FvH4_4g04980

EF hand

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000286
Physical Location & Seq
Forward (+)
4294684 .. 4296460
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04980.t1

Sequence Viewer

Length: 657 bp
ATGGCAATGGAAGAACTACATGCTGCTGCTTTGGCATACTACGACAATGGCACGCAACAGCTTCGGGATCTGGCAGGGTCTTTCTTCCGATCTATGGACACCAACGATGACGGTAAAATCAGCTGCTCGGAGTTCAACGAATTCCTCCAGCAAAGTGGCTACAACTGGATAATTAACGACCCTAACTTCTTCAAAAGGCTCGATCGTAACGGCGATGGTGGATTGGATTTTGGGGAAGTTCTCACTTTCTACTACATCATTAAAACGAGGTACATCCGATGCCAAGGGTACCACTGCGGCGTACACCTCTGCGGTTTGTATTTCACTTGCGTTGCTTGCTTTGATGGGGCTCATGAGCATCGCTCTACATTTGATCTATGCGCTACCTGCTATCGCAATCGGAATTACTATCACCACCAGCACCACACTTACTTCTTGGATAATCATGTATTGCTGCGCTCCAAACGAGGCCTTTCTCCGTATGCTCAACCAGATCTCAACATGGCAATAACTCCACCTCCGCCTCCAGCAGTTTACAATAATATCAATTACTACATTGCTGCTGCACCTGAAAGGCATAGTTGGTTCCAAGTATTTCGCGTGTTTGAGATGGCTCTTGCTGCTGCAAGTGTGGCTGCTAATTGTACAATCATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EF-hand_1 EF-hand_5 EF-hand_6 EF-hand_8

Protein Analysis

219

Amino Acids

25.08

Weight (kDa)

6.23

Isoelectric Point (pI)

45.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G13440 AT4G13440
fragaria_vesca FvH4_3g15000 FvH4_3g15000 FvH4_3g15020 FvH4_4g04740 FvH4_4g04740 FvH4_4g04740 FvH4_4g04760 FvH4_4g04850 FvH4_4g04860 FvH4_4g04900 FvH4_4g04980 FvH4_4g04980 FvH4_4g04990 FvH4_4g04990
malus_domestica MD05G1227800.v1.1 MD05G1228500.v1.1
prunus_persica Prupe.1G049600_v2.0.a1 Prupe.1G049700_v2.0.a1 Prupe.1G049800_v2.0.a1 Prupe.1G050000_v2.0.a1 Prupe.1G050100_v2.0.a1 Prupe.4G134500_v2.0.a1 Prupe.6G152600_v2.0.a1
pyrus_communis pycom13g19680 pycom13g19690
rosa_chinensis RchiOBHm_Chr4g0395461 RchiOBHm_Chr4g0395551 RchiOBHm_Chr4g0395571 RchiOBHm_Chr4g0395741 RchiOBHm_Chr4g0395751 RchiOBHm_Chr4g0395781 RchiOBHm_Chr4g0395801 RchiOBHm_Chr4g0397571 RchiOBHm_Chr5g0024631 RchiOBHm_Chr5g0024661
rosa_laevigata RLG00000009594 RLG00000009595 RLG00000009596 RLG00000009607 RLG00000009608 RLG00000009610 RLG00000009612 RLG00000009621 RLG00000032811
rosa_multiflora Rmu_co8376147.1_g000001 Rmu_sc0000041.1_g000020 Rmu_sc0001103.1_g000038 Rmu_sc0001757.1_g000010 Rmu_sc0004598.1_g000029 Rmu_sc0004849.1_g000001 Rmu_sc0005389.1_g000025 Rmu_sc0005431.1_g000010 Rmu_sc0005431.1_g000020 Rmu_sc0005653.1_g000008 Rmu_sc0008351.1_g000022 Rmu_sc0011701.1_g000009
rosa_roxburghii Rroxscaffold_1G00054620 Rroxscaffold_1G00054630 Rroxscaffold_1G00054640 Rroxscaffold_5G00340400 Rroxscaffold_5G00340540 Rroxscaffold_5G00340550 Rroxscaffold_5G00340590 Rroxscaffold_5G00340630 Rroxscaffold_5G00340640 Rroxscaffold_5G00340760
rosa_rugosa Rorug03G0342500 Rorug03G0354500 Rorug03G0355100 Rorug03G0355200 Rorug03G0355300 Rorug03G0356100 Rorug03G0356400 Rorug03G0356500 Rorug05G0084400
rosa_samantha Rh4AG060000 Rh4AG060900 Rh4AG061200 Rh4BG058600 Rh4BG060300 Rh4BG060500 Rh4BG061200 Rh4BG061400 Rh4BG061700 Rh4BG062000 Rh4BG062100 Rh4BG077000 Rh4CG064400 Rh4CG064600 Rh4CG065900 Rh4CG066000 Rh4CG066300 Rh4CG067400 Rh4CG068000 Rh4CG068300 Rh4CG068400 Rh4CG084600 Rh4DG056100 Rh4DG056200 Rh4DG056600 Rh4DG056800 Rh4DG056900 Rh4DG057000 Rh4DG072000 Rh5BG173500 Rh5CG189600 Rh5CG190000
rosa_wichuraiana Rw0G006720 Rw0G022980 Rw0G023000 Rw2G038350 Rw4G004810 Rw4G004850 Rw4G004870 Rw4G004880 Rw4G004990 Rw4G005000 Rw4G005080 Rw4G005110 Rw4G005120 Rw4G005130 Rw4G006450 Rw5G015940

