Rh4AG060900

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000286
Physical Location & Seq
Forward (+)
12832438 .. 12862201
29764 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG060900.1

Sequence Viewer

Length: 465 bp
ATGGGAAGCTATTTCGACGATGACTCCGTGATGATGAAGAGAAGCTTCAAGGAGCTGGACAAGAACGGAGACGGCAAGTTGGATTTCAACGAATACAACTCTCTTTACTATCTTGTGGAGAGCGGTAGGGCTGCTTGTTGTGAATCCACATCTGAGCCATGCTCTTCTGGGTCTGATTCTGAGTCCAATCAAGTTAGTCGATCTGGATCAAAGAGAGTGCAAGTTAAGCGATCTGGATCAAAGAGGGTGAGTTTGTTTTCTTCACAAGCAGTCAACTATACTTCTTTGCATTTCAAAATTGTTTTCAGTATACTTCTTTGCCTTTTAATTAAGGTATGTAGTATTATCACGATGAATTTGACTTCAGCTAAACACATCCTGCTAGATGAACAAACACTATATAATACAAGACAAAAACATTGGTGCGTGACATCTCATCTTAAATTTCTAATTGGAGTTAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

154

Amino Acids

17.33

Weight (kDa)

8.73

Isoelectric Point (pI)

42.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 12 - 34 1e-05 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 123
AccI GTMKAC 1 cut(s) 310
AciI CCGC 1 cut(s) 123
AclWI GGATC 2 cut(s) 214, 244
AcsI RAATTY 2 cut(s) 355, 443
AcuI CTGAAG 1 cut(s) 348
AgsI TTSAA 3 cut(s) 49, 88, 295
AluBI AGCT 4 cut(s) 9, 45, 55, 368
AluI AGCT 4 cut(s) 9, 45, 55, 368
Alw26I GTCTC 1 cut(s) 63
AlwI GGATC 2 cut(s) 214, 244
ApeKI GCWGC 1 cut(s) 131
ApoI RAATTY 2 cut(s) 355, 443
AsuHPI GGTGA 1 cut(s) 259
BbvI GCAGC 1 cut(s) 118
BceAI ACGGC 1 cut(s) 88
BcoDI GTCTC 1 cut(s) 63
BfaI CTAG 1 cut(s) 383
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
BplI GAGNNNNNCTC 2 cut(s) 146, 178
BsaBI GATNNNNATC 2 cut(s) 205, 235
BsaXI ACNNNNNCTCC 2 cut(s) 8, 38
Bse8I GATNNNNATC 2 cut(s) 205, 235
BseGI GGATG 1 cut(s) 375
BseJI GATNNNNATC 2 cut(s) 205, 235
BseMII CTCAG 2 cut(s) 144, 171
BseXI GCAGC 1 cut(s) 118
BsmAI GTCTC 1 cut(s) 63
BsmBI CGTCTC 1 cut(s) 63
Bsp143I GATC 4 cut(s) 200, 206, 230, 236
BspACI CCGC 1 cut(s) 123
BspCNI CTCAG 2 cut(s) 145, 172
BspPI GGATC 2 cut(s) 214, 244
BspQI GCTCTTC 1 cut(s) 169
BsrBI CCGCTC 1 cut(s) 123
BssMI GATC 4 cut(s) 200, 206, 230, 236
BssNAI GTATAC 1 cut(s) 311
Bst1107I GTATAC 1 cut(s) 311
Bst6I CTCTTC 2 cut(s) 32, 169
BstDEI CTNAG 2 cut(s) 153, 180
BstF5I GGATG 1 cut(s) 375
BstKTI GATC 4 cut(s) 203, 209, 233, 239
BstMAI GTCTC 1 cut(s) 63
BstMBI GATC 4 cut(s) 200, 206, 230, 236
BstMWI GCNNNNNNNGC 1 cut(s) 226
BstV1I GCAGC 1 cut(s) 118
BstZ17I GTATAC 1 cut(s) 311
BtsCI GGATG 1 cut(s) 375
CviAII CATG 1 cut(s) 159
CviJI RGCY 6 cut(s) 9, 45, 55, 131, 157, 368
CviKI_1 RGCY 6 cut(s) 9, 45, 55, 131, 157, 368
DdeI CTNAG 2 cut(s) 153, 180
DpnI GATC 4 cut(s) 202, 208, 232, 238
DpnII GATC 4 cut(s) 200, 206, 230, 236
