Rorug03G0355200

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000286
Physical Location & Seq
Forward (+)
43893721 .. 43895406
1686 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0355200.1

Sequence Viewer

Length: 1146 bp
ATGGCAATGACCACCGTGGCTTCCAGTCCATTTTTGTTATCCAAATCCCGTCCTTGCTCTTGTACTAATTACAACAAGAGGGGATTCCCACTTAGAGTTGCAGCTGTTGCTTCAATCCCATTTCAACAACCCATCAACGTGGACTACCTGGAACAAGAGTTCAGTGGCCGTGGAGTGGTATTCAAAGGCATTGGTGACGATTGCGTTGCCAAGATGAGTCTTGATAATGGCAGCAAAGCCATCTTGATGCTGCCAAGTGGCCTAATCGCGTCCTACAAAGCCTCCATGTGGCATGGTGGCACTGTTGAGTTGCTCCAATCTTCAGTCTCACAAGAAGAAGAAGAAGAAGAAGAAGAAGCCAATACCATCCGAGGAGGGGTGTCTGTGGCTTTGGAATGTCTCAGTCTCGGCCTCGGTCAAGAACAAGTTTCATGGTCTCCAAGTAATTGGGCTCTTCATGACATCACGGGAAATCCTCAGGACTCTATTCAGGTCGAACTGATCAGCACTGATTCCCATGACATGGTTCAAGTCAAGTACATTGTGACTCTCCAAGACGACGTCCTAATCTCACAGCTTATGGTCTCTAATAACTCTGATTCTTCGTCACTCCAACTAAGTGGGTGTATTTTAAGCCATCTGACAGTAAGCTCACCAGACGCTACTTACGCTGTTGGTTTGGAAAGATCAGATTTCTTTGGCAGGTTACCCATTTTGACAGGTTCAGCTATAATTCCTCCAGATTGTGACCAGAAAAGTGAATCTCAATTCAGCCAACTATGGAAACAAATGACATCCAATGGTTGGGGTCCTAAGAATGGCCACCAAACAGAAACTGAACAAGAAGAAGAAATGGAGGAAGGGGAAGAAGATGACAATTATAAGAACTTAAGGGAGCAAATGTGTCGAATATACACCAGTGCGCCTCGAGACTTCACAATCATTGACAGGGGTAGACGAAACTCAGTGGTGGTTGGAAGAGATGGGTTTGAAGAGCTATACATGTTCAGCCCTGGCTCAAGTCATGAATACTACGGCGAATATGCTTATATCTGCATTGGCCAGTCGGCAGTGCTCAATCCAATAATCTTGGGGCCTAAGGAATCATGGACAGGTGGACAGCGCTTACACAATCCAAATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_7 EF-hand_8

Protein Analysis

381

Amino Acids

42.01

Weight (kDa)

4.6

Isoelectric Point (pI)

