Rmu_sc0005431.1_g000010

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005431.1
OG0000286
Physical Location & Seq
Reverse (-)
30057 .. 33621
3565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005431.1_g000010.1.cds

Sequence Viewer

Length: 1065 bp
atggaagaaatacgtgaagtagctttagcatactattacaattgcacaccagaacttcagggtaagacacgggaattctttcaatccatggatacgaacggcgatcagcggatctctcccagggagttcaatgaattcctccgtcagaccggctacaacacgatcattactgacccagaattctttgcaaagcttgaccgcaacggtgaccgcgggctggattttgatgaagtactcactttctactatatcattaaaactgggtatatctggtgccgaggctgcagcatgtacctttccgggctctattttacgtgcttcgactgctttgaggaagtcaacaatccctccacttttgatttatgtgatgcttgctatcgcagtcggaagtttactcaccaccacacacacttcttggagaaccacgtattgctatactacaaaagaggaccagttaacaaaatggcaatggaatatctatatgcagaagctttagagtactataataaagcaactccagagcttcagagttcagtggtgtctttctttcaatccatggttacacacggtgcaaaccaaatcagccgctctgtgttcaacgatttcctcaagcaaaaaggctacaactggataattaatgatggatacttgttccagaaactcgaccgcaaccgtgatggaagcctcgatttctgggaactgctcaccttctactacatcatcagcacaaggtacttccactgcagagaatgtcaagtgtaccttaacgggctgtattttacctgcgttgcttgctttgagggaggagctcattattgtcatccccattatgatcccggcacctataatctatgtgctgcctgctatcgcagtaggaatttcatctctcaacaccctcaccacacatactttttggataaccacatattgctgcgctccaaaagaggtcatcctccttatgctgcaccatatctggacgacttcagagagccaggagatgatcagttggcttttgggtaccttgacagacttgaagcaagtagccgtgcggaagagcttagttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

354

Amino Acids

41.89

Weight (kDa)

