAAGGCACTCGAGTATGGAAGTACATCGGAGACAAGCAATTCGTCTTCTCCAGCTCCTCCAGCTCCTCCTCGTCCACTCACACCTCATACATCCAATGCAATGGTTCCCGTTAATCCAAGGCCTAAAAATAGTGCCGGGAGGAAAGCATTGAAGACGATAGAAATGCTACTGGCCCTGGGAAATATCTTTGCTGCCACTCAGTGCACCATCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_5

Protein Analysis

193

Amino Acids

21.28

Weight (kDa)

8.04

Isoelectric Point (pI)

41.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 2 cut(s) 155, 281
AdeI CACNNNGTG 1 cut(s) 564
AfaI GTAC 2 cut(s) 110, 385
AflIII ACRYGT 1 cut(s) 222
AgsI TTSAA 4 cut(s) 229, 308, 331, 514
AjiI CACGTC 2 cut(s) 71, 94
AjnI CCWGG 1 cut(s) 537
AluBI AGCT 4 cut(s) 79, 294, 416, 425
AluI AGCT 4 cut(s) 79, 294, 416, 425
Alw21I GWGCWC 1 cut(s) 569
Alw26I GTCTC 1 cut(s) 386
Alw44I GTGCAC 1 cut(s) 565
Ama87I CYCGRG 1 cut(s) 371
AoxI GGCC 3 cut(s) 271, 482, 534
ApaLI GTGCAC 1 cut(s) 565
ApeKI GCWGC 2 cut(s) 341, 554
ApoI RAATTY 2 cut(s) 155, 281
ArsI GACNNNNNNTTYG 2 cut(s) 385, 417
AseI ATTAAT 1 cut(s) 354
AspS9I GGNCC 1 cut(s) 535
AsuC2I CCSGG 1 cut(s) 499
AvaI CYCGRG 1 cut(s) 371
BaeGI GKGCMC 1 cut(s) 569
BalI TGGCCA 1 cut(s) 273
BbsI GAAGAC 2 cut(s) 399, 521
Bbv12I GWGCWC 1 cut(s) 569
BbvI GCAGC 2 cut(s) 353, 541
BccI CCATC 2 cut(s) 248, 578
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BciT130I CCWGG 1 cut(s) 539
BcnI CCSGG 1 cut(s) 499
BcoDI GTCTC 1 cut(s) 386
BisI GCNGC 2 cut(s) 342, 555
BlsI GCNGC 2 cut(s) 343, 556
Bme1390I CCNGG 2 cut(s) 499, 539
BmeT110I CYCGRG 1 cut(s) 371
BmgBI CACGTC 2 cut(s) 71, 94
BmgT120I GGNCC 1 cut(s) 535
BmiI GGNNCC 1 cut(s) 468
BmrFI CCNGG 2 cut(s) 499, 539
BmsI GCATC 2 cut(s) 34, 202
BpiI GAAGAC 2 cut(s) 399, 521
BpmI CTGGAG 2 cut(s) 396, 405
BpuMI CCSGG 1 cut(s) 499
BsaJI CCNNGG 4 cut(s) 268, 479, 537, 538
Bse1I ACTGG 1 cut(s) 537
Bse3DI GCAATG 1 cut(s) 468
BseBI CCWGG 1 cut(s) 539
BseDI CCNNGG 4 cut(s) 268, 479, 537, 538
BseGI GGATG 2 cut(s) 324, 452
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 575
BseNI ACTGG 1 cut(s) 537
BseRI GAGGAG 3 cut(s) 408, 417, 420
BseSI GKGCMC 1 cut(s) 569
BseXI GCAGC 2 cut(s) 353, 541
BshFI GGCC 3 cut(s) 273, 484, 536
BsiHKAI GWGCWC 1 cut(s) 569
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 498
BsmAI GTCTC 1 cut(s) 386
BsnI GGCC 3 cut(s) 273, 484, 536
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 569
Bsp1407I TGTACA 1 cut(s) 108
Bsp19I CCATGG 1 cut(s) 268