60.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 882
AatII GACGTC 1 cut(s) 564
Acc36I ACCTGC 1 cut(s) 693
AccB7I CCANNNNNTGG 2 cut(s) 523, 804
AccI GTMKAC 1 cut(s) 955
AccII CGCG 1 cut(s) 269
AcoI YGGCCR 3 cut(s) 166, 820, 1060
AcuI CTGAAG 1 cut(s) 306
AcyI GRCGYC 1 cut(s) 561
AfaI GTAC 2 cut(s) 64, 539
AfeI AGCGCT 1 cut(s) 1124
AfiI CCNNNNNNNGG 6 cut(s) 175, 288, 376, 523, 804, 818
AflII CTTAAG 1 cut(s) 889
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 5 cut(s) 114, 125, 184, 530, 992
AjnI CCWGG 2 cut(s) 147, 1012
AloI GAACNNNNNNTCC 2 cut(s) 143, 175
AluBI AGCT 5 cut(s) 104, 577, 651, 728, 997
AluI AGCT 5 cut(s) 104, 577, 651, 728, 997
Alw21I GWGCWC 1 cut(s) 1077
Alw26I GTCTC 6 cut(s) 331, 404, 410, 441, 589, 924
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 1 cut(s) 927
Aor51HI AGCGCT 1 cut(s) 1124
AoxI GGCC 6 cut(s) 166, 259, 409, 820, 1060, 1094
ApeKI GCWGC 3 cut(s) 101, 231, 250
AspLEI GCGC 2 cut(s) 925, 1125
AspS9I GGNCC 2 cut(s) 809, 1094
AsuHPI GGTGA 2 cut(s) 206, 645
AvaI CYCGRG 1 cut(s) 927
AvaII GGWCC 1 cut(s) 809
AxyI CCTNAGG 2 cut(s) 477, 1098
BalI TGGCCA 2 cut(s) 822, 1062
BanII GRGCYC 1 cut(s) 454
Bbv12I GWGCWC 1 cut(s) 1077
BbvI GCAGC 3 cut(s) 113, 237, 243
BccI CCATC 5 cut(s) 140, 248, 374, 645, 977
BceAI ACGGC 2 cut(s) 153, 1051
BcgI CGANNNNNNTGC 4 cut(s) 188, 222, 887, 921
BciT130I CCWGG 2 cut(s) 149, 1014
BclI TGATCA 1 cut(s) 501
BcoDI GTCTC 6 cut(s) 331, 404, 410, 441, 589, 924
BfoI RGCGCY 1 cut(s) 1126
BfrI CTTAAG 1 cut(s) 889
BfuAI ACCTGC 1 cut(s) 693
BisI GCNGC 3 cut(s) 102, 232, 251
BlsI GCNGC 3 cut(s) 103, 233, 252
Bme1390I CCNGG 2 cut(s) 149, 1014
Bme18I GGWCC 1 cut(s) 809
BmeT110I CYCGRG 1 cut(s) 927
BmgT120I GGNCC 2 cut(s) 809, 1094
BmiI GGNNCC 2 cut(s) 810, 1095
BmrFI CCNGG 2 cut(s) 149, 1014
BmsI GCATC 1 cut(s) 237
BpmI CTGGAG 1 cut(s) 723
BpuEI CTTGAG 1 cut(s) 1003
BsaHI GRCGYC 1 cut(s) 561
BsaI GGTCTC 2 cut(s) 441, 589
BsaJI CCNNGG 5 cut(s) 15, 169, 370, 412, 1012
BsaXI ACNNNNNCTCC 4 cut(s) 266, 296, 887, 917
Bsc4I CCNNNNNNNGG 6 cut(s) 175, 288, 376, 523, 804, 818
Bse1I ACTGG 3 cut(s) 24, 918, 1063
Bse21I CCTNAGG 2 cut(s) 477, 1098
Bse3DI GCAATG 1 cut(s) 12
BseBI CCWGG 2 cut(s) 149, 1014
BseDI CCNNGG 5 cut(s) 15, 169, 370, 412, 1012
BseGI GGATG 2 cut(s) 366, 794
BseLI CCNNNNNNNGG 6 cut(s) 175, 288, 376, 523, 804, 818
BseMI GCAATG 1 cut(s) 12
BseMII CTCAG 3 cut(s) 415, 491, 978
BseNI ACTGG 3 cut(s) 24, 918, 1063
BseRI GAGGAG 1 cut(s) 387
BseXI GCAGC 3 cut(s) 113, 237, 243