5.74

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 791
Acc65I GGTACC 1 cut(s) 1017
AccB1I GGYRCC 3 cut(s) 273, 839, 1017
AccBSI CCGCTC 1 cut(s) 588
AccII CGCG 1 cut(s) 213
AciI CCGC 7 cut(s) 109, 199, 211, 213, 586, 667, 1049
AclWI GGATC 2 cut(s) 119, 827
AcsI RAATTY 4 cut(s) 74, 134, 179, 877
AcuI CTGAAG 3 cut(s) 41, 509, 967
AdeI CACNNNGTG 1 cut(s) 569
AfaI GTAC 6 cut(s) 234, 293, 500, 734, 761, 1019
AfiI CCNNNNNNNGG 1 cut(s) 217
AgsI TTSAA 5 cut(s) 83, 130, 551, 598, 1034
AjnI CCWGG 2 cut(s) 119, 991
AluBI AGCT 6 cut(s) 23, 193, 491, 523, 809, 1057
AluI AGCT 6 cut(s) 23, 193, 491, 523, 809, 1057
Alw21I GWGCWC 1 cut(s) 811
AlwI GGATC 2 cut(s) 119, 827
ApeKI GCWGC 5 cut(s) 282, 285, 857, 931, 962
ApoI RAATTY 4 cut(s) 74, 134, 179, 877
AseI ATTAAT 1 cut(s) 636
Asp700I GAANNNNTTC 1 cut(s) 78
Asp718I GGTACC 1 cut(s) 1017
AspLEI GCGC 1 cut(s) 936
AspS9I GGNCC 1 cut(s) 449
AsuC2I CCSGG 2 cut(s) 301, 837
AsuHPI GGTGA 4 cut(s) 218, 389, 697, 890
AvaII GGWCC 1 cut(s) 449
BanI GGYRCC 3 cut(s) 273, 839, 1017
BanII GRGCYC 2 cut(s) 306, 811
Bbv12I GWGCWC 1 cut(s) 811
BbvI GCAGC 5 cut(s) 269, 297, 844, 918, 949
BccI CCATC 2 cut(s) 635, 671
BceAI ACGGC 2 cut(s) 115, 1029
BciT130I CCWGG 2 cut(s) 121, 993
BciVI GTATCC 2 cut(s) 85, 638
BclI TGATCA 1 cut(s) 1000
BcnI CCSGG 2 cut(s) 301, 837
BfmI CTRYAG 2 cut(s) 283, 742
BfuAI ACCTGC 1 cut(s) 791
BfuI GTATCC 2 cut(s) 85, 638
BisI GCNGC 6 cut(s) 283, 286, 586, 858, 932, 963
BlsI GCNGC 6 cut(s) 284, 287, 587, 859, 933, 964
BmcAI AGTACT 2 cut(s) 234, 500
Bme1390I CCNGG 4 cut(s) 121, 301, 837, 993
Bme18I GGWCC 1 cut(s) 449
BmgT120I GGNCC 1 cut(s) 449
BmiI GGNNCC 3 cut(s) 275, 841, 1019
BmrFI CCNGG 4 cut(s) 121, 301, 837, 993
BmrI ACTGGG 1 cut(s) 270
BmsI GCATC 1 cut(s) 358
BmuI ACTGGG 1 cut(s) 270
BpmI CTGGAG 1 cut(s) 501
BpuEI CTTGAG 1 cut(s) 593
BpuMI CCSGG 2 cut(s) 301, 837
BsaAI YACGTR 3 cut(s) 14, 315, 427
BsaJI CCNNGG 6 cut(s) 87, 119, 120, 211, 277, 555
BsaXI ACNNNNNCTCC 2 cut(s) 332, 362
Bsc4I CCNNNNNNNGG 1 cut(s) 217
Bse118I RCCGGY 1 cut(s) 149
Bse1I ACTGG 3 cut(s) 265, 452, 632
Bse3DI GCAATG 1 cut(s) 474
BseBI CCWGG 2 cut(s) 121, 993
BseDI CCNNGG 6 cut(s) 87, 119, 120, 211, 277, 555
BseGI GGATG 2 cut(s) 820, 949
BseLI CCNNNNNNNGG 1 cut(s) 217
BseMI GCAATG 1 cut(s) 474
BseNI ACTGG 3 cut(s) 265, 452, 632
BseRI GAGGAG 1 cut(s) 819
BseXI GCAGC 5 cut(s) 269, 297, 844, 918, 949
BsgI GTGCAG 1 cut(s) 948