Eam1104I CTCTTC 2 cut(s) 32, 169
EarI CTCTTC 2 cut(s) 32, 169
Eco57I CTGAAG 1 cut(s) 348
Esp3I CGTCTC 1 cut(s) 63
FaeI CATG 1 cut(s) 162
FaiI YATR 6 cut(s) 160, 279, 311, 337, 400, 402
FalI AAGNNNNNCTT 2 cut(s) 29, 61
FatI CATG 1 cut(s) 158
FblI GTMKAC 1 cut(s) 310
Fnu4HI GCNGC 1 cut(s) 132
FokI GGATG 1 cut(s) 362
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 383
GluI GCNGC 1 cut(s) 132
Hin1II CATG 1 cut(s) 162
HincII GTYRAC 1 cut(s) 274
HindII GTYRAC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 43
HinfI GANTC 4 cut(s) 23, 143, 176, 182
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 2 cut(s) 274, 311
Hpy188I TCNGA 3 cut(s) 154, 175, 181
Hpy188III TCNNGA 3 cut(s) 204, 234, 349
Hpy8I GTNNAC 2 cut(s) 274, 311
Hpy99I CGWCG 1 cut(s) 20
HpyCH4V TGCA 2 cut(s) 220, 289
HpyF10VI GCNNNNNNNGC 1 cut(s) 226
HpyF3I CTNAG 2 cut(s) 153, 180
Hsp92II CATG 1 cut(s) 162
Kzo9I GATC 4 cut(s) 200, 206, 230, 236
LguI GCTCTTC 1 cut(s) 169
LmnI GCTCC 1 cut(s) 52
LpnPI CCDG 5 cut(s) 41, 153, 189, 219, 392
Lsp1109I GCAGC 1 cut(s) 118
MaeI CTAG 1 cut(s) 383
MaeIII GTNAC 1 cut(s) 427
MalI GATC 4 cut(s) 202, 208, 232, 238
MbiI CCGCTC 1 cut(s) 123
MboI GATC 4 cut(s) 200, 206, 230, 236
MboII GAAGA 3 cut(s) 49, 156, 252
MluCI AATT 6 cut(s) 297, 327, 355, 443, 450, 460
MlyI GAGTC 2 cut(s) 17, 191
MmeI TCCRAC 1 cut(s) 60
MnlI CCTC 1 cut(s) 237
MseI TTAA 5 cut(s) 225, 326, 330, 441, 459
MwoI GCNNNNNNNGC 1 cut(s) 226
NdeII GATC 4 cut(s) 200, 206, 230, 236
NlaIII CATG 1 cut(s) 162
NmuCI GTSAC 1 cut(s) 427
PacI TTAATTAA 1 cut(s) 330
PciSI GCTCTTC 1 cut(s) 169
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PfeI GAWTC 2 cut(s) 143, 176
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 2 cut(s) 17, 190
PpsI GAGTC 2 cut(s) 17, 190
SapI GCTCTTC 1 cut(s) 169
SaqAI TTAA 5 cut(s) 225, 326, 330, 441, 459
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 4 cut(s) 200, 206, 230, 236
SchI GAGTC 2 cut(s) 17, 191
SetI ASST 5 cut(s) 11, 47, 57, 336, 370
Sse9I AATT 6 cut(s) 297, 327, 355, 443, 450, 460
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 383
TaqI TCGA 2 cut(s) 15, 199
TasI AATT 6 cut(s) 297, 327, 355, 443, 450, 460
TfiI GAWTC 2 cut(s) 143, 176
Tru1I TTAA 5 cut(s) 225, 326, 330, 441, 459
Tru9I TTAA 5 cut(s) 225, 326, 330, 441, 459
TseFI GTSAC 1 cut(s) 427
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 3 cut(s) 50, 368, 402
TspGWI ACGGA 2 cut(s) 16, 81
XapI RAATTY 2 cut(s) 355, 443
XmiI GTMKAC 1 cut(s) 310
XspI CTAG 1 cut(s) 383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.