Bsh1236I CGCG 1 cut(s) 269
BshFI GGCC 6 cut(s) 168, 261, 411, 822, 1062, 1096
BsiHKAI GWGCWC 1 cut(s) 1077
BsiHKCI CYCGRG 1 cut(s) 927
BslI CCNNNNNNNGG 6 cut(s) 175, 288, 376, 523, 804, 818
BsmAI GTCTC 6 cut(s) 331, 404, 410, 441, 589, 924
BsnI GGCC 6 cut(s) 168, 261, 411, 822, 1062, 1096
Bso31I GGTCTC 2 cut(s) 441, 589
BsoBI CYCGRG 1 cut(s) 927
Bsp1286I GDGCHC 2 cut(s) 454, 1077
Bsp143I GATC 2 cut(s) 501, 686
BspANI GGCC 6 cut(s) 168, 261, 411, 822, 1062, 1096
BspCNI CTCAG 3 cut(s) 414, 490, 977
BspFNI CGCG 1 cut(s) 269
BspHI TCATGA 2 cut(s) 457, 1024
BspLI GGNNCC 2 cut(s) 810, 1095
BspMI ACCTGC 1 cut(s) 693
BspQI GCTCTTC 2 cut(s) 459, 987
BspTI CTTAAG 1 cut(s) 889
BspTNI GGTCTC 2 cut(s) 441, 589
BsrDI GCAATG 1 cut(s) 12
BsrI ACTGG 3 cut(s) 24, 918, 1063
BssECI CCNNGG 5 cut(s) 15, 169, 370, 412, 1012
BssMI GATC 2 cut(s) 501, 686
BssNI GRCGYC 1 cut(s) 561
Bst2UI CCWGG 2 cut(s) 149, 1014
Bst4CI ACNGT 3 cut(s) 16, 304, 646
Bst6I CTCTTC 3 cut(s) 459, 973, 987
BstACI GRCGYC 1 cut(s) 561
BstAFI CTTAAG 1 cut(s) 889
BstAPI GCANNNNNTGC 1 cut(s) 107
BstDEI CTNAG 7 cut(s) 92, 401, 477, 617, 813, 964, 1098
BstDSI CCRYGG 2 cut(s) 15, 169
BstEII GGTNACC 1 cut(s) 705
BstF5I GGATG 2 cut(s) 366, 794
BstFNI CGCG 1 cut(s) 269
BstH2I RGCGCY 1 cut(s) 1126
BstHHI GCGC 2 cut(s) 925, 1125
BstKTI GATC 2 cut(s) 504, 689
BstMAI GTCTC 6 cut(s) 331, 404, 410, 441, 589, 924
BstMBI GATC 2 cut(s) 501, 686
BstMWI GCNNNNNNNGC 2 cut(s) 107, 668
BstNI CCWGG 2 cut(s) 149, 1014
BstNSI RCATGY 1 cut(s) 1006
BstPI GGTNACC 1 cut(s) 705
BstSCI CCNGG 2 cut(s) 147, 1012
BstUI CGCG 1 cut(s) 269
BstV1I GCAGC 3 cut(s) 113, 237, 243
BstXI CCANNNNNNTGG 3 cut(s) 139, 447, 620
Bsu36I CCTNAGG 2 cut(s) 477, 1098
BsuRI GGCC 6 cut(s) 168, 261, 411, 822, 1062, 1096
BtgI CCRYGG 2 cut(s) 15, 169
BtsCI GGATG 2 cut(s) 366, 794
BtsI GCAGTG 1 cut(s) 1077
BtsIMutI CAGTG 6 cut(s) 169, 300, 507, 925, 972, 1077
BveI ACCTGC 1 cut(s) 693
CaiI CAGNNNCTG 1 cut(s) 836
CciI TCATGA 2 cut(s) 457, 1024
CfoI GCGC 2 cut(s) 925, 1125
Cfr13I GGNCC 2 cut(s) 809, 1094
CseI GACGC 2 cut(s) 258, 668
Csp6I GTAC 2 cut(s) 63, 538
CviAII CATG 9 cut(s) 286, 293, 432, 458, 518, 523, 1003, 1025, 1107
CviQI GTAC 2 cut(s) 63, 538
DdeI CTNAG 7 cut(s) 92, 401, 477, 617, 813, 964, 1098
DpnI GATC 2 cut(s) 503, 688
DpnII GATC 2 cut(s) 501, 686
EaeI YGGCCR 3 cut(s) 166, 820, 1060
Eam1104I CTCTTC 3 cut(s) 459, 973, 987
EarI CTCTTC 3 cut(s) 459, 973, 987
Eco24I GRGCYC 1 cut(s) 454
Eco31I GGTCTC 2 cut(s) 441, 589
Eco47I GGWCC 1 cut(s) 809