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 395
Acc65I GGTACC 1 cut(s) 288
AccB1I GGYRCC 1 cut(s) 288
AccII CGCG 1 cut(s) 600
AciI CCGC 3 cut(s) 297, 312, 521
AclWI GGATC 1 cut(s) 75
AcsI RAATTY 1 cut(s) 140
AfaI GTAC 4 cut(s) 272, 290, 303, 646
AfiI CCNNNNNNNGG 1 cut(s) 94
AgsI TTSAA 2 cut(s) 136, 193
AluBI AGCT 2 cut(s) 61, 123
AluI AGCT 2 cut(s) 61, 123
AlwI GGATC 1 cut(s) 75
AoxI GGCC 1 cut(s) 469
ApeKI GCWGC 9 cut(s) 23, 26, 123, 454, 560, 563, 620, 623, 635
ApoI RAATTY 1 cut(s) 140
Asp718I GGTACC 1 cut(s) 288
AspLEI GCGC 2 cut(s) 383, 459
AsuHPI GGTGA 1 cut(s) 404
BanI GGYRCC 1 cut(s) 288
BanII GRGCYC 1 cut(s) 352
BbvI GCAGC 9 cut(s) 10, 13, 110, 441, 547, 550, 607, 610, 622
BccI CCATC 3 cut(s) 209, 338, 604
BceAI ACGGC 1 cut(s) 226
BcgI CGANNNNNNTGC 2 cut(s) 44, 78
BfuAI ACCTGC 1 cut(s) 395
BglII AGATCT 1 cut(s) 493
BmiI GGNNCC 2 cut(s) 290, 587
BmsI GCATC 2 cut(s) 269, 367
BplI GAGNNNNNCTC 2 cut(s) 347, 379
BpmI CTGGAG 2 cut(s) 131, 510
BsaJI CCNNGG 1 cut(s) 283
BsaXI ACNNNNNCTCC 2 cut(s) 502, 532
Bsc4I CCNNNNNNNGG 1 cut(s) 94
Bse1I ACTGG 1 cut(s) 170
Bse3DI GCAATG 2 cut(s) 12, 555
BseDI CCNNGG 1 cut(s) 283
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 1 cut(s) 94
BseMI GCAATG 2 cut(s) 12, 555
BseNI ACTGG 1 cut(s) 170
BseXI GCAGC 9 cut(s) 10, 13, 110, 441, 547, 550, 607, 610, 622
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 1 cut(s) 600
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 1 cut(s) 471
BshNI GGYRCC 1 cut(s) 288
BsiEI CGRYCG 1 cut(s) 205
BslI CCNNNNNNNGG 1 cut(s) 94
BsnI GGCC 1 cut(s) 471
Bsp1286I GDGCHC 1 cut(s) 352
Bsp1407I TGTACA 1 cut(s) 644
Bsp143I GATC 5 cut(s) 67, 89, 202, 373, 493
BspACI CCGC 3 cut(s) 297, 312, 521
BspANI GGCC 1 cut(s) 471
BspFNI CGCG 1 cut(s) 600
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 2 cut(s) 290, 587
BspMI ACCTGC 1 cut(s) 395
BspPI GGATC 1 cut(s) 75
BspT107I GGYRCC 1 cut(s) 288
BsrDI GCAATG 2 cut(s) 12, 555
BsrGI TGTACA 1 cut(s) 644
BsrI ACTGG 1 cut(s) 170