Bsh1236I CGCG 1 cut(s) 213
Bsh1285I CGRYCG 1 cut(s) 667
BshNI GGYRCC 3 cut(s) 273, 839, 1017
BsiEI CGRYCG 1 cut(s) 667
BsiHKAI GWGCWC 1 cut(s) 811
BsiSI CCGG 3 cut(s) 150, 300, 837
BslI CCNNNNNNNGG 1 cut(s) 217
Bsp1286I GDGCHC 2 cut(s) 306, 811
Bsp143I GATC 5 cut(s) 103, 111, 162, 832, 1000
Bsp19I CCATGG 2 cut(s) 87, 555
BspACI CCGC 7 cut(s) 109, 199, 211, 213, 586, 667, 1049
BspFNI CGCG 1 cut(s) 213
BspLI GGNNCC 3 cut(s) 275, 841, 1019
BspMAI CTGCAG 2 cut(s) 287, 746
BspMI ACCTGC 1 cut(s) 791
BspPI GGATC 2 cut(s) 119, 827
BspQI GCTCTTC 1 cut(s) 1047
BspT107I GGYRCC 3 cut(s) 273, 839, 1017
BsrBI CCGCTC 1 cut(s) 588
BsrDI GCAATG 1 cut(s) 474
BsrFI RCCGGY 1 cut(s) 149
BsrI ACTGG 3 cut(s) 265, 452, 632
BssAI RCCGGY 1 cut(s) 149
BssECI CCNNGG 6 cut(s) 87, 119, 120, 211, 277, 555
BssMI GATC 5 cut(s) 103, 111, 162, 832, 1000
BssT1I CCWWGG 2 cut(s) 87, 555
Bst2UI CCWGG 2 cut(s) 121, 993
Bst4CI ACNGT 3 cut(s) 206, 569, 674
Bst6I CTCTTC 1 cut(s) 1047
BstBAI YACGTR 3 cut(s) 14, 315, 427
BstC8I GCNNGC 4 cut(s) 215, 373, 793, 862
BstDEI CTNAG 1 cut(s) 1058
BstDSI CCRYGG 3 cut(s) 87, 211, 555
BstEII GGTNACC 1 cut(s) 206
BstF5I GGATG 2 cut(s) 820, 949
BstFNI CGCG 1 cut(s) 213
BstHHI GCGC 1 cut(s) 936
BstKTI GATC 5 cut(s) 106, 114, 165, 835, 1003
BstMBI GATC 5 cut(s) 103, 111, 162, 832, 1000
BstMCI CGRYCG 1 cut(s) 667
BstMWI GCNNNNNNNGC 3 cut(s) 282, 324, 792
BstNI CCWGG 2 cut(s) 121, 993
BstNSI RCATGY 1 cut(s) 292
BstPI GGTNACC 1 cut(s) 206
BstSCI CCNGG 4 cut(s) 119, 299, 835, 991
BstSFI CTRYAG 2 cut(s) 283, 742
BstUI CGCG 1 cut(s) 213
BstV1I GCAGC 5 cut(s) 269, 297, 844, 918, 949
BstX2I RGATCY 1 cut(s) 111
BstYI RGATCY 1 cut(s) 111
BsuI GTATCC 2 cut(s) 85, 638
BtgI CCRYGG 3 cut(s) 87, 211, 555
BtsCI GGATG 2 cut(s) 820, 949
BtsI GCAGTG 1 cut(s) 739
BtsIMutI CAGTG 2 cut(s) 540, 739
BveI ACCTGC 1 cut(s) 791
Cac8I GCNNGC 4 cut(s) 215, 373, 793, 862
CfoI GCGC 1 cut(s) 936
Cfr10I RCCGGY 1 cut(s) 149
Cfr13I GGNCC 1 cut(s) 449
Cfr42I CCGCGG 1 cut(s) 214
Csp6I GTAC 6 cut(s) 233, 292, 499, 733, 760, 1018
CviAII CATG 3 cut(s) 88, 289, 556
CviQI GTAC 6 cut(s) 233, 292, 499, 733, 760, 1018
DdeI CTNAG 1 cut(s) 1058
DpnI GATC 5 cut(s) 105, 113, 164, 834, 1002
DpnII GATC 5 cut(s) 103, 111, 162, 832, 1000
DraIII CACNNNGTG 1 cut(s) 569
Eam1104I CTCTTC 1 cut(s) 1047
EarI CTCTTC 1 cut(s) 1047
Ecl136II GAGCTC 1 cut(s) 809
Eco130I CCWWGG 2 cut(s) 87, 555
Eco24I GRGCYC 2 cut(s) 306, 811