Eco47III AGCGCT 1 cut(s) 1124
Eco57I CTGAAG 1 cut(s) 306
Eco81I CCTNAGG 2 cut(s) 477, 1098
Eco88I CYCGRG 1 cut(s) 927
Eco91I GGTNACC 1 cut(s) 705
EcoO109I RGGNCCY 2 cut(s) 809, 1094
EcoO65I GGTNACC 1 cut(s) 705
EcoRII CCWGG 2 cut(s) 147, 1012
EcoT38I GRGCYC 1 cut(s) 454
FaeI CATG 9 cut(s) 289, 296, 435, 461, 521, 526, 1006, 1028, 1110
FatI CATG 9 cut(s) 285, 292, 431, 457, 517, 522, 1002, 1024, 1106
FbaI TGATCA 1 cut(s) 501
FblI GTMKAC 1 cut(s) 955
Fnu4HI GCNGC 3 cut(s) 102, 232, 251
FokI GGATG 2 cut(s) 353, 781
FriOI GRGCYC 1 cut(s) 454
Fsp4HI GCNGC 3 cut(s) 102, 232, 251
GlaI GCGC 2 cut(s) 924, 1124
GluI GCNGC 3 cut(s) 102, 232, 251
GsuI CTGGAG 1 cut(s) 723
HaeII RGCGCY 1 cut(s) 1126
HaeIII GGCC 6 cut(s) 168, 261, 411, 822, 1062, 1096
HgaI GACGC 2 cut(s) 258, 668
HhaI GCGC 2 cut(s) 925, 1125
Hin1I GRCGYC 1 cut(s) 561
Hin1II CATG 9 cut(s) 289, 296, 435, 461, 521, 526, 1006, 1028, 1110
Hin6I GCGC 2 cut(s) 923, 1123
HinP1I GCGC 2 cut(s) 923, 1123
HinfI GANTC 8 cut(s) 84, 217, 482, 512, 547, 599, 761, 1103
HphI GGTGA 2 cut(s) 206, 645
Hpy166II GTNNAC 3 cut(s) 142, 956, 1118
Hpy188I TCNGA 4 cut(s) 371, 598, 642, 691
Hpy188III TCNNGA 8 cut(s) 221, 244, 419, 458, 479, 740, 929, 1025
Hpy8I GTNNAC 3 cut(s) 142, 956, 1118
Hpy99I CGWCG 1 cut(s) 563
HpyAV CCTTC 1 cut(s) 854
HpyCH4III ACNGT 3 cut(s) 16, 304, 646
HpyCH4IV ACGT 2 cut(s) 138, 561
HpyCH4V TGCA 2 cut(s) 101, 1056
HpyF10VI GCNNNNNNNGC 2 cut(s) 107, 668
HpyF3I CTNAG 7 cut(s) 92, 401, 477, 617, 813, 964, 1098
HpySE526I ACGT 2 cut(s) 138, 561
Hsp92I GRCGYC 1 cut(s) 561
Hsp92II CATG 9 cut(s) 289, 296, 435, 461, 521, 526, 1006, 1028, 1110
HspAI GCGC 2 cut(s) 923, 1123
Ksp22I TGATCA 1 cut(s) 501
Kzo9I GATC 2 cut(s) 501, 686
LguI GCTCTTC 2 cut(s) 459, 987
LmnI GCTCC 2 cut(s) 318, 895
Lsp1109I GCAGC 3 cut(s) 113, 237, 243
LweI GCATC 1 cut(s) 237
MaeII ACGT 2 cut(s) 138, 561
MaeIII GTNAC 5 cut(s) 194, 544, 606, 705, 746
MalI GATC 2 cut(s) 503, 688
MboI GATC 2 cut(s) 501, 686
MhlI GDGCHC 2 cut(s) 454, 1077
MlsI TGGCCA 2 cut(s) 822, 1062
MluCI AATT 5 cut(s) 67, 445, 732, 767, 877
MluNI TGGCCA 2 cut(s) 822, 1062
MlyI GAGTC 3 cut(s) 226, 476, 541
MmeI TCCRAC 2 cut(s) 637, 955
MnlI CCTC 9 cut(s) 72, 292, 365, 368, 422, 486, 747, 850, 936
Mox20I TGGCCA 2 cut(s) 822, 1062
MscI TGGCCA 2 cut(s) 822, 1062
MseI TTAA 2 cut(s) 632, 890
MslI CAYNNNNRTG 2 cut(s) 137, 245
Msp20I TGGCCA 2 cut(s) 822, 1062
MspA1I CMGCKG 1 cut(s) 104
MspCI CTTAAG 1 cut(s) 889
MspR9I CCNGG 2 cut(s) 149, 1014