BssECI CCNNGG 1 cut(s) 283
BssMI GATC 5 cut(s) 67, 89, 202, 373, 493
BssT1I CCWWGG 1 cut(s) 283
Bst4CI ACNGT 1 cut(s) 113
BstAUI TGTACA 1 cut(s) 644
BstC8I GCNNGC 2 cut(s) 53, 337
BstF5I GGATG 1 cut(s) 273
BstFNI CGCG 1 cut(s) 600
BstHHI GCGC 2 cut(s) 383, 459
BstKTI GATC 5 cut(s) 70, 92, 205, 376, 496
BstMBI GATC 5 cut(s) 67, 89, 202, 373, 493
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 5 cut(s) 32, 336, 387, 620, 632
BstNSI RCATGY 1 cut(s) 23
BstUI CGCG 1 cut(s) 600
BstV1I GCAGC 9 cut(s) 10, 13, 110, 441, 547, 550, 607, 610, 622
BstX2I RGATCY 2 cut(s) 67, 493
BstXI CCANNNNNNTGG 1 cut(s) 155
BstYI RGATCY 2 cut(s) 67, 493
BsuRI GGCC 1 cut(s) 471
BtgZI GCGATG 2 cut(s) 228, 344
BtsCI GGATG 1 cut(s) 273
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 292
BveI ACCTGC 1 cut(s) 395
Cac8I GCNNGC 2 cut(s) 53, 337
CciI TCATGA 1 cut(s) 352
CfoI GCGC 2 cut(s) 383, 459
Csp6I GTAC 4 cut(s) 271, 289, 302, 645
CviAII CATG 5 cut(s) 20, 353, 446, 502, 652
CviJI RGCY 8 cut(s) 61, 123, 159, 199, 350, 471, 614, 635
CviKI_1 RGCY 8 cut(s) 61, 123, 159, 199, 350, 471, 614, 635
CviQI GTAC 4 cut(s) 271, 289, 302, 645
DpnI GATC 5 cut(s) 69, 91, 204, 375, 495
DpnII GATC 5 cut(s) 67, 89, 202, 373, 493
EciI GGCGGA 1 cut(s) 510
Eco130I CCWWGG 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 352
EcoRI GAATTC 1 cut(s) 140
EcoT14I CCWWGG 1 cut(s) 283
EcoT38I GRGCYC 1 cut(s) 352
ErhI CCWWGG 1 cut(s) 283
FaeI CATG 5 cut(s) 23, 356, 449, 505, 655
FatI CATG 5 cut(s) 19, 352, 445, 501, 651
FokI GGATG 1 cut(s) 260
FriOI GRGCYC 1 cut(s) 352
GlaI GCGC 2 cut(s) 382, 458
GsuI CTGGAG 2 cut(s) 131, 510
HaeIII GGCC 1 cut(s) 471
HhaI GCGC 2 cut(s) 383, 459
Hin1II CATG 5 cut(s) 23, 356, 449, 505, 655
Hin6I GCGC 2 cut(s) 381, 457
HinP1I GCGC 2 cut(s) 381, 457
HphI GGTGA 1 cut(s) 404
Hpy166II GTNNAC 2 cut(s) 304, 535
Hpy188I TCNGA 4 cut(s) 89, 130, 278, 402
Hpy188III TCNNGA 2 cut(s) 65, 353
Hpy8I GTNNAC 2 cut(s) 304, 535
HpyCH4III ACNGT 1 cut(s) 113
HpyCH4V TGCA 2 cut(s) 566, 626