BspANI GGCC 3 cut(s) 273, 484, 536
BspCNI CTCAG 1 cut(s) 574
BspLI GGNNCC 1 cut(s) 468
BspQI GCTCTTC 1 cut(s) 69
BsrDI GCAATG 1 cut(s) 468
BsrGI TGTACA 1 cut(s) 108
BsrI ACTGG 1 cut(s) 537
BssECI CCNNGG 4 cut(s) 268, 479, 537, 538
BssT1I CCWWGG 2 cut(s) 268, 479
Bst2UI CCWGG 1 cut(s) 539
Bst4CI ACNGT 1 cut(s) 103
Bst6I CTCTTC 1 cut(s) 69
BstAUI TGTACA 1 cut(s) 108
BstC8I GCNNGC 1 cut(s) 339
BstDEI CTNAG 1 cut(s) 561
BstDSI CCRYGG 1 cut(s) 268
BstF5I GGATG 2 cut(s) 324, 452
BstMAI GTCTC 1 cut(s) 386
BstMWI GCNNNNNNNGC 3 cut(s) 212, 248, 422
BstNI CCWGG 1 cut(s) 539
BstSCI CCNGG 2 cut(s) 497, 537
BstSLI GKGCMC 1 cut(s) 569
BstV1I GCAGC 2 cut(s) 353, 541
BstV2I GAAGAC 2 cut(s) 399, 521
BstXI CCANNNNNNTGG 1 cut(s) 463
BsuRI GGCC 3 cut(s) 273, 484, 536
BtgI CCRYGG 1 cut(s) 268
BtrI CACGTC 2 cut(s) 71, 94
BtsCI GGATG 2 cut(s) 324, 452
BtsI GCAGTG 1 cut(s) 152
BtsIMutI CAGTG 3 cut(s) 152, 207, 569
Cac8I GCNNGC 1 cut(s) 339
Cfr13I GGNCC 1 cut(s) 535
CseI GACGC 1 cut(s) 333
Csp6I GTAC 2 cut(s) 109, 384
CviAII CATG 4 cut(s) 11, 88, 269, 574
CviJI RGCY 7 cut(s) 79, 273, 294, 416, 425, 484, 536
CviKI_1 RGCY 7 cut(s) 79, 273, 294, 416, 425, 484, 536
CviQI GTAC 2 cut(s) 109, 384
DdeI CTNAG 1 cut(s) 561
DraIII CACNNNGTG 1 cut(s) 564
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 1 cut(s) 69
EarI CTCTTC 1 cut(s) 69
Eco130I CCWWGG 2 cut(s) 268, 479
Eco147I AGGCCT 1 cut(s) 484
Eco88I CYCGRG 1 cut(s) 371
EcoRII CCWGG 1 cut(s) 537
EcoT14I CCWWGG 2 cut(s) 268, 479
ErhI CCWWGG 2 cut(s) 268, 479
FaeI CATG 4 cut(s) 14, 91, 272, 577
FaiI YATR 9 cut(s) 12, 89, 120, 249, 264, 270, 378, 450, 575
FatI CATG 4 cut(s) 10, 87, 268, 573
Fnu4HI GCNGC 2 cut(s) 342, 555
FokI GGATG 2 cut(s) 331, 439
Fsp4HI GCNGC 2 cut(s) 342, 555
GluI GCNGC 2 cut(s) 342, 555
GsuI CTGGAG 2 cut(s) 396, 405
HaeIII GGCC 3 cut(s) 273, 484, 536
HapII CCGG 1 cut(s) 498
HgaI GACGC 1 cut(s) 333
Hin1II CATG 4 cut(s) 14, 91, 272, 577
HinfI GANTC 1 cut(s) 359
HpaII CCGG 1 cut(s) 498
Hpy166II GTNNAC 2 cut(s) 437, 567
Hpy188I TCNGA 1 cut(s) 391
Hpy8I GTNNAC 2 cut(s) 437, 567
Hpy99I CGWCG 1 cut(s) 53
HpyAV CCTTC 2 cut(s) 55, 59
HpyCH4III ACNGT 1 cut(s) 103
HpyCH4IV ACGT 3 cut(s) 70, 93, 224
HpyCH4V TGCA 3 cut(s) 150, 461, 567
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 248, 422
HpyF3I CTNAG 1 cut(s) 561
HpySE526I ACGT 3 cut(s) 70, 93, 224
Hsp92II CATG 4 cut(s) 14, 91, 272, 577