MvaI CCWGG 2 cut(s) 149, 1014
MvnI CGCG 1 cut(s) 269
MwoI GCNNNNNNNGC 2 cut(s) 107, 668
NdeII GATC 2 cut(s) 501, 686
NlaIII CATG 9 cut(s) 289, 296, 435, 461, 521, 526, 1006, 1028, 1110
NlaIV GGNNCC 2 cut(s) 810, 1095
NmeAIII GCCGAG 1 cut(s) 387
NmuCI GTSAC 4 cut(s) 194, 544, 606, 746
NspI RCATGY 1 cut(s) 1006
PaeR7I CTCGAG 1 cut(s) 927
PagI TCATGA 2 cut(s) 457, 1024
PciI ACATGT 1 cut(s) 1002
PciSI GCTCTTC 2 cut(s) 459, 987
PfeI GAWTC 5 cut(s) 84, 512, 599, 761, 1103
PflFI GACNNNGTC 1 cut(s) 560
PflMI CCANNNNNTGG 2 cut(s) 523, 804
PkrI GCNGC 3 cut(s) 103, 233, 252
PleI GAGTC 3 cut(s) 225, 476, 541
PpsI GAGTC 3 cut(s) 225, 476, 541
PpuMI RGGWCCY 1 cut(s) 809
PscI ACATGT 1 cut(s) 1002
PsiI TTATAA 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 809
Psp6I CCWGG 2 cut(s) 147, 1012
PspEI GGTNACC 1 cut(s) 705
PspGI CCWGG 2 cut(s) 147, 1012
PspN4I GGNNCC 2 cut(s) 810, 1095
PspPI GGNCC 2 cut(s) 809, 1094
PspPPI RGGWCCY 1 cut(s) 809
PstNI CAGNNNCTG 1 cut(s) 836
PsyI GACNNNGTC 1 cut(s) 560
PvuII CAGCTG 1 cut(s) 104
RsaI GTAC 2 cut(s) 64, 539
RsaNI GTAC 2 cut(s) 63, 538
RseI CAYNNNNRTG 2 cut(s) 137, 245
SapI GCTCTTC 2 cut(s) 459, 987
SaqAI TTAA 2 cut(s) 632, 890
SatI GCNGC 3 cut(s) 102, 232, 251
Sau3AI GATC 2 cut(s) 501, 686
Sau96I GGNCC 2 cut(s) 809, 1094
SchI GAGTC 3 cut(s) 226, 476, 541
ScrFI CCNGG 2 cut(s) 149, 1014
SduI GDGCHC 2 cut(s) 454, 1077
SfaNI GCATC 1 cut(s) 237
Sfr274I CTCGAG 1 cut(s) 927
SinI GGWCC 1 cut(s) 809
SlaI CTCGAG 1 cut(s) 927
SmiMI CAYNNNNRTG 2 cut(s) 137, 245
SmlI CTYRAG 3 cut(s) 889, 927, 1018
SmoI CTYRAG 3 cut(s) 889, 927, 1018
Sse9I AATT 5 cut(s) 67, 445, 732, 767, 877
StyD4I CCNGG 2 cut(s) 147, 1012
TaaI ACNGT 3 cut(s) 16, 304, 646
TaiI ACGT 2 cut(s) 141, 564
TaqI TCGA 3 cut(s) 495, 907, 928
TaqII GACCGA 1 cut(s) 404
TasI AATT 5 cut(s) 67, 445, 732, 767, 877
TatI WGTACW 2 cut(s) 62, 537
TfiI GAWTC 5 cut(s) 84, 512, 599, 761, 1103
Tru1I TTAA 2 cut(s) 632, 890
Tru9I TTAA 2 cut(s) 632, 890
TscAI CASTG 6 cut(s) 169, 307, 514, 925, 972, 1077
TseFI GTSAC 4 cut(s) 194, 544, 606, 746
TseI GCWGC 3 cut(s) 101, 231, 250
Tsp45I GTSAC 4 cut(s) 194, 544, 606, 746
TspDTI ATGAA 3 cut(s) 420, 446, 1041
TspRI CASTG 6 cut(s) 169, 307, 514, 925, 972, 1077
Tth111I GACNNNGTC 1 cut(s) 560
Van91I CCANNNNNTGG 2 cut(s) 523, 804
Vha464I CTTAAG 1 cut(s) 889
VpaK11BI GGWCC 1 cut(s) 809
XceI RCATGY 1 cut(s) 1006
XhoI CTCGAG 1 cut(s) 927
XmiI GTMKAC 1 cut(s) 955
ZraI GACGTC 1 cut(s) 562
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.