Eco47I GGWCC 1 cut(s) 449
Eco53kI GAGCTC 1 cut(s) 809
Eco57I CTGAAG 3 cut(s) 41, 509, 967
Eco91I GGTNACC 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 809
EcoO65I GGTNACC 1 cut(s) 206
EcoRI GAATTC 3 cut(s) 74, 134, 179
EcoRII CCWGG 2 cut(s) 119, 991
EcoT14I CCWWGG 2 cut(s) 87, 555
EcoT38I GRGCYC 2 cut(s) 306, 811
ErhI CCWWGG 2 cut(s) 87, 555
FaeI CATG 3 cut(s) 91, 292, 559
FalI AAGNNNNNCTT 2 cut(s) 747, 779
FatI CATG 3 cut(s) 87, 288, 555
FauI CCCGC 1 cut(s) 206
FbaI TGATCA 1 cut(s) 1000
Fnu4HI GCNGC 6 cut(s) 283, 286, 586, 858, 932, 963
FokI GGATG 2 cut(s) 807, 936
FriOI GRGCYC 2 cut(s) 306, 811
Fsp4HI GCNGC 6 cut(s) 283, 286, 586, 858, 932, 963
GlaI GCGC 1 cut(s) 935
GluI GCNGC 6 cut(s) 283, 286, 586, 858, 932, 963
GsuI CTGGAG 1 cut(s) 501
HapII CCGG 3 cut(s) 150, 300, 837
HhaI GCGC 1 cut(s) 936
Hin1II CATG 3 cut(s) 91, 292, 559
Hin6I GCGC 1 cut(s) 934
HinP1I GCGC 1 cut(s) 934
HincII GTYRAC 2 cut(s) 340, 457
HindII GTYRAC 2 cut(s) 340, 457
HindIII AAGCTT 2 cut(s) 191, 489
HpaI GTTAAC 1 cut(s) 457
HpaII CCGG 3 cut(s) 150, 300, 837
HphI GGTGA 4 cut(s) 218, 389, 697, 890
Hpy166II GTNNAC 4 cut(s) 340, 393, 457, 760
Hpy188I TCNGA 4 cut(s) 147, 387, 528, 986
Hpy188III TCNNGA 3 cut(s) 518, 655, 974
Hpy8I GTNNAC 4 cut(s) 340, 393, 457, 760
HpyAV CCTTC 1 cut(s) 718
HpyCH4III ACNGT 3 cut(s) 206, 569, 674
HpyCH4IV ACGT 3 cut(s) 13, 314, 426
HpyCH4V TGCA 7 cut(s) 45, 188, 285, 485, 572, 744, 965
HpyF10VI GCNNNNNNNGC 3 cut(s) 282, 324, 792
HpyF3I CTNAG 1 cut(s) 1058
HpySE526I ACGT 3 cut(s) 13, 314, 426
Hsp92II CATG 3 cut(s) 91, 292, 559
HspAI GCGC 1 cut(s) 934
KpnI GGTACC 1 cut(s) 1021
Ksp22I TGATCA 1 cut(s) 1000
KspAI GTTAAC 1 cut(s) 457
KspI CCGCGG 1 cut(s) 214
Kzo9I GATC 5 cut(s) 103, 111, 162, 832, 1000
LguI GCTCTTC 1 cut(s) 1047
LmnI GCTCC 2 cut(s) 806, 941
Lsp1109I GCAGC 5 cut(s) 269, 297, 844, 918, 949
LweI GCATC 1 cut(s) 358
MaeII ACGT 3 cut(s) 13, 314, 426
MaeIII GTNAC 2 cut(s) 206, 559
MalI GATC 5 cut(s) 105, 113, 164, 834, 1002
MbiI CCGCTC 1 cut(s) 588
MboI GATC 5 cut(s) 103, 111, 162, 832, 1000
MboII GAAGA 2 cut(s) 17, 1064
MfeI CAATTG 1 cut(s) 40
MflI RGATCY 1 cut(s) 111
MhlI GDGCHC 2 cut(s) 306, 811
MluCI AATT 6 cut(s) 40, 74, 134, 179, 633, 877
MmeI TCCRAC 1 cut(s) 365
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 5 cut(s) 255, 456, 636, 765, 1063
MspA1I CMGCKG 2 cut(s) 109, 213
MspI CCGG 3 cut(s) 150, 300, 837