HpyF10VI GCNNNNNNNGC 5 cut(s) 32, 336, 387, 620, 632
Hsp92II CATG 5 cut(s) 23, 356, 449, 505, 655
HspAI GCGC 2 cut(s) 381, 457
KpnI GGTACC 1 cut(s) 292
Kzo9I GATC 5 cut(s) 67, 89, 202, 373, 493
LmnI GCTCC 1 cut(s) 464
LpnPI CCDG 9 cut(s) 56, 60, 151, 161, 400, 431, 504, 540, 582
Lsp1109I GCAGC 9 cut(s) 10, 13, 110, 441, 547, 550, 607, 610, 622
LweI GCATC 2 cut(s) 269, 367
MaeIII GTNAC 1 cut(s) 206
MalI GATC 5 cut(s) 69, 91, 204, 375, 495
MboI GATC 5 cut(s) 67, 89, 202, 373, 493
MboII GAAGA 3 cut(s) 23, 76, 181
MflI RGATCY 2 cut(s) 67, 493
MhlI GDGCHC 1 cut(s) 352
MluCI AATT 5 cut(s) 140, 171, 403, 547, 640
MnlI CCTC 6 cut(s) 155, 261, 317, 461, 528, 534
MseI TTAA 2 cut(s) 174, 261
MspA1I CMGCKG 1 cut(s) 123
MvnI CGCG 1 cut(s) 600
MwoI GCNNNNNNNGC 5 cut(s) 32, 336, 387, 620, 632
NdeII GATC 5 cut(s) 67, 89, 202, 373, 493
NlaIII CATG 5 cut(s) 23, 356, 449, 505, 655
NlaIV GGNNCC 2 cut(s) 290, 587
NspI RCATGY 1 cut(s) 23
PagI TCATGA 1 cut(s) 352
PceI AGGCCT 1 cut(s) 471
Ple19I CGATCG 1 cut(s) 205
PspN4I GGNNCC 2 cut(s) 290, 587
PsuI RGATCY 2 cut(s) 67, 493
PvuI CGATCG 1 cut(s) 205
PvuII CAGCTG 1 cut(s) 123
RsaI GTAC 4 cut(s) 272, 290, 303, 646
RsaNI GTAC 4 cut(s) 271, 289, 302, 645
SaqAI TTAA 2 cut(s) 174, 261
Sau3AI GATC 5 cut(s) 67, 89, 202, 373, 493
SduI GDGCHC 1 cut(s) 352
SetI ASST 7 cut(s) 63, 125, 272, 309, 389, 520, 571
SfaNI GCATC 2 cut(s) 269, 367
Sse9I AATT 5 cut(s) 140, 171, 403, 547, 640
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 3 cut(s) 297, 312, 521
StuI AGGCCT 1 cut(s) 471
StyI CCWWGG 1 cut(s) 283
TaaI ACNGT 1 cut(s) 113
TaqI TCGA 1 cut(s) 201
TasI AATT 5 cut(s) 140, 171, 403, 547, 640
TatI WGTACW 1 cut(s) 644
TauI GCSGC 1 cut(s) 300
Tru1I TTAA 2 cut(s) 174, 261
Tru9I TTAA 2 cut(s) 174, 261
TscAI CASTG 1 cut(s) 299
TseI GCWGC 9 cut(s) 23, 26, 123, 454, 560, 563, 620, 623, 635
TspGWI ACGGA 1 cut(s) 468
TspRI CASTG 1 cut(s) 299
XapI RAATTY 1 cut(s) 140
XceI RCATGY 1 cut(s) 23
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.