LguI GCTCTTC 1 cut(s) 69
LmnI GCTCC 2 cut(s) 421, 430
LpnPI CCDG 9 cut(s) 252, 311, 347, 426, 435, 511, 518, 524, 551
Lsp1109I GCAGC 2 cut(s) 353, 541
LweI GCATC 2 cut(s) 34, 202
MaeII ACGT 3 cut(s) 70, 93, 224
MaeIII GTNAC 2 cut(s) 32, 137
MboII GAAGA 4 cut(s) 86, 296, 399, 526
MhlI GDGCHC 1 cut(s) 569
MlsI TGGCCA 1 cut(s) 273
MluCI AATT 3 cut(s) 155, 281, 400
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 1 cut(s) 368
MnlI CCTC 6 cut(s) 309, 429, 438, 441, 456, 495
Mox20I TGGCCA 1 cut(s) 273
MscI TGGCCA 1 cut(s) 273
MseI TTAA 2 cut(s) 354, 474
MslI CAYNNNNRTG 2 cut(s) 92, 572
Msp20I TGGCCA 1 cut(s) 273
MspI CCGG 1 cut(s) 498
MspR9I CCNGG 2 cut(s) 499, 539
MvaI CCWGG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 212, 248, 422
NciI CCSGG 1 cut(s) 499
NcoI CCATGG 1 cut(s) 268
NlaIII CATG 4 cut(s) 14, 91, 272, 577
NlaIV GGNNCC 1 cut(s) 468
PaeR7I CTCGAG 1 cut(s) 371
PasI CCCWGGG 1 cut(s) 538
PceI AGGCCT 1 cut(s) 484
PciSI GCTCTTC 1 cut(s) 69
PkrI GCNGC 2 cut(s) 343, 556
PleI GAGTC 1 cut(s) 367
PpsI GAGTC 1 cut(s) 367
PshBI ATTAAT 1 cut(s) 354
Psp6I CCWGG 1 cut(s) 537
PspGI CCWGG 1 cut(s) 537
PspN4I GGNNCC 1 cut(s) 468
PspPI GGNCC 1 cut(s) 535
PspXI VCTCGAGB 1 cut(s) 371
RsaI GTAC 2 cut(s) 110, 385
RsaNI GTAC 2 cut(s) 109, 384
RseI CAYNNNNRTG 2 cut(s) 92, 572
SapI GCTCTTC 1 cut(s) 69
SaqAI TTAA 2 cut(s) 354, 474
SatI GCNGC 2 cut(s) 342, 555
Sau96I GGNCC 1 cut(s) 535
SchI GAGTC 1 cut(s) 368
ScrFI CCNGG 2 cut(s) 499, 539
SduI GDGCHC 1 cut(s) 569
SetI ASST 9 cut(s) 73, 81, 96, 133, 227, 296, 418, 427, 448
SfaNI GCATC 2 cut(s) 34, 202
Sfr274I CTCGAG 1 cut(s) 371
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 92, 572
SmlI CTYRAG 1 cut(s) 371
SmoI CTYRAG 1 cut(s) 371
Sse9I AATT 3 cut(s) 155, 281, 400
SseBI AGGCCT 1 cut(s) 484
StuI AGGCCT 1 cut(s) 484
StyD4I CCNGG 2 cut(s) 497, 537
StyI CCWWGG 2 cut(s) 268, 479
TaaI ACNGT 1 cut(s) 103
TaiI ACGT 3 cut(s) 73, 96, 227
TaqI TCGA 2 cut(s) 48, 372
TasI AATT 3 cut(s) 155, 281, 400
TatI WGTACW 2 cut(s) 108, 383
Tru1I TTAA 2 cut(s) 354, 474
Tru9I TTAA 2 cut(s) 354, 474
TscAI CASTG 3 cut(s) 152, 214, 569
TseI GCWGC 2 cut(s) 341, 554
TspDTI ATGAA 1 cut(s) 76
TspRI CASTG 3 cut(s) 152, 214, 569
VneI GTGCAC 1 cut(s) 565
VspI ATTAAT 1 cut(s) 354
XapI RAATTY 2 cut(s) 155, 281
XhoI CTCGAG 1 cut(s) 371
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.