MspR9I CCNGG 4 cut(s) 121, 301, 837, 993
MunI CAATTG 1 cut(s) 40
MvaI CCWGG 2 cut(s) 121, 993
MvnI CGCG 1 cut(s) 213
MwoI GCNNNNNNNGC 3 cut(s) 282, 324, 792
NciI CCSGG 2 cut(s) 301, 837
NcoI CCATGG 2 cut(s) 87, 555
NdeII GATC 5 cut(s) 103, 111, 162, 832, 1000
NlaIII CATG 3 cut(s) 91, 292, 559
NlaIV GGNNCC 3 cut(s) 275, 841, 1019
NmeAIII GCCGAG 1 cut(s) 302
NmuCI GTSAC 1 cut(s) 206
NspI RCATGY 1 cut(s) 292
PasI CCCWGGG 1 cut(s) 120
PciSI GCTCTTC 1 cut(s) 1047
PdmI GAANNNNTTC 1 cut(s) 78
PkrI GCNGC 6 cut(s) 284, 287, 587, 859, 933, 964
Ppu21I YACGTR 3 cut(s) 14, 315, 427
PshBI ATTAAT 1 cut(s) 636
Psp124BI GAGCTC 1 cut(s) 811
Psp6I CCWGG 2 cut(s) 119, 991
PspEI GGTNACC 1 cut(s) 206
PspGI CCWGG 2 cut(s) 119, 991
PspN4I GGNNCC 3 cut(s) 275, 841, 1019
PspPI GGNCC 1 cut(s) 449
PstI CTGCAG 2 cut(s) 287, 746
PsuI RGATCY 1 cut(s) 111
RsaI GTAC 6 cut(s) 234, 293, 500, 734, 761, 1019
RsaNI GTAC 6 cut(s) 233, 292, 499, 733, 760, 1018
SacI GAGCTC 1 cut(s) 811
SacII CCGCGG 1 cut(s) 214
SapI GCTCTTC 1 cut(s) 1047
SaqAI TTAA 5 cut(s) 255, 456, 636, 765, 1063
SatI GCNGC 6 cut(s) 283, 286, 586, 858, 932, 963
Sau3AI GATC 5 cut(s) 103, 111, 162, 832, 1000
Sau96I GGNCC 1 cut(s) 449
ScaI AGTACT 2 cut(s) 234, 500
ScrFI CCNGG 4 cut(s) 121, 301, 837, 993
SduI GDGCHC 2 cut(s) 306, 811
SfaNI GCATC 1 cut(s) 358
SfcI CTRYAG 2 cut(s) 283, 742
Sfr303I CCGCGG 1 cut(s) 214
SgrBI CCGCGG 1 cut(s) 214
SinI GGWCC 1 cut(s) 449
SmlI CTYRAG 1 cut(s) 608
SmoI CTYRAG 1 cut(s) 608
Sse9I AATT 6 cut(s) 40, 74, 134, 179, 633, 877
SsiI CCGC 7 cut(s) 109, 199, 211, 213, 586, 667, 1049
SstI GAGCTC 1 cut(s) 811
StyD4I CCNGG 4 cut(s) 119, 299, 835, 991
StyI CCWWGG 2 cut(s) 87, 555
TaaI ACNGT 3 cut(s) 206, 569, 674
TaiI ACGT 3 cut(s) 16, 317, 429
TaqI TCGA 3 cut(s) 321, 663, 687
TasI AATT 6 cut(s) 40, 74, 134, 179, 633, 877
TatI WGTACW 2 cut(s) 232, 498
TauI GCSGC 1 cut(s) 588
Tru1I TTAA 5 cut(s) 255, 456, 636, 765, 1063
Tru9I TTAA 5 cut(s) 255, 456, 636, 765, 1063
TscAI CASTG 2 cut(s) 540, 746
TseFI GTSAC 1 cut(s) 206
TseI GCWGC 5 cut(s) 282, 285, 857, 931, 962
Tsp45I GTSAC 1 cut(s) 206
TspDTI ATGAA 3 cut(s) 147, 243, 871
TspGWI ACGGA 1 cut(s) 131
TspRI CASTG 2 cut(s) 540, 746
VpaK11BI GGWCC 1 cut(s) 449
VspI ATTAAT 1 cut(s) 636
XapI RAATTY 4 cut(s) 74, 134, 179, 877
XceI RCATGY 1 cut(s) 292
XmnI GAANNNNTTC 1 cut(s) 78
ZrmI AGTACT 2 cut(s) 234, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.